---
OA_place: publisher
OA_type: free access
_id: '3931'
abstract:
- lang: eng
  text: Hyaluronan is an unsulfated glycosaminoglycan (GAG) that is ubiquitously expressed
    in the extracellular matrix (ECM) of all vertebrates, where hyaluronan rich matrices
    constitute a particular permissive environment for the development of complex
    biological structures and also for tumor progression. Because of its conserved
    structure and ubiquitous expression, antibodies for its histochemical detection
    cannot be produced. We have engineered a fusion protein, neurocan-GFP, and expressed
    it as a secreted molecule in mammalian cells. Neurocan-GFP fusion protein specifically
    binds to hyaluronan and directly visualizes hyaluronan on tissue sections, revealing
    a very detailed picture of hyaluronan distribution. The fluorescent fusion protein
    can be used in combination with antibodies and nuclear markers for double or triple
    staining. In addition, it is suitable to visualize hyaluronan on living cells
    by time-lapse video microscopy. The successful production and application of the
    neurocan-GFP fusion protein opens up new perspectives for using GFP fusion proteins
    as detection tools in histological and cytological studies complementing conventional
    antibody and biotin/avidin techniques.
article_processing_charge: No
article_type: original
author:
- first_name: Hui
  full_name: Zhang, Hui
  last_name: Zhang
- first_name: Stephan
  full_name: Baader, Stephan
  last_name: Baader
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Joachim
  full_name: Kappler, Joachim
  last_name: Kappler
- first_name: Uwe
  full_name: Rauch, Uwe
  last_name: Rauch
citation:
  ama: 'Zhang H, Baader S, Sixt MK, Kappler J, Rauch U. Neurocan-GFP fusion protein:
    a new approach to detect hyaluronan on tissue sections and living cells. <i>Journal
    of Histochemistry and Cytochemistry</i>. 2004;52(7):915-922. doi:<a href="https://doi.org/10.1369/jhc.3A6221.2004">10.1369/jhc.3A6221.2004</a>'
  apa: 'Zhang, H., Baader, S., Sixt, M. K., Kappler, J., &#38; Rauch, U. (2004). Neurocan-GFP
    fusion protein: a new approach to detect hyaluronan on tissue sections and living
    cells. <i>Journal of Histochemistry and Cytochemistry</i>. Histochemical Society.
    <a href="https://doi.org/10.1369/jhc.3A6221.2004">https://doi.org/10.1369/jhc.3A6221.2004</a>'
  chicago: 'Zhang, Hui, Stephan Baader, Michael K Sixt, Joachim Kappler, and Uwe Rauch.
    “Neurocan-GFP Fusion Protein: A New Approach to Detect Hyaluronan on Tissue Sections
    and Living Cells.” <i>Journal of Histochemistry and Cytochemistry</i>. Histochemical
    Society, 2004. <a href="https://doi.org/10.1369/jhc.3A6221.2004">https://doi.org/10.1369/jhc.3A6221.2004</a>.'
  ieee: 'H. Zhang, S. Baader, M. K. Sixt, J. Kappler, and U. Rauch, “Neurocan-GFP
    fusion protein: a new approach to detect hyaluronan on tissue sections and living
    cells,” <i>Journal of Histochemistry and Cytochemistry</i>, vol. 52, no. 7. Histochemical
    Society, pp. 915–922, 2004.'
  ista: 'Zhang H, Baader S, Sixt MK, Kappler J, Rauch U. 2004. Neurocan-GFP fusion
    protein: a new approach to detect hyaluronan on tissue sections and living cells.
    Journal of Histochemistry and Cytochemistry. 52(7), 915–922.'
  mla: 'Zhang, Hui, et al. “Neurocan-GFP Fusion Protein: A New Approach to Detect
    Hyaluronan on Tissue Sections and Living Cells.” <i>Journal of Histochemistry
    and Cytochemistry</i>, vol. 52, no. 7, Histochemical Society, 2004, pp. 915–22,
    doi:<a href="https://doi.org/10.1369/jhc.3A6221.2004">10.1369/jhc.3A6221.2004</a>.'
  short: H. Zhang, S. Baader, M.K. Sixt, J. Kappler, U. Rauch, Journal of Histochemistry
    and Cytochemistry 52 (2004) 915–922.
date_created: 2018-12-11T12:05:57Z
date_published: 2004-07-01T00:00:00Z
date_updated: 2026-09-01T11:22:42Z
day: '01'
doi: 10.1369/jhc.3A6221.2004
extern: '1'
external_id:
  pmid:
  - '15208358'
fulldoi: https://doi.org/10.1369/jhc.3A6221.2004
intvolume: '        52'
issue: '7'
keyword:
- hyaluronan
- affinity histochemistry
- neurocan
- GFP
- fusion protein
- time-lapse video microscopy
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1369/jhc.3A6221.2004
month: '07'
oa: 1
oa_version: Published Version
page: 915 - 922
pmid: 1
publication: Journal of Histochemistry and Cytochemistry
publication_identifier:
  eissn:
  - 1551-5044
  issn:
  - 0022-1554
publication_status: published
publisher: Histochemical Society
publist_id: '2196'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Neurocan-GFP fusion protein: a new approach to detect hyaluronan on tissue
  sections and living cells'
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 52
year: '2004'
...