---
_id: '15266'
abstract:
- lang: eng
text: Plant pathogens often exploit a whole range of effectors to facilitate infection.
The RXLR effector AVR1 produced by the oomycete plant pathogen Phytophthora infestans
suppresses host defense by targeting Sec5. Sec5 is a subunit of the exocyst, a
protein complex that is important for mediating polarized exocytosis during plant
development and defense against pathogens. The mechanism by which AVR1 manipulates
Sec5 functioning is unknown. In this study, we analyzed the effect of AVR1 on
Sec5 localization and functioning in the moss Physcomitrium patens. P. patens
has four Sec5 homologs. Two (PpSec5b and PpSec5d) were found to interact with
AVR1 in yeast-two-hybrid assays while none of the four showed a positive interaction
with AVR1ΔT, a truncated version of AVR1. In P. patens lines carrying β-estradiol
inducible AVR1 or AVR1ΔT transgenes, expression of AVR1 or AVR1ΔT caused defects
in the development of caulonemal protonema cells and abnormal morphology of chloronema
cells. Similar phenotypes were observed in Sec5- or Sec6-silenced P. patens lines,
suggesting that both AVR1 and AVR1ΔT affect exocyst functioning in P. patens.
With respect to Sec5 localization we found no differences between β-estradiol-treated
and untreated transgenic AVR1 lines. Sec5 localizes at the plasma membrane in
growing caulonema cells, also during pathogen attack, and its subcellular localization
is the same, with or without AVR1 in the vicinity.
article_number: e0249637
article_processing_charge: Yes
article_type: original
author:
- first_name: Elysa J. R.
full_name: Overdijk, Elysa J. R.
last_name: Overdijk
- first_name: Vera
full_name: Putker, Vera
last_name: Putker
- first_name: Joep
full_name: Smits, Joep
last_name: Smits
- first_name: Han
full_name: Tang, Han
id: 19BDF720-25A0-11EA-AC6E-928F3DDC885E
last_name: Tang
orcid: 0000-0001-6152-6637
- first_name: Klaas
full_name: Bouwmeester, Klaas
last_name: Bouwmeester
- first_name: Francine
full_name: Govers, Francine
last_name: Govers
- first_name: Tijs
full_name: Ketelaar, Tijs
last_name: Ketelaar
citation:
ama: Overdijk EJR, Putker V, Smits J, et al. Phytophthora infestans RXLR effector
AVR1 disturbs the growth of Physcomitrium patens without affecting Sec5 localization.
PLoS One. 2021;16(4). doi:10.1371/journal.pone.0249637
apa: Overdijk, E. J. R., Putker, V., Smits, J., Tang, H., Bouwmeester, K., Govers,
F., & Ketelaar, T. (2021). Phytophthora infestans RXLR effector AVR1 disturbs
the growth of Physcomitrium patens without affecting Sec5 localization. PLoS
One. Public Library of Science. https://doi.org/10.1371/journal.pone.0249637
chicago: Overdijk, Elysa J. R., Vera Putker, Joep Smits, Han Tang, Klaas Bouwmeester,
Francine Govers, and Tijs Ketelaar. “Phytophthora Infestans RXLR Effector AVR1
Disturbs the Growth of Physcomitrium Patens without Affecting Sec5 Localization.”
PLoS One. Public Library of Science, 2021. https://doi.org/10.1371/journal.pone.0249637.
ieee: E. J. R. Overdijk et al., “Phytophthora infestans RXLR effector AVR1
disturbs the growth of Physcomitrium patens without affecting Sec5 localization,”
PLoS One, vol. 16, no. 4. Public Library of Science, 2021.
ista: Overdijk EJR, Putker V, Smits J, Tang H, Bouwmeester K, Govers F, Ketelaar
T. 2021. Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium
patens without affecting Sec5 localization. PLoS One. 16(4), e0249637.
mla: Overdijk, Elysa J. R., et al. “Phytophthora Infestans RXLR Effector AVR1 Disturbs
the Growth of Physcomitrium Patens without Affecting Sec5 Localization.” PLoS
One, vol. 16, no. 4, e0249637, Public Library of Science, 2021, doi:10.1371/journal.pone.0249637.
short: E.J.R. Overdijk, V. Putker, J. Smits, H. Tang, K. Bouwmeester, F. Govers,
T. Ketelaar, PLoS One 16 (2021).
date_created: 2024-04-03T07:38:14Z
date_published: 2021-04-08T00:00:00Z
date_updated: 2024-04-29T06:53:15Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1371/journal.pone.0249637
external_id:
pmid:
- '33831039'
file:
- access_level: open_access
checksum: 25b7b329435af57db2c95571a8ef32fe
content_type: application/pdf
creator: dernst
date_created: 2024-04-29T06:51:59Z
date_updated: 2024-04-29T06:51:59Z
file_id: '15349'
file_name: 2021_PlosOne_Overdijk.pdf
file_size: 4738995
relation: main_file
success: 1
file_date_updated: 2024-04-29T06:51:59Z
has_accepted_license: '1'
intvolume: ' 16'
issue: '4'
keyword:
- Multidisciplinary
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS One
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium
patens without affecting Sec5 localization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2021'
...
---
_id: '7548'
abstract:
- lang: eng
text: Although the aggregation of the amyloid-β peptide (Aβ) into amyloid fibrils
is a well-established hallmark of Alzheimer’s disease, the complex mechanisms
linking this process to neurodegeneration are still incompletely understood. The
nematode worm C. elegans is a valuable model organism through which to study these
mechanisms because of its simple nervous system and its relatively short lifespan.
Standard Aβ-based C. elegans models of Alzheimer’s disease are designed to study
the toxic effects of the overexpression of Aβ in the muscle or nervous systems.
However, the wide variety of effects associated with the tissue-level overexpression
of Aβ makes it difficult to single out and study specific cellular mechanisms
related to the onset of Alzheimer’s disease. Here, to better understand how to
investigate the early events affecting neuronal signalling, we created a C. elegans
model expressing Aβ42, the 42-residue form of Aβ, from a single-copy gene insertion
in just one pair of glutamatergic sensory neurons, the BAG neurons. In behavioural
assays, we found that the Aβ42-expressing animals displayed a subtle modulation
of the response to CO2, compared to controls. Ca2+ imaging revealed that the BAG
neurons in young Aβ42-expressing nematodes were activated more strongly than in
control animals, and that neuronal activation remained intact until old age. Taken
together, our results suggest that Aβ42-expression in this very subtle model of
AD is sufficient to modulate the behavioural response but not strong enough to
generate significant neurotoxicity, suggesting that slightly more aggressive perturbations
will enable effectively studies of the links between the modulation of a physiological
response and its associated neurotoxicity.
article_number: e0217746
article_processing_charge: No
article_type: original
author:
- first_name: Tessa
full_name: Sinnige, Tessa
last_name: Sinnige
- first_name: Prashanth
full_name: Ciryam, Prashanth
last_name: Ciryam
- first_name: Samuel
full_name: Casford, Samuel
last_name: Casford
- first_name: Christopher M.
full_name: Dobson, Christopher M.
last_name: Dobson
- first_name: Mario
full_name: de Bono, Mario
id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
last_name: de Bono
orcid: 0000-0001-8347-0443
- first_name: Michele
full_name: Vendruscolo, Michele
last_name: Vendruscolo
citation:
ama: Sinnige T, Ciryam P, Casford S, Dobson CM, de Bono M, Vendruscolo M. Expression
of the amyloid-β peptide in a single pair of C. elegans sensory neurons modulates
the associated behavioural response. PLOS ONE. 2019;14(5). doi:10.1371/journal.pone.0217746
apa: Sinnige, T., Ciryam, P., Casford, S., Dobson, C. M., de Bono, M., & Vendruscolo,
M. (2019). Expression of the amyloid-β peptide in a single pair of C. elegans
sensory neurons modulates the associated behavioural response. PLOS ONE.
Public Library of Science. https://doi.org/10.1371/journal.pone.0217746
chicago: Sinnige, Tessa, Prashanth Ciryam, Samuel Casford, Christopher M. Dobson,
Mario de Bono, and Michele Vendruscolo. “Expression of the Amyloid-β Peptide in
a Single Pair of C. Elegans Sensory Neurons Modulates the Associated Behavioural
Response.” PLOS ONE. Public Library of Science, 2019. https://doi.org/10.1371/journal.pone.0217746.
ieee: T. Sinnige, P. Ciryam, S. Casford, C. M. Dobson, M. de Bono, and M. Vendruscolo,
“Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons
modulates the associated behavioural response,” PLOS ONE, vol. 14, no.
5. Public Library of Science, 2019.
ista: Sinnige T, Ciryam P, Casford S, Dobson CM, de Bono M, Vendruscolo M. 2019.
Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons
modulates the associated behavioural response. PLOS ONE. 14(5), e0217746.
mla: Sinnige, Tessa, et al. “Expression of the Amyloid-β Peptide in a Single Pair
of C. Elegans Sensory Neurons Modulates the Associated Behavioural Response.”
PLOS ONE, vol. 14, no. 5, e0217746, Public Library of Science, 2019, doi:10.1371/journal.pone.0217746.
short: T. Sinnige, P. Ciryam, S. Casford, C.M. Dobson, M. de Bono, M. Vendruscolo,
PLOS ONE 14 (2019).
date_created: 2020-02-28T10:45:13Z
date_published: 2019-05-31T00:00:00Z
date_updated: 2021-01-12T08:14:08Z
day: '31'
doi: 10.1371/journal.pone.0217746
extern: '1'
intvolume: ' 14'
issue: '5'
language:
- iso: eng
month: '05'
oa_version: Published Version
publication: PLOS ONE
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: Expression of the amyloid-β peptide in a single pair of C. elegans sensory
neurons modulates the associated behavioural response
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2019'
...
---
_id: '1029'
abstract:
- lang: eng
text: RNA Polymerase II pauses and backtracks during transcription, with many consequences
for gene expression and cellular physiology. Here, we show that the energy required
to melt double-stranded nucleic acids in the transcription bubble predicts pausing
in Saccharomyces cerevisiae far more accurately than nucleosome roadblocks do.
In addition, the same energy difference also determines when the RNA polymerase
backtracks instead of continuing to move forward. This data-driven model corroborates—in
a genome wide and quantitative manner—previous evidence that sequence-dependent
thermodynamic features of nucleic acids influence both transcriptional pausing
and backtracking.
article_number: e0174066
article_processing_charge: Yes
author:
- first_name: Martin
full_name: Lukacisin, Martin
id: 298FFE8C-F248-11E8-B48F-1D18A9856A87
last_name: Lukacisin
orcid: 0000-0001-6549-4177
- first_name: Matthieu
full_name: Landon, Matthieu
last_name: Landon
- first_name: Rishi
full_name: Jajoo, Rishi
last_name: Jajoo
citation:
ama: Lukacisin M, Landon M, Jajoo R. Sequence-specific thermodynamic properties
of nucleic acids influence both transcriptional pausing and backtracking in yeast.
PLoS One. 2017;12(3). doi:10.1371/journal.pone.0174066
apa: Lukacisin, M., Landon, M., & Jajoo, R. (2017). Sequence-specific thermodynamic
properties of nucleic acids influence both transcriptional pausing and backtracking
in yeast. PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0174066
chicago: Lukacisin, Martin, Matthieu Landon, and Rishi Jajoo. “Sequence-Specific
Thermodynamic Properties of Nucleic Acids Influence Both Transcriptional Pausing
and Backtracking in Yeast.” PLoS One. Public Library of Science, 2017.
https://doi.org/10.1371/journal.pone.0174066.
ieee: M. Lukacisin, M. Landon, and R. Jajoo, “Sequence-specific thermodynamic properties
of nucleic acids influence both transcriptional pausing and backtracking in yeast,”
PLoS One, vol. 12, no. 3. Public Library of Science, 2017.
ista: Lukacisin M, Landon M, Jajoo R. 2017. Sequence-specific thermodynamic properties
of nucleic acids influence both transcriptional pausing and backtracking in yeast.
PLoS One. 12(3), e0174066.
mla: Lukacisin, Martin, et al. “Sequence-Specific Thermodynamic Properties of Nucleic
Acids Influence Both Transcriptional Pausing and Backtracking in Yeast.” PLoS
One, vol. 12, no. 3, e0174066, Public Library of Science, 2017, doi:10.1371/journal.pone.0174066.
short: M. Lukacisin, M. Landon, R. Jajoo, PLoS One 12 (2017).
date_created: 2018-12-11T11:49:46Z
date_published: 2017-03-16T00:00:00Z
date_updated: 2026-04-29T22:30:10Z
day: '16'
ddc:
- '570'
department:
- _id: ToBo
doi: 10.1371/journal.pone.0174066
external_id:
isi:
- '000396318300121'
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:47Z
date_updated: 2018-12-12T10:09:47Z
file_id: '4772'
file_name: IST-2017-800-v1+1_journal.pone.0174066.pdf
file_size: 3429381
relation: main_file
file_date_updated: 2018-12-12T10:09:47Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
publication: PLoS One
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
publist_id: '6361'
pubrep_id: '800'
quality_controlled: '1'
related_material:
record:
- id: '5556'
relation: popular_science
status: public
- id: '6392'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Sequence-specific thermodynamic properties of nucleic acids influence both
transcriptional pausing and backtracking in yeast
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2017'
...
---
_id: '682'
abstract:
- lang: eng
text: Left-right asymmetry is a fundamental feature of higher-order brain structure;
however, the molecular basis of brain asymmetry remains unclear. We recently identified
structural and functional asymmetries in mouse hippocampal circuitry that result
from the asymmetrical distribution of two distinct populations of pyramidal cell
synapses that differ in the density of the NMDA receptor subunit GluRε2 (also
known as NR2B, GRIN2B or GluN2B). By examining the synaptic distribution of ε2
subunits, we previously found that β2-microglobulin-deficient mice, which lack
cell surface expression of the vast majority of major histocompatibility complex
class I (MHCI) proteins, do not exhibit circuit asymmetry. In the present study,
we conducted electrophysiological and anatomical analyses on the hippocampal circuitry
of mice with a knockout of the paired immunoglobulin-like receptor B (PirB), an
MHCI receptor. As in β2-microglobulin-deficient mice, the PirB-deficient hippocampus
lacked circuit asymmetries. This finding that MHCI loss-of-function mice and PirB
knockout mice have identical phenotypes suggests that MHCI signals that produce
hippocampal asymmetries are transduced through PirB. Our results provide evidence
for a critical role of the MHCI/PirB signaling system in the generation of asymmetries
in hippocampal circuitry.
article_number: e0179377
article_processing_charge: No
article_type: original
author:
- first_name: Hikari
full_name: Ukai, Hikari
last_name: Ukai
- first_name: Aiko
full_name: Kawahara, Aiko
last_name: Kawahara
- first_name: Keiko
full_name: Hirayama, Keiko
last_name: Hirayama
- first_name: Matthew J
full_name: Case, Matthew J
id: 44B7CA5A-F248-11E8-B48F-1D18A9856A87
last_name: Case
- first_name: Shotaro
full_name: Aino, Shotaro
last_name: Aino
- first_name: Masahiro
full_name: Miyabe, Masahiro
last_name: Miyabe
- first_name: Ken
full_name: Wakita, Ken
last_name: Wakita
- first_name: Ryohei
full_name: Oogi, Ryohei
last_name: Oogi
- first_name: Michiyo
full_name: Kasayuki, Michiyo
last_name: Kasayuki
- first_name: Shihomi
full_name: Kawashima, Shihomi
last_name: Kawashima
- first_name: Shunichi
full_name: Sugimoto, Shunichi
last_name: Sugimoto
- first_name: Kanako
full_name: Chikamatsu, Kanako
last_name: Chikamatsu
- first_name: Noritaka
full_name: Nitta, Noritaka
last_name: Nitta
- first_name: Tsuneyuki
full_name: Koga, Tsuneyuki
last_name: Koga
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Toshiyuki
full_name: Takai, Toshiyuki
last_name: Takai
- first_name: Isao
full_name: Ito, Isao
last_name: Ito
citation:
ama: Ukai H, Kawahara A, Hirayama K, et al. PirB regulates asymmetries in hippocampal
circuitry. PLoS One. 2017;12(6). doi:10.1371/journal.pone.0179377
apa: Ukai, H., Kawahara, A., Hirayama, K., Case, M. J., Aino, S., Miyabe, M., …
Ito, I. (2017). PirB regulates asymmetries in hippocampal circuitry. PLoS One.
Public Library of Science. https://doi.org/10.1371/journal.pone.0179377
chicago: Ukai, Hikari, Aiko Kawahara, Keiko Hirayama, Matthew J Case, Shotaro Aino,
Masahiro Miyabe, Ken Wakita, et al. “PirB Regulates Asymmetries in Hippocampal
Circuitry.” PLoS One. Public Library of Science, 2017. https://doi.org/10.1371/journal.pone.0179377.
ieee: H. Ukai et al., “PirB regulates asymmetries in hippocampal circuitry,”
PLoS One, vol. 12, no. 6. Public Library of Science, 2017.
ista: Ukai H, Kawahara A, Hirayama K, Case MJ, Aino S, Miyabe M, Wakita K, Oogi
R, Kasayuki M, Kawashima S, Sugimoto S, Chikamatsu K, Nitta N, Koga T, Shigemoto
R, Takai T, Ito I. 2017. PirB regulates asymmetries in hippocampal circuitry.
PLoS One. 12(6), e0179377.
mla: Ukai, Hikari, et al. “PirB Regulates Asymmetries in Hippocampal Circuitry.”
PLoS One, vol. 12, no. 6, e0179377, Public Library of Science, 2017, doi:10.1371/journal.pone.0179377.
short: H. Ukai, A. Kawahara, K. Hirayama, M.J. Case, S. Aino, M. Miyabe, K. Wakita,
R. Oogi, M. Kasayuki, S. Kawashima, S. Sugimoto, K. Chikamatsu, N. Nitta, T. Koga,
R. Shigemoto, T. Takai, I. Ito, PLoS One 12 (2017).
date_created: 2018-12-11T11:47:54Z
date_published: 2017-06-01T00:00:00Z
date_updated: 2026-04-29T22:30:53Z
day: '01'
ddc:
- '571'
department:
- _id: RySh
doi: 10.1371/journal.pone.0179377
external_id:
isi:
- '000402923200125'
file:
- access_level: open_access
checksum: 24dd19c46fb1c761b0bcbbcd1025a3a8
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:12:16Z
date_updated: 2020-07-14T12:47:40Z
file_id: '4934'
file_name: IST-2017-897-v1+1_journal.pone.0179377.pdf
file_size: 5798454
relation: main_file
file_date_updated: 2020-07-14T12:47:40Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: PLoS One
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
publist_id: '7034'
pubrep_id: '897'
quality_controlled: '1'
related_material:
record:
- id: '51'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: PirB regulates asymmetries in hippocampal circuitry
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 12
year: '2017'
...
---
_id: '6142'
abstract:
- lang: eng
text: Defining the mutational landscape when individuals of a species grow separately
and diverge over many generations can provide insights into trait evolution. A
specific example of this involves studying changes associated with domestication
where different lines of the same wild stock have been cultivated independently
in different standard environments. Whole genome sequence comparison of such lines
permits estimation of mutation rates, inference of genes' ancestral states and
ancestry of existing strains, and correction of sequencing errors in genome databases.
Here we study domestication of the C. elegans Bristol strain as a model, and report
the genome sequence of LSJ1 (Bristol), a sibling of the standard C. elegans reference
wild type N2 (Bristol). The LSJ1 and N2 lines were cultivated separately from
shortly after the Bristol strain was isolated until methods to freeze C. elegans
were developed. We find that during this time the two strains have accumulated
1208 genetic differences. We describe phenotypic variation between N2 and LSJ1
in the rate at which embryos develop, the rate of production of eggs, the maturity
of eggs at laying, and feeding behavior, all the result of post-isolation changes.
We infer the ancestral alleles in the original Bristol isolate and highlight 2038
likely sequencing errors in the original N2 reference genome sequence. Many of
these changes modify genome annotation. Our study provides a starting point to
further investigate genotype-phenotype association and offers insights into the
process of selection as a result of laboratory domestication.
article_number: e13922
author:
- first_name: Katherine P.
full_name: Weber, Katherine P.
last_name: Weber
- first_name: Subhajyoti
full_name: De, Subhajyoti
last_name: De
- first_name: Iwanka
full_name: Kozarewa, Iwanka
last_name: Kozarewa
- first_name: Daniel J.
full_name: Turner, Daniel J.
last_name: Turner
- first_name: M. Madan
full_name: Babu, M. Madan
last_name: Babu
- first_name: Mario
full_name: de Bono, Mario
id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
last_name: de Bono
orcid: 0000-0001-8347-0443
citation:
ama: Weber KP, De S, Kozarewa I, Turner DJ, Babu MM, de Bono M. Whole genome sequencing
highlights genetic changes associated with laboratory domestication of C. elegans.
PLoS ONE. 2010;5(11). doi:10.1371/journal.pone.0013922
apa: Weber, K. P., De, S., Kozarewa, I., Turner, D. J., Babu, M. M., & de Bono,
M. (2010). Whole genome sequencing highlights genetic changes associated with
laboratory domestication of C. elegans. PLoS ONE. Public Library of Science.
https://doi.org/10.1371/journal.pone.0013922
chicago: Weber, Katherine P., Subhajyoti De, Iwanka Kozarewa, Daniel J. Turner,
M. Madan Babu, and Mario de Bono. “Whole Genome Sequencing Highlights Genetic
Changes Associated with Laboratory Domestication of C. Elegans.” PLoS ONE.
Public Library of Science, 2010. https://doi.org/10.1371/journal.pone.0013922.
ieee: K. P. Weber, S. De, I. Kozarewa, D. J. Turner, M. M. Babu, and M. de Bono,
“Whole genome sequencing highlights genetic changes associated with laboratory
domestication of C. elegans,” PLoS ONE, vol. 5, no. 11. Public Library
of Science, 2010.
ista: Weber KP, De S, Kozarewa I, Turner DJ, Babu MM, de Bono M. 2010. Whole genome
sequencing highlights genetic changes associated with laboratory domestication
of C. elegans. PLoS ONE. 5(11), e13922.
mla: Weber, Katherine P., et al. “Whole Genome Sequencing Highlights Genetic Changes
Associated with Laboratory Domestication of C. Elegans.” PLoS ONE, vol.
5, no. 11, e13922, Public Library of Science, 2010, doi:10.1371/journal.pone.0013922.
short: K.P. Weber, S. De, I. Kozarewa, D.J. Turner, M.M. Babu, M. de Bono, PLoS
ONE 5 (2010).
date_created: 2019-03-20T15:20:30Z
date_published: 2010-11-11T00:00:00Z
date_updated: 2021-01-12T08:06:20Z
day: '11'
ddc:
- '570'
doi: 10.1371/journal.pone.0013922
extern: '1'
external_id:
pmid:
- '21085631'
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checksum: a01e6bbe15f044c0c79a26d7d881953e
content_type: application/pdf
creator: kschuh
date_created: 2019-03-20T15:22:48Z
date_updated: 2020-07-14T12:47:20Z
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file_name: 2010_PLOS_Weber.PDF
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has_accepted_license: '1'
intvolume: ' 5'
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS ONE
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: Whole genome sequencing highlights genetic changes associated with laboratory
domestication of C. elegans
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2010'
...
---
_id: '11114'
abstract:
- lang: eng
text: We present a miniaturized pull-down method for the detection of protein-protein
interactions using standard affinity chromatography reagents. Binding events between
different proteins, which are color-coded with quantum dots (QDs), are visualized
on single affinity chromatography beads by fluorescence microscopy. The use of
QDs for single molecule detection allows the simultaneous analysis of multiple
protein-protein binding events and reduces the amount of time and material needed
to perform a pull-down experiment.
article_number: e2061
article_processing_charge: No
article_type: original
author:
- first_name: Roberta
full_name: Schulte, Roberta
last_name: Schulte
- first_name: Jessica
full_name: Talamas, Jessica
last_name: Talamas
- first_name: Christine
full_name: Doucet, Christine
last_name: Doucet
- first_name: Martin W
full_name: HETZER, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: HETZER
orcid: 0000-0002-2111-992X
citation:
ama: Schulte R, Talamas J, Doucet C, Hetzer M. Single bead affinity detection (SINBAD)
for the analysis of protein-protein interactions. PLoS ONE. 2008;3(4).
doi:10.1371/journal.pone.0002061
apa: Schulte, R., Talamas, J., Doucet, C., & Hetzer, M. (2008). Single bead
affinity detection (SINBAD) for the analysis of protein-protein interactions.
PLoS ONE. Public Library of Science. https://doi.org/10.1371/journal.pone.0002061
chicago: Schulte, Roberta, Jessica Talamas, Christine Doucet, and Martin Hetzer.
“Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions.”
PLoS ONE. Public Library of Science, 2008. https://doi.org/10.1371/journal.pone.0002061.
ieee: R. Schulte, J. Talamas, C. Doucet, and M. Hetzer, “Single bead affinity detection
(SINBAD) for the analysis of protein-protein interactions,” PLoS ONE, vol.
3, no. 4. Public Library of Science, 2008.
ista: Schulte R, Talamas J, Doucet C, Hetzer M. 2008. Single bead affinity detection
(SINBAD) for the analysis of protein-protein interactions. PLoS ONE. 3(4), e2061.
mla: Schulte, Roberta, et al. “Single Bead Affinity Detection (SINBAD) for the Analysis
of Protein-Protein Interactions.” PLoS ONE, vol. 3, no. 4, e2061, Public
Library of Science, 2008, doi:10.1371/journal.pone.0002061.
short: R. Schulte, J. Talamas, C. Doucet, M. Hetzer, PLoS ONE 3 (2008).
date_created: 2022-04-07T07:55:57Z
date_published: 2008-04-30T00:00:00Z
date_updated: 2024-10-14T11:29:56Z
day: '30'
doi: 10.1371/journal.pone.0002061
extern: '1'
external_id:
pmid:
- '18446240'
intvolume: ' 3'
issue: '4'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: ' https://doi.org/10.1371/journal.pone.0002061'
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS ONE
publication_identifier:
issn:
- 1932-6203
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Single bead affinity detection (SINBAD) for the analysis of protein-protein
interactions
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2008'
...