---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '21744'
abstract:
- lang: eng
  text: The paraventricular hypothalamus (PVH) controls behavioral and physiologic
    processes, including appetite, social behavior, autonomic outflow, and pituitary
    hormone secretion. However, molecular markers for centrally projecting PVH neuron
    populations remain largely undefined, and a complete census of PVH cell types
    has not been established. Therefore, we performed extensive single-cell/nucleus
    RNA sequencing to catalog PVH neuron subtypes and multiplexed error-robust fluorescence
    in situ hybridization (MERFISH) to map them spatially. Our spatial transcriptomic
    atlas resolves 26 Sim1+ and 29 GABAergic neuron populations from the PVH and surrounding
    areas. Additionally, projection-based profiling identified neurons that project
    to the parabrachial region (PB) and spinal cord, helping to determine PVH populations
    that regulate satiety and sympathetic nervous system activity, respectively. Notably,
    activation of PB-projecting PVH neurons expressing Brs3 reduces food intake, and
    silencing them causes obesity. Together, this atlas contributes high-resolution
    PVH spatial and circuit-based gene expression profiles, representing a valuable
    resource for the field of homeostasis.
acknowledgement: "We would like to thank Drs. Mark Andermann, Joel Geerling, and Clifford\r\nSaper,
  as well as the Lowell, Tsai, and Resch laboratories for helpful discussions;\r\nAlysia
  Berns, Jia Yu, and Yanfang Li for technical support; the BNORC\r\nFunctional Genomics
  and Bioinformatics Core (P30DK046200) and the Iowa\r\nInstitute for Human Genetics
  Genomics Division (IIHG, RRID: SCR_023422)\r\nfor helpful discussions and technical
  assistance with sc/snRNA-seq; Zachary\r\nNiziolek and the Bauer Core Facility at
  Harvard University, the BIDMC Flow Cytometry\r\nCore, and Heath Vignes, Michael
  Shey, and Thomas Kaufman of the\r\nFlow Cytometry Facility at the University of
  Iowa Carver College of Medicine\r\nfor helpful discussions and technical support;
  the ICCB-Longwood Screening\r\nFacility of Harvard Medical School for assistance
  with the snRNA-seq\r\nexperiments; Dr. Sayak Mitter and Vizgen support for technical
  assistance\r\nwith the MERSCOPE platform; and Mara Jendro and Li-Chun (Queena) Lin\r\nfor
  their assistance with MERSCOPE experiments within the Iowa\r\nNeuroBank Core in
  the Iowa Neuroscience Institute at the University of Iowa\r\nCarver College of Medicine.
  This research was funded by the following NIH\r\ngrants to L.T.T.: R01DK128406;
  to B.B.L.: R01DK075632, R01DK134427,\r\nand R01DK096010; to J.M.R.: R00HL144923
  and R01NS141072; and to M.C.M.: F31HL170784; T.C.B. and M.C.M. were supported by
  a pharmacological\r\nsciences predoctoral training grant T32GM144636. Additional
  funding\r\nto J.M.R. came from the American Heart Association (AHA 935362), a University\r\nof
  Iowa Fraternal Order of Eagles Diabetes Research Center Pilot and\r\nFeasibility
  Catalyst Grant, and an Iowa Neuroscience Institute Early Stage\r\nInvestigator award
  from the Carver Trust. Y.L. was supported by a predoctoral\r\nfellowship from the
  American Heart Association (AHA 25PRE1372983). A.M.D.\r\nwas supported by a postdoctoral
  fellowship from the Charles A. King Trust."
article_number: '116904'
article_processing_charge: Yes
article_type: original
author:
- first_name: Yuxi
  full_name: Li, Yuxi
  last_name: Li
- first_name: Trevor C.
  full_name: Butler, Trevor C.
  last_name: Butler
- first_name: Stefano
  full_name: Nardone, Stefano
  last_name: Nardone
- first_name: Christopher L.
  full_name: Jacobs, Christopher L.
  last_name: Jacobs
- first_name: Amelia May Barnett
  full_name: Douglass, Amelia May Barnett
  id: de5f6fda-80fb-11ef-996f-a8c4ecd8e289
  last_name: Douglass
  orcid: 0000-0001-5398-6473
- first_name: Joseph C.
  full_name: Madara, Joseph C.
  last_name: Madara
- first_name: Miriam C.
  full_name: McDonough, Miriam C.
  last_name: McDonough
- first_name: Jenkang
  full_name: Tao, Jenkang
  last_name: Tao
- first_name: Elijah D.
  full_name: Lowenstein, Elijah D.
  last_name: Lowenstein
- first_name: Luhong
  full_name: Wang, Luhong
  last_name: Wang
- first_name: Deepti
  full_name: Pant, Deepti
  last_name: Pant
- first_name: Samuel J.
  full_name: Walker, Samuel J.
  last_name: Walker
- first_name: Annette
  full_name: Wang, Annette
  last_name: Wang
- first_name: Harini
  full_name: Srinivasan, Harini
  last_name: Srinivasan
- first_name: Zongfang
  full_name: Yang, Zongfang
  last_name: Yang
- first_name: John N.
  full_name: Campbell, John N.
  last_name: Campbell
- first_name: Linus T.
  full_name: Tsai, Linus T.
  last_name: Tsai
- first_name: Bradford B.
  full_name: Lowell, Bradford B.
  last_name: Lowell
- first_name: Jon M.
  full_name: Resch, Jon M.
  last_name: Resch
citation:
  ama: Li Y, Butler TC, Nardone S, et al. A spatial and projection-based transcriptomic
    atlas of paraventricular hypothalamic cell types. <i>Cell Reports</i>. 2026;45(2).
    doi:<a href="https://doi.org/10.1016/j.celrep.2025.116904">10.1016/j.celrep.2025.116904</a>
  apa: Li, Y., Butler, T. C., Nardone, S., Jacobs, C. L., Douglass, A. M., Madara,
    J. C., … Resch, J. M. (2026). A spatial and projection-based transcriptomic atlas
    of paraventricular hypothalamic cell types. <i>Cell Reports</i>. Elsevier. <a
    href="https://doi.org/10.1016/j.celrep.2025.116904">https://doi.org/10.1016/j.celrep.2025.116904</a>
  chicago: Li, Yuxi, Trevor C. Butler, Stefano Nardone, Christopher L. Jacobs, Amelia
    M. Douglass, Joseph C. Madara, Miriam C. McDonough, et al. “A Spatial and Projection-Based
    Transcriptomic Atlas of Paraventricular Hypothalamic Cell Types.” <i>Cell Reports</i>.
    Elsevier, 2026. <a href="https://doi.org/10.1016/j.celrep.2025.116904">https://doi.org/10.1016/j.celrep.2025.116904</a>.
  ieee: Y. Li <i>et al.</i>, “A spatial and projection-based transcriptomic atlas
    of paraventricular hypothalamic cell types,” <i>Cell Reports</i>, vol. 45, no.
    2. Elsevier, 2026.
  ista: Li Y, Butler TC, Nardone S, Jacobs CL, Douglass AM, Madara JC, McDonough MC,
    Tao J, Lowenstein ED, Wang L, Pant D, Walker SJ, Wang A, Srinivasan H, Yang Z,
    Campbell JN, Tsai LT, Lowell BB, Resch JM. 2026. A spatial and projection-based
    transcriptomic atlas of paraventricular hypothalamic cell types. Cell Reports.
    45(2), 116904.
  mla: Li, Yuxi, et al. “A Spatial and Projection-Based Transcriptomic Atlas of Paraventricular
    Hypothalamic Cell Types.” <i>Cell Reports</i>, vol. 45, no. 2, 116904, Elsevier,
    2026, doi:<a href="https://doi.org/10.1016/j.celrep.2025.116904">10.1016/j.celrep.2025.116904</a>.
  short: Y. Li, T.C. Butler, S. Nardone, C.L. Jacobs, A.M. Douglass, J.C. Madara,
    M.C. McDonough, J. Tao, E.D. Lowenstein, L. Wang, D. Pant, S.J. Walker, A. Wang,
    H. Srinivasan, Z. Yang, J.N. Campbell, L.T. Tsai, B.B. Lowell, J.M. Resch, Cell
    Reports 45 (2026).
date_created: 2026-04-16T13:51:29Z
date_published: 2026-02-24T00:00:00Z
date_updated: 2026-05-04T12:00:31Z
day: '24'
ddc:
- '570'
department:
- _id: AmDo
doi: 10.1016/j.celrep.2025.116904
external_id:
  pmid:
  - '41581146'
file:
- access_level: open_access
  checksum: 82098dd9d0ca609119f9f2c6beb4fc1e
  content_type: application/pdf
  creator: dernst
  date_created: 2026-05-04T11:58:51Z
  date_updated: 2026-05-04T11:58:51Z
  file_id: '21793'
  file_name: 2026_CellReports_Li.pdf
  file_size: 38532865
  relation: main_file
  success: 1
file_date_updated: 2026-05-04T11:58:51Z
has_accepted_license: '1'
intvolume: '        45'
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic
  cell types
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21746'
abstract:
- lang: eng
  text: As vertebrates transitioned from water to land, locomotion shifted from undulatory
    swimming to limb-based movement. How spinal circuits and their cell types evolved
    to support this transition remains unclear. We leverage frog metamorphosis, which
    recapitulates this transition within a single organism, to define how spinal circuits
    generate aquatic versus terrestrial motor patterns. At swim stages, spinal architecture
    is uniform, with a transcriptionally and anatomically homogeneous motor and interneurons.
    As limbs develop and their movement complexifies, spinal circuits expand in neuron
    number and subtype diversity. This expansion is most pronounced for V1 inhibitory
    neurons, which increase ∼70-fold and diversify into transcriptionally distinct
    subtypes. Disrupting transcription factors defining emerging motor and V1 populations
    reveals molecular segregation between swim and limb circuits, highlighting the
    role of subtype diversity in motor coordination. A multifold increase in inhibitory
    neuron diversity thus underlies the tail-to-limb locomotor transition, providing
    a framework for spinal circuit adaptation during vertebrate evolution.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We would like to thank the members of the Sweeney Lab, Mario de
  Bono, Michael Forsthofer, Katharina Lust, and Meital Oren, for comments on the manuscript.
  We are also grateful to Tom Jessell and Chris Kintner for their scientific insight
  and mentorship during the conception of this project. It would also have not been
  possible without the technical support of the Aquatics and Imaging and Optics Facility
  support teams (ISTA). We thank Martin Estermann for preparing the initial draft
  of the graphical abstract and Niki Barolini for the final version. In addition,
  we thank our funding sources for providing the resources to do these experiments:
  GFF NÖ FTI Strategy Lower Austria dissertation grant FT121-D-046 (to D.V.), Horizon
  Europe ERC starting grant 101041551 (to Y.I., L.B.S., F.A.T., and D.V.), Special
  Research Program (SFB) of the Austrian Science Fund (FWF) project F7814-B (to L.B.S.),
  Austrian Science Fund (FWF) 10.55776/COE16 (to Y.I. and L.B.S.), NINDS 5R35NS116858
  (to J.S.D.), CZI grant DAF2020-225401 (DOI) 10.37921/120055ratwvi (to R.H.), NIH
  grant R01NS123116 (to J.B.B.), American Lebanese Syrian Associated Charities (ALSAC)
  (to J.B.B.), German Academic Exchange Service (DAAD) IFI grant 57515251-91853472
  (to Z.H.), and Project A.L.S. (to S.B.-M.).'
article_number: '117227'
article_processing_charge: Yes
article_type: original
author:
- first_name: David
  full_name: Vijatovic, David
  id: cf391e77-ec3c-11ea-a124-d69323410b58
  last_name: Vijatovic
- first_name: 'Florina Alexandra '
  full_name: 'Toma, Florina Alexandra '
  id: 2f73f876-f128-11eb-9611-b96b5a30cb0e
  last_name: Toma
- first_name: Y
  full_name: Ignatyev, Y
  last_name: Ignatyev
- first_name: Zoe P
  full_name: Harrington, Zoe P
  id: a8144562-32c9-11ee-b5ce-d9800628bda2
  last_name: Harrington
  orcid: 0009-0008-0158-4032
- first_name: Christoph M
  full_name: Sommer, Christoph M
  id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
  last_name: Sommer
  orcid: 0000-0003-1216-9105
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Matthijs Geert
  full_name: Smits, Matthijs Geert
  id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0
  last_name: Smits
- first_name: Marco
  full_name: Dalla Vecchia, Marco
  id: 02a7a869-ff06-11ed-a87f-86649d6077e5
  last_name: Dalla Vecchia
- first_name: Alexandra J.
  full_name: Trevisan, Alexandra J.
  last_name: Trevisan
- first_name: Phillip
  full_name: Chapman, Phillip
  last_name: Chapman
- first_name: Mara
  full_name: Julseth, Mara
  id: 1cf464b2-dc7d-11ea-9b2f-f9b1aa9417d1
  last_name: Julseth
- first_name: Susan
  full_name: Brenner-Morton, Susan
  last_name: Brenner-Morton
- first_name: Mariano I.
  full_name: Gabitto, Mariano I.
  last_name: Gabitto
- first_name: Jeremy S.
  full_name: Dasen, Jeremy S.
  last_name: Dasen
- first_name: Jay B.
  full_name: Bikoff, Jay B.
  last_name: Bikoff
- first_name: Lora Beatrice Jaeger
  full_name: Sweeney, Lora Beatrice Jaeger
  id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
  last_name: Sweeney
  orcid: 0000-0001-9242-5601
citation:
  ama: Vijatovic D, Toma FA, Ignatyev Y, et al. Multifold increase in spinal inhibitory
    cell types with emergence of limb movement. <i>Cell Reports</i>. 2026;45(4). doi:<a
    href="https://doi.org/10.1016/j.celrep.2026.117227">10.1016/j.celrep.2026.117227</a>
  apa: Vijatovic, D., Toma, F. A., Ignatyev, Y., Harrington, Z. P., Sommer, C. M.,
    Hauschild, R., … Sweeney, L. B. (2026). Multifold increase in spinal inhibitory
    cell types with emergence of limb movement. <i>Cell Reports</i>. Elsevier. <a
    href="https://doi.org/10.1016/j.celrep.2026.117227">https://doi.org/10.1016/j.celrep.2026.117227</a>
  chicago: Vijatovic, David, Florina Alexandra  Toma, Y Ignatyev, Zoe P Harrington,
    Christoph M Sommer, Robert Hauschild, Matthijs Geert Smits, et al. “Multifold
    Increase in Spinal Inhibitory Cell Types with Emergence of Limb Movement.” <i>Cell
    Reports</i>. Elsevier, 2026. <a href="https://doi.org/10.1016/j.celrep.2026.117227">https://doi.org/10.1016/j.celrep.2026.117227</a>.
  ieee: D. Vijatovic <i>et al.</i>, “Multifold increase in spinal inhibitory cell
    types with emergence of limb movement,” <i>Cell Reports</i>, vol. 45, no. 4. Elsevier,
    2026.
  ista: Vijatovic D, Toma FA, Ignatyev Y, Harrington ZP, Sommer CM, Hauschild R, Smits
    MG, Dalla Vecchia M, Trevisan AJ, Chapman P, Julseth M, Brenner-Morton S, Gabitto
    MI, Dasen JS, Bikoff JB, Sweeney LB. 2026. Multifold increase in spinal inhibitory
    cell types with emergence of limb movement. Cell Reports. 45(4), 117227.
  mla: Vijatovic, David, et al. “Multifold Increase in Spinal Inhibitory Cell Types
    with Emergence of Limb Movement.” <i>Cell Reports</i>, vol. 45, no. 4, 117227,
    Elsevier, 2026, doi:<a href="https://doi.org/10.1016/j.celrep.2026.117227">10.1016/j.celrep.2026.117227</a>.
  short: D. Vijatovic, F.A. Toma, Y. Ignatyev, Z.P. Harrington, C.M. Sommer, R. Hauschild,
    M.G. Smits, M. Dalla Vecchia, A.J. Trevisan, P. Chapman, M. Julseth, S. Brenner-Morton,
    M.I. Gabitto, J.S. Dasen, J.B. Bikoff, L.B. Sweeney, Cell Reports 45 (2026).
corr_author: '1'
date_created: 2026-04-19T22:07:43Z
date_published: 2026-04-28T00:00:00Z
date_updated: 2026-05-04T12:27:06Z
day: '28'
ddc:
- '570'
department:
- _id: LoSw
- _id: GradSch
- _id: TiVo
- _id: Bio
- _id: NiBa
doi: 10.1016/j.celrep.2026.117227
external_id:
  pmid:
  - '41964955 '
file:
- access_level: open_access
  checksum: 0d26cdb5b8d8dec3a911d8261a65cdef
  content_type: application/pdf
  creator: dernst
  date_created: 2026-05-04T12:20:10Z
  date_updated: 2026-05-04T12:20:10Z
  file_id: '21795'
  file_name: 2026_CellReports_Vijatovic.pdf
  file_size: 14925958
  relation: main_file
  success: 1
file_date_updated: 2026-05-04T12:20:10Z
has_accepted_license: '1'
intvolume: '        45'
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
  grant_number: '101041551'
  name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
  grant_number: F7814
  name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
    transition: cell type to connection diversity'
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
  grant_number: CZI01
  name: Tools for automation and feedback microscopy
- _id: bd73af52-d553-11ed-ba76-912049f0ac7a
  grant_number: FTI21-D-046
  name: Development of V1 interneuron diversity during swim-to-walk transition of
    Xenopus metamorphosis
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Multifold increase in spinal inhibitory cell types with emergence of limb movement
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19404'
abstract:
- lang: eng
  text: Cell migration is a fundamental process during embryonic development. Most
    studies in vivo have focused on the migration of cells using the extracellular
    matrix (ECM) as their substrate for migration. In contrast, much less is known
    about how cells migrate on other cells, as found in early embryos when the ECM
    has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the
    early zebrafish gastrula use the ectoderm as their substrate for migration. We
    show that the lateral ectoderm is permissive for the animal-pole-directed migration
    of LME cells, while the ectoderm at the animal pole halts it. These differences
    in permissiveness depend on the lateral ectoderm being more cohesive than the
    animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling
    within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion
    as one critical factor in regulating LME migration during zebrafish gastrulation.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: ScienComp
acknowledgement: 'We are grateful to the colleagues who contributed to this work with
  discussions, technical advice, and feedback on the manuscript: Irene Steccari, David
  Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian
  Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the
  Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing
  Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter
  Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was
  supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral
  Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship
  (ALTF 1159-2018).'
article_number: '115387'
article_processing_charge: Yes
article_type: original
author:
- first_name: Ste
  full_name: Tavano, Ste
  id: 2F162F0C-F248-11E8-B48F-1D18A9856A87
  last_name: Tavano
  orcid: 0000-0001-9970-7804
- first_name: David
  full_name: Brückner, David
  id: e1e86031-6537-11eb-953a-f7ab92be508d
  last_name: Brückner
  orcid: 0000-0001-7205-2975
- first_name: Saren
  full_name: Tasciyan, Saren
  id: 4323B49C-F248-11E8-B48F-1D18A9856A87
  last_name: Tasciyan
  orcid: 0000-0003-1671-393X
- first_name: Xin
  full_name: Tong, Xin
  id: 50F65CDC-AA30-11E9-A72B-8A12E6697425
  last_name: Tong
- first_name: Roland
  full_name: Kardos, Roland
  id: 4039350E-F248-11E8-B48F-1D18A9856A87
  last_name: Kardos
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm
    tissue material properties modulates lateral mesendoderm cell migration during
    early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href="https://doi.org/10.1016/j.celrep.2025.115387">10.1016/j.celrep.2025.115387</a>
  apa: Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A.,
    … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material
    properties modulates lateral mesendoderm cell migration during early zebrafish
    gastrulation. <i>Cell Reports</i>. Elsevier. <a href="https://doi.org/10.1016/j.celrep.2025.115387">https://doi.org/10.1016/j.celrep.2025.115387</a>
  chicago: Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra
    Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning
    of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration
    during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Elsevier, 2025. <a
    href="https://doi.org/10.1016/j.celrep.2025.115387">https://doi.org/10.1016/j.celrep.2025.115387</a>.
  ieee: S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material
    properties modulates lateral mesendoderm cell migration during early zebrafish
    gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Elsevier, 2025.
  ista: Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R,
    Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties
    modulates lateral mesendoderm cell migration during early zebrafish gastrulation.
    Cell Reports. 44(3), 115387.
  mla: Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties
    Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.”
    <i>Cell Reports</i>, vol. 44, no. 3, 115387, Elsevier, 2025, doi:<a href="https://doi.org/10.1016/j.celrep.2025.115387">10.1016/j.celrep.2025.115387</a>.
  short: S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild,
    C.-P.J. Heisenberg, Cell Reports 44 (2025).
corr_author: '1'
date_created: 2025-03-16T23:01:24Z
date_published: 2025-03-25T00:00:00Z
date_updated: 2025-10-22T07:00:04Z
day: '25'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: MiSi
- _id: Bio
doi: 10.1016/j.celrep.2025.115387
external_id:
  isi:
  - '001443652700001'
  pmid:
  - '40057955'
file:
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  creator: dernst
  date_created: 2025-03-17T10:26:54Z
  date_updated: 2025-03-17T10:26:54Z
  file_id: '19413'
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  success: 1
file_date_updated: 2025-03-17T10:26:54Z
has_accepted_license: '1'
intvolume: '        44'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 34e2a5b5-11ca-11ed-8bc3-b2265616ef0b
  grant_number: ALTF 343-2022
  name: A mechano-chemical theory for stem cell fate decisions in organoid development
- _id: 269CD5C4-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 1159-2018
  name: 'Mechanosensation in cell migration: the role of friction forces in cell polarization
    and directed migration'
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: BMP-dependent patterning of ectoderm tissue material properties modulates lateral
  mesendoderm cell migration during early zebrafish gastrulation
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '20029'
abstract:
- lang: eng
  text: Vacuolar acidification is crucial for the homeostasis of intracellular pH
    and the recycling of proteins and nutrients in cells, thereby playing important
    roles in various physiological processes related to vacuolar function. The key
    factors regulating vacuolar acidification and underlying mechanisms remain unclear.
    Here, we report that Arabidopsis phospholipase Dζ2 (PLDζ2) promotes the acidification
    of the vacuolar lumen to stimulate autophagic degradation under phosphorus deficiency.
    The pldζ2 mutant massively accumulates autophagic structures while exhibiting
    premature leaf senescence under nutrient starvation. Impaired autophagic flux,
    lytic vacuole morphology, and lytic degradation in pldζ2 indicate that PLDζ2 regulates
    autophagy by affecting the vacuolar function. PLDζ2 locates in both tonoplast
    and cytoplasm. Genetic, structural, and biochemical studies demonstrate that PLDζ2
    directly interacts with vacuolar-type ATPase (V-ATPase) subunit D (VATD) to promote
    vacuolar acidification and autophagy under phosphorus starvation. These findings
    reveal the importance of V-ATPase and vacuolar pH in autophagic activity and provide
    clues in elucidating the regulatory mechanism of vacuolar acidification.
acknowledgement: The study was supported by National Natural Science Foundation of
  China (NSFC, 92354301, 32230011, 32200274, and 91954206). The computations were
  run on the Siyuan-1 cluster supported by the Center for High-Performance Computing
  at Shanghai Jiao Tong University.
article_number: '116024'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Bin
  full_name: Guan, Bin
  id: 56aad729-cca2-11ed-a45a-9b4138991a48
  last_name: Guan
- first_name: Ke Xuan
  full_name: Xie, Ke Xuan
  last_name: Xie
- first_name: Xin Qiao
  full_name: Du, Xin Qiao
  last_name: Du
- first_name: Yu Xuan
  full_name: Bai, Yu Xuan
  last_name: Bai
- first_name: Peng Chao
  full_name: Hao, Peng Chao
  last_name: Hao
- first_name: Wen Hui
  full_name: Lin, Wen Hui
  last_name: Lin
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Hong Wei
  full_name: Xue, Hong Wei
  last_name: Xue
citation:
  ama: Guan B, Xie KX, Du XQ, et al. Arabidopsis phospholipase Dζ2 facilitates vacuolar
    acidification and autophagy under phosphorus starvation by interacting with VATD.
    <i>Cell Reports</i>. 2025;44(7). doi:<a href="https://doi.org/10.1016/j.celrep.2025.116024">10.1016/j.celrep.2025.116024</a>
  apa: Guan, B., Xie, K. X., Du, X. Q., Bai, Y. X., Hao, P. C., Lin, W. H., … Xue,
    H. W. (2025). Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification
    and autophagy under phosphorus starvation by interacting with VATD. <i>Cell Reports</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.celrep.2025.116024">https://doi.org/10.1016/j.celrep.2025.116024</a>
  chicago: Guan, Bin, Ke Xuan Xie, Xin Qiao Du, Yu Xuan Bai, Peng Chao Hao, Wen Hui
    Lin, Jiří Friml, and Hong Wei Xue. “Arabidopsis Phospholipase Dζ2 Facilitates
    Vacuolar Acidification and Autophagy under Phosphorus Starvation by Interacting
    with VATD.” <i>Cell Reports</i>. Elsevier, 2025. <a href="https://doi.org/10.1016/j.celrep.2025.116024">https://doi.org/10.1016/j.celrep.2025.116024</a>.
  ieee: B. Guan <i>et al.</i>, “Arabidopsis phospholipase Dζ2 facilitates vacuolar
    acidification and autophagy under phosphorus starvation by interacting with VATD,”
    <i>Cell Reports</i>, vol. 44, no. 7. Elsevier, 2025.
  ista: Guan B, Xie KX, Du XQ, Bai YX, Hao PC, Lin WH, Friml J, Xue HW. 2025. Arabidopsis
    phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus
    starvation by interacting with VATD. Cell Reports. 44(7), 116024.
  mla: Guan, Bin, et al. “Arabidopsis Phospholipase Dζ2 Facilitates Vacuolar Acidification
    and Autophagy under Phosphorus Starvation by Interacting with VATD.” <i>Cell Reports</i>,
    vol. 44, no. 7, 116024, Elsevier, 2025, doi:<a href="https://doi.org/10.1016/j.celrep.2025.116024">10.1016/j.celrep.2025.116024</a>.
  short: B. Guan, K.X. Xie, X.Q. Du, Y.X. Bai, P.C. Hao, W.H. Lin, J. Friml, H.W.
    Xue, Cell Reports 44 (2025).
date_created: 2025-07-20T22:02:01Z
date_published: 2025-07-22T00:00:00Z
date_updated: 2025-09-30T14:05:28Z
day: '22'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.celrep.2025.116024
external_id:
  isi:
  - '001533244800001'
  pmid:
  - '40668679'
file:
- access_level: open_access
  checksum: ee03deee47a084b0295251dc49470ad4
  content_type: application/pdf
  creator: dernst
  date_created: 2025-07-22T08:52:17Z
  date_updated: 2025-07-22T08:52:17Z
  file_id: '20067'
  file_name: 2025_CellReports_Guan.pdf
  file_size: 37708120
  relation: main_file
  success: 1
file_date_updated: 2025-07-22T08:52:17Z
has_accepted_license: '1'
intvolume: '        44'
isi: 1
issue: '7'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy
  under phosphorus starvation by interacting with VATD
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 44
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20099'
abstract:
- lang: eng
  text: The hippocampus, critical for learning and memory, is dogmatically described
    as a trisynaptic circuit where dentate gyrus granule cells (GCs), CA3 pyramidal
    neurons (PNs), and CA1 PNs are serially connected. However, CA3 also forms an
    autoassociative network, and its PNs have diverse morphologies, intrinsic properties,
    and GC input levels. How PN subtypes compose this recurrent network is unknown.
    To determine the synaptic arrangement of identified CA3 PNs, we combine multicellular
    patch-clamp recording and post hoc morphological analysis in mouse hippocampal
    slices. PNs can be divided into distinct “superficial” and “deep” subclasses,
    the latter including previously reported “athorny” cells. Subclasses have distinct
    input-output transformations and asymmetric connectivity, which is more abundant
    from superficial to deep PNs, splitting CA3 locally into two parallel recurrent
    networks. Coincident spontaneous inhibition occurs frequently within but not between
    subclasses, implying subclass-specific inhibitory innervation. Our results suggest
    two separately controlled sublayers for parallel information processing in hippocampal
    CA3.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
- _id: LifeSc
- _id: M-Shop
acknowledgement: We thank Andrea Navas-Olive and Rebecca J. Morse-Mora for critically
  reading an earlier version of the manuscript. We also thank Florian Marr and Christina
  Altmutter for excellent technical assistance, Alois Schlögl for programming and
  data-handling assistance, Todor Asenov for technical support, and Eleftheria Kralli-Beller
  for manuscript editing. This research was supported by the Scientific Services Units
  (SSUs) of ISTA. We are particularly grateful for assistance from the Imaging and
  Optics Facility, Preclinical Facility, Lab Support Facility, and Miba Machine Shop.
  The project received funding from the European Research Council (ERC) under the
  European Union’s Horizon 2020 research and innovation program (grant agreement no.
  692692 to P.J., Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635
  to J.F.W., and an ISTplus Fellowship through Marie Skłodowska-Curie grant agreement
  no. 754411 to V.V.-B.), the Austrian Science Fund (P 36232-B, PAT 4178023, and Cluster
  of Excellence 10.55776/COE16 to P.J.), and a CONACyT fellowship (289638 to V.V.-B.)
  and was supported by a non-stipendiary EMBO fellowship (ALTF 756–2020 to J.F.W.).
article_number: '116080'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jake
  full_name: Watson, Jake
  id: 63836096-4690-11EA-BD4E-32803DDC885E
  last_name: Watson
  orcid: 0000-0002-8698-3823
- first_name: Victor M
  full_name: Vargas Barroso, Victor M
  id: 2F55A9DE-F248-11E8-B48F-1D18A9856A87
  last_name: Vargas Barroso
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: Watson J, Vargas Barroso VM, Jonas PM. Cell-specific wiring routes information
    flow through hippocampal CA3. <i>Cell Reports</i>. 2025;44(8). doi:<a href="https://doi.org/10.1016/j.celrep.2025.116080">10.1016/j.celrep.2025.116080</a>
  apa: Watson, J., Vargas Barroso, V. M., &#38; Jonas, P. M. (2025). Cell-specific
    wiring routes information flow through hippocampal CA3. <i>Cell Reports</i>. Elsevier.
    <a href="https://doi.org/10.1016/j.celrep.2025.116080">https://doi.org/10.1016/j.celrep.2025.116080</a>
  chicago: Watson, Jake, Victor M Vargas Barroso, and Peter M Jonas. “Cell-Specific
    Wiring Routes Information Flow through Hippocampal CA3.” <i>Cell Reports</i>.
    Elsevier, 2025. <a href="https://doi.org/10.1016/j.celrep.2025.116080">https://doi.org/10.1016/j.celrep.2025.116080</a>.
  ieee: J. Watson, V. M. Vargas Barroso, and P. M. Jonas, “Cell-specific wiring routes
    information flow through hippocampal CA3,” <i>Cell Reports</i>, vol. 44, no. 8.
    Elsevier, 2025.
  ista: Watson J, Vargas Barroso VM, Jonas PM. 2025. Cell-specific wiring routes information
    flow through hippocampal CA3. Cell Reports. 44(8), 116080.
  mla: Watson, Jake, et al. “Cell-Specific Wiring Routes Information Flow through
    Hippocampal CA3.” <i>Cell Reports</i>, vol. 44, no. 8, 116080, Elsevier, 2025,
    doi:<a href="https://doi.org/10.1016/j.celrep.2025.116080">10.1016/j.celrep.2025.116080</a>.
  short: J. Watson, V.M. Vargas Barroso, P.M. Jonas, Cell Reports 44 (2025).
corr_author: '1'
date_created: 2025-08-03T22:01:30Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2025-09-30T14:12:02Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1016/j.celrep.2025.116080
ec_funded: 1
external_id:
  isi:
  - '001544472300002'
file:
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  checksum: 556ff9760661ecd23949d75031043b1f
  content_type: application/pdf
  creator: dernst
  date_created: 2025-08-04T06:53:07Z
  date_updated: 2025-08-04T06:53:07Z
  file_id: '20106'
  file_name: 2025_CellReports_Watson.pdf
  file_size: 27695214
  relation: main_file
  success: 1
file_date_updated: 2025-08-04T06:53:07Z
has_accepted_license: '1'
intvolume: '        44'
isi: 1
issue: '8'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '692692'
  name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: fc2be41b-9c52-11eb-aca3-faa90aa144e9
  call_identifier: H2020
  grant_number: '101026635'
  name: Synaptic computations of the hippocampal CA3 circuitry
- _id: bd88be38-d553-11ed-ba76-81d5a70a6ef5
  grant_number: P36232
  name: Mechanisms of GABA release in hippocampal circuits
- _id: 260C2330-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '754411'
  name: ISTplus - Postdoctoral Fellowships
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell-specific wiring routes information flow through hippocampal CA3
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 44
year: '2025'
...