---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20962'
abstract:
- lang: eng
  text: Systems chemistry has emerged as a useful paradigm to access structures and
    phenomena typically exhibited by living systems, including complex molecular systems
    such as self-replicators and foldamers. As we progress further toward the noncovalent
    synthesis of life-like systems, and eventually life itself, it is necessary to
    gain control over assembly pathways. Dissipative chemical fueling has enabled
    access to stable populations of (self-assembled) structures that would normally
    form only transiently. Here, we report a synthetic dynamic combinatorial library,
    made from a single structurally simple building block, from which a self-replicator
    and a foldamer can emerge along two distinct and competing pathways through an
    inter- or intramolecular assembly process, respectively. A fueled chemical reaction
    cycle is then set up to generate the foldamer transiently, in the presence of
    the self-replicator. The partitioning of the building block between the folding
    and self-replication pathways and the duration of the fueled reaction cycles are
    controlled by adjusting the amount of the chemical fuel. An out-of-equilibrium
    steady state involving the two assemblies could also be achieved by using a continuous
    stirred tank reactor with inflow and outflow of material. This work connects the
    domains of folding and self-replication in synthetic systems through dissipative
    out-of-equilibrium chemistry. It demonstrates that foldamers and self-replicators,
    formed from the same building block, can stably coexist if the system is continuously
    supplied with energy, while at equilibrium, the Gibbs phase rule prohibits such
    coexistence.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Ankush
  full_name: Sood, Ankush
  last_name: Sood
- first_name: Pradeep K
  full_name: Mandal, Pradeep K
  id: 6a3def15-d4b4-11ef-9fa9-a24c1f545ec3
  last_name: Mandal
  orcid: 0000-0001-5996-956X
- first_name: Jim
  full_name: Ottelé, Jim
  last_name: Ottelé
- first_name: Juntian
  full_name: Wu, Juntian
  last_name: Wu
- first_name: Marcel
  full_name: Eleveld, Marcel
  last_name: Eleveld
- first_name: Joydev
  full_name: Hatai, Joydev
  last_name: Hatai
- first_name: Charalampos G.
  full_name: Pappas, Charalampos G.
  last_name: Pappas
- first_name: Ivan
  full_name: Huc, Ivan
  last_name: Huc
- first_name: Sijbren
  full_name: Otto, Sijbren
  last_name: Otto
citation:
  ama: Sood A, Mandal PK, Ottelé J, et al. Simultaneous formation of a foldamer and
    a self-replicator by out-of-equilibrium dynamic covalent chemistry. <i>Journal
    of the American Chemical Society</i>. 2024;146(49):33386-33394. doi:<a href="https://doi.org/10.1021/jacs.4c09111">10.1021/jacs.4c09111</a>
  apa: Sood, A., Mandal, P. K., Ottelé, J., Wu, J., Eleveld, M., Hatai, J., … Otto,
    S. (2024). Simultaneous formation of a foldamer and a self-replicator by out-of-equilibrium
    dynamic covalent chemistry. <i>Journal of the American Chemical Society</i>. American
    Chemical Society. <a href="https://doi.org/10.1021/jacs.4c09111">https://doi.org/10.1021/jacs.4c09111</a>
  chicago: Sood, Ankush, Pradeep K Mandal, Jim Ottelé, Juntian Wu, Marcel Eleveld,
    Joydev Hatai, Charalampos G. Pappas, Ivan Huc, and Sijbren Otto. “Simultaneous
    Formation of a Foldamer and a Self-Replicator by out-of-Equilibrium Dynamic Covalent
    Chemistry.” <i>Journal of the American Chemical Society</i>. American Chemical
    Society, 2024. <a href="https://doi.org/10.1021/jacs.4c09111">https://doi.org/10.1021/jacs.4c09111</a>.
  ieee: A. Sood <i>et al.</i>, “Simultaneous formation of a foldamer and a self-replicator
    by out-of-equilibrium dynamic covalent chemistry,” <i>Journal of the American
    Chemical Society</i>, vol. 146, no. 49. American Chemical Society, pp. 33386–33394,
    2024.
  ista: Sood A, Mandal PK, Ottelé J, Wu J, Eleveld M, Hatai J, Pappas CG, Huc I, Otto
    S. 2024. Simultaneous formation of a foldamer and a self-replicator by out-of-equilibrium
    dynamic covalent chemistry. Journal of the American Chemical Society. 146(49),
    33386–33394.
  mla: Sood, Ankush, et al. “Simultaneous Formation of a Foldamer and a Self-Replicator
    by out-of-Equilibrium Dynamic Covalent Chemistry.” <i>Journal of the American
    Chemical Society</i>, vol. 146, no. 49, American Chemical Society, 2024, pp. 33386–94,
    doi:<a href="https://doi.org/10.1021/jacs.4c09111">10.1021/jacs.4c09111</a>.
  short: A. Sood, P.K. Mandal, J. Ottelé, J. Wu, M. Eleveld, J. Hatai, C.G. Pappas,
    I. Huc, S. Otto, Journal of the American Chemical Society 146 (2024) 33386–33394.
date_created: 2026-01-08T07:06:27Z
date_published: 2024-11-26T00:00:00Z
date_updated: 2026-01-19T10:57:53Z
day: '26'
doi: 10.1021/jacs.4c09111
extern: '1'
external_id:
  pmid:
  - '39590110'
fulldoi: https://doi.org/10.1021/jacs.4c09111
has_accepted_license: '1'
intvolume: '       146'
issue: '49'
keyword:
- Fibers
- Foldamers
- Macrocycles
- Monomers
- Peptides
- Proteins
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1021/jacs.4c09111
month: '11'
oa: 1
oa_version: Published Version
page: 33386-33394
pmid: 1
publication: Journal of the American Chemical Society
publication_identifier:
  eissn:
  - 1520-5126
  issn:
  - 0002-7863
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Simultaneous formation of a foldamer and a self-replicator by out-of-equilibrium
  dynamic covalent chemistry
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 146
year: '2024'
...
---
_id: '11222'
acknowledgement: This work was supported by the ERC and EU Horizon 2020 (ERC 692692;
  MSC-IF 708497) and FWF Z 312-B27 Wittgenstein award; W 1205-B09).
article_number: A3.27
article_processing_charge: No
author:
- first_name: Olena
  full_name: Kim, Olena
  id: 3F8ABDDA-F248-11E8-B48F-1D18A9856A87
  last_name: Kim
  orcid: 0000-0003-2344-1039
- first_name: Carolina
  full_name: Borges Merjane, Carolina
  id: 4305C450-F248-11E8-B48F-1D18A9856A87
  last_name: Borges Merjane
  orcid: 0000-0003-0005-401X
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: 'Kim O, Borges Merjane C, Jonas PM. Functional analysis of the docked vesicle
    pool in hippocampal mossy fiber terminals by electron microscopy. In: <i>Intrinsic
    Activity</i>. Vol 7. Austrian Pharmacological Society; 2019. doi:<a href="https://doi.org/10.25006/ia.7.s1-a3.27">10.25006/ia.7.s1-a3.27</a>'
  apa: 'Kim, O., Borges Merjane, C., &#38; Jonas, P. M. (2019). Functional analysis
    of the docked vesicle pool in hippocampal mossy fiber terminals by electron microscopy.
    In <i>Intrinsic Activity</i> (Vol. 7). Innsbruck, Austria: Austrian Pharmacological
    Society. <a href="https://doi.org/10.25006/ia.7.s1-a3.27">https://doi.org/10.25006/ia.7.s1-a3.27</a>'
  chicago: Kim, Olena, Carolina Borges Merjane, and Peter M Jonas. “Functional Analysis
    of the Docked Vesicle Pool in Hippocampal Mossy Fiber Terminals by Electron Microscopy.”
    In <i>Intrinsic Activity</i>, Vol. 7. Austrian Pharmacological Society, 2019.
    <a href="https://doi.org/10.25006/ia.7.s1-a3.27">https://doi.org/10.25006/ia.7.s1-a3.27</a>.
  ieee: O. Kim, C. Borges Merjane, and P. M. Jonas, “Functional analysis of the docked
    vesicle pool in hippocampal mossy fiber terminals by electron microscopy,” in
    <i>Intrinsic Activity</i>, Innsbruck, Austria, 2019, vol. 7, no. Suppl. 1.
  ista: 'Kim O, Borges Merjane C, Jonas PM. 2019. Functional analysis of the docked
    vesicle pool in hippocampal mossy fiber terminals by electron microscopy. Intrinsic
    Activity. ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological
    Society vol. 7, A3.27.'
  mla: Kim, Olena, et al. “Functional Analysis of the Docked Vesicle Pool in Hippocampal
    Mossy Fiber Terminals by Electron Microscopy.” <i>Intrinsic Activity</i>, vol.
    7, no. Suppl. 1, A3.27, Austrian Pharmacological Society, 2019, doi:<a href="https://doi.org/10.25006/ia.7.s1-a3.27">10.25006/ia.7.s1-a3.27</a>.
  short: O. Kim, C. Borges Merjane, P.M. Jonas, in:, Intrinsic Activity, Austrian
    Pharmacological Society, 2019.
conference:
  end_date: 2019-09-27
  location: Innsbruck, Austria
  name: 'ANA: Austrian Neuroscience Association ; APHAR: Austrian Pharmacological
    Society'
  start_date: 2019-09-25
corr_author: '1'
date_created: 2022-04-20T15:06:05Z
date_published: 2019-09-11T00:00:00Z
date_updated: 2026-10-06T22:30:04Z
day: '11'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.25006/ia.7.s1-a3.27
ec_funded: 1
fulldoi: https://doi.org/10.25006/ia.7.s1-a3.27
intvolume: '         7'
issue: Suppl. 1
keyword:
- hippocampus
- mossy fibers
- readily releasable pool
- electron microscopy
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.intrinsicactivity.org/2019/7/S1/A3.27/
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '692692'
  name: Biophysics and circuit function of a giant cortical glutamatergic synapse
- _id: 25BAF7B2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '708497'
  name: Presynaptic calcium channels distribution and impact on coupling at the hippocampal
    mossy fiber synapse
- _id: 25C3DBB6-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: W01205
  name: Zellkommunikation in Gesundheit und Krankheit
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Z00312
  name: Synaptic communication in neuronal microcircuits
publication: Intrinsic Activity
publication_identifier:
  issn:
  - 2309-8503
publication_status: published
publisher: Austrian Pharmacological Society
quality_controlled: '1'
related_material:
  record:
  - id: '11196'
    relation: dissertation_contains
    status: public
status: public
title: Functional analysis of the docked vesicle pool in hippocampal mossy fiber terminals
  by electron microscopy
type: conference_abstract
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 7
year: '2019'
...