---
_id: '603'
abstract:
- lang: eng
  text: During transcription, RNA polymerase II (Pol II) associates with the conserved
    elongation factor DSIF. DSIF renders the elongation complex stable and functions
    during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography
    to determine the structure of the mammalian Pol II-DSIF elongation complex at
    a nominal resolution of 3.4. Human DSIF has a modular structure with two domains
    forming a DNA clamp, two domains forming an RNA clamp, and one domain buttressing
    the RNA clamp. The clamps maintain the transcription bubble, position upstream
    DNA, and retain the RNA transcript in the exit tunnel. The mobile C-terminal region
    of DSIF is located near exiting RNA, where it can recruit factors for RNA processing.
    The structure provides insight into the roles of DSIF during mRNA synthesis.
article_processing_charge: No
author:
- first_name: Carrie A
  full_name: Bernecky, Carrie A
  id: 2CB9DFE2-F248-11E8-B48F-1D18A9856A87
  last_name: Bernecky
  orcid: 0000-0003-0893-7036
- first_name: Jürgen
  full_name: Plitzko, Jürgen
  last_name: Plitzko
- first_name: Patrick
  full_name: Cramer, Patrick
  last_name: Cramer
citation:
  ama: Bernecky C, Plitzko J, Cramer P. Structure of a transcribing RNA polymerase
    II-DSIF complex reveals a multidentate DNA-RNA clamp. <i>Nature Structural and
    Molecular Biology</i>. 2017;24(10):809-815. doi:<a href="https://doi.org/10.1038/nsmb.3465">10.1038/nsmb.3465</a>
  apa: Bernecky, C., Plitzko, J., &#38; Cramer, P. (2017). Structure of a transcribing
    RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp. <i>Nature
    Structural and Molecular Biology</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/nsmb.3465">https://doi.org/10.1038/nsmb.3465</a>
  chicago: Bernecky, Carrie, Jürgen Plitzko, and Patrick Cramer. “Structure of a Transcribing
    RNA Polymerase II-DSIF Complex Reveals a Multidentate DNA-RNA Clamp.” <i>Nature
    Structural and Molecular Biology</i>. Nature Publishing Group, 2017. <a href="https://doi.org/10.1038/nsmb.3465">https://doi.org/10.1038/nsmb.3465</a>.
  ieee: C. Bernecky, J. Plitzko, and P. Cramer, “Structure of a transcribing RNA polymerase
    II-DSIF complex reveals a multidentate DNA-RNA clamp,” <i>Nature Structural and
    Molecular Biology</i>, vol. 24, no. 10. Nature Publishing Group, pp. 809–815,
    2017.
  ista: Bernecky C, Plitzko J, Cramer P. 2017. Structure of a transcribing RNA polymerase
    II-DSIF complex reveals a multidentate DNA-RNA clamp. Nature Structural and Molecular
    Biology. 24(10), 809–815.
  mla: Bernecky, Carrie, et al. “Structure of a Transcribing RNA Polymerase II-DSIF
    Complex Reveals a Multidentate DNA-RNA Clamp.” <i>Nature Structural and Molecular
    Biology</i>, vol. 24, no. 10, Nature Publishing Group, 2017, pp. 809–15, doi:<a
    href="https://doi.org/10.1038/nsmb.3465">10.1038/nsmb.3465</a>.
  short: C. Bernecky, J. Plitzko, P. Cramer, Nature Structural and Molecular Biology
    24 (2017) 809–815.
date_created: 2018-12-11T11:47:26Z
date_published: 2017-10-05T00:00:00Z
date_updated: 2025-09-11T07:37:28Z
day: '05'
doi: 10.1038/nsmb.3465
extern: '1'
external_id:
  isi:
  - '000412278000007'
intvolume: '        24'
isi: 1
issue: '10'
language:
- iso: eng
month: '10'
oa_version: None
page: 809 - 815
publication: Nature Structural and Molecular Biology
publication_identifier:
  issn:
  - '15459993'
publication_status: published
publisher: Nature Publishing Group
publist_id: '7202'
quality_controlled: '1'
status: public
title: Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate
  DNA-RNA clamp
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 24
year: '2017'
...