--- OA_place: publisher _id: '19048' abstract: - lang: eng text: 'Rotations are found in physics problems at all scales: from spatial motion of celestial bodies, to transitions between quantum states of atoms and molecules. Mathematically, they represent a fundamental class of transformations and symmetries. Unlike spatial displacements, rotational transformations in three-dimensional space are non-commutative: the result of applying a sequence of rotations depends on the order of these operations. This feature makes the emergent physics that involves rotations rather intricate, but instrumental for studies of highly-interconnected many-body systems. In the presence of an environment, rotational properties of an object change, due to the interaction with particles of the environment. Owing to the complexity of this interaction, it can be engineered to exhibit certain properties of interest. In this Thesis, we examine several scenarios of how the rotational behavior of an impurity can be modified by interactions with its environment.' acknowledged_ssus: - _id: CampIT - _id: E-Lib - _id: SSU acknowledgement: "I am grateful to the European Research Council (ERC) [10.3030/801770] and Austrian\r\nScience Fund (FWF) [10.55776/F1004] for funding my research and to the Physical\r\nReview journals for publishing it. I also want to thank the VCQ (previously CoQuS) and\r\nIQOQI for organizing wonderful networking events for the physics community in Vienna\r\nand Innsbruck, respectively. Moreover, I thank Austrian Science Fund (FWF) for the\r\ncontinuous support for quantum research." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Mikhail full_name: Maslov, Mikhail id: 2E65BB0E-F248-11E8-B48F-1D18A9856A87 last_name: Maslov orcid: 0000-0003-4074-2570 citation: ama: Maslov M. Emergent physics of rotating quantum impurities in many-body environments. 2025. doi:10.15479/at:ista:19048 apa: Maslov, M. (2025). Emergent physics of rotating quantum impurities in many-body environments. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:19048 chicago: Maslov, Mikhail. “Emergent Physics of Rotating Quantum Impurities in Many-Body Environments.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/at:ista:19048. ieee: M. Maslov, “Emergent physics of rotating quantum impurities in many-body environments,” Institute of Science and Technology Austria, 2025. ista: Maslov M. 2025. Emergent physics of rotating quantum impurities in many-body environments. Institute of Science and Technology Austria. mla: Maslov, Mikhail. Emergent Physics of Rotating Quantum Impurities in Many-Body Environments. Institute of Science and Technology Austria, 2025, doi:10.15479/at:ista:19048. short: M. Maslov, Emergent Physics of Rotating Quantum Impurities in Many-Body Environments, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-02-18T01:41:27Z date_published: 2025-02-18T00:00:00Z date_updated: 2026-04-16T12:20:38Z day: '18' ddc: - '539' - '535' - '541' degree_awarded: PhD department: - _id: GradSch - _id: MiLe doi: 10.15479/at:ista:19048 ec_funded: 1 file: - access_level: open_access checksum: 5822a4dd31724c512b37c658af1787ab content_type: application/pdf creator: mmaslov date_created: 2025-02-18T14:25:59Z date_updated: 2025-02-18T14:25:59Z file_id: '19061' file_name: thesis_Maslov.pdf file_size: 7779825 relation: main_file - access_level: open_access checksum: 89bdce4774406d26ceca88a8bbcd6a9a content_type: application/zip creator: mmaslov date_created: 2025-02-18T14:25:59Z date_updated: 2025-02-18T14:25:59Z file_id: '19062' file_name: thesis_Maslov_source.zip file_size: 14453726 relation: source_file file_date_updated: 2025-02-18T14:25:59Z has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by-nc-sa/4.0/ month: '02' oa: 1 oa_version: Published Version page: '86' project: - _id: 2688CF98-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '801770' name: 'Angulon: physics and applications of a new quasiparticle' - _id: 7c040762-9f16-11ee-852c-dd79eeee4ab3 grant_number: F100403 name: Coherent Optical Metrology Beyond Electric-Dipole-Allowed Transitions publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '10845' relation: part_of_dissertation status: public - id: '7933' relation: part_of_dissertation status: public - id: '18087' relation: part_of_dissertation status: public status: public supervisor: - first_name: Mikhail full_name: Lemeshko, Mikhail id: 37CB05FA-F248-11E8-B48F-1D18A9856A87 last_name: Lemeshko orcid: 0000-0002-6990-7802 title: Emergent physics of rotating quantum impurities in many-body environments tmp: image: /images/cc_by_nc_sa.png legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) short: CC BY-NC-SA (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '19557' acknowledged_ssus: - _id: Bio - _id: PreCl - _id: ScienComp acknowledgement: "The work presented in this doctoral thesis was performed at the Institute of Science\r\nand Technology (ISTA) and financially supported by a European Research Council\r\n(ERC) Consolidator Grant (PR1028ERC02), by SFARI (PR1028SIM02) and by the\r\nAustrian Science Fund (FWF) to Gaia Novarino (PE1028W1232). I am very thankful\r\nto the Doctoral Program “Molecular Drug Targets” (MolTag) for offering me financial\r\nsupport to perform essential experiments during my PhD studies and to participate in\r\ninternational conferences and courses." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Lena A full_name: Schwarz, Lena A id: 29A8453C-F248-11E8-B48F-1D18A9856A87 last_name: Schwarz citation: ama: Schwarz LA. Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution. 2025. doi:10.15479/AT-ISTA-19557 apa: Schwarz, L. A. (2025). Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19557 chicago: Schwarz, Lena A. “Mapping Developmental Dynamics of Autism Spectrum Disorder Mouse Models at Single-Cell Resolution.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19557. ieee: L. A. Schwarz, “Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution,” Institute of Science and Technology Austria, 2025. ista: Schwarz LA. 2025. Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution. Institute of Science and Technology Austria. mla: Schwarz, Lena A. Mapping Developmental Dynamics of Autism Spectrum Disorder Mouse Models at Single-Cell Resolution. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19557. short: L.A. Schwarz, Mapping Developmental Dynamics of Autism Spectrum Disorder Mouse Models at Single-Cell Resolution, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-04-14T06:59:06Z date_published: 2025-04-14T00:00:00Z date_updated: 2026-04-14T09:07:14Z day: '14' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: GaNo doi: 10.15479/AT-ISTA-19557 file: - access_level: closed checksum: 50290a8604edb0a720387f01e9d59fe4 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: lschwarz date_created: 2025-04-15T08:43:36Z date_updated: 2025-04-15T08:43:36Z file_id: '19561' file_name: Schwarz_Thesis_2025_FINAL.docx file_size: 21783427 relation: source_file - access_level: closed checksum: ed028488180ac4901e018ef1c330cf01 content_type: application/pdf creator: lschwarz date_created: 2025-04-15T08:43:42Z date_updated: 2026-03-27T13:15:08Z embargo: 2026-10-15 embargo_to: open_access file_id: '19562' file_name: Schwarz_Thesis_2025_FINALpdfa.pdf file_size: 11432175 relation: main_file file_date_updated: 2026-03-27T13:15:08Z has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by/4.0/ month: '04' oa_version: Published Version page: '124' project: - _id: 9B91375C-BA93-11EA-9121-9846C619BF3A grant_number: '707964' name: Critical windows and reversibility of ASD associated with mutations in chromatin remodelers - _id: 34ba8964-11ca-11ed-8bc3-e15864e7e9a6 grant_number: '101044865' name: Toward an understanding of the brain interstitial system and the extracellular proteome in health and autism spectrum disorders - _id: 2548AE96-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W1232 name: Molecular Drug Targets publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '12802' relation: part_of_dissertation status: public - id: '9429' relation: part_of_dissertation status: public status: public supervisor: - first_name: Gaia full_name: Novarino, Gaia id: 3E57A680-F248-11E8-B48F-1D18A9856A87 last_name: Novarino orcid: 0000-0002-7673-7178 title: Mapping developmental dynamics of autism spectrum disorder mouse models at single-cell resolution tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '19630' abstract: - lang: eng text: "This thesis consists of three chapters, each corresponding to one publication. While each of these projects tackles a topic in a different area of research, they all share a common thread in the type of topological structure they handle - a partition of space into volumes separated by interfaces that meet in non-manifold junctions.\r\n\r\nIn Chapter 2, we study clusters of soap bubbles from a simulation perspective. In particular, we develop a surface-only algorithm that couples large scale motion and shape deformation of soap bubble clusters with the small scale evolution of the thin film's thickness, which is responsible for visual phenomena like surface vortices, Newton's interference patterns, capillary waves, and deformation-dependent rupturing of films in a foam. We model film thickness as a reduced degree of freedom in the Navier-Stokes equations and from them derive three sets of equations governing normal and tangential motion of the soap film surface, as well as the evolution of the thin film thickness. We discretize these equations on a non-manifold triangle mesh, extending and adapting operators to handle complex topology. We also present an incompressible fluid solver for 2.5D films and an advection algorithm for convecting fields across non-manifold surface junctions. Our simulations enhance bubble solvers with additional effects caused by convection, rippling, draining, and evaporation of the thin film.\r\n\r\nIn Chapter 3, we introduce a multi-material non-manifold mesh-based surface tracking algorithm that converts mesh defects, such as overlaps, self-intersections, and inversions into topological changes. Our algorithm generalizes prior work on manifold surface tracking with topological changes: it preserves surface features like mesh-based methods, and it robustly handles topological changes like level set methods. Our method also offers improved efficiency and robustness over the state of the art. We demonstrate the effectiveness of the approach on a range of examples, including complex soap film simulations, such as those presented in Chapter 2, but with an order of magnitude more interacting bubbles than what we could achieve before, and Boolean unions of non-manifold meshes consisting of millions of triangles.\r\n\r\nLastly, in Chapter 4, we utilize developments in the theory of random geometric complexes facilitated by observations from Discrete Morse theory. We survey the methods and results obtained with this new approach, and discuss some of its shortcomings. We use simulations to illustrate the results and to form conjectures, getting numerical estimates for combinatorial, topological, and geometric properties of weighted and unweighted Delaunay mosaics, their dual Voronoi tessellations, and the Alpha and Wrap complexes contained in the mosaics." acknowledged_ssus: - _id: ScienComp acknowledgement: The project in Chapter 2 has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme under grant agreement No. 638176. The project in Chapter 3 was funded in part by the European Union (ERC-2021-COG 101045083 CoDiNA). The project in Chapter 4 has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreements No 78818 Alpha and No 638176). It was also partially supported by the DFG Collaborative Research Center TRR 109, 'Discretization in Geometry and Dynamics', through grant no. I02979-N35 of the Austrian Science Fund (FWF). Thank you for providing funds to support my work. alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Peter full_name: Synak, Peter id: 331776E2-F248-11E8-B48F-1D18A9856A87 last_name: Synak citation: ama: Synak P. Methods for fluid simulation, surface tracking, and statistics of non-manifold structures. 2025. doi:10.15479/AT-ISTA-19630 apa: Synak, P. (2025). Methods for fluid simulation, surface tracking, and statistics of non-manifold structures. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19630 chicago: Synak, Peter. “Methods for Fluid Simulation, Surface Tracking, and Statistics of Non-Manifold Structures.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19630. ieee: P. Synak, “Methods for fluid simulation, surface tracking, and statistics of non-manifold structures,” Institute of Science and Technology Austria, 2025. ista: Synak P. 2025. Methods for fluid simulation, surface tracking, and statistics of non-manifold structures. Institute of Science and Technology Austria. mla: Synak, Peter. Methods for Fluid Simulation, Surface Tracking, and Statistics of Non-Manifold Structures. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19630. short: P. Synak, Methods for Fluid Simulation, Surface Tracking, and Statistics of Non-Manifold Structures, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-04-29T09:39:34Z date_published: 2025-04-29T00:00:00Z date_updated: 2026-04-16T08:29:34Z day: '29' ddc: - '519' - '006' degree_awarded: PhD department: - _id: ChWo - _id: GradSch doi: 10.15479/AT-ISTA-19630 ec_funded: 1 file: - access_level: closed checksum: f00b519c27529daa0c3b2d4102b4fa7b content_type: application/x-zip-compressed creator: cchlebak date_created: 2025-04-30T14:02:25Z date_updated: 2025-04-30T14:02:25Z file_id: '19633' file_name: Thesis_source_Heiss_Synak.zip file_size: 60670543 relation: source_file - access_level: open_access checksum: 6e40a2fd3b1b881af1385670854a682e content_type: application/pdf creator: cchlebak date_created: 2025-04-30T14:02:42Z date_updated: 2025-04-30T15:49:16Z file_id: '19634' file_name: Thesis_PDFA_Heiss_Synak.pdf file_size: 21319043 relation: main_file file_date_updated: 2025-04-30T15:49:16Z has_accepted_license: '1' language: - iso: eng month: '04' oa: 1 oa_version: Published Version page: '106' project: - _id: 2533E772-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '638176' name: 'Big Splash: Efficient Simulation of Natural Phenomena at Extremely Large Scales' - _id: 34bc2376-11ca-11ed-8bc3-9a3b3961a088 grant_number: '101045083' name: Computational Discovery of Numerical Algorithms for Animation and Simulation of Natural Phenomena - _id: 266A2E9E-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '788183' name: Alpha Shape Theory Extended - _id: 2533E772-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '638176' name: 'Big Splash: Efficient Simulation of Natural Phenomena at Extremely Large Scales' - _id: 2561EBF4-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: I02979-N35 name: Persistence and stability of geometric complexes publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '8135' relation: part_of_dissertation status: public - id: '17219' relation: part_of_dissertation status: public - id: '8384' relation: part_of_dissertation status: public status: public supervisor: - first_name: Christopher J full_name: Wojtan, Christopher J id: 3C61F1D2-F248-11E8-B48F-1D18A9856A87 last_name: Wojtan orcid: 0000-0001-6646-5546 title: Methods for fluid simulation, surface tracking, and statistics of non-manifold structures type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '19903' abstract: - lang: eng text: "Cooperation, that is, one person paying a cost for another's benefit, is a fundamental principle without which no form of society could exist. The extent to which humans cooperate with each other is also an essential feature that differentiates them from other animals. Cooperation occurs even in the absence of altruistic motivations, when it is selfishly incentivised by the expectation of a future reward. For example, many economic interactions are well described that way. This kind of cooperation requires that people exhibit reciprocal behaviour that acts as a mechanism that rewards cooperation.\r\nWith game-theoretic models, it is possible to formally study potential such mechanisms and under what conditions they can exist. This thesis contributes to this effort by analysing recently introduced models of cooperation that advance on previous work by taking into account the potential for pre-existing inequality among cooperating individuals as well as the different forms that reciprocity can take.\r\nIndividuals may differ both intrinsically, in their abilities, as well as extrinsically, in the amount of resources they have available. Allowing for such differences in a model of cooperation helps to understand how inequality affects the potential for, and outcomes of, cooperation among unequals. In this thesis, it is shown that in the presence of intrinsic inequality, a similar unequal distribution of resources can increase the potential for cooperation. This effect is stronger the smaller the group is in which cooperation takes place. It is also shown that under particular assumptions, if the unequal members of a group vary the size of their contributions to a cooperative effort over time, they can thereby increase their efficiency and improve the collective outcome.\r\nCooperative behaviour in a two-person interaction can be rewarded either by direct reciprocation whenever the same two people interact again, or indirectly by a third party who observed the interaction. In the latter case of indirect reciprocity, individuals are proximally rewarded by a good reputation, which ultimately translates to being rewarded with cooperative behaviour by others. This mechanism can enable selfishly motivated cooperation even in circumstances where individuals are unlikely to meet again, akin to how money facilitates trade. While these two forms of reciprocity have mostly been studied in isolation, this thesis analyses both direct and indirect reciprocity in a general model in order to compare their relative effectiveness under different circumstances. The contribution of this thesis is an extension of previous work regarding a specific kind of interaction, whose parameters allow for convenient mathematical analysis, to the most general set of possible interactions." acknowledgement: "The research for this thesis was supported by the European Research Council\r\n(grant agreements No. 863818 and No. 850529), the European Union’s Horizon 2020 research and innovation programme (Marie Skłodowska-Curie grant agreement No. 754411),\r\nthe Austrian Science Fund (grant DOI 10.55776/COE12), the French Agence Nationale\r\nde la Recherche under the Programme d’investissements d’avenir (project reference 17-\r\nEURE-0010) and the Australian Government through the Australian Research Council\r\n(grant No. SR200100005, “Securing Antarctica’s Environmental Future”)." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Valentin full_name: Hübner, Valentin id: 2c8aa207-dc7d-11ea-9b2f-f22972ecd910 last_name: Hübner orcid: 0009-0001-5009-4987 citation: ama: Hübner V. Reciprocity and inequality in social dilemmas. 2025. doi:10.15479/AT-ISTA-19903 apa: Hübner, V. (2025). Reciprocity and inequality in social dilemmas. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19903 chicago: Hübner, Valentin. “Reciprocity and Inequality in Social Dilemmas.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19903. ieee: V. Hübner, “Reciprocity and inequality in social dilemmas,” Institute of Science and Technology Austria, 2025. ista: Hübner V. 2025. Reciprocity and inequality in social dilemmas. Institute of Science and Technology Austria. mla: Hübner, Valentin. Reciprocity and Inequality in Social Dilemmas. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19903. short: V. Hübner, Reciprocity and Inequality in Social Dilemmas, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-06-25T13:50:10Z date_published: 2025-06-25T00:00:00Z date_updated: 2026-04-07T12:30:57Z day: '25' ddc: - '519' degree_awarded: PhD department: - _id: GradSch - _id: KrCh doi: 10.15479/AT-ISTA-19903 ec_funded: 1 file: - access_level: closed checksum: 794c02f8c82ca59ba6dda3bd7eed871a content_type: application/x-xz creator: vhuebner date_created: 2025-06-25T13:38:07Z date_updated: 2025-06-25T13:38:07Z file_id: '19905' file_name: Thesis Valentin Hübner source.tar.xz file_size: 6192760 relation: source_file - access_level: open_access checksum: ac56063d81c81e40322b6ff5a8c4912e content_type: application/pdf creator: vhuebner date_created: 2025-07-09T13:37:00Z date_updated: 2025-07-09T13:37:00Z file_id: '19976' file_name: Thesis Valentin Hübner.pdf file_size: 4837864 relation: main_file file_date_updated: 2025-07-09T13:37:00Z has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by-nc/4.0/ month: '06' oa: 1 oa_version: Published Version page: '157' project: - _id: 0599E47C-7A3F-11EA-A408-12923DDC885E call_identifier: H2020 grant_number: '863818' name: 'Formal Methods for Stochastic Models: Algorithms and Applications' - _id: 260C2330-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '754411' name: ISTplus - Postdoctoral Fellowships publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '19843' relation: part_of_dissertation status: public - id: '15083' relation: part_of_dissertation status: public - id: '19074' relation: part_of_dissertation status: public status: public supervisor: - first_name: Krishnendu full_name: Chatterjee, Krishnendu id: 2E5DCA20-F248-11E8-B48F-1D18A9856A87 last_name: Chatterjee orcid: 0000-0002-4561-241X title: Reciprocity and inequality in social dilemmas tmp: image: /images/cc_by_nc.png legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) short: CC BY-NC (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20149' abstract: - lang: eng text: "Immune responses depend on the coordinated and efficient migration of leukocytes. These\r\ncells, which are embedded and tightly confined within tissues, must navigate and traverse\r\ndiverse and complex three-dimensional environments. Leukocytes adapt their locomotory\r\nbehavior to the mechanical, geometrical, and biochemical characteristics of their\r\nsurroundings. In low-density environments, where the pore size of the interstitial matrix\r\nallows free passage, these cells position the nucleus directly behind the lamellipodium, the\r\nprotrusive actin structure that forms the leading front of the cell. In this configuration, they\r\nuse the nucleus as a gauge to identify the path of least resistance.\r\nHere, we show that in high-density environments, where the pore size precludes free passage\r\nof the cell body, leukocytes reposition the microtubule-organizing center (MTOC) and\r\nassociated organelles in front of the nucleus. In this configuration, they use actin structures\r\nprotruding orthogonally to the direction of migration in order to open a path for the cell body.\r\nWe identify two distinct actin populations that serve this purpose at different subcellular\r\nlocalizations. At the leading edge, local indentation of the plasma membrane leads to\r\nrecruitment of the Wiskott-Aldrich syndrome protein (WASp), which, via Arp2/3, results in\r\nthe formation of individual actin foci. At the cell body, actin polymerization is triggered by\r\nDOCK8, a Cdc42 exchange factor, resulting in the formation of a central actin pool.\r\nWe demonstrate that the central and peripheral actin pools are functionally communicating\r\nand that depletion of the central actin pool leads to increased actin accumulation at the cell\r\nfront, resulting in excessive extension of the leading edge." acknowledged_ssus: - _id: Bio - _id: LifeSc - _id: M-Shop - _id: NanoFab acknowledgement: "I would like to acknowledge the\r\nfinancial support of the European Research Council through the ERC-SyG grant “Pushing from\r\nwithin: Control of cell shape, integrity and motility by cytoskeletal pushing forces”\r\n(01071793), which made this research possible. " alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Patricia full_name: Dos Reis Rodrigues, Patricia id: 26E95904-5160-11E9-9C0B-C5B0DC97E90F last_name: Dos Reis Rodrigues orcid: 0000-0003-1681-508X citation: ama: Dos Reis Rodrigues P. Coordination of protrusive forces in immune cell migration . 2025. doi:10.15479/AT-ISTA-20149 apa: Dos Reis Rodrigues, P. (2025). Coordination of protrusive forces in immune cell migration . Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20149 chicago: Dos Reis Rodrigues, Patricia. “Coordination of Protrusive Forces in Immune Cell Migration .” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20149. ieee: P. Dos Reis Rodrigues, “Coordination of protrusive forces in immune cell migration ,” Institute of Science and Technology Austria, 2025. ista: Dos Reis Rodrigues P. 2025. Coordination of protrusive forces in immune cell migration . Institute of Science and Technology Austria. mla: Dos Reis Rodrigues, Patricia. Coordination of Protrusive Forces in Immune Cell Migration . Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20149. short: P. Dos Reis Rodrigues, Coordination of Protrusive Forces in Immune Cell Migration , Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-08-08T09:18:02Z date_published: 2025-08-08T00:00:00Z date_updated: 2026-04-28T13:26:50Z day: '08' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: MiSi doi: 10.15479/AT-ISTA-20149 file: - access_level: open_access checksum: fda8a1070667c3562263f4867609b41b content_type: application/pdf creator: prodrigu date_created: 2025-08-27T12:59:10Z date_updated: 2025-08-27T12:59:10Z file_id: '20232' file_name: 2025_ReisRodrigues_Patricia_Thesis.pdf file_size: 63885565 relation: main_file success: 1 - access_level: closed checksum: e8b65affcbce846a926454df4b2867b9 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: prodrigu date_created: 2025-08-27T13:00:30Z date_updated: 2025-08-27T13:02:28Z file_id: '20233' file_name: 2025_ReisRodrigues_Patricia_Thesis.docx file_size: 50483434 relation: source_file file_date_updated: 2025-08-27T13:02:28Z has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by-nc-nd/4.0/ month: '08' oa: 1 oa_version: Published Version page: '114' project: - _id: bd91e723-d553-11ed-ba76-fe7eeb2185fd grant_number: '101071793' name: 'Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '10703' relation: part_of_dissertation status: public - id: '20082' relation: part_of_dissertation status: public status: public supervisor: - first_name: Michael K full_name: Sixt, Michael K id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87 last_name: Sixt orcid: 0000-0002-6620-9179 title: 'Coordination of protrusive forces in immune cell migration ' tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20206' abstract: - lang: eng text: "The internal structure of biomolecules and their organization in higher-order arrangements are key factors governing the working principles of biological systems. Bioimaging has successfully revealed arrangements across relevant spatial scales. For example, cryo-electron tomography has become widely used for analyzing biomolecular structures in situ due to its comprehensive structural visualization of near-natively preserved samples, and its capability of sub-nm resolution via averaging. However, the identification of molecules within crowded cellular environments is often hindered by low contrast. Fluorescence microscopy, on the other hand, routinely visualizes specifically labeled targets at single-molecule contrast against essentially zero background. Moreover, it provides comparatively high throughput and is amenable to multiplexing. Due to this complementarity, combining datasets from both modalities acquired on the same region via correlative light and electron microscopy can reveal novel types of information. \r\nThe spatial scale at which information can be extracted depends on imaging resolution and correlation accuracy. Since diffraction of light limits the resolution of conventional fluorescence microscopy to few hundreds of nanometers, reaching the full potential of correlative imaging requires super-resolution approaches. Performing imaging at cryogenic temperature preserves structures in a near-native state and minimizes distortions between the fluorescence and the electron microscopy datasets. Implementations of this concept have achieved correlation on the scale of cellular organelles or bacterial domains.\r\nWe have worked towards pushing correlative imaging to the single-molecule scale by improving cryo-super-resolution microscopy, and devising a refined image correlation workflow. As part of this project, I constructed a microscopy setup and adopted it for super-resolution fluorescence microscopy at room temperature and cryogenic conditions. I explored different cryo-stages and acquisition strategies. Specifically, I developed a new scheme for correcting sample drift, thus increasing mechanical stability during microscopy acquisitions.\r\n" acknowledged_ssus: - _id: M-Shop - _id: EM-Fac - _id: Bio acknowledgement: "The project was supported by CZI grant DAF2021-234754 and grant\r\nDOI: https://doi.org/10.37921/812628ebpcwg from the Chan Zuckerberg Initiative DAF, an\r\nadvised fund of Silicon Valley Community Foundation (funder\r\nDOI: https://doi.org/10.13039/100014989), as well as internal grants from ISTA’s Equipment\r\nInvestment Committee and Interdisciplinary Project Committee. " alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Jakob full_name: Vorlaufer, Jakob id: 937696FA-C996-11E9-8C7C-CF13E6697425 last_name: Vorlaufer orcid: 0009-0000-7590-3501 citation: ama: Vorlaufer J. Construction of a cryo-super-resolution microscope to guide in situ structure analysis. 2025. doi:10.15479/AT-ISTA-20206 apa: Vorlaufer, J. (2025). Construction of a cryo-super-resolution microscope to guide in situ structure analysis. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20206 chicago: Vorlaufer, Jakob. “Construction of a Cryo-Super-Resolution Microscope to Guide in Situ Structure Analysis.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20206. ieee: J. Vorlaufer, “Construction of a cryo-super-resolution microscope to guide in situ structure analysis,” Institute of Science and Technology Austria, 2025. ista: Vorlaufer J. 2025. Construction of a cryo-super-resolution microscope to guide in situ structure analysis. Institute of Science and Technology Austria. mla: Vorlaufer, Jakob. Construction of a Cryo-Super-Resolution Microscope to Guide in Situ Structure Analysis. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20206. short: J. Vorlaufer, Construction of a Cryo-Super-Resolution Microscope to Guide in Situ Structure Analysis, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-08-22T08:12:55Z date_published: 2025-08-25T00:00:00Z date_updated: 2026-04-07T11:48:07Z day: '25' ddc: - '621' - '535' degree_awarded: PhD department: - _id: GradSch - _id: JoDa doi: 10.15479/AT-ISTA-20206 file: - access_level: closed checksum: 191db3367c19c9b32b65f4bc3a7c19de content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: jvorlauf date_created: 2025-08-25T13:49:55Z date_updated: 2025-08-25T13:49:55Z file_id: '20228' file_name: 2025_Vorlaufer_Jakob_Thesis.docx file_size: 39735535 relation: source_file - access_level: open_access checksum: 104400e6036921569610230c1d4899dc content_type: application/pdf creator: jvorlauf date_created: 2025-08-25T13:49:56Z date_updated: 2025-08-25T13:49:56Z file_id: '20229' file_name: 2025_Vorlaufer_Jakob_Thesis.pdf file_size: 10947446 relation: main_file success: 1 file_date_updated: 2025-08-25T13:49:56Z has_accepted_license: '1' language: - iso: eng month: '08' oa: 1 oa_version: Published Version page: '107' project: - _id: 62909c6f-2b32-11ec-9570-e1476aab5308 grant_number: CZI01 name: CryoMinflux-guided in-situ molecular census and structure determination publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '19795' relation: part_of_dissertation status: public status: public supervisor: - first_name: Johann G full_name: Danzl, Johann G id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87 last_name: Danzl orcid: 0000-0001-8559-3973 title: Construction of a cryo-super-resolution microscope to guide in situ structure analysis tmp: image: /images/cc_by_nc_sa.png legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) short: CC BY-NC-SA (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20362' acknowledged_ssus: - _id: Bio - _id: LifeSc alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: David full_name: Babic, David id: db566d23-f6e0-11ea-865d-e6f270e968e7 last_name: Babic citation: ama: Babic D. Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana. 2025. doi:10.15479/AT-ISTA-20362 apa: Babic, D. (2025). Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20362 chicago: Babic, David. “Mechanisms of Auxin-Mediated Early Embryogenesis in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20362. ieee: D. Babic, “Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2025. ista: Babic D. 2025. Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana. Institute of Science and Technology Austria. mla: Babic, David. Mechanisms of Auxin-Mediated Early Embryogenesis in Arabidopsis Thaliana. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20362. short: D. Babic, Mechanisms of Auxin-Mediated Early Embryogenesis in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-09-17T13:28:01Z date_published: 2025-09-18T00:00:00Z date_updated: 2026-04-07T11:52:02Z day: '18' ddc: - '580' degree_awarded: PhD department: - _id: GradSch - _id: JiFr - _id: EvBe doi: 10.15479/AT-ISTA-20362 file: - access_level: closed checksum: 5ecf274281a54a41e0288bc79edf7492 content_type: application/pdf creator: dbabic date_created: 2025-09-24T13:43:14Z date_updated: 2025-09-26T07:29:11Z embargo: 2026-09-25 embargo_to: open_access file_id: '20388' file_name: 2025_David_Babic_Thesis.pdf file_size: 7501548 relation: main_file - access_level: closed checksum: 2703e548390de0a1af7a707137e8ab3b content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: dbabic date_created: 2025-09-24T13:43:14Z date_updated: 2025-09-26T07:29:11Z file_id: '20389' file_name: Thesis_Babic_draft.docx file_size: 23206052 relation: source_file file_date_updated: 2025-09-26T07:29:11Z has_accepted_license: '1' language: - iso: eng month: '09' oa_version: Published Version page: '116' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '20187' relation: part_of_dissertation status: public status: public supervisor: - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Eva full_name: Benková, Eva id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 title: Mechanisms of auxin-mediated early embryogenesis in Arabidopsis thaliana tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20393' acknowledged_ssus: - _id: Bio - _id: PreCl - _id: LifeSc alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Kasumi full_name: Kishi, Kasumi id: 3065DFC4-F248-11E8-B48F-1D18A9856A87 last_name: Kishi orcid: 0000-0001-6060-4795 citation: ama: Kishi K. Regulation of notochord and floor plate size during mouse development. 2025. doi:10.15479/AT-ISTA-20393 apa: Kishi, K. (2025). Regulation of notochord and floor plate size during mouse development. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20393 chicago: Kishi, Kasumi. “Regulation of Notochord and Floor Plate Size during Mouse Development.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20393. ieee: K. Kishi, “Regulation of notochord and floor plate size during mouse development,” Institute of Science and Technology Austria, 2025. ista: Kishi K. 2025. Regulation of notochord and floor plate size during mouse development. Institute of Science and Technology Austria. mla: Kishi, Kasumi. Regulation of Notochord and Floor Plate Size during Mouse Development. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20393. short: K. Kishi, Regulation of Notochord and Floor Plate Size during Mouse Development, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-09-25T10:08:10Z date_published: 2025-09-24T00:00:00Z date_updated: 2026-04-14T09:50:52Z day: '24' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: AnKi - _id: EdHa doi: 10.15479/AT-ISTA-20393 file: - access_level: closed checksum: 6bb5a7ce318dc3f7bd165f2523e77b89 content_type: application/x-zip-compressed creator: kkishi date_created: 2025-09-30T14:33:17Z date_updated: 2025-10-01T11:54:41Z file_id: '20413' file_name: 2025-Kishi-Kasumi-Thesis.zip file_size: 41847994 relation: source_file - access_level: closed checksum: 88349b9177e1dcbe1242cd3884b36fdb content_type: application/pdf creator: kkishi date_created: 2025-09-30T14:33:22Z date_updated: 2025-10-02T07:51:21Z embargo: 2026-09-30 embargo_to: open_access file_id: '20414' file_name: 2025-Kishi-Kasumi-Thesis.pdf file_size: 55747072 relation: main_file file_date_updated: 2025-10-02T07:51:21Z has_accepted_license: '1' language: - iso: eng month: '09' oa_version: Published Version page: '102' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '18481' relation: part_of_dissertation status: public status: public supervisor: - first_name: Anna full_name: Kicheva, Anna id: 3959A2A0-F248-11E8-B48F-1D18A9856A87 last_name: Kicheva orcid: 0000-0003-4509-4998 - first_name: Edouard B full_name: Hannezo, Edouard B id: 3A9DB764-F248-11E8-B48F-1D18A9856A87 last_name: Hannezo orcid: 0000-0001-6005-1561 title: Regulation of notochord and floor plate size during mouse development tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20415' acknowledged_ssus: - _id: LifeSc - _id: EM-Fac alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Seungho full_name: Lee, Seungho id: BB243B88-D767-11E9-B658-BC13E6697425 last_name: Lee orcid: 0000-0002-6962-8598 citation: ama: Lee S. Nanoparticle-based precursors toward advanced crystalline inorganic solids. 2025. doi:10.15479/AT-ISTA-20415 apa: Lee, S. (2025). Nanoparticle-based precursors toward advanced crystalline inorganic solids. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20415 chicago: Lee, Seungho. “Nanoparticle-Based Precursors toward Advanced Crystalline Inorganic Solids.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20415. ieee: S. Lee, “Nanoparticle-based precursors toward advanced crystalline inorganic solids,” Institute of Science and Technology Austria, 2025. ista: Lee S. 2025. Nanoparticle-based precursors toward advanced crystalline inorganic solids. Institute of Science and Technology Austria. mla: Lee, Seungho. Nanoparticle-Based Precursors toward Advanced Crystalline Inorganic Solids. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20415. short: S. Lee, Nanoparticle-Based Precursors toward Advanced Crystalline Inorganic Solids, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-01T09:04:00Z date_published: 2025-10-01T00:00:00Z date_updated: 2026-04-07T11:52:32Z day: '01' ddc: - '540' degree_awarded: PhD department: - _id: GradSch - _id: MaIb doi: 10.15479/AT-ISTA-20415 file: - access_level: closed checksum: fa6d5946feb37b678ee1c6dffb4fa167 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: slee date_created: 2025-10-03T12:29:43Z date_updated: 2025-10-07T08:57:14Z file_id: '20420' file_name: 2025_Lee_Seungho_Thesis.docx file_size: 88706648 relation: source_file - access_level: closed checksum: c5ba6d464113ad0c5812a9d24b539b86 content_type: application/pdf creator: slee date_created: 2025-10-03T12:29:25Z date_updated: 2025-10-03T12:29:25Z embargo: 2026-10-03 embargo_to: open_access file_id: '20421' file_name: 2025_Lee_Seungho_Thesis__.pdf file_size: 14587276 relation: main_file file_date_updated: 2025-10-07T08:57:14Z has_accepted_license: '1' language: - iso: eng month: '10' oa_version: Published Version page: '144' project: - _id: 9B8F7476-BA93-11EA-9121-9846C619BF3A name: 'HighTE: The Werner Siemens Laboratory for the High Throughput Discovery of Semiconductors for Waste Heat Recovery' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '15357' relation: part_of_dissertation status: public - id: '12237' relation: part_of_dissertation status: public status: public supervisor: - first_name: Maria full_name: Ibáñez, Maria id: 43C61214-F248-11E8-B48F-1D18A9856A87 last_name: Ibáñez orcid: 0000-0001-5013-2843 - first_name: Loredana full_name: Protesescu, Loredana last_name: Protesescu - first_name: Stefan Alexander full_name: Freunberger, Stefan Alexander id: A8CA28E6-CE23-11E9-AD2D-EC27E6697425 last_name: Freunberger orcid: 0000-0003-2902-5319 title: Nanoparticle-based precursors toward advanced crystalline inorganic solids type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20467' acknowledged_ssus: - _id: Bio - _id: SSU - _id: PreCl - _id: LifeSc acknowledgement: "The work presented in this Thesis was carried out at the Institute of Science and Technology\r\nAustria (ISTA), and was supported by the Austrian Science Fund (FWF) [10.55776/P37131].\r\nI would like to thank the Scientific Service Units (SSU) of ISTA for the provided resources,\r\nspecifically the Imaging and Optics Facility (IOF), the Lab Support Facility (LSF), and the\r\nPre-Clinical Facility (PCF) team, specifically Sonja Haslinger, Claudia Gold, and Michael\r\nSchunn, for mouse colony management and support. " alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Florianne E full_name: Miteva, Florianne E id: 3526230C-F248-11E8-B48F-1D18A9856A87 last_name: Miteva citation: ama: Miteva FE. The role of cyclooxygenase 1 on microglial response to inflammatory stressors. 2025. doi:10.15479/AT-ISTA-20467 apa: Miteva, F. E. (2025). The role of cyclooxygenase 1 on microglial response to inflammatory stressors. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20467 chicago: Miteva, Florianne E. “The Role of Cyclooxygenase 1 on Microglial Response to Inflammatory Stressors.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20467. ieee: F. E. Miteva, “The role of cyclooxygenase 1 on microglial response to inflammatory stressors,” Institute of Science and Technology Austria, 2025. ista: Miteva FE. 2025. The role of cyclooxygenase 1 on microglial response to inflammatory stressors. Institute of Science and Technology Austria. mla: Miteva, Florianne E. The Role of Cyclooxygenase 1 on Microglial Response to Inflammatory Stressors. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20467. short: F.E. Miteva, The Role of Cyclooxygenase 1 on Microglial Response to Inflammatory Stressors, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-14T10:24:41Z date_published: 2025-10-14T00:00:00Z date_updated: 2026-05-20T06:37:12Z day: '14' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: SaSi doi: 10.15479/AT-ISTA-20467 file: - access_level: closed checksum: 03537697be8c688d3a05cf948288e48f content_type: application/pdf creator: fschootu date_created: 2025-10-17T11:09:11Z date_updated: 2025-10-17T11:13:25Z embargo: 2026-10-14 embargo_to: open_access file_id: '20484' file_name: 2025_Miteva_Florianne_thesis.pdf file_size: 13668588 relation: main_file - access_level: closed checksum: df4930d7211cf9cfe1254b77204dc1d3 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: fschootu date_created: 2025-10-23T11:33:06Z date_updated: 2025-10-23T11:33:06Z file_id: '20525' file_name: 2025_Miteva_florianne_thesis.docx file_size: 28991918 relation: source_file file_date_updated: 2025-10-23T11:33:06Z has_accepted_license: '1' language: - iso: eng month: '10' oa_version: Published Version page: '99' project: - _id: 7be82147-9f16-11ee-852c-f44682d73140 grant_number: P37131 name: Dissecting the morpho-functional relationship of microglia publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '19566' relation: part_of_dissertation status: public status: public supervisor: - first_name: Sandra full_name: Siegert, Sandra id: 36ACD32E-F248-11E8-B48F-1D18A9856A87 last_name: Siegert orcid: 0000-0001-8635-0877 title: The role of cyclooxygenase 1 on microglial response to inflammatory stressors tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20485' acknowledged_ssus: - _id: LifeSc - _id: Bio acknowledgement: "I would also like to acknowledge the funding that I received from the European Union’s\r\nHorizon 2020 research and Innovation programme under the Marie Sklodowska-Curie\r\nGrant Agreement No. 665385. This work would not have been possible without the contribution and support of people\r\nbehind the scientific service units at ISTA: the Life Science Facility (LSF), Imaging and\r\nOptics Facility (IOF), the Bioinformatics Unit, Protein Services Unit and\r\nElectrophysiology Unit. I would also like to recognize the work of people at the Vienna\r\nBiocenter (VBC) Mass Spectrometry Facility, particularly Markus Hartl and WeiQiang\r\nChen. " alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Michaela full_name: Misova, Michaela id: 495A3C32-F248-11E8-B48F-1D18A9856A87 last_name: Misova orcid: 0000-0003-2427-6856 citation: ama: Misova M. Dissecting gap junction biology using the C. elegans nervous system. 2025. doi:10.15479/AT-ISTA-20485 apa: Misova, M. (2025). Dissecting gap junction biology using the C. elegans nervous system. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20485 chicago: Misova, Michaela. “Dissecting Gap Junction Biology Using the C. Elegans Nervous System.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20485. ieee: M. Misova, “Dissecting gap junction biology using the C. elegans nervous system,” Institute of Science and Technology Austria, 2025. ista: Misova M. 2025. Dissecting gap junction biology using the C. elegans nervous system. Institute of Science and Technology Austria. mla: Misova, Michaela. Dissecting Gap Junction Biology Using the C. Elegans Nervous System. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20485. short: M. Misova, Dissecting Gap Junction Biology Using the C. Elegans Nervous System, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-17T16:15:09Z date_published: 2025-10-23T00:00:00Z date_updated: 2026-04-07T11:54:00Z day: '23' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: MaDe doi: 10.15479/AT-ISTA-20485 ec_funded: 1 file: - access_level: closed checksum: e042ea314e7e13fce76c6c95e126779a content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: mmisova date_created: 2025-10-23T08:22:35Z date_updated: 2025-11-06T11:08:06Z file_id: '20518' file_name: 2025-Misova-Michaela-Thesis.docx file_size: 75070995 relation: source_file - access_level: closed checksum: fcd8973d6a025256eb0eb1a82c02172c content_type: application/pdf creator: mmisova date_created: 2025-10-23T08:21:21Z date_updated: 2025-10-23T08:21:21Z embargo: 2026-10-23 embargo_to: open_access file_id: '20519' file_name: 2025-Misova-Michaela-Thesis.pdf file_size: 10974630 relation: main_file file_date_updated: 2025-11-06T11:08:06Z has_accepted_license: '1' language: - iso: eng month: '10' oa_version: Published Version page: '155' project: - _id: 2564DBCA-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '665385' name: International IST Doctoral Program publication_identifier: isbn: - 978-3-99078-068-8 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria status: public supervisor: - first_name: Mario full_name: de Bono, Mario id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87 last_name: de Bono orcid: 0000-0001-8347-0443 title: Dissecting gap junction biology using the C. elegans nervous system tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20551' abstract: - lang: eng text: "The space of codimension-2 shapes, such as curves in 3D and surfaces in 4D, is an infinite-dimensional manifold. This thesis explores geometric structures and dynamics on this space, with emphasis on their implications for physics, particularly hydrodynamics.\r\n\r\nOur investigation ranges from theoretical studies of infinite-dimensional symplectic and prequantum geometry to numerical computation of the time evolution of shapes. The thesis presents four main contributions.\r\n\r\nIn the first part, we introduce implicit representations of codimension-2 shapes using a class of complex-valued functions, and prove that the space of these implicit representations forms a prequantum bundle over the codimension-2 shape space. This reveals a new geometric interpretation of the canonical symplectic structure on the codimension-2 shape space.\r\n\r\nIn the second part, we use implicit representations to develop a simulation method for the dynamics of space curves. To handle chaotic systems such as vortex filaments in hydrodynamics, we exploit the infinite degrees of freedom, hidden in both the configuration and dynamics of implicit representations.\r\n\r\nIn the third part, we introduce new symplectic structures on the space of space curves, which generalize the only previously known symplectic structure on this space, allowing for new Hamiltonian dynamics of space curves.\r\n\r\nIn the fourth part, we apply a symplectic viewpoint to a differential geometric problem with practical applications. We derive a new area formula for spherical polygons via prequantization. " acknowledged_ssus: - _id: CampIT acknowledgement: "Projects contained in this thesis were financially supported in part by the\r\nEuropean Research Council with grants 1. ERC Consolidator Grant 101045083 CoDiNA,\r\nand 2. the European Union’s Horizon 2020 research and innovation programme under grant\r\nagreement No. 638176." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Sadashige full_name: Ishida, Sadashige id: 6F7C4B96-A8E9-11E9-A7CA-09ECE5697425 last_name: Ishida orcid: 0000-0002-3121-3100 citation: ama: Ishida S. Symplectic-prequantum structures and dynamics on the codimension-2 shape space. 2025. doi:10.15479/AT-ISTA-20551 apa: Ishida, S. (2025). Symplectic-prequantum structures and dynamics on the codimension-2 shape space. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20551 chicago: Ishida, Sadashige. “Symplectic-Prequantum Structures and Dynamics on the Codimension-2 Shape Space.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20551. ieee: S. Ishida, “Symplectic-prequantum structures and dynamics on the codimension-2 shape space,” Institute of Science and Technology Austria, 2025. ista: Ishida S. 2025. Symplectic-prequantum structures and dynamics on the codimension-2 shape space. Institute of Science and Technology Austria. mla: Ishida, Sadashige. Symplectic-Prequantum Structures and Dynamics on the Codimension-2 Shape Space. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20551. short: S. Ishida, Symplectic-Prequantum Structures and Dynamics on the Codimension-2 Shape Space, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-27T10:28:52Z date_published: 2025-10-31T00:00:00Z date_updated: 2026-04-07T12:02:23Z day: '31' ddc: - '516' degree_awarded: PhD department: - _id: GradSch - _id: ChWo doi: 10.15479/AT-ISTA-20551 ec_funded: 1 file: - access_level: open_access checksum: 4eef80afcb67691cbb6549c4756fa534 content_type: application/zip creator: sishida date_created: 2025-11-01T18:26:14Z date_updated: 2025-11-01T18:26:14Z file_id: '20583' file_name: Thesis_tex.zip file_size: 72487812 relation: source_file - access_level: open_access checksum: 1e5a557900bf2dce01966b211b15d0fe content_type: application/pdf creator: sishida date_created: 2025-11-10T08:45:05Z date_updated: 2025-11-10T08:45:05Z file_id: '20623' file_name: Thesis_Sadashige_Ishida_PDFA.pdf file_size: 8945141 relation: main_file success: 1 file_date_updated: 2025-11-10T08:45:05Z has_accepted_license: '1' language: - iso: eng month: '10' oa: 1 oa_version: Published Version page: '141' project: - _id: 2533E772-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '638176' name: 'Big Splash: Efficient Simulation of Natural Phenomena at Extremely Large Scales' - _id: 34bc2376-11ca-11ed-8bc3-9a3b3961a088 grant_number: '101045083' name: Computational Discovery of Numerical Algorithms for Animation and Simulation of Natural Phenomena publication_identifier: isbn: - 978-3-99078-070-1 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '12846' relation: part_of_dissertation status: public - id: '12431' relation: part_of_dissertation status: public - id: '17361' relation: part_of_dissertation status: public - id: '20580' relation: part_of_dissertation status: public status: public supervisor: - first_name: Christopher J full_name: Wojtan, Christopher J id: 3C61F1D2-F248-11E8-B48F-1D18A9856A87 last_name: Wojtan orcid: 0000-0001-6646-5546 - first_name: Albert full_name: Chern, Albert last_name: Chern title: Symplectic-prequantum structures and dynamics on the codimension-2 shape space tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20575' abstract: - lang: eng text: "This thesis deals with eigenvalue and eigenvector universality results for random matrix ensembles equipped with non-trivial spatial structure. We consider both mean-field models with a general variance profile (Wigner-type matrices) and correlation structure (correlated matrices) among the entries, as well as non-mean-field random band matrices with bandwidth W >> N^(1/2).\r\n\r\nTo extract the universal properties of random matrix spectra and eigenvectors, we obtain concentration estimates for their resolvent, the local laws, which generalize the celebrated Wigner semicircle law for a broad class of random matrices to much finer spectral scales. The local laws hold for both a single resolvent as well as for products of multiple resolvents, known as resolvent chains, and express the remarkable approximately-deterministic behavior of these objects down to the microscopic scale.\r\n\r\nOur primary tool for establishing the local laws is the dynamical Zigzag strategy, which we develop in the setting of spatially-inhomogeneous random matrices. Our proof method systematically addresses the challenges arising from non-trivial spatial structures and is robust to all types of singularities in the spectrum, as we demonstrate in the correlated setting. Furthermore, we incorporate the analysis of the deterministic resolvent chain approximations into the dynamical framework of the Zigzag strategy, synthesizing a unified toolkit for establishing multi-resolvent local laws.\r\n\r\nUsing these methods, we prove complete eigenvector delocalization, the Eigenstate Thermalization Hypothesis, and Wigner-Dyson universality in the bulk for random band matrices down to the optimal bandwidth W >> N^(1/2). For mean-field ensembles, we establish universality of local eigenvalue statistics at the cups for random matrices with correlated entries, and the Eigenstate Thermalization Hypothesis for Wigner-type matrices in the bulk of the spectrum.\r\n\r\nFinally, this thesis also contains other applications of the multi-resolvent local laws to spatially-inhomogeneous random matrices, obtained prior to the development of the Zigzag strategy. In particular, we provide a complete analysis of mesoscopic linear-eigenvalue statistics of Wigner-type matrices in all spectral regimes, including the novel cusps, and rigorously establish the prethermalization phenomenon for deformed Wigner matrices.\r\n\r\nThe main body of this thesis consists of seven research papers (listed on page xi), each presented in a separate chapter with its own introduction and all relevant context, suitable to be read independently. We ask the reader’s indulgence for the repetitions in the historical overviews and other minor redundancies that remain among the chapters as a result. The overall Introduction, preceding the chapters, provides a condensed, informal summary of the main ideas and concepts at the core of these works.\r\n" acknowledgement: "The work comprising this thesis was supported by the ERC Advanced Grant \"RMTBeyond\"\r\nNo.101020331 awarded to my advisor." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Volodymyr full_name: Riabov, Volodymyr id: 1949f904-edfb-11eb-afb5-e2dfddabb93b last_name: Riabov citation: ama: Riabov V. Universality in random matrices with spatial structure. 2025. doi:10.15479/AT-ISTA-20575 apa: Riabov, V. (2025). Universality in random matrices with spatial structure. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20575 chicago: Riabov, Volodymyr. “Universality in Random Matrices with Spatial Structure.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20575. ieee: V. Riabov, “Universality in random matrices with spatial structure,” Institute of Science and Technology Austria, 2025. ista: Riabov V. 2025. Universality in random matrices with spatial structure. Institute of Science and Technology Austria. mla: Riabov, Volodymyr. Universality in Random Matrices with Spatial Structure. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20575. short: V. Riabov, Universality in Random Matrices with Spatial Structure, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-29T19:12:24Z date_published: 2025-11-03T00:00:00Z date_updated: 2026-04-07T12:32:20Z day: '3' ddc: - '515' - '519' degree_awarded: PhD department: - _id: GradSch - _id: LaEr doi: 10.15479/AT-ISTA-20575 ec_funded: 1 file: - access_level: open_access checksum: 6a0487b2b66bb35d44b394756d44b8b4 content_type: application/pdf creator: vriabov date_created: 2025-10-29T18:53:59Z date_updated: 2025-10-29T18:53:59Z file_id: '20577' file_name: riabov_thesis-pdfa.pdf file_size: 7536583 relation: main_file success: 1 - access_level: closed checksum: 224efda6bf9864d296a1e5e0124c1e8f content_type: application/x-zip-compressed creator: vriabov date_created: 2025-10-29T18:54:53Z date_updated: 2025-10-29T18:54:53Z file_id: '20578' file_name: manuscript.zip file_size: 17841612 relation: source_file file_date_updated: 2025-10-29T18:54:53Z has_accepted_license: '1' language: - iso: eng month: '11' oa: 1 oa_version: Published Version page: '436' project: - _id: 62796744-2b32-11ec-9570-940b20777f1d call_identifier: H2020 grant_number: '101020331' name: Random matrices beyond Wigner-Dyson-Mehta publication_identifier: isbn: - 978-3-99078-064-0 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '20322' relation: part_of_dissertation status: public - id: '18764' relation: part_of_dissertation status: public - id: '13317' relation: part_of_dissertation status: public - id: '19368' relation: part_of_dissertation status: deleted - id: '18554' relation: part_of_dissertation status: public - id: '20576' relation: part_of_dissertation status: public - id: '17174' relation: part_of_dissertation status: public - id: '19547' relation: part_of_dissertation status: public - id: '19598' relation: part_of_dissertation status: public status: public supervisor: - first_name: László full_name: Erdös, László id: 4DBD5372-F248-11E8-B48F-1D18A9856A87 last_name: Erdös orcid: 0000-0001-5366-9603 title: Universality in random matrices with spatial structure tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20737' acknowledged_ssus: - _id: Bio - _id: PreCl acknowledgement: "I also want to thank ISTA and the Austrian Science Fund FWF SFB F78 (F7805) for financially\r\nsupporting my research." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Raquel full_name: Casado Polanco, Raquel id: 15240fc1-dbcd-11ea-9d1d-ac5a786425fd last_name: Casado Polanco orcid: 0000-0001-8293-4568 citation: ama: Casado Polanco R. Role of NOTCH signaling in radial glial progenitor lineage progression. 2025. doi:10.15479/AT-ISTA-20737 apa: Casado Polanco, R. (2025). Role of NOTCH signaling in radial glial progenitor lineage progression. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20737 chicago: Casado Polanco, Raquel. “Role of NOTCH Signaling in Radial Glial Progenitor Lineage Progression.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20737. ieee: R. Casado Polanco, “Role of NOTCH signaling in radial glial progenitor lineage progression,” Institute of Science and Technology Austria, 2025. ista: Casado Polanco R. 2025. Role of NOTCH signaling in radial glial progenitor lineage progression. Institute of Science and Technology Austria. mla: Casado Polanco, Raquel. Role of NOTCH Signaling in Radial Glial Progenitor Lineage Progression. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20737. short: R. Casado Polanco, Role of NOTCH Signaling in Radial Glial Progenitor Lineage Progression, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-12-09T09:04:18Z date_published: 2025-12-09T00:00:00Z date_updated: 2026-04-14T08:16:58Z day: '09' ddc: - '570' degree_awarded: PhD department: - _id: GradSch - _id: SiHi doi: 10.15479/AT-ISTA-20737 file: - access_level: closed checksum: 71e0fdf4619b0d70d03657ad7348137d content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: rcasadop date_created: 2025-12-11T09:28:09Z date_updated: 2025-12-11T11:18:37Z file_id: '20793' file_name: 2025_CasadoPolanco_Raquel_Thesis.docx file_size: 78207207 relation: source_file - access_level: closed checksum: 58cf2f25c33567723bc754a019c3e396 content_type: application/pdf creator: rcasadop date_created: 2025-12-11T09:28:04Z date_updated: 2025-12-11T09:28:04Z embargo: 2026-12-01 embargo_to: open_access file_id: '20794' file_name: 2025_CasadoPolanco_Raquel_Thesis.pdf file_size: 6261874 relation: main_file file_date_updated: 2025-12-11T11:18:37Z has_accepted_license: '1' keyword: - NOTCH - radial glial progenitor - lineage progression - cortical development language: - iso: eng month: '12' oa_version: Published Version page: '133' project: - _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E grant_number: F7805 name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression publication_identifier: isbn: - 978-3-99078-072-5 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria status: public supervisor: - first_name: Simon full_name: Hippenmeyer, Simon id: 37B36620-F248-11E8-B48F-1D18A9856A87 last_name: Hippenmeyer orcid: 0000-0003-2279-1061 title: Role of NOTCH signaling in radial glial progenitor lineage progression type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20741' abstract: - lang: eng text: "Life on Earth emerged when biomacromolecules were membrane-enclosed in a confined space where many essential chemical reactions were more likely to happen and thereby accelerate evolution. These kinds of membranes separated internal reactions from the outside chaos while staying flexible so that those primordial cells can move, adopt their shape and, most importantly, propagate. Such membrane plasticity still remains a defining feature of all modern cell types. This remarkable ability to change their shape is most prominently observed during their propagation (i.e., cell division). Throughout division, a cell undergoes drastic change in its shape, usually at the middle of the cell, pulling the two opposite membrane sides inward, closer to each other, and, finally, culminating in pinching off to separate the cell into two daughter cells. To achieve this, a cell needs to employ a protein machinery, usually termed divisome, that can coordinate all necessary intracellular processes with membrane remodelling and synthesis of other extracellular structures that decorate a cell. The focus of this dissertation is a membrane-remodelling FtsZ system that is present across all domains of life. FtsZ forms filaments that further self-organize into ring-like structures at the cell septum and together with other division proteins perform cell envelope synthesis and constriction. However, there are still knowledge gaps in our mechanistic understanding of division in both archaea and bacteria. My work presented in this dissertation centres around a simple yet not well understood question: How is the divisome positioned correctly at the mid-cell? To achieve the proper positioning, the divisome needs to (i) be recruited to the mid-cell and (ii) localized orthogonally to the long cell axis. I tackle these processes in two different systems by applying an in vitro biochemical bottom-up reconstitution approach. I use purified components of Haloferax volcanii and Escherichia coli divisome to explore how divisome is recruited to the mid-cell in archaea and how the Z-ring positions orthogonally to the long cell axis in bacteria, respectively. \r\n\r\nFirstly, I collaborate with archaeal cell and structural biologists to explore the assembly of early division proteins in two FtsZ-containing archaeon H. volcanii, a standard model system for understudied archaeal organisms. I particularly address the hierarchy of interactions that allow a tripartite complex formation (SepF-CdpB1-CdpB2) and how the hierarchy of interactions ultimately leads to the recruitment of FtsZ filaments to the septum. This part of work has been published in (Nußbaum et al., 2024). In collaboration with evolutionary biologists, I shed light on ancient features that archaeal divisome has retained to this day and also speculate on a property that it might have lost during the course of evolution. \r\n\r\nNext, I switch my attention to E. coli divisome. Particularly, I address the FtsZ’s intrinsic biophysical property that drives the Z-ring diameter, and thereby the perpendicular orientation of the Z-ring to the long cell axis based on suggested membrane curvature sensing mechanism (Vanhille-Campos et al., 2024). This property allows formation of different Z-ring diameters that match the variety of cell diameters present in prokaryotes. The results showcase that the distribution of charged amino acids in the intrinsically disordered linker at the C-terminus (CTL) of FtsZ is the major determining factor of Z-ring diameter with inter-CTL interactions as an underlying mechanism. \r\n\r\nFinally, I thoroughly explain the methodology I used to address the abovementioned projects, and I finish with a discussion on how early archaeal divisome assembly and curvature sensing mechanism in bacteria, at first sight unrelated topics, are interconnected and important groundwork for both fundamental and translational research. " acknowledged_ssus: - _id: Bio alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Marko full_name: Kojic, Marko id: 73e7ecd4-dc85-11ea-9058-88a16394b160 last_name: Kojic orcid: 0000-0001-7244-8128 citation: ama: Kojic M. Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria. 2025. doi:10.15479/AT-ISTA-20741 apa: Kojic, M. (2025). Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20741 chicago: Kojic, Marko. “Towards Understanding the Assembly Mechanisms of the Z-Ring in Archaea and Bacteria.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20741. ieee: M. Kojic, “Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria,” Institute of Science and Technology Austria, 2025. ista: Kojic M. 2025. Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria. Institute of Science and Technology Austria. mla: Kojic, Marko. Towards Understanding the Assembly Mechanisms of the Z-Ring in Archaea and Bacteria. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20741. short: M. Kojic, Towards Understanding the Assembly Mechanisms of the Z-Ring in Archaea and Bacteria, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-12-09T13:08:11Z date_published: 2025-12-09T00:00:00Z date_updated: 2026-04-07T12:27:58Z day: '09' ddc: - '572' degree_awarded: PhD department: - _id: GradSch - _id: MaLo doi: 10.15479/AT-ISTA-20741 file: - access_level: closed checksum: a3643d07e93134b2490a566b02a4517d content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: mkojic date_created: 2025-12-10T13:09:58Z date_updated: 2025-12-10T13:09:58Z file_id: '20774' file_name: 2025_marko_kojic_thesis.docx file_size: 142876975 relation: source_file - access_level: open_access checksum: 0a096f0af6ccc3a8329d5bb8797ad533 content_type: application/pdf creator: mkojic date_created: 2025-12-10T13:09:38Z date_updated: 2025-12-10T13:09:38Z file_id: '20775' file_name: 2025_marko_kojic_thesis.pdf file_size: 8597045 relation: main_file success: 1 file_date_updated: 2025-12-10T13:09:58Z has_accepted_license: '1' language: - iso: eng month: '12' oa: 1 oa_version: Published Version publication_identifier: isbn: - 978-3-99078-073-2 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '15118' relation: part_of_dissertation status: public status: public supervisor: - first_name: Martin full_name: Loose, Martin id: 462D4284-F248-11E8-B48F-1D18A9856A87 last_name: Loose orcid: 0000-0001-7309-9724 title: Towards understanding the assembly mechanisms of the Z-ring in Archaea and Bacteria tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20364' acknowledged_ssus: - _id: LifeSc - _id: Bio acknowledgement: "Plant Facility,\r\nProtein Service Facility" alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Caterina full_name: Giannini, Caterina id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4 last_name: Giannini citation: ama: Giannini C. Nuclear and cell surface auxin signaling in A. thaliana developmental transitions. 2025. doi:10.15479/AT-ISTA-20364 apa: Giannini, C. (2025). Nuclear and cell surface auxin signaling in A. thaliana developmental transitions. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20364 chicago: Giannini, Caterina. “Nuclear and Cell Surface Auxin Signaling in A. Thaliana Developmental Transitions.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20364. ieee: C. Giannini, “Nuclear and cell surface auxin signaling in A. thaliana developmental transitions,” Institute of Science and Technology Austria, 2025. ista: Giannini C. 2025. Nuclear and cell surface auxin signaling in A. thaliana developmental transitions. Institute of Science and Technology Austria. mla: Giannini, Caterina. Nuclear and Cell Surface Auxin Signaling in A. Thaliana Developmental Transitions. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20364. short: C. Giannini, Nuclear and Cell Surface Auxin Signaling in A. Thaliana Developmental Transitions, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-09-19T12:23:38Z date_published: 2025-09-19T00:00:00Z date_updated: 2026-07-06T12:51:13Z day: '19' ddc: - '580' degree_awarded: PhD department: - _id: GradSch - _id: JiFr - _id: MaLo doi: 10.15479/AT-ISTA-20364 file: - access_level: closed checksum: 536ba1701453b0b2346be14c046b2911 content_type: application/pdf creator: cgiannin date_created: 2025-09-24T14:46:34Z date_updated: 2025-09-30T14:31:29Z embargo: 2026-09-30 embargo_to: open_access file_id: '20390' file_name: 2025_Giannini_Caterina_Thesis...pdf file_size: 14278965 relation: main_file - access_level: closed checksum: 192f55262f2da2ea0a59d9c23a1b8573 content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: cgiannin date_created: 2025-09-24T14:46:35Z date_updated: 2025-09-24T14:46:35Z file_id: '20391' file_name: 2025_Giannini_Caterina_Thesis...docx file_size: 24499022 relation: source_file file_date_updated: 2025-09-30T14:31:29Z has_accepted_license: '1' keyword: - Auxin Signaling - Plant Development language: - iso: eng month: '09' oa_version: Published Version page: '151' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '12291' relation: part_of_dissertation status: public - id: '19399' relation: part_of_dissertation status: public status: public supervisor: - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 title: Nuclear and cell surface auxin signaling in A. thaliana developmental transitions type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20441' abstract: - lang: eng text: "Epithelial spreading plays a pivotal role in the development of organisms especially those\r\nsuch as zebrafish which require the epithelial enveloping layer (EVL) to spread to cover the\r\nsubstantial yolk surface during gastrulation. Epiboly requires the transition of the epithelium\r\nwith cuboidal cells to form a thin, flat squamous epithelial sheet. During this transition, the\r\ncells show tissue-scale mechanosensation with mechanisms such as direct mechanical control\r\nover the axis of cell division.\r\nCytoskeletal intermediate filaments play a crucial role in vertebrate cells, not only facilitating\r\nmechanical stability but also helping facilitate the mechanosensitive response of the cell.\r\nMechanosenstivity displayed by intermediate filaments is due not just to their interesting\r\nphysical properties but also to their interactions with other cytoskeletal elements such as actin\r\nand microtubules. Keratin is the predominant intermediate filament expressed in the EVL.\r\nIt expresses concomitantly with the gastrulation movements of the developing embryo. Our\r\nwork focuses on understanding the role and dynamics of the keratin cytoskeletal network in\r\nmodulating the physical aspects of EVL spreading. We demonstrated with the combination of\r\nphysical characterisation and manipulations of the EVL, utilising a variety of biophysical tools\r\nand microscopy, the mechanistic role of keratin in tissue spreading.\r\nGenerating novel genetic morphants and mutants, we probe the effect that the loss of the\r\nkeratin network has on the physiology of the epithelium and the developing embryo. We\r\nshow that the changing organisation of the keratin network is important for changing EVL\r\nphysical properties as the stress imposed on the EVL increases during epiboly. By modelling\r\nthe epithelium, we study how the mechanical heterogeneity in an epithelium can feed back into\r\na mechanical loop to the maturation of the keratin network and hence affect the mechanics\r\nof the epithelium. However, unlike what would be predicted by the effect of intermediate\r\nfilaments in acting as a security belt and increasing the resistance of the epithelium, we observe\r\nthat loss of keratin leads to a delay in the EVL movement. Using both local aspirations of the\r\nYSL and EVL ablations, we demonstrate the mechanistic facilitation of actin mechanosensation\r\nin a keratin-dependent manner.\r\nFurthermore, using chemical inhibitors of microtubule polymerisation, we provide insight into\r\nthe mechanisms underlying the organisation and distribution of keratin. Interestingly, the\r\nphenotype observed upon this loss of microtubules shows that keratins interact with the nucleus\r\nthrough microtubular interactions. Together with these diverse observations, we describe\r\nthe mechanosensory feedback between resilience and that is critical for uniform and robust\r\nspreading of the epithelium." acknowledged_ssus: - _id: Bio - _id: LifeSc acknowledgement: "I would also like to thank the LSF and Cryo facility at ISTA, which have been helpful in my\r\nexperiments. I would also like to acknowledge FWF, grant DOI 10.55776/PAT5044023 and JKU Nanocell grant DOI \r\n10.55776/W1250 for providing funding for my PhD research. EMBO and FWF for providing funding for travel grants to attend conferences." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Suyash full_name: Naik, Suyash id: 2C0B105C-F248-11E8-B48F-1D18A9856A87 last_name: Naik orcid: 0000-0001-8421-5508 citation: ama: Naik S. Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. 2025. doi:10.15479/AT-ISTA-20441 apa: Naik, S. (2025). Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20441 chicago: Naik, Suyash. “Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20441. ieee: S. Naik, “Keratins act as global coordinators of tissue spreading through mechanosensitive feedback,” Institute of Science and Technology Austria, 2025. ista: Naik S. 2025. Keratins act as global coordinators of tissue spreading through mechanosensitive feedback. Institute of Science and Technology Austria. mla: Naik, Suyash. Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20441. short: S. Naik, Keratins Act as Global Coordinators of Tissue Spreading through Mechanosensitive Feedback, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-10T14:58:30Z date_published: 2025-10-12T00:00:00Z date_updated: 2026-07-06T12:51:41Z day: '12' ddc: - '596' - '597' - '532' degree_awarded: PhD department: - _id: GradSch - _id: CaHe - _id: EdHa doi: 10.15479/AT-ISTA-20441 file: - access_level: open_access checksum: 2892f04d4a5c18677871c3e06ac1244a content_type: application/pdf creator: snaik date_created: 2025-10-28T13:10:08Z date_updated: 2025-10-28T13:10:08Z file_id: '20567' file_name: Thesis_PDFA_.pdf file_size: 6846189 relation: main_file success: 1 - access_level: open_access checksum: 15934d4465cd0e9b7c32678da9a33a2f content_type: application/zip creator: snaik date_created: 2025-10-28T13:10:26Z date_updated: 2025-10-28T13:10:26Z file_id: '20568' file_name: Thesis.zip file_size: 8839300 relation: source_file file_date_updated: 2025-10-28T13:10:26Z has_accepted_license: '1' language: - iso: eng license: https://creativecommons.org/licenses/by-sa/4.0/ month: '10' oa: 1 oa_version: Published Version page: '105' project: - _id: 8f060199-16d5-11f0-9cad-f3253b266c46 grant_number: PAT 5044023 name: Keratins in epithelial tissue spreading - _id: 25AA5F24-B435-11E9-9278-68D0E5697425 call_identifier: FWF grant_number: W 1250-B20 name: Nano-Analytics of Cellular Systems publication_identifier: isbn: - 978-3-99078-069-5 issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '20465' relation: part_of_dissertation status: public status: public supervisor: - first_name: Carl-Philipp J full_name: Heisenberg, Carl-Philipp J id: 39427864-F248-11E8-B48F-1D18A9856A87 last_name: Heisenberg orcid: 0000-0002-0912-4566 - first_name: Edouard B full_name: Hannezo, Edouard B id: 3A9DB764-F248-11E8-B48F-1D18A9856A87 last_name: Hannezo orcid: 0000-0001-6005-1561 title: Keratins act as global coordinators of tissue spreading through mechanosensitive feedback tmp: image: /images/cc_by_sa.png legal_code_url: https://creativecommons.org/licenses/by-sa/4.0/legalcode name: Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0) short: CC BY-SA (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '19478' acknowledged_ssus: - _id: Bio - _id: LifeSc acknowledgement: "This project was funded by the European Research Council Advanced Grant (ETAP-742985),\r\nEuropean Research Council (ERC; 101142681 CYNIPS), Austrian Science Fund (FWF; P\r\n37051-B)." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Huihuang full_name: Chen, Huihuang id: 83c96512-15b2-11ec-abd3-b7eede36184f last_name: Chen citation: ama: Chen H. The cAMP second messenger in auxin signalling. 2025. doi:10.15479/AT-ISTA-19478 apa: Chen, H. (2025). The cAMP second messenger in auxin signalling. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-19478 chicago: Chen, Huihuang. “The CAMP Second Messenger in Auxin Signalling.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-19478. ieee: H. Chen, “The cAMP second messenger in auxin signalling,” Institute of Science and Technology Austria, 2025. ista: Chen H. 2025. The cAMP second messenger in auxin signalling. Institute of Science and Technology Austria. mla: Chen, Huihuang. The CAMP Second Messenger in Auxin Signalling. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-19478. short: H. Chen, The CAMP Second Messenger in Auxin Signalling, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-04-04T07:48:24Z date_published: 2025-04-04T00:00:00Z date_updated: 2026-07-06T12:58:58Z day: '04' ddc: - '580' degree_awarded: PhD department: - _id: GradSch - _id: JiFr doi: 10.15479/AT-ISTA-19478 ec_funded: 1 file: - access_level: closed checksum: b154973663a1bba505683faab7ae5ead content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document creator: hchen date_created: 2025-04-08T08:00:07Z date_updated: 2025-04-08T08:22:37Z file_id: '19526' file_name: Thesis_0403_Huihuang.docx file_size: 16344814 relation: source_file - access_level: closed checksum: 0099565f024388830c125ec17375c1a0 content_type: application/pdf creator: hchen date_created: 2025-04-08T08:00:06Z date_updated: 2025-04-09T13:53:38Z embargo: 2026-10-08 embargo_to: local file_id: '19527' file_name: Thesis_0406_PDFA_Huihuang_1.pdf file_size: 8482147 relation: main_file file_date_updated: 2025-04-09T13:53:38Z has_accepted_license: '1' language: - iso: eng month: '04' oa_version: Published Version page: '118' project: - _id: 261099A6-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742985' name: Tracing Evolution of Auxin Transport and Polarity in Plants - _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6 grant_number: P37051 name: Guanylate cyclase activity of TIR1/AFBs auxin receptors - _id: 8f347782-16d5-11f0-9cad-8c19706ee739 grant_number: '101142681' name: Cyclic nucleotides as second messengers in plants publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '19421' relation: part_of_dissertation status: public - id: '13212' relation: part_of_dissertation status: public status: public supervisor: - first_name: Jiří full_name: Friml, Jiří id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 title: The cAMP second messenger in auxin signalling type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20920' abstract: - lang: eng text: "Verifiable Delay Functions (VDFs) introduced by Boneh et al. (CRYPTO'18) are functions that require a prescribed number of sequential steps T to evaluate, yet their output can be verified in time much faster than T. Since their introduction, VDFs have gained a lot of attention due to their applications in blockchain protocols, randomness beacons, timestamping and deniability. This thesis explores the theory and applications of VDFs, focusing on enhancing their soundness, efficiency and practicality.\r\n\r\nThe only practical VDFs known to date are based on repeated squaring in hidden order groups. Consider the function VDF(x,T)=x^(2^T).\r\nThe iterated squaring assumption states that, for a random group element x, the result of VDF cannot be computed significantly faster than performing T sequential squarings if the group order is unknown. To make the result verifiable a prover can compute a proof of exponentiation (PoE) \\pi. Given \\pi, the output of VDF can be verified in time much less than T.\r\n\r\nWe first present new constructions of statistically sound proofs of exponentiation, which are an important building block in the construction of SNARKs (Succinct Non-Interactive Argument of Knowledge). Statistical soundness means that the proofs remain secure against computationally unbounded adversaries, in particular, it remains secure even when the group order is known. We thereby address limitations in previous PoE protocols which either required (non-standard) hardness assumptions or a lot of parallel repetitions. Our construction significantly reduces the proof size of statistically sound PoEs that allow for a structured exponent, which leads to better efficiency of SNARKs and other applications.\r\n\r\nSecondly, we introduce improved batching techniques for PoEs, which allow multiple proofs to be aggregated and verified with minimal overhead. These protocols optimize communication and computation complexity in large-scale blockchain environments and enable scalable remote benchmarking of parallel computation resources.\r\n\r\nWe then construct VDFs with enhanced properties such as zero-knowledge and watermarkability. It was shown by Arun, Bonneau and Clark (ASIACRYPT'22) that these features enable new cryptographic primitives called short-lived proofs and signatures. The validity of such proofs and signatures expires after a predefined amount of time T, i.e., they are deniable after time T. Our constructions improve upon the constructions by Arun, Bonneau and Clark in several dimensions (faster forging times, arguably weaker assumptions).\r\n\r\nFinally, we apply PoEs in the realm of primality testing, providing cryptographically sound proofs of non-primality for large Proth numbers. This work gives a surprising application of VDFs in the area of computational number theory.\r\n\r\nTogether, our contributions advance both the theoretical foundations and the real-world usability of VDFs in general and in particular of PoEs, making them more adaptable and secure for current and emerging cryptographic applications." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Charlotte full_name: Hoffmann, Charlotte id: 0f78d746-dc7d-11ea-9b2f-83f92091afe7 last_name: Hoffmann orcid: 0000-0003-2027-5549 citation: ama: Hoffmann C. Theory and applications of verifiable delay functions. 2025. doi:10.15479/AT-ISTA-20920 apa: Hoffmann, C. (2025). Theory and applications of verifiable delay functions. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20920 chicago: Hoffmann, Charlotte. “Theory and Applications of Verifiable Delay Functions.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20920. ieee: C. Hoffmann, “Theory and applications of verifiable delay functions,” Institute of Science and Technology Austria, 2025. ista: Hoffmann C. 2025. Theory and applications of verifiable delay functions. Institute of Science and Technology Austria. mla: Hoffmann, Charlotte. Theory and Applications of Verifiable Delay Functions. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20920. short: C. Hoffmann, Theory and Applications of Verifiable Delay Functions, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2026-01-02T10:46:47Z date_published: 2025-12-31T00:00:00Z date_updated: 2026-07-06T13:15:27Z day: '31' ddc: - '004' degree_awarded: PhD department: - _id: GradSch - _id: KrPi doi: 10.15479/AT-ISTA-20920 file: - access_level: closed checksum: 8a099fbf54963bd0be38f7ce73658682 content_type: application/x-zip-compressed creator: choffman date_created: 2026-01-02T10:39:16Z date_updated: 2026-01-02T10:39:16Z file_id: '20921' file_name: 2025_Hoffmann_Charlotte_Source.zip file_size: 8355494 relation: source_file - access_level: open_access checksum: 9521c07bfb2bb5b14a49c09fcfc96474 content_type: application/pdf creator: choffman date_created: 2026-01-02T10:39:26Z date_updated: 2026-01-02T10:39:26Z file_id: '20922' file_name: 2025_Hoffmann_Charlotte_Thesis.pdf file_size: 2258804 relation: main_file success: 1 file_date_updated: 2026-01-02T10:39:26Z has_accepted_license: '1' language: - iso: eng month: '12' oa: 1 oa_version: Published Version page: '116' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '13143' relation: part_of_dissertation status: public - id: '12176' relation: part_of_dissertation status: public - id: '20556' relation: earlier_version status: public - id: '19778' relation: part_of_dissertation status: public - id: '20701' relation: part_of_dissertation status: public status: public supervisor: - first_name: Krzysztof Z full_name: Pietrzak, Krzysztof Z id: 3E04A7AA-F248-11E8-B48F-1D18A9856A87 last_name: Pietrzak orcid: 0000-0002-9139-1654 title: Theory and applications of verifiable delay functions tmp: image: /images/cc_by_nc_sa.png legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) short: CC BY-NC-SA (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ... --- OA_place: publisher _id: '20556' abstract: - lang: eng text: "Verifiable Delay Functions (VDFs) introduced by Boneh et al. (CRYPTO'18) are functions that require a prescribed number of sequential steps T to evaluate, yet their output can be verified in time much faster than T. Since their introduction, VDFs have gained a lot of attention due to their applications in blockchain protocols, randomness beacons, timestamping and deniability. This thesis explores the theory and applications of VDFs, focusing on enhancing their soundness, efficiency and practicality.\r\n\r\nThe only practical VDFs known to date are based on repeated squaring in hidden order groups. Consider the function VDF(x,T)=x^(2^T).\r\nThe iterated squaring assumption states that, for a random group element x, the result of VDF cannot be computed significantly faster than performing T sequential squarings if the group order is unknown. To make the result verifiable a prover can compute a proof of exponentiation (PoE) \\pi. Given \\pi, the output of VDF can be verified in time much less than T.\r\n\r\nWe first present new constructions of statistically sound proofs of exponentiation, which are an important building block in the construction of SNARKs (Succinct Non-Interactive Argument of Knowledge). Statistical soundness means that the proofs remain secure against computationally unbounded adversaries, in particular, it remains secure even when the group order is known. We thereby address limitations in previous PoE protocols which either required (non-standard) hardness assumptions or a lot of parallel repetitions. Our construction significantly reduces the proof size of statistically sound PoEs that allow for a structured exponent, which leads to better efficiency of SNARKs and other applications.\r\n\r\nSecondly, we introduce improved batching techniques for PoEs, which allow multiple proofs to be aggregated and verified with minimal overhead. These protocols optimize communication and computation complexity in large-scale blockchain environments and enable scalable remote benchmarking of parallel computation resources.\r\n\r\nWe then construct VDFs with enhanced properties such as zero-knowledge and watermarkability. It was shown by Arun, Bonneau and Clark (ASIACRYPT'22) that these features enable new cryptographic primitives called short-lived proofs and signatures. The validity of such proofs and signatures expires after a predefined amount of time T, i.e., they are deniable after time T. Our constructions improve upon the constructions by Arun, Bonneau and Clark in several dimensions (faster forging times, arguably weaker assumptions).\r\n\r\nFinally, we apply PoEs in the realm of primality testing, providing cryptographically sound proofs of non-primality for large Proth numbers. This work gives a surprising application of VDFs in the area of computational number theory.\r\n\r\nTogether, our contributions advance both the theoretical foundations and the real-world usability of VDFs in general and in particular of PoEs, making them more adaptable and secure for current and emerging cryptographic applications." alternative_title: - ISTA Thesis article_processing_charge: No author: - first_name: Charlotte full_name: Hoffmann, Charlotte id: 0f78d746-dc7d-11ea-9b2f-83f92091afe7 last_name: Hoffmann orcid: 0000-0003-2027-5549 citation: ama: Hoffmann C. Theory and applications of verifiable delay functions. 2025. doi:10.15479/AT-ISTA-20556 apa: Hoffmann, C. (2025). Theory and applications of verifiable delay functions. Institute of Science and Technology Austria. https://doi.org/10.15479/AT-ISTA-20556 chicago: Hoffmann, Charlotte. “Theory and Applications of Verifiable Delay Functions.” Institute of Science and Technology Austria, 2025. https://doi.org/10.15479/AT-ISTA-20556. ieee: C. Hoffmann, “Theory and applications of verifiable delay functions,” Institute of Science and Technology Austria, 2025. ista: Hoffmann C. 2025. Theory and applications of verifiable delay functions. Institute of Science and Technology Austria. mla: Hoffmann, Charlotte. Theory and Applications of Verifiable Delay Functions. Institute of Science and Technology Austria, 2025, doi:10.15479/AT-ISTA-20556. short: C. Hoffmann, Theory and Applications of Verifiable Delay Functions, Institute of Science and Technology Austria, 2025. corr_author: '1' date_created: 2025-10-27T14:16:56Z date_published: 2025-10-31T00:00:00Z date_updated: 2026-07-06T13:15:27Z day: '31' ddc: - '004' degree_awarded: PhD department: - _id: GradSch - _id: KrPi doi: 10.15479/AT-ISTA-20556 file: - access_level: closed checksum: 1fffa4e2c33dd0b9f883d615504a2858 content_type: application/pdf creator: choffman date_created: 2025-10-28T14:33:03Z date_updated: 2026-01-08T14:11:39Z file_id: '20573' file_name: 2025_Hoffmann_Charlotte_Thesis.pdf file_size: 2259304 relation: main_file - access_level: closed checksum: 076ea98a1f0a86c3bbc990b6b9460dc2 content_type: application/x-zip-compressed creator: choffman date_created: 2025-10-28T14:35:06Z date_updated: 2025-11-11T09:34:54Z file_id: '20574' file_name: 2025_Hoffmann_Charlotte_Source.zip file_size: 9987633 relation: source_file file_date_updated: 2026-01-08T14:11:39Z has_accepted_license: '1' language: - iso: eng month: '10' oa_version: Published Version page: '116' publication_identifier: issn: - 2663-337X publication_status: published publisher: Institute of Science and Technology Austria related_material: record: - id: '13143' relation: part_of_dissertation status: public - id: '12176' relation: part_of_dissertation status: public - id: '20920' relation: later_version status: public - id: '19778' relation: part_of_dissertation status: public - id: '20701' relation: part_of_dissertation status: public status: public supervisor: - first_name: Krzysztof Z full_name: Pietrzak, Krzysztof Z id: 3E04A7AA-F248-11E8-B48F-1D18A9856A87 last_name: Pietrzak orcid: 0000-0002-9139-1654 title: Theory and applications of verifiable delay functions tmp: image: /images/cc_by_nc_sa.png legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) short: CC BY-NC-SA (4.0) type: dissertation user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd year: '2025' ...