[{"scopus_import":"1","file":[{"success":1,"checksum":"050b5cc4b25e6b94fe3e3cbfe0f5c06b","creator":"dernst","relation":"main_file","file_id":"12342","access_level":"open_access","file_size":10365777,"content_type":"application/pdf","date_created":"2023-01-23T10:30:11Z","date_updated":"2023-01-23T10:30:11Z","file_name":"2022_JCB_Zhao.pdf"}],"publisher":"Rockefeller University Press","publication_identifier":{"eissn":["1540-8140"],"issn":["0021-9525"]},"article_type":"original","author":[{"last_name":"Zhao","full_name":"Zhao, Jierui","first_name":"Jierui"},{"last_name":"Bui","full_name":"Bui, Mai Thu","first_name":"Mai Thu"},{"full_name":"Ma, Juncai","last_name":"Ma","first_name":"Juncai"},{"first_name":"Fabian","last_name":"Künzl","full_name":"Künzl, Fabian"},{"first_name":"Lorenzo","full_name":"Picchianti, Lorenzo","last_name":"Picchianti"},{"full_name":"De La Concepcion, Juan Carlos","last_name":"De La Concepcion","first_name":"Juan Carlos"},{"first_name":"Yixuan","full_name":"Chen, Yixuan","last_name":"Chen"},{"first_name":"Sofia","last_name":"Petsangouraki","full_name":"Petsangouraki, Sofia"},{"first_name":"Azadeh","last_name":"Mohseni","full_name":"Mohseni, Azadeh"},{"first_name":"Marta","full_name":"García-Leon, Marta","last_name":"García-Leon"},{"first_name":"Marta Salas","full_name":"Gomez, Marta Salas","last_name":"Gomez"},{"first_name":"Caterina","full_name":"Giannini, Caterina","last_name":"Giannini","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4"},{"last_name":"Gwennogan","full_name":"Gwennogan, Dubois","first_name":"Dubois"},{"first_name":"Roksolana","full_name":"Kobylinska, Roksolana","last_name":"Kobylinska"},{"last_name":"Clavel","full_name":"Clavel, Marion","first_name":"Marion"},{"first_name":"Swen","last_name":"Schellmann","full_name":"Schellmann, Swen"},{"full_name":"Jaillais, Yvon","last_name":"Jaillais","first_name":"Yvon"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"first_name":"Byung-Ho","last_name":"Kang","full_name":"Kang, Byung-Ho"},{"first_name":"Yasin","last_name":"Dagdas","full_name":"Dagdas, Yasin"}],"quality_controlled":"1","title":"Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole","date_created":"2023-01-12T11:57:10Z","_id":"12121","file_date_updated":"2023-01-23T10:30:11Z","language":[{"iso":"eng"}],"year":"2022","intvolume":"       221","oa":1,"publication":"Journal of Cell Biology","volume":221,"day":"01","acknowledgement":"We thank Suayip Ustün, Karin Schumacher, Erika Isono, Gerd Juergens, Takashi Ueda, Daniel Hofius, and Liwen Jiang for sharing published materials.\r\nWe acknowledge funding from Austrian Academy of Sciences, Austrian Science Fund (FWF, P 32355, P 34944), Austrian Science Fund (FWF-SFB F79), Vienna Science and Technology\r\nFund (WWTF, LS17-047) to Y. Dagdas; Austrian Academy of Sciences DOC Fellowship to J. Zhao, Marie Curie VIP2 Fellowship to J.C. De La Concepcion and M. Clavel; Hong Kong Research Grant Council (GRF14121019, 14113921, AoE/M-05/12, C4002-17G) to B.-H. Kang. We thank Vienna Biocenter Core Facilities (VBCF) Protein Chemistry, Biooptics, Plant Sciences, Molecular Biology, and Protein Technologies. We thank J. Matthew Watson\r\nand members of the Dagdas lab for the critical reading and editing of the manuscript.","abstract":[{"lang":"eng","text":"Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole is disrupted. CFS1’s function is evolutionarily conserved in plants, as it also localizes to the autophagosomes and plays a role in autophagic flux in the liverwort Marchantia polymorpha. CFS1 regulates autophagic flux by bridging autophagosomes with the multivesicular body-localized ESCRT-I component VPS23A, leading to the formation of amphisomes. Similar to CFS1-ATG8 interaction, disrupting the CFS1-VPS23A interaction blocks autophagic flux and renders plants sensitive to nitrogen starvation. Altogether, our results reveal a conserved vacuolar sorting hub that regulates autophagic flux in plants."}],"month":"12","type":"journal_article","external_id":{"isi":["000932958800001"],"pmid":["36260289"]},"oa_version":"Published Version","citation":{"chicago":"Zhao, Jierui, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti, Juan Carlos De La Concepcion, Yixuan Chen, et al. “Plant Autophagosomes Mature into Amphisomes Prior to Their Delivery to the Central Vacuole.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2022. <a href=\"https://doi.org/10.1083/jcb.202203139\">https://doi.org/10.1083/jcb.202203139</a>.","ama":"Zhao J, Bui MT, Ma J, et al. Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole. <i>Journal of Cell Biology</i>. 2022;221(12). doi:<a href=\"https://doi.org/10.1083/jcb.202203139\">10.1083/jcb.202203139</a>","ista":"Zhao J, Bui MT, Ma J, Künzl F, Picchianti L, De La Concepcion JC, Chen Y, Petsangouraki S, Mohseni A, García-Leon M, Gomez MS, Giannini C, Gwennogan D, Kobylinska R, Clavel M, Schellmann S, Jaillais Y, Friml J, Kang B-H, Dagdas Y. 2022. Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole. Journal of Cell Biology. 221(12), e202203139.","ieee":"J. Zhao <i>et al.</i>, “Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole,” <i>Journal of Cell Biology</i>, vol. 221, no. 12. Rockefeller University Press, 2022.","short":"J. Zhao, M.T. Bui, J. Ma, F. Künzl, L. Picchianti, J.C. De La Concepcion, Y. Chen, S. Petsangouraki, A. Mohseni, M. García-Leon, M.S. Gomez, C. Giannini, D. Gwennogan, R. Kobylinska, M. Clavel, S. Schellmann, Y. Jaillais, J. Friml, B.-H. Kang, Y. Dagdas, Journal of Cell Biology 221 (2022).","apa":"Zhao, J., Bui, M. T., Ma, J., Künzl, F., Picchianti, L., De La Concepcion, J. C., … Dagdas, Y. (2022). Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202203139\">https://doi.org/10.1083/jcb.202203139</a>","mla":"Zhao, Jierui, et al. “Plant Autophagosomes Mature into Amphisomes Prior to Their Delivery to the Central Vacuole.” <i>Journal of Cell Biology</i>, vol. 221, no. 12, e202203139, Rockefeller University Press, 2022, doi:<a href=\"https://doi.org/10.1083/jcb.202203139\">10.1083/jcb.202203139</a>."},"ddc":["580"],"date_published":"2022-12-01T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"doi":"10.1083/jcb.202203139","isi":1,"status":"public","article_number":"e202203139","pmid":1,"department":[{"_id":"JiFr"}],"has_accepted_license":"1","article_processing_charge":"No","fulldoi":"https://doi.org/10.1083/jcb.202203139","date_updated":"2023-08-03T14:20:15Z","publication_status":"published","issue":"12","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","keyword":["Cell Biology"]},{"article_number":"e202107134","pmid":1,"license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","department":[{"_id":"Bio"}],"article_processing_charge":"No","has_accepted_license":"1","issue":"12","publication_status":"published","date_updated":"2025-04-15T08:37:41Z","fulldoi":"https://doi.org/10.1083/jcb.202107134","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","keyword":["Cell Biology"],"abstract":[{"lang":"eng","text":"Centrosomes play a crucial role during immune cell interactions and initiation of the immune response. In proliferating cells, centrosome numbers are tightly controlled and generally limited to one in G1 and two prior to mitosis. Defects in regulating centrosome numbers have been associated with cell transformation and tumorigenesis. Here, we report the emergence of extra centrosomes in leukocytes during immune activation. Upon antigen encounter, dendritic cells pass through incomplete mitosis and arrest in the subsequent G1 phase leading to tetraploid cells with accumulated centrosomes. In addition, cell stimulation increases expression of polo-like kinase 2, resulting in diploid cells with two centrosomes in G1-arrested cells. During cell migration, centrosomes tightly cluster and act as functional microtubule-organizing centers allowing for increased persistent locomotion along gradients of chemotactic cues. Moreover, dendritic cells with extra centrosomes display enhanced secretion of inflammatory cytokines and optimized T cell responses. Together, these results demonstrate a previously unappreciated role of extra centrosomes for regular cell and tissue homeostasis."}],"month":"12","external_id":{"pmid":["36214847 "],"isi":["000932941400001"]},"type":"journal_article","oa_version":"Published Version","citation":{"apa":"Weier, A.-K., Homrich, M., Ebbinghaus, S., Juda, P., Miková, E., Hauschild, R., … Kiermaier, E. (2022). Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202107134\">https://doi.org/10.1083/jcb.202107134</a>","mla":"Weier, Ann-Kathrin, et al. “Multiple Centrosomes Enhance Migration and Immune Cell Effector Functions of Mature Dendritic Cells.” <i>Journal of Cell Biology</i>, vol. 221, no. 12, e202107134, Rockefeller University Press, 2022, doi:<a href=\"https://doi.org/10.1083/jcb.202107134\">10.1083/jcb.202107134</a>.","chicago":"Weier, Ann-Kathrin, Mirka Homrich, Stephanie Ebbinghaus, Pavel Juda, Eliška Miková, Robert Hauschild, Lili Zhang, et al. “Multiple Centrosomes Enhance Migration and Immune Cell Effector Functions of Mature Dendritic Cells.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2022. <a href=\"https://doi.org/10.1083/jcb.202107134\">https://doi.org/10.1083/jcb.202107134</a>.","ama":"Weier A-K, Homrich M, Ebbinghaus S, et al. Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells. <i>Journal of Cell Biology</i>. 2022;221(12). doi:<a href=\"https://doi.org/10.1083/jcb.202107134\">10.1083/jcb.202107134</a>","ista":"Weier A-K, Homrich M, Ebbinghaus S, Juda P, Miková E, Hauschild R, Zhang L, Quast T, Mass E, Schlitzer A, Kolanus W, Burgdorf S, Gruß OJ, Hons M, Wieser S, Kiermaier E. 2022. Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells. Journal of Cell Biology. 221(12), e202107134.","ieee":"A.-K. Weier <i>et al.</i>, “Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells,” <i>Journal of Cell Biology</i>, vol. 221, no. 12. Rockefeller University Press, 2022.","short":"A.-K. Weier, M. Homrich, S. Ebbinghaus, P. Juda, E. Miková, R. Hauschild, L. Zhang, T. Quast, E. Mass, A. Schlitzer, W. Kolanus, S. Burgdorf, O.J. Gruß, M. Hons, S. Wieser, E. Kiermaier, Journal of Cell Biology 221 (2022)."},"ddc":["570"],"date_published":"2022-12-05T00:00:00Z","tmp":{"image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","short":"CC BY-NC-SA (4.0)"},"isi":1,"doi":"10.1083/jcb.202107134","project":[{"_id":"c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473","name":"Tools for automation and feedback microscopy","grant_number":"CZI01"}],"status":"public","language":[{"iso":"eng"}],"year":"2022","intvolume":"       221","oa":1,"volume":221,"publication":"Journal of Cell Biology","acknowledgement":"We thank Markéta Dalecká and Irena Krejzová for their support with FIB-SEM imaging, the Imaging Methods Core Facility at BIOCEV supported by the Ministry of Education, Youth and Sports Czech Republic (Large RI Project LM2018129 Czech-BioImaging), and European Regional Development Fund (project No. CZ.02.1.01/0.0/0.0/18_046/0016045) for their support with obtaining imaging data presented in this paper. The authors further thank Andreas Villunger, Florian Gärtner, Frank Bradke, and Sarah Förster for helpful discussions; Andy Zielinski for help with statistics; and Björn Weiershausen for assisting with figure illustration.\r\n\r\nThis work was funded by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) to E. Kiermaier and the Deutsche Forschungsgemeinschaft (German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048. R. Hauschild was funded by grant number 2020-225401 from the Chan Zuckerberg Initiative Donor-Advised Fund, an advised fund of Silicon Valley Community Foundation. M. Hons is supported by Czech Science Foundation GACR 20-24603Y and Charles University PRIMUS/20/MED/013.","day":"05","file":[{"file_size":11090179,"content_type":"application/pdf","date_created":"2023-08-16T11:24:53Z","date_updated":"2023-08-16T11:24:53Z","file_name":"2023_JCB_Weier.pdf","success":1,"checksum":"0c9af38f82af30c6ce528f2caece4246","creator":"dernst","relation":"main_file","file_id":"14065","access_level":"open_access"}],"scopus_import":"1","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"publisher":"Rockefeller University Press","article_type":"original","author":[{"last_name":"Weier","full_name":"Weier, Ann-Kathrin","first_name":"Ann-Kathrin"},{"full_name":"Homrich, Mirka","last_name":"Homrich","first_name":"Mirka"},{"last_name":"Ebbinghaus","full_name":"Ebbinghaus, Stephanie","first_name":"Stephanie"},{"first_name":"Pavel","last_name":"Juda","full_name":"Juda, Pavel"},{"full_name":"Miková, Eliška","last_name":"Miková","first_name":"Eliška"},{"first_name":"Robert","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"first_name":"Lili","last_name":"Zhang","full_name":"Zhang, Lili"},{"first_name":"Thomas","full_name":"Quast, Thomas","last_name":"Quast"},{"first_name":"Elvira","full_name":"Mass, Elvira","last_name":"Mass"},{"full_name":"Schlitzer, Andreas","last_name":"Schlitzer","first_name":"Andreas"},{"first_name":"Waldemar","last_name":"Kolanus","full_name":"Kolanus, Waldemar"},{"full_name":"Burgdorf, Sven","last_name":"Burgdorf","first_name":"Sven"},{"first_name":"Oliver J.","full_name":"Gruß, Oliver J.","last_name":"Gruß"},{"first_name":"Miroslav","last_name":"Hons","full_name":"Hons, Miroslav"},{"first_name":"Stefan","full_name":"Wieser, Stefan","last_name":"Wieser"},{"full_name":"Kiermaier, Eva","last_name":"Kiermaier","first_name":"Eva"}],"title":"Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells","quality_controlled":"1","file_date_updated":"2023-08-16T11:24:53Z","_id":"12122","date_created":"2023-01-12T12:01:09Z"},{"article_processing_charge":"No","has_accepted_license":"1","department":[{"_id":"CaBe"}],"pmid":1,"keyword":["Cell Biology","Molecular Biology"],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","issue":"21","date_updated":"2023-08-04T08:57:17Z","fulldoi":"https://doi.org/10.1016/j.molcel.2022.10.010","oa_version":"Published Version","external_id":{"pmid":["36332606"],"isi":["000898565300011"]},"type":"journal_article","month":"11","abstract":[{"lang":"eng","text":"MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucleases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is specifically adapted to miRNA biogenesis is unknown. We show that the adaptation entails a unique structural role of Dicer’s DExD/H helicase domain. Although mice tolerate loss of its putative ATPase function, the complete absence of the domain is lethal because it assures high-fidelity miRNA biogenesis. Structures of murine Dicer⋅miRNA precursor complexes revealed that the DExD/H domain has a helicase-unrelated structural function. It locks Dicer in a closed state, which facilitates miRNA precursor selection. Transition to a cleavage-competent open state is stimulated by Dicer-binding protein TARBP2. Absence of the DExD/H domain or its mutations unlocks the closed state, reduces substrate selectivity, and activates RNAi. Thus, the DExD/H domain structurally contributes to mammalian miRNA biogenesis and underlies mechanistical partitioning of miRNA and RNAi pathways."}],"status":"public","doi":"10.1016/j.molcel.2022.10.010","isi":1,"date_published":"2022-11-03T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"citation":{"mla":"Zapletal, David, et al. “Structural and Functional Basis of Mammalian MicroRNA Biogenesis by Dicer.” <i>Molecular Cell</i>, vol. 82, no. 21, Elsevier, 2022, p. 4064–4079.e13, doi:<a href=\"https://doi.org/10.1016/j.molcel.2022.10.010\">10.1016/j.molcel.2022.10.010</a>.","apa":"Zapletal, D., Taborska, E., Pasulka, J., Malik, R., Kubicek, K., Zanova, M., … Svoboda, P. (2022). Structural and functional basis of mammalian microRNA biogenesis by Dicer. <i>Molecular Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molcel.2022.10.010\">https://doi.org/10.1016/j.molcel.2022.10.010</a>","chicago":"Zapletal, David, Eliska Taborska, Josef Pasulka, Radek Malik, Karel Kubicek, Martina Zanova, Christian Much, et al. “Structural and Functional Basis of Mammalian MicroRNA Biogenesis by Dicer.” <i>Molecular Cell</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.molcel.2022.10.010\">https://doi.org/10.1016/j.molcel.2022.10.010</a>.","ama":"Zapletal D, Taborska E, Pasulka J, et al. Structural and functional basis of mammalian microRNA biogenesis by Dicer. <i>Molecular Cell</i>. 2022;82(21):4064-4079.e13. doi:<a href=\"https://doi.org/10.1016/j.molcel.2022.10.010\">10.1016/j.molcel.2022.10.010</a>","ista":"Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O’Carroll D, Stefl R, Svoboda P. 2022. Structural and functional basis of mammalian microRNA biogenesis by Dicer. Molecular Cell. 82(21), 4064–4079.e13.","ieee":"D. Zapletal <i>et al.</i>, “Structural and functional basis of mammalian microRNA biogenesis by Dicer,” <i>Molecular Cell</i>, vol. 82, no. 21. Elsevier, p. 4064–4079.e13, 2022.","short":"D. Zapletal, E. Taborska, J. Pasulka, R. Malik, K. Kubicek, M. Zanova, C. Much, M. Sebesta, V. Buccheri, F. Horvat, I. Jenickova, M. Prochazkova, J. Prochazka, M. Pinkas, J. Novacek, D.F. Joseph, R. Sedlacek, C. Bernecky, D. O’Carroll, R. Stefl, P. Svoboda, Molecular Cell 82 (2022) 4064–4079.e13."},"ddc":["570"],"oa":1,"intvolume":"        82","year":"2022","acknowledged_ssus":[{"_id":"EM-Fac"}],"language":[{"iso":"eng"}],"acknowledgement":"We thank Kristian Vlahovicek (University of Zagreb) for support of bioinformatics analyses and Vladimir Benes (EMBL Sequencing Facility) and Genomics and Bioinformatics Core Facility at the Institute of Molecular Genetics for help with RNA sequencing. The main funding was provided by the Czech Science Foundation (EXPRO grant 20-03950X to P.S. and 22-19896S to R. Stefl). Early stages of the work were supported by European Research Council grants under the European Union’s Horizon 2020 Research and Innovation Programme (grants 647403 to P.S. and 649030 to R. Stefl). V.B., D.F.J., and F.H. were in part supported by PhD student fellowships from the Charles University; this work will be in part fulfilling requirements for a PhD degree as “school work.” Funding of D.Z. included the OP RDE project “Internal Grant Agency of Masaryk University” no. CZ.02.2.69/0.0/0.0/19_073/0016943. The Ministry of Education, Youth, and Sports of the Czech Republic (MEYS CR) provided institutional support for CEITEC 2020 project LQ1601. For technical support, we acknowledge EMBL Monterotondo’s genome engineering and transgenic core facilities, the Czech Centre for Phenogenomics at the Institute of Molecular Genetics (supported by RVO 68378050 from the Czech Academy of Sciences and LM2018126 and CZ.02.1.01/0.0/0.0/18_046/0015861 CCP Infrastructure Upgrade II from MEYS CR), the Cryo-EM and Proteomics Core Facilities (CEITEC, Masaryk University) supported by the CIISB research infrastructure (LM2018127 from MEYS CR), and support from the Scientific Service Units of ISTA through resources from the Electron Microscopy Facility. Computational resources included e-Infrastruktura CZ (LM2018140) and ELIXIR-CZ (LM2018131) projects by MEYS CR and the Croatian National Centres of Research Excellence in Personalized Healthcare (#KK.01.1.1.01.0010) and Data Science and Advanced Cooperative Systems (#KK.01.1.1.01.0009) projects funded by the European Structural and Investment Funds grants.","day":"03","publication":"Molecular Cell","volume":82,"title":"Structural and functional basis of mammalian microRNA biogenesis by Dicer","quality_controlled":"1","article_type":"original","author":[{"first_name":"David","last_name":"Zapletal","full_name":"Zapletal, David"},{"full_name":"Taborska, Eliska","last_name":"Taborska","first_name":"Eliska"},{"last_name":"Pasulka","full_name":"Pasulka, Josef","first_name":"Josef"},{"first_name":"Radek","full_name":"Malik, Radek","last_name":"Malik"},{"last_name":"Kubicek","full_name":"Kubicek, Karel","first_name":"Karel"},{"first_name":"Martina","full_name":"Zanova, Martina","last_name":"Zanova"},{"last_name":"Much","full_name":"Much, Christian","first_name":"Christian"},{"first_name":"Marek","full_name":"Sebesta, Marek","last_name":"Sebesta"},{"first_name":"Valeria","last_name":"Buccheri","full_name":"Buccheri, Valeria"},{"full_name":"Horvat, Filip","last_name":"Horvat","first_name":"Filip"},{"first_name":"Irena","last_name":"Jenickova","full_name":"Jenickova, Irena"},{"full_name":"Prochazkova, Michaela","last_name":"Prochazkova","first_name":"Michaela"},{"first_name":"Jan","last_name":"Prochazka","full_name":"Prochazka, Jan"},{"first_name":"Matyas","full_name":"Pinkas, Matyas","last_name":"Pinkas"},{"full_name":"Novacek, Jiri","last_name":"Novacek","first_name":"Jiri"},{"first_name":"Diego F.","full_name":"Joseph, Diego F.","last_name":"Joseph"},{"first_name":"Radislav","last_name":"Sedlacek","full_name":"Sedlacek, Radislav"},{"last_name":"Bernecky","full_name":"Bernecky, Carrie A","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","first_name":"Carrie A","orcid":"0000-0003-0893-7036"},{"full_name":"O’Carroll, Dónal","last_name":"O’Carroll","first_name":"Dónal"},{"first_name":"Richard","full_name":"Stefl, Richard","last_name":"Stefl"},{"full_name":"Svoboda, Petr","last_name":"Svoboda","first_name":"Petr"}],"publication_identifier":{"issn":["1097-2765"]},"publisher":"Elsevier","file":[{"content_type":"application/pdf","date_created":"2023-01-24T09:29:02Z","date_updated":"2023-01-24T09:29:02Z","file_name":"2022_MolecularCell_Zapletal.pdf","file_size":7368534,"success":1,"checksum":"999e443b54e4fdaa2542ca5a97619731","file_id":"12354","access_level":"open_access","relation":"main_file","creator":"dernst"}],"scopus_import":"1","file_date_updated":"2023-01-24T09:29:02Z","_id":"12143","page":"4064-4079.e13","date_created":"2023-01-12T12:05:36Z"},{"keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","issue":"19","date_updated":"2026-06-18T17:25:21Z","fulldoi":"https://doi.org/10.1016/j.devcel.2022.09.003","article_processing_charge":"No","department":[{"_id":"CaHe"}],"pmid":1,"status":"public","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2022.09.003"}],"isi":1,"doi":"10.1016/j.devcel.2022.09.003","OA_type":"free access","date_published":"2022-10-01T00:00:00Z","citation":{"apa":"Hino, N., Matsuda, K., Jikko, Y., Maryu, G., Sakai, K., Imamura, R., … Matsuda, M. (2022). A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2022.09.003\">https://doi.org/10.1016/j.devcel.2022.09.003</a>","mla":"Hino, Naoya, et al. “A Feedback Loop between Lamellipodial Extension and HGF-ERK Signaling Specifies Leader Cells during Collective Cell Migration.” <i>Developmental Cell</i>, vol. 57, no. 19, Elsevier, 2022, p. 2290–2304.e7, doi:<a href=\"https://doi.org/10.1016/j.devcel.2022.09.003\">10.1016/j.devcel.2022.09.003</a>.","chicago":"Hino, Naoya, Kimiya Matsuda, Yuya Jikko, Gembu Maryu, Katsuya Sakai, Ryu Imamura, Shinya Tsukiji, et al. “A Feedback Loop between Lamellipodial Extension and HGF-ERK Signaling Specifies Leader Cells during Collective Cell Migration.” <i>Developmental Cell</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.devcel.2022.09.003\">https://doi.org/10.1016/j.devcel.2022.09.003</a>.","ista":"Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, Tsukiji S, Aoki K, Terai K, Hirashima T, Trepat X, Matsuda M. 2022. A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. Developmental Cell. 57(19), 2290–2304.e7.","ama":"Hino N, Matsuda K, Jikko Y, et al. A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. <i>Developmental Cell</i>. 2022;57(19):2290-2304.e7. doi:<a href=\"https://doi.org/10.1016/j.devcel.2022.09.003\">10.1016/j.devcel.2022.09.003</a>","ieee":"N. Hino <i>et al.</i>, “A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration,” <i>Developmental Cell</i>, vol. 57, no. 19. Elsevier, p. 2290–2304.e7, 2022.","short":"N. Hino, K. Matsuda, Y. Jikko, G. Maryu, K. Sakai, R. Imamura, S. Tsukiji, K. Aoki, K. Terai, T. Hirashima, X. Trepat, M. Matsuda, Developmental Cell 57 (2022) 2290–2304.e7."},"ddc":["570"],"oa_version":"Published Version","type":"journal_article","external_id":{"pmid":["36174555"],"isi":["000898428700006"]},"month":"10","abstract":[{"text":"Upon the initiation of collective cell migration, the cells at the free edge are specified as leader cells; however, the mechanism underlying the leader cell specification remains elusive. Here, we show that lamellipodial extension after the release from mechanical confinement causes sustained extracellular signal-regulated kinase (ERK) activation and underlies the leader cell specification. Live-imaging of Madin-Darby canine kidney (MDCK) cells and mouse epidermis through the use of Förster resonance energy transfer (FRET)-based biosensors showed that leader cells exhibit sustained ERK activation in a hepatocyte growth factor (HGF)-dependent manner. Meanwhile, follower cells exhibit oscillatory ERK activation waves in an epidermal growth factor (EGF) signaling-dependent manner. Lamellipodial extension at the free edge increases the cellular sensitivity to HGF. The HGF-dependent ERK activation, in turn, promotes lamellipodial extension, thereby forming a positive feedback loop between cell extension and ERK activation and specifying the cells at the free edge as the leader cells. Our findings show that the integration of physical and biochemical cues underlies the leader cell specification during collective cell migration.","lang":"eng"}],"acknowledgement":"We thank the members of the Matsuda Laboratory for their helpful discussion and encouragement, and we thank K. Hirano and K. Takakura for their technical assistance. This work was supported by the Kyoto University Live Imaging Center. Financial support was provided in the form of JSPS KAKENHI grants (nos. 17J02107 and 20K22653 to N.H., and 20H05898 and 19H00993 to M.M.), a JST CREST grant (no. JPMJCR1654 to M.M.), a Moonshot R&D grant (no. JPMJPS2022-11 to M.M.), Generalitat de Catalunya and the CERCA Programme (no. SGR-2017-01602 to X.T.), MICCINN/FEDER (no. PGC2018-099645-B-I00 to X.T.), and European Research Council (no. Adv-883739 to X.T.). IBEC is a recipient of a Severo Ochoa Award of Excellence from the MINECO. This work was partly supported by an Extramural Collaborative Research Grant of Cancer Research Institute, Kanazawa University.","day":"01","publication":"Developmental Cell","volume":57,"oa":1,"intvolume":"        57","year":"2022","OA_place":"publisher","corr_author":"1","language":[{"iso":"eng"}],"page":"2290-2304.e7","_id":"12238","date_created":"2023-01-16T09:51:39Z","title":"A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration","quality_controlled":"1","article_type":"original","author":[{"first_name":"Naoya","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","last_name":"Hino","full_name":"Hino, Naoya"},{"first_name":"Kimiya","full_name":"Matsuda, Kimiya","last_name":"Matsuda"},{"last_name":"Jikko","full_name":"Jikko, Yuya","first_name":"Yuya"},{"first_name":"Gembu","full_name":"Maryu, Gembu","last_name":"Maryu"},{"first_name":"Katsuya","last_name":"Sakai","full_name":"Sakai, Katsuya"},{"first_name":"Ryu","full_name":"Imamura, Ryu","last_name":"Imamura"},{"first_name":"Shinya","last_name":"Tsukiji","full_name":"Tsukiji, Shinya"},{"full_name":"Aoki, Kazuhiro","last_name":"Aoki","first_name":"Kazuhiro"},{"first_name":"Kenta","full_name":"Terai, Kenta","last_name":"Terai"},{"first_name":"Tsuyoshi","last_name":"Hirashima","full_name":"Hirashima, Tsuyoshi"},{"first_name":"Xavier","last_name":"Trepat","full_name":"Trepat, Xavier"},{"first_name":"Michiyuki","last_name":"Matsuda","full_name":"Matsuda, Michiyuki"}],"publication_identifier":{"issn":["1534-5807"]},"publisher":"Elsevier","scopus_import":"1"},{"department":[{"_id":"SiHi"}],"has_accepted_license":"1","article_processing_charge":"No","article_number":"259234","pmid":1,"keyword":["Cell Biology"],"date_updated":"2023-08-04T10:28:34Z","fulldoi":"https://doi.org/10.1242/jcs.259234","publication_status":"published","issue":"7","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"isi":["000783840400010"],"pmid":["35383828"]},"type":"journal_article","oa_version":"Published Version","abstract":[{"text":"Neurons extend axons to form the complex circuitry of the mature brain. This depends on the coordinated response and continuous remodelling of the microtubule and F-actin networks in the axonal growth cone. Growth cone architecture remains poorly understood at nanoscales. We therefore investigated mouse hippocampal neuron growth cones using cryo-electron tomography to directly visualise their three-dimensional subcellular architecture with molecular detail. Our data showed that the hexagonal arrays of actin bundles that form filopodia penetrate and terminate deep within the growth cone interior. We directly observed the modulation of these and other growth cone actin bundles by alteration of individual F-actin helical structures. Microtubules with blunt, slightly flared or gently curved ends predominated in the growth cone, frequently contained lumenal particles and exhibited lattice defects. Investigation of the effect of absence of doublecortin, a neurodevelopmental cytoskeleton regulator, on growth cone cytoskeleton showed no major anomalies in overall growth cone organisation or in F-actin subpopulations. However, our data suggested that microtubules sustained more structural defects, highlighting the importance of microtubule integrity during growth cone migration.","lang":"eng"}],"month":"04","isi":1,"doi":"10.1242/jcs.259234","status":"public","citation":{"apa":"Atherton, J., Stouffer, M. A., Francis, F., &#38; Moores, C. A. (2022). Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.259234\">https://doi.org/10.1242/jcs.259234</a>","mla":"Atherton, Joseph, et al. “Visualising the Cytoskeletal Machinery in Neuronal Growth Cones Using Cryo-Electron Tomography.” <i>Journal of Cell Science</i>, vol. 135, no. 7, 259234, The Company of Biologists, 2022, doi:<a href=\"https://doi.org/10.1242/jcs.259234\">10.1242/jcs.259234</a>.","ieee":"J. Atherton, M. A. Stouffer, F. Francis, and C. A. Moores, “Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography,” <i>Journal of Cell Science</i>, vol. 135, no. 7. The Company of Biologists, 2022.","short":"J. Atherton, M.A. Stouffer, F. Francis, C.A. Moores, Journal of Cell Science 135 (2022).","chicago":"Atherton, Joseph, Melissa A Stouffer, Fiona Francis, and Carolyn A. Moores. “Visualising the Cytoskeletal Machinery in Neuronal Growth Cones Using Cryo-Electron Tomography.” <i>Journal of Cell Science</i>. The Company of Biologists, 2022. <a href=\"https://doi.org/10.1242/jcs.259234\">https://doi.org/10.1242/jcs.259234</a>.","ama":"Atherton J, Stouffer MA, Francis F, Moores CA. Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. <i>Journal of Cell Science</i>. 2022;135(7). doi:<a href=\"https://doi.org/10.1242/jcs.259234\">10.1242/jcs.259234</a>","ista":"Atherton J, Stouffer MA, Francis F, Moores CA. 2022. Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. Journal of Cell Science. 135(7), 259234."},"ddc":["570"],"date_published":"2022-04-01T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"       135","oa":1,"language":[{"iso":"eng"}],"year":"2022","day":"01","acknowledgement":"J.A. was supported by a grant from the Medical Research Council (MRC), UK (MR/R000352/1) to C.A.M. Cryo-EM data were collected on equipment funded by the Wellcome Trust, UK (079605/Z/06/Z) and the Biotechnology and Biological Sciences Research Council (BBSRC) UK (BB/L014211/1). F.F.’s salary and institute were supported by Inserm (Institut National de la Santé et de la Recherche Médicale), CNRS (Centre National de la Recherche Scientifique) and Sorbonne Université. F.F.’s group was particularly supported by Agence Nationale de la\r\nRecherche (ANR-16-CE16-0011-03) and Seventh Framework Programme (EUHEALTH-\r\n2013, DESIRE, N° 60253; also funding M.S.’s salary) and the European Cooperation in Science and Technology (COST Action CA16118). Open Access funding provided by Birkbeck College: Birkbeck University of London. Deposited in PMC for immediate release.","volume":135,"publication":"Journal of Cell Science","author":[{"first_name":"Joseph","last_name":"Atherton","full_name":"Atherton, Joseph"},{"first_name":"Melissa A","id":"4C9372C4-F248-11E8-B48F-1D18A9856A87","last_name":"Stouffer","full_name":"Stouffer, Melissa A"},{"first_name":"Fiona","full_name":"Francis, Fiona","last_name":"Francis"},{"first_name":"Carolyn A.","full_name":"Moores, Carolyn A.","last_name":"Moores"}],"article_type":"original","quality_controlled":"1","title":"Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography","scopus_import":"1","file":[{"file_size":13868733,"content_type":"application/pdf","file_name":"2022_JourCellBiology_Atherton.pdf","date_created":"2023-01-30T11:41:01Z","date_updated":"2023-01-30T11:41:01Z","relation":"main_file","creator":"dernst","file_id":"12461","access_level":"open_access","success":1,"checksum":"4346ed32cb7c89a8ca051c7da68a9a1c"}],"publisher":"The Company of Biologists","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"date_created":"2023-01-16T10:03:24Z","_id":"12283","file_date_updated":"2023-01-30T11:41:01Z"},{"keyword":["Bacterial cell division","in vitro reconstitution","FtsZ","FtsN","FtsA"],"fulldoi":"https://doi.org/10.15479/AT:ISTA:10934","date_updated":"2026-10-24T22:30:12Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ec_funded":1,"department":[{"_id":"GradSch"},{"_id":"MaLo"}],"has_accepted_license":"1","article_processing_charge":"No","doi":"10.15479/AT:ISTA:10934","project":[{"name":"Self-Organization of the Bacterial Cell","grant_number":"679239","call_identifier":"H2020","_id":"2595697A-B435-11E9-9278-68D0E5697425"},{"grant_number":"P34607","name":"In vitro reconstitution of bacterial cell division","_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d"}],"status":"public","citation":{"ieee":"P. Radler, “In vitro reconstitution of Escherichia coli divisome activation.” Institute of Science and Technology Austria, 2022.","short":"P. Radler, (2022).","ista":"Radler P. 2022. In vitro reconstitution of Escherichia coli divisome activation, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:10934\">10.15479/AT:ISTA:10934</a>.","ama":"Radler P. In vitro reconstitution of Escherichia coli divisome activation. 2022. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:10934\">10.15479/AT:ISTA:10934</a>","chicago":"Radler, Philipp. “In Vitro Reconstitution of Escherichia Coli Divisome Activation.” Institute of Science and Technology Austria, 2022. <a href=\"https://doi.org/10.15479/AT:ISTA:10934\">https://doi.org/10.15479/AT:ISTA:10934</a>.","mla":"Radler, Philipp. <i>In Vitro Reconstitution of Escherichia Coli Divisome Activation</i>. Institute of Science and Technology Austria, 2022, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:10934\">10.15479/AT:ISTA:10934</a>.","apa":"Radler, P. (2022). In vitro reconstitution of Escherichia coli divisome activation. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:10934\">https://doi.org/10.15479/AT:ISTA:10934</a>"},"ddc":["572"],"date_published":"2022-04-05T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"type":"research_data","oa_version":"Submitted Version","related_material":{"record":[{"id":"11373","status":"public","relation":"used_in_publication"},{"status":"public","id":"14280","relation":"used_in_publication"}],"link":[{"url":"https://doi.org/10.5281/zenodo.6400639","relation":"software","description":"A custom written code (FRAPdiff) to quantify the Off binding rate and Diffusion coefficient of membrane bound proteins. Written by Christoph Sommer."}]},"abstract":[{"text":"FtsA is crucial for assembly of the E. coli divisome, as it dynamically links cytoplasmic FtsZ filaments with transmembrane cell division proteins. FtsA allegedly initiates cell division by switching from an inactive polymeric to an active monomeric confirmation, which recruits downstream proteins and stabilizes FtsZ filaments. Here, we use biochemical reconstitution experiments combined with quantitative fluorescence microscopy to study divisome activation in vitro. We compare wildtype-FtsA with FtsA-R286W, a constantly active gain-of-function mutant and find that R286W outperforms the wildtype protein in replicating FtsZ treadmilling dynamics, stabilizing FtsZ filaments and recruiting FtsN. We attribute these differences to a faster membrane exchange of FtsA-R286W and its higher packing density below FtsZ filaments.  Using FRET microscopy, we find that FtsN binding does not compete with, but promotes FtsA self-interaction. Our findings suggest a model where FtsA always forms dynamic polymers on the membrane, which re-organize during assembly and activation of the divisome. ","lang":"eng"}],"month":"04","day":"05","acknowledgement":"We acknowledge members of the Loose laboratory at IST Austria for helpful discussions—in particular L. Lindorfer for his assistance with cloning and purifications. We thank J. Löwe and T. Nierhaus (MRC-LMB Cambridge, UK) for sharing unpublished work and helpful discussions, as well as D. Vavylonis and D. Rutkowski (Lehigh University, Bethlehem, PA, USA) as well as S. Martin (University of Lausanne, Switzerland) for sharing their code for FRAP analysis. We are also thankful for the support by the Scientific Service Units (SSU) of IST Austria through resources provided by the Imaging and Optics Facility (IOF) and the Lab Support Facility (LSF). This work was supported by the European Research Council through grant ERC 2015-StG-679239 and by the Austrian Science Fund (FWF) StandAlone P34607 to M.L. and HFSP LT 000824/2016-L4 to N.B. For the purpose of open access, we have applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.","oa":1,"contributor":[{"id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose","contributor_type":"supervisor","first_name":"Martin","orcid":"0000-0001-7309-9724"},{"last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph M","contributor_type":"researcher"},{"first_name":"Paulo","contributor_type":"researcher","last_name":"Caldas"},{"contributor_type":"researcher","first_name":"David","last_name":"Michalik","id":"B9577E20-AA38-11E9-AC9A-0930E6697425"},{"first_name":"Natalia","contributor_type":"researcher","last_name":"Baranova"}],"corr_author":"1","year":"2022","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"_id":"10934","date_created":"2022-03-31T11:32:32Z","file_date_updated":"2022-04-22T10:15:19Z","author":[{"orcid":" 0000-0001-9198-2182 ","first_name":"Philipp","full_name":"Radler, 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Sixt. “Plan Your Trip before You Leave: The Neutrophils’ Search-and-Run Journey.” <i>Journal of Cell Biology</i>, vol. 221, no. 8, e202206127, Rockefeller University Press, 2022, doi:<a href=\"https://doi.org/10.1083/jcb.202206127\">10.1083/jcb.202206127</a>.","apa":"Stopp, J. A., &#38; Sixt, M. K. (2022). Plan your trip before you leave: The neutrophils’ search-and-run journey. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202206127\">https://doi.org/10.1083/jcb.202206127</a>","short":"J.A. Stopp, M.K. Sixt, Journal of Cell Biology 221 (2022).","ieee":"J. A. Stopp and M. K. Sixt, “Plan your trip before you leave: The neutrophils’ search-and-run journey,” <i>Journal of Cell Biology</i>, vol. 221, no. 8. Rockefeller University Press, 2022.","chicago":"Stopp, Julian A, and Michael K Sixt. “Plan Your Trip before You Leave: The Neutrophils’ Search-and-Run Journey.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2022. <a href=\"https://doi.org/10.1083/jcb.202206127\">https://doi.org/10.1083/jcb.202206127</a>.","ista":"Stopp JA, Sixt MK. 2022. Plan your trip before you leave: The neutrophils’ search-and-run journey. Journal of Cell Biology. 221(8), e202206127.","ama":"Stopp JA, Sixt MK. Plan your trip before you leave: The neutrophils’ search-and-run journey. <i>Journal of Cell Biology</i>. 2022;221(8). doi:<a href=\"https://doi.org/10.1083/jcb.202206127\">10.1083/jcb.202206127</a>"},"ddc":["570"],"oa_version":"Published Version","external_id":{"pmid":["35856919"],"isi":["000874717200001"]},"type":"journal_article","month":"07","abstract":[{"text":"Reading, interpreting and crawling along gradients of chemotactic cues is one of the most complex questions in cell biology. In this issue, Georgantzoglou et al. (2022. J. Cell. Biol.https://doi.org/10.1083/jcb.202103207) use in vivo models to map the temporal sequence of how neutrophils respond to an acutely arising gradient of chemoattractant.","lang":"eng"}],"related_material":{"record":[{"relation":"dissertation_contains","id":"14697","status":"public"}]},"keyword":["Cell Biology"],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","issue":"8","fulldoi":"https://doi.org/10.1083/jcb.202206127","date_updated":"2026-10-24T22:30:21Z","article_processing_charge":"No","has_accepted_license":"1","department":[{"_id":"MiSi"}],"pmid":1,"article_number":"e202206127","file_date_updated":"2023-01-30T10:39:34Z","date_created":"2023-01-16T10:01:08Z","_id":"12272","title":"Plan your trip before you leave: The neutrophils’ search-and-run journey","quality_controlled":"1","article_type":"original","author":[{"first_name":"Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87","full_name":"Stopp, Julian A","last_name":"Stopp"},{"first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"publisher":"Rockefeller University Press","file":[{"creator":"dernst","relation":"main_file","access_level":"open_access","file_id":"12451","checksum":"6b1620743669679b48b9389bb40f5a11","success":1,"file_size":969969,"file_name":"2022_JourCellBiology_Stopp.pdf","date_updated":"2023-01-30T10:39:34Z","date_created":"2023-01-30T10:39:34Z","content_type":"application/pdf"}],"scopus_import":"1","day":"20","publication":"Journal of Cell Biology","volume":221,"oa":1,"intvolume":"       221","year":"2022","corr_author":"1","language":[{"iso":"eng"}]},{"month":"11","abstract":[{"text":"In order to combat molecular damage, most cellular proteins undergo rapid turnover. We have previously identified large nuclear protein assemblies that can persist for years in post-mitotic tissues and are subject to age-related decline. Here, we report that mitochondria can be long lived in the mouse brain and reveal that specific mitochondrial proteins have half-lives longer than the average proteome. These mitochondrial long-lived proteins (mitoLLPs) are core components of the electron transport chain (ETC) and display increased longevity in respiratory supercomplexes. We find that COX7C, a mitoLLP that forms a stable contact site between complexes I and IV, is required for complex IV and supercomplex assembly. Remarkably, even upon depletion of COX7C transcripts, ETC function is maintained for days, effectively uncoupling mitochondrial function from ongoing transcription of its mitoLLPs. Our results suggest that modulating protein longevity within the ETC is critical for mitochondrial proteome maintenance and the robustness of mitochondrial function.","lang":"eng"}],"oa_version":"None","type":"journal_article","external_id":{"pmid":["34715012"]},"date_published":"2021-11-08T00:00:00Z","citation":{"chicago":"Krishna, Shefali, Rafael Arrojo e Drigo, Juliana S. Capitanio, Ranjan Ramachandra, Mark Ellisman, and Martin Hetzer. “Identification of Long-Lived Proteins in the Mitochondria Reveals Increased Stability of the Electron Transport Chain.” <i>Developmental Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.devcel.2021.10.008\">https://doi.org/10.1016/j.devcel.2021.10.008</a>.","ista":"Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer M. 2021. Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. 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P2952–2965.e9, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.10.008\">10.1016/j.devcel.2021.10.008</a>."},"status":"public","doi":"10.1016/j.devcel.2021.10.008","pmid":1,"article_processing_charge":"No","department":[{"_id":"MaHe"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"21","publication_status":"published","date_updated":"2025-12-15T10:01:56Z","fulldoi":"https://doi.org/10.1016/j.devcel.2021.10.008","keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"publication_identifier":{"issn":["1534-5807"]},"publisher":"Elsevier","scopus_import":"1","title":"Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain","quality_controlled":"1","author":[{"first_name":"Shefali","full_name":"Krishna, Shefali","last_name":"Krishna"},{"first_name":"Rafael","full_name":"Arrojo e Drigo, Rafael","last_name":"Arrojo e Drigo"},{"last_name":"Capitanio","full_name":"Capitanio, Juliana S.","first_name":"Juliana S."},{"full_name":"Ramachandra, Ranjan","last_name":"Ramachandra","first_name":"Ranjan"},{"first_name":"Mark","full_name":"Ellisman, Mark","last_name":"Ellisman"},{"orcid":"0000-0002-2111-992X","first_name":"Martin W","last_name":"HETZER","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","full_name":"HETZER, Martin W"}],"article_type":"original","extern":"1","date_created":"2022-04-07T07:43:14Z","_id":"11052","page":"P2952-2965.e9","year":"2021","language":[{"iso":"eng"}],"intvolume":"        56","volume":56,"publication":"Developmental Cell","day":"08"},{"month":"04","abstract":[{"text":"Remdesivir is a nucleoside analog approved by the US FDA for treatment of COVID-19. Here, we present a 3.9-Å-resolution cryo-EM reconstruction of a remdesivir-stalled RNA-dependent RNA polymerase complex, revealing full incorporation of 3 copies of remdesivir monophosphate (RMP) and a partially incorporated fourth RMP in the active site. The structure reveals that RMP blocks RNA translocation after incorporation of 3 bases following RMP, resulting in delayed chain termination, which can guide the rational design of improved antiviral drugs.","lang":"eng"}],"oa_version":"Preprint","external_id":{"pmid":["33631104"]},"type":"journal_article","date_published":"2021-04-01T00:00:00Z","citation":{"ama":"Bravo JPK, Dangerfield TL, Taylor DW, Johnson KA. Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication. <i>Molecular Cell</i>. 2021;81(7):1548-1552.e4. doi:<a href=\"https://doi.org/10.1016/j.molcel.2021.01.035\">10.1016/j.molcel.2021.01.035</a>","chicago":"Bravo, Jack Peter Kelly, Tyler L. Dangerfield, David W. Taylor, and Kenneth A. 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Elsevier. <a href=\"https://doi.org/10.1016/j.molcel.2021.01.035\">https://doi.org/10.1016/j.molcel.2021.01.035</a>","mla":"Bravo, Jack Peter Kelly, et al. “Remdesivir Is a Delayed Translocation Inhibitor of SARS-CoV-2 Replication.” <i>Molecular Cell</i>, vol. 81, no. 7, Elsevier, 2021, p. 1548–1552.e4, doi:<a href=\"https://doi.org/10.1016/j.molcel.2021.01.035\">10.1016/j.molcel.2021.01.035</a>."},"status":"public","main_file_link":[{"url":"https://doi.org/10.1101/2020.12.14.422718 ","open_access":"1"}],"doi":"10.1016/j.molcel.2021.01.035","pmid":1,"article_processing_charge":"No","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","issue":"7","date_updated":"2024-06-04T06:00:56Z","fulldoi":"https://doi.org/10.1016/j.molcel.2021.01.035","keyword":["Cell Biology","Molecular Biology"],"publication_identifier":{"issn":["1097-2765"]},"publisher":"Elsevier","scopus_import":"1","title":"Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication","quality_controlled":"1","author":[{"last_name":"Bravo","full_name":"Bravo, Jack Peter Kelly","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","orcid":"0000-0003-0456-0753","first_name":"Jack Peter Kelly"},{"last_name":"Dangerfield","full_name":"Dangerfield, Tyler L.","first_name":"Tyler L."},{"first_name":"David W.","last_name":"Taylor","full_name":"Taylor, David W."},{"last_name":"Johnson","full_name":"Johnson, Kenneth A.","first_name":"Kenneth A."}],"article_type":"original","extern":"1","page":"1548-1552.e4","_id":"15140","date_created":"2024-03-20T10:42:53Z","year":"2021","language":[{"iso":"eng"}],"oa":1,"intvolume":"        81","volume":81,"publication":"Molecular Cell","day":"01"},{"publisher":"Frontiers Media","publication_identifier":{"issn":["2296-634X"]},"scopus_import":"1","file":[{"content_type":"application/pdf","date_created":"2024-04-03T14:08:05Z","date_updated":"2024-04-03T14:08:05Z","file_name":"2021_Frontiers_Deretic.pdf","file_size":7430029,"success":1,"checksum":"f6330b5c6718d6780383c0300fd4ef12","file_id":"15291","access_level":"open_access","creator":"dernst","relation":"main_file"}],"quality_controlled":"1","title":"The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse","author":[{"first_name":"Nikola","last_name":"Deretic","full_name":"Deretic, Nikola"},{"full_name":"Bolger-Munro, Madison","last_name":"Bolger-Munro","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","first_name":"Madison","orcid":"0000-0002-8176-4824"},{"first_name":"Kate","full_name":"Choi, Kate","last_name":"Choi"},{"last_name":"Abraham","full_name":"Abraham, Libin","first_name":"Libin"},{"first_name":"Michael R.","full_name":"Gold, Michael R.","last_name":"Gold"}],"article_type":"original","_id":"15264","date_created":"2024-04-03T07:34:08Z","file_date_updated":"2024-04-03T14:08:05Z","year":"2021","language":[{"iso":"eng"}],"oa":1,"intvolume":"         9","publication":"Frontiers in Cell and Developmental Biology","volume":9,"day":"01","month":"07","abstract":[{"lang":"eng","text":"Signaling by the B cell antigen receptor (BCR) initiates actin remodeling. The assembly of branched actin networks that are nucleated by the Arp2/3 complex exert outward force on the plasma membrane, allowing B cells to form membrane protrusions that can scan the surface of antigen-presenting cells (APCs). The resulting Arp2/3 complex-dependent actin retrograde flow promotes the centripetal movement and progressive coalescence of BCR microclusters, which amplifies BCR signaling. Glia maturation factor γ (GMFγ) is an actin disassembly-protein that releases Arp2/3 complex-nucleated actin filaments from actin networks. By doing so, GMFγ could either oppose the actions of the Arp2/3 complex or support Arp2/3 complex-nucleated actin polymerization by contributing to the recycling of actin monomers and Arp2/3 complexes. We now show that reducing the levels of GMFγ in human B cell lines via transfection with a specific siRNA impairs the ability of B cells to spread on antigen-coated surfaces, decreases the velocity of actin retrograde flow, diminishes the coalescence of BCR microclusters into a central cluster at the B cell-APC contact site, and decreases APC-induced BCR signaling. These effects of depleting GMFγ are similar to what occurs when the Arp2/3 complex is inhibited. This suggests that GMFγ cooperates with the Arp2/3 complex to support BCR-induced actin remodeling and amplify BCR signaling at the immune synapse."}],"oa_version":"Published Version","external_id":{"pmid":["34336818"]},"type":"journal_article","date_published":"2021-07-01T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"ddc":["570"],"citation":{"short":"N. Deretic, M. Bolger-Munro, K. Choi, L. Abraham, M.R. Gold, Frontiers in Cell and Developmental Biology 9 (2021).","ieee":"N. Deretic, M. Bolger-Munro, K. Choi, L. Abraham, and M. R. Gold, “The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse,” <i>Frontiers in Cell and Developmental Biology</i>, vol. 9. Frontiers Media, 2021.","ista":"Deretic N, Bolger-Munro M, Choi K, Abraham L, Gold MR. 2021. The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse. Frontiers in Cell and Developmental Biology. 9, 647063.","ama":"Deretic N, Bolger-Munro M, Choi K, Abraham L, Gold MR. The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse. <i>Frontiers in Cell and Developmental Biology</i>. 2021;9. doi:<a href=\"https://doi.org/10.3389/fcell.2021.647063\">10.3389/fcell.2021.647063</a>","chicago":"Deretic, Nikola, Madison Bolger-Munro, Kate Choi, Libin Abraham, and Michael R. Gold. “The Actin-Disassembly Protein Glia Maturation Factor γ Enhances Actin Remodeling and B Cell Antigen Receptor Signaling at the Immune Synapse.” <i>Frontiers in Cell and Developmental Biology</i>. Frontiers Media, 2021. <a href=\"https://doi.org/10.3389/fcell.2021.647063\">https://doi.org/10.3389/fcell.2021.647063</a>.","apa":"Deretic, N., Bolger-Munro, M., Choi, K., Abraham, L., &#38; Gold, M. R. (2021). The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse. <i>Frontiers in Cell and Developmental Biology</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fcell.2021.647063\">https://doi.org/10.3389/fcell.2021.647063</a>","mla":"Deretic, Nikola, et al. “The Actin-Disassembly Protein Glia Maturation Factor γ Enhances Actin Remodeling and B Cell Antigen Receptor Signaling at the Immune Synapse.” <i>Frontiers in Cell and Developmental Biology</i>, vol. 9, 647063, Frontiers Media, 2021, doi:<a href=\"https://doi.org/10.3389/fcell.2021.647063\">10.3389/fcell.2021.647063</a>."},"status":"public","doi":"10.3389/fcell.2021.647063","pmid":1,"article_number":"647063","has_accepted_license":"1","article_processing_charge":"No","department":[{"_id":"CaHe"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-04-03T14:10:25Z","fulldoi":"https://doi.org/10.3389/fcell.2021.647063","publication_status":"published","keyword":["Cell Biology","Developmental Biology"]},{"keyword":["cell biology"],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","fulldoi":"https://doi.org/10.1083/jcb.202009154","date_updated":"2021-11-25T15:33:08Z","publication_status":"published","issue":"6","article_processing_charge":"No","pmid":1,"article_number":"e202009154","status":"public","doi":"10.1083/jcb.202009154","date_published":"2021-04-30T00:00:00Z","tmp":{"image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","short":"CC BY-NC-SA (4.0)"},"citation":{"ieee":"L. Zeng, I. Palaia, A. Šarić, and X. Su, “PLCγ1 promotes phase separation of T cell signaling components,” <i>Journal of Cell Biology</i>, vol. 220, no. 6. Rockefeller University Press, 2021.","short":"L. Zeng, I. Palaia, A. Šarić, X. Su, Journal of Cell Biology 220 (2021).","ama":"Zeng L, Palaia I, Šarić A, Su X. PLCγ1 promotes phase separation of T cell signaling components. <i>Journal of Cell Biology</i>. 2021;220(6). doi:<a href=\"https://doi.org/10.1083/jcb.202009154\">10.1083/jcb.202009154</a>","ista":"Zeng L, Palaia I, Šarić A, Su X. 2021. PLCγ1 promotes phase separation of T cell signaling components. Journal of Cell Biology. 220(6), e202009154.","chicago":"Zeng, Longhui, Ivan Palaia, Anđela Šarić, and Xiaolei Su. “PLCγ1 Promotes Phase Separation of T Cell Signaling Components.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2021. <a href=\"https://doi.org/10.1083/jcb.202009154\">https://doi.org/10.1083/jcb.202009154</a>.","mla":"Zeng, Longhui, et al. “PLCγ1 Promotes Phase Separation of T Cell Signaling Components.” <i>Journal of Cell Biology</i>, vol. 220, no. 6, e202009154, Rockefeller University Press, 2021, doi:<a href=\"https://doi.org/10.1083/jcb.202009154\">10.1083/jcb.202009154</a>.","apa":"Zeng, L., Palaia, I., Šarić, A., &#38; Su, X. (2021). PLCγ1 promotes phase separation of T cell signaling components. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202009154\">https://doi.org/10.1083/jcb.202009154</a>"},"oa_version":"None","type":"journal_article","external_id":{"pmid":["33929486"]},"month":"04","abstract":[{"text":"The T cell receptor (TCR) pathway receives, processes, and amplifies the signal from pathogenic antigens to the activation of T cells. Although major components in this pathway have been identified, the knowledge on how individual components cooperate to effectively transduce signals remains limited. Phase separation emerges as a biophysical principle in organizing signaling molecules into liquid-like condensates. Here, we report that phospholipase Cγ1 (PLCγ1) promotes phase separation of LAT, a key adaptor protein in the TCR pathway. PLCγ1 directly cross-links LAT through its two SH2 domains. PLCγ1 also protects LAT from dephosphorylation by the phosphatase CD45 and promotes LAT-dependent ERK activation and SLP76 phosphorylation. Intriguingly, a nonmonotonic effect of PLCγ1 on LAT clustering was discovered. Computer simulations, based on patchy particles, revealed how the cluster size is regulated by protein compositions. Together, these results define a critical function of PLCγ1 in promoting phase separation of the LAT complex and TCR signal transduction.","lang":"eng"}],"day":"30","acknowledgement":"Charles H. Hood Foundation (NO AWARD) ; Rally Foundation (NO AWARD)","publication":"Journal of Cell Biology","volume":220,"intvolume":"       220","year":"2021","language":[{"iso":"eng"}],"_id":"10337","date_created":"2021-11-25T15:21:30Z","extern":"1","quality_controlled":"1","title":"PLCγ1 promotes phase separation of T cell signaling components","article_type":"original","author":[{"full_name":"Zeng, Longhui","last_name":"Zeng","first_name":"Longhui"},{"first_name":"Ivan","full_name":"Palaia, Ivan","last_name":"Palaia"},{"first_name":"Anđela","orcid":"0000-0002-7854-2139","full_name":"Šarić, Anđela","last_name":"Šarić","id":"bf63d406-f056-11eb-b41d-f263a6566d8b"},{"first_name":"Xiaolei","last_name":"Su","full_name":"Su, Xiaolei"}],"publisher":"Rockefeller University Press","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"scopus_import":"1"},{"file_date_updated":"2021-08-11T12:30:38Z","_id":"9188","date_created":"2021-02-23T12:31:43Z","publication_identifier":{"issn":["0197-0186"]},"publisher":"Elsevier","file":[{"file_id":"9883","access_level":"open_access","relation":"main_file","creator":"kschuh","success":1,"checksum":"c6d7a40089cd29e289f9b22e75768304","content_type":"application/pdf","date_updated":"2021-08-11T12:30:38Z","date_created":"2021-08-11T12:30:38Z","file_name":"2021_NCI_Pauler.pdf","file_size":7083499}],"scopus_import":"1","title":"Inducible uniparental chromosome disomy to probe genomic imprinting at single-cell level in brain and beyond","quality_controlled":"1","article_type":"original","author":[{"orcid":"0000-0002-7462-0048","first_name":"Florian","full_name":"Pauler, Florian","last_name":"Pauler","id":"48EA0138-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hudson","full_name":"Hudson, Quanah","first_name":"Quanah"},{"first_name":"Susanne","orcid":"0000-0002-7903-3010","id":"2D6B7A9A-F248-11E8-B48F-1D18A9856A87","last_name":"Laukoter","full_name":"Laukoter, Susanne"},{"orcid":"0000-0003-2279-1061","first_name":"Simon","last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon"}],"publication":"Neurochemistry International","volume":145,"acknowledgement":"We thank Melissa Stouffer for critically reading the manuscript. This work was supported by IST Austria institutional funds; NÖ Forschung und Bildung n[f + b] life science call grant (C13-002) to S.H. and the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement 725780 LinPro) to S.H.","day":"01","year":"2021","language":[{"iso":"eng"}],"oa":1,"intvolume":"       145","date_published":"2021-05-01T00:00:00Z","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"ddc":["570"],"citation":{"mla":"Pauler, Florian, et al. “Inducible Uniparental Chromosome Disomy to Probe Genomic Imprinting at Single-Cell Level in Brain and Beyond.” <i>Neurochemistry International</i>, vol. 145, no. 5, 104986, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.neuint.2021.104986\">10.1016/j.neuint.2021.104986</a>.","apa":"Pauler, F., Hudson, Q., Laukoter, S., &#38; Hippenmeyer, S. (2021). Inducible uniparental chromosome disomy to probe genomic imprinting at single-cell level in brain and beyond. <i>Neurochemistry International</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuint.2021.104986\">https://doi.org/10.1016/j.neuint.2021.104986</a>","short":"F. Pauler, Q. Hudson, S. Laukoter, S. Hippenmeyer, Neurochemistry International 145 (2021).","ieee":"F. Pauler, Q. Hudson, S. Laukoter, and S. Hippenmeyer, “Inducible uniparental chromosome disomy to probe genomic imprinting at single-cell level in brain and beyond,” <i>Neurochemistry International</i>, vol. 145, no. 5. Elsevier, 2021.","ama":"Pauler F, Hudson Q, Laukoter S, Hippenmeyer S. Inducible uniparental chromosome disomy to probe genomic imprinting at single-cell level in brain and beyond. <i>Neurochemistry International</i>. 2021;145(5). doi:<a href=\"https://doi.org/10.1016/j.neuint.2021.104986\">10.1016/j.neuint.2021.104986</a>","ista":"Pauler F, Hudson Q, Laukoter S, Hippenmeyer S. 2021. Inducible uniparental chromosome disomy to probe genomic imprinting at single-cell level in brain and beyond. Neurochemistry International. 145(5), 104986.","chicago":"Pauler, Florian, Quanah Hudson, Susanne Laukoter, and Simon Hippenmeyer. “Inducible Uniparental Chromosome Disomy to Probe Genomic Imprinting at Single-Cell Level in Brain and Beyond.” <i>Neurochemistry International</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.neuint.2021.104986\">https://doi.org/10.1016/j.neuint.2021.104986</a>."},"project":[{"name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","grant_number":"725780","_id":"260018B0-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"LS13-002","name":"Mapping Cell-Type Specificity of the Genomic Imprintome in the Brain","_id":"25D92700-B435-11E9-9278-68D0E5697425"}],"status":"public","doi":"10.1016/j.neuint.2021.104986","isi":1,"month":"05","abstract":[{"text":"Genomic imprinting is an epigenetic mechanism that results in parental allele-specific expression of ~1% of all genes in mouse and human. Imprinted genes are key developmental regulators and play pivotal roles in many biological processes such as nutrient transfer from the mother to offspring and neuronal development. Imprinted genes are also involved in human disease, including neurodevelopmental disorders, and often occur in clusters that are regulated by a common imprint control region (ICR). In extra-embryonic tissues ICRs can act over large distances, with the largest surrounding Igf2r spanning over 10 million base-pairs. Besides classical imprinted expression that shows near exclusive maternal or paternal expression, widespread biased imprinted expression has been identified mainly in brain. In this review we discuss recent developments mapping cell type specific imprinted expression in extra-embryonic tissues and neocortex in the mouse. We highlight the advantages of using an inducible uniparental chromosome disomy (UPD) system to generate cells carrying either two maternal or two paternal copies of a specific chromosome to analyze the functional consequences of genomic imprinting. Mosaic Analysis with Double Markers (MADM) allows fluorescent labeling and concomitant induction of UPD sparsely in specific cell types, and thus to over-express or suppress all imprinted genes on that chromosome. To illustrate the utility of this technique, we explain how MADM-induced UPD revealed new insights about the function of the well-studied Cdkn1c imprinted gene, and how MADM-induced UPDs led to identification of highly cell type specific phenotypes related to perturbed imprinted expression in the mouse neocortex. Finally, we give an outlook on how MADM could be used to probe cell type specific imprinted expression in other tissues in mouse, particularly in extra-embryonic tissues.","lang":"eng"}],"oa_version":"Published Version","external_id":{"isi":["000635575000005"],"pmid":["33600873"]},"type":"journal_article","ec_funded":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","issue":"5","publication_status":"published","date_updated":"2025-04-14T07:43:04Z","fulldoi":"https://doi.org/10.1016/j.neuint.2021.104986","keyword":["Cell Biology","Cellular and Molecular Neuroscience"],"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","pmid":1,"article_number":"104986","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","department":[{"_id":"SiHi"}]},{"_id":"9379","date_created":"2021-05-09T22:01:37Z","file_date_updated":"2021-05-11T15:09:23Z","publisher":"Frontiers Media","publication_identifier":{"eissn":["2296-634X"]},"scopus_import":"1","file":[{"success":1,"checksum":"8c8a03575d2f7583f88dc3b658b0976b","relation":"main_file","creator":"kschuh","file_id":"9386","access_level":"open_access","file_size":4076024,"content_type":"application/pdf","date_updated":"2021-05-11T15:09:23Z","file_name":"2021_Frontiers_Cell_Bolger-Munro.pdf","date_created":"2021-05-11T15:09:23Z"}],"quality_controlled":"1","title":"The Wdr1-LIMK-Cofilin axis controls B cell antigen receptor-induced actin remodeling and signaling at the immune synapse","article_type":"original","author":[{"last_name":"Bolger-Munro","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","full_name":"Bolger-Munro, Madison","first_name":"Madison","orcid":"0000-0002-8176-4824"},{"full_name":"Choi, Kate","last_name":"Choi","first_name":"Kate"},{"full_name":"Cheung, Faith","last_name":"Cheung","first_name":"Faith"},{"last_name":"Liu","full_name":"Liu, Yi Tian","first_name":"Yi Tian"},{"first_name":"May","last_name":"Dang-Lawson","full_name":"Dang-Lawson, May"},{"full_name":"Deretic, Nikola","last_name":"Deretic","first_name":"Nikola"},{"last_name":"Keane","full_name":"Keane, Connor","first_name":"Connor"},{"last_name":"Gold","full_name":"Gold, Michael R.","first_name":"Michael R."}],"publication":"Frontiers in Cell and Developmental Biology","volume":9,"day":"13","acknowledgement":"We thank the UBC Life Sciences Institute Imaging Facility andthe UBC Flow Cytometry Facility.","year":"2021","language":[{"iso":"eng"}],"oa":1,"intvolume":"         9","date_published":"2021-04-13T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"citation":{"ista":"Bolger-Munro M, Choi K, Cheung F, Liu YT, Dang-Lawson M, Deretic N, Keane C, Gold MR. 2021. The Wdr1-LIMK-Cofilin axis controls B cell antigen receptor-induced actin remodeling and signaling at the immune synapse. Frontiers in Cell and Developmental Biology. 9, 649433.","ama":"Bolger-Munro M, Choi K, Cheung F, et al. The Wdr1-LIMK-Cofilin axis controls B cell antigen receptor-induced actin remodeling and signaling at the immune synapse. <i>Frontiers in Cell and Developmental Biology</i>. 2021;9. doi:<a href=\"https://doi.org/10.3389/fcell.2021.649433\">10.3389/fcell.2021.649433</a>","chicago":"Bolger-Munro, Madison, Kate Choi, Faith Cheung, Yi Tian Liu, May Dang-Lawson, Nikola Deretic, Connor Keane, and Michael R. Gold. “The Wdr1-LIMK-Cofilin Axis Controls B Cell Antigen Receptor-Induced Actin Remodeling and Signaling at the Immune Synapse.” <i>Frontiers in Cell and Developmental Biology</i>. Frontiers Media, 2021. <a href=\"https://doi.org/10.3389/fcell.2021.649433\">https://doi.org/10.3389/fcell.2021.649433</a>.","ieee":"M. Bolger-Munro <i>et al.</i>, “The Wdr1-LIMK-Cofilin axis controls B cell antigen receptor-induced actin remodeling and signaling at the immune synapse,” <i>Frontiers in Cell and Developmental Biology</i>, vol. 9. Frontiers Media, 2021.","short":"M. Bolger-Munro, K. Choi, F. Cheung, Y.T. Liu, M. Dang-Lawson, N. Deretic, C. Keane, M.R. Gold, Frontiers in Cell and Developmental Biology 9 (2021).","mla":"Bolger-Munro, Madison, et al. “The Wdr1-LIMK-Cofilin Axis Controls B Cell Antigen Receptor-Induced Actin Remodeling and Signaling at the Immune Synapse.” <i>Frontiers in Cell and Developmental Biology</i>, vol. 9, 649433, Frontiers Media, 2021, doi:<a href=\"https://doi.org/10.3389/fcell.2021.649433\">10.3389/fcell.2021.649433</a>.","apa":"Bolger-Munro, M., Choi, K., Cheung, F., Liu, Y. T., Dang-Lawson, M., Deretic, N., … Gold, M. R. (2021). The Wdr1-LIMK-Cofilin axis controls B cell antigen receptor-induced actin remodeling and signaling at the immune synapse. <i>Frontiers in Cell and Developmental Biology</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fcell.2021.649433\">https://doi.org/10.3389/fcell.2021.649433</a>"},"ddc":["570"],"status":"public","isi":1,"doi":"10.3389/fcell.2021.649433","month":"04","abstract":[{"lang":"eng","text":"When B cells encounter membrane-bound antigens, the formation and coalescence of B cell antigen receptor (BCR) microclusters amplifies BCR signaling. The ability of B cells to probe the surface of antigen-presenting cells (APCs) and respond to APC-bound antigens requires remodeling of the actin cytoskeleton. Initial BCR signaling stimulates actin-related protein (Arp) 2/3 complex-dependent actin polymerization, which drives B cell spreading as well as the centripetal movement and coalescence of BCR microclusters at the B cell-APC synapse. Sustained actin polymerization depends on concomitant actin filament depolymerization, which enables the recycling of actin monomers and Arp2/3 complexes. Cofilin-mediated severing of actin filaments is a rate-limiting step in the morphological changes that occur during immune synapse formation. Hence, regulators of cofilin activity such as WD repeat-containing protein 1 (Wdr1), LIM domain kinase (LIMK), and coactosin-like 1 (Cotl1) may also be essential for actin-dependent processes in B cells. Wdr1 enhances cofilin-mediated actin disassembly. Conversely, Cotl1 competes with cofilin for binding to actin and LIMK phosphorylates cofilin and prevents it from binding to actin filaments. We now show that Wdr1 and LIMK have distinct roles in BCR-induced assembly of the peripheral actin structures that drive B cell spreading, and that cofilin, Wdr1, and LIMK all contribute to the actin-dependent amplification of BCR signaling at the immune synapse. Depleting Cotl1 had no effect on these processes. Thus, the Wdr1-LIMK-cofilin axis is critical for BCR-induced actin remodeling and for B cell responses to APC-bound antigens."}],"oa_version":"Published Version","type":"journal_article","external_id":{"pmid":["33928084"],"isi":["000644419500001"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2023-10-18T08:19:49Z","fulldoi":"https://doi.org/10.3389/fcell.2021.649433","publication_status":"published","keyword":["B cell","actin","immune synapse","cell spreading","cofilin","WDR1 (AIP1)","LIM domain kinase","B cell receptor (BCR)"],"pmid":1,"article_number":"649433","has_accepted_license":"1","article_processing_charge":"No","department":[{"_id":"CaHe"}]},{"acknowledgement":"We are grateful to Paul Knox, Markus Pauly, Malcom O’Neill, and Ignacio Zarra for providing published material; the BOKU-VIBT Imaging Center for access and M. Debreczeny for expertise; J.I. Thaker and Georg Seifert for critical reading.\r\n","day":"26","publication":"International Journal of Molecular Sciences","volume":22,"intvolume":"        22","oa":1,"corr_author":"1","language":[{"iso":"eng"}],"year":"2021","file_date_updated":"2021-09-07T09:04:53Z","_id":"9986","date_created":"2021-09-05T22:01:24Z","author":[{"first_name":"Silvia Melina","full_name":"Velasquez, Silvia Melina","last_name":"Velasquez"},{"full_name":"Guo, Xiaoyuan","last_name":"Guo","first_name":"Xiaoyuan"},{"orcid":"0000-0003-4675-6893","first_name":"Marçal","full_name":"Gallemi, Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","last_name":"Gallemi"},{"full_name":"Aryal, Bibek","last_name":"Aryal","first_name":"Bibek"},{"first_name":"Peter","full_name":"Venhuizen, Peter","last_name":"Venhuizen"},{"first_name":"Elke","last_name":"Barbez","full_name":"Barbez, Elke"},{"last_name":"Dünser","full_name":"Dünser, Kai Alexander","first_name":"Kai Alexander"},{"full_name":"Darino, Martin","last_name":"Darino","first_name":"Martin"},{"first_name":"Aleš","full_name":"Pӗnčík, Aleš","last_name":"Pӗnčík"},{"first_name":"Ondřej","last_name":"Novák","full_name":"Novák, Ondřej"},{"last_name":"Kalyna","full_name":"Kalyna, Maria","first_name":"Maria"},{"last_name":"Mouille","full_name":"Mouille, Gregory","first_name":"Gregory"},{"full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"last_name":"Bhalerao","full_name":"Bhalerao, Rishikesh P.","first_name":"Rishikesh P."},{"first_name":"Jozef","full_name":"Mravec, Jozef","last_name":"Mravec"},{"last_name":"Kleine-Vehn","full_name":"Kleine-Vehn, Jürgen","first_name":"Jürgen"}],"article_type":"original","title":"Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants","quality_controlled":"1","file":[{"content_type":"application/pdf","file_name":"2021_IntJMolecularSciences_Velasquez.pdf","date_updated":"2021-09-07T09:04:53Z","date_created":"2021-09-06T12:50:19Z","file_size":2162247,"checksum":"6b7055cf89f1b7ed8594c3fdf56f000b","file_id":"9988","access_level":"open_access","creator":"cchlebak","relation":"main_file"}],"scopus_import":"1","publication_identifier":{"issn":["1661-6596"],"eissn":["1422-0067"]},"publisher":"MDPI","keyword":["auxin","growth","cell wall","xyloglucans","hypocotyls","gravitropism"],"publication_status":"published","issue":"17","date_updated":"2024-10-09T21:00:50Z","fulldoi":"https://doi.org/10.3390/ijms22179222","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"EvBe"}],"article_processing_charge":"Yes","has_accepted_license":"1","article_number":"9222","pmid":1,"doi":"10.3390/ijms22179222","isi":1,"status":"public","citation":{"mla":"Velasquez, Silvia Melina, et al. “Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 17, 9222, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/ijms22179222\">10.3390/ijms22179222</a>.","apa":"Velasquez, S. M., Guo, X., Gallemi, M., Aryal, B., Venhuizen, P., Barbez, E., … Kleine-Vehn, J. (2021). Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms22179222\">https://doi.org/10.3390/ijms22179222</a>","ieee":"S. M. Velasquez <i>et al.</i>, “Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants,” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 17. MDPI, 2021.","short":"S.M. Velasquez, X. Guo, M. Gallemi, B. Aryal, P. Venhuizen, E. Barbez, K.A. Dünser, M. Darino, A. Pӗnčík, O. Novák, M. Kalyna, G. Mouille, E. Benková, R.P. Bhalerao, J. Mravec, J. Kleine-Vehn, International Journal of Molecular Sciences 22 (2021).","ama":"Velasquez SM, Guo X, Gallemi M, et al. Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. <i>International Journal of Molecular Sciences</i>. 2021;22(17). doi:<a href=\"https://doi.org/10.3390/ijms22179222\">10.3390/ijms22179222</a>","chicago":"Velasquez, Silvia Melina, Xiaoyuan Guo, Marçal Gallemi, Bibek Aryal, Peter Venhuizen, Elke Barbez, Kai Alexander Dünser, et al. “Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal of Molecular Sciences</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/ijms22179222\">https://doi.org/10.3390/ijms22179222</a>.","ista":"Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA, Darino M, Pӗnčík A, Novák O, Kalyna M, Mouille G, Benková E, Bhalerao RP, Mravec J, Kleine-Vehn J. 2021. Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. International Journal of Molecular Sciences. 22(17), 9222."},"ddc":["575"],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"date_published":"2021-08-26T00:00:00Z","external_id":{"isi":["000694347100001"],"pmid":["34502129"]},"type":"journal_article","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Size control is a fundamental question in biology, showing incremental complexity in plants, whose cells possess a rigid cell wall. The phytohormone auxin is a vital growth regulator with central importance for differential growth control. Our results indicate that auxin-reliant growth programs affect the molecular complexity of xyloglucans, the major type of cell wall hemicellulose in eudicots. Auxin-dependent induction and repression of growth coincide with reduced and enhanced molecular complexity of xyloglucans, respectively. In agreement with a proposed function in growth control, genetic interference with xyloglucan side decorations distinctly modulates auxin-dependent differential growth rates. Our work proposes that auxin-dependent growth programs have a spatially defined effect on xyloglucan’s molecular structure, which in turn affects cell wall mechanics and specifies differential, gravitropic hypocotyl growth."}],"month":"08"},{"file_date_updated":"2022-05-13T08:03:37Z","_id":"9999","date_created":"2021-09-12T22:01:23Z","title":"Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism","quality_controlled":"1","author":[{"last_name":"Pulgar","full_name":"Pulgar, Eduardo","first_name":"Eduardo"},{"full_name":"Schwayer, Cornelia","last_name":"Schwayer","id":"3436488C-F248-11E8-B48F-1D18A9856A87","first_name":"Cornelia","orcid":"0000-0001-5130-2226"},{"full_name":"Guerrero, Néstor","last_name":"Guerrero","first_name":"Néstor"},{"first_name":"Loreto","full_name":"López, Loreto","last_name":"López"},{"first_name":"Susana","last_name":"Márquez","full_name":"Márquez, Susana"},{"last_name":"Härtel","full_name":"Härtel, Steffen","first_name":"Steffen"},{"first_name":"Rodrigo","last_name":"Soto","full_name":"Soto, Rodrigo"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl Philipp","first_name":"Carl Philipp"},{"last_name":"Concha","full_name":"Concha, Miguel L.","first_name":"Miguel L."}],"article_type":"original","publication_identifier":{"eissn":["2050-084X"]},"publisher":"eLife Sciences Publications","file":[{"success":1,"checksum":"a3f82b0499cc822ac1eab48a01f3f57e","relation":"main_file","creator":"dernst","file_id":"11371","access_level":"open_access","file_size":9010446,"content_type":"application/pdf","file_name":"2021_eLife_Pulgar.pdf","date_updated":"2022-05-13T08:03:37Z","date_created":"2022-05-13T08:03:37Z"}],"scopus_import":"1","day":"27","volume":10,"publication":"eLife","oa":1,"intvolume":"        10","year":"2021","language":[{"iso":"eng"}],"project":[{"call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573"}],"status":"public","isi":1,"doi":"10.7554/eLife.66483","date_published":"2021-08-27T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"ddc":["570"],"citation":{"apa":"Pulgar, E., Schwayer, C., Guerrero, N., López, L., Márquez, S., Härtel, S., … Concha, M. L. (2021). Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.66483\">https://doi.org/10.7554/eLife.66483</a>","mla":"Pulgar, Eduardo, et al. “Apical Contacts Stemming from Incomplete Delamination Guide Progenitor Cell Allocation through a Dragging Mechanism.” <i>ELife</i>, vol. 10, e66483, eLife Sciences Publications, 2021, doi:<a href=\"https://doi.org/10.7554/eLife.66483\">10.7554/eLife.66483</a>.","ieee":"E. Pulgar <i>et al.</i>, “Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021.","short":"E. Pulgar, C. Schwayer, N. Guerrero, L. López, S. Márquez, S. Härtel, R. Soto, C.P. Heisenberg, M.L. Concha, ELife 10 (2021).","ista":"Pulgar E, Schwayer C, Guerrero N, López L, Márquez S, Härtel S, Soto R, Heisenberg CP, Concha ML. 2021. Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. eLife. 10, e66483.","ama":"Pulgar E, Schwayer C, Guerrero N, et al. Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. <i>eLife</i>. 2021;10. doi:<a href=\"https://doi.org/10.7554/eLife.66483\">10.7554/eLife.66483</a>","chicago":"Pulgar, Eduardo, Cornelia Schwayer, Néstor Guerrero, Loreto López, Susana Márquez, Steffen Härtel, Rodrigo Soto, Carl Philipp Heisenberg, and Miguel L. Concha. “Apical Contacts Stemming from Incomplete Delamination Guide Progenitor Cell Allocation through a Dragging Mechanism.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href=\"https://doi.org/10.7554/eLife.66483\">https://doi.org/10.7554/eLife.66483</a>."},"oa_version":"Published Version","type":"journal_article","external_id":{"isi":["000700428500001"],"pmid":["34448451"]},"month":"08","abstract":[{"text":"The developmental strategies used by progenitor cells to endure a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. Here we uncovered a progenitor cell allocation mechanism that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. Progenitors of the zebrafish laterality organ originate from the surface epithelial enveloping layer by an apical constriction process of cell delamination. During this process, progenitors retain long-term apical contacts that enable the epithelial layer to pull a subset of progenitors along their way towards the vegetal pole. The remaining delaminated progenitors follow apically-attached progenitors’ movement by a co-attraction mechanism, avoiding sequestration by the adjacent endoderm, ensuring their fate and collective allocation at the differentiation site. Thus, we reveal that incomplete delamination serves as a cellular platform for coordinated tissue movements during development. Impact Statement: Incomplete delamination serves as a cellular platform for coordinated tissue movements during development, guiding newly formed progenitor cell groups to the differentiation site.","lang":"eng"}],"keyword":["cell delamination","apical constriction","dragging","mechanical forces","collective 18 locomotion","dorsal forerunner cells","zebrafish"],"ec_funded":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","fulldoi":"https://doi.org/10.7554/eLife.66483","date_updated":"2025-04-14T07:46:58Z","article_processing_charge":"Yes","has_accepted_license":"1","department":[{"_id":"CaHe"}],"pmid":1,"article_number":"e66483"},{"ec_funded":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","date_updated":"2026-10-24T22:30:16Z","fulldoi":"https://doi.org/10.1016/j.ydbio.2020.12.014","keyword":["Developmental Biology","Cell Biology","Molecular Biology"],"pmid":1,"article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","department":[{"_id":"CaHe"}],"date_published":"2021-06-01T00:00:00Z","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"ddc":["570"],"citation":{"apa":"Schauer, A., &#38; Heisenberg, C.-P. J. (2021). Reassembling gastrulation. <i>Developmental Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ydbio.2020.12.014\">https://doi.org/10.1016/j.ydbio.2020.12.014</a>","mla":"Schauer, Alexandra, and Carl-Philipp J. Heisenberg. “Reassembling Gastrulation.” <i>Developmental Biology</i>, vol. 474, Elsevier, 2021, pp. 71–81, doi:<a href=\"https://doi.org/10.1016/j.ydbio.2020.12.014\">10.1016/j.ydbio.2020.12.014</a>.","ista":"Schauer A, Heisenberg C-PJ. 2021. Reassembling gastrulation. Developmental Biology. 474, 71–81.","ama":"Schauer A, Heisenberg C-PJ. Reassembling gastrulation. <i>Developmental Biology</i>. 2021;474:71-81. doi:<a href=\"https://doi.org/10.1016/j.ydbio.2020.12.014\">10.1016/j.ydbio.2020.12.014</a>","chicago":"Schauer, Alexandra, and Carl-Philipp J Heisenberg. “Reassembling Gastrulation.” <i>Developmental Biology</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.ydbio.2020.12.014\">https://doi.org/10.1016/j.ydbio.2020.12.014</a>.","ieee":"A. Schauer and C.-P. J. Heisenberg, “Reassembling gastrulation,” <i>Developmental Biology</i>, vol. 474. Elsevier, pp. 71–81, 2021.","short":"A. Schauer, C.-P.J. Heisenberg, Developmental Biology 474 (2021) 71–81."},"status":"public","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"_id":"26B1E39C-B435-11E9-9278-68D0E5697425","grant_number":"25239","name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues"}],"doi":"10.1016/j.ydbio.2020.12.014","isi":1,"month":"06","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"12891"}]},"abstract":[{"text":"During development, a single cell is transformed into a highly complex organism through progressive cell division, specification and rearrangement. An important prerequisite for the emergence of patterns within the developing organism is to establish asymmetries at various scales, ranging from individual cells to the entire embryo, eventually giving rise to the different body structures. This becomes especially apparent during gastrulation, when the earliest major lineage restriction events lead to the formation of the different germ layers. Traditionally, the unfolding of the developmental program from symmetry breaking to germ layer formation has been studied by dissecting the contributions of different signaling pathways and cellular rearrangements in the in vivo context of intact embryos. Recent efforts, using the intrinsic capacity of embryonic stem cells to self-assemble and generate embryo-like structures de novo, have opened new avenues for understanding the many ways by which an embryo can be built and the influence of extrinsic factors therein. Here, we discuss and compare divergent and conserved strategies leading to germ layer formation in embryos as compared to in vitro systems, their upstream molecular cascades and the role of extrinsic factors in this process.","lang":"eng"}],"oa_version":"Published Version","external_id":{"pmid":["33352181"],"isi":["000639461800008"]},"type":"journal_article","publication":"Developmental Biology","volume":474,"acknowledgement":"We thank Nicoletta Petridou, Diana Pinheiro, Cornelia Schwayer and Stefania Tavano for feedback on the manuscript. Research in the Heisenberg lab is supported by an ERC Advanced Grant (MECSPEC 742573) to C.-P.H. A.S. is a recipient of a DOC Fellowship of the Austrian Academy of Science.","day":"01","year":"2021","language":[{"iso":"eng"}],"oa":1,"intvolume":"       474","file_date_updated":"2021-08-11T10:28:06Z","_id":"8966","page":"71-81","date_created":"2020-12-22T09:53:34Z","publication_identifier":{"issn":["0012-1606"]},"publisher":"Elsevier","file":[{"file_id":"9880","access_level":"open_access","relation":"main_file","creator":"kschuh","success":1,"checksum":"fa2a5731fd16ab171b029f32f031c440","content_type":"application/pdf","date_updated":"2021-08-11T10:28:06Z","file_name":"2021_DevBiology_Schauer.pdf","date_created":"2021-08-11T10:28:06Z","file_size":1440321}],"scopus_import":"1","title":"Reassembling gastrulation","quality_controlled":"1","article_type":"original","author":[{"orcid":"0000-0001-7659-9142","first_name":"Alexandra","last_name":"Schauer","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","full_name":"Schauer, Alexandra"},{"first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}]},{"ddc":["570"],"citation":{"ama":"Hansen AH. Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration. 2021. doi:<a href=\"https://doi.org/10.15479/at:ista:9962\">10.15479/at:ista:9962</a>","ista":"Hansen AH. 2021. Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration. Institute of Science and Technology Austria.","chicago":"Hansen, Andi H. “Cell-Autonomous Gene Function and Non-Cell-Autonomous Effects in Radial Projection Neuron Migration.” Institute of Science and Technology Austria, 2021. <a href=\"https://doi.org/10.15479/at:ista:9962\">https://doi.org/10.15479/at:ista:9962</a>.","short":"A.H. Hansen, Cell-Autonomous Gene Function and Non-Cell-Autonomous Effects in Radial Projection Neuron Migration, Institute of Science and Technology Austria, 2021.","ieee":"A. H. Hansen, “Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration,” Institute of Science and Technology Austria, 2021.","apa":"Hansen, A. H. (2021). <i>Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:9962\">https://doi.org/10.15479/at:ista:9962</a>","mla":"Hansen, Andi H. <i>Cell-Autonomous Gene Function and Non-Cell-Autonomous Effects in Radial Projection Neuron Migration</i>. Institute of Science and Technology Austria, 2021, doi:<a href=\"https://doi.org/10.15479/at:ista:9962\">10.15479/at:ista:9962</a>."},"date_published":"2021-09-02T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"doi":"10.15479/at:ista:9962","project":[{"name":"Molecular mechanisms of radial neuronal migration","grant_number":"24812","_id":"2625A13E-B435-11E9-9278-68D0E5697425"}],"status":"public","abstract":[{"lang":"eng","text":"The brain is one of the largest and most complex organs and it is composed of billions of neurons that communicate together enabling e.g. consciousness. The cerebral cortex is the largest site of neural integration in the central nervous system. Concerted radial migration of newly born cortical projection neurons, from their birthplace to their final position, is a key step in the assembly of the cerebral cortex. The cellular and molecular mechanisms regulating radial neuronal migration in vivo are however still unclear. Recent evidence suggests that distinct signaling cues act cell-autonomously but differentially at certain steps during the overall migration process. Moreover, functional analysis of genetic mosaics (mutant neurons present in wild-type/heterozygote environment) using the MADM (Mosaic Analysis with Double Markers) analyses in comparison to global knockout also indicate a significant degree of non-cell-autonomous and/or community effects in the control of cortical neuron migration. The interactions of cell-intrinsic (cell-autonomous) and cell-extrinsic (non-cell-autonomous) components are largely unknown. In part of this thesis work we established a MADM-based experimental strategy for the quantitative analysis of cell-autonomous gene function versus non-cell-autonomous and/or community effects. The direct comparison of mutant neurons from the genetic mosaic (cell-autonomous) to mutant neurons in the conditional and/or global knockout (cell-autonomous + non-cell-autonomous) allows to quantitatively analyze non-cell-autonomous effects. Such analysis enable the high-resolution analysis of projection neuron migration dynamics in distinct environments with concomitant isolation of genomic and proteomic profiles. Using these experimental paradigms and in combination with computational modeling we show and characterize the nature of non-cell-autonomous effects to coordinate radial neuron migration. Furthermore, this thesis discusses recent developments in neurodevelopment with focus on neuronal polarization and non-cell-autonomous mechanisms in neuronal migration."}],"related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"8569"},{"id":"960","status":"public","relation":"part_of_dissertation"}]},"month":"09","type":"dissertation","oa_version":"Published Version","fulldoi":"https://doi.org/10.15479/at:ista:9962","date_updated":"2026-04-08T07:19:09Z","publication_status":"published","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","keyword":["Neuronal migration","Non-cell-autonomous","Cell-autonomous","Neurodevelopmental disease"],"alternative_title":["ISTA Thesis"],"department":[{"_id":"GradSch"},{"_id":"SiHi"}],"has_accepted_license":"1","article_processing_charge":"No","_id":"9962","page":"182","date_created":"2021-08-29T12:36:50Z","file_date_updated":"2022-09-03T22:30:04Z","file":[{"file_size":10629190,"date_updated":"2022-09-03T22:30:04Z","file_name":"Thesis_Hansen.docx","date_created":"2021-08-30T09:17:39Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","embargo_to":"open_access","creator":"ahansen","relation":"source_file","access_level":"closed","file_id":"9971","checksum":"66b56f5b988b233dc66a4f4b4fb2cdfe"},{"checksum":"204fa40321a1c6289b68c473634c4bf3","relation":"main_file","creator":"ahansen","access_level":"open_access","file_id":"9972","embargo":"2022-09-02","file_size":13457469,"date_created":"2021-08-30T09:29:44Z","date_updated":"2022-09-03T22:30:04Z","file_name":"Thesis_Hansen_PDFA-1a.pdf","content_type":"application/pdf"}],"publisher":"Institute of Science and Technology Austria","publication_identifier":{"issn":["2663-337X"]},"author":[{"id":"38853E16-F248-11E8-B48F-1D18A9856A87","full_name":"Hansen, Andi H","last_name":"Hansen","first_name":"Andi H"}],"title":"Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration","day":"02","degree_awarded":"PhD","language":[{"iso":"eng"}],"OA_place":"publisher","corr_author":"1","year":"2021","supervisor":[{"orcid":"0000-0003-2279-1061","first_name":"Simon","last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","full_name":"Hippenmeyer, Simon"}],"oa":1},{"_id":"8402","date_created":"2020-09-17T10:26:53Z","extern":"1","quality_controlled":"1","title":"The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments","author":[{"first_name":"Heike","full_name":"Rampelt, Heike","last_name":"Rampelt"},{"first_name":"Iva","last_name":"Sucec","full_name":"Sucec, Iva"},{"first_name":"Beate","full_name":"Bersch, Beate","last_name":"Bersch"},{"full_name":"Horten, Patrick","last_name":"Horten","first_name":"Patrick"},{"first_name":"Inge","full_name":"Perschil, Inge","last_name":"Perschil"},{"first_name":"Jean-Claude","full_name":"Martinou, Jean-Claude","last_name":"Martinou"},{"full_name":"van der Laan, Martin","last_name":"van der Laan","first_name":"Martin"},{"full_name":"Wiedemann, Nils","last_name":"Wiedemann","first_name":"Nils"},{"id":"7B541462-FAF6-11E9-A490-E8DFE5697425","full_name":"Schanda, Paul","last_name":"Schanda","first_name":"Paul","orcid":"0000-0002-9350-7606"},{"first_name":"Nikolaus","last_name":"Pfanner","full_name":"Pfanner, Nikolaus"}],"DOAJ_listed":"1","article_type":"original","publisher":"Springer Nature","publication_identifier":{"issn":["1741-7007"]},"day":"06","publication":"BMC Biology","volume":18,"oa":1,"intvolume":"        18","year":"2020","language":[{"iso":"eng"}],"OA_place":"publisher","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1186/s12915-019-0733-6"}],"status":"public","OA_type":"gold","doi":"10.1186/s12915-019-0733-6","date_published":"2020-01-06T00:00:00Z","citation":{"mla":"Rampelt, Heike, et al. “The Mitochondrial Carrier Pathway Transports Non-Canonical Substrates with an Odd Number of Transmembrane Segments.” <i>BMC Biology</i>, vol. 18, 2, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1186/s12915-019-0733-6\">10.1186/s12915-019-0733-6</a>.","apa":"Rampelt, H., Sucec, I., Bersch, B., Horten, P., Perschil, I., Martinou, J.-C., … Pfanner, N. (2020). The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments. <i>BMC Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1186/s12915-019-0733-6\">https://doi.org/10.1186/s12915-019-0733-6</a>","ama":"Rampelt H, Sucec I, Bersch B, et al. The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments. <i>BMC Biology</i>. 2020;18. doi:<a href=\"https://doi.org/10.1186/s12915-019-0733-6\">10.1186/s12915-019-0733-6</a>","ista":"Rampelt H, Sucec I, Bersch B, Horten P, Perschil I, Martinou J-C, van der Laan M, Wiedemann N, Schanda P, Pfanner N. 2020. The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments. BMC Biology. 18, 2.","chicago":"Rampelt, Heike, Iva Sucec, Beate Bersch, Patrick Horten, Inge Perschil, Jean-Claude Martinou, Martin van der Laan, Nils Wiedemann, Paul Schanda, and Nikolaus Pfanner. “The Mitochondrial Carrier Pathway Transports Non-Canonical Substrates with an Odd Number of Transmembrane Segments.” <i>BMC Biology</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1186/s12915-019-0733-6\">https://doi.org/10.1186/s12915-019-0733-6</a>.","short":"H. Rampelt, I. Sucec, B. Bersch, P. Horten, I. Perschil, J.-C. Martinou, M. van der Laan, N. Wiedemann, P. Schanda, N. Pfanner, BMC Biology 18 (2020).","ieee":"H. Rampelt <i>et al.</i>, “The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments,” <i>BMC Biology</i>, vol. 18. Springer Nature, 2020."},"oa_version":"Published Version","type":"journal_article","external_id":{"pmid":["31907035"]},"month":"01","abstract":[{"lang":"eng","text":"Background: The mitochondrial pyruvate carrier (MPC) plays a central role in energy metabolism by transporting pyruvate across the inner mitochondrial membrane. Its heterodimeric composition and homology to SWEET and semiSWEET transporters set the MPC apart from the canonical mitochondrial carrier family (named MCF or SLC25). The import of the canonical carriers is mediated by the carrier translocase of the inner membrane (TIM22) pathway and is dependent on their structure, which features an even number of transmembrane segments and both termini in the intermembrane space. The import pathway of MPC proteins has not been elucidated. The odd number of transmembrane segments and positioning of the N-terminus in the matrix argues against an import via the TIM22 carrier pathway but favors an import via the flexible presequence pathway.\r\nResults: Here, we systematically analyzed the import pathways of Mpc2 and Mpc3 and report that, contrary to an expected import via the flexible presequence pathway, yeast MPC proteins with an odd number of transmembrane segments and matrix-exposed N-terminus are imported by the carrier pathway, using the receptor Tom70, small TIM chaperones, and the TIM22 complex. The TIM9·10 complex chaperones MPC proteins through the mitochondrial intermembrane space using conserved hydrophobic motifs that are also required for the interaction with canonical carrier proteins.\r\nConclusions: The carrier pathway can import paired and non-paired transmembrane helices and translocate N-termini to either side of the mitochondrial inner membrane, revealing an unexpected versatility of the mitochondrial import pathway for non-cleavable inner membrane proteins."}],"keyword":["Biotechnology","Plant Science","General Biochemistry","Genetics and Molecular Biology","Developmental Biology","Cell Biology","Physiology","Ecology","Evolution","Behavior and Systematics","Structural Biology","General Agricultural and Biological Sciences"],"user_id":"0043cee0-e5fc-11ee-9736-f83bc23afbf0","fulldoi":"https://doi.org/10.1186/s12915-019-0733-6","date_updated":"2024-10-15T13:23:11Z","publication_status":"published","article_processing_charge":"No","pmid":1,"article_number":"2"},{"article_number":"107633","pmid":1,"department":[{"_id":"FlSc"}],"has_accepted_license":"1","article_processing_charge":"Yes (via OA deal)","fulldoi":"https://doi.org/10.1016/j.jsb.2020.107633","date_updated":"2026-10-24T22:30:04Z","issue":"3","publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","keyword":["electron microscopy","cryo-EM","EM sample preparation","3D printing","cell culture"],"abstract":[{"text":"Cryo-electron microscopy (cryo-EM) of cellular specimens provides insights into biological processes and structures within a native context. However, a major challenge still lies in the efficient and reproducible preparation of adherent cells for subsequent cryo-EM analysis. This is due to the sensitivity of many cellular specimens to the varying seeding and culturing conditions required for EM experiments, the often limited amount of cellular material and also the fragility of EM grids and their substrate. Here, we present low-cost and reusable 3D printed grid holders, designed to improve specimen preparation when culturing challenging cellular samples directly on grids. The described grid holders increase cell culture reproducibility and throughput, and reduce the resources required for cell culturing. We show that grid holders can be integrated into various cryo-EM workflows, including micro-patterning approaches to control cell seeding on grids, and for generating samples for cryo-focused ion beam milling and cryo-electron tomography experiments. Their adaptable design allows for the generation of specialized grid holders customized to a large variety of applications.","lang":"eng"}],"related_material":{"record":[{"id":"14592","status":"public","relation":"used_in_publication"},{"status":"public","id":"12491","relation":"dissertation_contains"}]},"month":"12","type":"journal_article","external_id":{"pmid":["32987119"],"isi":["000600997800008"]},"oa_version":"Published Version","ddc":["570"],"citation":{"mla":"Fäßler, Florian, et al. “3D Printed Cell Culture Grid Holders for Improved Cellular Specimen Preparation in Cryo-Electron Microscopy.” <i>Journal of Structural Biology</i>, vol. 212, no. 3, 107633, Elsevier, 2020, doi:<a href=\"https://doi.org/10.1016/j.jsb.2020.107633\">10.1016/j.jsb.2020.107633</a>.","apa":"Fäßler, F., Zens, B., Hauschild, R., &#38; Schur, F. K. (2020). 3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy. <i>Journal of Structural Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jsb.2020.107633\">https://doi.org/10.1016/j.jsb.2020.107633</a>","ista":"Fäßler F, Zens B, Hauschild R, Schur FK. 2020. 3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy. Journal of Structural Biology. 212(3), 107633.","chicago":"Fäßler, Florian, Bettina Zens, Robert Hauschild, and Florian KM Schur. “3D Printed Cell Culture Grid Holders for Improved Cellular Specimen Preparation in Cryo-Electron Microscopy.” <i>Journal of Structural Biology</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.jsb.2020.107633\">https://doi.org/10.1016/j.jsb.2020.107633</a>.","ama":"Fäßler F, Zens B, Hauschild R, Schur FK. 3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy. <i>Journal of Structural Biology</i>. 2020;212(3). doi:<a href=\"https://doi.org/10.1016/j.jsb.2020.107633\">10.1016/j.jsb.2020.107633</a>","ieee":"F. Fäßler, B. Zens, R. Hauschild, and F. K. Schur, “3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy,” <i>Journal of Structural Biology</i>, vol. 212, no. 3. Elsevier, 2020.","short":"F. Fäßler, B. Zens, R. Hauschild, F.K. Schur, Journal of Structural Biology 212 (2020)."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"date_published":"2020-12-01T00:00:00Z","doi":"10.1016/j.jsb.2020.107633","isi":1,"status":"public","project":[{"grant_number":"P33367","name":"Structure and isoform diversity of the Arp2/3 complex","_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A"},{"name":"NÖ-Fonds Preis für die Jungforscherin des Jahres am IST Austria","_id":"059B463C-7A3F-11EA-A408-12923DDC885E"}],"language":[{"iso":"eng"}],"corr_author":"1","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"year":"2020","intvolume":"       212","oa":1,"publication":"Journal of Structural Biology","volume":212,"day":"01","acknowledgement":"This work was supported by the Austrian Science Fund (FWF, P33367) to FKMS. BZ acknowledges support by the Niederösterreich Fond. This research was also supported by the Scientific Service Units (SSU) of IST Austria through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), the BioImaging Facility (BIF) and the Electron Microscopy Facility (EMF). We thank Georgi Dimchev (IST Austria) and Sonja Jacob (Vienna Biocenter Core Facilities) for testing our grid holders in different experimental setups and Daniel Gütl and the Kondrashov group (IST Austria) for granting us repeated access to their 3D printers. We also thank Jonna Alanko and the Sixt lab (IST Austria) for providing us HeLa cells, primary BL6 mouse tail fibroblasts, NIH 3T3 fibroblasts and human telomerase immortalised foreskin fibroblasts for our experiments. We are thankful to Ori Avinoam and William Wan for helpful comments on the manuscript and also thank Dorotea Fracchiolla (Art&Science) for illustrating the graphical abstract.","scopus_import":"1","file":[{"file_size":7076870,"date_updated":"2020-12-10T14:01:10Z","file_name":"2020_JourStrucBiology_Faessler.pdf","date_created":"2020-12-10T14:01:10Z","content_type":"application/pdf","checksum":"c48cbf594e84fc2f91966ffaafc0918c","success":1,"relation":"main_file","creator":"dernst","access_level":"open_access","file_id":"8937"}],"publisher":"Elsevier","publication_identifier":{"issn":["1047-8477"]},"author":[{"first_name":"Florian","orcid":"0000-0001-7149-769X","last_name":"Fäßler","id":"404F5528-F248-11E8-B48F-1D18A9856A87","full_name":"Fäßler, Florian"},{"full_name":"Zens, Bettina","last_name":"Zens","id":"45FD126C-F248-11E8-B48F-1D18A9856A87","first_name":"Bettina","orcid":"0000-0002-9561-1239"},{"first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"last_name":"Schur","full_name":"Schur, Florian KM","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","orcid":"0000-0003-4790-8078"}],"article_type":"original","quality_controlled":"1","title":"3D printed cell culture grid holders for improved cellular specimen preparation in cryo-electron microscopy","_id":"8586","date_created":"2020-09-29T13:24:06Z","file_date_updated":"2020-12-10T14:01:10Z"},{"external_id":{"isi":["000534387800005"],"pmid":[" 32094266"]},"type":"journal_article","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Efficient migration on adhesive surfaces involves the protrusion of lamellipodial actin networks and their subsequent stabilization by nascent adhesions. The actin-binding protein lamellipodin (Lpd) is thought to play a critical role in lamellipodium protrusion, by delivering Ena/VASP proteins onto the growing plus ends of actin filaments and by interacting with the WAVE regulatory complex, an activator of the Arp2/3 complex, at the leading edge. Using B16-F1 melanoma cell lines, we demonstrate that genetic ablation of Lpd compromises protrusion efficiency and coincident cell migration without altering essential parameters of lamellipodia, including their maximal rate of forward advancement and actin polymerization. We also confirmed lamellipodia and migration phenotypes with CRISPR/Cas9-mediated Lpd knockout Rat2 fibroblasts, excluding cell type-specific effects. Moreover, computer-aided analysis of cell-edge morphodynamics on B16-F1 cell lamellipodia revealed that loss of Lpd correlates with reduced temporal protrusion maintenance as a prerequisite of nascent adhesion formation. We conclude that Lpd optimizes protrusion and nascent adhesion formation by counteracting frequent, chaotic retraction and membrane ruffling.This article has an associated First Person interview with the first author of the paper. "}],"month":"04","doi":"10.1242/jcs.239020","isi":1,"status":"public","project":[{"call_identifier":"FWF","_id":"2674F658-B435-11E9-9278-68D0E5697425","name":"Protein structure and function in filopodia across scales","grant_number":"M02495"}],"citation":{"ama":"Dimchev GA, Amiri B, Humphries AC, et al. Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation. <i>Journal of Cell Science</i>. 2020;133(7). doi:<a href=\"https://doi.org/10.1242/jcs.239020\">10.1242/jcs.239020</a>","chicago":"Dimchev, Georgi A, Behnam Amiri, Ashley C. Humphries, Matthias Schaks, Vanessa Dimchev, Theresia E. B. Stradal, Jan Faix, et al. “Lamellipodin Tunes Cell Migration by Stabilizing Protrusions and Promoting Adhesion Formation.” <i>Journal of Cell Science</i>. The Company of Biologists, 2020. <a href=\"https://doi.org/10.1242/jcs.239020\">https://doi.org/10.1242/jcs.239020</a>.","ista":"Dimchev GA, Amiri B, Humphries AC, Schaks M, Dimchev V, Stradal TEB, Faix J, Krause M, Way M, Falcke M, Rottner K. 2020. Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation. Journal of Cell Science. 133(7), jcs239020.","ieee":"G. A. Dimchev <i>et al.</i>, “Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation,” <i>Journal of Cell Science</i>, vol. 133, no. 7. The Company of Biologists, 2020.","short":"G.A. Dimchev, B. Amiri, A.C. Humphries, M. Schaks, V. Dimchev, T.E.B. Stradal, J. Faix, M. Krause, M. Way, M. Falcke, K. Rottner, Journal of Cell Science 133 (2020).","apa":"Dimchev, G. A., Amiri, B., Humphries, A. C., Schaks, M., Dimchev, V., Stradal, T. E. B., … Rottner, K. (2020). Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.239020\">https://doi.org/10.1242/jcs.239020</a>","mla":"Dimchev, Georgi A., et al. “Lamellipodin Tunes Cell Migration by Stabilizing Protrusions and Promoting Adhesion Formation.” <i>Journal of Cell Science</i>, vol. 133, no. 7, jcs239020, The Company of Biologists, 2020, doi:<a href=\"https://doi.org/10.1242/jcs.239020\">10.1242/jcs.239020</a>."},"ddc":["570"],"date_published":"2020-04-09T00:00:00Z","department":[{"_id":"FlSc"}],"has_accepted_license":"1","article_processing_charge":"No","article_number":"jcs239020","pmid":1,"keyword":["Cell Biology"],"date_updated":"2025-04-15T07:52:13Z","fulldoi":"https://doi.org/10.1242/jcs.239020","publication_status":"published","issue":"7","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","author":[{"orcid":"0000-0001-8370-6161","first_name":"Georgi A","last_name":"Dimchev","full_name":"Dimchev, Georgi A","id":"38C393BE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Amiri","full_name":"Amiri, Behnam","first_name":"Behnam"},{"last_name":"Humphries","full_name":"Humphries, Ashley C.","first_name":"Ashley C."},{"first_name":"Matthias","full_name":"Schaks, Matthias","last_name":"Schaks"},{"last_name":"Dimchev","full_name":"Dimchev, Vanessa","first_name":"Vanessa"},{"last_name":"Stradal","full_name":"Stradal, Theresia E. B.","first_name":"Theresia E. B."},{"full_name":"Faix, Jan","last_name":"Faix","first_name":"Jan"},{"first_name":"Matthias","last_name":"Krause","full_name":"Krause, Matthias"},{"first_name":"Michael","full_name":"Way, Michael","last_name":"Way"},{"first_name":"Martin","full_name":"Falcke, Martin","last_name":"Falcke"},{"full_name":"Rottner, Klemens","last_name":"Rottner","first_name":"Klemens"}],"article_type":"original","quality_controlled":"1","title":"Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation","scopus_import":"1","file":[{"content_type":"application/pdf","file_name":"2020_JournalCellScience_Dimchev.pdf","date_updated":"2020-10-11T22:30:02Z","date_created":"2020-09-17T14:07:51Z","file_size":13493302,"embargo":"2020-10-10","file_id":"8435","access_level":"open_access","creator":"dernst","relation":"main_file","checksum":"ba917e551acc4ece2884b751434df9ae"}],"publisher":"The Company of Biologists","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"_id":"8434","date_created":"2020-09-17T14:00:33Z","file_date_updated":"2020-10-11T22:30:02Z","intvolume":"       133","oa":1,"language":[{"iso":"eng"}],"year":"2020","day":"09","acknowledgement":"This work was supported in part by Deutsche Forschungsgemeinschaft (DFG)[GRK2223/1, RO2414/5-1 (to K.R.), FA350/11-1 (to M.F.) and FA330/11-1 (to J.F.)],as well as by intramural funding from the Helmholtz Association (to T.E.B.S. andK.R.). G.D. was additionally funded by the Austrian Science Fund (FWF) LiseMeitner Program [M-2495]. A.C.H. and M.W. are supported by the Francis CrickInstitute, which receives its core funding from Cancer Research UK [FC001209], theMedical Research Council [FC001209] and the Wellcome Trust [FC001209]. M.K. issupported by the Biotechnology and Biological Sciences Research Council [BB/F011431/1, BB/J000590/1, BB/N000226/1]. Deposited in PMC for release after 6months.","volume":133,"publication":"Journal of Cell Science"}]