---
_id: '601'
abstract:
- lang: eng
  text: 'The conserved polymerase-Associated factor 1 complex (Paf1C) plays multiple
    roles in chromatin transcription and genomic regulation. Paf1C comprises the five
    subunits Paf1, Leo1, Ctr9, Cdc73 and Rtf1, and binds to the RNA polymerase II
    (Pol II) transcription elongation complex (EC). Here we report the reconstitution
    of Paf1C from Saccharomyces cerevisiae, and a structural analysis of Paf1C bound
    to a Pol II EC containing the elongation factor TFIIS. Cryo-electron microscopy
    and crosslinking data reveal that Paf1C is highly mobile and extends over the
    outer Pol II surface from the Rpb2 to the Rpb3 subunit. The Paf1-Leo1 heterodimer
    and Cdc73 form opposite ends of Paf1C, whereas Ctr9 bridges between them. Consistent
    with the structural observations, the initiation factor TFIIF impairs Paf1C binding
    to Pol II, whereas the elongation factor TFIIS enhances it. We further show that
    Paf1C is globally required for normal mRNA transcription in yeast. These results
    provide a three-dimensional framework for further analysis of Paf1C function in
    transcription through chromatin. '
article_number: '15741'
article_processing_charge: No
author:
- first_name: Youwei
  full_name: Xu, Youwei
  last_name: Xu
- first_name: Carrie A
  full_name: Bernecky, Carrie A
  id: 2CB9DFE2-F248-11E8-B48F-1D18A9856A87
  last_name: Bernecky
  orcid: 0000-0003-0893-7036
- first_name: Chung
  full_name: Lee, Chung
  last_name: Lee
- first_name: Kerstin
  full_name: Maier, Kerstin
  last_name: Maier
- first_name: Björn
  full_name: Schwalb, Björn
  last_name: Schwalb
- first_name: Dimitri
  full_name: Tegunov, Dimitri
  last_name: Tegunov
- first_name: Jürgen
  full_name: Plitzko, Jürgen
  last_name: Plitzko
- first_name: Henning
  full_name: Urlaub, Henning
  last_name: Urlaub
- first_name: Patrick
  full_name: Cramer, Patrick
  last_name: Cramer
citation:
  ama: Xu Y, Bernecky C, Lee C, et al. Architecture of the RNA polymerase II-Paf1C-TFIIS
    transcription elongation complex. <i>Nature Communications</i>. 2017;8. doi:<a
    href="https://doi.org/10.1038/ncomms15741">10.1038/ncomms15741</a>
  apa: Xu, Y., Bernecky, C., Lee, C., Maier, K., Schwalb, B., Tegunov, D., … Cramer,
    P. (2017). Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation
    complex. <i>Nature Communications</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/ncomms15741">https://doi.org/10.1038/ncomms15741</a>
  chicago: Xu, Youwei, Carrie Bernecky, Chung Lee, Kerstin Maier, Björn Schwalb, Dimitri
    Tegunov, Jürgen Plitzko, Henning Urlaub, and Patrick Cramer. “Architecture of
    the RNA Polymerase II-Paf1C-TFIIS Transcription Elongation Complex.” <i>Nature
    Communications</i>. Nature Publishing Group, 2017. <a href="https://doi.org/10.1038/ncomms15741">https://doi.org/10.1038/ncomms15741</a>.
  ieee: Y. Xu <i>et al.</i>, “Architecture of the RNA polymerase II-Paf1C-TFIIS transcription
    elongation complex,” <i>Nature Communications</i>, vol. 8. Nature Publishing Group,
    2017.
  ista: Xu Y, Bernecky C, Lee C, Maier K, Schwalb B, Tegunov D, Plitzko J, Urlaub
    H, Cramer P. 2017. Architecture of the RNA polymerase II-Paf1C-TFIIS transcription
    elongation complex. Nature Communications. 8, 15741.
  mla: Xu, Youwei, et al. “Architecture of the RNA Polymerase II-Paf1C-TFIIS Transcription
    Elongation Complex.” <i>Nature Communications</i>, vol. 8, 15741, Nature Publishing
    Group, 2017, doi:<a href="https://doi.org/10.1038/ncomms15741">10.1038/ncomms15741</a>.
  short: Y. Xu, C. Bernecky, C. Lee, K. Maier, B. Schwalb, D. Tegunov, J. Plitzko,
    H. Urlaub, P. Cramer, Nature Communications 8 (2017).
date_created: 2018-12-11T11:47:25Z
date_published: 2017-06-06T00:00:00Z
date_updated: 2021-01-12T08:05:40Z
day: '06'
ddc:
- '570'
doi: 10.1038/ncomms15741
extern: '1'
file:
- access_level: open_access
  checksum: 940742282a9a285dc4aeae0c2b5ebe96
  content_type: application/pdf
  creator: dernst
  date_created: 2019-01-21T14:48:10Z
  date_updated: 2020-07-14T12:47:16Z
  file_id: '5865'
  file_name: 2017_NatureComm_Xu.pdf
  file_size: 3018075
  relation: main_file
file_date_updated: 2020-07-14T12:47:16Z
fulldoi: https://doi.org/10.1038/ncomms15741
has_accepted_license: '1'
intvolume: '         8'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_identifier:
  issn:
  - '20411723'
publication_status: published
publisher: Nature Publishing Group
publist_id: '7203'
quality_controlled: '1'
status: public
title: Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation
  complex
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2017'
...
---
_id: '835'
abstract:
- lang: eng
  text: An outstanding question in animal development, tissue homeostasis and disease
    is how cell populations adapt to sensory inputs. During Drosophila larval development,
    hematopoietic sites are in direct contact with sensory neuron clusters of the
    peripheral nervous system (PNS), and blood cells (hemocytes) require the PNS for
    their survival and recruitment to these microenvironments, known as Hematopoietic
    Pockets. Here we report that Activin-β, a TGF-β family ligand, is expressed by
    sensory neurons of the PNS and regulates the proliferation and adhesion of hemocytes.
    These hemocyte responses depend on PNS activity, as shown by agonist treatment
    and transient silencing of sensory neurons. Activin-β has a key role in this regulation,
    which is apparent from reporter expression and mutant analyses. This mechanism
    of local sensory neurons controlling blood cell adaptation invites evolutionary
    parallels with vertebrate hematopoietic progenitors and the independent myeloid
    system of tissue macrophages, whose regulation by local microenvironments remain
    undefined.
article_number: '15990'
article_processing_charge: No
author:
- first_name: Kalpana
  full_name: Makhijani, Kalpana
  last_name: Makhijani
- first_name: Brandy
  full_name: Alexander, Brandy
  last_name: Alexander
- first_name: Deepti
  full_name: Rao, Deepti
  last_name: Rao
- first_name: Sophia
  full_name: Petraki, Sophia
  last_name: Petraki
- first_name: Leire
  full_name: Herboso, Leire
  last_name: Herboso
- first_name: Katelyn
  full_name: Kukar, Katelyn
  last_name: Kukar
- first_name: Itrat
  full_name: Batool, Itrat
  last_name: Batool
- first_name: Stephanie
  full_name: Wachner, Stephanie
  id: 2A95E7B0-F248-11E8-B48F-1D18A9856A87
  last_name: Wachner
- first_name: Katrina
  full_name: Gold, Katrina
  last_name: Gold
- first_name: Corinna
  full_name: Wong, Corinna
  last_name: Wong
- first_name: Michael
  full_name: O'Connor, Michael
  last_name: O'Connor
- first_name: Katja
  full_name: Brückner, Katja
  last_name: Brückner
citation:
  ama: Makhijani K, Alexander B, Rao D, et al. Regulation of Drosophila hematopoietic
    sites by Activin-β from active sensory neurons. <i>Nature Communications</i>.
    2017;8. doi:<a href="https://doi.org/10.1038/ncomms15990">10.1038/ncomms15990</a>
  apa: Makhijani, K., Alexander, B., Rao, D., Petraki, S., Herboso, L., Kukar, K.,
    … Brückner, K. (2017). Regulation of Drosophila hematopoietic sites by Activin-β
    from active sensory neurons. <i>Nature Communications</i>. Nature Publishing Group.
    <a href="https://doi.org/10.1038/ncomms15990">https://doi.org/10.1038/ncomms15990</a>
  chicago: Makhijani, Kalpana, Brandy Alexander, Deepti Rao, Sophia Petraki, Leire
    Herboso, Katelyn Kukar, Itrat Batool, et al. “Regulation of Drosophila Hematopoietic
    Sites by Activin-β from Active Sensory Neurons.” <i>Nature Communications</i>.
    Nature Publishing Group, 2017. <a href="https://doi.org/10.1038/ncomms15990">https://doi.org/10.1038/ncomms15990</a>.
  ieee: K. Makhijani <i>et al.</i>, “Regulation of Drosophila hematopoietic sites
    by Activin-β from active sensory neurons,” <i>Nature Communications</i>, vol.
    8. Nature Publishing Group, 2017.
  ista: Makhijani K, Alexander B, Rao D, Petraki S, Herboso L, Kukar K, Batool I,
    Wachner S, Gold K, Wong C, O’Connor M, Brückner K. 2017. Regulation of Drosophila
    hematopoietic sites by Activin-β from active sensory neurons. Nature Communications.
    8, 15990.
  mla: Makhijani, Kalpana, et al. “Regulation of Drosophila Hematopoietic Sites by
    Activin-β from Active Sensory Neurons.” <i>Nature Communications</i>, vol. 8,
    15990, Nature Publishing Group, 2017, doi:<a href="https://doi.org/10.1038/ncomms15990">10.1038/ncomms15990</a>.
  short: K. Makhijani, B. Alexander, D. Rao, S. Petraki, L. Herboso, K. Kukar, I.
    Batool, S. Wachner, K. Gold, C. Wong, M. O’Connor, K. Brückner, Nature Communications
    8 (2017).
date_created: 2018-12-11T11:48:45Z
date_published: 2017-07-27T00:00:00Z
date_updated: 2023-09-26T15:51:28Z
day: '27'
ddc:
- '570'
- '576'
- '616'
doi: 10.1038/ncomms15990
extern: '1'
external_id:
  isi:
  - '000406360100001'
file:
- access_level: open_access
  checksum: 99a3d63308d4250eda0a35341171f80e
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:15:32Z
  date_updated: 2020-07-14T12:48:12Z
  file_id: '5153'
  file_name: IST-2017-859-v1+1_ncomms15990.pdf
  file_size: 3027104
  relation: main_file
file_date_updated: 2020-07-14T12:48:12Z
fulldoi: https://doi.org/10.1038/ncomms15990
has_accepted_license: '1'
intvolume: '         8'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_identifier:
  issn:
  - '20411723'
publication_status: published
publisher: Nature Publishing Group
publist_id: '6813'
pubrep_id: '859'
quality_controlled: '1'
status: public
title: Regulation of Drosophila hematopoietic sites by Activin-β from active sensory
  neurons
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2017'
...