---
OA_type: closed access
_id: '19470'
abstract:
- lang: eng
  text: When food is freely available, eating occurs without energy deficit. While
    agouti-related peptide (AgRP) neurons are likely involved, their activation is
    thought to require negative energy balance. To investigate this, we implemented
    long-term, continuous in vivo fiber-photometry recordings in mice. We discovered
    new forms of AgRP neuron regulation, including fast pre-ingestive decreases in
    activity and unexpectedly rapid activation by fasting. Furthermore, AgRP neuron
    activity has a circadian rhythm that peaks concurrent with the daily feeding onset.
    Importantly, this rhythm persists when nutrition is provided via constant-rate
    gastric infusions. Hence, it is not secondary to a circadian feeding rhythm. The
    AgRP neuron rhythm is driven by the circadian clock, the suprachiasmatic nucleus
    (SCN), as SCN ablation abolishes the circadian rhythm in AgRP neuron activity
    and feeding. The SCN activates AgRP neurons via excitatory afferents from thyrotrophin-releasing
    hormone-expressing neurons in the dorsomedial hypothalamus (DMHTrh neurons) to
    drive daily feeding rhythms.
article_processing_charge: No
article_type: original
author:
- first_name: Amelia May Barnett
  full_name: Douglass, Amelia May Barnett
  id: de5f6fda-80fb-11ef-996f-a8c4ecd8e289
  last_name: Douglass
  orcid: 0000-0001-5398-6473
- first_name: Hakan
  full_name: Kucukdereli, Hakan
  last_name: Kucukdereli
- first_name: Joseph C.
  full_name: Madara, Joseph C.
  last_name: Madara
- first_name: Daqing
  full_name: Wang, Daqing
  last_name: Wang
- first_name: Chen
  full_name: Wu, Chen
  last_name: Wu
- first_name: Elijah D.
  full_name: Lowenstein, Elijah D.
  last_name: Lowenstein
- first_name: Jenkang
  full_name: Tao, Jenkang
  last_name: Tao
- first_name: Bradford B.
  full_name: Lowell, Bradford B.
  last_name: Lowell
citation:
  ama: Douglass AM, Kucukdereli H, Madara JC, et al. Acute and circadian feedforward
    regulation of agouti-related peptide hunger neurons. <i>Cell Metabolism</i>. 2024;37(3):708-722.e5.
    doi:<a href="https://doi.org/10.1016/j.cmet.2024.11.009">10.1016/j.cmet.2024.11.009</a>
  apa: Douglass, A. M., Kucukdereli, H., Madara, J. C., Wang, D., Wu, C., Lowenstein,
    E. D., … Lowell, B. B. (2024). Acute and circadian feedforward regulation of agouti-related
    peptide hunger neurons. <i>Cell Metabolism</i>. Elsevier. <a href="https://doi.org/10.1016/j.cmet.2024.11.009">https://doi.org/10.1016/j.cmet.2024.11.009</a>
  chicago: Douglass, Amelia M., Hakan Kucukdereli, Joseph C. Madara, Daqing Wang,
    Chen Wu, Elijah D. Lowenstein, Jenkang Tao, and Bradford B. Lowell. “Acute and
    Circadian Feedforward Regulation of Agouti-Related Peptide Hunger Neurons.” <i>Cell
    Metabolism</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.cmet.2024.11.009">https://doi.org/10.1016/j.cmet.2024.11.009</a>.
  ieee: A. M. Douglass <i>et al.</i>, “Acute and circadian feedforward regulation
    of agouti-related peptide hunger neurons,” <i>Cell Metabolism</i>, vol. 37, no.
    3. Elsevier, p. 708–722.e5, 2024.
  ista: Douglass AM, Kucukdereli H, Madara JC, Wang D, Wu C, Lowenstein ED, Tao J,
    Lowell BB. 2024. Acute and circadian feedforward regulation of agouti-related
    peptide hunger neurons. Cell Metabolism. 37(3), 708–722.e5.
  mla: Douglass, Amelia M., et al. “Acute and Circadian Feedforward Regulation of
    Agouti-Related Peptide Hunger Neurons.” <i>Cell Metabolism</i>, vol. 37, no. 3,
    Elsevier, 2024, p. 708–722.e5, doi:<a href="https://doi.org/10.1016/j.cmet.2024.11.009">10.1016/j.cmet.2024.11.009</a>.
  short: A.M. Douglass, H. Kucukdereli, J.C. Madara, D. Wang, C. Wu, E.D. Lowenstein,
    J. Tao, B.B. Lowell, Cell Metabolism 37 (2024) 708–722.e5.
date_created: 2025-04-03T12:27:39Z
date_published: 2024-03-04T00:00:00Z
date_updated: 2025-07-10T11:51:40Z
day: '04'
doi: 10.1016/j.cmet.2024.11.009
extern: '1'
external_id:
  pmid:
  - '39719709'
intvolume: '        37'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 708-722.e5
pmid: 1
publication: Cell Metabolism
publication_identifier:
  issn:
  - 1550-4131
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Acute and circadian feedforward regulation of agouti-related peptide hunger
  neurons
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 37
year: '2024'
...
---
_id: '11062'
abstract:
- lang: eng
  text: Most neurons are not replaced during an animal’s lifetime. This nondividing
    state is characterized by extreme longevity and age-dependent decline of key regulatory
    proteins. To study the lifespans of cells and proteins in adult tissues, we combined
    isotope labeling of mice with a hybrid imaging method (MIMS-EM). Using 15N mapping,
    we show that liver and pancreas are composed of cells with vastly different ages,
    many as old as the animal. Strikingly, we also found that a subset of fibroblasts
    and endothelial cells, both known for their replicative potential, are characterized
    by the absence of cell division during adulthood. In addition, we show that the
    primary cilia of beta cells and neurons contains different structural regions
    with vastly different lifespans. Based on these results, we propose that age mosaicism
    across multiple scales is a fundamental principle of adult tissue, cell, and protein
    complex organization.
article_processing_charge: No
article_type: original
author:
- first_name: Rafael
  full_name: Arrojo e Drigo, Rafael
  last_name: Arrojo e Drigo
- first_name: Varda
  full_name: Lev-Ram, Varda
  last_name: Lev-Ram
- first_name: Swati
  full_name: Tyagi, Swati
  last_name: Tyagi
- first_name: Ranjan
  full_name: Ramachandra, Ranjan
  last_name: Ramachandra
- first_name: Thomas
  full_name: Deerinck, Thomas
  last_name: Deerinck
- first_name: Eric
  full_name: Bushong, Eric
  last_name: Bushong
- first_name: Sebastien
  full_name: Phan, Sebastien
  last_name: Phan
- first_name: Victoria
  full_name: Orphan, Victoria
  last_name: Orphan
- first_name: Claude
  full_name: Lechene, Claude
  last_name: Lechene
- first_name: Mark H.
  full_name: Ellisman, Mark H.
  last_name: Ellisman
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: Arrojo e Drigo R, Lev-Ram V, Tyagi S, et al. Age mosaicism across multiple
    scales in adult tissues. <i>Cell Metabolism</i>. 2019;30(2):343-351.e3. doi:<a
    href="https://doi.org/10.1016/j.cmet.2019.05.010">10.1016/j.cmet.2019.05.010</a>
  apa: Arrojo e Drigo, R., Lev-Ram, V., Tyagi, S., Ramachandra, R., Deerinck, T.,
    Bushong, E., … Hetzer, M. (2019). Age mosaicism across multiple scales in adult
    tissues. <i>Cell Metabolism</i>. Elsevier. <a href="https://doi.org/10.1016/j.cmet.2019.05.010">https://doi.org/10.1016/j.cmet.2019.05.010</a>
  chicago: Arrojo e Drigo, Rafael, Varda Lev-Ram, Swati Tyagi, Ranjan Ramachandra,
    Thomas Deerinck, Eric Bushong, Sebastien Phan, et al. “Age Mosaicism across Multiple
    Scales in Adult Tissues.” <i>Cell Metabolism</i>. Elsevier, 2019. <a href="https://doi.org/10.1016/j.cmet.2019.05.010">https://doi.org/10.1016/j.cmet.2019.05.010</a>.
  ieee: R. Arrojo e Drigo <i>et al.</i>, “Age mosaicism across multiple scales in
    adult tissues,” <i>Cell Metabolism</i>, vol. 30, no. 2. Elsevier, p. 343–351.e3,
    2019.
  ista: Arrojo e Drigo R, Lev-Ram V, Tyagi S, Ramachandra R, Deerinck T, Bushong E,
    Phan S, Orphan V, Lechene C, Ellisman MH, Hetzer M. 2019. Age mosaicism across
    multiple scales in adult tissues. Cell Metabolism. 30(2), 343–351.e3.
  mla: Arrojo e Drigo, Rafael, et al. “Age Mosaicism across Multiple Scales in Adult
    Tissues.” <i>Cell Metabolism</i>, vol. 30, no. 2, Elsevier, 2019, p. 343–351.e3,
    doi:<a href="https://doi.org/10.1016/j.cmet.2019.05.010">10.1016/j.cmet.2019.05.010</a>.
  short: R. Arrojo e Drigo, V. Lev-Ram, S. Tyagi, R. Ramachandra, T. Deerinck, E.
    Bushong, S. Phan, V. Orphan, C. Lechene, M.H. Ellisman, M. Hetzer, Cell Metabolism
    30 (2019) 343–351.e3.
date_created: 2022-04-07T07:45:21Z
date_published: 2019-08-06T00:00:00Z
date_updated: 2025-12-15T10:02:11Z
day: '06'
department:
- _id: MaHe
doi: 10.1016/j.cmet.2019.05.010
extern: '1'
external_id:
  pmid:
  - '31178361'
intvolume: '        30'
issue: '2'
keyword:
- Cell Biology
- Molecular Biology
- Physiology
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.cmet.2019.05.010
month: '08'
oa: 1
oa_version: Published Version
page: 343-351.e3
pmid: 1
publication: Cell Metabolism
publication_identifier:
  issn:
  - 1550-4131
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Age mosaicism across multiple scales in adult tissues
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 30
year: '2019'
...
---
_id: '6145'
author:
- first_name: Merav
  full_name: Cohen, Merav
  last_name: Cohen
- first_name: Vincenzina
  full_name: Reale, Vincenzina
  last_name: Reale
- first_name: Birgitta
  full_name: Olofsson, Birgitta
  last_name: Olofsson
- first_name: Andrew
  full_name: Knights, Andrew
  last_name: Knights
- first_name: Peter
  full_name: Evans, Peter
  last_name: Evans
- first_name: Mario
  full_name: de Bono, Mario
  id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
  last_name: de Bono
  orcid: 0000-0001-8347-0443
citation:
  ama: Cohen M, Reale V, Olofsson B, Knights A, Evans P, de Bono M. Coordinated regulation
    of foraging and metabolism in C. elegans by RFamide neuropeptide signaling. <i>Cell
    Metabolism</i>. 2009;9(4):375-385. doi:<a href="https://doi.org/10.1016/j.cmet.2009.02.003">10.1016/j.cmet.2009.02.003</a>
  apa: Cohen, M., Reale, V., Olofsson, B., Knights, A., Evans, P., &#38; de Bono,
    M. (2009). Coordinated regulation of foraging and metabolism in C. elegans by
    RFamide neuropeptide signaling. <i>Cell Metabolism</i>. Elsevier. <a href="https://doi.org/10.1016/j.cmet.2009.02.003">https://doi.org/10.1016/j.cmet.2009.02.003</a>
  chicago: Cohen, Merav, Vincenzina Reale, Birgitta Olofsson, Andrew Knights, Peter
    Evans, and Mario de Bono. “Coordinated Regulation of Foraging and Metabolism in
    C. Elegans by RFamide Neuropeptide Signaling.” <i>Cell Metabolism</i>. Elsevier,
    2009. <a href="https://doi.org/10.1016/j.cmet.2009.02.003">https://doi.org/10.1016/j.cmet.2009.02.003</a>.
  ieee: M. Cohen, V. Reale, B. Olofsson, A. Knights, P. Evans, and M. de Bono, “Coordinated
    regulation of foraging and metabolism in C. elegans by RFamide neuropeptide signaling,”
    <i>Cell Metabolism</i>, vol. 9, no. 4. Elsevier, pp. 375–385, 2009.
  ista: Cohen M, Reale V, Olofsson B, Knights A, Evans P, de Bono M. 2009. Coordinated
    regulation of foraging and metabolism in C. elegans by RFamide neuropeptide signaling.
    Cell Metabolism. 9(4), 375–385.
  mla: Cohen, Merav, et al. “Coordinated Regulation of Foraging and Metabolism in
    C. Elegans by RFamide Neuropeptide Signaling.” <i>Cell Metabolism</i>, vol. 9,
    no. 4, Elsevier, 2009, pp. 375–85, doi:<a href="https://doi.org/10.1016/j.cmet.2009.02.003">10.1016/j.cmet.2009.02.003</a>.
  short: M. Cohen, V. Reale, B. Olofsson, A. Knights, P. Evans, M. de Bono, Cell Metabolism
    9 (2009) 375–385.
date_created: 2019-03-21T07:57:52Z
date_published: 2009-04-08T00:00:00Z
date_updated: 2021-01-12T08:06:20Z
day: '08'
doi: 10.1016/j.cmet.2009.02.003
extern: '1'
external_id:
  pmid:
  - '19356718'
intvolume: '         9'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 375-385
pmid: 1
publication: Cell Metabolism
publication_identifier:
  issn:
  - 1550-4131
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Coordinated regulation of foraging and metabolism in C. elegans by RFamide
  neuropeptide signaling
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2009'
...