---
OA_place: publisher
_id: '14510'
abstract:
- lang: eng
text: "Clathrin-mediated endocytosis (CME) is vital for the regulation of plant
growth and\r\ndevelopment by controlling plasma membrane protein composition and
cargo uptake. CME\r\nrelies on the precise recruitment control of protein regulators
for vesicle maturation and\r\nrelease. During the early stages of endocytosis,
an area of flat membrane is remodelled by\r\nproteins to create a spherical vesicle
against intracellular forces. After the Clathrin-coated\r\nvesicle (CCV) is fully
formed, scission machinery releases it from the plasma membrane,\r\nand cargo
proceeds for recycling or degradation through early endosomes / Trans Golgi\r\nnetwork.
Protein machineries that mediate membrane bending and vesicle release in plants\r\nare
unknown. However, studies show, that plant endocytosis is actin independent, thus\r\nindicating
that plants utilize a unique mechanism to mediate membrane bending against highturgor
pressure compared to other model systems. First, by using biochemical and advanced\r\nlive
microscopy approaches we investigate the TPLATE complex, a plant-specific\r\nendocytosis
protein complex. We found that TPLATE is peripherally associated with\r\nclathrin-coated
vesicles and localises at the rim of endocytosis events. Next, our study of\r\nplant
Dynamin-related protein 1C (DRP1C), which was hypothesised previously to play
a\r\nrole in vesicle release, shows the recruitment of the protein already at
the early stages of\r\nendocytosis. Moreover, DRP1C assembles into organised ring-like
structures and is able to\r\ninduce membrane deformation and tubulation, suggesting
its role also in membrane bending\r\nduring early CME. Based on the data from
mammalian and yeast systems, plant DynaminRelated Proteins 2 and SH3P2 protein
are strong candidates to be part of the plant vesicle\r\nscission machinery; however,
their precise role in plant CME has not been yet elucidated.\r\nHere, we characterised
DRP2s and SH3P2 roles in CME by combining high-resolution\r\nimaging of endocytic
events in vivo and protein characterisation. Although DRP2s and\r\nSH3P2 arrive
together during late CME and physically interact, genetic analysis using\r\n∆sh3p1,2,3
mutant and complementation with non-DRP2-interacting SH3P2 variants suggest\r\nthat
SH3P2 does not directly recruit DRP2s to the site of endocytosis. Summarising
our\r\nresearch, these observations provide new important insights into the mechanism
of plant\r\nCME and show that, despite plants posses many homologues of mammalian
and yeast CME\r\ncomponents, they do not necessarily act in the same manner. "
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: LifeSc
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
citation:
ama: Gnyliukh N. Mechanism of clathrin-coated vesicle formation during endocytosis
in plants. 2023. doi:10.15479/at:ista:14510
apa: Gnyliukh, N. (2023). Mechanism of clathrin-coated vesicle formation during
endocytosis in plants. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:14510
chicago: Gnyliukh, Nataliia. “Mechanism of Clathrin-Coated Vesicle Formation during
Endocytosis in Plants.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:14510.
ieee: N. Gnyliukh, “Mechanism of clathrin-coated vesicle formation during endocytosis
in plants,” Institute of Science and Technology Austria, 2023.
ista: Gnyliukh N. 2023. Mechanism of clathrin-coated vesicle formation during endocytosis
in plants. Institute of Science and Technology Austria.
mla: Gnyliukh, Nataliia. Mechanism of Clathrin-Coated Vesicle Formation during
Endocytosis in Plants. Institute of Science and Technology Austria, 2023,
doi:10.15479/at:ista:14510.
short: N. Gnyliukh, Mechanism of Clathrin-Coated Vesicle Formation during Endocytosis
in Plants, Institute of Science and Technology Austria, 2023.
corr_author: '1'
date_created: 2023-11-10T09:10:06Z
date_published: 2023-11-10T00:00:00Z
date_updated: 2026-04-27T22:30:30Z
day: '10'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: JiFr
- _id: MaLo
doi: 10.15479/at:ista:14510
ec_funded: 1
file:
- access_level: closed
checksum: 3d5e680bfc61f98e308c434f45cc9bd6
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: ngnyliuk
date_created: 2023-11-20T09:18:51Z
date_updated: 2024-11-23T23:30:38Z
embargo_to: open_access
file_id: '14567'
file_name: Thesis_Gnyliukh_final_08_11_23.docx
file_size: 20824903
relation: source_file
- access_level: open_access
checksum: bfc96d47fc4e7e857dd71656097214a4
content_type: application/pdf
creator: ngnyliuk
date_created: 2023-11-20T09:23:11Z
date_updated: 2024-11-23T23:30:38Z
embargo: 2024-11-23
file_id: '14568'
file_name: Thesis_Gnyliukh_final_20_11_23.pdf
file_size: 24871844
relation: main_file
file_date_updated: 2024-11-23T23:30:38Z
has_accepted_license: '1'
keyword:
- Clathrin-Mediated Endocytosis
- vesicle scission
- Dynamin-Related Protein 2
- SH3P2
- TPLATE complex
- Total internal reflection fluorescence microscopy
- Arabidopsis thaliana
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
page: '180'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication_identifier:
isbn:
- 978-3-99078-037-4
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '14591'
relation: part_of_dissertation
status: public
- id: '9887'
relation: part_of_dissertation
status: public
- id: '8139'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
title: Mechanism of clathrin-coated vesicle formation during endocytosis in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2023'
...