---
_id: '8487'
abstract:
- lang: eng
  text: Following unidirectional biophysical events such as the folding of proteins
    or the equilibration of binding interactions, requires experimental methods that
    yield information at both atomic-level resolution and at high repetition rates.
    Toward this end a number of different approaches enabling the rapid acquisition
    of 2D NMR spectra have been recently introduced, including spatially encoded “ultrafast”
    2D NMR spectroscopy and SOFAST HMQC NMR. Whereas the former accelerates acquisitions
    by reducing the number of scans that are necessary for completing arbitrary 2D
    NMR experiments, the latter operates by reducing the delay between consecutive
    scans while preserving sensitivity. Given the complementarities between these
    two approaches it seems natural to combine them into a single tool, enabling the
    acquisition of full 2D protein NMR spectra at high repetition rates. We demonstrate
    here this capability with the introduction of “ultraSOFAST” HMQC NMR, a spatially
    encoded and relaxation-optimized approach that can provide 2D protein correlation
    spectra at ∼1 s repetition rates for samples in the ∼2 mM concentration range.
    The principles, relative advantages, and current limitations of this new approach
    are discussed, and its application is exemplified with a study of the fast hydrogen−deuterium
    exchange characterizing amide sites in Ubiquitin.
article_processing_charge: No
article_type: original
author:
- first_name: Maayan
  full_name: Gal, Maayan
  last_name: Gal
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Bernhard
  full_name: Brutscher, Bernhard
  last_name: Brutscher
- first_name: Lucio
  full_name: Frydman, Lucio
  last_name: Frydman
citation:
  ama: Gal M, Schanda P, Brutscher B, Frydman L. UltraSOFAST HMQC NMR and the repetitive
    acquisition of 2D protein spectra at Hz rates. <i>Journal of the American Chemical
    Society</i>. 2007;129(5):1372-1377. doi:<a href="https://doi.org/10.1021/ja066915g">10.1021/ja066915g</a>
  apa: Gal, M., Schanda, P., Brutscher, B., &#38; Frydman, L. (2007). UltraSOFAST
    HMQC NMR and the repetitive acquisition of 2D protein spectra at Hz rates. <i>Journal
    of the American Chemical Society</i>. American Chemical Society. <a href="https://doi.org/10.1021/ja066915g">https://doi.org/10.1021/ja066915g</a>
  chicago: Gal, Maayan, Paul Schanda, Bernhard Brutscher, and Lucio Frydman. “UltraSOFAST
    HMQC NMR and the Repetitive Acquisition of 2D Protein Spectra at Hz Rates.” <i>Journal
    of the American Chemical Society</i>. American Chemical Society, 2007. <a href="https://doi.org/10.1021/ja066915g">https://doi.org/10.1021/ja066915g</a>.
  ieee: M. Gal, P. Schanda, B. Brutscher, and L. Frydman, “UltraSOFAST HMQC NMR and
    the repetitive acquisition of 2D protein spectra at Hz rates,” <i>Journal of the
    American Chemical Society</i>, vol. 129, no. 5. American Chemical Society, pp.
    1372–1377, 2007.
  ista: Gal M, Schanda P, Brutscher B, Frydman L. 2007. UltraSOFAST HMQC NMR and the
    repetitive acquisition of 2D protein spectra at Hz rates. Journal of the American
    Chemical Society. 129(5), 1372–1377.
  mla: Gal, Maayan, et al. “UltraSOFAST HMQC NMR and the Repetitive Acquisition of
    2D Protein Spectra at Hz Rates.” <i>Journal of the American Chemical Society</i>,
    vol. 129, no. 5, American Chemical Society, 2007, pp. 1372–77, doi:<a href="https://doi.org/10.1021/ja066915g">10.1021/ja066915g</a>.
  short: M. Gal, P. Schanda, B. Brutscher, L. Frydman, Journal of the American Chemical
    Society 129 (2007) 1372–1377.
date_created: 2020-09-18T10:13:27Z
date_published: 2007-01-10T00:00:00Z
date_updated: 2021-01-12T08:19:37Z
day: '10'
doi: 10.1021/ja066915g
extern: '1'
intvolume: '       129'
issue: '5'
keyword:
- Colloid and Surface Chemistry
- Biochemistry
- General Chemistry
- Catalysis
language:
- iso: eng
month: '01'
oa_version: None
page: 1372-1377
publication: Journal of the American Chemical Society
publication_identifier:
  issn:
  - 0002-7863
  - 1520-5126
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
status: public
title: UltraSOFAST HMQC NMR and the repetitive acquisition of 2D protein spectra at
  Hz rates
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 129
year: '2007'
...
---
_id: '13428'
abstract:
- lang: eng
  text: Mixtures of oppositely charged nanoparticles of various sizes and charge ratios
    precipitate only at the point of electroneutrality. This phenomenonspecific to
    the nanoscale and reminiscent of threshold precipitation of ionsis a consequence
    of the formation of core-and-shell nanoparticle aggregates, in which the shells
    are composed of like-charged particles and are stabilized by efficient electrostatic
    screening.
article_processing_charge: No
article_type: original
author:
- first_name: Alexander M.
  full_name: Kalsin, Alexander M.
  last_name: Kalsin
- first_name: Bartlomiej
  full_name: Kowalczyk, Bartlomiej
  last_name: Kowalczyk
- first_name: Stoyan K.
  full_name: Smoukov, Stoyan K.
  last_name: Smoukov
- first_name: Rafal
  full_name: Klajn, Rafal
  id: 8e84690e-1e48-11ed-a02b-a1e6fb8bb53b
  last_name: Klajn
- first_name: Bartosz A.
  full_name: Grzybowski, Bartosz A.
  last_name: Grzybowski
citation:
  ama: Kalsin AM, Kowalczyk B, Smoukov SK, Klajn R, Grzybowski BA. Ionic-like behavior
    of oppositely charged nanoparticles. <i>Journal of the American Chemical Society</i>.
    2006;128(47):15046-15047. doi:<a href="https://doi.org/10.1021/ja0642966">10.1021/ja0642966</a>
  apa: Kalsin, A. M., Kowalczyk, B., Smoukov, S. K., Klajn, R., &#38; Grzybowski,
    B. A. (2006). Ionic-like behavior of oppositely charged nanoparticles. <i>Journal
    of the American Chemical Society</i>. American Chemical Society. <a href="https://doi.org/10.1021/ja0642966">https://doi.org/10.1021/ja0642966</a>
  chicago: Kalsin, Alexander M., Bartlomiej Kowalczyk, Stoyan K. Smoukov, Rafal Klajn,
    and Bartosz A. Grzybowski. “Ionic-like Behavior of Oppositely Charged Nanoparticles.”
    <i>Journal of the American Chemical Society</i>. American Chemical Society, 2006.
    <a href="https://doi.org/10.1021/ja0642966">https://doi.org/10.1021/ja0642966</a>.
  ieee: A. M. Kalsin, B. Kowalczyk, S. K. Smoukov, R. Klajn, and B. A. Grzybowski,
    “Ionic-like behavior of oppositely charged nanoparticles,” <i>Journal of the American
    Chemical Society</i>, vol. 128, no. 47. American Chemical Society, pp. 15046–15047,
    2006.
  ista: Kalsin AM, Kowalczyk B, Smoukov SK, Klajn R, Grzybowski BA. 2006. Ionic-like
    behavior of oppositely charged nanoparticles. Journal of the American Chemical
    Society. 128(47), 15046–15047.
  mla: Kalsin, Alexander M., et al. “Ionic-like Behavior of Oppositely Charged Nanoparticles.”
    <i>Journal of the American Chemical Society</i>, vol. 128, no. 47, American Chemical
    Society, 2006, pp. 15046–47, doi:<a href="https://doi.org/10.1021/ja0642966">10.1021/ja0642966</a>.
  short: A.M. Kalsin, B. Kowalczyk, S.K. Smoukov, R. Klajn, B.A. Grzybowski, Journal
    of the American Chemical Society 128 (2006) 15046–15047.
date_created: 2023-08-01T10:36:27Z
date_published: 2006-11-29T00:00:00Z
date_updated: 2023-08-08T11:30:06Z
day: '29'
doi: 10.1021/ja0642966
extern: '1'
external_id:
  pmid:
  - '17117829'
intvolume: '       128'
issue: '47'
keyword:
- Colloid and Surface Chemistry
- Biochemistry
- General Chemistry
- Catalysis
language:
- iso: eng
month: '11'
oa_version: None
page: 15046-15047
pmid: 1
publication: Journal of the American Chemical Society
publication_identifier:
  eissn:
  - 1520-5126
  issn:
  - 0002-7863
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ionic-like behavior of oppositely charged nanoparticles
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 128
year: '2006'
...
---
_id: '13429'
abstract:
- lang: eng
  text: 'The fruitful core: Organic syntheses reported in the literature from 1850
    to 2004 are analyzed with mathematical tools from network theory and statistical
    physics. There is a set of substances (the core) from which the majority of other
    organic compounds can be made (see picture; red: core, blue: periphery, green:
    islands). Search algorithms are used to identify small optimal sets of maximally
    useful chemicals.'
article_processing_charge: No
article_type: original
author:
- first_name: Kyle J. M.
  full_name: Bishop, Kyle J. M.
  last_name: Bishop
- first_name: Rafal
  full_name: Klajn, Rafal
  id: 8e84690e-1e48-11ed-a02b-a1e6fb8bb53b
  last_name: Klajn
- first_name: Bartosz A.
  full_name: Grzybowski, Bartosz A.
  last_name: Grzybowski
citation:
  ama: Bishop KJM, Klajn R, Grzybowski BA. The core and most useful molecules in organic
    chemistry. <i>Angewandte Chemie International Edition</i>. 2006;45(32):5348-5354.
    doi:<a href="https://doi.org/10.1002/anie.200600881">10.1002/anie.200600881</a>
  apa: Bishop, K. J. M., Klajn, R., &#38; Grzybowski, B. A. (2006). The core and most
    useful molecules in organic chemistry. <i>Angewandte Chemie International Edition</i>.
    Wiley. <a href="https://doi.org/10.1002/anie.200600881">https://doi.org/10.1002/anie.200600881</a>
  chicago: Bishop, Kyle J. M., Rafal Klajn, and Bartosz A. Grzybowski. “The Core and
    Most Useful Molecules in Organic Chemistry.” <i>Angewandte Chemie International
    Edition</i>. Wiley, 2006. <a href="https://doi.org/10.1002/anie.200600881">https://doi.org/10.1002/anie.200600881</a>.
  ieee: K. J. M. Bishop, R. Klajn, and B. A. Grzybowski, “The core and most useful
    molecules in organic chemistry,” <i>Angewandte Chemie International Edition</i>,
    vol. 45, no. 32. Wiley, pp. 5348–5354, 2006.
  ista: Bishop KJM, Klajn R, Grzybowski BA. 2006. The core and most useful molecules
    in organic chemistry. Angewandte Chemie International Edition. 45(32), 5348–5354.
  mla: Bishop, Kyle J. M., et al. “The Core and Most Useful Molecules in Organic Chemistry.”
    <i>Angewandte Chemie International Edition</i>, vol. 45, no. 32, Wiley, 2006,
    pp. 5348–54, doi:<a href="https://doi.org/10.1002/anie.200600881">10.1002/anie.200600881</a>.
  short: K.J.M. Bishop, R. Klajn, B.A. Grzybowski, Angewandte Chemie International
    Edition 45 (2006) 5348–5354.
date_created: 2023-08-01T10:37:16Z
date_published: 2006-08-11T00:00:00Z
date_updated: 2023-08-08T11:31:27Z
day: '11'
doi: 10.1002/anie.200600881
extern: '1'
external_id:
  pmid:
  - '16835857'
intvolume: '        45'
issue: '32'
keyword:
- General Chemistry
- Catalysis
language:
- iso: eng
month: '08'
oa_version: None
page: 5348-5354
pmid: 1
publication: Angewandte Chemie International Edition
publication_identifier:
  eissn:
  - 1521-3773
  issn:
  - 1433-7851
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: The core and most useful molecules in organic chemistry
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2006'
...
---
_id: '8488'
abstract:
- lang: eng
  text: We demonstrate for different protein samples that three-dimensional HNCO and
    HNCA correlation spectra may be recorded in a few minutes acquisition time using
    the band-selective excitation short-transient sequences presented here. This opens
    new perspectives for the NMR structural investigation of unstable protein samples
    and real-time site-resolved studies of protein kinetics.
article_processing_charge: No
article_type: original
author:
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Hélène
  full_name: Van Melckebeke, Hélène
  last_name: Van Melckebeke
- first_name: Bernhard
  full_name: Brutscher, Bernhard
  last_name: Brutscher
citation:
  ama: Schanda P, Van Melckebeke H, Brutscher B. Speeding up three-dimensional protein
    NMR experiments to a few minutes. <i>Journal of the American Chemical Society</i>.
    2006;128(28):9042-9043. doi:<a href="https://doi.org/10.1021/ja062025p">10.1021/ja062025p</a>
  apa: Schanda, P., Van Melckebeke, H., &#38; Brutscher, B. (2006). Speeding up three-dimensional
    protein NMR experiments to a few minutes. <i>Journal of the American Chemical
    Society</i>. American Chemical Society. <a href="https://doi.org/10.1021/ja062025p">https://doi.org/10.1021/ja062025p</a>
  chicago: Schanda, Paul, Hélène Van Melckebeke, and Bernhard Brutscher. “Speeding
    up Three-Dimensional Protein NMR Experiments to a Few Minutes.” <i>Journal of
    the American Chemical Society</i>. American Chemical Society, 2006. <a href="https://doi.org/10.1021/ja062025p">https://doi.org/10.1021/ja062025p</a>.
  ieee: P. Schanda, H. Van Melckebeke, and B. Brutscher, “Speeding up three-dimensional
    protein NMR experiments to a few minutes,” <i>Journal of the American Chemical
    Society</i>, vol. 128, no. 28. American Chemical Society, pp. 9042–9043, 2006.
  ista: Schanda P, Van Melckebeke H, Brutscher B. 2006. Speeding up three-dimensional
    protein NMR experiments to a few minutes. Journal of the American Chemical Society.
    128(28), 9042–9043.
  mla: Schanda, Paul, et al. “Speeding up Three-Dimensional Protein NMR Experiments
    to a Few Minutes.” <i>Journal of the American Chemical Society</i>, vol. 128,
    no. 28, American Chemical Society, 2006, pp. 9042–43, doi:<a href="https://doi.org/10.1021/ja062025p">10.1021/ja062025p</a>.
  short: P. Schanda, H. Van Melckebeke, B. Brutscher, Journal of the American Chemical
    Society 128 (2006) 9042–9043.
date_created: 2020-09-18T10:13:36Z
date_published: 2006-06-21T00:00:00Z
date_updated: 2021-01-12T08:19:37Z
day: '21'
doi: 10.1021/ja062025p
extern: '1'
intvolume: '       128'
issue: '28'
keyword:
- Colloid and Surface Chemistry
- Biochemistry
- General Chemistry
- Catalysis
language:
- iso: eng
month: '06'
oa_version: None
page: 9042-9043
publication: Journal of the American Chemical Society
publication_identifier:
  issn:
  - 0002-7863
  - 1520-5126
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
status: public
title: Speeding up three-dimensional protein NMR experiments to a few minutes
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 128
year: '2006'
...
---
_id: '8492'
abstract:
- lang: eng
  text: We demonstrate for different protein samples that 2D 1H−15N correlation NMR
    spectra can be recorded in a few seconds of acquisition time using a new band-selective
    optimized flip-angle short-transient heteronuclear multiple quantum coherence
    experiment. This has enabled us to measure fast hydrogen−deuterium exchange rate
    constants along the backbone of a small globular protein fragment by real-time
    2D NMR.
article_processing_charge: No
article_type: original
author:
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
- first_name: Bernhard
  full_name: Brutscher, Bernhard
  last_name: Brutscher
citation:
  ama: Schanda P, Brutscher B. Very fast two-dimensional NMR spectroscopy for real-time
    investigation of dynamic events in proteins on the time scale of seconds. <i>Journal
    of the American Chemical Society</i>. 2005;127(22):8014-8015. doi:<a href="https://doi.org/10.1021/ja051306e">10.1021/ja051306e</a>
  apa: Schanda, P., &#38; Brutscher, B. (2005). Very fast two-dimensional NMR spectroscopy
    for real-time investigation of dynamic events in proteins on the time scale of
    seconds. <i>Journal of the American Chemical Society</i>. American Chemical Society.
    <a href="https://doi.org/10.1021/ja051306e">https://doi.org/10.1021/ja051306e</a>
  chicago: Schanda, Paul, and Bernhard Brutscher. “Very Fast Two-Dimensional NMR Spectroscopy
    for Real-Time Investigation of Dynamic Events in Proteins on the Time Scale of
    Seconds.” <i>Journal of the American Chemical Society</i>. American Chemical Society,
    2005. <a href="https://doi.org/10.1021/ja051306e">https://doi.org/10.1021/ja051306e</a>.
  ieee: P. Schanda and B. Brutscher, “Very fast two-dimensional NMR spectroscopy for
    real-time investigation of dynamic events in proteins on the time scale of seconds,”
    <i>Journal of the American Chemical Society</i>, vol. 127, no. 22. American Chemical
    Society, pp. 8014–8015, 2005.
  ista: Schanda P, Brutscher B. 2005. Very fast two-dimensional NMR spectroscopy for
    real-time investigation of dynamic events in proteins on the time scale of seconds.
    Journal of the American Chemical Society. 127(22), 8014–8015.
  mla: Schanda, Paul, and Bernhard Brutscher. “Very Fast Two-Dimensional NMR Spectroscopy
    for Real-Time Investigation of Dynamic Events in Proteins on the Time Scale of
    Seconds.” <i>Journal of the American Chemical Society</i>, vol. 127, no. 22, American
    Chemical Society, 2005, pp. 8014–15, doi:<a href="https://doi.org/10.1021/ja051306e">10.1021/ja051306e</a>.
  short: P. Schanda, B. Brutscher, Journal of the American Chemical Society 127 (2005)
    8014–8015.
date_created: 2020-09-18T10:14:05Z
date_published: 2005-05-14T00:00:00Z
date_updated: 2021-01-12T08:19:39Z
day: '14'
doi: 10.1021/ja051306e
extern: '1'
intvolume: '       127'
issue: '22'
keyword:
- Colloid and Surface Chemistry
- Biochemistry
- General Chemistry
- Catalysis
language:
- iso: eng
month: '05'
oa_version: None
page: 8014-8015
publication: Journal of the American Chemical Society
publication_identifier:
  issn:
  - 0002-7863
  - 1520-5126
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
status: public
title: Very fast two-dimensional NMR spectroscopy for real-time investigation of dynamic
  events in proteins on the time scale of seconds
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 127
year: '2005'
...