---
APC_amount: 2236,02 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19566'
abstract:
- lang: eng
  text: "Purpose: Optic nerve crush (ONC) is a model for studying optic nerve trauma.
    Unilateral ONC induces massive retinal ganglion cell (RGC) degeneration in the
    affected eye, leading to vision loss within a month. A common assumption has been
    that the non-injured contralateral eye is unaffected due to the minimal retino-retinal
    projections of the RGCs at the chiasm. Yet, recently, microglia, the brain-resident
    macrophages, have shown a responsive phenotype in the contralateral eye after
    ONC. Whether RGC loss accompanies this phenotype is still controversial.\r\n\r\nMethods:
    Using the available RGCode algorithm and developing our own RGC-Quant deep-learning-based
    tool, we quantify RGC's total number and density across the entire retina after
    ONC.\r\n\r\nResults: We confirm a short-term microglia response in the contralateral
    eye after ONC, but this did not affect the microglia number. Furthermore, we cannot
    confirm the previously reported RGC loss between naïve and contralateral retinas
    5 weeks after ONC induction across the commonly used Cx3cr1creERT2 and C57BL6/J
    mouse models. Neither sex nor the direct comparison of the RGC markers Brn3a and
    RBPMS, with Brn3a co-labeling, on average, 89% of the RBPMS+-cells, explained
    this discrepancy, suggesting that the early microglia-responsive phenotype does
    not have immediate consequences on the RGC number.\r\n\r\nConclusions: Our results
    corroborate that unilateral optic nerve injury elicits a microglial response in
    the uninjured contralateral eye but without RGC loss. Therefore, the contralateral
    eye should be treated separately and not as an ONC control."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: PreCl
acknowledgement: "The authors thank the Scientific Service Units (SSU) of ISTA for
  the provided resources, specifically the Imaging and Optics Facility (IOF), the
  Lab Support Facility (LSF), and the Pre-Clinical Facility (PCF) team, specifically
  Sonja Haslinger, Claudia Gold, and Michael Schunn, for mouse colony management and
  support. We thank all members of the Siegert group for constant feedback on the
  project and the manuscript. \r\nSupported in whole or in part by the Austrian Science
  Fund (FWF) [10.55776/P37131]. For open access purposes, the author has applied a
  CC BY public copyright license to any author-accepted manuscript version arising
  from this submission. "
article_number: '49'
article_processing_charge: Yes
article_type: original
author:
- first_name: Florianne E
  full_name: Schoot Uiterkamp, Florianne E
  id: 3526230C-F248-11E8-B48F-1D18A9856A87
  last_name: Schoot Uiterkamp
- first_name: Margaret E
  full_name: Maes, Margaret E
  id: 3838F452-F248-11E8-B48F-1D18A9856A87
  last_name: Maes
  orcid: 0000-0001-9642-1085
- first_name: Mohammad
  full_name: Alamalhoda, Mohammad
  last_name: Alamalhoda
- first_name: Arsalan
  full_name: Firoozi, Arsalan
  last_name: Firoozi
- first_name: Gloria
  full_name: Colombo, Gloria
  id: 3483CF6C-F248-11E8-B48F-1D18A9856A87
  last_name: Colombo
  orcid: 0000-0001-9434-8902
- first_name: Sandra
  full_name: Siegert, Sandra
  id: 36ACD32E-F248-11E8-B48F-1D18A9856A87
  last_name: Siegert
  orcid: 0000-0001-8635-0877
citation:
  ama: Miteva FE, Maes ME, Alamalhoda M, Firoozi A, Colombo G, Siegert S. Optic nerve
    crush does not induce retinal ganglion cell loss in the contralateral eye. <i>Investigative
    Ophthalmology &#38; Visual Science</i>. 2025;66(3). doi:<a href="https://doi.org/10.1167/iovs.66.3.49">10.1167/iovs.66.3.49</a>
  apa: Miteva, F. E., Maes, M. E., Alamalhoda, M., Firoozi, A., Colombo, G., &#38;
    Siegert, S. (2025). Optic nerve crush does not induce retinal ganglion cell loss
    in the contralateral eye. <i>Investigative Ophthalmology &#38; Visual Science</i>.
    Association for Research in Vision and Ophthalmology. <a href="https://doi.org/10.1167/iovs.66.3.49">https://doi.org/10.1167/iovs.66.3.49</a>
  chicago: Miteva, Florianne E, Margaret E Maes, Mohammad Alamalhoda, Arsalan Firoozi,
    Gloria Colombo, and Sandra Siegert. “Optic Nerve Crush Does Not Induce Retinal
    Ganglion Cell Loss in the Contralateral Eye.” <i>Investigative Ophthalmology &#38;
    Visual Science</i>. Association for Research in Vision and Ophthalmology, 2025.
    <a href="https://doi.org/10.1167/iovs.66.3.49">https://doi.org/10.1167/iovs.66.3.49</a>.
  ieee: F. E. Miteva, M. E. Maes, M. Alamalhoda, A. Firoozi, G. Colombo, and S. Siegert,
    “Optic nerve crush does not induce retinal ganglion cell loss in the contralateral
    eye,” <i>Investigative Ophthalmology &#38; Visual Science</i>, vol. 66, no. 3.
    Association for Research in Vision and Ophthalmology, 2025.
  ista: Miteva FE, Maes ME, Alamalhoda M, Firoozi A, Colombo G, Siegert S. 2025. Optic
    nerve crush does not induce retinal ganglion cell loss in the contralateral eye.
    Investigative Ophthalmology &#38; Visual Science. 66(3), 49.
  mla: Miteva, Florianne E., et al. “Optic Nerve Crush Does Not Induce Retinal Ganglion
    Cell Loss in the Contralateral Eye.” <i>Investigative Ophthalmology &#38; Visual
    Science</i>, vol. 66, no. 3, 49, Association for Research in Vision and Ophthalmology,
    2025, doi:<a href="https://doi.org/10.1167/iovs.66.3.49">10.1167/iovs.66.3.49</a>.
  short: F.E. Miteva, M.E. Maes, M. Alamalhoda, A. Firoozi, G. Colombo, S. Siegert,
    Investigative Ophthalmology &#38; Visual Science 66 (2025).
corr_author: '1'
date_created: 2025-04-15T13:40:35Z
date_published: 2025-03-01T00:00:00Z
date_updated: 2026-05-20T06:37:12Z
day: '01'
ddc:
- '570'
department:
- _id: SaSi
doi: 10.1167/iovs.66.3.49
external_id:
  pmid:
  - '40126507'
file:
- access_level: open_access
  checksum: e8722ce5792f6c08fe1e191f7de6f147
  content_type: application/pdf
  creator: dernst
  date_created: 2025-04-15T13:49:10Z
  date_updated: 2025-04-15T13:49:10Z
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file_date_updated: 2025-04-15T13:49:10Z
fulldoi: https://doi.org/10.1167/iovs.66.3.49
has_accepted_license: '1'
intvolume: '        66'
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 7be82147-9f16-11ee-852c-f44682d73140
  grant_number: P37131
  name: Dissecting the morpho-functional relationship of microglia
- _id: 3AC91DDA-15DF-11EA-824D-93A3E7B544D1
  call_identifier: FWF
  name: FWF Open Access Fund
publication: Investigative Ophthalmology & Visual Science
publication_identifier:
  issn:
  - 1552-5783
publication_status: published
publisher: Association for Research in Vision and Ophthalmology
quality_controlled: '1'
related_material:
  link:
  - relation: software
    url: https://github.com/siegert-lab/RGC-Quant
  record:
  - id: '20467'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Optic nerve crush does not induce retinal ganglion cell loss in the contralateral
  eye
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 66
year: '2025'
...