---
_id: '11056'
abstract:
- lang: eng
  text: Aging of the circulatory system correlates with the pathogenesis of a large
    spectrum of diseases. However, it is largely unknown which factors drive the age-dependent
    or pathological decline of the vasculature and how vascular defects relate to
    tissue aging. The goal of the study is to design a multianalytical approach to
    identify how the cellular microenvironment (i.e., fibroblasts) and serum from
    healthy donors of different ages or Alzheimer disease (AD) patients can modulate
    the functionality of organ-specific vascular endothelial cells (VECs). Long-living
    human microvascular networks embedding VECs and fibroblasts from skin biopsies
    are generated. RNA-seq, secretome analyses, and microfluidic assays demonstrate
    that fibroblasts from young donors restore the functionality of aged endothelial
    cells, an effect also achieved by serum from young donors. New biomarkers of vascular
    aging are validated in human biopsies and it is shown that young serum induces
    angiopoietin-like-4, which can restore compromised vascular barriers. This strategy
    is then employed to characterize transcriptional/functional changes induced on
    the blood–brain barrier by AD serum, demonstrating the importance of PTP4A3 in
    the regulation of permeability. Features of vascular degeneration during aging
    and AD are recapitulated, and a tool to identify novel biomarkers that can be
    exploited to develop future therapeutics modulating vascular function is established.
article_number: '2000044'
article_processing_charge: No
article_type: original
author:
- first_name: Simone
  full_name: Bersini, Simone
  last_name: Bersini
- first_name: Rafael
  full_name: Arrojo e Drigo, Rafael
  last_name: Arrojo e Drigo
- first_name: Ling
  full_name: Huang, Ling
  last_name: Huang
- first_name: Maxim N.
  full_name: Shokhirev, Maxim N.
  last_name: Shokhirev
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: Bersini S, Arrojo e Drigo R, Huang L, Shokhirev MN, Hetzer M. Transcriptional
    and functional changes of the human microvasculature during physiological aging
    and Alzheimer disease. <i>Advanced Biosystems</i>. 2020;4(5). doi:<a href="https://doi.org/10.1002/adbi.202000044">10.1002/adbi.202000044</a>
  apa: Bersini, S., Arrojo e Drigo, R., Huang, L., Shokhirev, M. N., &#38; Hetzer,
    M. (2020). Transcriptional and functional changes of the human microvasculature
    during physiological aging and Alzheimer disease. <i>Advanced Biosystems</i>.
    Wiley. <a href="https://doi.org/10.1002/adbi.202000044">https://doi.org/10.1002/adbi.202000044</a>
  chicago: Bersini, Simone, Rafael Arrojo e Drigo, Ling Huang, Maxim N. Shokhirev,
    and Martin Hetzer. “Transcriptional and Functional Changes of the Human Microvasculature
    during Physiological Aging and Alzheimer Disease.” <i>Advanced Biosystems</i>.
    Wiley, 2020. <a href="https://doi.org/10.1002/adbi.202000044">https://doi.org/10.1002/adbi.202000044</a>.
  ieee: S. Bersini, R. Arrojo e Drigo, L. Huang, M. N. Shokhirev, and M. Hetzer, “Transcriptional
    and functional changes of the human microvasculature during physiological aging
    and Alzheimer disease,” <i>Advanced Biosystems</i>, vol. 4, no. 5. Wiley, 2020.
  ista: Bersini S, Arrojo e Drigo R, Huang L, Shokhirev MN, Hetzer M. 2020. Transcriptional
    and functional changes of the human microvasculature during physiological aging
    and Alzheimer disease. Advanced Biosystems. 4(5), 2000044.
  mla: Bersini, Simone, et al. “Transcriptional and Functional Changes of the Human
    Microvasculature during Physiological Aging and Alzheimer Disease.” <i>Advanced
    Biosystems</i>, vol. 4, no. 5, 2000044, Wiley, 2020, doi:<a href="https://doi.org/10.1002/adbi.202000044">10.1002/adbi.202000044</a>.
  short: S. Bersini, R. Arrojo e Drigo, L. Huang, M.N. Shokhirev, M. Hetzer, Advanced
    Biosystems 4 (2020).
date_created: 2022-04-07T07:43:57Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2024-10-14T11:18:07Z
day: '01'
ddc:
- '570'
doi: 10.1002/adbi.202000044
extern: '1'
external_id:
  pmid:
  - '32402127'
file:
- access_level: open_access
  checksum: 5584d9a1609812dc75c02ce1e35d2ec0
  content_type: application/pdf
  creator: dernst
  date_created: 2022-04-08T07:06:05Z
  date_updated: 2022-04-08T07:06:05Z
  file_id: '11134'
  file_name: 2020_AdvancedBiosystems_Bersini.pdf
  file_size: 2490829
  relation: main_file
  success: 1
file_date_updated: 2022-04-08T07:06:05Z
has_accepted_license: '1'
intvolume: '         4'
issue: '5'
keyword:
- General Biochemistry
- Genetics and Molecular Biology
- Biomedical Engineering
- Biomaterials
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
publication: Advanced Biosystems
publication_identifier:
  issn:
  - 2366-7478
  - 2366-7478
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Transcriptional and functional changes of the human microvasculature during
  physiological aging and Alzheimer disease
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2020'
...