---
OA_place: repository
OA_type: green
_id: '19422'
abstract:
- lang: eng
text: Nitrogen (N) is an essential macronutrient for plant development and, ultimately,
yield. Identifying the genetic components and mechanisms underlying N use efficiency
in maize (Zea mays L.) is thus of great importance. Nitrate (NO3−) is the preferred
inorganic N source in maize. Here we performed a genome-wide association study
of shoot NO3− accumulation in maize seedlings grown under low-NO3− conditions,
identifying the ferredoxin family gene ZmFd4 as a major contributor to this trait.
ZmFd4 interacts and co-localizes with nitrite reductases (ZmNiRs) in chloroplasts
to promote their enzymatic activity. Furthermore, ZmFd4 forms a high-affinity
heterodimer with its closest paralogue, ZmFd9, in a NO3−-sensitive manner. Although
ZmFd4 exerts similar biochemical functions as ZmFd9, ZmFd4 and ZmFd9 interaction
limits their ability to associate with ZmNiRs and stimulate their activity. Knockout
lines for ZmFd4 with decreased NO3− contents exhibit more efficient NO3− assimilation,
and field experiments show consistently improved N utilization and grain yield
under N-deficient conditions. Our work thus provides molecular and mechanistic
insights into the natural variation in N utilization, instrumental for genetic
improvement of yield in maize and, potentially, in other crops.
acknowledgement: We thank X. Yang for providing published inbred lines and helping
with data analysis; and S. Huang, C. Jiang, G. Bi, C. Liu and S. Zhang for helpful
discussions. The transgenic maize lines were generated by the Center for Crop Functional
Genomics and Molecular Breeding of China Agricultural University. This work was
supported by grants from the National Key Research and Development Program of China
(2021YFF1000500 to J.Z.), the National Natural Science Foundation of China (32170265
and 32441022 to J.Z.), the Chinese Universities Scientific Fund (2024TC084 to J.Z.),
the Pinduoduo-China Agricultural University Research Fund (PC2024B01005 to J.Z.),
the Hainan Provincial Natural Science Foundation of China (323CXTD379 to J.Z.),
and the Central Guidance on Local Science and Technology Development Fund of Shanxi
Province (YDZJSX2024D040 to C.T. and J.Z.).
article_number: '5207'
article_processing_charge: No
article_type: original
author:
- first_name: G
full_name: Jia, G
last_name: Jia
- first_name: G
full_name: Chen, G
last_name: Chen
- first_name: Z
full_name: Zhang, Z
last_name: Zhang
- first_name: C
full_name: Tian, C
last_name: Tian
- first_name: Y
full_name: Wang, Y
last_name: Wang
- first_name: J
full_name: Luo, J
last_name: Luo
- first_name: K
full_name: Zhang, K
last_name: Zhang
- first_name: X
full_name: Zhao, X
last_name: Zhao
- first_name: X
full_name: Zhao, X
last_name: Zhao
- first_name: Z
full_name: Li, Z
last_name: Li
- first_name: L
full_name: Sun, L
last_name: Sun
- first_name: W
full_name: Yang, W
last_name: Yang
- first_name: Y
full_name: Guo, Y
last_name: Guo
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Z
full_name: Gong, Z
last_name: Gong
- first_name: J
full_name: Zhang, J
last_name: Zhang
citation:
ama: Jia G, Chen G, Zhang Z, et al. Ferredoxin-mediated mechanism for efficient
nitrogen utilization in maize. Nature Plants. 2025;11. doi:10.1038/s41477-025-01934-w
apa: Jia, G., Chen, G., Zhang, Z., Tian, C., Wang, Y., Luo, J., … Zhang, J. (2025).
Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize. Nature
Plants. Springer Nature. https://doi.org/10.1038/s41477-025-01934-w
chicago: Jia, G, G Chen, Z Zhang, C Tian, Y Wang, J Luo, K Zhang, et al. “Ferredoxin-Mediated
Mechanism for Efficient Nitrogen Utilization in Maize.” Nature Plants.
Springer Nature, 2025. https://doi.org/10.1038/s41477-025-01934-w.
ieee: G. Jia et al., “Ferredoxin-mediated mechanism for efficient nitrogen
utilization in maize,” Nature Plants, vol. 11. Springer Nature, 2025.
ista: Jia G, Chen G, Zhang Z, Tian C, Wang Y, Luo J, Zhang K, Zhao X, Zhao X, Li
Z, Sun L, Yang W, Guo Y, Friml J, Gong Z, Zhang J. 2025. Ferredoxin-mediated mechanism
for efficient nitrogen utilization in maize. Nature Plants. 11, 5207.
mla: Jia, G., et al. “Ferredoxin-Mediated Mechanism for Efficient Nitrogen Utilization
in Maize.” Nature Plants, vol. 11, 5207, Springer Nature, 2025, doi:10.1038/s41477-025-01934-w.
short: G. Jia, G. Chen, Z. Zhang, C. Tian, Y. Wang, J. Luo, K. Zhang, X. Zhao, X.
Zhao, Z. Li, L. Sun, W. Yang, Y. Guo, J. Friml, Z. Gong, J. Zhang, Nature Plants
11 (2025).
date_created: 2025-03-19T09:44:55Z
date_published: 2025-03-05T00:00:00Z
date_updated: 2025-11-12T07:52:06Z
day: '05'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-025-01934-w
external_id:
isi:
- '001437953800001'
pmid:
- '40044942'
file:
- access_level: open_access
checksum: caeaf1a8bc3e1435e8c995d1d9df5390
content_type: application/pdf
creator: dernst
date_created: 2025-11-12T07:50:45Z
date_updated: 2025-11-12T07:50:45Z
file_id: '20634'
file_name: 2025_NaturePlants_Jia_submitted.pdf
file_size: 2714177
relation: main_file
success: 1
file_date_updated: 2025-11-12T07:50:45Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20479'
abstract:
- lang: eng
text: 'Genetic variation is generally regarded as a prerequisite for evolution.
In principle, epigenetic information inherited independently of DNA sequence can
also enable evolution, but whether this occurs in natural populations is unknown.
Here we show that single-nucleotide and epigenetic gene body DNA methylation (gbM)
polymorphisms explain comparable amounts of expression variance in Arabidopsis
thaliana populations. We genetically demonstrate that gbM regulates
transcription, and we identify and genetically validate many associations between
gbM polymorphism and the variation of complex traits: fitness under heat and drought,
flowering time and accumulation of diverse minerals. Epigenome-wide association
studies pinpoint trait-relevant genes with greater precision than genetic association
analyses, probably due to reduced linkage disequilibrium between gbM variants.
Finally, we identify numerous associations between gbM epialleles and diverse
environmental conditions in native habitats, suggesting that gbM facilitates adaptation.
Overall, our results indicate that epigenetic methylation variation fundamentally
shapes phenotypic diversity in a natural population.'
acknowledgement: We thank P. Baduel and V. Colot for sharing SV data, A. Muyle for
gbM conservation data and X. Feng, C. Dean, E. Coen and Zilberman lab members for
constructive comments on the paper. This work was supported by a European Research
Council grant (725746) to D.Z., LUMS Startup grant (STG-188) to Z.S. and US National
Science Foundation grant (MCB-2334561) to H.R. This study would not have been possible
without Arabidopsis 1001 genome, methylome and transcriptome resources. Open access
funding provided by Institute of Science and Technology (IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Zaigham
full_name: Shahzad, Zaigham
last_name: Shahzad
- first_name: Elizabeth
full_name: Hollwey, Elizabeth
id: b8c4f54b-e484-11eb-8fdc-a54df64ef6dd
last_name: Hollwey
- first_name: Jonathan D.
full_name: Moore, Jonathan D.
last_name: Moore
- first_name: Jaemyung
full_name: Choi, Jaemyung
last_name: Choi
- first_name: Gaëlle
full_name: Cassin-Ross, Gaëlle
last_name: Cassin-Ross
- first_name: Hatem
full_name: Rouached, Hatem
last_name: Rouached
- first_name: Matthew Richard
full_name: Robinson, Matthew Richard
id: E5D42276-F5DA-11E9-8E24-6303E6697425
last_name: Robinson
orcid: 0000-0001-8982-8813
- first_name: Daniel
full_name: Zilberman, Daniel
id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1
last_name: Zilberman
orcid: 0000-0002-0123-8649
citation:
ama: Shahzad Z, Hollwey E, Moore JD, et al. Gene body methylation regulates gene
expression and mediates phenotypic diversity in natural Arabidopsis populations.
Nature Plants. 2025;11:2084-2099. doi:10.1038/s41477-025-02108-4
apa: Shahzad, Z., Hollwey, E., Moore, J. D., Choi, J., Cassin-Ross, G., Rouached,
H., … Zilberman, D. (2025). Gene body methylation regulates gene expression and
mediates phenotypic diversity in natural Arabidopsis populations. Nature Plants.
Springer Nature. https://doi.org/10.1038/s41477-025-02108-4
chicago: Shahzad, Zaigham, Elizabeth Hollwey, Jonathan D. Moore, Jaemyung Choi,
Gaëlle Cassin-Ross, Hatem Rouached, Matthew Richard Robinson, and Daniel Zilberman.
“Gene Body Methylation Regulates Gene Expression and Mediates Phenotypic Diversity
in Natural Arabidopsis Populations.” Nature Plants. Springer Nature, 2025.
https://doi.org/10.1038/s41477-025-02108-4.
ieee: Z. Shahzad et al., “Gene body methylation regulates gene expression
and mediates phenotypic diversity in natural Arabidopsis populations,” Nature
Plants, vol. 11. Springer Nature, pp. 2084–2099, 2025.
ista: Shahzad Z, Hollwey E, Moore JD, Choi J, Cassin-Ross G, Rouached H, Robinson
MR, Zilberman D. 2025. Gene body methylation regulates gene expression and mediates
phenotypic diversity in natural Arabidopsis populations. Nature Plants. 11, 2084–2099.
mla: Shahzad, Zaigham, et al. “Gene Body Methylation Regulates Gene Expression and
Mediates Phenotypic Diversity in Natural Arabidopsis Populations.” Nature Plants,
vol. 11, Springer Nature, 2025, pp. 2084–99, doi:10.1038/s41477-025-02108-4.
short: Z. Shahzad, E. Hollwey, J.D. Moore, J. Choi, G. Cassin-Ross, H. Rouached,
M.R. Robinson, D. Zilberman, Nature Plants 11 (2025) 2084–2099.
corr_author: '1'
date_created: 2025-10-16T13:11:21Z
date_published: 2025-09-12T00:00:00Z
date_updated: 2025-12-01T14:59:10Z
day: '12'
ddc:
- '580'
department:
- _id: MaRo
- _id: DaZi
doi: 10.1038/s41477-025-02108-4
ec_funded: 1
external_id:
isi:
- '001570197600001'
pmid:
- '40940427'
file:
- access_level: open_access
checksum: 6a3f6cffdc934b8a2015c3c247f5a92a
content_type: application/pdf
creator: dernst
date_created: 2025-10-23T11:13:58Z
date_updated: 2025-10-23T11:13:58Z
file_id: '20524'
file_name: 2025_NaturePlants_Shahzad.pdf
file_size: 7746662
relation: main_file
success: 1
file_date_updated: 2025-10-23T11:13:58Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 2084-2099
pmid: 1
project:
- _id: 62935a00-2b32-11ec-9570-eff30fa39068
call_identifier: H2020
grant_number: '725746'
name: Quantitative analysis of DNA methylation maintenance with chromatin
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Gene body methylation regulates gene expression and mediates phenotypic diversity
in natural Arabidopsis populations
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2025'
...
---
_id: '14339'
abstract:
- lang: eng
text: Lateral roots are typically maintained at non-vertical angles with respect
to gravity. These gravitropic setpoint angles are intriguing because their maintenance
requires that roots are able to effect growth response both with and against the
gravity vector, a phenomenon previously attributed to gravitropism acting against
an antigravitropic offset mechanism. Here we show how the components mediating
gravitropism in the vertical primary root—PINs and phosphatases acting upon them—are
reconfigured in their regulation such that lateral root growth at a range of angles
can be maintained. We show that the ability of Arabidopsis lateral roots to bend
both downward and upward requires the generation of auxin asymmetries and is driven
by angle-dependent variation in downward gravitropic auxin flux acting against
angle-independent upward, antigravitropic flux. Further, we demonstrate a symmetry
in auxin distribution in lateral roots at gravitropic setpoint angle that can
be traced back to a net, balanced polarization of PIN3 and PIN7 auxin transporters
in the columella. These auxin fluxes are shifted by altering PIN protein phosphoregulation
in the columella, either by introducing PIN3 phosphovariant versions or via manipulation
of levels of the phosphatase subunit PP2A/RCN1. Finally, we show that auxin, in
addition to driving lateral root directional growth, acts within the lateral root
columella to induce more vertical growth by increasing RCN1 levels, causing a
downward shift in PIN3 localization, thereby diminishing the magnitude of the
upward, antigravitropic auxin flux.
acknowledgement: We thank D. Weijers, C. Schwechheimer and R. Offringa for generous
sharing of published and unpublished materials and P. Masson for advice on the use
of the ARL2 promoter. We are grateful to M. Del Bianco and O. Leyser for critical
reading of the manuscript. This work was supported by the BBSRC (grants BB/N010124/1
and BB/R000859/1 to S.K.), the Gatsby Charitable Foundation and the Leverhulme Trust
(RPG-2018-137 to S.K.).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: S
full_name: Roychoudhry, S
last_name: Roychoudhry
- first_name: K
full_name: Sageman-Furnas, K
last_name: Sageman-Furnas
- first_name: C
full_name: Wolverton, C
last_name: Wolverton
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: M
full_name: De Angelis, M
last_name: De Angelis
- first_name: HL
full_name: Goodman, HL
last_name: Goodman
- first_name: N
full_name: Capstaff, N
last_name: Capstaff
- first_name: Lloyd
full_name: JPB, Lloyd
last_name: JPB
- first_name: J
full_name: Mullen, J
last_name: Mullen
- first_name: R
full_name: Hangarter, R
last_name: Hangarter
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: S
full_name: Kepinski, S
last_name: Kepinski
citation:
ama: Roychoudhry S, Sageman-Furnas K, Wolverton C, et al. Antigravitropic PIN polarization
maintains non-vertical growth in lateral roots. Nature Plants. 2023;9:1500-1513.
doi:10.1038/s41477-023-01478-x
apa: Roychoudhry, S., Sageman-Furnas, K., Wolverton, C., Grones, P., Tan, S., Molnar,
G., … Kepinski, S. (2023). Antigravitropic PIN polarization maintains non-vertical
growth in lateral roots. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-023-01478-x
chicago: Roychoudhry, S, K Sageman-Furnas, C Wolverton, Peter Grones, Shutang Tan,
Gergely Molnar, M De Angelis, et al. “Antigravitropic PIN Polarization Maintains
Non-Vertical Growth in Lateral Roots.” Nature Plants. Springer Nature,
2023. https://doi.org/10.1038/s41477-023-01478-x.
ieee: S. Roychoudhry et al., “Antigravitropic PIN polarization maintains
non-vertical growth in lateral roots,” Nature Plants, vol. 9. Springer
Nature, pp. 1500–1513, 2023.
ista: Roychoudhry S, Sageman-Furnas K, Wolverton C, Grones P, Tan S, Molnar G, De
Angelis M, Goodman H, Capstaff N, JPB L, Mullen J, Hangarter R, Friml J, Kepinski
S. 2023. Antigravitropic PIN polarization maintains non-vertical growth in lateral
roots. Nature Plants. 9, 1500–1513.
mla: Roychoudhry, S., et al. “Antigravitropic PIN Polarization Maintains Non-Vertical
Growth in Lateral Roots.” Nature Plants, vol. 9, Springer Nature, 2023,
pp. 1500–13, doi:10.1038/s41477-023-01478-x.
short: S. Roychoudhry, K. Sageman-Furnas, C. Wolverton, P. Grones, S. Tan, G. Molnar,
M. De Angelis, H. Goodman, N. Capstaff, L. JPB, J. Mullen, R. Hangarter, J. Friml,
S. Kepinski, Nature Plants 9 (2023) 1500–1513.
date_created: 2023-09-15T09:56:01Z
date_published: 2023-09-01T00:00:00Z
date_updated: 2024-10-21T06:01:33Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-023-01478-x
external_id:
isi:
- '001069238800014'
pmid:
- '37666965'
file:
- access_level: open_access
checksum: 3d6d5d5abb937c14a5f6f0afba3b8624
content_type: application/pdf
creator: dernst
date_created: 2023-09-20T10:51:31Z
date_updated: 2023-09-20T10:51:31Z
file_id: '14351'
file_name: 2023_NaturePlants_Roychoudhry.pdf
file_size: 9647103
relation: main_file
success: 1
file_date_updated: 2023-09-20T10:51:31Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 1500-1513
pmid: 1
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Antigravitropic PIN polarization maintains non-vertical growth in lateral roots
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2023'
...
---
_id: '7106'
abstract:
- lang: eng
text: PIN-FORMED (PIN) transporters mediate directional, intercellular movement
of the phytohormone auxin in land plants. To elucidate the evolutionary origins
of this developmentally crucial mechanism, we analysed the single PIN homologue
of a simple green alga Klebsormidium flaccidum. KfPIN functions as a plasma membrane-localized
auxin exporter in land plants and heterologous models. While its role in algae
remains unclear, PIN-driven auxin export is probably an ancient and conserved
trait within streptophytes.
article_processing_charge: No
article_type: original
author:
- first_name: Roman
full_name: Skokan, Roman
last_name: Skokan
- first_name: Eva
full_name: Medvecká, Eva
last_name: Medvecká
- first_name: Tom
full_name: Viaene, Tom
last_name: Viaene
- first_name: Stanislav
full_name: Vosolsobě, Stanislav
last_name: Vosolsobě
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Karel
full_name: Müller, Karel
last_name: Müller
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Michal
full_name: Karady, Michal
last_name: Karady
- first_name: Yuzhou
full_name: Zhang, Yuzhou
last_name: Zhang
- first_name: Dorina P.
full_name: Janacek, Dorina P.
last_name: Janacek
- first_name: Ulrich Z.
full_name: Hammes, Ulrich Z.
last_name: Hammes
- first_name: Karin
full_name: Ljung, Karin
last_name: Ljung
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Skokan R, Medvecká E, Viaene T, et al. PIN-driven auxin transport emerged early
in streptophyte evolution. Nature Plants. 2019;5(11):1114-1119. doi:10.1038/s41477-019-0542-5
apa: Skokan, R., Medvecká, E., Viaene, T., Vosolsobě, S., Zwiewka, M., Müller, K.,
… Friml, J. (2019). PIN-driven auxin transport emerged early in streptophyte evolution.
Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-019-0542-5
chicago: Skokan, Roman, Eva Medvecká, Tom Viaene, Stanislav Vosolsobě, Marta Zwiewka,
Karel Müller, Petr Skůpa, et al. “PIN-Driven Auxin Transport Emerged Early in
Streptophyte Evolution.” Nature Plants. Springer Nature, 2019. https://doi.org/10.1038/s41477-019-0542-5.
ieee: R. Skokan et al., “PIN-driven auxin transport emerged early in streptophyte
evolution,” Nature Plants, vol. 5, no. 11. Springer Nature, pp. 1114–1119,
2019.
ista: Skokan R, Medvecká E, Viaene T, Vosolsobě S, Zwiewka M, Müller K, Skůpa P,
Karady M, Zhang Y, Janacek DP, Hammes UZ, Ljung K, Nodzyński T, Petrášek J, Friml
J. 2019. PIN-driven auxin transport emerged early in streptophyte evolution. Nature
Plants. 5(11), 1114–1119.
mla: Skokan, Roman, et al. “PIN-Driven Auxin Transport Emerged Early in Streptophyte
Evolution.” Nature Plants, vol. 5, no. 11, Springer Nature, 2019, pp. 1114–19,
doi:10.1038/s41477-019-0542-5.
short: R. Skokan, E. Medvecká, T. Viaene, S. Vosolsobě, M. Zwiewka, K. Müller, P.
Skůpa, M. Karady, Y. Zhang, D.P. Janacek, U.Z. Hammes, K. Ljung, T. Nodzyński,
J. Petrášek, J. Friml, Nature Plants 5 (2019) 1114–1119.
date_created: 2019-11-25T09:08:04Z
date_published: 2019-11-01T00:00:00Z
date_updated: 2025-04-14T07:45:04Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-019-0542-5
ec_funded: 1
external_id:
isi:
- '000496526100010'
pmid:
- '31712756'
file:
- access_level: open_access
checksum: 94e0426856aad9a9bd0135d5436efbf1
content_type: application/pdf
creator: dernst
date_created: 2020-10-14T08:54:49Z
date_updated: 2020-10-14T08:54:49Z
file_id: '8660'
file_name: 2019_NaturePlants_Skokan_accepted.pdf
file_size: 1980851
relation: main_file
success: 1
file_date_updated: 2020-10-14T08:54:49Z
has_accepted_license: '1'
intvolume: ' 5'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: 1114-1119
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: PIN-driven auxin transport emerged early in streptophyte evolution
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 5
year: '2019'
...
---
_id: '5673'
abstract:
- lang: eng
text: Cell polarity, manifested by the localization of proteins to distinct polar
plasma membrane domains, is a key prerequisite of multicellular life. In plants,
PIN auxin transporters are prominent polarity markers crucial for a plethora of
developmental processes. Cell polarity mechanisms in plants are distinct from
other eukaryotes and still largely elusive. In particular, how the cell polarities
are propagated and maintained following cell division remains unknown. Plant cytokinesis
is orchestrated by the cell plate—a transient centrifugally growing endomembrane
compartment ultimately forming the cross wall1. Trafficking of polar membrane
proteins is typically redirected to the cell plate, and these will consequently
have opposite polarity in at least one of the daughter cells2–5. Here, we provide
mechanistic insights into post-cytokinetic re-establishment of cell polarity as
manifested by the apical, polar localization of PIN2. We show that the apical
domain is defined in a cell-intrinsic manner and that re-establishment of PIN2
localization to this domain requires de novo protein secretion and endocytosis,
but not basal-to-apical transcytosis. Furthermore, we identify a PINOID-related
kinase WAG1, which phosphorylates PIN2 in vitro6 and is transcriptionally upregulated
specifically in dividing cells, as a crucial regulator of post-cytokinetic PIN2
polarity re-establishment.
article_processing_charge: No
author:
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Glanc M, Fendrych M, Friml J. Mechanistic framework for cell-intrinsic re-establishment
of PIN2 polarity after cell division. Nature Plants. 2018;4(12):1082-1088.
doi:10.1038/s41477-018-0318-3
apa: Glanc, M., Fendrych, M., & Friml, J. (2018). Mechanistic framework for
cell-intrinsic re-establishment of PIN2 polarity after cell division. Nature
Plants. Nature Research. https://doi.org/10.1038/s41477-018-0318-3
chicago: Glanc, Matous, Matyas Fendrych, and Jiří Friml. “Mechanistic Framework
for Cell-Intrinsic Re-Establishment of PIN2 Polarity after Cell Division.” Nature
Plants. Nature Research, 2018. https://doi.org/10.1038/s41477-018-0318-3.
ieee: M. Glanc, M. Fendrych, and J. Friml, “Mechanistic framework for cell-intrinsic
re-establishment of PIN2 polarity after cell division,” Nature Plants,
vol. 4, no. 12. Nature Research, pp. 1082–1088, 2018.
ista: Glanc M, Fendrych M, Friml J. 2018. Mechanistic framework for cell-intrinsic
re-establishment of PIN2 polarity after cell division. Nature Plants. 4(12), 1082–1088.
mla: Glanc, Matous, et al. “Mechanistic Framework for Cell-Intrinsic Re-Establishment
of PIN2 Polarity after Cell Division.” Nature Plants, vol. 4, no. 12, Nature
Research, 2018, pp. 1082–88, doi:10.1038/s41477-018-0318-3.
short: M. Glanc, M. Fendrych, J. Friml, Nature Plants 4 (2018) 1082–1088.
date_created: 2018-12-16T22:59:18Z
date_published: 2018-12-03T00:00:00Z
date_updated: 2025-04-14T07:45:02Z
day: '03'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0318-3
ec_funded: 1
external_id:
isi:
- '000454576600017'
pmid:
- '30518833'
intvolume: ' 4'
isi: 1
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30518833
month: '12'
oa: 1
oa_version: Submitted Version
page: 1082-1088
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Nature Research
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanistic framework for cell-intrinsic re-establishment of PIN2 polarity
after cell division
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2018'
...