[{"project":[{"_id":"9B8F7476-BA93-11EA-9121-9846C619BF3A","name":"HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery"}],"external_id":{"pmid":["38829127"],"isi":["001281657200005"]},"date_created":"2024-06-09T22:01:02Z","title":"Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity","author":[{"first_name":"Christine","last_name":"Fiedler","id":"bd3fceba-dc74-11ea-a0a7-c17f71817366","full_name":"Fiedler, Christine"},{"full_name":"Liu, Yu","id":"2A70014E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7313-6740","last_name":"Liu","first_name":"Yu"},{"full_name":"Ibáñez, Maria","id":"43C61214-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5013-2843","last_name":"Ibáñez","first_name":"Maria"}],"date_updated":"2025-09-08T07:51:46Z","abstract":[{"text":"In recent years, solution processes have gained considerable traction as a cost-effective and scalable method to produce high-performance thermoelectric materials. The process entails a series of critical steps: synthesis, purification, thermal treatments, and consolidation, each playing a pivotal role in determining performance, stability, and reproducibility. We have noticed a need for more comprehensive details for each of the described steps in most published works. Recognizing the significance of detailed synthetic protocols, we describe here the approach used to synthesize and characterize one of the highest-performing polycrystalline p-type SnSe. In particular, we report the synthesis of SnSe particles in water and the subsequent surface treatment with CdSe molecular complexes that yields CdSe-SnSe nanocomposites upon consolidation. Moreover, the surface treatment inhibits grain growth through Zenner pinning of secondary phase CdSe nanoparticles and enhances defect formation at different length scales. The enhanced complexity in the CdSe-SnSe nanocomposite microstructure with respect to SnSe promotes phonon scattering and thereby significantly reduces the thermal conductivity. Such surface engineering provides opportunities in solution processing for introducing and controlling defects, making it possible to optimize the transport properties and attain a high thermoelectric figure of merit.","lang":"eng"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","pmid":1,"publication_status":"published","publication":"Journal of Visualized Experiments","file_date_updated":"2025-02-17T15:08:55Z","OA_type":"hybrid","date_published":"2024-05-01T00:00:00Z","article_number":"e66278","corr_author":"1","quality_controlled":"1","citation":{"short":"C. Fiedler, Y. Liu, M. Ibáñez, Journal of Visualized Experiments 2024 (2024).","mla":"Fiedler, Christine, et al. “Solution-Processed, Surface-Engineered, Polycrystalline CdSe-SnSe Exhibiting Low Thermal Conductivity.” <i>Journal of Visualized Experiments</i>, vol. 2024, no. 207, e66278, MyJove Corporation, 2024, doi:<a href=\"https://doi.org/10.3791/66278\">10.3791/66278</a>.","ieee":"C. Fiedler, Y. Liu, and M. Ibáñez, “Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity,” <i>Journal of Visualized Experiments</i>, vol. 2024, no. 207. MyJove Corporation, 2024.","ista":"Fiedler C, Liu Y, Ibáñez M. 2024. Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity. Journal of Visualized Experiments. 2024(207), e66278.","chicago":"Fiedler, Christine, Yu Liu, and Maria Ibáñez. “Solution-Processed, Surface-Engineered, Polycrystalline CdSe-SnSe Exhibiting Low Thermal Conductivity.” <i>Journal of Visualized Experiments</i>. MyJove Corporation, 2024. <a href=\"https://doi.org/10.3791/66278\">https://doi.org/10.3791/66278</a>.","apa":"Fiedler, C., Liu, Y., &#38; Ibáñez, M. (2024). Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity. <i>Journal of Visualized Experiments</i>. MyJove Corporation. <a href=\"https://doi.org/10.3791/66278\">https://doi.org/10.3791/66278</a>","ama":"Fiedler C, Liu Y, Ibáñez M. Solution-processed, surface-engineered, polycrystalline CdSe-SnSe exhibiting low thermal conductivity. <i>Journal of Visualized Experiments</i>. 2024;2024(207). doi:<a href=\"https://doi.org/10.3791/66278\">10.3791/66278</a>"},"ddc":["530"],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"}],"publisher":"MyJove Corporation","scopus_import":"1","article_type":"original","intvolume":"      2024","department":[{"_id":"MaIb"}],"day":"01","_id":"17124","oa_version":"Published Version","article_processing_charge":"Yes (in subscription journal)","month":"05","license":"https://creativecommons.org/licenses/by-nc-nd/3.0/","isi":1,"year":"2024","oa":1,"language":[{"iso":"eng"}],"file":[{"relation":"main_file","date_created":"2025-02-17T15:08:55Z","content_type":"application/pdf","file_size":1371995,"checksum":"ddb41f1ce2333484ab5cd109ac2941c0","date_updated":"2025-02-17T15:08:55Z","file_name":"2024_JoVE_Fiedler.pdf","file_id":"19047","success":1,"creator":"dernst","access_level":"open_access"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/3.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)","short":"CC BY-NC-ND (3.0)","image":"/images/cc_by_nc_nd.png"},"doi":"10.3791/66278","issue":"207","has_accepted_license":"1","volume":2024,"OA_place":"publisher","publication_identifier":{"issn":["1940-087X"]},"status":"public","APC_amount":"4394,84 EUR","acknowledgement":"The Scientific Service Units (SSU) of ISTA supported this research through resources provided by the Electron Microscopy Facility (EMF) and the Lab Support Facility (LSF). This work was financially supported by the Institute of Science and Technology Austria and the Werner Siemens Foundation.","type":"journal_article"},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"publisher":"MyJove Corporation","quality_controlled":"1","citation":{"ieee":"R. J. Beattie <i>et al.</i>, “Lineage tracing and clonal analysis in developing cerebral cortex using mosaic analysis with double markers (MADM),” <i>Journal of Visual Experiments</i>, no. 159. MyJove Corporation, 2020.","short":"R.J. Beattie, C. Streicher, N. Amberg, G.T. Cheung, X. Contreras, A.H. Hansen, S. Hippenmeyer, Journal of Visual Experiments (2020).","mla":"Beattie, Robert J., et al. “Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM).” <i>Journal of Visual Experiments</i>, no. 159, e61147, MyJove Corporation, 2020, doi:<a href=\"https://doi.org/10.3791/61147\">10.3791/61147</a>.","ista":"Beattie RJ, Streicher C, Amberg N, Cheung GT, Contreras X, Hansen AH, Hippenmeyer S. 2020. Lineage tracing and clonal analysis in developing cerebral cortex using mosaic analysis with double markers (MADM). Journal of Visual Experiments. (159), e61147.","chicago":"Beattie, Robert J, Carmen Streicher, Nicole Amberg, Giselle T Cheung, Ximena Contreras, Andi H Hansen, and Simon Hippenmeyer. “Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM).” <i>Journal of Visual Experiments</i>. MyJove Corporation, 2020. <a href=\"https://doi.org/10.3791/61147\">https://doi.org/10.3791/61147</a>.","apa":"Beattie, R. J., Streicher, C., Amberg, N., Cheung, G. T., Contreras, X., Hansen, A. H., &#38; Hippenmeyer, S. (2020). Lineage tracing and clonal analysis in developing cerebral cortex using mosaic analysis with double markers (MADM). <i>Journal of Visual Experiments</i>. MyJove Corporation. <a href=\"https://doi.org/10.3791/61147\">https://doi.org/10.3791/61147</a>","ama":"Beattie RJ, Streicher C, Amberg N, et al. Lineage tracing and clonal analysis in developing cerebral cortex using mosaic analysis with double markers (MADM). <i>Journal of Visual Experiments</i>. 2020;(159). doi:<a href=\"https://doi.org/10.3791/61147\">10.3791/61147</a>"},"ddc":["570"],"day":"08","_id":"7815","article_processing_charge":"No","oa_version":"Published Version","article_type":"original","scopus_import":"1","department":[{"_id":"SiHi"}],"date_updated":"2026-06-15T22:31:06Z","abstract":[{"lang":"eng","text":"Beginning from a limited pool of progenitors, the mammalian cerebral cortex forms highly organized functional neural circuits. However, the underlying cellular and molecular mechanisms regulating lineage transitions of neural stem cells (NSCs) and eventual production of neurons and glia in the developing neuroepithelium remains unclear. Methods to trace NSC division patterns and map the lineage of clonally related cells have advanced dramatically. However, many contemporary lineage tracing techniques suffer from the lack of cellular resolution of progeny cell fate, which is essential for deciphering progenitor cell division patterns. Presented is a protocol using mosaic analysis with double markers (MADM) to perform in vivo clonal analysis. MADM concomitantly manipulates individual progenitor cells and visualizes precise division patterns and lineage progression at unprecedented single cell resolution. MADM-based interchromosomal recombination events during the G2-X phase of mitosis, together with temporally inducible CreERT2, provide exact information on the birth dates of clones and their division patterns. Thus, MADM lineage tracing provides unprecedented qualitative and quantitative optical readouts of the proliferation mode of stem cell progenitors at the single cell level. MADM also allows for examination of the mechanisms and functional requirements of candidate genes in NSC lineage progression. This method is unique in that comparative analysis of control and mutant subclones can be performed in the same tissue environment in vivo. Here, the protocol is described in detail, and experimental paradigms to employ MADM for clonal analysis and lineage tracing in the developing cerebral cortex are demonstrated. Importantly, this protocol can be adapted to perform MADM clonal analysis in any murine stem cell niche, as long as the CreERT2 driver is present."}],"project":[{"name":"Molecular Mechanisms Regulating Gliogenesis in the Neocortex","grant_number":"M02416","call_identifier":"FWF","_id":"264E56E2-B435-11E9-9278-68D0E5697425"},{"_id":"268F8446-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"T01031","name":"Role of Eed in neural stem cell lineage progression"},{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"grant_number":"24812","name":"Molecular mechanisms of radial neuronal migration","_id":"2625A13E-B435-11E9-9278-68D0E5697425"},{"call_identifier":"H2020","grant_number":"725780","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","_id":"260018B0-B435-11E9-9278-68D0E5697425"}],"date_created":"2020-05-11T08:31:20Z","external_id":{"pmid":["32449730"],"isi":["000546406600043"]},"title":"Lineage tracing and clonal analysis in developing cerebral cortex using mosaic analysis with double markers (MADM)","author":[{"orcid":"0000-0002-8483-8753","first_name":"Robert J","last_name":"Beattie","id":"2E26DF60-F248-11E8-B48F-1D18A9856A87","full_name":"Beattie, Robert J"},{"last_name":"Streicher","first_name":"Carmen","full_name":"Streicher, Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Nicole","last_name":"Amberg","orcid":"0000-0002-3183-8207","full_name":"Amberg, Nicole","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Cheung","first_name":"Giselle T","orcid":"0000-0001-8457-2572","id":"471195F6-F248-11E8-B48F-1D18A9856A87","full_name":"Cheung, Giselle T"},{"last_name":"Contreras","first_name":"Ximena","full_name":"Contreras, Ximena","id":"475990FE-F248-11E8-B48F-1D18A9856A87"},{"id":"38853E16-F248-11E8-B48F-1D18A9856A87","full_name":"Hansen, Andi H","first_name":"Andi H","last_name":"Hansen"},{"id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061","last_name":"Hippenmeyer","first_name":"Simon"}],"date_published":"2020-05-08T00:00:00Z","article_number":"e61147","corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","pmid":1,"file_date_updated":"2020-07-14T12:48:03Z","publication":"Journal of Visual Experiments","publication_identifier":{"issn":["1940-087X"]},"status":"public","ec_funded":1,"type":"journal_article","isi":1,"month":"05","doi":"10.3791/61147","issue":"159","has_accepted_license":"1","related_material":{"record":[{"status":"public","id":"7902","relation":"part_of_dissertation"}]},"year":"2020","oa":1,"file":[{"relation":"main_file","date_created":"2020-05-11T08:28:38Z","file_size":1352186,"content_type":"application/pdf","checksum":"3154ea7f90b9fb45e084cd1c2770597d","file_name":"jove-protocol-61147-lineage-tracing-clonal-analysis-developing-cerebral-cortex-using.pdf","date_updated":"2020-07-14T12:48:03Z","file_id":"7816","creator":"rbeattie","access_level":"open_access"}],"language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}}]