---
_id: '7350'
abstract:
- lang: eng
  text: The ability to sense environmental temperature and to coordinate growth and
    development accordingly, is critical to the reproductive success of plants. Flowering
    time is regulated at the level of gene expression by a complex network of factors
    that integrate environmental and developmental cues. One of the main players,
    involved in modulating flowering time in response to changes in ambient temperature
    is FLOWERING LOCUS M (FLM). FLM transcripts can undergo extensive alternative
    splicing producing multiple variants, of which FLM-β and FLM-δ are the most representative.
    While FLM-β codes for the flowering repressor FLM protein, translation of FLM-δ
    has the opposite effect on flowering. Here we show that the cyclin-dependent kinase
    G2 (CDKG2), together with its cognate cyclin, CYCLYN L1 (CYCL1) affects the alternative
    splicing of FLM, balancing the levels of FLM-β and FLM-δ across the ambient temperature
    range. In the absence of the CDKG2/CYCL1 complex, FLM-β expression is reduced
    while FLM-δ is increased in a temperature dependent manner and these changes are
    associated with an early flowering phenotype in the cdkg2 mutant lines. In addition,
    we found that transcript variants retaining the full FLM intron 1 are sequestered
    in the cell nucleus. Strikingly, FLM intron 1 splicing is also regulated by CDKG2/CYCL1.
    Our results provide evidence that temperature and CDKs regulate the alternative
    splicing of FLM, contributing to flowering time definition.
article_number: '1680'
article_processing_charge: No
article_type: original
author:
- first_name: Candida
  full_name: Nibau, Candida
  last_name: Nibau
- first_name: Marçal
  full_name: Gallemi, Marçal
  id: 460C6802-F248-11E8-B48F-1D18A9856A87
  last_name: Gallemi
  orcid: 0000-0003-4675-6893
- first_name: Despoina
  full_name: Dadarou, Despoina
  last_name: Dadarou
- first_name: John H.
  full_name: Doonan, John H.
  last_name: Doonan
- first_name: Nicola
  full_name: Cavallari, Nicola
  id: 457160E6-F248-11E8-B48F-1D18A9856A87
  last_name: Cavallari
citation:
  ama: Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. Thermo-sensitive alternative
    splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. <i>Frontiers
    in Plant Science</i>. 2020;10. doi:<a href="https://doi.org/10.3389/fpls.2019.01680">10.3389/fpls.2019.01680</a>
  apa: Nibau, C., Gallemi, M., Dadarou, D., Doonan, J. H., &#38; Cavallari, N. (2020).
    Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent
    kinase G2. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href="https://doi.org/10.3389/fpls.2019.01680">https://doi.org/10.3389/fpls.2019.01680</a>
  chicago: Nibau, Candida, Marçal Gallemi, Despoina Dadarou, John H. Doonan, and Nicola
    Cavallari. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated
    by Cyclin-Dependent Kinase G2.” <i>Frontiers in Plant Science</i>. Frontiers Media,
    2020. <a href="https://doi.org/10.3389/fpls.2019.01680">https://doi.org/10.3389/fpls.2019.01680</a>.
  ieee: C. Nibau, M. Gallemi, D. Dadarou, J. H. Doonan, and N. Cavallari, “Thermo-sensitive
    alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase
    G2,” <i>Frontiers in Plant Science</i>, vol. 10. Frontiers Media, 2020.
  ista: Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. 2020. Thermo-sensitive
    alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase
    G2. Frontiers in Plant Science. 10, 1680.
  mla: Nibau, Candida, et al. “Thermo-Sensitive Alternative Splicing of FLOWERING
    LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” <i>Frontiers in Plant Science</i>,
    vol. 10, 1680, Frontiers Media, 2020, doi:<a href="https://doi.org/10.3389/fpls.2019.01680">10.3389/fpls.2019.01680</a>.
  short: C. Nibau, M. Gallemi, D. Dadarou, J.H. Doonan, N. Cavallari, Frontiers in
    Plant Science 10 (2020).
corr_author: '1'
date_created: 2020-01-22T15:23:57Z
date_published: 2020-01-22T00:00:00Z
date_updated: 2025-06-12T07:33:02Z
day: '22'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.3389/fpls.2019.01680
external_id:
  isi:
  - '000511376000001'
  pmid:
  - '32038671'
file:
- access_level: open_access
  checksum: d1f92e60a713fbd15097ce895e5c7ccb
  content_type: application/pdf
  creator: dernst
  date_created: 2020-01-27T09:07:02Z
  date_updated: 2020-07-14T12:47:56Z
  file_id: '7366'
  file_name: 2020_FrontiersPlantScience_Nibau.pdf
  file_size: 1951438
  relation: main_file
file_date_updated: 2020-07-14T12:47:56Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Plant Science
publication_identifier:
  issn:
  - 1664-462X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by
  cyclin-dependent kinase G2
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 10
year: '2020'
...