[{"page":"33386-33394","pmid":1,"publisher":"American Chemical Society","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publication":"Journal of the American Chemical Society","type":"journal_article","day":"26","date_updated":"2026-01-19T10:57:53Z","status":"public","date_created":"2026-01-08T07:06:27Z","scopus_import":"1","language":[{"iso":"eng"}],"OA_type":"hybrid","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"Yes (in subscription journal)","abstract":[{"lang":"eng","text":"Systems chemistry has emerged as a useful paradigm to access structures and phenomena typically exhibited by living systems, including complex molecular systems such as self-replicators and foldamers. As we progress further toward the noncovalent synthesis of life-like systems, and eventually life itself, it is necessary to gain control over assembly pathways. Dissipative chemical fueling has enabled access to stable populations of (self-assembled) structures that would normally form only transiently. Here, we report a synthetic dynamic combinatorial library, made from a single structurally simple building block, from which a self-replicator and a foldamer can emerge along two distinct and competing pathways through an inter- or intramolecular assembly process, respectively. A fueled chemical reaction cycle is then set up to generate the foldamer transiently, in the presence of the self-replicator. The partitioning of the building block between the folding and self-replication pathways and the duration of the fueled reaction cycles are controlled by adjusting the amount of the chemical fuel. An out-of-equilibrium steady state involving the two assemblies could also be achieved by using a continuous stirred tank reactor with inflow and outflow of material. This work connects the domains of folding and self-replication in synthetic systems through dissipative out-of-equilibrium chemistry. It demonstrates that foldamers and self-replicators, formed from the same building block, can stably coexist if the system is continuously supplied with energy, while at equilibrium, the Gibbs phase rule prohibits such coexistence."}],"doi":"10.1021/jacs.4c09111","external_id":{"pmid":["39590110"]},"volume":146,"extern":"1","main_file_link":[{"url":"https://doi.org/10.1021/jacs.4c09111","open_access":"1"}],"has_accepted_license":"1","oa":1,"publication_identifier":{"issn":["0002-7863"],"eissn":["1520-5126"]},"title":"Simultaneous formation of a foldamer and a self-replicator by out-of-equilibrium dynamic covalent chemistry","_id":"20962","quality_controlled":"1","intvolume":"       146","article_type":"original","year":"2024","publication_status":"published","license":"https://creativecommons.org/licenses/by/4.0/","oa_version":"Published Version","date_published":"2024-11-26T00:00:00Z","OA_place":"publisher","month":"11","issue":"49","citation":{"ama":"Sood A, Mandal PK, Ottelé J, et al. Simultaneous formation of a foldamer and a self-replicator by out-of-equilibrium dynamic covalent chemistry. <i>Journal of the American Chemical Society</i>. 2024;146(49):33386-33394. doi:<a href=\"https://doi.org/10.1021/jacs.4c09111\">10.1021/jacs.4c09111</a>","mla":"Sood, Ankush, et al. “Simultaneous Formation of a Foldamer and a Self-Replicator by out-of-Equilibrium Dynamic Covalent Chemistry.” <i>Journal of the American Chemical Society</i>, vol. 146, no. 49, American Chemical Society, 2024, pp. 33386–94, doi:<a href=\"https://doi.org/10.1021/jacs.4c09111\">10.1021/jacs.4c09111</a>.","ieee":"A. Sood <i>et al.</i>, “Simultaneous formation of a foldamer and a self-replicator by out-of-equilibrium dynamic covalent chemistry,” <i>Journal of the American Chemical Society</i>, vol. 146, no. 49. American Chemical Society, pp. 33386–33394, 2024.","apa":"Sood, A., Mandal, P. K., Ottelé, J., Wu, J., Eleveld, M., Hatai, J., … Otto, S. (2024). Simultaneous formation of a foldamer and a self-replicator by out-of-equilibrium dynamic covalent chemistry. <i>Journal of the American Chemical Society</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/jacs.4c09111\">https://doi.org/10.1021/jacs.4c09111</a>","ista":"Sood A, Mandal PK, Ottelé J, Wu J, Eleveld M, Hatai J, Pappas CG, Huc I, Otto S. 2024. Simultaneous formation of a foldamer and a self-replicator by out-of-equilibrium dynamic covalent chemistry. Journal of the American Chemical Society. 146(49), 33386–33394.","chicago":"Sood, Ankush, Pradeep K Mandal, Jim Ottelé, Juntian Wu, Marcel Eleveld, Joydev Hatai, Charalampos G. Pappas, Ivan Huc, and Sijbren Otto. “Simultaneous Formation of a Foldamer and a Self-Replicator by out-of-Equilibrium Dynamic Covalent Chemistry.” <i>Journal of the American Chemical Society</i>. American Chemical Society, 2024. <a href=\"https://doi.org/10.1021/jacs.4c09111\">https://doi.org/10.1021/jacs.4c09111</a>.","short":"A. Sood, P.K. Mandal, J. Ottelé, J. Wu, M. Eleveld, J. Hatai, C.G. Pappas, I. Huc, S. Otto, Journal of the American Chemical Society 146 (2024) 33386–33394."},"author":[{"first_name":"Ankush","last_name":"Sood","full_name":"Sood, Ankush"},{"id":"6a3def15-d4b4-11ef-9fa9-a24c1f545ec3","orcid":"0000-0001-5996-956X","full_name":"Mandal, Pradeep K","last_name":"Mandal","first_name":"Pradeep K"},{"first_name":"Jim","last_name":"Ottelé","full_name":"Ottelé, Jim"},{"full_name":"Wu, Juntian","last_name":"Wu","first_name":"Juntian"},{"full_name":"Eleveld, Marcel","first_name":"Marcel","last_name":"Eleveld"},{"first_name":"Joydev","last_name":"Hatai","full_name":"Hatai, Joydev"},{"full_name":"Pappas, Charalampos G.","first_name":"Charalampos G.","last_name":"Pappas"},{"full_name":"Huc, Ivan","first_name":"Ivan","last_name":"Huc"},{"last_name":"Otto","first_name":"Sijbren","full_name":"Otto, Sijbren"}],"PlanS_conform":"1","keyword":["Fibers","Foldamers","Macrocycles","Monomers","Peptides","Proteins"]},{"keyword":["hippocampus","mossy fibers","readily releasable pool","electron microscopy"],"author":[{"orcid":"0000-0003-2344-1039","id":"3F8ABDDA-F248-11E8-B48F-1D18A9856A87","last_name":"Kim","first_name":"Olena","full_name":"Kim, Olena"},{"id":"4305C450-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0005-401X","full_name":"Borges Merjane, Carolina","last_name":"Borges Merjane","first_name":"Carolina"},{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M","last_name":"Jonas","first_name":"Peter M"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"11196"}]},"issue":"Suppl. 1","citation":{"apa":"Kim, O., Borges Merjane, C., &#38; Jonas, P. M. (2019). Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy. In <i>Intrinsic Activity</i> (Vol. 7). Innsbruck, Austria: Austrian Pharmacological Society. <a href=\"https://doi.org/10.25006/ia.7.s1-a3.27\">https://doi.org/10.25006/ia.7.s1-a3.27</a>","ieee":"O. Kim, C. Borges Merjane, and P. M. Jonas, “Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy,” in <i>Intrinsic Activity</i>, Innsbruck, Austria, 2019, vol. 7, no. Suppl. 1.","mla":"Kim, Olena, et al. “Functional Analysis of the Docked Vesicle Pool in Hippocampal Mossy Fiber Terminals by Electron Microscopy.” <i>Intrinsic Activity</i>, vol. 7, no. Suppl. 1, A3.27, Austrian Pharmacological Society, 2019, doi:<a href=\"https://doi.org/10.25006/ia.7.s1-a3.27\">10.25006/ia.7.s1-a3.27</a>.","ama":"Kim O, Borges Merjane C, Jonas PM. Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy. In: <i>Intrinsic Activity</i>. Vol 7. Austrian Pharmacological Society; 2019. doi:<a href=\"https://doi.org/10.25006/ia.7.s1-a3.27\">10.25006/ia.7.s1-a3.27</a>","chicago":"Kim, Olena, Carolina Borges Merjane, and Peter M Jonas. “Functional Analysis of the Docked Vesicle Pool in Hippocampal Mossy Fiber Terminals by Electron Microscopy.” In <i>Intrinsic Activity</i>, Vol. 7. Austrian Pharmacological Society, 2019. <a href=\"https://doi.org/10.25006/ia.7.s1-a3.27\">https://doi.org/10.25006/ia.7.s1-a3.27</a>.","short":"O. Kim, C. Borges Merjane, P.M. Jonas, in:, Intrinsic Activity, Austrian Pharmacological Society, 2019.","ista":"Kim O, Borges Merjane C, Jonas PM. 2019. Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy. Intrinsic Activity. ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological Society vol. 7, A3.27."},"month":"09","publication_status":"published","oa_version":"Published Version","year":"2019","date_published":"2019-09-11T00:00:00Z","corr_author":"1","quality_controlled":"1","intvolume":"         7","publication_identifier":{"issn":["2309-8503"]},"_id":"11222","title":"Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy","oa":1,"department":[{"_id":"PeJo"}],"conference":{"location":"Innsbruck, Austria","start_date":"2019-09-25","end_date":"2019-09-27","name":"ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological Society"},"main_file_link":[{"open_access":"1","url":"https://www.intrinsicactivity.org/2019/7/S1/A3.27/"}],"volume":7,"doi":"10.25006/ia.7.s1-a3.27","project":[{"grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse","call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"},{"name":"Presynaptic calcium channels distribution and impact on coupling at the hippocampal mossy fiber synapse","grant_number":"708497","_id":"25BAF7B2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"Zellkommunikation in Gesundheit und Krankheit","grant_number":"W01205","call_identifier":"FWF","_id":"25C3DBB6-B435-11E9-9278-68D0E5697425"},{"_id":"25C5A090-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"Z00312","name":"Synaptic communication in neuronal microcircuits"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","article_processing_charge":"No","article_number":"A3.27","language":[{"iso":"eng"}],"date_created":"2022-04-20T15:06:05Z","status":"public","day":"11","type":"conference_abstract","date_updated":"2026-04-03T22:30:34Z","publication":"Intrinsic Activity","ec_funded":1,"publisher":"Austrian Pharmacological Society","acknowledgement":"This work was supported by the ERC and EU Horizon 2020 (ERC 692692; MSC-IF 708497) and FWF Z 312-B27 Wittgenstein award; W 1205-B09)."}]