[{"month":"06","main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2020.05.13.094359"}],"doi":"10.1038/s41556-021-00700-2","pmid":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","abstract":[{"text":"Intestinal organoids derived from single cells undergo complex crypt–villus patterning and morphogenesis. However, the nature and coordination of the underlying forces remains poorly characterized. Here, using light-sheet microscopy and large-scale imaging quantification, we demonstrate that crypt formation coincides with a stark reduction in lumen volume. We develop a 3D biophysical model to computationally screen different mechanical scenarios of crypt morphogenesis. Combining this with live-imaging data and multiple mechanical perturbations, we show that actomyosin-driven crypt apical contraction and villus basal tension work synergistically with lumen volume reduction to drive crypt morphogenesis, and demonstrate the existence of a critical point in differential tensions above which crypt morphology becomes robust to volume changes. Finally, we identified a sodium/glucose cotransporter that is specific to differentiated enterocytes that modulates lumen volume reduction through cell swelling in the villus region. Together, our study uncovers the cellular basis of how cell fate modulates osmotic and actomyosin forces to coordinate robust morphogenesis.","lang":"eng"}],"date_published":"2021-06-21T00:00:00Z","status":"public","day":"21","corr_author":"1","date_updated":"2025-04-14T07:52:26Z","oa_version":"Preprint","language":[{"iso":"eng"}],"ec_funded":1,"oa":1,"acknowledgement":"We acknowledge the members of the Lennon-Duménil laboratory for sharing the mouse line of Myh9-GFP. We are grateful to the members of the Liberali laboratory and the FMI facilities for their support. We thank E. Tagliavini for IT support; L. Gelman for assistance and training; S. Bichet and A. Bogucki for helping with histology of mouse tissues; H. Kohler for fluorescence-activated cell sorting; G. Q. G. de Medeiros for maintenance of light-sheet microscopy; M. G. Stadler for scRNA-seq analysis; G. Gay for discussions on the 3D vertex model; the members of the Liberali laboratory, C. P. Heisenberg and C. Tsiairis for reading and providing feedback on the manuscript. Funding: Q.Y. is supported by a Postdoc fellowship from Peter und Taul Engelhorn Stiftung (PTES). This work received funding from the European Research Council (ERC) under the EU Horizon 2020 research and Innovation Programme Grant Agreement no. 758617 (to P.L.), the Swiss National Foundation (SNF) (POOP3_157531, to P.L.) and from the ERC under the EU Horizon 2020 Research and Innovation Program Grant Agreements 851288 (to E.H.) and the Austrian Science Fund (FWF) (P31639, to E.H.).","date_created":"2021-07-04T22:01:25Z","article_processing_charge":"No","publication_identifier":{"eissn":["1476-4679"],"issn":["1465-7392"]},"department":[{"_id":"EdHa"}],"page":"733–744","title":"Cell fate coordinates mechano-osmotic forces in intestinal crypt formation","external_id":{"pmid":["34155381"],"isi":["000664016300003"]},"article_type":"original","volume":23,"year":"2021","project":[{"grant_number":"851288","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis"},{"call_identifier":"FWF","grant_number":"P31639","_id":"268294B6-B435-11E9-9278-68D0E5697425","name":"Active mechano-chemical description of the cell cytoskeleton"}],"publication":"Nature Cell Biology","intvolume":"        23","publisher":"Springer Nature","type":"journal_article","isi":1,"quality_controlled":"1","scopus_import":"1","citation":{"chicago":"Yang, Qiutan, Shi-lei Xue, Chii Jou Chan, Markus Rempfler, Dario Vischi, Francisca Maurer-Gutierrez, Takashi Hiiragi, Edouard B Hannezo, and Prisca Liberali. “Cell Fate Coordinates Mechano-Osmotic Forces in Intestinal Crypt Formation.” <i>Nature Cell Biology</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41556-021-00700-2\">https://doi.org/10.1038/s41556-021-00700-2</a>.","short":"Q. Yang, S. Xue, C.J. Chan, M. Rempfler, D. Vischi, F. Maurer-Gutierrez, T. Hiiragi, E.B. Hannezo, P. Liberali, Nature Cell Biology 23 (2021) 733–744.","apa":"Yang, Q., Xue, S., Chan, C. J., Rempfler, M., Vischi, D., Maurer-Gutierrez, F., … Liberali, P. (2021). Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41556-021-00700-2\">https://doi.org/10.1038/s41556-021-00700-2</a>","ama":"Yang Q, Xue S, Chan CJ, et al. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. <i>Nature Cell Biology</i>. 2021;23:733–744. doi:<a href=\"https://doi.org/10.1038/s41556-021-00700-2\">10.1038/s41556-021-00700-2</a>","mla":"Yang, Qiutan, et al. “Cell Fate Coordinates Mechano-Osmotic Forces in Intestinal Crypt Formation.” <i>Nature Cell Biology</i>, vol. 23, Springer Nature, 2021, pp. 733–744, doi:<a href=\"https://doi.org/10.1038/s41556-021-00700-2\">10.1038/s41556-021-00700-2</a>.","ieee":"Q. Yang <i>et al.</i>, “Cell fate coordinates mechano-osmotic forces in intestinal crypt formation,” <i>Nature Cell Biology</i>, vol. 23. Springer Nature, pp. 733–744, 2021.","ista":"Yang Q, Xue S, Chan CJ, Rempfler M, Vischi D, Maurer-Gutierrez F, Hiiragi T, Hannezo EB, Liberali P. 2021. Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. Nature Cell Biology. 23, 733–744."},"author":[{"full_name":"Yang, Qiutan","last_name":"Yang","first_name":"Qiutan"},{"full_name":"Xue, Shi-lei","first_name":"Shi-lei","last_name":"Xue","id":"31D2C804-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Chii Jou","last_name":"Chan","full_name":"Chan, Chii Jou"},{"full_name":"Rempfler, Markus","first_name":"Markus","last_name":"Rempfler"},{"last_name":"Vischi","first_name":"Dario","full_name":"Vischi, Dario"},{"first_name":"Francisca","last_name":"Maurer-Gutierrez","full_name":"Maurer-Gutierrez, Francisca"},{"first_name":"Takashi","last_name":"Hiiragi","full_name":"Hiiragi, Takashi"},{"orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B"},{"first_name":"Prisca","last_name":"Liberali","full_name":"Liberali, Prisca"}],"_id":"9629"},{"department":[{"_id":"CaHe"},{"_id":"EdHa"}],"publication_identifier":{"issn":["1465-7392"]},"acknowledged_ssus":[{"_id":"Bio"}],"date_created":"2018-12-30T22:59:15Z","article_processing_charge":"No","year":"2019","publication":"Nature Cell Biology","project":[{"call_identifier":"H2020","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"},{"_id":"253E54C8-B435-11E9-9278-68D0E5697425","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants","grant_number":"ALTF710-2016"}],"external_id":{"isi":["000457468300011"],"pmid":["30559456"]},"title":"Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling","article_type":"original","volume":21,"related_material":{"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/when-a-fish-becomes-fluid/"}]},"page":"169–178","type":"journal_article","isi":1,"publisher":"Nature Publishing Group","intvolume":"        21","author":[{"full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195","last_name":"Petridou","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","first_name":"Nicoletta"},{"last_name":"Grigolon","first_name":"Silvia","full_name":"Grigolon, Silvia"},{"full_name":"Salbreux, Guillaume","first_name":"Guillaume","last_name":"Salbreux"},{"full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"_id":"5789","file_date_updated":"2020-10-21T07:18:35Z","citation":{"chicago":"Petridou, Nicoletta, Silvia Grigolon, Guillaume Salbreux, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2019. <a href=\"https://doi.org/10.1038/s41556-018-0247-4\">https://doi.org/10.1038/s41556-018-0247-4</a>.","short":"N. Petridou, S. Grigolon, G. Salbreux, E.B. Hannezo, C.-P.J. Heisenberg, Nature Cell Biology 21 (2019) 169–178.","apa":"Petridou, N., Grigolon, S., Salbreux, G., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2019). Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41556-018-0247-4\">https://doi.org/10.1038/s41556-018-0247-4</a>","ama":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. <i>Nature Cell Biology</i>. 2019;21:169–178. doi:<a href=\"https://doi.org/10.1038/s41556-018-0247-4\">10.1038/s41556-018-0247-4</a>","mla":"Petridou, Nicoletta, et al. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” <i>Nature Cell Biology</i>, vol. 21, Nature Publishing Group, 2019, pp. 169–178, doi:<a href=\"https://doi.org/10.1038/s41556-018-0247-4\">10.1038/s41556-018-0247-4</a>.","ieee":"N. Petridou, S. Grigolon, G. Salbreux, E. B. Hannezo, and C.-P. J. Heisenberg, “Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling,” <i>Nature Cell Biology</i>, vol. 21. Nature Publishing Group, pp. 169–178, 2019.","ista":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. 2019. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 21, 169–178."},"has_accepted_license":"1","scopus_import":"1","quality_controlled":"1","doi":"10.1038/s41556-018-0247-4","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"02","status":"public","date_published":"2019-02-01T00:00:00Z","abstract":[{"lang":"eng","text":"Tissue morphogenesis is driven by mechanical forces that elicit changes in cell size, shape and motion. The extent by which forces deform tissues critically depends on the rheological properties of the recipient tissue. Yet, whether and how dynamic changes in tissue rheology affect tissue morphogenesis and how they are regulated within the developing organism remain unclear. Here, we show that blastoderm spreading at the onset of zebrafish morphogenesis relies on a rapid, pronounced and spatially patterned tissue fluidization. Blastoderm fluidization is temporally controlled by mitotic cell rounding-dependent cell–cell contact disassembly during the last rounds of cell cleavages. Moreover, fluidization is spatially restricted to the central blastoderm by local activation of non-canonical Wnt signalling within the blastoderm margin, increasing cell cohesion and thereby counteracting the effect of mitotic rounding on contact disassembly. Overall, our results identify a fluidity transition mediated by loss of cell cohesion as a critical regulator of embryo morphogenesis."}],"publication_status":"published","ddc":["570"],"file":[{"date_updated":"2020-10-21T07:18:35Z","access_level":"open_access","creator":"dernst","relation":"main_file","content_type":"application/pdf","file_id":"8685","date_created":"2020-10-21T07:18:35Z","checksum":"e38523787b3bc84006f2793de99ad70f","file_size":71590590,"file_name":"2018_NatureCellBio_Petridou_accepted.pdf","success":1}],"day":"01","oa":1,"ec_funded":1,"language":[{"iso":"eng"}],"date_updated":"2025-07-10T11:52:59Z","oa_version":"Submitted Version"},{"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025891","open_access":"1"}],"month":"11","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.1038/s41556-019-0411-5","pmid":1,"publication_status":"published","abstract":[{"text":"Cell migration is hypothesized to involve a cycle of behaviours beginning with leading edge extension. However, recent evidence suggests that the leading edge may be dispensable for migration, raising the question of what actually controls cell directionality. Here, we exploit the embryonic migration of Drosophila macrophages to bridge the different temporal scales of the behaviours controlling motility. This approach reveals that edge fluctuations during random motility are not persistent and are weakly correlated with motion. In contrast, flow of the actin network behind the leading edge is highly persistent. Quantification of actin flow structure during migration reveals a stable organization and asymmetry in the cell-wide flowfield that strongly correlates with cell directionality. This organization is regulated by a gradient of actin network compression and destruction, which is controlled by myosin contraction and cofilin-mediated disassembly. It is this stable actin-flow polarity, which integrates rapid fluctuations of the leading edge, that controls inherent cellular persistence.","lang":"eng"}],"status":"public","date_published":"2019-11-01T00:00:00Z","day":"01","oa_version":"Submitted Version","date_updated":"2023-09-06T11:08:52Z","issue":"11","language":[{"iso":"eng"}],"oa":1,"publication_identifier":{"eissn":["1476-4679"],"issn":["1465-7392"]},"article_processing_charge":"No","date_created":"2019-11-25T08:55:00Z","department":[{"_id":"MiSi"}],"page":"1370-1381","publication":"Nature Cell Biology","year":"2019","volume":21,"title":"Persistent and polarized global actin flow is essential for directionality during cell migration","external_id":{"pmid":["31685997"],"isi":["000495888300009"]},"article_type":"original","publisher":"Springer Nature","intvolume":"        21","isi":1,"type":"journal_article","citation":{"mla":"Yolland, Lawrence, et al. “Persistent and Polarized Global Actin Flow Is Essential for Directionality during Cell Migration.” <i>Nature Cell Biology</i>, vol. 21, no. 11, Springer Nature, 2019, pp. 1370–81, doi:<a href=\"https://doi.org/10.1038/s41556-019-0411-5\">10.1038/s41556-019-0411-5</a>.","ista":"Yolland L, Burki M, Marcotti S, Luchici A, Kenny FN, Davis JR, Serna-Morales E, Müller J, Sixt MK, Davidson A, Wood W, Schumacher LJ, Endres RG, Miodownik M, Stramer BM. 2019. Persistent and polarized global actin flow is essential for directionality during cell migration. Nature Cell Biology. 21(11), 1370–1381.","ieee":"L. Yolland <i>et al.</i>, “Persistent and polarized global actin flow is essential for directionality during cell migration,” <i>Nature Cell Biology</i>, vol. 21, no. 11. Springer Nature, pp. 1370–1381, 2019.","ama":"Yolland L, Burki M, Marcotti S, et al. Persistent and polarized global actin flow is essential for directionality during cell migration. <i>Nature Cell Biology</i>. 2019;21(11):1370-1381. doi:<a href=\"https://doi.org/10.1038/s41556-019-0411-5\">10.1038/s41556-019-0411-5</a>","short":"L. Yolland, M. Burki, S. Marcotti, A. Luchici, F.N. Kenny, J.R. Davis, E. Serna-Morales, J. Müller, M.K. Sixt, A. Davidson, W. Wood, L.J. Schumacher, R.G. Endres, M. Miodownik, B.M. Stramer, Nature Cell Biology 21 (2019) 1370–1381.","apa":"Yolland, L., Burki, M., Marcotti, S., Luchici, A., Kenny, F. N., Davis, J. R., … Stramer, B. M. (2019). Persistent and polarized global actin flow is essential for directionality during cell migration. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41556-019-0411-5\">https://doi.org/10.1038/s41556-019-0411-5</a>","chicago":"Yolland, Lawrence, Mubarik Burki, Stefania Marcotti, Andrei Luchici, Fiona N. Kenny, John Robert Davis, Eduardo Serna-Morales, et al. “Persistent and Polarized Global Actin Flow Is Essential for Directionality during Cell Migration.” <i>Nature Cell Biology</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41556-019-0411-5\">https://doi.org/10.1038/s41556-019-0411-5</a>."},"quality_controlled":"1","scopus_import":"1","_id":"7105","author":[{"full_name":"Yolland, Lawrence","last_name":"Yolland","first_name":"Lawrence"},{"full_name":"Burki, Mubarik","first_name":"Mubarik","last_name":"Burki"},{"full_name":"Marcotti, Stefania","first_name":"Stefania","last_name":"Marcotti"},{"full_name":"Luchici, Andrei","first_name":"Andrei","last_name":"Luchici"},{"full_name":"Kenny, Fiona N.","last_name":"Kenny","first_name":"Fiona N."},{"full_name":"Davis, John Robert","first_name":"John Robert","last_name":"Davis"},{"full_name":"Serna-Morales, Eduardo","last_name":"Serna-Morales","first_name":"Eduardo"},{"first_name":"Jan","last_name":"Müller","id":"AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D","full_name":"Müller, Jan"},{"full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K"},{"full_name":"Davidson, Andrew","last_name":"Davidson","first_name":"Andrew"},{"last_name":"Wood","first_name":"Will","full_name":"Wood, Will"},{"first_name":"Linus J.","last_name":"Schumacher","full_name":"Schumacher, Linus J."},{"last_name":"Endres","first_name":"Robert G.","full_name":"Endres, Robert G."},{"full_name":"Miodownik, Mark","first_name":"Mark","last_name":"Miodownik"},{"last_name":"Stramer","first_name":"Brian M.","full_name":"Stramer, Brian M."}]},{"language":[{"iso":"eng"}],"oa":1,"issue":"8","date_updated":"2021-01-12T08:13:47Z","oa_version":"Submitted Version","day":"01","status":"public","date_published":"2019-08-01T00:00:00Z","publication_status":"published","abstract":[{"lang":"eng","text":"The sebaceous gland (SG) is an essential component of the skin, and SG dysfunction is debilitating1,2. Yet, the cellular bases for its origin, development and subsequent maintenance remain poorly understood. Here, we apply large-scale quantitative fate mapping to define the patterns of cell fate behaviour during SG development and maintenance. We show that the SG develops from a defined number of lineage-restricted progenitors that undergo a programme of independent and stochastic cell fate decisions. Following an expansion phase, equipotent progenitors transition into a phase of homeostatic turnover, which is correlated with changes in the mechanical properties of the stroma and spatial restrictions on gland size. Expression of the oncogene KrasG12D results in a release from these constraints and unbridled gland expansion. Quantitative clonal fate analysis reveals that, during this phase, the primary effect of the Kras oncogene is to drive a constant fate bias with little effect on cell division rates. These findings provide insight into the developmental programme of the SG, as well as the mechanisms that drive tumour progression and gland dysfunction."}],"extern":"1","pmid":1,"doi":"10.1038/s41556-019-0362-x","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978139/","open_access":"1"}],"month":"08","author":[{"full_name":"Andersen, Marianne Stemann","last_name":"Andersen","first_name":"Marianne Stemann"},{"full_name":"Hannezo, Edouard B","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ulyanchenko, Svetlana","first_name":"Svetlana","last_name":"Ulyanchenko"},{"full_name":"Estrach, Soline","first_name":"Soline","last_name":"Estrach"},{"last_name":"Antoku","first_name":"Yasuko","full_name":"Antoku, Yasuko"},{"first_name":"Sabrina","last_name":"Pisano","full_name":"Pisano, Sabrina"},{"full_name":"Boonekamp, Kim E.","last_name":"Boonekamp","first_name":"Kim E."},{"last_name":"Sendrup","first_name":"Sarah","full_name":"Sendrup, Sarah"},{"last_name":"Maimets","first_name":"Martti","full_name":"Maimets, Martti"},{"full_name":"Pedersen, Marianne Terndrup","first_name":"Marianne Terndrup","last_name":"Pedersen"},{"full_name":"Johansen, Jens V.","last_name":"Johansen","first_name":"Jens V."},{"first_name":"Ditte L.","last_name":"Clement","full_name":"Clement, Ditte L."},{"last_name":"Feral","first_name":"Chloe C.","full_name":"Feral, Chloe C."},{"full_name":"Simons, Benjamin D.","first_name":"Benjamin D.","last_name":"Simons"},{"last_name":"Jensen","first_name":"Kim B.","full_name":"Jensen, Kim B."}],"_id":"7476","citation":{"apa":"Andersen, M. S., Hannezo, E. B., Ulyanchenko, S., Estrach, S., Antoku, Y., Pisano, S., … Jensen, K. B. (2019). Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41556-019-0362-x\">https://doi.org/10.1038/s41556-019-0362-x</a>","short":"M.S. Andersen, E.B. Hannezo, S. Ulyanchenko, S. Estrach, Y. Antoku, S. Pisano, K.E. Boonekamp, S. Sendrup, M. Maimets, M.T. Pedersen, J.V. Johansen, D.L. Clement, C.C. Feral, B.D. Simons, K.B. Jensen, Nature Cell Biology 21 (2019) 924–932.","chicago":"Andersen, Marianne Stemann, Edouard B Hannezo, Svetlana Ulyanchenko, Soline Estrach, Yasuko Antoku, Sabrina Pisano, Kim E. Boonekamp, et al. “Tracing the Cellular Dynamics of Sebaceous Gland Development in Normal and Perturbed States.” <i>Nature Cell Biology</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41556-019-0362-x\">https://doi.org/10.1038/s41556-019-0362-x</a>.","ieee":"M. S. Andersen <i>et al.</i>, “Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states,” <i>Nature Cell Biology</i>, vol. 21, no. 8. Springer Nature, pp. 924–932, 2019.","mla":"Andersen, Marianne Stemann, et al. “Tracing the Cellular Dynamics of Sebaceous Gland Development in Normal and Perturbed States.” <i>Nature Cell Biology</i>, vol. 21, no. 8, Springer Nature, 2019, pp. 924–32, doi:<a href=\"https://doi.org/10.1038/s41556-019-0362-x\">10.1038/s41556-019-0362-x</a>.","ista":"Andersen MS, Hannezo EB, Ulyanchenko S, Estrach S, Antoku Y, Pisano S, Boonekamp KE, Sendrup S, Maimets M, Pedersen MT, Johansen JV, Clement DL, Feral CC, Simons BD, Jensen KB. 2019. Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. Nature Cell Biology. 21(8), 924–932.","ama":"Andersen MS, Hannezo EB, Ulyanchenko S, et al. Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states. <i>Nature Cell Biology</i>. 2019;21(8):924-932. doi:<a href=\"https://doi.org/10.1038/s41556-019-0362-x\">10.1038/s41556-019-0362-x</a>"},"quality_controlled":"1","type":"journal_article","publisher":"Springer Nature","intvolume":"        21","year":"2019","publication":"Nature Cell Biology","external_id":{"pmid":["31358966"]},"article_type":"original","title":"Tracing the cellular dynamics of sebaceous gland development in normal and perturbed states","volume":21,"page":"924-932","publication_identifier":{"issn":["1465-7392","1476-4679"]},"date_created":"2020-02-11T08:43:49Z","article_processing_charge":"No"},{"month":"05","doi":"10.1038/ncb3524","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication_status":"published","abstract":[{"lang":"eng","text":"The seminal observation that mechanical signals can elicit changes in biochemical signalling within cells, a process commonly termed mechanosensation and mechanotransduction, has revolutionized our understanding of the role of cell mechanics in various fundamental biological processes, such as cell motility, adhesion, proliferation and differentiation. In this Review, we will discuss how the interplay and feedback between mechanical and biochemical signals control tissue morphogenesis and cell fate specification in embryonic development."}],"status":"public","date_published":"2017-05-31T00:00:00Z","day":"31","corr_author":"1","issue":"6","oa_version":"None","date_updated":"2025-09-10T14:23:21Z","language":[{"iso":"eng"}],"publication_identifier":{"issn":["1465-7392"]},"date_created":"2018-12-11T11:47:53Z","article_processing_charge":"No","department":[{"_id":"CaHe"}],"page":"581 - 588","publist_id":"7040","year":"2017","publication":"Nature Cell Biology","project":[{"name":"The generation and function of anisotropic tissue tension in zebrafish epiboly","_id":"25236028-B435-11E9-9278-68D0E5697425","grant_number":"ALTF534-2016"}],"external_id":{"isi":["000402525200003"]},"title":"Multiscale force sensing in development","volume":19,"publisher":"Nature Publishing Group","intvolume":"        19","type":"journal_article","isi":1,"citation":{"chicago":"Petridou, Nicoletta, Zoltan P Spiro, and Carl-Philipp J Heisenberg. “Multiscale Force Sensing in Development.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/ncb3524\">https://doi.org/10.1038/ncb3524</a>.","apa":"Petridou, N., Spiro, Z. P., &#38; Heisenberg, C.-P. J. (2017). Multiscale force sensing in development. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb3524\">https://doi.org/10.1038/ncb3524</a>","short":"N. Petridou, Z.P. Spiro, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 581–588.","ama":"Petridou N, Spiro ZP, Heisenberg C-PJ. Multiscale force sensing in development. <i>Nature Cell Biology</i>. 2017;19(6):581-588. doi:<a href=\"https://doi.org/10.1038/ncb3524\">10.1038/ncb3524</a>","mla":"Petridou, Nicoletta, et al. “Multiscale Force Sensing in Development.” <i>Nature Cell Biology</i>, vol. 19, no. 6, Nature Publishing Group, 2017, pp. 581–88, doi:<a href=\"https://doi.org/10.1038/ncb3524\">10.1038/ncb3524</a>.","ieee":"N. Petridou, Z. P. Spiro, and C.-P. J. Heisenberg, “Multiscale force sensing in development,” <i>Nature Cell Biology</i>, vol. 19, no. 6. Nature Publishing Group, pp. 581–588, 2017.","ista":"Petridou N, Spiro ZP, Heisenberg C-PJ. 2017. Multiscale force sensing in development. Nature Cell Biology. 19(6), 581–588."},"quality_controlled":"1","scopus_import":"1","author":[{"full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","last_name":"Petridou","first_name":"Nicoletta"},{"full_name":"Spiro, Zoltan P","first_name":"Zoltan P","id":"426AD026-F248-11E8-B48F-1D18A9856A87","last_name":"Spiro"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"_id":"678"},{"author":[{"full_name":"Smutny, Michael","first_name":"Michael","orcid":"0000-0002-5920-9090","last_name":"Smutny","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Zsuzsa","last_name":"Ákos","full_name":"Ákos, Zsuzsa"},{"first_name":"Silvia","last_name":"Grigolon","full_name":"Grigolon, Silvia"},{"first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","last_name":"Shamipour","full_name":"Shamipour, Shayan"},{"first_name":"Verena","last_name":"Ruprecht","full_name":"Ruprecht, Verena"},{"full_name":"Capek, Daniel","first_name":"Daniel","last_name":"Capek","id":"31C42484-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5199-9940"},{"full_name":"Behrndt, Martin","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt"},{"first_name":"Ekaterina","last_name":"Papusheva","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","full_name":"Papusheva, Ekaterina"},{"full_name":"Tada, Masazumi","last_name":"Tada","first_name":"Masazumi"},{"full_name":"Hof, Björn","id":"3A374330-F248-11E8-B48F-1D18A9856A87","last_name":"Hof","orcid":"0000-0003-2057-2754","first_name":"Björn"},{"full_name":"Vicsek, Tamás","first_name":"Tamás","last_name":"Vicsek"},{"last_name":"Salbreux","first_name":"Guillaume","full_name":"Salbreux, Guillaume"},{"orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"_id":"661","citation":{"mla":"Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” <i>Nature Cell Biology</i>, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:<a href=\"https://doi.org/10.1038/ncb3492\">10.1038/ncb3492</a>.","ieee":"M. Smutny <i>et al.</i>, “Friction forces position the neural anlage,” <i>Nature Cell Biology</i>, vol. 19. Nature Publishing Group, pp. 306–317, 2017.","ista":"Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M, Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction forces position the neural anlage. Nature Cell Biology. 19, 306–317.","ama":"Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage. <i>Nature Cell Biology</i>. 2017;19:306-317. doi:<a href=\"https://doi.org/10.1038/ncb3492\">10.1038/ncb3492</a>","short":"M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M. Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 306–317.","apa":"Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D., … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb3492\">https://doi.org/10.1038/ncb3492</a>","chicago":"Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural Anlage.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/ncb3492\">https://doi.org/10.1038/ncb3492</a>."},"scopus_import":"1","quality_controlled":"1","type":"journal_article","isi":1,"publisher":"Nature Publishing Group","intvolume":"        19","year":"2017","project":[{"_id":"25152F3A-B435-11E9-9278-68D0E5697425","name":"Decoding the complexity of turbulence at its origin","grant_number":"306589","call_identifier":"FP7"},{"_id":"252ABD0A-B435-11E9-9278-68D0E5697425","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","grant_number":"I930-B20","call_identifier":"FWF"}],"publication":"Nature Cell Biology","external_id":{"pmid":["28346437"],"isi":["000397917000009"]},"title":"Friction forces position the neural anlage","volume":19,"related_material":{"record":[{"status":"public","id":"8350","relation":"dissertation_contains"},{"relation":"dissertation_contains","id":"50","status":"public"}]},"page":"306 - 317","publist_id":"7074","department":[{"_id":"CaHe"},{"_id":"BjHo"},{"_id":"Bio"}],"acknowledged_ssus":[{"_id":"SSU"}],"publication_identifier":{"issn":["1465-7392"]},"date_created":"2018-12-11T11:47:46Z","article_processing_charge":"No","language":[{"iso":"eng"}],"oa":1,"ec_funded":1,"oa_version":"Submitted Version","date_updated":"2026-05-02T22:31:03Z","corr_author":"1","day":"27","status":"public","date_published":"2017-03-27T00:00:00Z","publication_status":"published","abstract":[{"lang":"eng","text":"During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo."}],"pmid":1,"doi":"10.1038/ncb3492","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","main_file_link":[{"url":"https://europepmc.org/articles/pmc5635970","open_access":"1"}],"month":"03"},{"publication_identifier":{"eissn":["1476-4679"],"issn":["1465-7392"]},"article_processing_charge":"No","date_created":"2022-04-07T07:56:04Z","publication":"Nature Cell Biology","year":"2007","volume":9,"title":"Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum","article_type":"original","external_id":{"pmid":["17828249"]},"page":"1160-1166","type":"journal_article","publisher":"Springer Nature","intvolume":"         9","_id":"11115","author":[{"full_name":"Anderson, Daniel J.","first_name":"Daniel J.","last_name":"Anderson"},{"full_name":"HETZER, Martin W","first_name":"Martin W","orcid":"0000-0002-2111-992X","last_name":"HETZER","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed"}],"keyword":["Cell Biology"],"citation":{"short":"D.J. Anderson, M. Hetzer, Nature Cell Biology 9 (2007) 1160–1166.","apa":"Anderson, D. J., &#38; Hetzer, M. (2007). Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/ncb1636\">https://doi.org/10.1038/ncb1636</a>","chicago":"Anderson, Daniel J., and Martin Hetzer. “Nuclear Envelope Formation by Chromatin-Mediated Reorganization of the Endoplasmic Reticulum.” <i>Nature Cell Biology</i>. Springer Nature, 2007. <a href=\"https://doi.org/10.1038/ncb1636\">https://doi.org/10.1038/ncb1636</a>.","ieee":"D. J. Anderson and M. Hetzer, “Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum,” <i>Nature Cell Biology</i>, vol. 9, no. 10. Springer Nature, pp. 1160–1166, 2007.","ista":"Anderson DJ, Hetzer M. 2007. Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. Nature Cell Biology. 9(10), 1160–1166.","mla":"Anderson, Daniel J., and Martin Hetzer. “Nuclear Envelope Formation by Chromatin-Mediated Reorganization of the Endoplasmic Reticulum.” <i>Nature Cell Biology</i>, vol. 9, no. 10, Springer Nature, 2007, pp. 1160–66, doi:<a href=\"https://doi.org/10.1038/ncb1636\">10.1038/ncb1636</a>.","ama":"Anderson DJ, Hetzer M. Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum. <i>Nature Cell Biology</i>. 2007;9(10):1160-1166. doi:<a href=\"https://doi.org/10.1038/ncb1636\">10.1038/ncb1636</a>"},"scopus_import":"1","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"doi":"10.1038/ncb1636","month":"09","status":"public","date_published":"2007-09-09T00:00:00Z","extern":"1","publication_status":"published","abstract":[{"lang":"eng","text":"The formation of the nuclear envelope (NE) around chromatin is a major membrane-remodelling event that occurs during cell division of metazoa. It is unclear whether the nuclear membrane reforms by the fusion of NE fragments or if it re-emerges from an intact tubular network of the endoplasmic reticulum (ER). Here, we show that NE formation and expansion requires a tubular ER network and occurs efficiently in the presence of the membrane fusion inhibitor GTPγS. Chromatin recruitment of membranes, which is initiated by tubule-end binding, followed by the formation, expansion and sealing of flat membrane sheets, is mediated by DNA-binding proteins residing in the ER. Thus, chromatin plays an active role in reshaping of the ER during NE formation."}],"day":"09","language":[{"iso":"eng"}],"date_updated":"2024-10-14T11:30:08Z","oa_version":"None","issue":"10"},{"page":"E177-E184","volume":4,"title":"The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly","article_type":"original","external_id":{"pmid":["12105431"]},"publication":"Nature Cell Biology","year":"2002","article_processing_charge":"No","date_created":"2022-04-07T07:57:19Z","publication_identifier":{"issn":["1465-7392"],"eissn":["1476-4679"]},"quality_controlled":"1","scopus_import":"1","keyword":["Cell Biology"],"citation":{"mla":"Hetzer, Martin, et al. “The Ran GTPase as a Marker of Chromosome Position in Spindle Formation and Nuclear Envelope Assembly.” <i>Nature Cell Biology</i>, vol. 4, no. 7, Springer Nature, 2002, pp. E177–84, doi:<a href=\"https://doi.org/10.1038/ncb0702-e177\">10.1038/ncb0702-e177</a>.","ista":"Hetzer M, Gruss OJ, Mattaj IW. 2002. The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly. Nature Cell Biology. 4(7), E177–E184.","ieee":"M. Hetzer, O. J. Gruss, and I. W. Mattaj, “The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly,” <i>Nature Cell Biology</i>, vol. 4, no. 7. Springer Nature, pp. E177–E184, 2002.","ama":"Hetzer M, Gruss OJ, Mattaj IW. The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly. <i>Nature Cell Biology</i>. 2002;4(7):E177-E184. doi:<a href=\"https://doi.org/10.1038/ncb0702-e177\">10.1038/ncb0702-e177</a>","short":"M. Hetzer, O.J. Gruss, I.W. Mattaj, Nature Cell Biology 4 (2002) E177–E184.","apa":"Hetzer, M., Gruss, O. J., &#38; Mattaj, I. W. (2002). The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/ncb0702-e177\">https://doi.org/10.1038/ncb0702-e177</a>","chicago":"Hetzer, Martin, Oliver J. Gruss, and Iain W. Mattaj. “The Ran GTPase as a Marker of Chromosome Position in Spindle Formation and Nuclear Envelope Assembly.” <i>Nature Cell Biology</i>. Springer Nature, 2002. <a href=\"https://doi.org/10.1038/ncb0702-e177\">https://doi.org/10.1038/ncb0702-e177</a>."},"_id":"11123","author":[{"first_name":"Martin W","orcid":"0000-0002-2111-992X","last_name":"HETZER","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","full_name":"HETZER, Martin W"},{"first_name":"Oliver J.","last_name":"Gruss","full_name":"Gruss, Oliver J."},{"full_name":"Mattaj, Iain W.","last_name":"Mattaj","first_name":"Iain W."}],"intvolume":"         4","publisher":"Springer Nature","type":"journal_article","extern":"1","publication_status":"published","abstract":[{"text":"The small GTPase Ran is a key regulator of nucleocytoplasmic transport during interphase. The asymmetric distribution of the GTP-bound form of Ran across the nuclear envelope — that is, large quantities in the nucleus compared with small quantities in the cytoplasm — determines the directionality of many nuclear transport processes. Recent findings that Ran also functions in spindle formation and nuclear envelope assembly during mitosis suggest that Ran has a general role in chromatin-centred processes. Ran functions in these events as a signal for chromosome position.","lang":"eng"}],"date_published":"2002-07-01T00:00:00Z","status":"public","month":"07","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","doi":"10.1038/ncb0702-e177","pmid":1,"date_updated":"2022-07-18T08:58:03Z","oa_version":"None","issue":"7","language":[{"iso":"eng"}],"day":"01"},{"type":"journal_article","publisher":"Springer Nature","intvolume":"         3","_id":"11125","author":[{"orcid":"0000-0002-2111-992X","last_name":"HETZER","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","first_name":"Martin W","full_name":"HETZER, Martin W"},{"first_name":"Hemmo H.","last_name":"Meyer","full_name":"Meyer, Hemmo H."},{"full_name":"Walther, Tobias C.","first_name":"Tobias C.","last_name":"Walther"},{"first_name":"Daniel","last_name":"Bilbao-Cortes","full_name":"Bilbao-Cortes, Daniel"},{"full_name":"Warren, Graham","first_name":"Graham","last_name":"Warren"},{"first_name":"Iain W.","last_name":"Mattaj","full_name":"Mattaj, Iain W."}],"keyword":["Cell Biology"],"citation":{"short":"M. Hetzer, H.H. Meyer, T.C. Walther, D. Bilbao-Cortes, G. Warren, I.W. Mattaj, Nature Cell Biology 3 (2001) 1086–1091.","apa":"Hetzer, M., Meyer, H. H., Walther, T. C., Bilbao-Cortes, D., Warren, G., &#38; Mattaj, I. W. (2001). Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. <i>Nature Cell Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/ncb1201-1086\">https://doi.org/10.1038/ncb1201-1086</a>","chicago":"Hetzer, Martin, Hemmo H. Meyer, Tobias C. Walther, Daniel Bilbao-Cortes, Graham Warren, and Iain W. Mattaj. “Distinct AAA-ATPase P97 Complexes Function in Discrete Steps of Nuclear Assembly.” <i>Nature Cell Biology</i>. Springer Nature, 2001. <a href=\"https://doi.org/10.1038/ncb1201-1086\">https://doi.org/10.1038/ncb1201-1086</a>.","ieee":"M. Hetzer, H. H. Meyer, T. C. Walther, D. Bilbao-Cortes, G. Warren, and I. W. Mattaj, “Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly,” <i>Nature Cell Biology</i>, vol. 3, no. 12. Springer Nature, pp. 1086–1091, 2001.","mla":"Hetzer, Martin, et al. “Distinct AAA-ATPase P97 Complexes Function in Discrete Steps of Nuclear Assembly.” <i>Nature Cell Biology</i>, vol. 3, no. 12, Springer Nature, 2001, pp. 1086–91, doi:<a href=\"https://doi.org/10.1038/ncb1201-1086\">10.1038/ncb1201-1086</a>.","ista":"Hetzer M, Meyer HH, Walther TC, Bilbao-Cortes D, Warren G, Mattaj IW. 2001. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. Nature Cell Biology. 3(12), 1086–1091.","ama":"Hetzer M, Meyer HH, Walther TC, Bilbao-Cortes D, Warren G, Mattaj IW. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. <i>Nature Cell Biology</i>. 2001;3(12):1086-1091. doi:<a href=\"https://doi.org/10.1038/ncb1201-1086\">10.1038/ncb1201-1086</a>"},"quality_controlled":"1","scopus_import":"1","publication_identifier":{"issn":["1465-7392"],"eissn":["1476-4679"]},"article_processing_charge":"No","date_created":"2022-04-07T07:57:42Z","publication":"Nature Cell Biology","year":"2001","volume":3,"title":"Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly","external_id":{"pmid":["11781570"]},"article_type":"original","page":"1086-1091","day":"02","language":[{"iso":"eng"}],"oa_version":"None","date_updated":"2022-07-18T08:58:07Z","issue":"12","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","pmid":1,"doi":"10.1038/ncb1201-1086","month":"11","status":"public","date_published":"2001-11-02T00:00:00Z","extern":"1","publication_status":"published","abstract":[{"text":"Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97–Ufd1–Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97–p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation.","lang":"eng"}]}]