[{"day":"01","date_updated":"2026-07-13T13:14:43Z","type":"journal_article","author":[{"full_name":"Stoev, Iliya D","last_name":"Stoev","first_name":"Iliya D"},{"last_name":"Bolger-Munro","full_name":"Bolger-Munro, Madison","first_name":"Madison","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","orcid":"0000-0002-8176-4824"},{"first_name":"Antonio","last_name":"Minopoli","full_name":"Minopoli, Antonio"},{"first_name":"Susan","last_name":"Wagner","full_name":"Wagner, Susan"},{"first_name":"Venkat Raghavan","last_name":"Krishnaswamy","full_name":"Krishnaswamy, Venkat Raghavan"},{"last_name":"Erben","full_name":"Erben, Elena","first_name":"Elena"},{"full_name":"Weißenbruch, Kai","last_name":"Weißenbruch","first_name":"Kai"},{"first_name":"Nicola","last_name":"Maghelli","full_name":"Maghelli, Nicola"},{"first_name":"Martin","last_name":"Bastmeyer","full_name":"Bastmeyer, Martin"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"},{"first_name":"Moritz","full_name":"Kreysing, Moritz","last_name":"Kreysing"}],"publisher":"Oxford University Press","status":"public","abstract":[{"text":"Determination of the rheological properties of cells is known to require active measurements, which largely depend on the internalization of mechanical probes. Here, we circumvent this problem via the introduction of Rheological focused light-induced cytoplasmic streaming (Rheo-FLUCS): an active, yet probe-free approach that leverages light-induced flows to access mechanical changes in complex systems. While Rheo-FLUCS is facilitated by thermoviscous expansion phenomena rather than external forces, here we show equivalence in its ability to measure relative viscoelastic properties. Specifically, we demonstrate a phase-lag equivalence with probe-dependent active microrheology in a wide range of physically different, yet chemically identical materials. We exemplify the utility of Rheo-FLUCS in three distinctly different biological systems: compound-treated mouse fibroblasts (NIH-3T3), genetically modified human osteoblasts (U2OS) to elucidate the role of myosins in cytoplasmic mechanics, and early ascidian oocytes of Phallusia mammillata at fertilization stage. Our biological use-cases exemplify the application versatility of Rheo-FLUCS, which in the future may use phase information as a marker for developmental success.","lang":"eng"}],"publication":"PNAS Nexus","file":[{"success":1,"checksum":"bb2c89ea73da762ad6423ac299ac3533","date_created":"2026-07-13T13:13:56Z","relation":"main_file","file_size":3073062,"creator":"dernst","access_level":"open_access","file_name":"2026_PNASNexus_Stoev.pdf","file_id":"22312","date_updated":"2026-07-13T13:13:56Z","content_type":"application/pdf"}],"supplementarymaterial":"yes","issue":"6","oa_version":"Published Version","doi":"10.1093/pnasnexus/pgag190","article_processing_charge":"Yes","volume":5,"file_date_updated":"2026-07-13T13:13:56Z","OA_type":"gold","language":[{"iso":"eng"}],"date_published":"2026-06-01T00:00:00Z","OA_place":"publisher","publication_identifier":{"eissn":["2752-6542"]},"biorxivid":1,"article_number":"pgag190","_id":"22297","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","researchdata_availability":"yes","date_created":"2026-07-13T09:50:12Z","quality_controlled":"1","department":[{"_id":"CaHe"}],"title":"Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"PlanS_conform":"1","has_accepted_license":"1","citation":{"short":"I.D. Stoev, M. Bolger-Munro, A. Minopoli, S. Wagner, V.R. Krishnaswamy, E. Erben, K. Weißenbruch, N. Maghelli, M. Bastmeyer, C.-P.J. Heisenberg, M. Kreysing, PNAS Nexus 5 (2026).","ista":"Stoev ID, Bolger-Munro M, Minopoli A, Wagner S, Krishnaswamy VR, Erben E, Weißenbruch K, Maghelli N, Bastmeyer M, Heisenberg C-PJ, Kreysing M. 2026. Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows. PNAS Nexus. 5(6), pgag190.","ama":"Stoev ID, Bolger-Munro M, Minopoli A, et al. Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows. <i>PNAS Nexus</i>. 2026;5(6). doi:<a href=\"https://doi.org/10.1093/pnasnexus/pgag190\">10.1093/pnasnexus/pgag190</a>","apa":"Stoev, I. D., Bolger-Munro, M., Minopoli, A., Wagner, S., Krishnaswamy, V. R., Erben, E., … Kreysing, M. (2026). Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows. <i>PNAS Nexus</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pnasnexus/pgag190\">https://doi.org/10.1093/pnasnexus/pgag190</a>","ieee":"I. D. Stoev <i>et al.</i>, “Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows,” <i>PNAS Nexus</i>, vol. 5, no. 6. Oxford University Press, 2026.","chicago":"Stoev, Iliya D, Madison Bolger-Munro, Antonio Minopoli, Susan Wagner, Venkat Raghavan Krishnaswamy, Elena Erben, Kai Weißenbruch, et al. “Active and Probe-Free Intracellular Rheology via Phase-Sensitive Thermoviscous Flows.” <i>PNAS Nexus</i>. Oxford University Press, 2026. <a href=\"https://doi.org/10.1093/pnasnexus/pgag190\">https://doi.org/10.1093/pnasnexus/pgag190</a>.","mla":"Stoev, Iliya D., et al. “Active and Probe-Free Intracellular Rheology via Phase-Sensitive Thermoviscous Flows.” <i>PNAS Nexus</i>, vol. 5, no. 6, pgag190, Oxford University Press, 2026, doi:<a href=\"https://doi.org/10.1093/pnasnexus/pgag190\">10.1093/pnasnexus/pgag190</a>."},"publication_status":"published","dataavailabilitystatement":"All data and materials are made available on Zenodo public repository under Creative Commons Attribution 4.0 International License: https://doi.org/10.5281/zenodo.20322020.","ddc":["570"],"acknowledgement":"The authors gratefully acknowledge help from Dr. Benjamin Seelbinder, Mr. Claudius George, and Mr. Falk Elsner in designing and constructing the magnetic needle for force-driven rheology experiments. We thank Dr. Iain Patten for valuable discussions on the structure of the manuscript and Mr. Ivan Saraev for graphics support. I.D.S. and M.K. kindly acknowledge funding from the Life grant by Volkswagen Foundation (Life! grant no. 92772), Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, Germany's Excellence Strategy, 2082/1 and grant no. 515462906), and the Hector Foundation. I.D.S. was additionally funded by the Karlsruhe Institute of Technology (Excellence Strategy via the Young Investigator Group Preparation Program). V.R.K., S.W., and M.K. thank the ERC Starting Grant GHOSTs (grant no. 853619).","article_type":"original","oa":1,"scopus_import":"1","month":"06","DOAJ_listed":"1","das_tickbox":"1","intvolume":"         5","keyword":["cell mechanics","active microrheology","noninvasiveness","thermoviscous flows","FLUCS"],"year":"2026","external_id":{"biorxivid":["10.1101/2025.04.07.647540"]}},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"PlanS_conform":"1","citation":{"ieee":"I. Company-Garrido <i>et al.</i>, “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” <i>Developmental Cell</i>, vol. 61, no. 2. Elsevier, p. 356–371.e12, 2026.","chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>.","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>, vol. 61, no. 2, Elsevier, 2026, p. 356–371.e12, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>.","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12.","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12.","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. 2026;61(2):356-371.e12. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>","apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>"},"pmid":1,"has_accepted_license":"1","date_created":"2025-12-28T23:01:27Z","researchdata_availability":"upon request","title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses","quality_controlled":"1","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"_id":"20859","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"OA_place":"publisher","external_id":{"pmid":["41192429"]},"year":"2026","intvolume":"        61","das_tickbox":"1","keyword":["thermobiology","cell migration","thermo-adaptability of immune cells"],"month":"02","acknowledged_ssus":[{"_id":"NanoFab"}],"acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","dataavailabilitystatement":"This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","ddc":["570"],"publication_status":"published","scopus_import":"1","oa":1,"article_type":"original","publisher":"Elsevier","author":[{"first_name":"Iván","last_name":"Company-Garrido","full_name":"Company-Garrido, Iván"},{"last_name":"Zurita Carpio","full_name":"Zurita Carpio, Alberto","first_name":"Alberto"},{"first_name":"Mariona","full_name":"Colomer-Rosell, Mariona","last_name":"Colomer-Rosell"},{"full_name":"Ciraulo, Bernard","last_name":"Ciraulo","first_name":"Bernard"},{"first_name":"Ronja","full_name":"Molkenbur, Ronja","last_name":"Molkenbur"},{"first_name":"Peter","full_name":"Lanzerstorfer, Peter","last_name":"Lanzerstorfer"},{"first_name":"Fabio","last_name":"Pezzano","full_name":"Pezzano, Fabio"},{"first_name":"Costanza","last_name":"Agazzi","full_name":"Agazzi, Costanza"},{"full_name":"Hauschild, Robert","last_name":"Hauschild","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"},{"last_name":"Jain","full_name":"Jain, Saumey","first_name":"Saumey"},{"last_name":"Jacques","full_name":"Jacques, Jeroen M.","first_name":"Jeroen M."},{"first_name":"Valeria","last_name":"Venturini","full_name":"Venturini, Valeria"},{"first_name":"Christian","last_name":"Knapp","full_name":"Knapp, Christian"},{"first_name":"Yufei","full_name":"Xie, Yufei","last_name":"Xie"},{"orcid":"0000-0001-5145-4609","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","last_name":"Merrin"},{"last_name":"Weghuber","full_name":"Weghuber, Julian","first_name":"Julian"},{"last_name":"Schaaf","full_name":"Schaaf, Marcel","first_name":"Marcel"},{"first_name":"Romain","full_name":"Quidant, Romain","last_name":"Quidant"},{"last_name":"Kiermaier","full_name":"Kiermaier, Eva","orcid":"0000-0001-6165-5738","first_name":"Eva","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ortega Arroyo","full_name":"Ortega Arroyo, Jaime","first_name":"Jaime"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","orcid":"0000-0003-4088-8633","full_name":"Ruprecht, Verena","last_name":"Ruprecht"},{"last_name":"Wieser","full_name":"Wieser, Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan","orcid":"0000-0002-2670-2217"}],"type":"journal_article","date_updated":"2026-07-23T06:27:15Z","day":"11","date_published":"2026-02-11T00:00:00Z","language":[{"iso":"eng"}],"OA_type":"hybrid","file_date_updated":"2026-07-23T06:26:25Z","page":"356-371.e12","doi":"10.1016/j.devcel.2025.10.006","article_processing_charge":"Yes (in subscription journal)","volume":61,"oa_version":"Published Version","abstract":[{"text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo.","lang":"eng"}],"status":"public","issue":"2","file":[{"success":1,"relation":"main_file","checksum":"52fd52d2d19a4514f8fcc1b40f420ca2","date_created":"2026-07-23T06:26:25Z","file_size":12342817,"creator":"dernst","date_updated":"2026-07-23T06:26:25Z","file_id":"22388","file_name":"2026_DevelopmentalCell_CompanyGarrido.pdf","access_level":"open_access","content_type":"application/pdf"}],"supplementarymaterial":"yes","publication":"Developmental Cell"},{"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Autocrine signaling pathways regulated by RAPID ALKALINIZATION FACTORs (RALFs) control cell wall integrity during pollen tube germination and growth in Arabidopsis (Arabidopsis thaliana). To investigate the role of pollen-specific RALFs in another plant species, we combined gene expression data with phylogenetic and biochemical studies to identify candidate orthologs in maize (Zea mays). We show that Clade IB ZmRALF2/3 mutations, but not Clade III ZmRALF1/5 mutations, cause cell wall instability in the sub-apical region of the growing pollen tube. ZmRALF2/3 are mainly located in the cell wall and are partially able to complement the pollen germination defect of their Arabidopsis orthologs AtRALF4/19. Mutations in ZmRALF2/3 compromise pectin distribution patterns leading to altered cell wall organization and thickness culminating in pollen tube burst. Clade IB, but not Clade III ZmRALFs, strongly interact as ligands with the pollen-specific Catharanthus roseus RLK1-like (CrRLK1L) receptor kinases Zea mays FERONIA-like (ZmFERL) 4/7/9, LORELEI-like glycosylphosphatidylinositol-anchor (LLG) proteins Zea mays LLG 1 and 2 (ZmLLG1/2) and Zea mays pollen extension-like (PEX) cell wall proteins ZmPEX2/4. Notably, ZmFERL4 outcompetes ZmLLG2 and ZmPEX2 outcompetes ZmFERL4 for ZmRALF2 binding. Based on these data, we suggest that Clade IB RALFs act in a dual role as cell wall components and extracellular sensors to regulate cell wall integrity and thickness during pollen tube growth in maize and probably other plants."}],"status":"public","issue":"5","publication":"The Plant Cell","date_published":"2024-05-01T00:00:00Z","language":[{"iso":"eng"}],"doi":"10.1093/plcell/koad324","article_processing_charge":"No","volume":36,"date_updated":"2024-07-16T11:18:46Z","day":"01","publisher":"Oxford University Press","author":[{"first_name":"Liang-Zi","last_name":"Zhou","full_name":"Zhou, Liang-Zi"},{"first_name":"Lele","full_name":"Wang, Lele","last_name":"Wang"},{"first_name":"Xia","full_name":"Chen, Xia","last_name":"Chen"},{"full_name":"Ge, Zengxiang","last_name":"Ge","orcid":"0000-0001-9381-3577","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","first_name":"Zengxiang"},{"full_name":"Mergner, Julia","last_name":"Mergner","first_name":"Julia"},{"first_name":"Xingli","last_name":"Li","full_name":"Li, Xingli"},{"first_name":"Bernhard","full_name":"Küster, Bernhard","last_name":"Küster"},{"last_name":"Längst","full_name":"Längst, Gernot","first_name":"Gernot"},{"first_name":"Li-Jia","full_name":"Qu, Li-Jia","last_name":"Qu"},{"last_name":"Dresselhaus","full_name":"Dresselhaus, Thomas","first_name":"Thomas"}],"type":"journal_article","month":"05","ddc":["580"],"publication_status":"published","oa":1,"article_type":"original","scopus_import":"1","external_id":{"pmid":["38142229"]},"year":"2024","intvolume":"        36","keyword":["Cell Biology","Plant Science"],"_id":"14726","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"article_number":"koad324","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"citation":{"chicago":"Zhou, Liang-Zi, Lele Wang, Xia Chen, Zengxiang Ge, Julia Mergner, Xingli Li, Bernhard Küster, Gernot Längst, Li-Jia Qu, and Thomas Dresselhaus. “The RALF Signaling Pathway Regulates Cell Wall Integrity during Pollen Tube Growth in Maize.” <i>The Plant Cell</i>. Oxford University Press, 2024. <a href=\"https://doi.org/10.1093/plcell/koad324\">https://doi.org/10.1093/plcell/koad324</a>.","ieee":"L.-Z. Zhou <i>et al.</i>, “The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize,” <i>The Plant Cell</i>, vol. 36, no. 5. Oxford University Press, 2024.","mla":"Zhou, Liang-Zi, et al. “The RALF Signaling Pathway Regulates Cell Wall Integrity during Pollen Tube Growth in Maize.” <i>The Plant Cell</i>, vol. 36, no. 5, koad324, Oxford University Press, 2024, doi:<a href=\"https://doi.org/10.1093/plcell/koad324\">10.1093/plcell/koad324</a>.","ista":"Zhou L-Z, Wang L, Chen X, Ge Z, Mergner J, Li X, Küster B, Längst G, Qu L-J, Dresselhaus T. 2024. The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. The Plant Cell. 36(5), koad324.","short":"L.-Z. Zhou, L. Wang, X. Chen, Z. Ge, J. Mergner, X. Li, B. Küster, G. Längst, L.-J. Qu, T. Dresselhaus, The Plant Cell 36 (2024).","ama":"Zhou L-Z, Wang L, Chen X, et al. The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. <i>The Plant Cell</i>. 2024;36(5). doi:<a href=\"https://doi.org/10.1093/plcell/koad324\">10.1093/plcell/koad324</a>","apa":"Zhou, L.-Z., Wang, L., Chen, X., Ge, Z., Mergner, J., Li, X., … Dresselhaus, T. (2024). The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koad324\">https://doi.org/10.1093/plcell/koad324</a>"},"pmid":1,"has_accepted_license":"1","date_created":"2024-01-02T11:19:37Z","main_file_link":[{"url":"https://doi.org/10.1093/plcell/koad324","open_access":"1"}],"extern":"1","title":"The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize","quality_controlled":"1"},{"volume":103,"article_processing_charge":"Yes","doi":"10.1016/j.ejcb.2023.151380","language":[{"iso":"eng"}],"date_published":"2024-03-01T00:00:00Z","file_date_updated":"2024-07-16T12:07:20Z","issue":"1","file":[{"content_type":"application/pdf","access_level":"open_access","file_id":"17265","date_updated":"2024-07-16T12:07:20Z","file_name":"2024_EJCB_Radler.pdf","creator":"dernst","file_size":9995304,"checksum":"5d170abbc87585205c010657e4552360","date_created":"2024-07-16T12:07:20Z","relation":"main_file","success":1}],"publication":"European Journal of Cell Biology","corr_author":"1","abstract":[{"text":"Bacteria divide by binary fission. The protein machine responsible for this process is the divisome, a transient assembly of more than 30 proteins in and on the surface of the cytoplasmic membrane. Together, they constrict the cell envelope and remodel the peptidoglycan layer to eventually split the cell into two. For Escherichia coli, most molecular players involved in this process have probably been identified, but obtaining the quantitative information needed for a mechanistic understanding can often not be achieved from experiments in vivo alone. Since the discovery of the Z-ring more than 30 years ago, in vitro reconstitution experiments have been crucial to shed light on molecular processes normally hidden in the complex environment of the living cell. In this review, we summarize how rebuilding the divisome from purified components – or at least parts of it - have been instrumental to obtain the detailed mechanistic understanding of the bacterial cell division machinery that we have today.","lang":"eng"}],"status":"public","oa_version":"Published Version","type":"journal_article","publisher":"Elsevier","author":[{"full_name":"Radler, Philipp","last_name":"Radler","orcid":"0000-0001-9198-2182 ","first_name":"Philipp","id":"40136C2A-F248-11E8-B48F-1D18A9856A87"},{"id":"462D4284-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","orcid":"0000-0001-7309-9724","full_name":"Loose, Martin","last_name":"Loose"}],"day":"01","project":[{"_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d","grant_number":"P34607","name":"In vitro reconstitution of bacterial cell division"}],"isi":1,"date_updated":"2025-09-04T11:45:31Z","keyword":["Cell Biology","General Medicine","Histology","Pathology and Forensic Medicine"],"intvolume":"       103","external_id":{"pmid":["38218128"],"isi":["001166216800001"]},"year":"2024","oa":1,"scopus_import":"1","article_type":"review","publication_status":"published","ddc":["570"],"acknowledgement":"We acknowledge members of the Loose laboratory at ISTA for helpful discussions—in particular M. Kojic for his insightful comments. This work was supported by the Austrian Science Fund (FWF P34607) to M.L.","month":"03","title":"A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches","quality_controlled":"1","department":[{"_id":"MaLo"}],"date_created":"2024-01-18T08:16:43Z","pmid":1,"citation":{"ista":"Radler P, Loose M. 2024. A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. European Journal of Cell Biology. 103(1), 151380.","short":"P. Radler, M. Loose, European Journal of Cell Biology 103 (2024).","ama":"Radler P, Loose M. A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. <i>European Journal of Cell Biology</i>. 2024;103(1). doi:<a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">10.1016/j.ejcb.2023.151380</a>","apa":"Radler, P., &#38; Loose, M. (2024). A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches. <i>European Journal of Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">https://doi.org/10.1016/j.ejcb.2023.151380</a>","chicago":"Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.” <i>European Journal of Cell Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">https://doi.org/10.1016/j.ejcb.2023.151380</a>.","ieee":"P. Radler and M. Loose, “A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches,” <i>European Journal of Cell Biology</i>, vol. 103, no. 1. Elsevier, 2024.","mla":"Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.” <i>European Journal of Cell Biology</i>, vol. 103, no. 1, 151380, Elsevier, 2024, doi:<a href=\"https://doi.org/10.1016/j.ejcb.2023.151380\">10.1016/j.ejcb.2023.151380</a>."},"has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_number":"151380","publication_identifier":{"issn":["0171-9335"]},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"14834"},{"date_updated":"2025-04-14T07:52:27Z","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"isi":1,"day":"02","publisher":"Elsevier","author":[{"last_name":"Corominas-Murtra","full_name":"Corominas-Murtra, Bernat","first_name":"Bernat","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9806-5643"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","full_name":"Hannezo, Edouard B"}],"type":"journal_article","oa_version":"Published Version","status":"public","abstract":[{"text":"Homeostatic balance in the intestinal epithelium relies on a fast cellular turnover, which is coordinated by an intricate interplay between biochemical signalling, mechanical forces and organ geometry. We review recent modelling approaches that have been developed to understand different facets of this remarkable homeostatic equilibrium. Existing models offer different, albeit complementary, perspectives on the problem. First, biomechanical models aim to explain the local and global mechanical stresses driving cell renewal as well as tissue shape maintenance. Second, compartmental models provide insights into the conditions necessary to keep a constant flow of cells with well-defined ratios of cell types, and how perturbations can lead to an unbalance of relative compartment sizes. A third family of models address, at the cellular level, the nature and regulation of stem fate choices that are necessary to fuel cellular turnover. We also review how these different approaches are starting to be integrated together across scales, to provide quantitative predictions and new conceptual frameworks to think about the dynamics of cell renewal in complex tissues.","lang":"eng"}],"corr_author":"1","publication":"Seminars in Cell & Developmental Biology","file":[{"relation":"main_file","date_created":"2024-01-08T10:16:04Z","checksum":"c619887cf130f4649bf3035417186004","success":1,"content_type":"application/pdf","creator":"dernst","file_size":1343750,"date_updated":"2024-01-08T10:16:04Z","file_id":"14741","file_name":"2023_SeminarsCellDevBiology_CorominasMurtra.pdf","access_level":"open_access"}],"file_date_updated":"2024-01-08T10:16:04Z","date_published":"2023-12-02T00:00:00Z","language":[{"iso":"eng"}],"doi":"10.1016/j.semcdb.2022.11.005","article_processing_charge":"Yes (via OA deal)","page":"58-65","volume":"150-151","_id":"12162","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["1084-9521"]},"ec_funded":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"has_accepted_license":"1","pmid":1,"citation":{"mla":"Corominas-Murtra, Bernat, and Edouard B. Hannezo. “Modelling the Dynamics of Mammalian Gut Homeostasis.” <i>Seminars in Cell &#38; Developmental Biology</i>, vol. 150–151, Elsevier, 2023, pp. 58–65, doi:<a href=\"https://doi.org/10.1016/j.semcdb.2022.11.005\">10.1016/j.semcdb.2022.11.005</a>.","ieee":"B. Corominas-Murtra and E. B. Hannezo, “Modelling the dynamics of mammalian gut homeostasis,” <i>Seminars in Cell &#38; Developmental Biology</i>, vol. 150–151. Elsevier, pp. 58–65, 2023.","chicago":"Corominas-Murtra, Bernat, and Edouard B Hannezo. “Modelling the Dynamics of Mammalian Gut Homeostasis.” <i>Seminars in Cell &#38; Developmental Biology</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.semcdb.2022.11.005\">https://doi.org/10.1016/j.semcdb.2022.11.005</a>.","ama":"Corominas-Murtra B, Hannezo EB. Modelling the dynamics of mammalian gut homeostasis. <i>Seminars in Cell &#38; Developmental Biology</i>. 2023;150-151:58-65. doi:<a href=\"https://doi.org/10.1016/j.semcdb.2022.11.005\">10.1016/j.semcdb.2022.11.005</a>","apa":"Corominas-Murtra, B., &#38; Hannezo, E. B. (2023). Modelling the dynamics of mammalian gut homeostasis. <i>Seminars in Cell &#38; Developmental Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.semcdb.2022.11.005\">https://doi.org/10.1016/j.semcdb.2022.11.005</a>","short":"B. Corominas-Murtra, E.B. Hannezo, Seminars in Cell &#38; Developmental Biology 150–151 (2023) 58–65.","ista":"Corominas-Murtra B, Hannezo EB. 2023. Modelling the dynamics of mammalian gut homeostasis. Seminars in Cell &#38; Developmental Biology. 150–151, 58–65."},"date_created":"2023-01-12T12:09:47Z","quality_controlled":"1","department":[{"_id":"EdHa"}],"title":"Modelling the dynamics of mammalian gut homeostasis","month":"12","publication_status":"published","acknowledgement":"This work received funding from the ERC under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 851288 to E.H.).\r\nB. C-M wants to acknowledge the support of the field of excellence Complexity of Life, in Basic Research and Innovation of the University of Graz.","ddc":["570"],"article_type":"review","oa":1,"scopus_import":"1","year":"2023","external_id":{"isi":["001053522200001"],"pmid":["36470715"]},"keyword":["Cell Biology","Developmental Biology"]},{"publisher":"Wiley","author":[{"orcid":"0000-0001-7309-9724","first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","full_name":"Loose, Martin","last_name":"Loose"},{"orcid":"0000-0002-3580-2906","first_name":"Albert","id":"3018E8C2-F248-11E8-B48F-1D18A9856A87","last_name":"Auer","full_name":"Auer, Albert"},{"last_name":"Brognara","full_name":"Brognara, Gabriel","id":"D96FFDA0-A884-11E9-9968-DC26E6697425","first_name":"Gabriel"},{"first_name":"Hanifatul R","id":"55380f95-15b2-11ec-abd3-aff8e230696b","full_name":"Budiman, Hanifatul R","last_name":"Budiman"},{"id":"e3a512e2-4bbe-11eb-a68a-e3857a7844c2","first_name":"Lukasz M","last_name":"Kowalski","full_name":"Kowalski, Lukasz M"},{"last_name":"Matijevic","full_name":"Matijevic, Ivana","id":"83c17ce3-15b2-11ec-abd3-f486545870bd","first_name":"Ivana"}],"type":"journal_article","date_updated":"2024-10-09T21:03:42Z","isi":1,"day":"01","file_date_updated":"2023-08-16T08:31:04Z","date_published":"2023-03-01T00:00:00Z","language":[{"iso":"eng"}],"article_processing_charge":"Yes (via OA deal)","doi":"10.1002/1873-3468.14540","page":"762-777","volume":597,"oa_version":"Published Version","status":"public","abstract":[{"text":"Small GTPases play essential roles in the organization of eukaryotic cells. In recent years, it has become clear that their intracellular functions result from intricate biochemical networks of the GTPase and their regulators that dynamically bind to a membrane surface. Due to the inherent complexities of their interactions, however, revealing the underlying mechanisms of action is often difficult to achieve from in vivo studies. This review summarizes in vitro reconstitution approaches developed to obtain a better mechanistic understanding of how small GTPase activities are regulated in space and time.","lang":"eng"}],"corr_author":"1","file":[{"content_type":"application/pdf","creator":"dernst","file_size":3148143,"access_level":"open_access","file_id":"14063","date_updated":"2023-08-16T08:31:04Z","file_name":"2023_FEBSLetters_Loose.pdf","date_created":"2023-08-16T08:31:04Z","checksum":"7492244d3f9c5faa1347ef03f6e5bc84","relation":"main_file","success":1}],"publication":"FEBS Letters","issue":"6","tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"has_accepted_license":"1","citation":{"chicago":"Loose, Martin, Albert Auer, Gabriel Brognara, Hanifatul R Budiman, Lukasz M Kowalski, and Ivana Matijevic. “In Vitro Reconstitution of Small GTPase Regulation.” <i>FEBS Letters</i>. Wiley, 2023. <a href=\"https://doi.org/10.1002/1873-3468.14540\">https://doi.org/10.1002/1873-3468.14540</a>.","ieee":"M. Loose, A. Auer, G. Brognara, H. R. Budiman, L. M. Kowalski, and I. Matijevic, “In vitro reconstitution of small GTPase regulation,” <i>FEBS Letters</i>, vol. 597, no. 6. Wiley, pp. 762–777, 2023.","mla":"Loose, Martin, et al. “In Vitro Reconstitution of Small GTPase Regulation.” <i>FEBS Letters</i>, vol. 597, no. 6, Wiley, 2023, pp. 762–77, doi:<a href=\"https://doi.org/10.1002/1873-3468.14540\">10.1002/1873-3468.14540</a>.","ista":"Loose M, Auer A, Brognara G, Budiman HR, Kowalski LM, Matijevic I. 2023. In vitro reconstitution of small GTPase regulation. FEBS Letters. 597(6), 762–777.","short":"M. Loose, A. Auer, G. Brognara, H.R. Budiman, L.M. Kowalski, I. Matijevic, FEBS Letters 597 (2023) 762–777.","apa":"Loose, M., Auer, A., Brognara, G., Budiman, H. R., Kowalski, L. M., &#38; Matijevic, I. (2023). In vitro reconstitution of small GTPase regulation. <i>FEBS Letters</i>. Wiley. <a href=\"https://doi.org/10.1002/1873-3468.14540\">https://doi.org/10.1002/1873-3468.14540</a>","ama":"Loose M, Auer A, Brognara G, Budiman HR, Kowalski LM, Matijevic I. In vitro reconstitution of small GTPase regulation. <i>FEBS Letters</i>. 2023;597(6):762-777. doi:<a href=\"https://doi.org/10.1002/1873-3468.14540\">10.1002/1873-3468.14540</a>"},"pmid":1,"date_created":"2023-01-12T12:09:58Z","department":[{"_id":"MaLo"}],"quality_controlled":"1","title":"In vitro reconstitution of small GTPase regulation","_id":"12163","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["0014-5793"],"eissn":["1873-3468"]},"year":"2023","external_id":{"isi":["000891573000001"],"pmid":["36448231"]},"intvolume":"       597","keyword":["Cell Biology","Genetics","Molecular Biology","Biochemistry","Structural Biology","Biophysics"],"month":"03","ddc":["570"],"acknowledgement":"The authors acknowledge support from IST Austria and helpful comments from the anonymous reviewers that helped to improve this manuscript. We apologize to the authors of primary literature and outstanding research not cited here due to space restraints.","publication_status":"published","scopus_import":"1","article_type":"review","oa":1},{"_id":"12669","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"article_number":"koac346","pmid":1,"citation":{"ieee":"P. A. Manavella <i>et al.</i>, “Beyond transcription: compelling open questions in plant RNA biology,” <i>The Plant Cell</i>, vol. 35, no. 6. Oxford University Press, 2023.","chicago":"Manavella, Pablo A, Micaela A Godoy Herz, Alberto R Kornblihtt, Reed Sorenson, Leslie E Sieburth, Kentaro Nakaminami, Motoaki Seki, et al. “Beyond Transcription: Compelling Open Questions in Plant RNA Biology.” <i>The Plant Cell</i>. Oxford University Press, 2023. <a href=\"https://doi.org/10.1093/plcell/koac346\">https://doi.org/10.1093/plcell/koac346</a>.","mla":"Manavella, Pablo A., et al. “Beyond Transcription: Compelling Open Questions in Plant RNA Biology.” <i>The Plant Cell</i>, vol. 35, no. 6, koac346, Oxford University Press, 2023, doi:<a href=\"https://doi.org/10.1093/plcell/koac346\">10.1093/plcell/koac346</a>.","short":"P.A. Manavella, M.A. Godoy Herz, A.R. Kornblihtt, R. Sorenson, L.E. Sieburth, K. Nakaminami, M. Seki, Y. Ding, Q. Sun, H. Kang, F.D. Ariel, M. Crespi, A.J. Giudicatti, Q. Cai, H. Jin, X. Feng, Y. Qi, C.S. Pikaard, The Plant Cell 35 (2023).","ista":"Manavella PA, Godoy Herz MA, Kornblihtt AR, Sorenson R, Sieburth LE, Nakaminami K, Seki M, Ding Y, Sun Q, Kang H, Ariel FD, Crespi M, Giudicatti AJ, Cai Q, Jin H, Feng X, Qi Y, Pikaard CS. 2023. Beyond transcription: compelling open questions in plant RNA biology. The Plant Cell. 35(6), koac346.","apa":"Manavella, P. A., Godoy Herz, M. A., Kornblihtt, A. R., Sorenson, R., Sieburth, L. E., Nakaminami, K., … Pikaard, C. S. (2023). Beyond transcription: compelling open questions in plant RNA biology. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koac346\">https://doi.org/10.1093/plcell/koac346</a>","ama":"Manavella PA, Godoy Herz MA, Kornblihtt AR, et al. Beyond transcription: compelling open questions in plant RNA biology. <i>The Plant Cell</i>. 2023;35(6). doi:<a href=\"https://doi.org/10.1093/plcell/koac346\">10.1093/plcell/koac346</a>"},"extern":"1","main_file_link":[{"url":"https://doi.org/10.1093/plcell/koac346","open_access":"1"}],"date_created":"2023-02-23T09:14:59Z","quality_controlled":"1","department":[{"_id":"XiFe"}],"title":"Beyond transcription: compelling open questions in plant RNA biology","month":"06","publication_status":"published","oa":1,"article_type":"original","scopus_import":"1","year":"2023","external_id":{"pmid":["36477566"]},"intvolume":"        35","keyword":["Cell Biology","Plant Science"],"date_updated":"2023-10-04T09:48:43Z","day":"01","author":[{"last_name":"Manavella","full_name":"Manavella, Pablo A","first_name":"Pablo A"},{"first_name":"Micaela A","full_name":"Godoy Herz, Micaela A","last_name":"Godoy Herz"},{"last_name":"Kornblihtt","full_name":"Kornblihtt, Alberto R","first_name":"Alberto R"},{"last_name":"Sorenson","full_name":"Sorenson, Reed","first_name":"Reed"},{"last_name":"Sieburth","full_name":"Sieburth, Leslie E","first_name":"Leslie E"},{"full_name":"Nakaminami, Kentaro","last_name":"Nakaminami","first_name":"Kentaro"},{"first_name":"Motoaki","full_name":"Seki, Motoaki","last_name":"Seki"},{"first_name":"Yiliang","full_name":"Ding, Yiliang","last_name":"Ding"},{"full_name":"Sun, Qianwen","last_name":"Sun","first_name":"Qianwen"},{"last_name":"Kang","full_name":"Kang, Hunseung","first_name":"Hunseung"},{"last_name":"Ariel","full_name":"Ariel, Federico D","first_name":"Federico D"},{"full_name":"Crespi, Martin","last_name":"Crespi","first_name":"Martin"},{"first_name":"Axel J","last_name":"Giudicatti","full_name":"Giudicatti, Axel J"},{"last_name":"Cai","full_name":"Cai, Qiang","first_name":"Qiang"},{"first_name":"Hailing","full_name":"Jin, Hailing","last_name":"Jin"},{"orcid":"0000-0002-4008-1234","first_name":"Xiaoqi","id":"e0164712-22ee-11ed-b12a-d80fcdf35958","full_name":"Feng, Xiaoqi","last_name":"Feng"},{"full_name":"Qi, Yijun","last_name":"Qi","first_name":"Yijun"},{"first_name":"Craig S","full_name":"Pikaard, Craig S","last_name":"Pikaard"}],"publisher":"Oxford University Press","type":"journal_article","oa_version":"Published Version","status":"public","abstract":[{"lang":"eng","text":"The study of RNAs has become one of the most influential research fields in contemporary biology and biomedicine. In the last few years, new sequencing technologies have produced an explosion of new and exciting discoveries in the field but have also given rise to many open questions. Defining these questions, together with old, long-standing gaps in our knowledge, is the spirit of this article. The breadth of topics within RNA biology research is vast, and every aspect of the biology of these molecules contains countless exciting open questions. Here, we asked 12 groups to discuss their most compelling question among some plant RNA biology topics. The following vignettes cover RNA alternative splicing; RNA dynamics; RNA translation; RNA structures; R-loops; epitranscriptomics; long non-coding RNAs; small RNA production and their functions in crops; small RNAs during gametogenesis and in cross-kingdom RNA interference; and RNA-directed DNA methylation. In each section, we will present the current state-of-the-art in plant RNA biology research before asking the questions that will surely motivate future discoveries in the field. We hope this article will spark a debate about the future perspective on RNA biology and provoke novel reflections in the reader."}],"publication":"The Plant Cell","issue":"6","date_published":"2023-06-01T00:00:00Z","language":[{"iso":"eng"}],"article_processing_charge":"No","doi":"10.1093/plcell/koac346","volume":35},{"language":[{"iso":"eng"}],"date_published":"2023-03-20T00:00:00Z","volume":5,"page":"495-515","doi":"10.1038/s42255-023-00766-2","article_processing_charge":"No","oa_version":"Preprint","publication":"Nature Metabolism","abstract":[{"text":"Muscle degeneration is the most prevalent cause for frailty and dependency in inherited diseases and ageing. Elucidation of pathophysiological mechanisms, as well as effective treatments for muscle diseases, represents an important goal in improving human health. Here, we show that the lipid synthesis enzyme phosphatidylethanolamine cytidyltransferase (PCYT2/ECT) is critical to muscle health. Human deficiency in PCYT2 causes a severe disease with failure to thrive and progressive weakness. pcyt2-mutant zebrafish and muscle-specific Pcyt2-knockout mice recapitulate the participant phenotypes, with failure to thrive, progressive muscle weakness and accelerated ageing. Mechanistically, muscle Pcyt2 deficiency affects cellular bioenergetics and membrane lipid bilayer structure and stability. PCYT2 activity declines in ageing muscles of mice and humans, and adeno-associated virus-based delivery of PCYT2 ameliorates muscle weakness in Pcyt2-knockout and old mice, offering a therapy for individuals with a rare disease and muscle ageing. Thus, PCYT2 plays a fundamental and conserved role in vertebrate muscle health, linking PCYT2 and PCYT2-synthesized lipids to severe muscle dystrophy and ageing.","lang":"eng"}],"status":"public","author":[{"first_name":"Domagoj","last_name":"Cikes","full_name":"Cikes, Domagoj"},{"full_name":"Elsayad, Kareem","last_name":"Elsayad","first_name":"Kareem"},{"last_name":"Sezgin","full_name":"Sezgin, Erdinc","first_name":"Erdinc"},{"last_name":"Koitai","full_name":"Koitai, Erika","first_name":"Erika"},{"last_name":"Ferenc","full_name":"Ferenc, Torma","first_name":"Torma"},{"last_name":"Orthofer","full_name":"Orthofer, Michael","first_name":"Michael"},{"first_name":"Rebecca","full_name":"Yarwood, Rebecca","last_name":"Yarwood"},{"last_name":"Heinz","full_name":"Heinz, Leonhard X.","first_name":"Leonhard X."},{"first_name":"Vitaly","full_name":"Sedlyarov, Vitaly","last_name":"Sedlyarov"},{"first_name":"Nasser","id":"39CD9926-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8821-8236","full_name":"Darwish-Miranda, Nasser","last_name":"Darwish-Miranda"},{"full_name":"Taylor, Adrian","last_name":"Taylor","first_name":"Adrian"},{"first_name":"Sophie","last_name":"Grapentine","full_name":"Grapentine, Sophie"},{"first_name":"Fathiya","full_name":"al-Murshedi, Fathiya","last_name":"al-Murshedi"},{"first_name":"Anne","last_name":"Abot","full_name":"Abot, Anne"},{"last_name":"Weidinger","full_name":"Weidinger, Adelheid","first_name":"Adelheid"},{"full_name":"Kutchukian, Candice","last_name":"Kutchukian","first_name":"Candice"},{"full_name":"Sanchez, Colline","last_name":"Sanchez","first_name":"Colline"},{"last_name":"Cronin","full_name":"Cronin, Shane J. F.","first_name":"Shane J. F."},{"last_name":"Novatchkova","full_name":"Novatchkova, Maria","first_name":"Maria"},{"first_name":"Anoop","full_name":"Kavirayani, Anoop","last_name":"Kavirayani"},{"last_name":"Schuetz","full_name":"Schuetz, Thomas","first_name":"Thomas"},{"full_name":"Haubner, Bernhard","last_name":"Haubner","first_name":"Bernhard"},{"first_name":"Lisa","full_name":"Haas, Lisa","last_name":"Haas"},{"last_name":"Hagelkruys","full_name":"Hagelkruys, Astrid","first_name":"Astrid"},{"first_name":"Suzanne","last_name":"Jackowski","full_name":"Jackowski, Suzanne"},{"full_name":"Kozlov, Andrey","last_name":"Kozlov","first_name":"Andrey"},{"first_name":"Vincent","last_name":"Jacquemond","full_name":"Jacquemond, Vincent"},{"full_name":"Knauf, Claude","last_name":"Knauf","first_name":"Claude"},{"first_name":"Giulio","full_name":"Superti-Furga, Giulio","last_name":"Superti-Furga"},{"first_name":"Eric","last_name":"Rullman","full_name":"Rullman, Eric"},{"full_name":"Gustafsson, Thomas","last_name":"Gustafsson","first_name":"Thomas"},{"first_name":"John","full_name":"McDermot, John","last_name":"McDermot"},{"last_name":"Lowe","full_name":"Lowe, Martin","first_name":"Martin"},{"full_name":"Radak, Zsolt","last_name":"Radak","first_name":"Zsolt"},{"first_name":"Jeffrey S.","last_name":"Chamberlain","full_name":"Chamberlain, Jeffrey S."},{"first_name":"Marica","last_name":"Bakovic","full_name":"Bakovic, Marica"},{"last_name":"Banka","full_name":"Banka, Siddharth","first_name":"Siddharth"},{"last_name":"Penninger","full_name":"Penninger, Josef M.","first_name":"Josef M."}],"publisher":"Springer Nature","type":"journal_article","date_updated":"2023-11-28T07:31:33Z","day":"20","isi":1,"external_id":{"isi":["000992064000002"],"pmid":["36941451"]},"year":"2023","keyword":["Cell Biology","Physiology (medical)","Endocrinology","Diabetes and Metabolism","Internal Medicine"],"related_material":{"link":[{"url":"https://doi.org/10.1038/s42255-023-00791-1","relation":"erratum"}]},"intvolume":"         5","month":"03","oa":1,"scopus_import":"1","article_type":"original","publication_status":"published","acknowledgement":"The authors thank the participants and their families for participating in the study. We thank all members of our laboratories for helpful discussions. We are grateful to Vienna BioCenter Core Facilities: Mouse Phenotyping Unit, Histopathology Unit, Bioinformatics Unit, BioOptics Unit, Electron Microscopy Unit and Comparative Medicine Unit. We are grateful to the Lipidomics Facility, and K. Klavins and T. Hannich at the CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences for assistance with lipidomics analysis. We also thank T. Huan and A. Hui (UBC Vancouver) for mouse tissue and mitochondria lipidomics analysis. We thank A. Klymchenko (Laboratoire de Bioimagerie et Pathologies Université de Strasbourg, Strasbourg, France) for providing the NR12S probe. We are thankful to the Sen. Paul D. Wellstone Muscular Dystrophy Cooperative Specialized Research Center Viral Vector Core Facility for AAV6 production. We also thank K. P. Campbell and M. E. Anderson (University of Iowa, Carver College of Medicine) for advice on muscle tissue handling. We thank A. Al-Qassabi from the Sultan Qaboos University for the clinical assessment of the participants. D.C. and J.M.P. are supported by the Austrian Federal Ministry of Education, Science and Research, the Austrian Academy of Sciences, and the City of Vienna, and grants from the Austrian Science Fund (FWF) Wittgenstein award (Z 271-B19), the T. von Zastrow Foundation, and a Canada 150 Research Chairs Program (F18-01336). J.S.C. is supported by grants RO1AR44533 and P50AR065139 from the US National Institutes of Health. C.K. is supported by a grant from the Agence Nationale de la Recherche (ANR-18-CE14-0007-01). A.V.K. is supported by European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 67544, and an Austrian Science Fund (FWF; no P-33799). A.W. is supported by Austrian Research Promotion Agency (FFG) project no 867674. E.S. is supported by a SciLifeLab fellowship and Karolinska Institutet Foundation Grants. Work in the laboratory of G.S.-F. is supported by the Austrian Academy of Sciences, the European Research Council (ERC AdG 695214 GameofGates) and the Innovative Medicines Initiative 2 Joint Undertaking (grant agreement no. 777372, ReSOLUTE). S.B., M.L. and R.Y. acknowledge the support of the Spastic Paraplegia Foundation.","citation":{"mla":"Cikes, Domagoj, et al. “PCYT2-Regulated Lipid Biosynthesis Is Critical to Muscle Health and Ageing.” <i>Nature Metabolism</i>, vol. 5, Springer Nature, 2023, pp. 495–515, doi:<a href=\"https://doi.org/10.1038/s42255-023-00766-2\">10.1038/s42255-023-00766-2</a>.","ieee":"D. Cikes <i>et al.</i>, “PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing,” <i>Nature Metabolism</i>, vol. 5. Springer Nature, pp. 495–515, 2023.","chicago":"Cikes, Domagoj, Kareem Elsayad, Erdinc Sezgin, Erika Koitai, Torma Ferenc, Michael Orthofer, Rebecca Yarwood, et al. “PCYT2-Regulated Lipid Biosynthesis Is Critical to Muscle Health and Ageing.” <i>Nature Metabolism</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s42255-023-00766-2\">https://doi.org/10.1038/s42255-023-00766-2</a>.","ama":"Cikes D, Elsayad K, Sezgin E, et al. PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing. <i>Nature Metabolism</i>. 2023;5:495-515. doi:<a href=\"https://doi.org/10.1038/s42255-023-00766-2\">10.1038/s42255-023-00766-2</a>","apa":"Cikes, D., Elsayad, K., Sezgin, E., Koitai, E., Ferenc, T., Orthofer, M., … Penninger, J. M. (2023). PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing. <i>Nature Metabolism</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42255-023-00766-2\">https://doi.org/10.1038/s42255-023-00766-2</a>","short":"D. Cikes, K. Elsayad, E. Sezgin, E. Koitai, T. Ferenc, M. Orthofer, R. Yarwood, L.X. Heinz, V. Sedlyarov, N. Darwish-Miranda, A. Taylor, S. Grapentine, F. al-Murshedi, A. Abot, A. Weidinger, C. Kutchukian, C. Sanchez, S.J.F. Cronin, M. Novatchkova, A. Kavirayani, T. Schuetz, B. Haubner, L. Haas, A. Hagelkruys, S. Jackowski, A. Kozlov, V. Jacquemond, C. Knauf, G. Superti-Furga, E. Rullman, T. Gustafsson, J. McDermot, M. Lowe, Z. Radak, J.S. Chamberlain, M. Bakovic, S. Banka, J.M. Penninger, Nature Metabolism 5 (2023) 495–515.","ista":"Cikes D, Elsayad K, Sezgin E, Koitai E, Ferenc T, Orthofer M, Yarwood R, Heinz LX, Sedlyarov V, Darwish-Miranda N, Taylor A, Grapentine S, al-Murshedi F, Abot A, Weidinger A, Kutchukian C, Sanchez C, Cronin SJF, Novatchkova M, Kavirayani A, Schuetz T, Haubner B, Haas L, Hagelkruys A, Jackowski S, Kozlov A, Jacquemond V, Knauf C, Superti-Furga G, Rullman E, Gustafsson T, McDermot J, Lowe M, Radak Z, Chamberlain JS, Bakovic M, Banka S, Penninger JM. 2023. PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing. Nature Metabolism. 5, 495–515."},"pmid":1,"title":"PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing","department":[{"_id":"Bio"}],"quality_controlled":"1","main_file_link":[{"url":"https://doi.org/10.1101/2022.03.02.482658","open_access":"1"}],"date_created":"2023-03-23T12:58:43Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"12747","publication_identifier":{"issn":["2522-5812"]}},{"keyword":["Synchronization","Collective Movement","Active Matter","Cell Migration","Active Colloids"],"related_material":{"record":[{"id":"461","status":"public","relation":"part_of_dissertation"},{"id":"10791","status":"public","relation":"part_of_dissertation"},{"status":"public","relation":"part_of_dissertation","id":"7932"},{"status":"public","relation":"part_of_dissertation","id":"10703"},{"relation":"old_edition","status":"public","id":"12726"}]},"year":"2023","publication_status":"published","ddc":["530","570"],"alternative_title":["ISTA Thesis"],"oa":1,"month":"11","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"date_created":"2023-11-15T09:59:03Z","title":"Synchronization in collectively moving active matter","department":[{"_id":"GradSch"},{"_id":"MiSi"}],"citation":{"ama":"Riedl M. Synchronization in collectively moving active matter. 2023. doi:<a href=\"https://doi.org/10.15479/14530\">10.15479/14530</a>","apa":"Riedl, M. (2023). <i>Synchronization in collectively moving active matter</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/14530\">https://doi.org/10.15479/14530</a>","ista":"Riedl M. 2023. Synchronization in collectively moving active matter. Institute of Science and Technology Austria.","short":"M. Riedl, Synchronization in Collectively Moving Active Matter, Institute of Science and Technology Austria, 2023.","mla":"Riedl, Michael. <i>Synchronization in Collectively Moving Active Matter</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/14530\">10.15479/14530</a>.","chicago":"Riedl, Michael. “Synchronization in Collectively Moving Active Matter.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/14530\">https://doi.org/10.15479/14530</a>.","ieee":"M. Riedl, “Synchronization in collectively moving active matter,” Institute of Science and Technology Austria, 2023."},"has_accepted_license":"1","OA_place":"publisher","publication_identifier":{"issn":["2663-337X"]},"_id":"14530","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","page":"260","doi":"10.15479/14530","article_processing_charge":"No","date_published":"2023-11-16T00:00:00Z","language":[{"iso":"eng"}],"file_date_updated":"2023-11-15T09:52:54Z","corr_author":"1","abstract":[{"lang":"eng","text":"Most motions of many-body systems at any scale in nature with sufficient degrees of freedom tend to be chaotic; reaching from the orbital motion of planets, the air currents in our atmosphere, down to the water flowing through our pipelines or the movement of a population of bacteria. To the observer it is therefore intriguing when a moving collective exhibits order. Collective motion of flocks of birds, schools of fish or swarms of self-propelled particles or robots have been studied extensively over the past decades but the mechanisms involved in the transition from chaos to order remain unclear. Here, the interactions, that in most systems give rise to chaos, sustain order.  In this thesis we investigate mechanisms that preserve, destabilize or lead to the ordered state. We show that endothelial cells migrating in circular confinements transition to a collective rotating state and concomitantly synchronize the frequencies of nucleating actin waves within individual cells. Consequently, the frequency dependent cell migration speed uniformizes across the population. Complementary to the WAVE dependent nucleation of traveling actin waves, we show that in leukocytes the actin polymerization depending on WASp generates pushing forces locally at stationary patches. Next, in pipe flows, we study methods to disrupt the self--sustaining cycle of turbulence and therefore relaminarize the flow. While we find in pulsating flow conditions that turbulence emerges through a helical instability during the decelerating phase. Finally, we show quantitatively in brain slices of mice that wild-type control neurons can compensate the migratory deficits of a genetically modified neuronal sub--population in the developing cortex.  "}],"status":"public","file":[{"relation":"main_file","checksum":"52e1d0ab6c1abe59c82dfe8c9ff5f83a","date_created":"2023-11-15T09:52:54Z","success":1,"content_type":"application/pdf","file_size":36743942,"creator":"mriedl","file_id":"14536","date_updated":"2023-11-15T09:52:54Z","file_name":"Thesis_Riedl_2023_corr.pdf","access_level":"open_access"}],"oa_version":"Updated Version","type":"dissertation","degree_awarded":"PhD","supervisor":[{"orcid":"0000-0003-2057-2754","id":"3A374330-F248-11E8-B48F-1D18A9856A87","first_name":"Björn","last_name":"Hof","full_name":"Hof, Björn"}],"publisher":"Institute of Science and Technology Austria","author":[{"orcid":"0000-0003-4844-6311","first_name":"Michael","id":"3BE60946-F248-11E8-B48F-1D18A9856A87","full_name":"Riedl, Michael","last_name":"Riedl"}],"day":"16","date_updated":"2026-04-07T13:29:13Z"},{"publication_identifier":{"eissn":["1548-7105"],"issn":["1548-7091"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"14770","quality_controlled":"1","department":[{"_id":"JoDa"}],"title":"LIONESS enables 4D nanoscale reconstruction of living brain tissue","date_created":"2024-01-10T08:07:15Z","citation":{"apa":"Danzl, J. G., &#38; Velicky, P. (2023). LIONESS enables 4D nanoscale reconstruction of living brain tissue. <i>Nature Methods</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41592-023-01937-5\">https://doi.org/10.1038/s41592-023-01937-5</a>","ama":"Danzl JG, Velicky P. LIONESS enables 4D nanoscale reconstruction of living brain tissue. <i>Nature Methods</i>. 2023;20(8):1141-1142. doi:<a href=\"https://doi.org/10.1038/s41592-023-01937-5\">10.1038/s41592-023-01937-5</a>","ista":"Danzl JG, Velicky P. 2023. LIONESS enables 4D nanoscale reconstruction of living brain tissue. Nature Methods. 20(8), 1141–1142.","short":"J.G. Danzl, P. Velicky, Nature Methods 20 (2023) 1141–1142.","mla":"Danzl, Johann G., and Philipp Velicky. “LIONESS Enables 4D Nanoscale Reconstruction of Living Brain Tissue.” <i>Nature Methods</i>, vol. 20, no. 8, Springer Nature, 2023, pp. 1141–42, doi:<a href=\"https://doi.org/10.1038/s41592-023-01937-5\">10.1038/s41592-023-01937-5</a>.","chicago":"Danzl, Johann G, and Philipp Velicky. “LIONESS Enables 4D Nanoscale Reconstruction of Living Brain Tissue.” <i>Nature Methods</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41592-023-01937-5\">https://doi.org/10.1038/s41592-023-01937-5</a>.","ieee":"J. G. Danzl and P. Velicky, “LIONESS enables 4D nanoscale reconstruction of living brain tissue,” <i>Nature Methods</i>, vol. 20, no. 8. Springer Nature, pp. 1141–1142, 2023."},"scopus_import":"1","article_type":"letter_note","publication_status":"published","month":"08","related_material":{"record":[{"id":"13267","relation":"extended_version","status":"public"}]},"keyword":["Cell Biology","Molecular Biology","Biochemistry","Biotechnology"],"intvolume":"        20","year":"2023","external_id":{"isi":["001025621500002"]},"day":"01","isi":1,"date_updated":"2026-04-14T08:34:34Z","type":"journal_article","publisher":"Springer Nature","author":[{"first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973","full_name":"Danzl, Johann G","last_name":"Danzl"},{"orcid":"0000-0002-2340-7431","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87","first_name":"Philipp","full_name":"Velicky, Philipp","last_name":"Velicky"}],"publication":"Nature Methods","issue":"8","status":"public","corr_author":"1","abstract":[{"lang":"eng","text":"We developed LIONESS, a technology that leverages improvements to optical super-resolution microscopy and prior information on sample structure via machine learning to overcome the limitations (in 3D-resolution, signal-to-noise ratio and light exposure) of optical microscopy of living biological specimens. LIONESS enables dense reconstruction of living brain tissue and morphodynamics visualization at the nanoscale."}],"oa_version":"None","volume":20,"doi":"10.1038/s41592-023-01937-5","article_processing_charge":"No","page":"1141-1142","date_published":"2023-08-01T00:00:00Z","language":[{"iso":"eng"}]},{"type":"journal_article","publisher":"Elsevier","author":[{"last_name":"Westerich","full_name":"Westerich, Kim Joana","first_name":"Kim Joana"},{"last_name":"Tarbashevich","full_name":"Tarbashevich, Katsiaryna","first_name":"Katsiaryna"},{"full_name":"Schick, Jan","last_name":"Schick","first_name":"Jan"},{"full_name":"Gupta, Antra","last_name":"Gupta","first_name":"Antra"},{"first_name":"Mingzhao","full_name":"Zhu, Mingzhao","last_name":"Zhu"},{"first_name":"Kenneth","full_name":"Hull, Kenneth","last_name":"Hull"},{"first_name":"Daniel","last_name":"Romo","full_name":"Romo, Daniel"},{"last_name":"Zeuschner","full_name":"Zeuschner, Dagmar","first_name":"Dagmar"},{"first_name":"Mohammad","id":"3384113A-F248-11E8-B48F-1D18A9856A87","full_name":"Goudarzi, Mohammad","last_name":"Goudarzi"},{"full_name":"Gross-Thebing, Theresa","last_name":"Gross-Thebing","first_name":"Theresa"},{"first_name":"Erez","full_name":"Raz, Erez","last_name":"Raz"}],"day":"11","date_updated":"2024-01-16T08:56:36Z","page":"1578-1592.e5","article_processing_charge":"No","doi":"10.1016/j.devcel.2023.06.009","volume":58,"language":[{"iso":"eng"}],"date_published":"2023-09-11T00:00:00Z","abstract":[{"lang":"eng","text":"Germ granules, condensates of phase-separated RNA and protein, are organelles that are essential for germline development in different organisms. The patterning of the granules and their relevance for germ cell fate are not fully understood. Combining three-dimensional in vivo structural and functional analyses, we study the dynamic spatial organization of molecules within zebrafish germ granules. We find that the localization of RNA molecules to the periphery of the granules, where ribosomes are localized, depends on translational activity at this location. In addition, we find that the vertebrate-specific Dead end (Dnd1) protein is essential for nanos3 RNA localization at the condensates’ periphery. Accordingly, in the absence of Dnd1, or when translation is inhibited, nanos3 RNA translocates into the granule interior, away from the ribosomes, a process that is correlated with the loss of germ cell fate. These findings highlight the relevance of sub-granule compartmentalization for post-transcriptional control and its importance for preserving germ cell totipotency."}],"status":"public","issue":"17","publication":"Developmental Cell","oa_version":"Preprint","main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2023.07.09.548244"}],"date_created":"2024-01-10T09:41:21Z","title":"Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1","department":[{"_id":"Bio"}],"quality_controlled":"1","pmid":1,"citation":{"mla":"Westerich, Kim Joana, et al. “Spatial Organization and Function of RNA Molecules within Phase-Separated Condensates in Zebrafish Are Controlled by Dnd1.” <i>Developmental Cell</i>, vol. 58, no. 17, Elsevier, 2023, p. 1578–1592.e5, doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.06.009\">10.1016/j.devcel.2023.06.009</a>.","chicago":"Westerich, Kim Joana, Katsiaryna Tarbashevich, Jan Schick, Antra Gupta, Mingzhao Zhu, Kenneth Hull, Daniel Romo, et al. “Spatial Organization and Function of RNA Molecules within Phase-Separated Condensates in Zebrafish Are Controlled by Dnd1.” <i>Developmental Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.devcel.2023.06.009\">https://doi.org/10.1016/j.devcel.2023.06.009</a>.","ieee":"K. J. Westerich <i>et al.</i>, “Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1,” <i>Developmental Cell</i>, vol. 58, no. 17. Elsevier, p. 1578–1592.e5, 2023.","ama":"Westerich KJ, Tarbashevich K, Schick J, et al. Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1. <i>Developmental Cell</i>. 2023;58(17):1578-1592.e5. doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.06.009\">10.1016/j.devcel.2023.06.009</a>","apa":"Westerich, K. J., Tarbashevich, K., Schick, J., Gupta, A., Zhu, M., Hull, K., … Raz, E. (2023). Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2023.06.009\">https://doi.org/10.1016/j.devcel.2023.06.009</a>","ista":"Westerich KJ, Tarbashevich K, Schick J, Gupta A, Zhu M, Hull K, Romo D, Zeuschner D, Goudarzi M, Gross-Thebing T, Raz E. 2023. Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1. Developmental Cell. 58(17), 1578–1592.e5.","short":"K.J. Westerich, K. Tarbashevich, J. Schick, A. Gupta, M. Zhu, K. Hull, D. Romo, D. Zeuschner, M. Goudarzi, T. Gross-Thebing, E. Raz, Developmental Cell 58 (2023) 1578–1592.e5."},"publication_identifier":{"issn":["1534-5807"]},"_id":"14781","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"        58","keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"external_id":{"pmid":["37463577"]},"year":"2023","acknowledgement":"We thank Celeste Brennecka for editing and Michal Reichman-Fried for critical comments on the manuscript. We thank Ursula Jordan, Esther Messerschmidt, and Ines Sandbote for technical assistance. This work was supported by funding from the University of Münster (K.J.W., K.T., E.R., A.G., T.G.-T., J.S., and M.G.), the Max Planck Institute for Molecular Biomedicine (D.Z.), the German Research Foundation grant CRU 326 (P2) RA863/12-2 (E.R.), Baylor University (K.H. and D.R.), and the National Institutes of Health grant R35 GM 134910 (D.R.). We thank the referees for insightful comments that helped improve the manuscript.","publication_status":"published","oa":1,"article_type":"original","month":"09"},{"type":"journal_article","author":[{"first_name":"Markus","full_name":"Mund, Markus","last_name":"Mund"},{"full_name":"Tschanz, Aline","last_name":"Tschanz","first_name":"Aline"},{"first_name":"Yu-Le","full_name":"Wu, Yu-Le","last_name":"Wu"},{"last_name":"Frey","full_name":"Frey, Felix F","id":"a0270b37-8f1a-11ec-95c7-8e710c59a4f3","first_name":"Felix F","orcid":"0000-0001-8501-6017"},{"first_name":"Johanna L.","full_name":"Mehl, Johanna L.","last_name":"Mehl"},{"last_name":"Kaksonen","full_name":"Kaksonen, Marko","first_name":"Marko"},{"full_name":"Avinoam, Ori","last_name":"Avinoam","first_name":"Ori"},{"last_name":"Schwarz","full_name":"Schwarz, Ulrich S.","first_name":"Ulrich S."},{"first_name":"Jonas","last_name":"Ries","full_name":"Ries, Jonas"}],"publisher":"Rockefeller University Press","day":"03","isi":1,"date_updated":"2024-01-16T10:17:05Z","volume":222,"article_processing_charge":"No","doi":"10.1083/jcb.202206038","file_date_updated":"2024-01-16T10:15:09Z","language":[{"iso":"eng"}],"date_published":"2023-02-03T00:00:00Z","publication":"Journal of Cell Biology","file":[{"content_type":"application/pdf","file_size":5678069,"creator":"dernst","access_level":"open_access","date_updated":"2024-01-16T10:15:09Z","file_id":"14811","file_name":"2023_JCB_Mund.pdf","checksum":"505d5cac36c14b073b68c7fed1a92bd3","date_created":"2024-01-16T10:15:09Z","relation":"main_file","success":1}],"issue":"3","status":"public","abstract":[{"text":"Eukaryotic cells use clathrin-mediated endocytosis to take up a large range of extracellular cargo. During endocytosis, a clathrin coat forms on the plasma membrane, but it remains controversial when and how it is remodeled into a spherical vesicle.\r\nHere, we use 3D superresolution microscopy to determine the precise geometry of the clathrin coat at large numbers of endocytic sites. Through pseudo-temporal sorting, we determine the average trajectory of clathrin remodeling during endocytosis. We find that clathrin coats assemble first on flat membranes to 50% of the coat area before they become rapidly and continuously bent, and this mechanism is confirmed in three cell lines. We introduce the cooperative curvature model, which is based on positive feedback for curvature generation. It accurately describes the measured shapes and dynamics of the clathrin coat and could represent a general mechanism for clathrin coat remodeling on the plasma membrane.","lang":"eng"}],"oa_version":"Published Version","department":[{"_id":"AnSa"}],"quality_controlled":"1","title":"Clathrin coats partially preassemble and subsequently bend during endocytosis","date_created":"2024-01-10T10:45:55Z","has_accepted_license":"1","pmid":1,"citation":{"ista":"Mund M, Tschanz A, Wu Y-L, Frey FF, Mehl JL, Kaksonen M, Avinoam O, Schwarz US, Ries J. 2023. Clathrin coats partially preassemble and subsequently bend during endocytosis. Journal of Cell Biology. 222(3), e202206038.","short":"M. Mund, A. Tschanz, Y.-L. Wu, F.F. Frey, J.L. Mehl, M. Kaksonen, O. Avinoam, U.S. Schwarz, J. Ries, Journal of Cell Biology 222 (2023).","ama":"Mund M, Tschanz A, Wu Y-L, et al. Clathrin coats partially preassemble and subsequently bend during endocytosis. <i>Journal of Cell Biology</i>. 2023;222(3). doi:<a href=\"https://doi.org/10.1083/jcb.202206038\">10.1083/jcb.202206038</a>","apa":"Mund, M., Tschanz, A., Wu, Y.-L., Frey, F. F., Mehl, J. L., Kaksonen, M., … Ries, J. (2023). Clathrin coats partially preassemble and subsequently bend during endocytosis. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202206038\">https://doi.org/10.1083/jcb.202206038</a>","chicago":"Mund, Markus, Aline Tschanz, Yu-Le Wu, Felix F Frey, Johanna L. Mehl, Marko Kaksonen, Ori Avinoam, Ulrich S. Schwarz, and Jonas Ries. “Clathrin Coats Partially Preassemble and Subsequently Bend during Endocytosis.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2023. <a href=\"https://doi.org/10.1083/jcb.202206038\">https://doi.org/10.1083/jcb.202206038</a>.","ieee":"M. Mund <i>et al.</i>, “Clathrin coats partially preassemble and subsequently bend during endocytosis,” <i>Journal of Cell Biology</i>, vol. 222, no. 3. Rockefeller University Press, 2023.","mla":"Mund, Markus, et al. “Clathrin Coats Partially Preassemble and Subsequently Bend during Endocytosis.” <i>Journal of Cell Biology</i>, vol. 222, no. 3, e202206038, Rockefeller University Press, 2023, doi:<a href=\"https://doi.org/10.1083/jcb.202206038\">10.1083/jcb.202206038</a>."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_number":"e202206038","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"14788","keyword":["Cell Biology"],"intvolume":"       222","year":"2023","external_id":{"pmid":["36734980"],"isi":["000978065000001"]},"article_type":"original","oa":1,"ddc":["570"],"acknowledgement":"We thank the entire Ries and Kaksonen labs for fruitful discussions and support. This work was supported by the European Research Council (ERC CoG-724489 to J. Ries), the National Institutes of Health Common Fund 4D Nucleome Program (Grant U01 to J. Ries), the Human Frontier Science Program (RGY0065/2017 to J. Ries), the EMBL Interdisciplinary Postdoc Programme (EIPOD) under Marie Curie Actions COFUND (Grant 229597 to O. Avinoam), the European Molecular Biology Laboratory (M. Mund, A. Tschanz, Y.-L. Wu and J. Ries), and the Swiss National Science Foundation (grant 310030B_182825 and NCCR Chemical Biology to M. Kaksonen). O. Avinoam is an incumbent of the Miriam Berman Presidential Development Chair.","publication_status":"published","month":"02"},{"publisher":"The Company of Biologists","author":[{"id":"3436488C-F248-11E8-B48F-1D18A9856A87","first_name":"Cornelia","orcid":"0000-0001-5130-2226","last_name":"Schwayer","full_name":"Schwayer, Cornelia"},{"last_name":"Brückner","full_name":"Brückner, David","orcid":"0000-0001-7205-2975","id":"e1e86031-6537-11eb-953a-f7ab92be508d","first_name":"David"}],"type":"journal_article","date_updated":"2025-09-09T14:22:02Z","day":"27","isi":1,"project":[{"_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development"}],"date_published":"2023-12-27T00:00:00Z","language":[{"iso":"eng"}],"volume":136,"doi":"10.1242/jcs.261515","article_processing_charge":"No","oa_version":"None","publication":"Journal of Cell Science","issue":"24","status":"public","abstract":[{"text":"Understanding complex living systems, which are fundamentally constrained by physical phenomena, requires combining experimental data with theoretical physical and mathematical models. To develop such models, collaborations between experimental cell biologists and theoreticians are increasingly important but these two groups often face challenges achieving mutual understanding. To help navigate these challenges, this Perspective discusses different modelling approaches, including bottom-up hypothesis-driven and top-down data-driven models, and highlights their strengths and applications. Using cell mechanics as an example, we explore the integration of specific physical models with experimental data from the molecular, cellular and tissue level up to multiscale input. We also emphasize the importance of constraining model complexity and outline strategies for crosstalk between experimental design and model development. Furthermore, we highlight how physical models can provide conceptual insights and produce unifying and generalizable frameworks for biological phenomena. Overall, this Perspective aims to promote fruitful collaborations that advance our understanding of complex biological systems.","lang":"eng"}],"corr_author":"1","pmid":1,"citation":{"ista":"Schwayer C, Brückner D. 2023. Connecting theory and experiment in cell and tissue mechanics. Journal of Cell Science. 136(24), jcs. 261515.","short":"C. Schwayer, D. Brückner, Journal of Cell Science 136 (2023).","apa":"Schwayer, C., &#38; Brückner, D. (2023). Connecting theory and experiment in cell and tissue mechanics. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.261515\">https://doi.org/10.1242/jcs.261515</a>","ama":"Schwayer C, Brückner D. Connecting theory and experiment in cell and tissue mechanics. <i>Journal of Cell Science</i>. 2023;136(24). doi:<a href=\"https://doi.org/10.1242/jcs.261515\">10.1242/jcs.261515</a>","chicago":"Schwayer, Cornelia, and David Brückner. “Connecting Theory and Experiment in Cell and Tissue Mechanics.” <i>Journal of Cell Science</i>. The Company of Biologists, 2023. <a href=\"https://doi.org/10.1242/jcs.261515\">https://doi.org/10.1242/jcs.261515</a>.","ieee":"C. Schwayer and D. Brückner, “Connecting theory and experiment in cell and tissue mechanics,” <i>Journal of Cell Science</i>, vol. 136, no. 24. The Company of Biologists, 2023.","mla":"Schwayer, Cornelia, and David Brückner. “Connecting Theory and Experiment in Cell and Tissue Mechanics.” <i>Journal of Cell Science</i>, vol. 136, no. 24, jcs. 261515, The Company of Biologists, 2023, doi:<a href=\"https://doi.org/10.1242/jcs.261515\">10.1242/jcs.261515</a>."},"quality_controlled":"1","department":[{"_id":"EdHa"},{"_id":"CaHe"}],"title":"Connecting theory and experiment in cell and tissue mechanics","date_created":"2024-01-17T12:46:55Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","_id":"14827","article_number":"jcs.261515","publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"year":"2023","external_id":{"isi":["001165394900011"],"pmid":["38149871"]},"keyword":["Cell Biology"],"intvolume":"       136","month":"12","article_type":"original","scopus_import":"1","acknowledgement":"We thank Prisca Liberali and Edouard Hannezo for many inspiring discussions; Mehmet Can Uçar, Nicoletta I Petridou and Qiutan Yang for a critical reading of the manuscript, and Claudia Flandoli for the artwork in Figs 2 and 3. We would also like to thank The Company of Biologists for the opportunity to attend the 2023 workshop on Collective Cell Migration, and all workshop participants for discussions.\r\nC.S. was supported by a European Molecular Biology Organization (EMBO) Postdoctoral Fellowship (ALTF 660-2020) and Human Frontier Science Program (HFSP) Postdoctoral fellowship (LT000746/2021-L). D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022).","publication_status":"published"},{"page":"746-758.e5","doi":"10.1016/j.molcel.2023.01.024","article_processing_charge":"Yes (in subscription journal)","volume":83,"language":[{"iso":"eng"}],"date_published":"2023-03-02T00:00:00Z","abstract":[{"text":"Type I CRISPR-Cas systems employ multi-subunit Cascade effector complexes to target foreign nucleic acids for destruction. Here, we present structures of D. vulgaris type I-C Cascade at various stages of double-stranded (ds)DNA target capture, revealing mechanisms that underpin PAM recognition and Cascade allosteric activation. We uncover an interesting mechanism of non-target strand (NTS) DNA stabilization via stacking interactions with the “belly” subunits, securing the NTS in place. This “molecular seatbelt” mechanism facilitates efficient R-loop formation and prevents dsDNA reannealing. Additionally, we provide structural insights into how two anti-CRISPR (Acr) proteins utilize distinct strategies to achieve a shared mechanism of type I-C Cascade inhibition by blocking PAM scanning. These observations form a structural basis for directional R-loop formation and reveal how different Acr proteins have converged upon common molecular mechanisms to efficiently shut down CRISPR immunity.","lang":"eng"}],"status":"public","issue":"5","publication":"Molecular Cell","oa_version":"Published Version","type":"journal_article","publisher":"Elsevier","author":[{"last_name":"O’Brien","full_name":"O’Brien, Roisin E.","first_name":"Roisin E."},{"full_name":"Bravo, Jack Peter Kelly","last_name":"Bravo","orcid":"0000-0003-0456-0753","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","first_name":"Jack Peter Kelly"},{"full_name":"Ramos, Delisa","last_name":"Ramos","first_name":"Delisa"},{"last_name":"Hibshman","full_name":"Hibshman, Grace N.","first_name":"Grace N."},{"first_name":"Jacquelyn T.","last_name":"Wright","full_name":"Wright, Jacquelyn T."},{"first_name":"David W.","last_name":"Taylor","full_name":"Taylor, David W."}],"day":"02","date_updated":"2024-06-04T06:33:54Z","intvolume":"        83","keyword":["Cell Biology","Molecular Biology"],"external_id":{"pmid":["36805026"]},"year":"2023","publication_status":"published","scopus_import":"1","oa":1,"article_type":"original","month":"03","main_file_link":[{"url":"https://doi.org/10.1016/j.molcel.2023.01.024","open_access":"1"}],"date_created":"2024-03-20T10:40:56Z","extern":"1","title":"Structural snapshots of R-loop formation by a type I-C CRISPR Cascade","quality_controlled":"1","pmid":1,"citation":{"mla":"O’Brien, Roisin E., et al. “Structural Snapshots of R-Loop Formation by a Type I-C CRISPR Cascade.” <i>Molecular Cell</i>, vol. 83, no. 5, Elsevier, 2023, p. 746–758.e5, doi:<a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">10.1016/j.molcel.2023.01.024</a>.","ieee":"R. E. O’Brien, J. P. K. Bravo, D. Ramos, G. N. Hibshman, J. T. Wright, and D. W. Taylor, “Structural snapshots of R-loop formation by a type I-C CRISPR Cascade,” <i>Molecular Cell</i>, vol. 83, no. 5. Elsevier, p. 746–758.e5, 2023.","chicago":"O’Brien, Roisin E., Jack Peter Kelly Bravo, Delisa Ramos, Grace N. Hibshman, Jacquelyn T. Wright, and David W. Taylor. “Structural Snapshots of R-Loop Formation by a Type I-C CRISPR Cascade.” <i>Molecular Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">https://doi.org/10.1016/j.molcel.2023.01.024</a>.","apa":"O’Brien, R. E., Bravo, J. P. K., Ramos, D., Hibshman, G. N., Wright, J. T., &#38; Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. <i>Molecular Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">https://doi.org/10.1016/j.molcel.2023.01.024</a>","ama":"O’Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. <i>Molecular Cell</i>. 2023;83(5):746-758.e5. doi:<a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">10.1016/j.molcel.2023.01.024</a>","short":"R.E. O’Brien, J.P.K. Bravo, D. Ramos, G.N. Hibshman, J.T. Wright, D.W. Taylor, Molecular Cell 83 (2023) 746–758.e5.","ista":"O’Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. 2023. Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular Cell. 83(5), 746–758.e5."},"publication_identifier":{"issn":["1097-2765"]},"_id":"15129","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"file":[{"relation":"main_file","checksum":"069d87f025e0799bf9e3c375664264f2","date_created":"2023-02-07T13:07:38Z","embargo":"2024-02-07","content_type":"application/pdf","date_updated":"2024-02-08T23:30:04Z","file_name":"PhDThesis_BettinaZens_2023_final.pdf","file_id":"12527","access_level":"open_access","creator":"bzens","file_size":23082464},{"access_level":"closed","file_id":"12528","file_name":"PhDThesis_BettinaZens_2023_final.docx","date_updated":"2024-02-08T23:30:04Z","creator":"bzens","file_size":106169509,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","checksum":"8c66ed203495d6e078ed1002a866520c","embargo_to":"open_access","date_created":"2023-02-07T13:09:05Z","relation":"source_file"}],"corr_author":"1","abstract":[{"lang":"eng","text":"The extracellular matrix (ECM) is a hydrated and complex three-dimensional network consisting of proteins, polysaccharides, and water. It provides structural scaffolding for the cells embedded within it and is essential in regulating numerous physiological processes, including cell migration and proliferation, wound healing, and stem cell fate. \r\nDespite extensive study, detailed structural knowledge of ECM components in physiologically relevant conditions is still rudimentary. This is due to methodological limitations in specimen preparation protocols which are incompatible with keeping large samples, such as the ECM, in their native state for subsequent imaging. Conventional electron microscopy (EM) techniques rely on fixation, dehydration, contrasting, and sectioning. This results in the alteration of a highly hydrated environment and the potential introduction of artifacts. Other structural biology techniques, such as nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography, allow high-resolution analysis of protein structures but only work on homogenous and purified samples, hence lacking contextual information. Currently, no approach exists for the ultrastructural and structural study of extracellular components under native conditions in a physiological, 3D environment. \r\nIn this thesis, I have developed a workflow that allows for the ultrastructural analysis of the ECM in near-native conditions at molecular resolution. The developments I introduced include implementing a novel specimen preparation workflow for cell-derived matrices (CDMs) to render them compatible with ion-beam milling and subsequent high-resolution cryo-electron tomography (ET). \r\nTo this end, I have established protocols to generate CDMs grown over several weeks on EM grids that are compatible with downstream cryo-EM sample preparation and imaging techniques. Characterization of these ECMs confirmed that they contain essential ECM components such as collagen I, collagen VI, and fibronectin I in high abundance and hence represent a bona fide biologically-relevant sample. I successfully optimized vitrification of these specimens by testing various vitrification techniques and cryoprotectants. \r\nIn order to obtain high-resolution molecular insights into the ultrastructure and organization of CDMs, I established cryo-focused ion beam scanning electron microscopy (FIBSEM) on these challenging and complex specimens. I explored different approaches for the creation of thin cryo-lamellae by FIB milling and succeeded in optimizing the cryo-lift-out technique, resulting in high-quality lamellae of approximately 200 nm thickness. \r\nHigh-resolution Cryo-ET of these lamellae revealed for the first time the architecture of native CDM in the context of matrix-secreting cells. This allowed for the in situ visualization of fibrillar matrix proteins such as collagen, laying the foundation for future structural and ultrastructural characterization of these proteins in their near-native environment. \r\nIn summary, in this thesis, I present a novel workflow that combines state-of-the-art cryo-EM specimen preparation and imaging technologies to permit characterization of the ECM, an important tissue component in higher organisms. This innovative and highly versatile workflow will enable addressing far-reaching questions on ECM architecture, composition, and reciprocal ECM-cell interactions."}],"status":"public","oa_version":"Published Version","page":"187","article_processing_charge":"No","doi":"10.15479/at:ista:12491","language":[{"iso":"eng"}],"date_published":"2023-02-02T00:00:00Z","file_date_updated":"2024-02-08T23:30:04Z","day":"02","project":[{"name":"Integrated visual proteomics of reciprocal cell-extracellular matrix interactions","_id":"eba3b5f6-77a9-11ec-83b8-cf0905748aa3"},{"_id":"059B463C-7A3F-11EA-A408-12923DDC885E","name":"NÖ-Fonds Preis für die Jungforscherin des Jahres am IST Austria"}],"date_updated":"2026-04-07T13:49:23Z","type":"dissertation","degree_awarded":"PhD","author":[{"last_name":"Zens","full_name":"Zens, Bettina","orcid":"0000-0002-9561-1239","first_name":"Bettina","id":"45FD126C-F248-11E8-B48F-1D18A9856A87"}],"publisher":"Institute of Science and Technology Austria","supervisor":[{"last_name":"Schur","full_name":"Schur, Florian KM","first_name":"Florian KM","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4790-8078"}],"alternative_title":["ISTA Thesis"],"oa":1,"publication_status":"published","ddc":["570"],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"Bio"}],"month":"02","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"8586"}]},"keyword":["cryo-EM","cryo-ET","FIB milling","method development","FIBSEM","extracellular matrix","ECM","cell-derived matrices","CDMs","cell culture","high pressure freezing","HPF","structural biology","tomography","collagen"],"year":"2023","OA_place":"publisher","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-027-5"]},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","_id":"12491","title":"Ultrastructural characterization of natively preserved extracellular matrix by cryo-electron tomography","department":[{"_id":"GradSch"},{"_id":"FlSc"}],"date_created":"2023-02-02T14:50:20Z","citation":{"ama":"Zens B. Ultrastructural characterization of natively preserved extracellular matrix by cryo-electron tomography. 2023. doi:<a href=\"https://doi.org/10.15479/at:ista:12491\">10.15479/at:ista:12491</a>","apa":"Zens, B. (2023). <i>Ultrastructural characterization of natively preserved extracellular matrix by cryo-electron tomography</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:12491\">https://doi.org/10.15479/at:ista:12491</a>","short":"B. Zens, Ultrastructural Characterization of Natively Preserved Extracellular Matrix by Cryo-Electron Tomography, Institute of Science and Technology Austria, 2023.","ista":"Zens B. 2023. Ultrastructural characterization of natively preserved extracellular matrix by cryo-electron tomography. Institute of Science and Technology Austria.","mla":"Zens, Bettina. <i>Ultrastructural Characterization of Natively Preserved Extracellular Matrix by Cryo-Electron Tomography</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/at:ista:12491\">10.15479/at:ista:12491</a>.","ieee":"B. Zens, “Ultrastructural characterization of natively preserved extracellular matrix by cryo-electron tomography,” Institute of Science and Technology Austria, 2023.","chicago":"Zens, Bettina. “Ultrastructural Characterization of Natively Preserved Extracellular Matrix by Cryo-Electron Tomography.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/at:ista:12491\">https://doi.org/10.15479/at:ista:12491</a>."},"has_accepted_license":"1"},{"degree_awarded":"PhD","type":"dissertation","author":[{"last_name":"Radler","full_name":"Radler, Philipp","orcid":"0000-0001-9198-2182 ","first_name":"Philipp","id":"40136C2A-F248-11E8-B48F-1D18A9856A87"}],"publisher":"Institute of Science and Technology Austria","supervisor":[{"last_name":"Loose","full_name":"Loose, Martin","orcid":"0000-0001-7309-9724","id":"462D4284-F248-11E8-B48F-1D18A9856A87","first_name":"Martin"}],"day":"25","project":[{"call_identifier":"H2020","_id":"2595697A-B435-11E9-9278-68D0E5697425","name":"Self-Organization of the Bacterial Cell","grant_number":"679239"},{"_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d","grant_number":"P34607","name":"In vitro reconstitution of bacterial cell division"},{"_id":"2596EAB6-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 2015-1163","name":"Synthesis of bacterial cell wall"},{"_id":"259B655A-B435-11E9-9278-68D0E5697425","name":"Reconstitution of bacterial cell wall synthesis","grant_number":"LT000824/2016"}],"date_updated":"2026-04-07T14:06:05Z","article_processing_charge":"No","doi":"10.15479/at:ista:14280","page":"156","file_date_updated":"2024-10-05T22:30:03Z","date_published":"2023-09-25T00:00:00Z","language":[{"iso":"eng"}],"file":[{"relation":"source_file","checksum":"87eef11fbc5c7df0826f12a3a629b444","embargo_to":"open_access","date_created":"2023-10-04T10:11:53Z","file_size":114932847,"creator":"pradler","file_id":"14390","date_updated":"2024-10-05T22:30:03Z","file_name":"PhD Thesis_Philipp Radler_20231004.docx","access_level":"closed","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document"},{"creator":"pradler","file_size":37838778,"file_name":"PhD Thesis_Philipp Radler_20231004.pdf","date_updated":"2024-10-05T22:30:03Z","file_id":"14391","access_level":"open_access","content_type":"application/pdf","embargo":"2024-10-04","relation":"main_file","checksum":"3253e099b7126469d941fd9419d68b4f","date_created":"2023-10-04T10:11:21Z"}],"status":"public","corr_author":"1","abstract":[{"text":"Cell division in Escherichia coli is performed by the divisome, a multi-protein complex composed of more than 30 proteins. The divisome spans from the cytoplasm through the inner membrane to the cell wall and the outer membrane. Divisome assembly is initiated by a cytoskeletal structure, the so-called Z-ring, which localizes at the center of the E. coli cell and determines the position of the future cell septum. The Z-ring is composed of the highly conserved bacterial tubulin homologue FtsZ, which forms treadmilling filaments. These filaments are recruited to the inner membrane by FtsA, a highly conserved bacterial actin homologue. FtsA interacts with other proteins in the periplasm and thus connects the cytoplasmic and periplasmic components of the divisome. \r\nA previous model postulated that FtsA regulates maturation of the divisome by switching from an oligomeric, inactive state to a monomeric and active state. This model was based mostly on in vivo studies, as a biochemical characterization of FtsA has been hampered by difficulties in purifying the protein. Here, we studied FtsA using an in vitro reconstitution approach and aimed to answer two questions: (i) How are dynamics from cytoplasmic, treadmilling FtsZ filaments coupled to proteins acting in the periplasmic space and (ii) How does FtsA regulate the maturation of the divisome?\r\nWe found that the cytoplasmic peptides of the transmembrane proteins FtsN and FtsQ interact directly with FtsA and can follow the spatiotemporal signal of FtsA/Z filaments. When we investigated the underlying mechanism by imaging single molecules of FtsNcyto, we found the peptide to interact transiently with FtsA. An in depth analysis of the single molecule trajectories helped to postulate a model where PG synthases follow the dynamics of FtsZ by a diffusion and capture mechanism. \r\nFollowing up on these findings we were interested in how the self-interaction of FtsA changes when it encounters FtsNcyto and if we can confirm the proposed oligomer-monomer switch. For this, we compared the behavior of the previously identified, hyperactive mutant FtsA R286W with wildtype FtsA. The mutant outperforms WT in mirroring and transmitting the spatiotemporal signal of treadmilling FtsZ filaments. Surprisingly however, we found that this was not due to a difference in the self-interaction strength of the two variants, but a difference in their membrane residence time. Furthermore, in contrast to our expectations, upon binding of FtsNcyto the measured self-interaction of FtsA actually increased. \r\nWe propose that FtsNcyto induces a rearrangement of the oligomeric architecture of FtsA. In further consequence this change leads to more persistent FtsZ filaments which results in a defined signalling zone, allowing formation of the mature divisome. The observed difference between FtsA WT and R286W is due to the vastly different membrane turnover of the proteins. R286W cycles 5-10x faster compared to WT which allows to sample FtsZ filaments at faster frequencies. These findings can explain the observed differences in toxicity for overexpression of FtsA WT and R286W and help to understand how FtsA regulates divisome maturation.","lang":"eng"}],"oa_version":"Published Version","department":[{"_id":"GradSch"},{"_id":"MaLo"}],"title":"Spatiotemporal signaling during assembly of the bacterial divisome","date_created":"2023-09-06T10:58:25Z","has_accepted_license":"1","citation":{"short":"P. Radler, Spatiotemporal Signaling during Assembly of the Bacterial Divisome, Institute of Science and Technology Austria, 2023.","ista":"Radler P. 2023. Spatiotemporal signaling during assembly of the bacterial divisome. Institute of Science and Technology Austria.","apa":"Radler, P. (2023). <i>Spatiotemporal signaling during assembly of the bacterial divisome</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:14280\">https://doi.org/10.15479/at:ista:14280</a>","ama":"Radler P. Spatiotemporal signaling during assembly of the bacterial divisome. 2023. doi:<a href=\"https://doi.org/10.15479/at:ista:14280\">10.15479/at:ista:14280</a>","ieee":"P. Radler, “Spatiotemporal signaling during assembly of the bacterial divisome,” Institute of Science and Technology Austria, 2023.","chicago":"Radler, Philipp. “Spatiotemporal Signaling during Assembly of the Bacterial Divisome.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/at:ista:14280\">https://doi.org/10.15479/at:ista:14280</a>.","mla":"Radler, Philipp. <i>Spatiotemporal Signaling during Assembly of the Bacterial Divisome</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/at:ista:14280\">10.15479/at:ista:14280</a>."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"ec_funded":1,"OA_place":"publisher","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-033-6"]},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","_id":"14280","keyword":["Cell Division","Reconstitution","FtsZ","FtsA","Divisome","E.coli"],"related_material":{"record":[{"id":"10934","relation":"research_data","status":"public"},{"relation":"part_of_dissertation","status":"public","id":"11373"},{"id":"7387","relation":"part_of_dissertation","status":"public"}]},"year":"2023","oa":1,"alternative_title":["ISTA Thesis"],"ddc":["572"],"publication_status":"published","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"month":"09"},{"article_type":"review","scopus_import":"1","publication_status":"published","month":"03","keyword":["Cell Biology"],"intvolume":"        32","external_id":{"pmid":["34782239"]},"year":"2022","publication_identifier":{"issn":["0962-8924"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"11051","title":"Nuclear pore complex maintenance and implications for age-related diseases","quality_controlled":"1","date_created":"2022-04-07T07:43:01Z","extern":"1","pmid":1,"citation":{"ista":"Liu J, Hetzer M. 2022. Nuclear pore complex maintenance and implications for age-related diseases. Trends in Cell Biology. 32(3), P216-227.","short":"J. Liu, M. Hetzer, Trends in Cell Biology 32 (2022) P216-227.","apa":"Liu, J., &#38; Hetzer, M. (2022). Nuclear pore complex maintenance and implications for age-related diseases. <i>Trends in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tcb.2021.10.001\">https://doi.org/10.1016/j.tcb.2021.10.001</a>","ama":"Liu J, Hetzer M. Nuclear pore complex maintenance and implications for age-related diseases. <i>Trends in Cell Biology</i>. 2022;32(3):P216-227. doi:<a href=\"https://doi.org/10.1016/j.tcb.2021.10.001\">10.1016/j.tcb.2021.10.001</a>","chicago":"Liu, Jinqiang, and Martin Hetzer. “Nuclear Pore Complex Maintenance and Implications for Age-Related Diseases.” <i>Trends in Cell Biology</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.tcb.2021.10.001\">https://doi.org/10.1016/j.tcb.2021.10.001</a>.","ieee":"J. Liu and M. Hetzer, “Nuclear pore complex maintenance and implications for age-related diseases,” <i>Trends in Cell Biology</i>, vol. 32, no. 3. Elsevier, pp. P216-227, 2022.","mla":"Liu, Jinqiang, and Martin Hetzer. “Nuclear Pore Complex Maintenance and Implications for Age-Related Diseases.” <i>Trends in Cell Biology</i>, vol. 32, no. 3, Elsevier, 2022, pp. P216-227, doi:<a href=\"https://doi.org/10.1016/j.tcb.2021.10.001\">10.1016/j.tcb.2021.10.001</a>."},"issue":"3","publication":"Trends in Cell Biology","abstract":[{"lang":"eng","text":"Nuclear pore complexes (NPCs) bridge the nucleus and the cytoplasm and are indispensable for crucial cellular activities, such as bidirectional molecular trafficking and gene transcription regulation. The discovery of long-lived proteins (LLPs) in NPCs from postmitotic cells raises the exciting possibility that the maintenance of NPC integrity might play an inherent role in lifelong cell function. Age-dependent deterioration of NPCs and loss of nuclear integrity have been linked to age-related decline in postmitotic cell function and degenerative diseases. In this review, we discuss our current understanding of NPC maintenance in proliferating and postmitotic cells, and how malfunction of nucleoporins (Nups) might contribute to the pathogenesis of various neurodegenerative and cardiovascular diseases."}],"status":"public","oa_version":"None","volume":32,"page":"P216-227","article_processing_charge":"No","doi":"10.1016/j.tcb.2021.10.001","date_published":"2022-03-01T00:00:00Z","language":[{"iso":"eng"}],"day":"01","date_updated":"2024-10-14T11:14:57Z","type":"journal_article","author":[{"last_name":"Liu","full_name":"Liu, Jinqiang","first_name":"Jinqiang"},{"last_name":"HETZER","full_name":"HETZER, Martin W","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","first_name":"Martin W","orcid":"0000-0002-2111-992X"}],"publisher":"Elsevier"},{"publisher":"Elsevier","author":[{"first_name":"Huixin","last_name":"Xiao","full_name":"Xiao, Huixin"},{"last_name":"Hu","full_name":"Hu, Yumei","first_name":"Yumei"},{"first_name":"Yaping","last_name":"Wang","full_name":"Wang, Yaping"},{"first_name":"Jinkui","last_name":"Cheng","full_name":"Cheng, Jinkui"},{"full_name":"Wang, Jinyi","last_name":"Wang","first_name":"Jinyi"},{"full_name":"Chen, Guojingwei","last_name":"Chen","first_name":"Guojingwei"},{"first_name":"Qian","last_name":"Li","full_name":"Li, Qian"},{"full_name":"Wang, Shuwei","last_name":"Wang","first_name":"Shuwei"},{"first_name":"Yalu","full_name":"Wang, Yalu","last_name":"Wang"},{"full_name":"Wang, Shao-Shuai","last_name":"Wang","first_name":"Shao-Shuai"},{"first_name":"Yi","full_name":"Wang, Yi","last_name":"Wang"},{"last_name":"Xuan","full_name":"Xuan, Wei","first_name":"Wei"},{"full_name":"Li, Zhen","last_name":"Li","first_name":"Zhen"},{"full_name":"Guo, Yan","last_name":"Guo","first_name":"Yan"},{"last_name":"Gong","full_name":"Gong, Zhizhong","first_name":"Zhizhong"},{"last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"Jing","full_name":"Zhang, Jing","last_name":"Zhang"}],"type":"journal_article","date_updated":"2026-06-18T17:23:10Z","day":"05","isi":1,"date_published":"2022-12-05T00:00:00Z","language":[{"iso":"eng"}],"OA_type":"free access","volume":57,"doi":"10.1016/j.devcel.2022.11.006","article_processing_charge":"No","page":"2638-2651.e6","oa_version":"Published Version","publication":"Developmental Cell","issue":"23","status":"public","abstract":[{"lang":"eng","text":"Plant root architecture flexibly adapts to changing nitrate (NO3−) availability in the soil; however, the underlying molecular mechanism of this adaptive development remains under-studied. To explore the regulation of NO3−-mediated root growth, we screened for low-nitrate-resistant mutant (lonr) and identified mutants that were defective in the NAC transcription factor NAC075 (lonr1) as being less sensitive to low NO3− in terms of primary root growth. We show that NAC075 is a mobile transcription factor relocating from the root stele tissues to the endodermis based on NO3− availability. Under low-NO3− availability, the kinase CBL-interacting protein kinase 1 (CIPK1) is activated, and it phosphorylates NAC075, restricting its movement from the stele, which leads to the transcriptional regulation of downstream target WRKY53, consequently leading to adapted root architecture. Our work thus identifies an adaptive mechanism involving translocation of transcription factor based on nutrient availability and leading to cell-specific reprogramming of plant root growth."}],"pmid":1,"citation":{"ama":"Xiao H, Hu Y, Wang Y, et al. Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth. <i>Developmental Cell</i>. 2022;57(23):2638-2651.e6. doi:<a href=\"https://doi.org/10.1016/j.devcel.2022.11.006\">10.1016/j.devcel.2022.11.006</a>","apa":"Xiao, H., Hu, Y., Wang, Y., Cheng, J., Wang, J., Chen, G., … Zhang, J. (2022). Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2022.11.006\">https://doi.org/10.1016/j.devcel.2022.11.006</a>","ista":"Xiao H, Hu Y, Wang Y, Cheng J, Wang J, Chen G, Li Q, Wang S, Wang Y, Wang S-S, Wang Y, Xuan W, Li Z, Guo Y, Gong Z, Friml J, Zhang J. 2022. Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth. Developmental Cell. 57(23), 2638–2651.e6.","short":"H. Xiao, Y. Hu, Y. Wang, J. Cheng, J. Wang, G. Chen, Q. Li, S. Wang, Y. Wang, S.-S. Wang, Y. Wang, W. Xuan, Z. Li, Y. Guo, Z. Gong, J. Friml, J. Zhang, Developmental Cell 57 (2022) 2638–2651.e6.","mla":"Xiao, Huixin, et al. “Nitrate Availability Controls Translocation of the Transcription Factor NAC075 for Cell-Type-Specific Reprogramming of Root Growth.” <i>Developmental Cell</i>, vol. 57, no. 23, Elsevier, 2022, p. 2638–2651.e6, doi:<a href=\"https://doi.org/10.1016/j.devcel.2022.11.006\">10.1016/j.devcel.2022.11.006</a>.","chicago":"Xiao, Huixin, Yumei Hu, Yaping Wang, Jinkui Cheng, Jinyi Wang, Guojingwei Chen, Qian Li, et al. “Nitrate Availability Controls Translocation of the Transcription Factor NAC075 for Cell-Type-Specific Reprogramming of Root Growth.” <i>Developmental Cell</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.devcel.2022.11.006\">https://doi.org/10.1016/j.devcel.2022.11.006</a>.","ieee":"H. Xiao <i>et al.</i>, “Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth,” <i>Developmental Cell</i>, vol. 57, no. 23. Elsevier, p. 2638–2651.e6, 2022."},"quality_controlled":"1","department":[{"_id":"JiFr"}],"title":"Nitrate availability controls translocation of the transcription factor NAC075 for cell-type-specific reprogramming of root growth","date_created":"2023-01-12T11:57:00Z","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2022.11.006","open_access":"1"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"12120","OA_place":"publisher","publication_identifier":{"issn":["1534-5807"]},"year":"2022","external_id":{"isi":["000919603800005"],"pmid":["36473460"]},"keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"intvolume":"        57","month":"12","scopus_import":"1","article_type":"original","oa":1,"acknowledgement":"The authors are grateful to Jörg Kudla, Ying Miao, Yu Zheng, Gang Li, and Jun Zheng for providing published materials and to Wenkun Zhou and Caifu Jiang for helpful discussions. This work was supported by grants from the National Key Research and Development Program of China (2021YFF1000500), the National Natural Science Foundation of China (32170265 and 32022007), the Beijing Municipal Natural Science Foundation (5192011), and the Chinese Universities Scientific Fund (2022TC153).","ddc":["580"],"publication_status":"published"},{"acknowledgement":"We thank Suayip Ustün, Karin Schumacher, Erika Isono, Gerd Juergens, Takashi Ueda, Daniel Hofius, and Liwen Jiang for sharing published materials.\r\nWe acknowledge funding from Austrian Academy of Sciences, Austrian Science Fund (FWF, P 32355, P 34944), Austrian Science Fund (FWF-SFB F79), Vienna Science and Technology\r\nFund (WWTF, LS17-047) to Y. Dagdas; Austrian Academy of Sciences DOC Fellowship to J. Zhao, Marie Curie VIP2 Fellowship to J.C. De La Concepcion and M. Clavel; Hong Kong Research Grant Council (GRF14121019, 14113921, AoE/M-05/12, C4002-17G) to B.-H. Kang. We thank Vienna Biocenter Core Facilities (VBCF) Protein Chemistry, Biooptics, Plant Sciences, Molecular Biology, and Protein Technologies. We thank J. Matthew Watson\r\nand members of the Dagdas lab for the critical reading and editing of the manuscript.","publication_status":"published","ddc":["580"],"oa":1,"article_type":"original","scopus_import":"1","month":"12","intvolume":"       221","keyword":["Cell Biology"],"external_id":{"isi":["000932958800001"],"pmid":["36260289"]},"year":"2022","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"article_number":"e202203139","_id":"12121","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_created":"2023-01-12T11:57:10Z","title":"Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole","quality_controlled":"1","department":[{"_id":"JiFr"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"pmid":1,"citation":{"mla":"Zhao, Jierui, et al. “Plant Autophagosomes Mature into Amphisomes Prior to Their Delivery to the Central Vacuole.” <i>Journal of Cell Biology</i>, vol. 221, no. 12, e202203139, Rockefeller University Press, 2022, doi:<a href=\"https://doi.org/10.1083/jcb.202203139\">10.1083/jcb.202203139</a>.","chicago":"Zhao, Jierui, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti, Juan Carlos De La Concepcion, Yixuan Chen, et al. “Plant Autophagosomes Mature into Amphisomes Prior to Their Delivery to the Central Vacuole.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2022. <a href=\"https://doi.org/10.1083/jcb.202203139\">https://doi.org/10.1083/jcb.202203139</a>.","ieee":"J. Zhao <i>et al.</i>, “Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole,” <i>Journal of Cell Biology</i>, vol. 221, no. 12. Rockefeller University Press, 2022.","apa":"Zhao, J., Bui, M. T., Ma, J., Künzl, F., Picchianti, L., De La Concepcion, J. C., … Dagdas, Y. (2022). Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202203139\">https://doi.org/10.1083/jcb.202203139</a>","ama":"Zhao J, Bui MT, Ma J, et al. Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole. <i>Journal of Cell Biology</i>. 2022;221(12). doi:<a href=\"https://doi.org/10.1083/jcb.202203139\">10.1083/jcb.202203139</a>","ista":"Zhao J, Bui MT, Ma J, Künzl F, Picchianti L, De La Concepcion JC, Chen Y, Petsangouraki S, Mohseni A, García-Leon M, Gomez MS, Giannini C, Gwennogan D, Kobylinska R, Clavel M, Schellmann S, Jaillais Y, Friml J, Kang B-H, Dagdas Y. 2022. Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole. Journal of Cell Biology. 221(12), e202203139.","short":"J. Zhao, M.T. Bui, J. Ma, F. Künzl, L. Picchianti, J.C. De La Concepcion, Y. Chen, S. Petsangouraki, A. Mohseni, M. García-Leon, M.S. Gomez, C. Giannini, D. Gwennogan, R. Kobylinska, M. Clavel, S. Schellmann, Y. Jaillais, J. Friml, B.-H. Kang, Y. Dagdas, Journal of Cell Biology 221 (2022)."},"has_accepted_license":"1","abstract":[{"lang":"eng","text":"Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole is disrupted. CFS1’s function is evolutionarily conserved in plants, as it also localizes to the autophagosomes and plays a role in autophagic flux in the liverwort Marchantia polymorpha. CFS1 regulates autophagic flux by bridging autophagosomes with the multivesicular body-localized ESCRT-I component VPS23A, leading to the formation of amphisomes. Similar to CFS1-ATG8 interaction, disrupting the CFS1-VPS23A interaction blocks autophagic flux and renders plants sensitive to nitrogen starvation. Altogether, our results reveal a conserved vacuolar sorting hub that regulates autophagic flux in plants."}],"status":"public","issue":"12","file":[{"relation":"main_file","checksum":"050b5cc4b25e6b94fe3e3cbfe0f5c06b","date_created":"2023-01-23T10:30:11Z","success":1,"content_type":"application/pdf","creator":"dernst","file_size":10365777,"file_name":"2022_JCB_Zhao.pdf","date_updated":"2023-01-23T10:30:11Z","file_id":"12342","access_level":"open_access"}],"publication":"Journal of Cell Biology","oa_version":"Published Version","article_processing_charge":"No","doi":"10.1083/jcb.202203139","volume":221,"date_published":"2022-12-01T00:00:00Z","language":[{"iso":"eng"}],"file_date_updated":"2023-01-23T10:30:11Z","isi":1,"day":"01","date_updated":"2023-08-03T14:20:15Z","type":"journal_article","publisher":"Rockefeller University Press","author":[{"first_name":"Jierui","last_name":"Zhao","full_name":"Zhao, Jierui"},{"last_name":"Bui","full_name":"Bui, Mai Thu","first_name":"Mai Thu"},{"first_name":"Juncai","last_name":"Ma","full_name":"Ma, Juncai"},{"first_name":"Fabian","last_name":"Künzl","full_name":"Künzl, Fabian"},{"last_name":"Picchianti","full_name":"Picchianti, Lorenzo","first_name":"Lorenzo"},{"first_name":"Juan Carlos","full_name":"De La Concepcion, Juan Carlos","last_name":"De La Concepcion"},{"first_name":"Yixuan","full_name":"Chen, Yixuan","last_name":"Chen"},{"first_name":"Sofia","last_name":"Petsangouraki","full_name":"Petsangouraki, Sofia"},{"first_name":"Azadeh","full_name":"Mohseni, Azadeh","last_name":"Mohseni"},{"last_name":"García-Leon","full_name":"García-Leon, Marta","first_name":"Marta"},{"first_name":"Marta Salas","last_name":"Gomez","full_name":"Gomez, Marta Salas"},{"last_name":"Giannini","full_name":"Giannini, Caterina","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","first_name":"Caterina"},{"last_name":"Gwennogan","full_name":"Gwennogan, Dubois","first_name":"Dubois"},{"full_name":"Kobylinska, Roksolana","last_name":"Kobylinska","first_name":"Roksolana"},{"full_name":"Clavel, Marion","last_name":"Clavel","first_name":"Marion"},{"full_name":"Schellmann, Swen","last_name":"Schellmann","first_name":"Swen"},{"full_name":"Jaillais, Yvon","last_name":"Jaillais","first_name":"Yvon"},{"orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří"},{"last_name":"Kang","full_name":"Kang, Byung-Ho","first_name":"Byung-Ho"},{"first_name":"Yasin","full_name":"Dagdas, Yasin","last_name":"Dagdas"}]},{"type":"journal_article","publisher":"Rockefeller University Press","author":[{"first_name":"Ann-Kathrin","last_name":"Weier","full_name":"Weier, Ann-Kathrin"},{"last_name":"Homrich","full_name":"Homrich, Mirka","first_name":"Mirka"},{"last_name":"Ebbinghaus","full_name":"Ebbinghaus, Stephanie","first_name":"Stephanie"},{"full_name":"Juda, Pavel","last_name":"Juda","first_name":"Pavel"},{"last_name":"Miková","full_name":"Miková, Eliška","first_name":"Eliška"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"last_name":"Zhang","full_name":"Zhang, Lili","first_name":"Lili"},{"first_name":"Thomas","full_name":"Quast, Thomas","last_name":"Quast"},{"last_name":"Mass","full_name":"Mass, Elvira","first_name":"Elvira"},{"last_name":"Schlitzer","full_name":"Schlitzer, Andreas","first_name":"Andreas"},{"full_name":"Kolanus, Waldemar","last_name":"Kolanus","first_name":"Waldemar"},{"last_name":"Burgdorf","full_name":"Burgdorf, Sven","first_name":"Sven"},{"first_name":"Oliver J.","last_name":"Gruß","full_name":"Gruß, Oliver J."},{"first_name":"Miroslav","last_name":"Hons","full_name":"Hons, Miroslav"},{"last_name":"Wieser","full_name":"Wieser, Stefan","first_name":"Stefan"},{"full_name":"Kiermaier, Eva","last_name":"Kiermaier","first_name":"Eva"}],"project":[{"grant_number":"CZI01","name":"Tools for automation and feedback microscopy","_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473"}],"isi":1,"day":"05","date_updated":"2025-04-15T08:37:41Z","doi":"10.1083/jcb.202107134","article_processing_charge":"No","volume":221,"date_published":"2022-12-05T00:00:00Z","language":[{"iso":"eng"}],"file_date_updated":"2023-08-16T11:24:53Z","abstract":[{"lang":"eng","text":"Centrosomes play a crucial role during immune cell interactions and initiation of the immune response. In proliferating cells, centrosome numbers are tightly controlled and generally limited to one in G1 and two prior to mitosis. Defects in regulating centrosome numbers have been associated with cell transformation and tumorigenesis. Here, we report the emergence of extra centrosomes in leukocytes during immune activation. Upon antigen encounter, dendritic cells pass through incomplete mitosis and arrest in the subsequent G1 phase leading to tetraploid cells with accumulated centrosomes. In addition, cell stimulation increases expression of polo-like kinase 2, resulting in diploid cells with two centrosomes in G1-arrested cells. During cell migration, centrosomes tightly cluster and act as functional microtubule-organizing centers allowing for increased persistent locomotion along gradients of chemotactic cues. Moreover, dendritic cells with extra centrosomes display enhanced secretion of inflammatory cytokines and optimized T cell responses. Together, these results demonstrate a previously unappreciated role of extra centrosomes for regular cell and tissue homeostasis."}],"status":"public","issue":"12","publication":"Journal of Cell Biology","file":[{"access_level":"open_access","file_name":"2023_JCB_Weier.pdf","date_updated":"2023-08-16T11:24:53Z","file_id":"14065","file_size":11090179,"creator":"dernst","content_type":"application/pdf","success":1,"checksum":"0c9af38f82af30c6ce528f2caece4246","date_created":"2023-08-16T11:24:53Z","relation":"main_file"}],"oa_version":"Published Version","date_created":"2023-01-12T12:01:09Z","title":"Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells","quality_controlled":"1","department":[{"_id":"Bio"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","image":"/images/cc_by_nc_sa.png","short":"CC BY-NC-SA (4.0)"},"citation":{"mla":"Weier, Ann-Kathrin, et al. “Multiple Centrosomes Enhance Migration and Immune Cell Effector Functions of Mature Dendritic Cells.” <i>Journal of Cell Biology</i>, vol. 221, no. 12, e202107134, Rockefeller University Press, 2022, doi:<a href=\"https://doi.org/10.1083/jcb.202107134\">10.1083/jcb.202107134</a>.","chicago":"Weier, Ann-Kathrin, Mirka Homrich, Stephanie Ebbinghaus, Pavel Juda, Eliška Miková, Robert Hauschild, Lili Zhang, et al. “Multiple Centrosomes Enhance Migration and Immune Cell Effector Functions of Mature Dendritic Cells.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2022. <a href=\"https://doi.org/10.1083/jcb.202107134\">https://doi.org/10.1083/jcb.202107134</a>.","ieee":"A.-K. Weier <i>et al.</i>, “Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells,” <i>Journal of Cell Biology</i>, vol. 221, no. 12. Rockefeller University Press, 2022.","apa":"Weier, A.-K., Homrich, M., Ebbinghaus, S., Juda, P., Miková, E., Hauschild, R., … Kiermaier, E. (2022). Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202107134\">https://doi.org/10.1083/jcb.202107134</a>","ama":"Weier A-K, Homrich M, Ebbinghaus S, et al. Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells. <i>Journal of Cell Biology</i>. 2022;221(12). doi:<a href=\"https://doi.org/10.1083/jcb.202107134\">10.1083/jcb.202107134</a>","ista":"Weier A-K, Homrich M, Ebbinghaus S, Juda P, Miková E, Hauschild R, Zhang L, Quast T, Mass E, Schlitzer A, Kolanus W, Burgdorf S, Gruß OJ, Hons M, Wieser S, Kiermaier E. 2022. Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells. Journal of Cell Biology. 221(12), e202107134.","short":"A.-K. Weier, M. Homrich, S. Ebbinghaus, P. Juda, E. Miková, R. Hauschild, L. Zhang, T. Quast, E. Mass, A. Schlitzer, W. Kolanus, S. Burgdorf, O.J. Gruß, M. Hons, S. Wieser, E. Kiermaier, Journal of Cell Biology 221 (2022)."},"pmid":1,"has_accepted_license":"1","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"article_number":"e202107134","_id":"12122","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"       221","keyword":["Cell Biology"],"external_id":{"isi":["000932941400001"],"pmid":["36214847 "]},"year":"2022","ddc":["570"],"publication_status":"published","acknowledgement":"We thank Markéta Dalecká and Irena Krejzová for their support with FIB-SEM imaging, the Imaging Methods Core Facility at BIOCEV supported by the Ministry of Education, Youth and Sports Czech Republic (Large RI Project LM2018129 Czech-BioImaging), and European Regional Development Fund (project No. CZ.02.1.01/0.0/0.0/18_046/0016045) for their support with obtaining imaging data presented in this paper. The authors further thank Andreas Villunger, Florian Gärtner, Frank Bradke, and Sarah Förster for helpful discussions; Andy Zielinski for help with statistics; and Björn Weiershausen for assisting with figure illustration.\r\n\r\nThis work was funded by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) to E. Kiermaier and the Deutsche Forschungsgemeinschaft (German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048. R. Hauschild was funded by grant number 2020-225401 from the Chan Zuckerberg Initiative Donor-Advised Fund, an advised fund of Silicon Valley Community Foundation. M. Hons is supported by Czech Science Foundation GACR 20-24603Y and Charles University PRIMUS/20/MED/013.","oa":1,"article_type":"original","scopus_import":"1","month":"12"}]