[{"oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","status":"public","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"doi":"10.1016/j.celrep.2025.116904","quality_controlled":"1","article_processing_charge":"Yes","type":"journal_article","ddc":["570"],"_id":"21744","title":"A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types","OA_place":"publisher","file":[{"success":1,"file_id":"21793","checksum":"82098dd9d0ca609119f9f2c6beb4fc1e","file_name":"2026_CellReports_Li.pdf","date_updated":"2026-05-04T11:58:51Z","relation":"main_file","date_created":"2026-05-04T11:58:51Z","access_level":"open_access","creator":"dernst","file_size":38532865,"content_type":"application/pdf"}],"department":[{"_id":"AmDo"}],"publisher":"Elsevier","publication":"Cell Reports","has_accepted_license":"1","citation":{"ama":"Li Y, Butler TC, Nardone S, et al. A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. <i>Cell Reports</i>. 2026;45(2). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">10.1016/j.celrep.2025.116904</a>","mla":"Li, Yuxi, et al. “A Spatial and Projection-Based Transcriptomic Atlas of Paraventricular Hypothalamic Cell Types.” <i>Cell Reports</i>, vol. 45, no. 2, 116904, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">10.1016/j.celrep.2025.116904</a>.","apa":"Li, Y., Butler, T. C., Nardone, S., Jacobs, C. L., Douglass, A. M., Madara, J. C., … Resch, J. M. (2026). A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">https://doi.org/10.1016/j.celrep.2025.116904</a>","ieee":"Y. Li <i>et al.</i>, “A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types,” <i>Cell Reports</i>, vol. 45, no. 2. Elsevier, 2026.","short":"Y. Li, T.C. Butler, S. Nardone, C.L. Jacobs, A.M. Douglass, J.C. Madara, M.C. McDonough, J. Tao, E.D. Lowenstein, L. Wang, D. Pant, S.J. Walker, A. Wang, H. Srinivasan, Z. Yang, J.N. Campbell, L.T. Tsai, B.B. Lowell, J.M. Resch, Cell Reports 45 (2026).","ista":"Li Y, Butler TC, Nardone S, Jacobs CL, Douglass AM, Madara JC, McDonough MC, Tao J, Lowenstein ED, Wang L, Pant D, Walker SJ, Wang A, Srinivasan H, Yang Z, Campbell JN, Tsai LT, Lowell BB, Resch JM. 2026. A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types. Cell Reports. 45(2), 116904.","chicago":"Li, Yuxi, Trevor C. Butler, Stefano Nardone, Christopher L. Jacobs, Amelia M. Douglass, Joseph C. Madara, Miriam C. McDonough, et al. “A Spatial and Projection-Based Transcriptomic Atlas of Paraventricular Hypothalamic Cell Types.” <i>Cell Reports</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2025.116904\">https://doi.org/10.1016/j.celrep.2025.116904</a>."},"day":"24","OA_type":"gold","date_updated":"2026-05-04T12:00:31Z","DOAJ_listed":"1","publication_status":"published","tmp":{"image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"article_number":"116904","pmid":1,"external_id":{"pmid":["41581146"]},"author":[{"full_name":"Li, Yuxi","first_name":"Yuxi","last_name":"Li"},{"full_name":"Butler, Trevor C.","first_name":"Trevor C.","last_name":"Butler"},{"full_name":"Nardone, Stefano","first_name":"Stefano","last_name":"Nardone"},{"last_name":"Jacobs","first_name":"Christopher L.","full_name":"Jacobs, Christopher L."},{"last_name":"Douglass","first_name":"Amelia May Barnett","id":"de5f6fda-80fb-11ef-996f-a8c4ecd8e289","orcid":"0000-0001-5398-6473","full_name":"Douglass, Amelia May Barnett"},{"full_name":"Madara, Joseph C.","first_name":"Joseph C.","last_name":"Madara"},{"full_name":"McDonough, Miriam C.","first_name":"Miriam C.","last_name":"McDonough"},{"last_name":"Tao","first_name":"Jenkang","full_name":"Tao, Jenkang"},{"first_name":"Elijah D.","last_name":"Lowenstein","full_name":"Lowenstein, Elijah D."},{"first_name":"Luhong","last_name":"Wang","full_name":"Wang, Luhong"},{"last_name":"Pant","first_name":"Deepti","full_name":"Pant, Deepti"},{"full_name":"Walker, Samuel J.","first_name":"Samuel J.","last_name":"Walker"},{"first_name":"Annette","last_name":"Wang","full_name":"Wang, Annette"},{"full_name":"Srinivasan, Harini","last_name":"Srinivasan","first_name":"Harini"},{"first_name":"Zongfang","last_name":"Yang","full_name":"Yang, Zongfang"},{"full_name":"Campbell, John N.","first_name":"John N.","last_name":"Campbell"},{"last_name":"Tsai","first_name":"Linus T.","full_name":"Tsai, Linus T."},{"full_name":"Lowell, Bradford B.","first_name":"Bradford B.","last_name":"Lowell"},{"full_name":"Resch, Jon M.","last_name":"Resch","first_name":"Jon M."}],"date_created":"2026-04-16T13:51:29Z","file_date_updated":"2026-05-04T11:58:51Z","date_published":"2026-02-24T00:00:00Z","year":"2026","article_type":"original","intvolume":"        45","language":[{"iso":"eng"}],"acknowledgement":"We would like to thank Drs. Mark Andermann, Joel Geerling, and Clifford\r\nSaper, as well as the Lowell, Tsai, and Resch laboratories for helpful discussions;\r\nAlysia Berns, Jia Yu, and Yanfang Li for technical support; the BNORC\r\nFunctional Genomics and Bioinformatics Core (P30DK046200) and the Iowa\r\nInstitute for Human Genetics Genomics Division (IIHG, RRID: SCR_023422)\r\nfor helpful discussions and technical assistance with sc/snRNA-seq; Zachary\r\nNiziolek and the Bauer Core Facility at Harvard University, the BIDMC Flow Cytometry\r\nCore, and Heath Vignes, Michael Shey, and Thomas Kaufman of the\r\nFlow Cytometry Facility at the University of Iowa Carver College of Medicine\r\nfor helpful discussions and technical support; the ICCB-Longwood Screening\r\nFacility of Harvard Medical School for assistance with the snRNA-seq\r\nexperiments; Dr. Sayak Mitter and Vizgen support for technical assistance\r\nwith the MERSCOPE platform; and Mara Jendro and Li-Chun (Queena) Lin\r\nfor their assistance with MERSCOPE experiments within the Iowa\r\nNeuroBank Core in the Iowa Neuroscience Institute at the University of Iowa\r\nCarver College of Medicine. This research was funded by the following NIH\r\ngrants to L.T.T.: R01DK128406; to B.B.L.: R01DK075632, R01DK134427,\r\nand R01DK096010; to J.M.R.: R00HL144923 and R01NS141072; and to M.C.M.: F31HL170784; T.C.B. and M.C.M. were supported by a pharmacological\r\nsciences predoctoral training grant T32GM144636. Additional funding\r\nto J.M.R. came from the American Heart Association (AHA 935362), a University\r\nof Iowa Fraternal Order of Eagles Diabetes Research Center Pilot and\r\nFeasibility Catalyst Grant, and an Iowa Neuroscience Institute Early Stage\r\nInvestigator award from the Carver Trust. Y.L. was supported by a predoctoral\r\nfellowship from the American Heart Association (AHA 25PRE1372983). A.M.D.\r\nwas supported by a postdoctoral fellowship from the Charles A. King Trust.","oa":1,"issue":"2","month":"02","abstract":[{"text":"The paraventricular hypothalamus (PVH) controls behavioral and physiologic processes, including appetite, social behavior, autonomic outflow, and pituitary hormone secretion. However, molecular markers for centrally projecting PVH neuron populations remain largely undefined, and a complete census of PVH cell types has not been established. Therefore, we performed extensive single-cell/nucleus RNA sequencing to catalog PVH neuron subtypes and multiplexed error-robust fluorescence in situ hybridization (MERFISH) to map them spatially. Our spatial transcriptomic atlas resolves 26 Sim1+ and 29 GABAergic neuron populations from the PVH and surrounding areas. Additionally, projection-based profiling identified neurons that project to the parabrachial region (PB) and spinal cord, helping to determine PVH populations that regulate satiety and sympathetic nervous system activity, respectively. Notably, activation of PB-projecting PVH neurons expressing Brs3 reduces food intake, and silencing them causes obesity. Together, this atlas contributes high-resolution PVH spatial and circuit-based gene expression profiles, representing a valuable resource for the field of homeostasis.","lang":"eng"}],"volume":45},{"day":"28","date_updated":"2026-05-04T12:27:06Z","OA_type":"gold","OA_place":"publisher","_id":"21746","title":"Multifold increase in spinal inhibitory cell types with emergence of limb movement","citation":{"ama":"Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. 2026;45(4). doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>","ieee":"D. Vijatovic <i>et al.</i>, “Multifold increase in spinal inhibitory cell types with emergence of limb movement,” <i>Cell Reports</i>, vol. 45, no. 4. Elsevier, 2026.","mla":"Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>, vol. 45, no. 4, 117227, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">10.1016/j.celrep.2026.117227</a>.","apa":"Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M., Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory cell types with emergence of limb movement. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>","short":"D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild, M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton, M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).","chicago":"Vijatovic, David, Florina Alexandra  Toma, Y Ignatyev, Zoe P Harrington, Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell Reports</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.celrep.2026.117227\">https://doi.org/10.1016/j.celrep.2026.117227</a>.","ista":"Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory cell types with emergence of limb movement. Cell Reports. 45(4), 117227."},"publication":"Cell Reports","has_accepted_license":"1","publisher":"Elsevier","department":[{"_id":"LoSw"},{"_id":"GradSch"},{"_id":"TiVo"},{"_id":"Bio"},{"_id":"NiBa"}],"file":[{"creator":"dernst","content_type":"application/pdf","file_size":14925958,"relation":"main_file","date_created":"2026-05-04T12:20:10Z","access_level":"open_access","file_name":"2026_CellReports_Vijatovic.pdf","date_updated":"2026-05-04T12:20:10Z","success":1,"file_id":"21795","checksum":"0d26cdb5b8d8dec3a911d8261a65cdef"}],"type":"journal_article","article_processing_charge":"Yes","ddc":["570"],"oa_version":"Published Version","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"doi":"10.1016/j.celrep.2026.117227","status":"public","abstract":[{"text":"As vertebrates transitioned from water to land, locomotion shifted from undulatory swimming to limb-based movement. How spinal circuits and their cell types evolved to support this transition remains unclear. We leverage frog metamorphosis, which recapitulates this transition within a single organism, to define how spinal circuits generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons. As limbs develop and their movement complexifies, spinal circuits expand in neuron number and subtype diversity. This expansion is most pronounced for V1 inhibitory neurons, which increase ∼70-fold and diversify into transcriptionally distinct subtypes. Disrupting transcription factors defining emerging motor and V1 populations reveals molecular segregation between swim and limb circuits, highlighting the role of subtype diversity in motor coordination. A multifold increase in inhibitory neuron diversity thus underlies the tail-to-limb locomotor transition, providing a framework for spinal circuit adaptation during vertebrate evolution.","lang":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"month":"04","volume":45,"article_type":"original","year":"2026","date_published":"2026-04-28T00:00:00Z","file_date_updated":"2026-05-04T12:20:10Z","date_created":"2026-04-19T22:07:43Z","language":[{"iso":"eng"}],"oa":1,"issue":"4","acknowledgement":"We would like to thank the members of the Sweeney Lab, Mario de Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript. We are also grateful to Tom Jessell and Chris Kintner for their scientific insight and mentorship during the conception of this project. It would also have not been possible without the technical support of the Aquatics and Imaging and Optics Facility support teams (ISTA). We thank Martin Estermann for preparing the initial draft of the graphical abstract and Niki Barolini for the final version. In addition, we thank our funding sources for providing the resources to do these experiments: GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.), Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858 (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC) (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472 (to Z.H.), and Project A.L.S. (to S.B.-M.).","intvolume":"        45","corr_author":"1","external_id":{"pmid":["41964955 "]},"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"pmid":1,"article_number":"117227","publication_status":"published","author":[{"id":"cf391e77-ec3c-11ea-a124-d69323410b58","full_name":"Vijatovic, David","last_name":"Vijatovic","first_name":"David"},{"last_name":"Toma","first_name":"Florina Alexandra ","id":"2f73f876-f128-11eb-9611-b96b5a30cb0e","full_name":"Toma, Florina Alexandra "},{"first_name":"Y","last_name":"Ignatyev","full_name":"Ignatyev, Y"},{"last_name":"Harrington","first_name":"Zoe P","id":"a8144562-32c9-11ee-b5ce-d9800628bda2","full_name":"Harrington, Zoe P","orcid":"0009-0008-0158-4032"},{"full_name":"Sommer, Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph M","last_name":"Sommer"},{"last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"last_name":"Smits","first_name":"Matthijs Geert","id":"7a231d52-e216-11ee-a0bb-8acd55f8f1f0","full_name":"Smits, Matthijs Geert"},{"first_name":"Marco","last_name":"Dalla Vecchia","full_name":"Dalla Vecchia, Marco","id":"02a7a869-ff06-11ed-a87f-86649d6077e5"},{"full_name":"Trevisan, Alexandra J.","first_name":"Alexandra J.","last_name":"Trevisan"},{"first_name":"Phillip","last_name":"Chapman","full_name":"Chapman, Phillip"},{"last_name":"Julseth","first_name":"Mara","id":"1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1","full_name":"Julseth, Mara"},{"last_name":"Brenner-Morton","first_name":"Susan","full_name":"Brenner-Morton, Susan"},{"last_name":"Gabitto","first_name":"Mariano I.","full_name":"Gabitto, Mariano I."},{"full_name":"Dasen, Jeremy S.","first_name":"Jeremy S.","last_name":"Dasen"},{"first_name":"Jay B.","last_name":"Bikoff","full_name":"Bikoff, Jay B."},{"orcid":"0000-0001-9242-5601","full_name":"Sweeney, Lora Beatrice Jaeger","id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney"}],"DOAJ_listed":"1","PlanS_conform":"1","project":[{"grant_number":"101041551","name":"Development and Evolution of Tetrapod Motor Circuits","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","grant_number":"F7814"},{"_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy","grant_number":"CZI01"},{"_id":"bd73af52-d553-11ed-ba76-912049f0ac7a","grant_number":"FTI21-D-046","name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis"}]},{"project":[{"grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"},{"grant_number":"ALTF 1159-2018","name":"Mechanosensation in cell migration: the role of friction forces in cell polarization and directed migration","_id":"269CD5C4-B435-11E9-9278-68D0E5697425"}],"DOAJ_listed":"1","author":[{"first_name":"Ste","last_name":"Tavano","orcid":"0000-0001-9970-7804","full_name":"Tavano, Ste","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Brückner","first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","orcid":"0000-0001-7205-2975","full_name":"Brückner, David"},{"last_name":"Tasciyan","first_name":"Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X"},{"full_name":"Tong, Xin","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","first_name":"Xin","last_name":"Tong"},{"first_name":"Roland","last_name":"Kardos","full_name":"Kardos, Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Alexandra","last_name":"Schauer","orcid":"0000-0001-7659-9142","full_name":"Schauer, Alexandra","id":"30A536BA-F248-11E8-B48F-1D18A9856A87"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"publication_status":"published","tmp":{"image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"pmid":1,"article_number":"115387","external_id":{"pmid":["40057955"],"isi":["001443652700001"]},"corr_author":"1","intvolume":"        44","isi":1,"acknowledgement":"We are grateful to the colleagues who contributed to this work with discussions, technical advice, and feedback on the manuscript: Irene Steccari, David Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship (ALTF 1159-2018).","issue":"3","oa":1,"language":[{"iso":"eng"}],"date_created":"2025-03-16T23:01:24Z","date_published":"2025-03-25T00:00:00Z","file_date_updated":"2025-03-17T10:26:54Z","year":"2025","article_type":"original","volume":44,"month":"03","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"ScienComp"}],"abstract":[{"text":"Cell migration is a fundamental process during embryonic development. Most studies in vivo have focused on the migration of cells using the extracellular matrix (ECM) as their substrate for migration. In contrast, much less is known about how cells migrate on other cells, as found in early embryos when the ECM has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the early zebrafish gastrula use the ectoderm as their substrate for migration. We show that the lateral ectoderm is permissive for the animal-pole-directed migration of LME cells, while the ectoderm at the animal pole halts it. These differences in permissiveness depend on the lateral ectoderm being more cohesive than the animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion as one critical factor in regulating LME migration during zebrafish gastrulation.","lang":"eng"}],"status":"public","publication_identifier":{"eissn":["2211-1247"],"issn":["2639-1856"]},"doi":"10.1016/j.celrep.2025.115387","quality_controlled":"1","scopus_import":"1","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"article_processing_charge":"Yes","type":"journal_article","file":[{"date_updated":"2025-03-17T10:26:54Z","file_name":"2025_CellReports_Tavano.pdf","file_id":"19413","checksum":"57e05dd1598c807af0afdb32cec039d3","success":1,"file_size":9067797,"content_type":"application/pdf","creator":"dernst","access_level":"open_access","relation":"main_file","date_created":"2025-03-17T10:26:54Z"}],"publisher":"Elsevier","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"Bio"}],"publication":"Cell Reports","has_accepted_license":"1","citation":{"ama":"Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>","apa":"Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A., … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>","mla":"Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>, vol. 44, no. 3, 115387, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">10.1016/j.celrep.2025.115387</a>.","ieee":"S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Elsevier, 2025.","short":"S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild, C.-P.J. Heisenberg, Cell Reports 44 (2025).","ista":"Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R, Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation. Cell Reports. 44(3), 115387.","chicago":"Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.115387\">https://doi.org/10.1016/j.celrep.2025.115387</a>."},"_id":"19404","title":"BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation","OA_place":"publisher","OA_type":"gold","date_updated":"2025-10-22T07:00:04Z","day":"25"},{"article_processing_charge":"Yes (in subscription journal)","type":"journal_article","ddc":["580"],"oa_version":"Published Version","scopus_import":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","status":"public","quality_controlled":"1","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"doi":"10.1016/j.celrep.2025.116024","day":"22","OA_type":"hybrid","date_updated":"2025-09-30T14:05:28Z","_id":"20029","title":"Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD","OA_place":"publisher","department":[{"_id":"JiFr"}],"publisher":"Elsevier","file":[{"checksum":"ee03deee47a084b0295251dc49470ad4","file_id":"20067","success":1,"date_updated":"2025-07-22T08:52:17Z","file_name":"2025_CellReports_Guan.pdf","access_level":"open_access","date_created":"2025-07-22T08:52:17Z","relation":"main_file","file_size":37708120,"content_type":"application/pdf","creator":"dernst"}],"citation":{"apa":"Guan, B., Xie, K. X., Du, X. Q., Bai, Y. X., Hao, P. C., Lin, W. H., … Xue, H. W. (2025). Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.116024\">https://doi.org/10.1016/j.celrep.2025.116024</a>","mla":"Guan, Bin, et al. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” <i>Cell Reports</i>, vol. 44, no. 7, 116024, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116024\">10.1016/j.celrep.2025.116024</a>.","ieee":"B. Guan <i>et al.</i>, “Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD,” <i>Cell Reports</i>, vol. 44, no. 7. Elsevier, 2025.","ama":"Guan B, Xie KX, Du XQ, et al. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. <i>Cell Reports</i>. 2025;44(7). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116024\">10.1016/j.celrep.2025.116024</a>","ista":"Guan B, Xie KX, Du XQ, Bai YX, Hao PC, Lin WH, Friml J, Xue HW. 2025. Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Reports. 44(7), 116024.","chicago":"Guan, Bin, Ke Xuan Xie, Xin Qiao Du, Yu Xuan Bai, Peng Chao Hao, Wen Hui Lin, Jiří Friml, and Hong Wei Xue. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting with VATD.” <i>Cell Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.116024\">https://doi.org/10.1016/j.celrep.2025.116024</a>.","short":"B. Guan, K.X. Xie, X.Q. Du, Y.X. Bai, P.C. Hao, W.H. Lin, J. Friml, H.W. Xue, Cell Reports 44 (2025)."},"publication":"Cell Reports","has_accepted_license":"1","pmid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)"},"article_number":"116024","publication_status":"published","external_id":{"isi":["001533244800001"],"pmid":["40668679"]},"author":[{"first_name":"Bin","last_name":"Guan","full_name":"Guan, Bin","id":"56aad729-cca2-11ed-a45a-9b4138991a48"},{"last_name":"Xie","first_name":"Ke Xuan","full_name":"Xie, Ke Xuan"},{"full_name":"Du, Xin Qiao","first_name":"Xin Qiao","last_name":"Du"},{"full_name":"Bai, Yu Xuan","last_name":"Bai","first_name":"Yu Xuan"},{"first_name":"Peng Chao","last_name":"Hao","full_name":"Hao, Peng Chao"},{"full_name":"Lin, Wen Hui","first_name":"Wen Hui","last_name":"Lin"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Xue, Hong Wei","last_name":"Xue","first_name":"Hong Wei"}],"month":"07","abstract":[{"lang":"eng","text":"Vacuolar acidification is crucial for the homeostasis of intracellular pH and the recycling of proteins and nutrients in cells, thereby playing important roles in various physiological processes related to vacuolar function. The key factors regulating vacuolar acidification and underlying mechanisms remain unclear. Here, we report that Arabidopsis phospholipase Dζ2 (PLDζ2) promotes the acidification of the vacuolar lumen to stimulate autophagic degradation under phosphorus deficiency. The pldζ2 mutant massively accumulates autophagic structures while exhibiting premature leaf senescence under nutrient starvation. Impaired autophagic flux, lytic vacuole morphology, and lytic degradation in pldζ2 indicate that PLDζ2 regulates autophagy by affecting the vacuolar function. PLDζ2 locates in both tonoplast and cytoplasm. Genetic, structural, and biochemical studies demonstrate that PLDζ2 directly interacts with vacuolar-type ATPase (V-ATPase) subunit D (VATD) to promote vacuolar acidification and autophagy under phosphorus starvation. These findings reveal the importance of V-ATPase and vacuolar pH in autophagic activity and provide clues in elucidating the regulatory mechanism of vacuolar acidification."}],"volume":44,"date_created":"2025-07-20T22:02:01Z","year":"2025","article_type":"original","date_published":"2025-07-22T00:00:00Z","file_date_updated":"2025-07-22T08:52:17Z","intvolume":"        44","oa":1,"issue":"7","isi":1,"language":[{"iso":"eng"}],"acknowledgement":"The study was supported by National Natural Science Foundation of China (NSFC, 92354301, 32230011, 32200274, and 91954206). The computations were run on the Siyuan-1 cluster supported by the Center for High-Performance Computing at Shanghai Jiao Tong University."},{"volume":44,"abstract":[{"lang":"eng","text":"The hippocampus, critical for learning and memory, is dogmatically described as a trisynaptic circuit where dentate gyrus granule cells (GCs), CA3 pyramidal neurons (PNs), and CA1 PNs are serially connected. However, CA3 also forms an autoassociative network, and its PNs have diverse morphologies, intrinsic properties, and GC input levels. How PN subtypes compose this recurrent network is unknown. To determine the synaptic arrangement of identified CA3 PNs, we combine multicellular patch-clamp recording and post hoc morphological analysis in mouse hippocampal slices. PNs can be divided into distinct “superficial” and “deep” subclasses, the latter including previously reported “athorny” cells. Subclasses have distinct input-output transformations and asymmetric connectivity, which is more abundant from superficial to deep PNs, splitting CA3 locally into two parallel recurrent networks. Coincident spontaneous inhibition occurs frequently within but not between subclasses, implying subclass-specific inhibitory innervation. Our results suggest two separately controlled sublayers for parallel information processing in hippocampal CA3."}],"month":"08","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"oa":1,"issue":"8","isi":1,"acknowledgement":"We thank Andrea Navas-Olive and Rebecca J. Morse-Mora for critically reading an earlier version of the manuscript. We also thank Florian Marr and Christina Altmutter for excellent technical assistance, Alois Schlögl for programming and data-handling assistance, Todor Asenov for technical support, and Eleftheria Kralli-Beller for manuscript editing. This research was supported by the Scientific Services Units (SSUs) of ISTA. We are particularly grateful for assistance from the Imaging and Optics Facility, Preclinical Facility, Lab Support Facility, and Miba Machine Shop. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 692692 to P.J., Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635 to J.F.W., and an ISTplus Fellowship through Marie Skłodowska-Curie grant agreement no. 754411 to V.V.-B.), the Austrian Science Fund (P 36232-B, PAT 4178023, and Cluster of Excellence 10.55776/COE16 to P.J.), and a CONACyT fellowship (289638 to V.V.-B.) and was supported by a non-stipendiary EMBO fellowship (ALTF 756–2020 to J.F.W.).","language":[{"iso":"eng"}],"intvolume":"        44","date_published":"2025-08-01T00:00:00Z","file_date_updated":"2025-08-04T06:53:07Z","article_type":"original","year":"2025","date_created":"2025-08-03T22:01:30Z","author":[{"id":"63836096-4690-11EA-BD4E-32803DDC885E","full_name":"Watson, Jake","orcid":"0000-0002-8698-3823","last_name":"Watson","first_name":"Jake"},{"first_name":"Victor M","last_name":"Vargas Barroso","full_name":"Vargas Barroso, Victor M","id":"2F55A9DE-F248-11E8-B48F-1D18A9856A87"},{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","last_name":"Jonas","first_name":"Peter M"}],"external_id":{"isi":["001544472300002"]},"corr_author":"1","publication_status":"published","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_number":"116080","DOAJ_listed":"1","PlanS_conform":"1","project":[{"_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","call_identifier":"H2020","grant_number":"692692"},{"name":"Synaptic computations of the hippocampal CA3 circuitry","call_identifier":"H2020","grant_number":"101026635","_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9"},{"_id":"bd88be38-d553-11ed-ba76-81d5a70a6ef5","grant_number":"P36232","name":"Mechanisms of GABA release in hippocampal circuits"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","call_identifier":"H2020"}],"date_updated":"2025-09-30T14:12:02Z","OA_type":"gold","day":"01","has_accepted_license":"1","publication":"Cell Reports","citation":{"chicago":"Watson, Jake, Victor M Vargas Barroso, and Peter M Jonas. “Cell-Specific Wiring Routes Information Flow through Hippocampal CA3.” <i>Cell Reports</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.celrep.2025.116080\">https://doi.org/10.1016/j.celrep.2025.116080</a>.","ista":"Watson J, Vargas Barroso VM, Jonas PM. 2025. Cell-specific wiring routes information flow through hippocampal CA3. Cell Reports. 44(8), 116080.","short":"J. Watson, V.M. Vargas Barroso, P.M. Jonas, Cell Reports 44 (2025).","ieee":"J. Watson, V. M. Vargas Barroso, and P. M. Jonas, “Cell-specific wiring routes information flow through hippocampal CA3,” <i>Cell Reports</i>, vol. 44, no. 8. Elsevier, 2025.","mla":"Watson, Jake, et al. “Cell-Specific Wiring Routes Information Flow through Hippocampal CA3.” <i>Cell Reports</i>, vol. 44, no. 8, 116080, Elsevier, 2025, doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116080\">10.1016/j.celrep.2025.116080</a>.","apa":"Watson, J., Vargas Barroso, V. M., &#38; Jonas, P. M. (2025). Cell-specific wiring routes information flow through hippocampal CA3. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2025.116080\">https://doi.org/10.1016/j.celrep.2025.116080</a>","ama":"Watson J, Vargas Barroso VM, Jonas PM. Cell-specific wiring routes information flow through hippocampal CA3. <i>Cell Reports</i>. 2025;44(8). doi:<a href=\"https://doi.org/10.1016/j.celrep.2025.116080\">10.1016/j.celrep.2025.116080</a>"},"file":[{"file_id":"20106","checksum":"556ff9760661ecd23949d75031043b1f","success":1,"date_updated":"2025-08-04T06:53:07Z","file_name":"2025_CellReports_Watson.pdf","access_level":"open_access","relation":"main_file","date_created":"2025-08-04T06:53:07Z","content_type":"application/pdf","file_size":27695214,"creator":"dernst"}],"department":[{"_id":"PeJo"}],"publisher":"Elsevier","OA_place":"publisher","_id":"20099","title":"Cell-specific wiring routes information flow through hippocampal CA3","ddc":["570"],"type":"journal_article","article_processing_charge":"Yes","publication_identifier":{"issn":["2639-1856"],"eissn":["2211-1247"]},"doi":"10.1016/j.celrep.2025.116080","quality_controlled":"1","status":"public","ec_funded":1,"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa_version":"Published Version","scopus_import":"1"}]