---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21953'
abstract:
- lang: eng
text: Several Streptomyces strains were isolated from freshwater sediments collected
in the Laxenburg ponds (Lower Austria). Genome sequencing and bioinformatics analyses
revealed biosynthetic gene clusters (BGCs) that may specify production of chemically
diverse secondary metabolites. Various culture conditions were employed to induce
metabolite production, and subsequent LC-MS analyses facilitated the identification
of the produced compounds and their correlation with the corresponding BGCs. These
analyses of sediment-derived Streptomyces spp. highlight their extensive biosynthetic
potential, revealing a diverse range of bioactive secondary metabolites, including
siderophores, antibiotics, and other compounds with potential therapeutic applications.
Genomes of two Streptomyces isolates, one of them representing a potentially new
species, harbored several uncharacterized BGCs that may specify biosynthesis of
novel secondary metabolites. Although targeted overexpression of pathway-specific
regulators from these BGCs did not yield additional metabolites, whereas knockout
experiments led to metabolic changes, presumably reflecting regulatory or compensatory
interactions between multiple biosynthetic pathways. Continued exploration of
these strains and their BGCs may lead to the discovery of new bioactive molecules
with pharmaceutical and biotechnological applications.
acknowledgement: "The computational results of this work have been achieved using
the Life Science Compute Cluster (LiSC) of the University of Vienna. We additionally
thank Julia Ramesmayer for assistance during DNA extraction and sample preparation
for long-read sequencing. Support from the Mass Spectrometry Centre of the Faculty
of Chemistry, University of Vienna, is thankfully acknowledged.\r\nThe author(s)
declared that financial support was received for this work and/or its publication.
This work was supported by the University of Vienna via the Research Platform Secondary
Metabolomes of Bacterial Communities (MetaBac). Open access funding provided by
University of Vienna. "
article_number: '1793713'
article_processing_charge: Yes
article_type: original
author:
- first_name: Inmaculada
full_name: Tocino-Márquez, Inmaculada
last_name: Tocino-Márquez
- first_name: Martin
full_name: Zehl, Martin
id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55
last_name: Zehl
orcid: 0000-0001-9685-0373
- first_name: Jovana
full_name: Batajic, Jovana
last_name: Batajic
- first_name: Joana
full_name: Séneca, Joana
last_name: Séneca
- first_name: Petra
full_name: Pjevac, Petra
last_name: Pjevac
- first_name: José
full_name: Murillo-Alba, José
last_name: Murillo-Alba
- first_name: Jesús
full_name: Martín, Jesús
last_name: Martín
- first_name: Olga N.
full_name: Sekurova, Olga N.
last_name: Sekurova
- first_name: Sergey B.
full_name: Zotchev, Sergey B.
last_name: Zotchev
citation:
ama: Tocino-Márquez I, Zehl M, Batajic J, et al. Unveiling the genomes and secondary
metabolomes of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology.
2026;17. doi:10.3389/fmicb.2026.1793713
apa: Tocino-Márquez, I., Zehl, M., Batajic, J., Séneca, J., Pjevac, P., Murillo-Alba,
J., … Zotchev, S. B. (2026). Unveiling the genomes and secondary metabolomes of
Streptomyces spp. from freshwater sediments. Frontiers in Microbiology.
Frontiers Media. https://doi.org/10.3389/fmicb.2026.1793713
chicago: Tocino-Márquez, Inmaculada, Martin Zehl, Jovana Batajic, Joana Séneca,
Petra Pjevac, José Murillo-Alba, Jesús Martín, Olga N. Sekurova, and Sergey B.
Zotchev. “Unveiling the Genomes and Secondary Metabolomes of Streptomyces Spp.
from Freshwater Sediments.” Frontiers in Microbiology. Frontiers Media,
2026. https://doi.org/10.3389/fmicb.2026.1793713.
ieee: I. Tocino-Márquez et al., “Unveiling the genomes and secondary metabolomes
of Streptomyces spp. from freshwater sediments,” Frontiers in Microbiology,
vol. 17. Frontiers Media, 2026.
ista: Tocino-Márquez I, Zehl M, Batajic J, Séneca J, Pjevac P, Murillo-Alba J, Martín
J, Sekurova ON, Zotchev SB. 2026. Unveiling the genomes and secondary metabolomes
of Streptomyces spp. from freshwater sediments. Frontiers in Microbiology. 17,
1793713.
mla: Tocino-Márquez, Inmaculada, et al. “Unveiling the Genomes and Secondary Metabolomes
of Streptomyces Spp. from Freshwater Sediments.” Frontiers in Microbiology,
vol. 17, 1793713, Frontiers Media, 2026, doi:10.3389/fmicb.2026.1793713.
short: I. Tocino-Márquez, M. Zehl, J. Batajic, J. Séneca, P. Pjevac, J. Murillo-Alba,
J. Martín, O.N. Sekurova, S.B. Zotchev, Frontiers in Microbiology 17 (2026).
date_created: 2026-06-08T08:34:10Z
date_published: 2026-04-20T00:00:00Z
date_updated: 2026-06-10T07:49:04Z
day: '20'
ddc:
- '572'
department:
- _id: MassSpec
doi: 10.3389/fmicb.2026.1793713
external_id:
pmid:
- '42088272'
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pmid: 1
publication: Frontiers in Microbiology
publication_identifier:
issn:
- 1664-302X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
status: public
title: Unveiling the genomes and secondary metabolomes of Streptomyces spp. from freshwater
sediments
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legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
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