[{"article_type":"original","external_id":{"pmid":["41043435"]},"status":"public","date_published":"2025-10-02T00:00:00Z","date_created":"2025-11-12T10:03:39Z","article_processing_charge":"No","OA_type":"closed access","page":"S1534-5807(25)00569-6","abstract":[{"lang":"eng","text":"The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis."}],"author":[{"last_name":"Huang","first_name":"R","full_name":"Huang, R"},{"last_name":"Wang","full_name":"Wang, J","first_name":"J"},{"last_name":"Chang","full_name":"Chang, M","first_name":"M"},{"full_name":"Tang, W","first_name":"W","last_name":"Tang"},{"last_name":"Yu","first_name":"Y","full_name":"Yu, Y"},{"last_name":"Zhang","full_name":"Zhang, Y","first_name":"Y"},{"last_name":"Peng","first_name":"Y","full_name":"Peng, Y"},{"first_name":"Y","full_name":"Wang, Y","last_name":"Wang"},{"last_name":"Guo","first_name":"Y","full_name":"Guo, Y"},{"full_name":"Lu, T","first_name":"T","last_name":"Lu"},{"last_name":"Cao","first_name":"Y","full_name":"Cao, Y"},{"first_name":"Y","full_name":"Zhou, Y","last_name":"Zhou"},{"last_name":"Zhang","first_name":"Q","full_name":"Zhang, Q"},{"first_name":"Y","full_name":"Huang, Y","last_name":"Huang"},{"last_name":"Wu","full_name":"Wu, A","first_name":"A"},{"last_name":"Ren","full_name":"Ren, L","first_name":"L"},{"last_name":"Gallei","id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368"},{"full_name":"Dong, J","first_name":"J","last_name":"Dong"},{"full_name":"Chen, H","first_name":"H","last_name":"Chen"},{"last_name":"He","first_name":"J","full_name":"He, J"},{"last_name":"Wen","full_name":"Wen, M","first_name":"M"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"},{"first_name":"L","full_name":"Sun, L","last_name":"Sun"},{"last_name":"Xiong","full_name":"Xiong, Y","first_name":"Y"},{"last_name":"Yang","full_name":"Yang, Z","first_name":"Z"},{"last_name":"Xu","first_name":"T","full_name":"Xu, T"}],"quality_controlled":"1","acknowledgement":"We thank Lukáš Fiedler for helping with the writing. This work was supported by the National Key Research and Development Program of China (2023YFA0913500) to T.X., R.H., Y.Y., Y.X., and M.W. and by the National Natural Science Foundation of China grants to T.X. (32130010), Z.Y. (3241101698), and R.H. (32070309 and 32470276) and startup funds from the Fujian Agriculture and Forestry University and the Shanghai Plant Stress Biology Center, Chinese Academy of Sciences to T.X.","year":"2025","language":[{"iso":"eng"}],"publication":"Developmental Cell","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"department":[{"_id":"JiFr"}],"publication_status":"epub_ahead","citation":{"apa":"Huang, R., Wang, J., Chang, M., Tang, W., Yu, Y., Zhang, Y., … Xu, T. (2025). TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.09.009","ista":"Huang R, Wang J, Chang M, Tang W, Yu Y, Zhang Y, Peng Y, Wang Y, Guo Y, Lu T, Cao Y, Zhou Y, Zhang Q, Huang Y, Wu A, Ren L, Gallei MC, Dong J, Chen H, He J, Wen M, Friml J, Sun L, Xiong Y, Yang Z, Xu T. 2025. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell., S1534-5807(25)00569–6.","ieee":"R. Huang et al., “TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis,” Developmental Cell. Elsevier, pp. S1534-5807(25)00569–6, 2025.","mla":"Huang, R., et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” Developmental Cell, Elsevier, 2025, pp. S1534-5807(25)00569-6, doi:10.1016/j.devcel.2025.09.009.","chicago":"Huang, R, J Wang, M Chang, W Tang, Y Yu, Y Zhang, Y Peng, et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2025.09.009.","short":"R. Huang, J. Wang, M. Chang, W. Tang, Y. Yu, Y. Zhang, Y. Peng, Y. Wang, Y. Guo, T. Lu, Y. Cao, Y. Zhou, Q. Zhang, Y. Huang, A. Wu, L. Ren, M.C. Gallei, J. Dong, H. Chen, J. He, M. Wen, J. Friml, L. Sun, Y. Xiong, Z. Yang, T. Xu, Developmental Cell (2025) S1534-5807(25)00569–6.","ama":"Huang R, Wang J, Chang M, et al. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. 2025:S1534-5807(25)00569-6. doi:10.1016/j.devcel.2025.09.009"},"publisher":"Elsevier","doi":"10.1016/j.devcel.2025.09.009","day":"02","oa_version":"None","scopus_import":"1","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","title":"TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis","_id":"20636","date_updated":"2025-11-24T13:43:08Z","month":"10"},{"pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","date_updated":"2025-09-30T12:07:36Z","_id":"19594","month":"04","title":"Unlocking plant regeneration: The role for glutathione","corr_author":"1","citation":{"chicago":"Benková, Eva. “Unlocking Plant Regeneration: The Role for Glutathione.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2025.03.012.","short":"E. Benková, Developmental Cell 60 (2025) 1137–1139.","ama":"Benková E. Unlocking plant regeneration: The role for glutathione. Developmental Cell. 2025;60(8):1137-1139. doi:10.1016/j.devcel.2025.03.012","mla":"Benková, Eva. “Unlocking Plant Regeneration: The Role for Glutathione.” Developmental Cell, vol. 60, no. 8, Elsevier, 2025, pp. 1137–39, doi:10.1016/j.devcel.2025.03.012.","ista":"Benková E. 2025. Unlocking plant regeneration: The role for glutathione. Developmental Cell. 60(8), 1137–1139.","apa":"Benková, E. (2025). Unlocking plant regeneration: The role for glutathione. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.03.012","ieee":"E. Benková, “Unlocking plant regeneration: The role for glutathione,” Developmental Cell, vol. 60, no. 8. Elsevier, pp. 1137–1139, 2025."},"publisher":"Elsevier","publication_status":"published","department":[{"_id":"EvBe"}],"intvolume":" 60","scopus_import":"1","volume":60,"oa_version":"None","day":"21","doi":"10.1016/j.devcel.2025.03.012","issue":"8","author":[{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková"}],"quality_controlled":"1","isi":1,"language":[{"iso":"eng"}],"publication":"Developmental Cell","year":"2025","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"status":"public","article_processing_charge":"No","date_published":"2025-04-21T00:00:00Z","date_created":"2025-04-20T22:01:28Z","external_id":{"pmid":["40262524"],"isi":["001477400800001"]},"article_type":"letter_note","abstract":[{"lang":"eng","text":"In this issue of Developmental Cell, Lee et al. identify a pivotal role for glutathione (GSH) in plant regeneration, a vital biological process enabling plants to regrow tissues and organs after injury. Applying single-cell RNA sequencing (scRNA-seq) and live imaging, the authors demonstrate that GSH, released upon tissue damage, accelerates cell-cycle transitions, particularly shortening the G1 phase, thereby facilitating efficient organ regeneration."}],"page":"1137-1139","OA_type":"closed access"},{"language":[{"iso":"eng"}],"publication":"Developmental Cell","year":"2025","isi":1,"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"acknowledgement":"We thank A. Dimitracopoulos, K. Kawaguchi, J. Vidigueira, B. Baum, I. McLaren, D. St Johnston, and members of the Buckley, Scarpa, Steventon, Kawaguchi, and Xiong labs for technical assistance and constructive feedback. We thank Ryan Greenhalgh for methods developed to obtain fluidity values from AFM data. We thank Nicola Lawrence, Alex Sossick, and Sargon Gross-Thebing from the Gurdon Institute Imaging Facility for microscopy support. Funding: this work was supported by a Wellcome Trust/Royal Society Sir Henry Dale Fellowship (215439/Z/19/Z) and UKRI-EPSRC Frontier Research Grant (EP/X023761/1, originally selected as an ERC Starting Grant) to F.X.; an ERC Consolidator Grant (772426), ERC Synergy Grant 101118729 UNFOLD, and Alexander von Humboldt Professorship ( Alexander von Humboldt Foundation) to K.F.; and an ERC Starting Grant (851288) to E.H.","file_date_updated":"2025-12-29T13:45:05Z","issue":"17","author":[{"last_name":"Mclaren","full_name":"Mclaren, Susannah B.P.","first_name":"Susannah B.P."},{"last_name":"Xue","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","first_name":"Shi-lei","full_name":"Xue, Shi-lei"},{"last_name":"Ding","first_name":"Siyuan","full_name":"Ding, Siyuan"},{"full_name":"Winkel, Alexander K.","first_name":"Alexander K.","last_name":"Winkel"},{"last_name":"Baldwin","first_name":"Oscar","full_name":"Baldwin, Oscar"},{"last_name":"Dwarakacherla","full_name":"Dwarakacherla, Shreya","first_name":"Shreya"},{"first_name":"Kristian","full_name":"Franze, Kristian","last_name":"Franze"},{"last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"full_name":"Xiong, Fengzhu","first_name":"Fengzhu","last_name":"Xiong"}],"quality_controlled":"1","PlanS_conform":"1","abstract":[{"text":"An enlarged brain underlies the complex central nervous system of vertebrates. The dramatic expansion of the brain that diverges its shape from the spinal cord follows neural tube closure during embryonic development. Here, we show that this differential deformation is encoded by a pre-pattern of tissue material properties in chicken embryos. Using magnetic droplets and atomic force microscopy, we demonstrate that the dorsal hindbrain is more fluid than the dorsal spinal cord, resulting in a thinning versus a resisting response to increasing lumen pressure, respectively. The dorsal hindbrain exhibits reduced apical actin and a disorganized laminin matrix consistent with tissue fluidization. Blocking the activity of neural-crest-associated matrix metalloproteinases inhibits hindbrain expansion. Transplanting dorsal hindbrain cells to the spinal cord can locally create an expanded brain-like morphology in some cases. Our findings raise questions in vertebrate head evolution and suggest a general role of mechanical pre-patterning in sculpting epithelial tubes.","lang":"eng"}],"page":"2237-2247.e4","OA_type":"hybrid","file":[{"relation":"main_file","date_created":"2025-12-29T13:45:05Z","checksum":"1ca6f0822c1cbd430686d5e2a4f96401","file_name":"2025_DevelopmentalCell_McLaren.pdf","content_type":"application/pdf","success":1,"access_level":"open_access","creator":"dernst","file_id":"20872","date_updated":"2025-12-29T13:45:05Z","file_size":12564806}],"OA_place":"publisher","status":"public","article_processing_charge":"Yes (in subscription journal)","date_published":"2025-09-08T00:00:00Z","date_created":"2025-05-18T22:02:50Z","external_id":{"pmid":["40347948"],"isi":["001570502100005"]},"license":"https://creativecommons.org/licenses/by/4.0/","article_type":"original","date_updated":"2025-12-29T14:58:14Z","_id":"19703","month":"09","title":"Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord","ddc":["570"],"pmid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","ec_funded":1,"volume":60,"has_accepted_license":"1","scopus_import":"1","project":[{"grant_number":"851288","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}],"day":"08","oa_version":"Published Version","doi":"10.1016/j.devcel.2025.04.010","oa":1,"citation":{"chicago":"Mclaren, Susannah B.P., Shi-lei Xue, Siyuan Ding, Alexander K. Winkel, Oscar Baldwin, Shreya Dwarakacherla, Kristian Franze, Edouard B Hannezo, and Fengzhu Xiong. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2025.04.010.","ama":"Mclaren SBP, Xue S, Ding S, et al. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. Developmental Cell. 2025;60(17):2237-2247.e4. doi:10.1016/j.devcel.2025.04.010","short":"S.B.P. Mclaren, S. Xue, S. Ding, A.K. Winkel, O. Baldwin, S. Dwarakacherla, K. Franze, E.B. Hannezo, F. Xiong, Developmental Cell 60 (2025) 2237–2247.e4.","mla":"Mclaren, Susannah B. P., et al. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” Developmental Cell, vol. 60, no. 17, Elsevier, 2025, p. 2237–2247.e4, doi:10.1016/j.devcel.2025.04.010.","apa":"Mclaren, S. B. P., Xue, S., Ding, S., Winkel, A. K., Baldwin, O., Dwarakacherla, S., … Xiong, F. (2025). Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.04.010","ieee":"S. B. P. Mclaren et al., “Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord,” Developmental Cell, vol. 60, no. 17. Elsevier, p. 2237–2247.e4, 2025.","ista":"Mclaren SBP, Xue S, Ding S, Winkel AK, Baldwin O, Dwarakacherla S, Franze K, Hannezo EB, Xiong F. 2025. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. Developmental Cell. 60(17), 2237–2247.e4."},"publisher":"Elsevier","publication_status":"published","department":[{"_id":"EdHa"}],"intvolume":" 60"},{"volume":60,"has_accepted_license":"1","scopus_import":"1","project":[{"_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","grant_number":"FTI21-D-046"},{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity"}],"day":"10","oa_version":"Published Version","doi":"10.1016/j.devcel.2024.10.025","oa":1,"citation":{"ieee":"E. C. B. Jaeger et al., “Adeno-associated viral tools to trace neural development and connectivity across amphibians,” Developmental Cell, vol. 60, no. 5. Elsevier, p. 794–812.e6, 2025.","ista":"Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ, Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA, Sweeney LB. 2025. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. 60(5), 794–812.e6.","apa":"Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian, G., … Sweeney, L. B. (2025). Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2024.10.025","mla":"Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” Developmental Cell, vol. 60, no. 5, Elsevier, 2025, p. 794–812.e6, doi:10.1016/j.devcel.2024.10.025.","short":"E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian, J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma, G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M. Shein-Idelson, M.A. Tosches, L.B. Sweeney, Developmental Cell 60 (2025) 794–812.e6.","ama":"Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. 2025;60(5):794-812.e6. doi:10.1016/j.devcel.2024.10.025","chicago":"Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2024.10.025."},"publisher":"Elsevier","publication_status":"published","department":[{"_id":"LoSw"},{"_id":"MaDe"},{"_id":"GaNo"}],"intvolume":" 60","date_updated":"2025-09-30T10:00:55Z","_id":"15016","month":"03","ddc":["570"],"title":"Adeno-associated viral tools to trace neural development and connectivity across amphibians","corr_author":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","abstract":[{"lang":"eng","text":"Amphibians, by virtue of their phylogenetic position, provide invaluable insights on nervous system evolution, development, and remodeling. The genetic toolkit for amphibians, however, remains limited. Recombinant adeno-associated viral vectors (AAVs) are a powerful alternative to transgenesis for labeling and manipulating neurons. Although successful in mammals, AAVs have never been shown to transduce amphibian cells efficiently. We screened AAVs in three amphibian species—the frogs Xenopus laevis and Pelophylax bedriagae and the salamander Pleurodeles waltl—and identified at least two AAV serotypes per species that transduce neurons. In developing amphibians, AAVs labeled groups of neurons generated at the same time during development. In the mature brain, AAVrg retrogradely traced long-range projections. Our study introduces AAVs as a tool for amphibian research, establishes a generalizable workflow for AAV screening in new species, and expands opportunities for cross-species comparisons of nervous system development, function, and evolution."}],"page":"794-812.e6","OA_type":"hybrid","file":[{"date_created":"2025-06-04T05:43:27Z","file_name":"2025_DevelopmentalCell_Jaeger.pdf","checksum":"a83a4cb58f5941096d3ad91ca0172594","relation":"main_file","file_id":"19790","date_updated":"2025-06-04T05:43:27Z","file_size":11936258,"content_type":"application/pdf","success":1,"creator":"dernst","access_level":"open_access"}],"status":"public","OA_place":"publisher","article_processing_charge":"Yes (via OA deal)","date_created":"2024-02-20T09:20:32Z","date_published":"2025-03-10T00:00:00Z","external_id":{"isi":["001444798600001"],"pmid":["39603234"]},"article_type":"original","publication":"Developmental Cell","language":[{"iso":"eng"}],"isi":1,"year":"2025","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"file_date_updated":"2025-06-04T05:43:27Z","acknowledgement":"We thank members of the Sweeney, Tosches, Shein-Idelson, Yamaguchi, Kelley, and Cline Labs for their contributions to this project, discussion, and support. We additionally thank the Beckman Institute CLOVER Center and Viviana Gradinaru (Caltech), Kimberly Ritola (UNC NeuroTools), and Flavia Gomez-Leite (ISTA Viral Core) for AAV production and consultation; Andras Simon and Alberto Joven (Karolinska Institute) for feedback; Elizabeth Bagnato-Cohen (Columbia) for project coordination; our animal care and imaging facilities; the amphibian stock centers (NXR, EXRC, and XenopusExpress); and our funding sources: NSF IOS 2110086 (D.B.K., L.B.S., M.A.T., A.Y., and H.T.C.); US-Israel Binational Science Foundation (BSF) 2020702 (M.S.-I.); FTI Strategy Lower Austria Dissertation FT121-D-046 (D.V.); Horizon Europe ERC Starting Grant 101041551 and Special Research Programme (SFB) of the Austrian Science Fund (FWF) project F7814-B (L.B.S.); NIH grant R35GM146973, Rita Allen Foundation Award GA_032522_FE, and CZI Ben Barres Early Career Acceleration Award 2023-331758 (M.A.T.); EMBO Long-Term Fellowship ALTF 874-2021 (A.D.); and NSF GRFP DGE 2036197 (E.C.B.J.).","author":[{"first_name":"Eliza C.B.","full_name":"Jaeger, Eliza C.B.","last_name":"Jaeger"},{"id":"cf391e77-ec3c-11ea-a124-d69323410b58","first_name":"David","full_name":"Vijatovic, David","last_name":"Vijatovic"},{"last_name":"Deryckere","full_name":"Deryckere, Astrid","first_name":"Astrid"},{"last_name":"Zorin","first_name":"Nikol","full_name":"Zorin, Nikol"},{"last_name":"Nguyen","first_name":"Akemi L.","full_name":"Nguyen, Akemi L."},{"last_name":"Ivanian","id":"eaf2b366-cfd1-11ee-bbdf-c8790f800a05","first_name":"Georgiy","full_name":"Ivanian, Georgiy"},{"last_name":"Woych","full_name":"Woych, Jamie","first_name":"Jamie"},{"last_name":"Arnold","full_name":"Arnold, Rebecca C","id":"d6cce458-14c9-11ed-a755-c1c8fc6fde6f","first_name":"Rebecca C"},{"last_name":"Ortega Gurrola","full_name":"Ortega Gurrola, Alonso","first_name":"Alonso"},{"full_name":"Shvartsman, Arik","first_name":"Arik","last_name":"Shvartsman"},{"last_name":"Barbieri","id":"a9492887-8972-11ed-ae7b-bfae10998254","first_name":"Francesca","full_name":"Barbieri, Francesca"},{"full_name":"Toma, Florina-Alexandra","id":"85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5","first_name":"Florina-Alexandra","last_name":"Toma"},{"first_name":"Gary J.","full_name":"Gorbsky, Gary J.","last_name":"Gorbsky"},{"first_name":"Marko E.","full_name":"Horb, Marko E.","last_name":"Horb"},{"full_name":"Cline, Hollis T.","first_name":"Hollis T.","last_name":"Cline"},{"last_name":"Shay","first_name":"Timothy F.","full_name":"Shay, Timothy F."},{"last_name":"Kelley","first_name":"Darcy B.","full_name":"Kelley, Darcy B."},{"last_name":"Yamaguchi","full_name":"Yamaguchi, Ayako","first_name":"Ayako"},{"last_name":"Shein-Idelson","full_name":"Shein-Idelson, Mark","first_name":"Mark"},{"first_name":"Maria Antonietta","full_name":"Tosches, Maria Antonietta","last_name":"Tosches"},{"last_name":"Sweeney","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601","full_name":"Sweeney, Lora Beatrice Jaeger"}],"issue":"5","quality_controlled":"1","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}]},{"month":"11","date_updated":"2025-12-29T09:23:58Z","_id":"20859","title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses","ddc":["570"],"type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","scopus_import":"1","oa_version":"Published Version","day":"04","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2025.10.006"}],"doi":"10.1016/j.devcel.2025.10.006","publisher":"Elsevier","oa":1,"citation":{"ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2025. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell.","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2025). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.10.006","ieee":"I. Company-Garrido et al., “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” Developmental Cell. Elsevier, 2025.","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” Developmental Cell, Elsevier, 2025, doi:10.1016/j.devcel.2025.10.006.","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 2025. doi:10.1016/j.devcel.2025.10.006","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell (2025).","chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2025.10.006."},"publication_status":"epub_ahead","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"language":[{"iso":"eng"}],"year":"2025","publication":"Developmental Cell","acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","quality_controlled":"1","author":[{"last_name":"Company-Garrido","first_name":"Iván","full_name":"Company-Garrido, Iván"},{"full_name":"Zurita Carpio, Alberto","first_name":"Alberto","last_name":"Zurita Carpio"},{"last_name":"Colomer-Rosell","first_name":"Mariona","full_name":"Colomer-Rosell, Mariona"},{"last_name":"Ciraulo","first_name":"Bernard","full_name":"Ciraulo, Bernard"},{"last_name":"Molkenbur","full_name":"Molkenbur, Ronja","first_name":"Ronja"},{"first_name":"Peter","full_name":"Lanzerstorfer, Peter","last_name":"Lanzerstorfer"},{"last_name":"Pezzano","full_name":"Pezzano, Fabio","first_name":"Fabio"},{"full_name":"Agazzi, Costanza","first_name":"Costanza","last_name":"Agazzi"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"last_name":"Jain","full_name":"Jain, Saumey","first_name":"Saumey"},{"first_name":"Jeroen M.","full_name":"Jacques, Jeroen M.","last_name":"Jacques"},{"last_name":"Venturini","first_name":"Valeria","full_name":"Venturini, Valeria"},{"last_name":"Knapp","first_name":"Christian","full_name":"Knapp, Christian"},{"last_name":"Xie","full_name":"Xie, Yufei","first_name":"Yufei"},{"last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"first_name":"Julian","full_name":"Weghuber, Julian","last_name":"Weghuber"},{"last_name":"Schaaf","full_name":"Schaaf, Marcel","first_name":"Marcel"},{"last_name":"Quidant","first_name":"Romain","full_name":"Quidant, Romain"},{"orcid":"0000-0001-6165-5738","full_name":"Kiermaier, Eva","first_name":"Eva","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","last_name":"Kiermaier"},{"first_name":"Jaime","full_name":"Ortega Arroyo, Jaime","last_name":"Ortega Arroyo"},{"last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan","orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan","last_name":"Wieser"}],"acknowledged_ssus":[{"_id":"NanoFab"}],"PlanS_conform":"1","abstract":[{"lang":"eng","text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo."}],"OA_type":"hybrid","article_processing_charge":"Yes (in subscription journal)","date_published":"2025-11-04T00:00:00Z","date_created":"2025-12-28T23:01:27Z","status":"public","OA_place":"publisher","external_id":{"pmid":["41192429"]},"article_type":"original"},{"publication_identifier":{"issn":["1534-5807"]},"isi":1,"year":"2025","publication":"Developmental Cell","language":[{"iso":"eng"}],"acknowledgement":"We thank A. Miller and N. Papalopulu for reagents and J. Briscoe for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European Research Council under Horizon Europe, grant 101044579; and the Austrian Science Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022).","file_date_updated":"2025-04-16T10:54:07Z","quality_controlled":"1","author":[{"orcid":"0000-0001-8703-1093","full_name":"Rus, Stefanie","first_name":"Stefanie","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","last_name":"Rus"},{"full_name":"Brückner, David","orcid":"0000-0001-7205-2975","first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","last_name":"Brückner"},{"full_name":"Minchington, Thomas","id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f","first_name":"Thomas","last_name":"Minchington"},{"full_name":"Greunz, Martina","first_name":"Martina","id":"48A59534-F248-11E8-B48F-1D18A9856A87","last_name":"Greunz"},{"last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"},{"last_name":"Kicheva","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","first_name":"Anna","full_name":"Kicheva, Anna","orcid":"0000-0003-4509-4998"}],"issue":"4","file":[{"success":1,"access_level":"open_access","creator":"dernst","content_type":"application/pdf","date_updated":"2025-04-16T10:54:07Z","file_size":6994499,"file_id":"19584","relation":"main_file","file_name":"2025_DevelopmentalCell_Lehr.pdf","checksum":"bb58db4a908a1f4aabe4004706154541","date_created":"2025-04-16T10:54:07Z"}],"abstract":[{"text":"Developing tissues interpret dynamic changes in morphogen activity to generate cell type diversity. To quantitatively study bone morphogenetic protein (BMP) signaling dynamics in the mouse neural tube, we developed an embryonic stem cell differentiation system tailored for growing tissues. Differentiating cells form striking self-organized patterns of dorsal neural tube cell types driven by sequential phases of BMP signaling that are observed both in vitro and in vivo. Data-driven biophysical modeling showed that these dynamics result from coupling fast negative feedback with slow positive regulation of signaling by the specification of an endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling in space, we identify a BMP signaling relay that operates in time. This mechanism allows for a rapid initial concentration-sensitive response that is robustly terminated, thereby regulating balanced sequential cell type generation. Our study provides an experimental and theoretical framework to understand how signaling dynamics are exploited in developing tissues.","lang":"eng"}],"page":"567-580","OA_type":"hybrid","article_processing_charge":"Yes (via OA deal)","date_created":"2025-01-09T11:25:47Z","date_published":"2025-02-24T00:00:00Z","OA_place":"publisher","status":"public","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"19763"}]},"external_id":{"pmid":["39603235"],"isi":["001434279000001"]},"article_type":"original","month":"02","date_updated":"2026-05-30T22:31:09Z","_id":"18807","corr_author":"1","title":"Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling","ddc":["570"],"type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","project":[{"grant_number":"101044579","name":"Mechanisms of tissue size regulation in spinal cord development","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa"},{"_id":"059DF620-7A3F-11EA-A408-12923DDC885E","grant_number":"F7802","name":"Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen control of growth and pattern in the spinal cord"},{"grant_number":"SC19-011","name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A"}],"has_accepted_license":"1","volume":60,"scopus_import":"1","day":"24","oa_version":"Published Version","doi":"10.1016/j.devcel.2024.10.024","publisher":"Elsevier","oa":1,"citation":{"short":"S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo, A. Kicheva, Developmental Cell 60 (2025) 567–580.","ama":"Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. Developmental Cell. 2025;60(4):567-580. doi:10.1016/j.devcel.2024.10.024","chicago":"Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2024.10.024.","ista":"Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva A. 2025. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.","apa":"Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E. B., & Kicheva, A. (2025). Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2024.10.024","ieee":"S. Rus et al., “Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling,” Developmental Cell, vol. 60, no. 4. Elsevier, pp. 567–580, 2025.","mla":"Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” Developmental Cell, vol. 60, no. 4, Elsevier, 2025, pp. 567–80, doi:10.1016/j.devcel.2024.10.024."},"intvolume":" 60","publication_status":"published","department":[{"_id":"AnKi"},{"_id":"EdHa"},{"_id":"NanoFab"}]},{"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"language":[{"iso":"eng"}],"year":"2024","isi":1,"publication":"Developmental Cell","acknowledgement":"This work was funded by DFG3468/6-1, DFG3468/6-3, and SFB924 to U.Z.H. We thank Angela Alkofer and Helene Prunkl for excellent technical assistance and Xenopus maintenance. Christian Luschnig is acknowledged for sharing unpublished results and valuable discussions.","file_date_updated":"2025-01-13T09:20:15Z","quality_controlled":"1","author":[{"last_name":"Janacek","full_name":"Janacek, DP","first_name":"DP"},{"last_name":"Kolb","first_name":"M","full_name":"Kolb, M"},{"last_name":"Schulz","first_name":"L","full_name":"Schulz, L"},{"last_name":"Mergner","full_name":"Mergner, J","first_name":"J"},{"full_name":"Kuster, B","first_name":"B","last_name":"Kuster"},{"id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","first_name":"Matous","full_name":"Glanc, Matous","orcid":"0000-0003-0619-7783","last_name":"Glanc"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"last_name":"Ten Tusscher","full_name":"Ten Tusscher, K","first_name":"K"},{"first_name":"C","full_name":"Schwechheimer, C","last_name":"Schwechheimer"},{"last_name":"Hammes","full_name":"Hammes, UZ","first_name":"UZ"}],"issue":"14","file":[{"success":1,"creator":"dernst","access_level":"open_access","content_type":"application/pdf","date_updated":"2025-01-13T09:20:15Z","file_size":3675955,"file_id":"18835","relation":"main_file","file_name":"2024_DevelopmentalCell_Janacek.pdf","checksum":"34423ee9fb4e30334f3572eddf1da2ae","date_created":"2025-01-13T09:20:15Z"}],"abstract":[{"lang":"eng","text":"The phytohormone auxin is polarly transported in plants by PIN-FORMED (PIN) transporters and controls virtually all growth and developmental processes. Canonical PINs possess a long, largely disordered cytosolic loop. Auxin transport by canonical PINs is activated by loop phosphorylation by certain kinases. The structure of the PIN transmembrane domains was recently determined, their transport properties remained poorly characterized, and the role of the loop in the transport process was unclear. Here, we determined the quantitative kinetic parameters of auxin transport mediated by Arabidopsis PINs to mathematically model auxin distribution in roots and to test these predictions in vivo. Using chimeras between transmembrane and loop domains of different PINs, we demonstrate a strong correlation between transport parameters and physiological output, indicating that the loop domain is not only required to activate PIN-mediated auxin transport, but it has an additional role in the transport process by a currently unknown mechanism."}],"OA_type":"hybrid","page":"S1534-5807(24)00569-0","article_processing_charge":"Yes (in subscription journal)","date_created":"2024-10-23T08:41:27Z","date_published":"2024-12-16T00:00:00Z","OA_place":"publisher","status":"public","external_id":{"pmid":["39413780"],"isi":["001390774300001"]},"license":"https://creativecommons.org/licenses/by-nc/4.0/","article_type":"original","month":"12","date_updated":"2025-09-08T14:33:17Z","_id":"18465","ddc":["570"],"title":"Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport","type":"journal_article","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"pmid":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","has_accepted_license":"1","volume":59,"scopus_import":"1","oa_version":"Published Version","day":"16","doi":"10.1016/j.devcel.2024.09.020","publisher":"Elsevier","oa":1,"citation":{"chicago":"Janacek, DP, M Kolb, L Schulz, J Mergner, B Kuster, Matous Glanc, Jiří Friml, K Ten Tusscher, C Schwechheimer, and UZ Hammes. “Transport Properties of Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” Developmental Cell. Elsevier, 2024. https://doi.org/10.1016/j.devcel.2024.09.020.","short":"D. Janacek, M. Kolb, L. Schulz, J. Mergner, B. Kuster, M. Glanc, J. Friml, K. Ten Tusscher, C. Schwechheimer, U. Hammes, Developmental Cell 59 (2024) S1534-5807(24)00569–0.","ama":"Janacek D, Kolb M, Schulz L, et al. Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. Developmental Cell. 2024;59(14):S1534-5807(24)00569-0. doi:10.1016/j.devcel.2024.09.020","ista":"Janacek D, Kolb M, Schulz L, Mergner J, Kuster B, Glanc M, Friml J, Ten Tusscher K, Schwechheimer C, Hammes U. 2024. Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. Developmental Cell. 59(14), S1534-5807(24)00569–0.","ieee":"D. Janacek et al., “Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport,” Developmental Cell, vol. 59, no. 14. Elsevier, pp. S1534-5807(24)00569–0, 2024.","apa":"Janacek, D., Kolb, M., Schulz, L., Mergner, J., Kuster, B., Glanc, M., … Hammes, U. (2024). Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2024.09.020","mla":"Janacek, DP, et al. “Transport Properties of Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” Developmental Cell, vol. 59, no. 14, Elsevier, 2024, pp. S1534-5807(24)00569-0, doi:10.1016/j.devcel.2024.09.020."},"intvolume":" 59","publication_status":"published","department":[{"_id":"JiFr"}]},{"month":"05","_id":"15301","date_updated":"2025-09-04T13:32:08Z","corr_author":"1","ddc":["570"],"title":"Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization","type":"journal_article","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"pmid":1,"ec_funded":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"P29988","name":"RNA-directed DNA methylation in plant development","call_identifier":"FWF","_id":"262EF96E-B435-11E9-9278-68D0E5697425"}],"has_accepted_license":"1","volume":59,"scopus_import":"1","doi":"10.1016/j.devcel.2024.03.009","day":"20","oa_version":"Published Version","publisher":"Elsevier","citation":{"ama":"Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 2024;59(10):1333-1344.e4. doi:10.1016/j.devcel.2024.03.009","short":"L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá, S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J. Friml, Developmental Cell 59 (2024) 1333–1344.e4.","chicago":"Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” Developmental Cell. Elsevier, 2024. https://doi.org/10.1016/j.devcel.2024.03.009.","mla":"Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” Developmental Cell, vol. 59, no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:10.1016/j.devcel.2024.03.009.","ista":"Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P, Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 59(10), 1333–1344.e4.","ieee":"L. Hörmayer et al., “Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization,” Developmental Cell, vol. 59, no. 10. Elsevier, p. 1333–1344.e4, 2024.","apa":"Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S., Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2024.03.009"},"oa":1,"intvolume":" 59","department":[{"_id":"JiFr"},{"_id":"EvBe"},{"_id":"CaHe"}],"publication_status":"published","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"year":"2024","isi":1,"language":[{"iso":"eng"}],"publication":"Developmental Cell","file_date_updated":"2024-08-20T11:22:16Z","acknowledgement":"We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder for sharing published material. We thank the Imaging & Optics and Life Science Facilities at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility at DBMV UNIL for providing invaluable assistance. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985, from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).","quality_controlled":"1","author":[{"first_name":"Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8295-2926","full_name":"Hörmayer, Lukas","last_name":"Hörmayer"},{"last_name":"Montesinos López","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C","orcid":"0000-0001-9179-6099","full_name":"Montesinos López, Juan C"},{"full_name":"Trozzi, N","first_name":"N","last_name":"Trozzi"},{"last_name":"Spona","id":"b52391fb-f636-11ee-939c-8a8c47552e8a","first_name":"Leonhard","full_name":"Spona, Leonhard"},{"last_name":"Yoshida","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","full_name":"Yoshida, Saiko"},{"first_name":"Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87","full_name":"Marhavá, Petra","last_name":"Marhavá"},{"last_name":"Caballero Mancebo","first_name":"Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","full_name":"Caballero Mancebo, Silvia","orcid":"0000-0002-5223-3346"},{"last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Dagdas","first_name":"Y","full_name":"Dagdas, Y"},{"last_name":"Majda","full_name":"Majda, M","first_name":"M"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"issue":"10","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"file":[{"file_id":"17452","file_size":5195262,"date_updated":"2024-08-20T11:22:16Z","content_type":"application/pdf","creator":"dernst","access_level":"open_access","success":1,"date_created":"2024-08-20T11:22:16Z","file_name":"2024_DevelopmentalCell_Hoermayer.pdf","checksum":"22b374fb50a40d380b7686c84258d271","relation":"main_file"}],"page":"1333-1344.e4","abstract":[{"lang":"eng","text":"Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture."}],"date_created":"2024-04-08T12:07:57Z","date_published":"2024-05-20T00:00:00Z","article_processing_charge":"Yes (via OA deal)","status":"public","related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/how-plants-heal-wounds/","description":"News on ISTA website"}]},"article_type":"original","external_id":{"isi":["001301584600001"],"pmid":["38579717"]}},{"quality_controlled":"1","author":[{"first_name":"Teresa","full_name":"Krammer, Teresa","last_name":"Krammer"},{"last_name":"Stuart","full_name":"Stuart, Hannah T.","first_name":"Hannah T."},{"last_name":"Gromberg","full_name":"Gromberg, Elena","first_name":"Elena"},{"full_name":"Ishihara, Keisuke","first_name":"Keisuke","last_name":"Ishihara"},{"first_name":"Dillon","full_name":"Cislo, Dillon","last_name":"Cislo"},{"last_name":"Melchionda","first_name":"Manuela","full_name":"Melchionda, Manuela"},{"full_name":"Becerril Perez, Fernando","first_name":"Fernando","last_name":"Becerril Perez"},{"last_name":"Wang","first_name":"Jingkui","full_name":"Wang, Jingkui"},{"full_name":"Costantini, Elena","first_name":"Elena","last_name":"Costantini"},{"first_name":"Stefanie","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8703-1093","full_name":"Rus, Stefanie","last_name":"Rus"},{"first_name":"Laura","full_name":"Arbanas, Laura","last_name":"Arbanas"},{"first_name":"Alexandra","full_name":"Hörmann, Alexandra","last_name":"Hörmann"},{"last_name":"Neumüller","first_name":"Ralph A.","full_name":"Neumüller, Ralph A."},{"last_name":"Elvassore","first_name":"Nicola","full_name":"Elvassore, Nicola"},{"first_name":"Eric","full_name":"Siggia, Eric","last_name":"Siggia"},{"first_name":"James","full_name":"Briscoe, James","last_name":"Briscoe"},{"last_name":"Kicheva","orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna","first_name":"Anna","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Elly M.","full_name":"Tanaka, Elly M.","last_name":"Tanaka"}],"issue":"15","file_date_updated":"2025-01-13T10:59:12Z","acknowledgement":"We thank P. Pasierbek, A.C. Moreno, T. Lendl, and K. Aumayr for microscopy support; G. Schmauss for FACS support; M. Novatchkova for assistance with Bioinformatic analyses; J. Ahel, A. Polikarpova, S. Horer, E. Cesare, and E. Norouzi for technical assistance; A. Meinhardt for supervision; DRESDEN-concept Genome Center, A. Vogt, A. Sommer, and the Vienna BioCenter NGS facility for RNA sequencing. We are grateful to M. Placzek and E. Martí for discussions about the floorplate; to S. Shvartsman for valuable input; to A. Aszodi, W. Masselink, and S. Raiders for advice on statistical analyses; to J. Cornwall Scoones, G. Martello, and Tanaka lab members for critical reading of the manuscript; E. Bassat and E. Chatzidaki for contributing schematics; and to K. Lust for support. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement ERC AdG 742046) to E.M.T. This research was funded in whole or in part by the Austrian Science Fund (FWF) (10.55776/F7803-B) (Stem Cell Modulation) to E.M.T. and A.K., Sir Henry Wellcome postdoctoral fellowship to H.T.S., ELBE fellowship to K.I., and National Science Foundation (US) Phy 2013131 to E.S. The A.K. lab is also supported by ISTA and the European Research Council under Horizon Europe grant 101044579, and S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. This work was supported in part by the Francis Crick Institute, which receives its core funding from Cancer Research UK (CC001051), the UK Medical Research Council (CC001051), and the Wellcome Trust (CC001051). For the purpose of open access, the authors have applied a CC BY public copyright license to any author accepted manuscript (AAM) version arising from this submission.","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"isi":1,"publication":"Developmental Cell","year":"2024","language":[{"iso":"eng"}],"related_material":{"record":[{"relation":"dissertation_contains","id":"19763","status":"public"}]},"external_id":{"pmid":["38776925"],"isi":["001289684800001"]},"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","article_type":"original","article_processing_charge":"Yes (in subscription journal)","date_published":"2024-08-01T00:00:00Z","date_created":"2024-06-16T22:01:07Z","status":"public","OA_place":"publisher","file":[{"relation":"main_file","checksum":"fefdea9c02862b4bb74de49b65ce638a","file_name":"2024_DevelopmentalCell_Krammer.pdf","date_created":"2025-01-13T10:59:12Z","access_level":"open_access","creator":"dernst","success":1,"content_type":"application/pdf","file_size":6249076,"date_updated":"2025-01-13T10:59:12Z","file_id":"18841"}],"abstract":[{"text":"During neural tube (NT) development, the notochord induces an organizer, the floorplate, which secretes Sonic Hedgehog (SHH) to pattern neural progenitors. Conversely, NT organoids (NTOs) from embryonic stem cells (ESCs) spontaneously form floorplates without the notochord, demonstrating that stem cells can self-organize without embryonic inducers. Here, we investigated floorplate self-organization in clonal mouse NTOs. Expression of the floorplate marker FOXA2 was initially spatially scattered before resolving into multiple clusters, which underwent competition and sorting, resulting in a stable “winning” floorplate. We identified that BMP signaling governed long-range cluster competition. FOXA2+ clusters expressed BMP4, suppressing FOXA2 in receiving cells while simultaneously expressing the BMP-inhibitor NOGGIN, promoting cluster persistence. Noggin mutation perturbed floorplate formation in NTOs and in the NT in vivo at mid/hindbrain regions, demonstrating how the floorplate can form autonomously without the notochord. Identifying the pathways governing organizer self-organization is critical for harnessing the developmental plasticity of stem cells in tissue engineering.","lang":"eng"}],"page":"1940-1953.e10","OA_type":"hybrid","type":"journal_article","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","pmid":1,"title":"Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition","ddc":["570"],"month":"08","date_updated":"2026-05-30T22:31:09Z","_id":"17148","intvolume":" 59","publication_status":"published","department":[{"_id":"AnKi"}],"publisher":"Elsevier","oa":1,"citation":{"ieee":"T. Krammer et al., “Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition,” Developmental Cell, vol. 59, no. 15. Elsevier, p. 1940–1953.e10, 2024.","ista":"Krammer T, Stuart HT, Gromberg E, Ishihara K, Cislo D, Melchionda M, Becerril Perez F, Wang J, Costantini E, Rus S, Arbanas L, Hörmann A, Neumüller RA, Elvassore N, Siggia E, Briscoe J, Kicheva A, Tanaka EM. 2024. Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition. Developmental Cell. 59(15), 1940–1953.e10.","apa":"Krammer, T., Stuart, H. T., Gromberg, E., Ishihara, K., Cislo, D., Melchionda, M., … Tanaka, E. M. (2024). Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2024.04.021","mla":"Krammer, Teresa, et al. “Mouse Neural Tube Organoids Self-Organize Floorplate through BMP-Mediated Cluster Competition.” Developmental Cell, vol. 59, no. 15, Elsevier, 2024, p. 1940–1953.e10, doi:10.1016/j.devcel.2024.04.021.","chicago":"Krammer, Teresa, Hannah T. Stuart, Elena Gromberg, Keisuke Ishihara, Dillon Cislo, Manuela Melchionda, Fernando Becerril Perez, et al. “Mouse Neural Tube Organoids Self-Organize Floorplate through BMP-Mediated Cluster Competition.” Developmental Cell. Elsevier, 2024. https://doi.org/10.1016/j.devcel.2024.04.021.","short":"T. Krammer, H.T. Stuart, E. Gromberg, K. Ishihara, D. Cislo, M. Melchionda, F. Becerril Perez, J. Wang, E. Costantini, S. Rus, L. Arbanas, A. Hörmann, R.A. Neumüller, N. Elvassore, E. Siggia, J. Briscoe, A. Kicheva, E.M. Tanaka, Developmental Cell 59 (2024) 1940–1953.e10.","ama":"Krammer T, Stuart HT, Gromberg E, et al. Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition. Developmental Cell. 2024;59(15):1940-1953.e10. doi:10.1016/j.devcel.2024.04.021"},"oa_version":"Published Version","day":"01","doi":"10.1016/j.devcel.2024.04.021","project":[{"name":"Mechanisms of tissue size regulation in spinal cord development","grant_number":"101044579","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa"},{"name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","grant_number":"SC19-011","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A"}],"scopus_import":"1","has_accepted_license":"1","volume":59},{"doi":"10.1016/j.devcel.2023.06.001","day":"07","oa_version":"Published Version","project":[{"_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d","name":"In vitro reconstitution of bacterial cell division","grant_number":"P34607"},{"_id":"bd6ae2ca-d553-11ed-ba76-a4aa239da5ee","name":"Synthetic and structural biology of Rab GTPase networks","grant_number":"101045340"}],"scopus_import":"1","has_accepted_license":"1","volume":58,"intvolume":" 58","department":[{"_id":"MaLo"}],"publication_status":"published","publisher":"Elsevier","citation":{"chicago":"Leonard, Thomas A., Martin Loose, and Sascha Martens. “The Membrane Surface as a Platform That Organizes Cellular and Biochemical Processes.” Developmental Cell. Elsevier, 2023. https://doi.org/10.1016/j.devcel.2023.06.001.","ama":"Leonard TA, Loose M, Martens S. The membrane surface as a platform that organizes cellular and biochemical processes. Developmental Cell. 2023;58(15):1315-1332. doi:10.1016/j.devcel.2023.06.001","short":"T.A. Leonard, M. Loose, S. Martens, Developmental Cell 58 (2023) 1315–1332.","ista":"Leonard TA, Loose M, Martens S. 2023. The membrane surface as a platform that organizes cellular and biochemical processes. Developmental Cell. 58(15), 1315–1332.","apa":"Leonard, T. A., Loose, M., & Martens, S. (2023). The membrane surface as a platform that organizes cellular and biochemical processes. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2023.06.001","ieee":"T. A. Leonard, M. Loose, and S. Martens, “The membrane surface as a platform that organizes cellular and biochemical processes,” Developmental Cell, vol. 58, no. 15. Elsevier, pp. 1315–1332, 2023.","mla":"Leonard, Thomas A., et al. “The Membrane Surface as a Platform That Organizes Cellular and Biochemical Processes.” Developmental Cell, vol. 58, no. 15, Elsevier, 2023, pp. 1315–32, doi:10.1016/j.devcel.2023.06.001."},"oa":1,"corr_author":"1","ddc":["570"],"title":"The membrane surface as a platform that organizes cellular and biochemical processes","month":"08","_id":"14039","date_updated":"2024-10-22T11:40:18Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"file":[{"success":1,"creator":"dernst","access_level":"open_access","content_type":"application/pdf","date_updated":"2023-08-14T07:57:55Z","file_size":3184217,"file_id":"14049","relation":"main_file","checksum":"d8c5dc97cd40c26da2ec98ae723ab368","file_name":"2023_DevelopmentalCell_Leonard.pdf","date_created":"2023-08-14T07:57:55Z"}],"page":"1315-1332","abstract":[{"text":"Membranes are essential for life. They act as semi-permeable boundaries that define cells and organelles. In addition, their surfaces actively participate in biochemical reaction networks, where they confine proteins, align reaction partners, and directly control enzymatic activities. Membrane-localized reactions shape cellular membranes, define the identity of organelles, compartmentalize biochemical processes, and can even be the source of signaling gradients that originate at the plasma membrane and reach into the cytoplasm and nucleus. The membrane surface is, therefore, an essential platform upon which myriad cellular processes are scaffolded. In this review, we summarize our current understanding of the biophysics and biochemistry of membrane-localized reactions with particular focus on insights derived from reconstituted and cellular systems. We discuss how the interplay of cellular factors results in their self-organization, condensation, assembly, and activity, and the emergent properties derived from them.","lang":"eng"}],"article_type":"original","external_id":{"isi":["001059110400001"],"pmid":["37419118"]},"date_published":"2023-08-07T00:00:00Z","date_created":"2023-08-13T22:01:12Z","article_processing_charge":"Yes (via OA deal)","status":"public","file_date_updated":"2023-08-14T07:57:55Z","acknowledgement":"We acknowledge funding from the Austrian Science Fund (FWF F79, P32814-B, and P35061-B to S.M.; P34607-B to M.L.; and P30584-B and P33066-B to T.A.L.) and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 101045340 to M.L.). We are grateful for comments on the manuscript by Justyna Sawa-Makarska, Verena Baumann, Marko Kojic, Philipp Radler, Ronja Reinhardt, and Sumire Antonioli.","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"year":"2023","isi":1,"language":[{"iso":"eng"}],"publication":"Developmental Cell","quality_controlled":"1","issue":"15","author":[{"full_name":"Leonard, Thomas A.","first_name":"Thomas A.","last_name":"Leonard"},{"orcid":"0000-0001-7309-9724","full_name":"Loose, Martin","first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose"},{"last_name":"Martens","first_name":"Sascha","full_name":"Martens, Sascha"}]},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","ec_funded":1,"_id":"12830","date_updated":"2025-04-23T08:51:34Z","month":"04","title":"A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish","ddc":["570"],"corr_author":"1","citation":{"short":"K. Huljev, S. Shamipour, D.C. Nunes Pinheiro, F. Preusser, I. Steccari, C.M. Sommer, S. Naik, C.-P.J. Heisenberg, Developmental Cell 58 (2023) 582–596.e7.","ama":"Huljev K, Shamipour S, Nunes Pinheiro DC, et al. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. 2023;58(7):582-596.e7. doi:10.1016/j.devcel.2023.02.016","chicago":"Huljev, Karla, Shayan Shamipour, Diana C Nunes Pinheiro, Friedrich Preusser, Irene Steccari, Christoph M Sommer, Suyash Naik, and Carl-Philipp J Heisenberg. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” Developmental Cell. Elsevier, 2023. https://doi.org/10.1016/j.devcel.2023.02.016.","mla":"Huljev, Karla, et al. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” Developmental Cell, vol. 58, no. 7, Elsevier, 2023, p. 582–596.e7, doi:10.1016/j.devcel.2023.02.016.","apa":"Huljev, K., Shamipour, S., Nunes Pinheiro, D. C., Preusser, F., Steccari, I., Sommer, C. M., … Heisenberg, C.-P. J. (2023). A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2023.02.016","ieee":"K. Huljev et al., “A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish,” Developmental Cell, vol. 58, no. 7. Elsevier, p. 582–596.e7, 2023.","ista":"Huljev K, Shamipour S, Nunes Pinheiro DC, Preusser F, Steccari I, Sommer CM, Naik S, Heisenberg C-PJ. 2023. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. 58(7), 582–596.e7."},"oa":1,"publisher":"Elsevier","department":[{"_id":"CaHe"},{"_id":"Bio"}],"publication_status":"published","intvolume":" 58","scopus_import":"1","volume":58,"has_accepted_license":"1","project":[{"grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"_id":"26520D1E-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 850-2017","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation"},{"_id":"266BC5CE-B435-11E9-9278-68D0E5697425","grant_number":"LT000429","name":"Coordination of mesendoderm fate specification and internalization during zebrafish gastrulation"}],"doi":"10.1016/j.devcel.2023.02.016","oa_version":"Published Version","day":"10","author":[{"id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","first_name":"Karla","full_name":"Huljev, Karla","last_name":"Huljev"},{"last_name":"Shamipour","full_name":"Shamipour, Shayan","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Nunes Pinheiro","orcid":"0000-0003-4333-7503","full_name":"Nunes Pinheiro, Diana C","id":"2E839F16-F248-11E8-B48F-1D18A9856A87","first_name":"Diana C"},{"last_name":"Preusser","first_name":"Friedrich","full_name":"Preusser, Friedrich"},{"last_name":"Steccari","id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene","full_name":"Steccari, Irene"},{"last_name":"Sommer","first_name":"Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M","orcid":"0000-0003-1216-9105"},{"orcid":"0000-0001-8421-5508","full_name":"Naik, Suyash","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","first_name":"Suyash","last_name":"Naik"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg"}],"issue":"7","quality_controlled":"1","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"publication":"Developmental Cell","isi":1,"year":"2023","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"acknowledgement":"We thank Andrea Pauli (IMP) and Edouard Hannezo (ISTA) for fruitful discussions and support with the SPIM experiments; the Heisenberg group, and especially Feyza Nur Arslan and Alexandra Schauer, for discussions and feedback; Michaela Jović (ISTA) for help with the quantitative real-time PCR protocol; the bioimaging and zebrafish facilities of ISTA for continuous support; Stephan Preibisch (Janelia Research Campus) for support with the SPIM data analysis; and Nobuhiro Nakamura (Tokyo Institute of Technology) for sharing α1-Na+/K+-ATPase antibody. This work was supported by funding from the European Union (European Research Council Advanced grant 742573 to C.-P.H.), postdoctoral fellowships from EMBO (LTF-850-2017) and HFSP (LT000429/2018-L2) to D.P., and a PhD fellowship from the Studienstiftung des deutschen Volkes to F.P.","file_date_updated":"2023-04-17T07:41:25Z","status":"public","date_published":"2023-04-10T00:00:00Z","date_created":"2023-04-16T22:01:07Z","article_processing_charge":"Yes (via OA deal)","article_type":"original","external_id":{"pmid":["36931269"],"isi":["000982111800001"]},"page":"582-596.e7","abstract":[{"lang":"eng","text":"Interstitial fluid (IF) accumulation between embryonic cells is thought to be important for embryo patterning and morphogenesis. Here, we identify a positive mechanical feedback loop between cell migration and IF relocalization and find that it promotes embryonic axis formation during zebrafish gastrulation. We show that anterior axial mesendoderm (prechordal plate [ppl]) cells, moving in between the yolk cell and deep cell tissue to extend the embryonic axis, compress the overlying deep cell layer, thereby causing IF to flow from the deep cell layer to the boundary between the yolk cell and the deep cell layer, directly ahead of the advancing ppl. This IF relocalization, in turn, facilitates ppl cell protrusion formation and migration by opening up the space into which the ppl moves and, thereby, the ability of the ppl to trigger IF relocalization by pushing against the overlying deep cell layer. Thus, embryonic axis formation relies on a hydraulic feedback loop between cell migration and IF relocalization."}],"file":[{"content_type":"application/pdf","access_level":"open_access","creator":"dernst","success":1,"file_id":"12842","file_size":7925886,"date_updated":"2023-04-17T07:41:25Z","relation":"main_file","date_created":"2023-04-17T07:41:25Z","checksum":"c80ca2ebc241232aacdb5aa4b4c80957","file_name":"2023_DevelopmentalCell_Huljev.pdf"}]},{"title":"Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1","_id":"14781","date_updated":"2024-01-16T08:56:36Z","month":"09","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"type":"journal_article","doi":"10.1016/j.devcel.2023.06.009","oa_version":"Preprint","day":"11","main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2023.07.09.548244"}],"volume":58,"department":[{"_id":"Bio"}],"publication_status":"published","intvolume":" 58","keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"citation":{"chicago":"Westerich, Kim Joana, Katsiaryna Tarbashevich, Jan Schick, Antra Gupta, Mingzhao Zhu, Kenneth Hull, Daniel Romo, et al. “Spatial Organization and Function of RNA Molecules within Phase-Separated Condensates in Zebrafish Are Controlled by Dnd1.” Developmental Cell. Elsevier, 2023. https://doi.org/10.1016/j.devcel.2023.06.009.","short":"K.J. Westerich, K. Tarbashevich, J. Schick, A. Gupta, M. Zhu, K. Hull, D. Romo, D. Zeuschner, M. Goudarzi, T. Gross-Thebing, E. Raz, Developmental Cell 58 (2023) 1578–1592.e5.","ama":"Westerich KJ, Tarbashevich K, Schick J, et al. Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1. Developmental Cell. 2023;58(17):1578-1592.e5. doi:10.1016/j.devcel.2023.06.009","mla":"Westerich, Kim Joana, et al. “Spatial Organization and Function of RNA Molecules within Phase-Separated Condensates in Zebrafish Are Controlled by Dnd1.” Developmental Cell, vol. 58, no. 17, Elsevier, 2023, p. 1578–1592.e5, doi:10.1016/j.devcel.2023.06.009.","ista":"Westerich KJ, Tarbashevich K, Schick J, Gupta A, Zhu M, Hull K, Romo D, Zeuschner D, Goudarzi M, Gross-Thebing T, Raz E. 2023. Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1. Developmental Cell. 58(17), 1578–1592.e5.","ieee":"K. J. Westerich et al., “Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1,” Developmental Cell, vol. 58, no. 17. Elsevier, p. 1578–1592.e5, 2023.","apa":"Westerich, K. J., Tarbashevich, K., Schick, J., Gupta, A., Zhu, M., Hull, K., … Raz, E. (2023). Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2023.06.009"},"oa":1,"publisher":"Elsevier","acknowledgement":"We thank Celeste Brennecka for editing and Michal Reichman-Fried for critical comments on the manuscript. We thank Ursula Jordan, Esther Messerschmidt, and Ines Sandbote for technical assistance. This work was supported by funding from the University of Münster (K.J.W., K.T., E.R., A.G., T.G.-T., J.S., and M.G.), the Max Planck Institute for Molecular Biomedicine (D.Z.), the German Research Foundation grant CRU 326 (P2) RA863/12-2 (E.R.), Baylor University (K.H. and D.R.), and the National Institutes of Health grant R35 GM 134910 (D.R.). We thank the referees for insightful comments that helped improve the manuscript.","language":[{"iso":"eng"}],"year":"2023","publication":"Developmental Cell","publication_identifier":{"issn":["1534-5807"]},"issue":"17","author":[{"last_name":"Westerich","full_name":"Westerich, Kim Joana","first_name":"Kim Joana"},{"last_name":"Tarbashevich","first_name":"Katsiaryna","full_name":"Tarbashevich, Katsiaryna"},{"last_name":"Schick","full_name":"Schick, Jan","first_name":"Jan"},{"last_name":"Gupta","first_name":"Antra","full_name":"Gupta, Antra"},{"last_name":"Zhu","first_name":"Mingzhao","full_name":"Zhu, Mingzhao"},{"last_name":"Hull","full_name":"Hull, Kenneth","first_name":"Kenneth"},{"last_name":"Romo","full_name":"Romo, Daniel","first_name":"Daniel"},{"last_name":"Zeuschner","first_name":"Dagmar","full_name":"Zeuschner, Dagmar"},{"last_name":"Goudarzi","id":"3384113A-F248-11E8-B48F-1D18A9856A87","first_name":"Mohammad","full_name":"Goudarzi, Mohammad"},{"first_name":"Theresa","full_name":"Gross-Thebing, Theresa","last_name":"Gross-Thebing"},{"last_name":"Raz","first_name":"Erez","full_name":"Raz, Erez"}],"quality_controlled":"1","page":"1578-1592.e5","abstract":[{"text":"Germ granules, condensates of phase-separated RNA and protein, are organelles that are essential for germline development in different organisms. The patterning of the granules and their relevance for germ cell fate are not fully understood. Combining three-dimensional in vivo structural and functional analyses, we study the dynamic spatial organization of molecules within zebrafish germ granules. We find that the localization of RNA molecules to the periphery of the granules, where ribosomes are localized, depends on translational activity at this location. In addition, we find that the vertebrate-specific Dead end (Dnd1) protein is essential for nanos3 RNA localization at the condensates’ periphery. Accordingly, in the absence of Dnd1, or when translation is inhibited, nanos3 RNA translocates into the granule interior, away from the ribosomes, a process that is correlated with the loss of germ cell fate. These findings highlight the relevance of sub-granule compartmentalization for post-transcriptional control and its importance for preserving germ cell totipotency.","lang":"eng"}],"article_type":"original","external_id":{"pmid":["37463577"]},"status":"public","date_created":"2024-01-10T09:41:21Z","date_published":"2023-09-11T00:00:00Z","article_processing_charge":"No"},{"author":[{"full_name":"Xiao, Huixin","first_name":"Huixin","last_name":"Xiao"},{"full_name":"Hu, Yumei","first_name":"Yumei","last_name":"Hu"},{"full_name":"Wang, Yaping","first_name":"Yaping","last_name":"Wang"},{"full_name":"Cheng, Jinkui","first_name":"Jinkui","last_name":"Cheng"},{"last_name":"Wang","first_name":"Jinyi","full_name":"Wang, Jinyi"},{"full_name":"Chen, Guojingwei","first_name":"Guojingwei","last_name":"Chen"},{"last_name":"Li","first_name":"Qian","full_name":"Li, Qian"},{"last_name":"Wang","first_name":"Shuwei","full_name":"Wang, Shuwei"},{"last_name":"Wang","first_name":"Yalu","full_name":"Wang, Yalu"},{"first_name":"Shao-Shuai","full_name":"Wang, Shao-Shuai","last_name":"Wang"},{"full_name":"Wang, Yi","first_name":"Yi","last_name":"Wang"},{"last_name":"Xuan","first_name":"Wei","full_name":"Xuan, Wei"},{"last_name":"Li","first_name":"Zhen","full_name":"Li, Zhen"},{"last_name":"Guo","full_name":"Guo, Yan","first_name":"Yan"},{"last_name":"Gong","full_name":"Gong, Zhizhong","first_name":"Zhizhong"},{"full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"},{"last_name":"Zhang","full_name":"Zhang, Jing","first_name":"Jing"}],"issue":"23","quality_controlled":"1","acknowledgement":"The authors are grateful to Jörg Kudla, Ying Miao, Yu Zheng, Gang Li, and Jun Zheng for providing published materials and to Wenkun Zhou and Caifu Jiang for helpful discussions. This work was supported by grants from the National Key Research and Development Program of China (2021YFF1000500), the National Natural Science Foundation of China (32170265 and 32022007), the Beijing Municipal Natural Science Foundation (5192011), and the Chinese Universities Scientific Fund (2022TC153).","publication":"Developmental Cell","language":[{"iso":"eng"}],"isi":1,"year":"2022","publication_identifier":{"issn":["1534-5807"]},"article_type":"original","external_id":{"pmid":["36473460"],"isi":["000919603800005"]},"OA_place":"publisher","status":"public","date_created":"2023-01-12T11:57:00Z","date_published":"2022-12-05T00:00:00Z","article_processing_charge":"No","page":"2638-2651.e6","OA_type":"free access","abstract":[{"lang":"eng","text":"Plant root architecture flexibly adapts to changing nitrate (NO3−) availability in the soil; however, the underlying molecular mechanism of this adaptive development remains under-studied. To explore the regulation of NO3−-mediated root growth, we screened for low-nitrate-resistant mutant (lonr) and identified mutants that were defective in the NAC transcription factor NAC075 (lonr1) as being less sensitive to low NO3− in terms of primary root growth. We show that NAC075 is a mobile transcription factor relocating from the root stele tissues to the endodermis based on NO3− availability. Under low-NO3− availability, the kinase CBL-interacting protein kinase 1 (CIPK1) is activated, and it phosphorylates NAC075, restricting its movement from the stele, which leads to the transcriptional regulation of downstream target WRKY53, consequently leading to adapted root architecture. Our work thus identifies an adaptive mechanism involving translocation of transcription factor based on nutrient availability and leading to cell-specific reprogramming of plant root growth."}],"pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","title":"Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth","_id":"12120","date_updated":"2025-06-25T07:29:52Z","month":"12","department":[{"_id":"JiFr"}],"publication_status":"published","intvolume":" 57","keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"citation":{"apa":"Xiao, H., Hu, Y., Wang, Y., Cheng, J., Wang, J., Chen, G., … Zhang, J. (2022). Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2022.11.006","ieee":"H. Xiao et al., “Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth,” Developmental Cell, vol. 57, no. 23. Elsevier, p. 2638–2651.e6, 2022.","ista":"Xiao H, Hu Y, Wang Y, Cheng J, Wang J, Chen G, Li Q, Wang S, Wang Y, Wang S-S, Wang Y, Xuan W, Li Z, Guo Y, Gong Z, Friml J, Zhang J. 2022. Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth. Developmental Cell. 57(23), 2638–2651.e6.","mla":"Xiao, Huixin, et al. “Nitrate Availability Controls Translocation of the Transcription Factor NAC075 for Cell-Type-Specific Reprogramming of Root Growth.” Developmental Cell, vol. 57, no. 23, Elsevier, 2022, p. 2638–2651.e6, doi:10.1016/j.devcel.2022.11.006.","ama":"Xiao H, Hu Y, Wang Y, et al. Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth. Developmental Cell. 2022;57(23):2638-2651.e6. doi:10.1016/j.devcel.2022.11.006","short":"H. Xiao, Y. Hu, Y. Wang, J. Cheng, J. Wang, G. Chen, Q. Li, S. Wang, Y. Wang, S.-S. Wang, Y. Wang, W. Xuan, Z. Li, Y. Guo, Z. Gong, J. Friml, J. Zhang, Developmental Cell 57 (2022) 2638–2651.e6.","chicago":"Xiao, Huixin, Yumei Hu, Yaping Wang, Jinkui Cheng, Jinyi Wang, Guojingwei Chen, Qian Li, et al. “Nitrate Availability Controls Translocation of the Transcription Factor NAC075 for Cell-Type-Specific Reprogramming of Root Growth.” Developmental Cell. Elsevier, 2022. https://doi.org/10.1016/j.devcel.2022.11.006."},"oa":1,"publisher":"Elsevier","doi":"10.1016/j.devcel.2022.11.006","oa_version":"Published Version","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2022.11.006","open_access":"1"}],"day":"05","scopus_import":"1","volume":57},{"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"month":"10","_id":"12238","date_updated":"2025-06-25T07:35:27Z","corr_author":"1","title":"A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration","publisher":"Elsevier","citation":{"mla":"Hino, Naoya, et al. “A Feedback Loop between Lamellipodial Extension and HGF-ERK Signaling Specifies Leader Cells during Collective Cell Migration.” Developmental Cell, vol. 57, no. 19, Elsevier, 2022, p. 2290–2304.e7, doi:10.1016/j.devcel.2022.09.003.","ista":"Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, Tsukiji S, Aoki K, Terai K, Hirashima T, Trepat X, Matsuda M. 2022. A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. Developmental Cell. 57(19), 2290–2304.e7.","ieee":"N. Hino et al., “A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration,” Developmental Cell, vol. 57, no. 19. Elsevier, p. 2290–2304.e7, 2022.","apa":"Hino, N., Matsuda, K., Jikko, Y., Maryu, G., Sakai, K., Imamura, R., … Matsuda, M. (2022). A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2022.09.003","short":"N. Hino, K. Matsuda, Y. Jikko, G. Maryu, K. Sakai, R. Imamura, S. Tsukiji, K. Aoki, K. Terai, T. Hirashima, X. Trepat, M. Matsuda, Developmental Cell 57 (2022) 2290–2304.e7.","ama":"Hino N, Matsuda K, Jikko Y, et al. A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. Developmental Cell. 2022;57(19):2290-2304.e7. doi:10.1016/j.devcel.2022.09.003","chicago":"Hino, Naoya, Kimiya Matsuda, Yuya Jikko, Gembu Maryu, Katsuya Sakai, Ryu Imamura, Shinya Tsukiji, et al. “A Feedback Loop between Lamellipodial Extension and HGF-ERK Signaling Specifies Leader Cells during Collective Cell Migration.” Developmental Cell. Elsevier, 2022. https://doi.org/10.1016/j.devcel.2022.09.003."},"oa":1,"intvolume":" 57","keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"department":[{"_id":"CaHe"}],"publication_status":"published","scopus_import":"1","volume":57,"doi":"10.1016/j.devcel.2022.09.003","oa_version":"Published Version","day":"01","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2022.09.003"}],"quality_controlled":"1","author":[{"last_name":"Hino","full_name":"Hino, Naoya","first_name":"Naoya","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307"},{"first_name":"Kimiya","full_name":"Matsuda, Kimiya","last_name":"Matsuda"},{"last_name":"Jikko","first_name":"Yuya","full_name":"Jikko, Yuya"},{"full_name":"Maryu, Gembu","first_name":"Gembu","last_name":"Maryu"},{"full_name":"Sakai, Katsuya","first_name":"Katsuya","last_name":"Sakai"},{"last_name":"Imamura","first_name":"Ryu","full_name":"Imamura, Ryu"},{"last_name":"Tsukiji","full_name":"Tsukiji, Shinya","first_name":"Shinya"},{"full_name":"Aoki, Kazuhiro","first_name":"Kazuhiro","last_name":"Aoki"},{"last_name":"Terai","first_name":"Kenta","full_name":"Terai, Kenta"},{"first_name":"Tsuyoshi","full_name":"Hirashima, Tsuyoshi","last_name":"Hirashima"},{"full_name":"Trepat, Xavier","first_name":"Xavier","last_name":"Trepat"},{"full_name":"Matsuda, Michiyuki","first_name":"Michiyuki","last_name":"Matsuda"}],"issue":"19","publication_identifier":{"issn":["1534-5807"]},"year":"2022","language":[{"iso":"eng"}],"isi":1,"publication":"Developmental Cell","acknowledgement":"We thank the members of the Matsuda Laboratory for their helpful discussion and encouragement, and we thank K. Hirano and K. Takakura for their technical assistance. This work was supported by the Kyoto University Live Imaging Center. Financial support was provided in the form of JSPS KAKENHI grants (nos. 17J02107 and 20K22653 to N.H., and 20H05898 and 19H00993 to M.M.), a JST CREST grant (no. JPMJCR1654 to M.M.), a Moonshot R&D grant (no. JPMJPS2022-11 to M.M.), Generalitat de Catalunya and the CERCA Programme (no. SGR-2017-01602 to X.T.), MICCINN/FEDER (no. PGC2018-099645-B-I00 to X.T.), and European Research Council (no. Adv-883739 to X.T.). IBEC is a recipient of a Severo Ochoa Award of Excellence from the MINECO. This work was partly supported by an Extramural Collaborative Research Grant of Cancer Research Institute, Kanazawa University.","date_published":"2022-10-01T00:00:00Z","date_created":"2023-01-16T09:51:39Z","article_processing_charge":"No","OA_place":"publisher","status":"public","article_type":"original","external_id":{"isi":["000898428700006"],"pmid":["36174555"]},"OA_type":"free access","page":"2290-2304.e7","abstract":[{"lang":"eng","text":"Upon the initiation of collective cell migration, the cells at the free edge are specified as leader cells; however, the mechanism underlying the leader cell specification remains elusive. Here, we show that lamellipodial extension after the release from mechanical confinement causes sustained extracellular signal-regulated kinase (ERK) activation and underlies the leader cell specification. Live-imaging of Madin-Darby canine kidney (MDCK) cells and mouse epidermis through the use of Förster resonance energy transfer (FRET)-based biosensors showed that leader cells exhibit sustained ERK activation in a hepatocyte growth factor (HGF)-dependent manner. Meanwhile, follower cells exhibit oscillatory ERK activation waves in an epidermal growth factor (EGF) signaling-dependent manner. Lamellipodial extension at the free edge increases the cellular sensitivity to HGF. The HGF-dependent ERK activation, in turn, promotes lamellipodial extension, thereby forming a positive feedback loop between cell extension and ERK activation and specifying the cells at the free edge as the leader cells. Our findings show that the integration of physical and biochemical cues underlies the leader cell specification during collective cell migration."}]},{"title":"A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis","_id":"10714","date_updated":"2025-06-12T06:19:50Z","month":"04","ec_funded":1,"tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"type":"journal_article","doi":"10.1016/j.devcel.2022.03.005","main_file_link":[{"url":"https://doi.org/10.1101/2021.04.04.438367","open_access":"1"}],"day":"11","oa_version":"Preprint","volume":57,"scopus_import":"1","project":[{"_id":"2536F660-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"334077","name":"Investigating the role of transporters in invasive migration through junctions"},{"call_identifier":"FWF","_id":"253B6E48-B435-11E9-9278-68D0E5697425","name":"The role of Drosophila TNF alpha in immune cell invasion","grant_number":"P29638"}],"department":[{"_id":"DaSi"}],"publication_status":"published","intvolume":" 57","citation":{"short":"E.T. Martin, P. Blatt, E. Ngyuen, R. Lahr, S. Selvam, H.A.M. Yoon, T. Pocchiari, S. Emtenani, D.E. Siekhaus, A. Berman, G. Fuchs, P. Rangan, Developmental Cell 57 (2022) 883–900.e10.","ama":"Martin ET, Blatt P, Ngyuen E, et al. A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis. Developmental Cell. 2022;57(7):883-900.e10. doi:10.1016/j.devcel.2022.03.005","chicago":"Martin, Elliot T., Patrick Blatt, Elaine Ngyuen, Roni Lahr, Sangeetha Selvam, Hyun Ah M. Yoon, Tyler Pocchiari, et al. “A Translation Control Module Coordinates Germline Stem Cell Differentiation with Ribosome Biogenesis during Drosophila Oogenesis.” Developmental Cell. Elsevier, 2022. https://doi.org/10.1016/j.devcel.2022.03.005.","mla":"Martin, Elliot T., et al. “A Translation Control Module Coordinates Germline Stem Cell Differentiation with Ribosome Biogenesis during Drosophila Oogenesis.” Developmental Cell, vol. 57, no. 7, Elsevier, 2022, p. 883–900.e10, doi:10.1016/j.devcel.2022.03.005.","apa":"Martin, E. T., Blatt, P., Ngyuen, E., Lahr, R., Selvam, S., Yoon, H. A. M., … Rangan, P. (2022). A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2022.03.005","ista":"Martin ET, Blatt P, Ngyuen E, Lahr R, Selvam S, Yoon HAM, Pocchiari T, Emtenani S, Siekhaus DE, Berman A, Fuchs G, Rangan P. 2022. A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis. Developmental Cell. 57(7), 883–900.e10.","ieee":"E. T. Martin et al., “A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis,” Developmental Cell, vol. 57, no. 7. Elsevier, p. 883–900.e10, 2022."},"oa":1,"publisher":"Elsevier","acknowledgement":"We are grateful to all members of the Rangan and Fuchs labs for their discussion and comments on the manuscript. We also thanks Dr. Sammons, Dr. Marlow, Life Science Editors, for their thoughts and comments the manuscript Additionally, we thank the Bloomington Stock Center, the Vienna Drosophila Resource Center, the BDGP Gene Disruption Project, and Flybase for fly stocks, reagents, and other resources. P.R. is funded by the NIH/NIGMS (R01GM111779-06 and RO1GM135628-01), G.F. is funded by NSF MCB-2047629 and NIH RO3 AI144839, D.E.S. was funded by Marie Curie CIG 334077/IRTIM and the Austrian Science Fund (FWF) grant ASI_FWF01_P29638S, and A.B is funded by NIH R01GM116889 and American Cancer Society RSG-17-197-01-RMC.","publication":"Developmental Cell","language":[{"iso":"eng"}],"isi":1,"year":"2022","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"issue":"7","author":[{"last_name":"Martin","full_name":"Martin, Elliot T.","first_name":"Elliot T."},{"full_name":"Blatt, Patrick","first_name":"Patrick","last_name":"Blatt"},{"full_name":"Ngyuen, Elaine","first_name":"Elaine","last_name":"Ngyuen"},{"last_name":"Lahr","first_name":"Roni","full_name":"Lahr, Roni"},{"last_name":"Selvam","first_name":"Sangeetha","full_name":"Selvam, Sangeetha"},{"last_name":"Yoon","first_name":"Hyun Ah M.","full_name":"Yoon, Hyun Ah M."},{"full_name":"Pocchiari, Tyler","first_name":"Tyler","last_name":"Pocchiari"},{"last_name":"Emtenani","full_name":"Emtenani, Shamsi","orcid":"0000-0001-6981-6938","first_name":"Shamsi","id":"49D32318-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-8323-8353","full_name":"Siekhaus, Daria E","first_name":"Daria E","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","last_name":"Siekhaus"},{"last_name":"Berman","full_name":"Berman, Andrea","first_name":"Andrea"},{"full_name":"Fuchs, Gabriele","first_name":"Gabriele","last_name":"Fuchs"},{"full_name":"Rangan, Prashanth","first_name":"Prashanth","last_name":"Rangan"}],"quality_controlled":"1","page":"883-900.e10","abstract":[{"text":"Ribosomal defects perturb stem cell differentiation, causing diseases called ribosomopathies. How ribosome levels control stem cell differentiation is not fully known. Here, we discovered three RNA helicases are required for ribosome biogenesis and for Drosophila oogenesis. Loss of these helicases, which we named Aramis, Athos and Porthos, lead to aberrant stabilization of p53, cell cycle arrest and stalled GSC differentiation. Unexpectedly, Aramis is required for efficient translation of a cohort of mRNAs containing a 5’-Terminal-Oligo-Pyrimidine (TOP)-motif, including mRNAs that encode ribosomal proteins and a conserved p53 inhibitor, Novel Nucleolar protein 1 (Non1). The TOP-motif co-regulates the translation of growth-related mRNAs in mammals. As in mammals, the La-related protein co-regulates the translation of TOP-motif containing RNAs during Drosophila oogenesis. Thus, a previously unappreciated TOP-motif in Drosophila responds to reduced ribosome biogenesis to co-regulate the translation of ribosomal proteins and a p53 repressor, thus coupling ribosome biogenesis to GSC differentiation.","lang":"eng"}],"article_type":"original","external_id":{"pmid":["35413237"],"isi":["000789021800005"]},"status":"public","date_created":"2022-02-01T13:15:05Z","date_published":"2022-04-11T00:00:00Z","article_processing_charge":"No"},{"isi":1,"publication":"Developmental Cell","year":"2022","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"acknowledgement":"We thank N. Darwish-Miranda, F. Leite, F.P. Assen, and A. Eichner for advice and help with experiments. We thank J. Renkawitz, E. Kiermaier, A. Juanes Garcia, and M. Avellaneda for critical reading of the manuscript. We thank M. Driscoll for advice on fluorescent labeling of collagen gels. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by Molecular Biology Services/Lab Support Facility (LSF)/Bioimaging Facility/Electron Microscopy Facility. This work was funded by grants from the European Research Council ( CoG 724373 ) and the Austrian Science Foundation (FWF) to M.S. F.G. received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 747687.","issue":"1","author":[{"last_name":"Gaertner","full_name":"Gaertner, Florian","first_name":"Florian"},{"full_name":"Dos Reis Rodrigues, Patricia","orcid":"0000-0003-1681-508X","first_name":"Patricia","id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","last_name":"Dos Reis Rodrigues"},{"full_name":"De Vries, Ingrid","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","last_name":"De Vries"},{"last_name":"Hons","full_name":"Hons, Miroslav","orcid":"0000-0002-6625-3348","first_name":"Miroslav","id":"4167FE56-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Aguilera","first_name":"Juan","full_name":"Aguilera, Juan"},{"last_name":"Riedl","full_name":"Riedl, Michael","orcid":"0000-0003-4844-6311","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","first_name":"Michael"},{"last_name":"Leithner","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander F","orcid":"0000-0002-1073-744X","full_name":"Leithner, Alexander F"},{"id":"4323B49C-F248-11E8-B48F-1D18A9856A87","first_name":"Saren","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X","last_name":"Tasciyan"},{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","first_name":"Aglaja","orcid":"0000-0002-2187-6656","full_name":"Kopf, Aglaja","last_name":"Kopf"},{"last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"last_name":"Zheden","orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa","first_name":"Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","last_name":"Kaufmann"},{"last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"quality_controlled":"1","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"abstract":[{"lang":"eng","text":"When crawling through the body, leukocytes often traverse tissues that are densely packed with extracellular matrix and other cells, and this raises the question: How do leukocytes overcome compressive mechanical loads? Here, we show that the actin cortex of leukocytes is mechanoresponsive and that this responsiveness requires neither force sensing via the nucleus nor adhesive interactions with a substrate. Upon global compression of the cell body as well as local indentation of the plasma membrane, Wiskott-Aldrich syndrome protein (WASp) assembles into dot-like structures, providing activation platforms for Arp2/3 nucleated actin patches. These patches locally push against the external load, which can be obstructing collagen fibers or other cells, and thereby create space to facilitate forward locomotion. We show in vitro and in vivo that this WASp function is rate limiting for ameboid leukocyte migration in dense but not in loose environments and is required for trafficking through diverse tissues such as skin and lymph nodes."}],"page":"47-62.e9","status":"public","article_processing_charge":"No","date_published":"2022-01-10T00:00:00Z","date_created":"2022-01-30T23:01:33Z","external_id":{"isi":["000768933800005"],"pmid":["34919802"]},"article_type":"original","related_material":{"record":[{"status":"public","id":"20149","relation":"dissertation_contains"},{"status":"public","id":"12726","relation":"dissertation_contains"},{"id":"14530","status":"public","relation":"dissertation_contains"},{"status":"public","id":"12401","relation":"dissertation_contains"}]},"date_updated":"2026-05-30T22:31:08Z","_id":"10703","month":"01","title":"WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues","ddc":["570"],"corr_author":"1","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"pmid":1,"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","type":"journal_article","ec_funded":1,"volume":57,"scopus_import":"1","project":[{"grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"},{"name":"Cellular Navigation Along Spatial Gradients","grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"main_file_link":[{"open_access":"1","url":"https://www.sciencedirect.com/science/article/pii/S1534580721009497"}],"oa_version":"Published Version","day":"10","doi":"10.1016/j.devcel.2021.11.024","oa":1,"citation":{"ama":"Gaertner F, Dos Reis Rodrigues P, de Vries I, et al. WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. Developmental Cell. 2022;57(1):47-62.e9. doi:10.1016/j.devcel.2021.11.024","short":"F. Gaertner, P. Dos Reis Rodrigues, I. de Vries, M. Hons, J. Aguilera, M. Riedl, A.F. Leithner, S. Tasciyan, A. Kopf, J. Merrin, V. Zheden, W. Kaufmann, R. Hauschild, M.K. Sixt, Developmental Cell 57 (2022) 47–62.e9.","chicago":"Gaertner, Florian, Patricia Dos Reis Rodrigues, Ingrid de Vries, Miroslav Hons, Juan Aguilera, Michael Riedl, Alexander F Leithner, et al. “WASp Triggers Mechanosensitive Actin Patches to Facilitate Immune Cell Migration in Dense Tissues.” Developmental Cell. Cell Press, 2022. https://doi.org/10.1016/j.devcel.2021.11.024.","ieee":"F. Gaertner et al., “WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues,” Developmental Cell, vol. 57, no. 1. Cell Press, p. 47–62.e9, 2022.","ista":"Gaertner F, Dos Reis Rodrigues P, de Vries I, Hons M, Aguilera J, Riedl M, Leithner AF, Tasciyan S, Kopf A, Merrin J, Zheden V, Kaufmann W, Hauschild R, Sixt MK. 2022. WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. Developmental Cell. 57(1), 47–62.e9.","apa":"Gaertner, F., Dos Reis Rodrigues, P., de Vries, I., Hons, M., Aguilera, J., Riedl, M., … Sixt, M. K. (2022). WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2021.11.024","mla":"Gaertner, Florian, et al. “WASp Triggers Mechanosensitive Actin Patches to Facilitate Immune Cell Migration in Dense Tissues.” Developmental Cell, vol. 57, no. 1, Cell Press, 2022, p. 47–62.e9, doi:10.1016/j.devcel.2021.11.024."},"publisher":"Cell Press","publication_status":"published","department":[{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"},{"_id":"BjHo"}],"intvolume":" 57"},{"date_updated":"2025-12-15T10:01:56Z","_id":"11052","month":"11","title":"Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"type":"journal_article","scopus_import":"1","volume":56,"oa_version":"None","day":"08","doi":"10.1016/j.devcel.2021.10.008","citation":{"chicago":"Krishna, Shefali, Rafael Arrojo e Drigo, Juliana S. Capitanio, Ranjan Ramachandra, Mark Ellisman, and Martin Hetzer. “Identification of Long-Lived Proteins in the Mitochondria Reveals Increased Stability of the Electron Transport Chain.” Developmental Cell. Elsevier, 2021. https://doi.org/10.1016/j.devcel.2021.10.008.","ama":"Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer M. Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. Developmental Cell. 2021;56(21):P2952-2965.e9. doi:10.1016/j.devcel.2021.10.008","short":"S. Krishna, R. Arrojo e Drigo, J.S. Capitanio, R. Ramachandra, M. Ellisman, M. Hetzer, Developmental Cell 56 (2021) P2952–2965.e9.","mla":"Krishna, Shefali, et al. “Identification of Long-Lived Proteins in the Mitochondria Reveals Increased Stability of the Electron Transport Chain.” Developmental Cell, vol. 56, no. 21, Elsevier, 2021, p. P2952–2965.e9, doi:10.1016/j.devcel.2021.10.008.","ieee":"S. Krishna, R. Arrojo e Drigo, J. S. Capitanio, R. Ramachandra, M. Ellisman, and M. Hetzer, “Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain,” Developmental Cell, vol. 56, no. 21. Elsevier, p. P2952–2965.e9, 2021.","apa":"Krishna, S., Arrojo e Drigo, R., Capitanio, J. S., Ramachandra, R., Ellisman, M., & Hetzer, M. (2021). Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2021.10.008","ista":"Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer M. 2021. Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. Developmental Cell. 56(21), P2952–2965.e9."},"publisher":"Elsevier","publication_status":"published","department":[{"_id":"MaHe"}],"keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"intvolume":" 56","year":"2021","language":[{"iso":"eng"}],"publication":"Developmental Cell","extern":"1","publication_identifier":{"issn":["1534-5807"]},"author":[{"full_name":"Krishna, Shefali","first_name":"Shefali","last_name":"Krishna"},{"first_name":"Rafael","full_name":"Arrojo e Drigo, Rafael","last_name":"Arrojo e Drigo"},{"full_name":"Capitanio, Juliana S.","first_name":"Juliana S.","last_name":"Capitanio"},{"full_name":"Ramachandra, Ranjan","first_name":"Ranjan","last_name":"Ramachandra"},{"full_name":"Ellisman, Mark","first_name":"Mark","last_name":"Ellisman"},{"last_name":"HETZER","orcid":"0000-0002-2111-992X","full_name":"HETZER, Martin W","first_name":"Martin W","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed"}],"issue":"21","quality_controlled":"1","abstract":[{"lang":"eng","text":"In order to combat molecular damage, most cellular proteins undergo rapid turnover. We have previously identified large nuclear protein assemblies that can persist for years in post-mitotic tissues and are subject to age-related decline. Here, we report that mitochondria can be long lived in the mouse brain and reveal that specific mitochondrial proteins have half-lives longer than the average proteome. These mitochondrial long-lived proteins (mitoLLPs) are core components of the electron transport chain (ETC) and display increased longevity in respiratory supercomplexes. We find that COX7C, a mitoLLP that forms a stable contact site between complexes I and IV, is required for complex IV and supercomplex assembly. Remarkably, even upon depletion of COX7C transcripts, ETC function is maintained for days, effectively uncoupling mitochondrial function from ongoing transcription of its mitoLLPs. Our results suggest that modulating protein longevity within the ETC is critical for mitochondrial proteome maintenance and the robustness of mitochondrial function."}],"page":"P2952-2965.e9","status":"public","article_processing_charge":"No","date_created":"2022-04-07T07:43:14Z","date_published":"2021-11-08T00:00:00Z","external_id":{"pmid":["34715012"]},"article_type":"original"},{"language":[{"iso":"eng"}],"publication":"Developmental Cell","year":"2021","isi":1,"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"issue":"6","author":[{"orcid":"0000-0001-6120-3723","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","last_name":"Gärtner"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt"}],"quality_controlled":"1","page":"723-725","abstract":[{"lang":"eng","text":"In this issue of Developmental Cell, Doyle and colleagues identify periodic anterior contraction as a characteristic feature of fibroblasts and mesenchymal cancer cells embedded in 3D collagen gels. This contractile mechanism generates a matrix prestrain required for crawling in fibrous 3D environments."}],"article_type":"original","external_id":{"pmid":["33756118"],"isi":["000631681200004"]},"status":"public","date_created":"2021-03-28T22:01:41Z","date_published":"2021-03-22T00:00:00Z","article_processing_charge":"No","title":"Engaging the front wheels to drive through fibrous terrain","corr_author":"1","_id":"9294","date_updated":"2025-07-10T12:01:41Z","month":"03","pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","doi":"10.1016/j.devcel.2021.03.002","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2021.03.002","open_access":"1"}],"oa_version":"Published Version","day":"22","volume":56,"scopus_import":"1","department":[{"_id":"MiSi"}],"publication_status":"published","intvolume":" 56","citation":{"ista":"Gärtner FR, Sixt MK. 2021. Engaging the front wheels to drive through fibrous terrain. Developmental Cell. 56(6), 723–725.","ieee":"F. R. Gärtner and M. K. Sixt, “Engaging the front wheels to drive through fibrous terrain,” Developmental Cell, vol. 56, no. 6. Elsevier, pp. 723–725, 2021.","apa":"Gärtner, F. R., & Sixt, M. K. (2021). Engaging the front wheels to drive through fibrous terrain. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2021.03.002","mla":"Gärtner, Florian R., and Michael K. Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” Developmental Cell, vol. 56, no. 6, Elsevier, 2021, pp. 723–25, doi:10.1016/j.devcel.2021.03.002.","short":"F.R. Gärtner, M.K. Sixt, Developmental Cell 56 (2021) 723–725.","ama":"Gärtner FR, Sixt MK. Engaging the front wheels to drive through fibrous terrain. Developmental Cell. 2021;56(6):723-725. doi:10.1016/j.devcel.2021.03.002","chicago":"Gärtner, Florian R, and Michael K Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” Developmental Cell. Elsevier, 2021. https://doi.org/10.1016/j.devcel.2021.03.002."},"oa":1,"publisher":"Elsevier"},{"volume":56,"scopus_import":"1","doi":"10.1016/j.devcel.2020.12.002","oa_version":"Published Version","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2020.12.002","open_access":"1"}],"day":"25","citation":{"chicago":"Shamipour, Shayan, Silvia Caballero Mancebo, and Carl-Philipp J Heisenberg. “Cytoplasm’s Got Moves.” Developmental Cell. Elsevier, 2021. https://doi.org/10.1016/j.devcel.2020.12.002.","short":"S. Shamipour, S. Caballero Mancebo, C.-P.J. Heisenberg, Developmental Cell 56 (2021) P213-226.","ama":"Shamipour S, Caballero Mancebo S, Heisenberg C-PJ. Cytoplasm’s got moves. Developmental Cell. 2021;56(2):P213-226. doi:10.1016/j.devcel.2020.12.002","mla":"Shamipour, Shayan, et al. “Cytoplasm’s Got Moves.” Developmental Cell, vol. 56, no. 2, Elsevier, 2021, pp. P213-226, doi:10.1016/j.devcel.2020.12.002.","apa":"Shamipour, S., Caballero Mancebo, S., & Heisenberg, C.-P. J. (2021). Cytoplasm’s got moves. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2020.12.002","ista":"Shamipour S, Caballero Mancebo S, Heisenberg C-PJ. 2021. Cytoplasm’s got moves. Developmental Cell. 56(2), P213-226.","ieee":"S. Shamipour, S. Caballero Mancebo, and C.-P. J. Heisenberg, “Cytoplasm’s got moves,” Developmental Cell, vol. 56, no. 2. Elsevier, pp. P213-226, 2021."},"oa":1,"publisher":"Elsevier","department":[{"_id":"CaHe"}],"publication_status":"published","intvolume":" 56","_id":"9006","date_updated":"2026-05-30T22:30:52Z","month":"01","title":"Cytoplasm's got moves","corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"type":"journal_article","page":"P213-226","abstract":[{"lang":"eng","text":"Cytoplasm is a gel-like crowded environment composed of various macromolecules, organelles, cytoskeletal networks, and cytosol. The structure of the cytoplasm is highly organized and heterogeneous due to the crowding of its constituents and their effective compartmentalization. In such an environment, the diffusive dynamics of the molecules are restricted, an effect that is further amplified by clustering and anchoring of molecules. Despite the crowded nature of the cytoplasm at the microscopic scale, large-scale reorganization of the cytoplasm is essential for important cellular functions, such as cell division and polarization. How such mesoscale reorganization of the cytoplasm is achieved, especially for large cells such as oocytes or syncytial tissues that can span hundreds of micrometers in size, is only beginning to be understood. In this review, we will discuss recent advances in elucidating the molecular, cellular, and biophysical mechanisms by which the cytoskeleton drives cytoplasmic reorganization across different scales, structures, and species."}],"status":"public","date_created":"2021-01-17T23:01:10Z","date_published":"2021-01-25T00:00:00Z","article_processing_charge":"No","article_type":"original","external_id":{"isi":["000613273900009"],"pmid":["33321104"]},"related_material":{"record":[{"relation":"dissertation_contains","id":"9623","status":"public"}]},"isi":1,"year":"2021","language":[{"iso":"eng"}],"publication":"Developmental Cell","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"acknowledgement":"We would like to thank Justine Renno for illustrations and Edouard Hannezo and members of the Heisenberg group for their comments on previous versions of the manuscript.","author":[{"first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan","last_name":"Shamipour"},{"first_name":"Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","full_name":"Caballero Mancebo, Silvia","orcid":"0000-0002-5223-3346","last_name":"Caballero Mancebo"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"issue":"2","quality_controlled":"1"},{"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"isi":1,"year":"2020","language":[{"iso":"eng"}],"publication":"Developmental Cell","file_date_updated":"2021-02-04T10:20:02Z","acknowledgement":"This work was supported by the Medical Research Council UK (MRC Program award MC_UU_12018/5 ), the European Research Council (starting grant 311637 -MorphoCorDiv and consolidator grant 820188 -NanoMechShape to E.K.P.), and the Leverhulme Trust (Leverhulme Prize in Biological Sciences to E.K.P.). K.J.C. acknowledges support from the Royal Society (Royal Society Research Fellowship). A.C. acknowledges support from EMBO ( ALTF 2015-563 ), the Wellcome Trust ( 201334/Z/16/Z ), and the Fondation Bettencourt-Schueller (Prix Jeune Chercheur, 2015).","quality_controlled":"1","issue":"2","author":[{"first_name":"Agathe","full_name":"Chaigne, Agathe","last_name":"Chaigne"},{"last_name":"Labouesse","first_name":"Céline","full_name":"Labouesse, Céline"},{"last_name":"White","full_name":"White, Ian J.","first_name":"Ian J."},{"full_name":"Agnew, Meghan","first_name":"Meghan","last_name":"Agnew"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo"},{"last_name":"Chalut","first_name":"Kevin J.","full_name":"Chalut, Kevin J."},{"last_name":"Paluch","first_name":"Ewa K.","full_name":"Paluch, Ewa K."}],"file":[{"date_updated":"2021-02-04T10:20:02Z","file_size":6929686,"file_id":"9086","success":1,"access_level":"open_access","creator":"dernst","content_type":"application/pdf","checksum":"88e1a031a61689165d19a19c2f16d795","file_name":"2020_DevelopmCell_Chaigne.pdf","date_created":"2021-02-04T10:20:02Z","relation":"main_file"}],"page":"195-208","abstract":[{"text":"Cell fate transitions are key to development and homeostasis. It is thus essential to understand the cellular mechanisms controlling fate transitions. Cell division has been implicated in fate decisions in many stem cell types, including neuronal and epithelial progenitors. In other stem cells, such as embryonic stem (ES) cells, the role of division remains unclear. Here, we show that exit from naive pluripotency in mouse ES cells generally occurs after a division. We further show that exit timing is strongly correlated between sister cells, which remain connected by cytoplasmic bridges long after division, and that bridge abscission progressively accelerates as cells exit naive pluripotency. Finally, interfering with abscission impairs naive pluripotency exit, and artificially inducing abscission accelerates it. Altogether, our data indicate that a switch in the division machinery leading to faster abscission regulates pluripotency exit. Our study identifies abscission as a key cellular process coupling cell division to fate transitions.","lang":"eng"}],"date_created":"2020-10-18T22:01:37Z","date_published":"2020-10-26T00:00:00Z","article_processing_charge":"No","status":"public","article_type":"original","external_id":{"isi":["000582501100012"],"pmid":["32979313"]},"month":"10","_id":"8672","date_updated":"2025-07-10T11:57:15Z","title":"Abscission couples cell division to embryonic stem cell fate","ddc":["570"],"type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"scopus_import":"1","volume":55,"has_accepted_license":"1","doi":"10.1016/j.devcel.2020.09.001","oa_version":"Published Version","day":"26","publisher":"Elsevier","citation":{"mla":"Chaigne, Agathe, et al. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” Developmental Cell, vol. 55, no. 2, Elsevier, 2020, pp. 195–208, doi:10.1016/j.devcel.2020.09.001.","ieee":"A. Chaigne et al., “Abscission couples cell division to embryonic stem cell fate,” Developmental Cell, vol. 55, no. 2. Elsevier, pp. 195–208, 2020.","ista":"Chaigne A, Labouesse C, White IJ, Agnew M, Hannezo EB, Chalut KJ, Paluch EK. 2020. Abscission couples cell division to embryonic stem cell fate. Developmental Cell. 55(2), 195–208.","apa":"Chaigne, A., Labouesse, C., White, I. J., Agnew, M., Hannezo, E. B., Chalut, K. J., & Paluch, E. K. (2020). Abscission couples cell division to embryonic stem cell fate. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2020.09.001","ama":"Chaigne A, Labouesse C, White IJ, et al. Abscission couples cell division to embryonic stem cell fate. Developmental Cell. 2020;55(2):195-208. doi:10.1016/j.devcel.2020.09.001","short":"A. Chaigne, C. Labouesse, I.J. White, M. Agnew, E.B. Hannezo, K.J. Chalut, E.K. Paluch, Developmental Cell 55 (2020) 195–208.","chicago":"Chaigne, Agathe, Céline Labouesse, Ian J. White, Meghan Agnew, Edouard B Hannezo, Kevin J. Chalut, and Ewa K. Paluch. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” Developmental Cell. Elsevier, 2020. https://doi.org/10.1016/j.devcel.2020.09.001."},"oa":1,"intvolume":" 55","department":[{"_id":"EdHa"}],"publication_status":"published"}]