[{"isi":1,"citation":{"ieee":"L. Hayward and G. Sella, “Polygenic adaptation after a sudden change in environment,” <i>eLife</i>, vol. 11. eLife Sciences Publications, 2022.","mla":"Hayward, Laura, and Guy Sella. “Polygenic Adaptation after a Sudden Change in Environment.” <i>ELife</i>, vol. 11, 66697, eLife Sciences Publications, 2022, doi:<a href=\"https://doi.org/10.7554/elife.66697\">10.7554/elife.66697</a>.","ista":"Hayward L, Sella G. 2022. Polygenic adaptation after a sudden change in environment. eLife. 11, 66697.","chicago":"Hayward, Laura, and Guy Sella. “Polygenic Adaptation after a Sudden Change in Environment.” <i>ELife</i>. eLife Sciences Publications, 2022. <a href=\"https://doi.org/10.7554/elife.66697\">https://doi.org/10.7554/elife.66697</a>.","ama":"Hayward L, Sella G. Polygenic adaptation after a sudden change in environment. <i>eLife</i>. 2022;11. doi:<a href=\"https://doi.org/10.7554/elife.66697\">10.7554/elife.66697</a>","short":"L. Hayward, G. Sella, ELife 11 (2022).","apa":"Hayward, L., &#38; Sella, G. (2022). Polygenic adaptation after a sudden change in environment. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.66697\">https://doi.org/10.7554/elife.66697</a>"},"file":[{"creator":"dernst","checksum":"28de155b231ac1c8d4501c98b2fb359a","success":1,"file_name":"2022_eLife_Hayward.pdf","file_size":18935612,"relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_id":"12363","date_updated":"2023-01-24T12:21:32Z","date_created":"2023-01-24T12:21:32Z"}],"publication_status":"published","scopus_import":"1","author":[{"full_name":"Hayward, Laura","last_name":"Hayward","id":"fc885ee5-24bf-11eb-ad7b-bcc5104c0c1b","first_name":"Laura"},{"full_name":"Sella, Guy","last_name":"Sella","first_name":"Guy"}],"day":"26","publisher":"eLife Sciences Publications","volume":11,"doi":"10.7554/elife.66697","quality_controlled":"1","article_type":"original","month":"09","oa_version":"Published Version","status":"public","article_number":"66697","keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","General Medicine","General Neuroscience"],"ddc":["570"],"acknowledgement":"We thank Guy Amster, Jeremy Berg, Nick Barton, Yuval Simons and Molly Przeworski for many helpful discussions, and Jeremy Berg, Graham Coop, Joachim Hermisson, Guillaume Martin, Will Milligan, Peter Ralph, Yuval Simons, Leo Speidel and Molly Przeworski for comments on the manuscript.\r\nNational Institutes of Health GM115889 Laura Katharine Hayward Guy Sella \r\nNational Institutes of Health GM121372 Laura Katharine Hayward","publication_identifier":{"eissn":["2050-084X"]},"date_updated":"2024-10-09T21:03:38Z","year":"2022","date_created":"2023-01-12T12:09:00Z","publication":"eLife","abstract":[{"text":"Polygenic adaptation is thought to be ubiquitous, yet remains poorly understood. Here, we model this process analytically, in the plausible setting of a highly polygenic, quantitative trait that experiences a sudden shift in the fitness optimum. We show how the mean phenotype changes over time, depending on the effect sizes of loci that contribute to variance in the trait, and characterize the allele dynamics at these loci. Notably, we describe the two phases of the allele dynamics: The first is a rapid phase, in which directional selection introduces small frequency differences between alleles whose effects are aligned with or opposed to the shift, ultimately leading to small differences in their probability of fixation during a second, longer phase, governed by stabilizing selection. As we discuss, key results should hold in more general settings and have important implications for efforts to identify the genetic basis of adaptation in humans and other species.","lang":"eng"}],"external_id":{"isi":["000890735600001"]},"department":[{"_id":"NiBa"}],"oa":1,"corr_author":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","language":[{"iso":"eng"}],"file_date_updated":"2023-01-24T12:21:32Z","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"article_processing_charge":"No","title":"Polygenic adaptation after a sudden change in environment","date_published":"2022-09-26T00:00:00Z","intvolume":"        11","_id":"12157","type":"journal_article","has_accepted_license":"1"},{"file":[{"content_type":"application/pdf","date_updated":"2023-01-27T07:19:11Z","file_id":"12411","date_created":"2023-01-27T07:19:11Z","file_name":"2022_NatureCommunications_Prehal.pdf","file_size":4216931,"access_level":"open_access","relation":"main_file","checksum":"5034336dbf0f860030ef745c08df9e0e","success":1,"creator":"dernst"}],"isi":1,"citation":{"short":"C. Prehal, J.-M. von Mentlen, S. Drvarič Talian, A. Vizintin, R. Dominko, H. Amenitsch, L. Porcar, S.A. Freunberger, V. Wood, Nature Communications 13 (2022).","apa":"Prehal, C., von Mentlen, J.-M., Drvarič Talian, S., Vizintin, A., Dominko, R., Amenitsch, H., … Wood, V. (2022). On the nanoscale structural evolution of solid discharge products in lithium-sulfur batteries using operando scattering. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-33931-4\">https://doi.org/10.1038/s41467-022-33931-4</a>","ama":"Prehal C, von Mentlen J-M, Drvarič Talian S, et al. On the nanoscale structural evolution of solid discharge products in lithium-sulfur batteries using operando scattering. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-33931-4\">10.1038/s41467-022-33931-4</a>","chicago":"Prehal, Christian, Jean-Marc von Mentlen, Sara Drvarič Talian, Alen Vizintin, Robert Dominko, Heinz Amenitsch, Lionel Porcar, Stefan Alexander Freunberger, and Vanessa Wood. “On the Nanoscale Structural Evolution of Solid Discharge Products in Lithium-Sulfur Batteries Using Operando Scattering.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-33931-4\">https://doi.org/10.1038/s41467-022-33931-4</a>.","ista":"Prehal C, von Mentlen J-M, Drvarič Talian S, Vizintin A, Dominko R, Amenitsch H, Porcar L, Freunberger SA, Wood V. 2022. On the nanoscale structural evolution of solid discharge products in lithium-sulfur batteries using operando scattering. Nature Communications. 13, 6326.","mla":"Prehal, Christian, et al. “On the Nanoscale Structural Evolution of Solid Discharge Products in Lithium-Sulfur Batteries Using Operando Scattering.” <i>Nature Communications</i>, vol. 13, 6326, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-33931-4\">10.1038/s41467-022-33931-4</a>.","ieee":"C. Prehal <i>et al.</i>, “On the nanoscale structural evolution of solid discharge products in lithium-sulfur batteries using operando scattering,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022."},"publication_status":"published","scopus_import":"1","author":[{"first_name":"Christian","full_name":"Prehal, Christian","last_name":"Prehal"},{"last_name":"von Mentlen","full_name":"von Mentlen, Jean-Marc","first_name":"Jean-Marc"},{"last_name":"Drvarič Talian","full_name":"Drvarič Talian, Sara","first_name":"Sara"},{"first_name":"Alen","last_name":"Vizintin","full_name":"Vizintin, Alen"},{"first_name":"Robert","last_name":"Dominko","full_name":"Dominko, Robert"},{"first_name":"Heinz","last_name":"Amenitsch","full_name":"Amenitsch, Heinz"},{"first_name":"Lionel","full_name":"Porcar, Lionel","last_name":"Porcar"},{"orcid":"0000-0003-2902-5319","first_name":"Stefan Alexander","id":"A8CA28E6-CE23-11E9-AD2D-EC27E6697425","full_name":"Freunberger, Stefan Alexander","last_name":"Freunberger"},{"first_name":"Vanessa","full_name":"Wood, Vanessa","last_name":"Wood"}],"day":"24","publisher":"Springer Nature","volume":13,"quality_controlled":"1","doi":"10.1038/s41467-022-33931-4","article_type":"original","month":"10","oa_version":"Published Version","status":"public","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"article_number":"6326","ddc":["540"],"acknowledgement":"This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant NanoEvolution, grant agreement No 894042. The authors acknowledge the CERIC-ERIC Consortium for the access to the Austrian SAXS beamline and TU Graz for support through the Lead Project LP-03.\r\nLikewise, the use of SOMAPP Lab, a core facility supported by the Austrian Federal Ministry of Education, Science and Research, the Graz University of Technology, the University of Graz, and Anton Paar GmbH is acknowledged. In addition, the authors acknowledge access to the D-22SANS beamline at the ILL neutron source. Electron microscopy measurements were performed at the Scientific Scenter for Optical and Electron Microscopy (ScopeM) of the Swiss Federal Institute of Technology. C.P. and J.M.M. thank A. Senol for her support with the SANS\r\nbeamtime preparation. S.D.T, A.V. and R.D. acknowledge the financial support by the Slovenian Research Agency (ARRS) research core funding P2-0393 and P2-0423. Furthermore, A.V. acknowledge the funding from the Slovenian Research Agency, research project Z2−1863.\r\nS.A.F. is indebted to IST Austria for support. ","publication_identifier":{"issn":["2041-1723"]},"year":"2022","date_updated":"2024-10-09T21:03:47Z","date_created":"2023-01-16T09:45:09Z","publication":"Nature Communications","abstract":[{"text":"The inadequate understanding of the mechanisms that reversibly convert molecular sulfur (S) into lithium sulfide (Li<jats:sub>2</jats:sub>S) via soluble polysulfides (PSs) formation impedes the development of high-performance lithium-sulfur (Li-S) batteries with non-aqueous electrolyte solutions. Here, we use operando small and wide angle X-ray scattering and operando small angle neutron scattering (SANS) measurements to track the nucleation, growth and dissolution of solid deposits from atomic to sub-micron scales during real-time Li-S cell operation. In particular, stochastic modelling based on the SANS data allows quantifying the nanoscale phase evolution during battery cycling. We show that next to nano-crystalline Li<jats:sub>2</jats:sub>S the deposit comprises solid short-chain PSs particles. The analysis of the experimental data suggests that initially, Li<jats:sub>2</jats:sub>S<jats:sub>2</jats:sub> precipitates from the solution and then is partially converted via solid-state electroreduction to Li<jats:sub>2</jats:sub>S. We further demonstrate that mass transport, rather than electron transport through a thin passivating film, limits the discharge capacity and rate performance in Li-S cells.","lang":"eng"}],"external_id":{"isi":["000871563700006"],"pmid":["36280671"]},"department":[{"_id":"StFr"}],"oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","corr_author":"1","file_date_updated":"2023-01-27T07:19:11Z","language":[{"iso":"eng"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"title":"On the nanoscale structural evolution of solid discharge products in lithium-sulfur batteries using operando scattering","article_processing_charge":"No","date_published":"2022-10-24T00:00:00Z","pmid":1,"intvolume":"        13","type":"journal_article","_id":"12208","has_accepted_license":"1"},{"ec_funded":1,"scopus_import":"1","isi":1,"citation":{"ieee":"S. Randriamanantsoa <i>et al.</i>, “Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.","ista":"Randriamanantsoa S, Papargyriou A, Maurer HC, Peschke K, Schuster M, Zecchin G, Steiger K, Öllinger R, Saur D, Scheel C, Rad R, Hannezo EB, Reichert M, Bausch AR. 2022. Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. Nature Communications. 13, 5219.","chicago":"Randriamanantsoa, S., A. Papargyriou, H. C. Maurer, K. Peschke, M. Schuster, G. Zecchin, K. Steiger, et al. “Spatiotemporal Dynamics of Self-Organized Branching in Pancreas-Derived Organoids.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-32806-y\">https://doi.org/10.1038/s41467-022-32806-y</a>.","mla":"Randriamanantsoa, S., et al. “Spatiotemporal Dynamics of Self-Organized Branching in Pancreas-Derived Organoids.” <i>Nature Communications</i>, vol. 13, 5219, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-32806-y\">10.1038/s41467-022-32806-y</a>.","ama":"Randriamanantsoa S, Papargyriou A, Maurer HC, et al. Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-32806-y\">10.1038/s41467-022-32806-y</a>","apa":"Randriamanantsoa, S., Papargyriou, A., Maurer, H. C., Peschke, K., Schuster, M., Zecchin, G., … Bausch, A. R. (2022). Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-32806-y\">https://doi.org/10.1038/s41467-022-32806-y</a>","short":"S. Randriamanantsoa, A. Papargyriou, H.C. Maurer, K. Peschke, M. Schuster, G. Zecchin, K. Steiger, R. Öllinger, D. Saur, C. Scheel, R. Rad, E.B. Hannezo, M. Reichert, A.R. Bausch, Nature Communications 13 (2022)."},"file":[{"relation":"main_file","file_size":22645149,"access_level":"open_access","file_name":"2022_NatureCommunications_Randriamanantsoa.pdf","date_created":"2023-01-27T08:14:48Z","file_id":"12416","date_updated":"2023-01-27T08:14:48Z","content_type":"application/pdf","creator":"dernst","success":1,"checksum":"295261b5172274fd5b8f85a6a6058828"}],"publication_status":"published","project":[{"name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}],"publisher":"Springer Nature","volume":13,"author":[{"full_name":"Randriamanantsoa, S.","last_name":"Randriamanantsoa","first_name":"S."},{"first_name":"A.","full_name":"Papargyriou, A.","last_name":"Papargyriou"},{"last_name":"Maurer","full_name":"Maurer, H. C.","first_name":"H. C."},{"full_name":"Peschke, K.","last_name":"Peschke","first_name":"K."},{"first_name":"M.","last_name":"Schuster","full_name":"Schuster, M."},{"first_name":"G.","full_name":"Zecchin, G.","last_name":"Zecchin"},{"first_name":"K.","full_name":"Steiger, K.","last_name":"Steiger"},{"first_name":"R.","last_name":"Öllinger","full_name":"Öllinger, R."},{"last_name":"Saur","full_name":"Saur, D.","first_name":"D."},{"first_name":"C.","last_name":"Scheel","full_name":"Scheel, C."},{"last_name":"Rad","full_name":"Rad, R.","first_name":"R."},{"full_name":"Hannezo, Edouard B","last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"},{"full_name":"Reichert, M.","last_name":"Reichert","first_name":"M."},{"first_name":"A. R.","last_name":"Bausch","full_name":"Bausch, A. R."}],"related_material":{"record":[{"relation":"research_data","status":"public","id":"13068"}]},"day":"05","doi":"10.1038/s41467-022-32806-y","quality_controlled":"1","status":"public","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"article_number":"5219","ddc":["570"],"article_type":"original","month":"09","oa_version":"Published Version","abstract":[{"text":"The development dynamics and self-organization of glandular branched epithelia is of utmost importance for our understanding of diverse processes ranging from normal tissue growth to the growth of cancerous tissues. Using single primary murine pancreatic ductal adenocarcinoma (PDAC) cells embedded in a collagen matrix and adapted media supplementation, we generate organoids that self-organize into highly branched structures displaying a seamless lumen connecting terminal end buds, replicating in vivo PDAC architecture. We identify distinct morphogenesis phases, each characterized by a unique pattern of cell invasion, matrix deformation, protein expression, and respective molecular dependencies. We propose a minimal theoretical model of a branching and proliferating tissue, capturing the dynamics of the first phases. Observing the interaction of morphogenesis, mechanical environment and gene expression in vitro sets a benchmark for the understanding of self-organization processes governing complex organoid structure formation processes and branching morphogenesis.","lang":"eng"}],"external_id":{"pmid":["36064947"],"isi":["000850348400025"]},"acknowledgement":"A.R.B. acknowledges the financial support of the European Research Council (ERC) through the funding of the grant Principles of Integrin Mechanics and Adhesion (PoINT) and the German Research Foundation (DFG, SFB 1032, project ID 201269156). E.H. was supported by the European Union (European Research Council Starting Grant 851288). D.S., M.R., and R.R. acknowledge the support by the German Research Foundation (DFG, SFB1321 Modeling and Targeting Pancreatic Cancer, Project S01, project ID 329628492). C.S. and M.R. acknowledge the support by the German Research Foundation (DFG, SFB1321 Modeling and Targeting Pancreatic Cancer, Project 12, project ID 329628492). M.R. was supported by the German Research Foundation (DFG RE 3723/4-1). A.P. and M.R. were supported by the German Cancer Aid (Max-Eder Program 111273 and 70114328).\r\nOpen Access funding enabled and organized by Projekt DEAL.","publication_identifier":{"issn":["2041-1723"]},"date_updated":"2025-06-11T13:53:55Z","year":"2022","publication":"Nature Communications","date_created":"2023-01-16T09:46:53Z","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"EdHa"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"title":"Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids","article_processing_charge":"No","date_published":"2022-09-05T00:00:00Z","pmid":1,"file_date_updated":"2023-01-27T08:14:48Z","language":[{"iso":"eng"}],"_id":"12217","type":"journal_article","has_accepted_license":"1","intvolume":"        13"},{"citation":{"ieee":"M. W. Muhia <i>et al.</i>, “Muskelin regulates actin-dependent synaptic changes and intrinsic brain activity relevant to behavioral and cognitive processes,” <i>Communications Biology</i>, vol. 5. Springer Nature, 2022.","ama":"Muhia MW, YuanXiang P, Sedlacik J, et al. Muskelin regulates actin-dependent synaptic changes and intrinsic brain activity relevant to behavioral and cognitive processes. <i>Communications Biology</i>. 2022;5. doi:<a href=\"https://doi.org/10.1038/s42003-022-03446-1\">10.1038/s42003-022-03446-1</a>","chicago":"Muhia, Mary W, PingAn YuanXiang, Jan Sedlacik, Jürgen R. Schwarz, Frank F. Heisler, Kira V. Gromova, Edda Thies, et al. “Muskelin Regulates Actin-Dependent Synaptic Changes and Intrinsic Brain Activity Relevant to Behavioral and Cognitive Processes.” <i>Communications Biology</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s42003-022-03446-1\">https://doi.org/10.1038/s42003-022-03446-1</a>.","mla":"Muhia, Mary W., et al. “Muskelin Regulates Actin-Dependent Synaptic Changes and Intrinsic Brain Activity Relevant to Behavioral and Cognitive Processes.” <i>Communications Biology</i>, vol. 5, 589, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s42003-022-03446-1\">10.1038/s42003-022-03446-1</a>.","ista":"Muhia MW, YuanXiang P, Sedlacik J, Schwarz JR, Heisler FF, Gromova KV, Thies E, Breiden P, Pechmann Y, Kreutz MR, Kneussel M. 2022. Muskelin regulates actin-dependent synaptic changes and intrinsic brain activity relevant to behavioral and cognitive processes. Communications Biology. 5, 589.","short":"M.W. Muhia, P. YuanXiang, J. Sedlacik, J.R. Schwarz, F.F. Heisler, K.V. Gromova, E. Thies, P. Breiden, Y. Pechmann, M.R. Kreutz, M. Kneussel, Communications Biology 5 (2022).","apa":"Muhia, M. W., YuanXiang, P., Sedlacik, J., Schwarz, J. R., Heisler, F. F., Gromova, K. V., … Kneussel, M. (2022). Muskelin regulates actin-dependent synaptic changes and intrinsic brain activity relevant to behavioral and cognitive processes. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-022-03446-1\">https://doi.org/10.1038/s42003-022-03446-1</a>"},"isi":1,"file":[{"creator":"dernst","checksum":"bd95be1e77090208b79bc45ea8785d0b","success":1,"file_name":"2022_CommBiology_Muhia.pdf","access_level":"open_access","file_size":3968356,"relation":"main_file","content_type":"application/pdf","date_updated":"2023-01-27T08:23:46Z","file_id":"12417","date_created":"2023-01-27T08:23:46Z"}],"publication_status":"published","scopus_import":"1","author":[{"first_name":"Mary W","id":"ab7ed20f-09f7-11eb-909c-d5d0b443ee9d","full_name":"Muhia, Mary W","last_name":"Muhia"},{"last_name":"YuanXiang","full_name":"YuanXiang, PingAn","first_name":"PingAn"},{"full_name":"Sedlacik, Jan","last_name":"Sedlacik","first_name":"Jan"},{"last_name":"Schwarz","full_name":"Schwarz, Jürgen R.","first_name":"Jürgen R."},{"last_name":"Heisler","full_name":"Heisler, Frank F.","first_name":"Frank F."},{"first_name":"Kira V.","full_name":"Gromova, Kira V.","last_name":"Gromova"},{"full_name":"Thies, Edda","last_name":"Thies","first_name":"Edda"},{"first_name":"Petra","full_name":"Breiden, Petra","last_name":"Breiden"},{"last_name":"Pechmann","full_name":"Pechmann, Yvonne","first_name":"Yvonne"},{"first_name":"Michael R.","last_name":"Kreutz","full_name":"Kreutz, Michael R."},{"full_name":"Kneussel, Matthias","last_name":"Kneussel","first_name":"Matthias"}],"day":"15","publisher":"Springer Nature","volume":5,"quality_controlled":"1","doi":"10.1038/s42003-022-03446-1","article_type":"original","month":"06","oa_version":"Published Version","status":"public","article_number":"589","keyword":["General Agricultural and Biological Sciences","General Biochemistry","Genetics and Molecular Biology","Medicine (miscellaneous)"],"ddc":["570"],"acknowledgement":"The authors are grateful to the UKE Animal Facilities (Hamburg) for animal husbandry and Dr. Bastian Tiemann for his veterinary expertise and supervision of animal care. We thank Dr. Franco Lombino for critically reading the manuscript and for helpful discussion. This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG) (FOR2419-KN556/11-1, FOR2419-KN556/11-2, KN556/12-1) and the Landesforschungsförderung Hamburg (LFF-FV76) to M.K.\r\nOpen Access funding enabled and organized by Projekt DEAL.","publication_identifier":{"issn":["2399-3642"]},"date_updated":"2024-10-09T21:03:48Z","year":"2022","date_created":"2023-01-16T09:48:19Z","publication":"Communications Biology","abstract":[{"text":"Muskelin (Mkln1) is implicated in neuronal function, regulating plasma membrane receptor trafficking. However, its influence on intrinsic brain activity and corresponding behavioral processes remains unclear. Here we show that murine <jats:italic>Mkln1</jats:italic> knockout causes non-habituating locomotor activity, increased exploratory drive, and decreased locomotor response to amphetamine. Muskelin deficiency impairs social novelty detection while promoting the retention of spatial reference memory and fear extinction recall. This is strongly mirrored in either weaker or stronger resting-state functional connectivity between critical circuits mediating locomotor exploration and cognition. We show that <jats:italic>Mkln1</jats:italic> deletion alters dendrite branching and spine structure, coinciding with enhanced AMPAR-mediated synaptic transmission but selective impairment in synaptic potentiation maintenance. We identify muskelin at excitatory synapses and highlight its role in regulating dendritic spine actin stability. Our findings point to aberrant spine actin modulation and changes in glutamatergic synaptic function as critical mechanisms that contribute to the neurobehavioral phenotype arising from <jats:italic>Mkln1</jats:italic> ablation.","lang":"eng"}],"external_id":{"isi":["000811777900003"]},"department":[{"_id":"PreCl"}],"oa":1,"corr_author":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file_date_updated":"2023-01-27T08:23:46Z","language":[{"iso":"eng"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"article_processing_charge":"No","title":"Muskelin regulates actin-dependent synaptic changes and intrinsic brain activity relevant to behavioral and cognitive processes","date_published":"2022-06-15T00:00:00Z","intvolume":"         5","_id":"12224","type":"journal_article","has_accepted_license":"1"},{"scopus_import":"1","page":"2290-2304.e7","publication_status":"published","citation":{"ieee":"N. Hino <i>et al.</i>, “A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration,” <i>Developmental Cell</i>, vol. 57, no. 19. Elsevier, p. 2290–2304.e7, 2022.","short":"N. Hino, K. Matsuda, Y. Jikko, G. Maryu, K. Sakai, R. Imamura, S. Tsukiji, K. Aoki, K. Terai, T. Hirashima, X. Trepat, M. Matsuda, Developmental Cell 57 (2022) 2290–2304.e7.","apa":"Hino, N., Matsuda, K., Jikko, Y., Maryu, G., Sakai, K., Imamura, R., … Matsuda, M. (2022). A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2022.09.003\">https://doi.org/10.1016/j.devcel.2022.09.003</a>","mla":"Hino, Naoya, et al. “A Feedback Loop between Lamellipodial Extension and HGF-ERK Signaling Specifies Leader Cells during Collective Cell Migration.” <i>Developmental Cell</i>, vol. 57, no. 19, Elsevier, 2022, p. 2290–2304.e7, doi:<a href=\"https://doi.org/10.1016/j.devcel.2022.09.003\">10.1016/j.devcel.2022.09.003</a>.","chicago":"Hino, Naoya, Kimiya Matsuda, Yuya Jikko, Gembu Maryu, Katsuya Sakai, Ryu Imamura, Shinya Tsukiji, et al. “A Feedback Loop between Lamellipodial Extension and HGF-ERK Signaling Specifies Leader Cells during Collective Cell Migration.” <i>Developmental Cell</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.devcel.2022.09.003\">https://doi.org/10.1016/j.devcel.2022.09.003</a>.","ista":"Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, Tsukiji S, Aoki K, Terai K, Hirashima T, Trepat X, Matsuda M. 2022. A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. Developmental Cell. 57(19), 2290–2304.e7.","ama":"Hino N, Matsuda K, Jikko Y, et al. A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. <i>Developmental Cell</i>. 2022;57(19):2290-2304.e7. doi:<a href=\"https://doi.org/10.1016/j.devcel.2022.09.003\">10.1016/j.devcel.2022.09.003</a>"},"isi":1,"volume":57,"publisher":"Elsevier","day":"01","author":[{"first_name":"Naoya","id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","full_name":"Hino, Naoya","last_name":"Hino"},{"last_name":"Matsuda","full_name":"Matsuda, Kimiya","first_name":"Kimiya"},{"first_name":"Yuya","last_name":"Jikko","full_name":"Jikko, Yuya"},{"full_name":"Maryu, Gembu","last_name":"Maryu","first_name":"Gembu"},{"full_name":"Sakai, Katsuya","last_name":"Sakai","first_name":"Katsuya"},{"first_name":"Ryu","full_name":"Imamura, Ryu","last_name":"Imamura"},{"full_name":"Tsukiji, Shinya","last_name":"Tsukiji","first_name":"Shinya"},{"first_name":"Kazuhiro","full_name":"Aoki, Kazuhiro","last_name":"Aoki"},{"first_name":"Kenta","full_name":"Terai, Kenta","last_name":"Terai"},{"full_name":"Hirashima, Tsuyoshi","last_name":"Hirashima","first_name":"Tsuyoshi"},{"full_name":"Trepat, Xavier","last_name":"Trepat","first_name":"Xavier"},{"first_name":"Michiyuki","last_name":"Matsuda","full_name":"Matsuda, Michiyuki"}],"quality_controlled":"1","doi":"10.1016/j.devcel.2022.09.003","OA_place":"publisher","keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"ddc":["570"],"issue":"19","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2022.09.003","open_access":"1"}],"status":"public","oa_version":"Published Version","article_type":"original","month":"10","external_id":{"isi":["000898428700006"],"pmid":["36174555"]},"abstract":[{"text":"Upon the initiation of collective cell migration, the cells at the free edge are specified as leader cells; however, the mechanism underlying the leader cell specification remains elusive. Here, we show that lamellipodial extension after the release from mechanical confinement causes sustained extracellular signal-regulated kinase (ERK) activation and underlies the leader cell specification. Live-imaging of Madin-Darby canine kidney (MDCK) cells and mouse epidermis through the use of Förster resonance energy transfer (FRET)-based biosensors showed that leader cells exhibit sustained ERK activation in a hepatocyte growth factor (HGF)-dependent manner. Meanwhile, follower cells exhibit oscillatory ERK activation waves in an epidermal growth factor (EGF) signaling-dependent manner. Lamellipodial extension at the free edge increases the cellular sensitivity to HGF. The HGF-dependent ERK activation, in turn, promotes lamellipodial extension, thereby forming a positive feedback loop between cell extension and ERK activation and specifying the cells at the free edge as the leader cells. Our findings show that the integration of physical and biochemical cues underlies the leader cell specification during collective cell migration.","lang":"eng"}],"date_updated":"2026-06-18T17:25:21Z","year":"2022","publication":"Developmental Cell","date_created":"2023-01-16T09:51:39Z","acknowledgement":"We thank the members of the Matsuda Laboratory for their helpful discussion and encouragement, and we thank K. Hirano and K. Takakura for their technical assistance. This work was supported by the Kyoto University Live Imaging Center. Financial support was provided in the form of JSPS KAKENHI grants (nos. 17J02107 and 20K22653 to N.H., and 20H05898 and 19H00993 to M.M.), a JST CREST grant (no. JPMJCR1654 to M.M.), a Moonshot R&D grant (no. JPMJPS2022-11 to M.M.), Generalitat de Catalunya and the CERCA Programme (no. SGR-2017-01602 to X.T.), MICCINN/FEDER (no. PGC2018-099645-B-I00 to X.T.), and European Research Council (no. Adv-883739 to X.T.). IBEC is a recipient of a Severo Ochoa Award of Excellence from the MINECO. This work was partly supported by an Extramural Collaborative Research Grant of Cancer Research Institute, Kanazawa University.","publication_identifier":{"issn":["1534-5807"]},"oa":1,"corr_author":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CaHe"}],"date_published":"2022-10-01T00:00:00Z","pmid":1,"title":"A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration","article_processing_charge":"No","language":[{"iso":"eng"}],"OA_type":"free access","_id":"12238","type":"journal_article","intvolume":"        57"},{"department":[{"_id":"ToBo"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"publication_identifier":{"eissn":["1744-4292"]},"acknowledgement":"This work was in part supported by Human Frontier Science Program GrantRGP0042/2013, Marie Curie Career Integration Grant303507, AustrianScience Fund (FWF) Grant P27201-B22, and German Research Foundation(DFG) Collaborative Research Center (SFB)1310to TB. SAA was supportedby the European Union’s Horizon2020Research and Innovation Programunder the Marie Skłodowska-Curie Grant agreement No707352. We wouldlike to thank the Bollenbach group for regular fruitful discussions. We areparticularly thankful for the technical assistance of Booshini Fernando andfor discussions of the theoretical aspects with Gerrit Ansmann. We areindebted to Bor Kavˇciˇc for invaluable advice, help with setting up theluciferase-based growth monitoring system, and for sharing plasmids. Weacknowledge the IST Austria Miba Machine Shop for their support inbuilding a housing for the stacker of the plate reader, which enabled thehigh-throughput luciferase-based experiments. We are grateful to RosalindAllen, Bor Kavˇciˇc and Dor Russ for feedback on the manuscript. Open Accessfunding enabled and organized by Projekt DEAL.","publication":"Molecular Systems Biology","date_created":"2023-01-16T09:58:34Z","date_updated":"2025-06-11T14:10:18Z","year":"2022","abstract":[{"lang":"eng","text":"Dose–response relationships are a general concept for quantitatively describing biological systems across multiple scales, from the molecular to the whole-cell level. A clinically relevant example is the bacterial growth response to antibiotics, which is routinely characterized by dose–response curves. The shape of the dose–response curve varies drastically between antibiotics and plays a key role in treatment, drug interactions, and resistance evolution. However, the mechanisms shaping the dose–response curve remain largely unclear. Here, we show in Escherichia coli that the distinctively shallow dose–response curve of the antibiotic trimethoprim is caused by a negative growth-mediated feedback loop: Trimethoprim slows growth, which in turn weakens the effect of this antibiotic. At the molecular level, this feedback is caused by the upregulation of the drug target dihydrofolate reductase (FolA/DHFR). We show that this upregulation is not a specific response to trimethoprim but follows a universal trend line that depends primarily on the growth rate, irrespective of its cause. Rewiring the feedback loop alters the dose–response curve in a predictable manner, which we corroborate using a mathematical model of cellular resource allocation and growth. Our results indicate that growth-mediated feedback loops may shape drug responses more generally and could be exploited to design evolutionary traps that enable selection against drug resistance."}],"external_id":{"isi":["000856482800001"],"pmid":["36124745"]},"intvolume":"        18","_id":"12261","type":"journal_article","acknowledged_ssus":[{"_id":"M-Shop"}],"has_accepted_license":"1","language":[{"iso":"eng"}],"file_date_updated":"2023-01-30T09:49:55Z","article_processing_charge":"No","title":"Growth‐mediated negative feedback shapes quantitative antibiotic response","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"pmid":1,"date_published":"2022-09-01T00:00:00Z","author":[{"full_name":"Angermayr, Andreas","last_name":"Angermayr","orcid":"0000-0001-8619-2223","id":"4677C796-F248-11E8-B48F-1D18A9856A87","first_name":"Andreas"},{"first_name":"Tin Yau","last_name":"Pang","full_name":"Pang, Tin Yau"},{"first_name":"Guillaume","full_name":"Chevereau, Guillaume","last_name":"Chevereau"},{"id":"39B66846-F248-11E8-B48F-1D18A9856A87","first_name":"Karin","full_name":"Mitosch, Karin","last_name":"Mitosch"},{"full_name":"Lercher, Martin J","last_name":"Lercher","first_name":"Martin J"},{"full_name":"Bollenbach, Mark Tobias","last_name":"Bollenbach","orcid":"0000-0003-4398-476X","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","first_name":"Mark Tobias"}],"day":"01","publisher":"Embo Press","volume":18,"file":[{"file_size":1098812,"access_level":"open_access","relation":"main_file","file_name":"2022_MolecularSystemsBio_Angermayr.pdf","date_created":"2023-01-30T09:49:55Z","date_updated":"2023-01-30T09:49:55Z","file_id":"12446","content_type":"application/pdf","creator":"dernst","success":1,"checksum":"8b1d8f5ea20c8408acf466435fb6ae01"}],"citation":{"short":"A. Angermayr, T.Y. Pang, G. Chevereau, K. Mitosch, M.J. Lercher, M.T. Bollenbach, Molecular Systems Biology 18 (2022).","apa":"Angermayr, A., Pang, T. Y., Chevereau, G., Mitosch, K., Lercher, M. J., &#38; Bollenbach, M. T. (2022). Growth‐mediated negative feedback shapes quantitative antibiotic response. <i>Molecular Systems Biology</i>. Embo Press. <a href=\"https://doi.org/10.15252/msb.202110490\">https://doi.org/10.15252/msb.202110490</a>","mla":"Angermayr, Andreas, et al. “Growth‐mediated Negative Feedback Shapes Quantitative Antibiotic Response.” <i>Molecular Systems Biology</i>, vol. 18, no. 9, e10490, Embo Press, 2022, doi:<a href=\"https://doi.org/10.15252/msb.202110490\">10.15252/msb.202110490</a>.","ama":"Angermayr A, Pang TY, Chevereau G, Mitosch K, Lercher MJ, Bollenbach MT. Growth‐mediated negative feedback shapes quantitative antibiotic response. <i>Molecular Systems Biology</i>. 2022;18(9). doi:<a href=\"https://doi.org/10.15252/msb.202110490\">10.15252/msb.202110490</a>","ista":"Angermayr A, Pang TY, Chevereau G, Mitosch K, Lercher MJ, Bollenbach MT. 2022. Growth‐mediated negative feedback shapes quantitative antibiotic response. Molecular Systems Biology. 18(9), e10490.","chicago":"Angermayr, Andreas, Tin Yau Pang, Guillaume Chevereau, Karin Mitosch, Martin J Lercher, and Mark Tobias Bollenbach. “Growth‐mediated Negative Feedback Shapes Quantitative Antibiotic Response.” <i>Molecular Systems Biology</i>. Embo Press, 2022. <a href=\"https://doi.org/10.15252/msb.202110490\">https://doi.org/10.15252/msb.202110490</a>.","ieee":"A. Angermayr, T. Y. Pang, G. Chevereau, K. Mitosch, M. J. Lercher, and M. T. Bollenbach, “Growth‐mediated negative feedback shapes quantitative antibiotic response,” <i>Molecular Systems Biology</i>, vol. 18, no. 9. Embo Press, 2022."},"isi":1,"publication_status":"published","scopus_import":"1","month":"09","article_type":"original","oa_version":"Published Version","status":"public","ddc":["570"],"issue":"9","article_number":"e10490","keyword":["Applied Mathematics","Computational Theory and Mathematics","General Agricultural and Biological Sciences","General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","Information Systems"],"doi":"10.15252/msb.202110490","quality_controlled":"1"},{"file_date_updated":"2023-01-30T11:50:53Z","language":[{"iso":"eng"}],"title":"Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling","article_processing_charge":"No","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"pmid":1,"date_published":"2022-09-15T00:00:00Z","intvolume":"        11","_id":"12288","type":"journal_article","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"has_accepted_license":"1","publication_identifier":{"eissn":["2050-084X"]},"acknowledgement":"We thank F Marr for technical assistance, A Murray for RVdG-CVS-N2c viruses and Neuro2A packaging cell-lines and J Watson for reading the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Imaging and Optics Facility (IOF) and the Preclinical Facility (PCF). This project was funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (ERC advanced grant No 692692, PJ, ERC starting grant No 756502, MJ), the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award, PJ), the Human Frontier Science Program (LT000256/2018-L, AS) and EMBO (ALTF 1098-2017, AS).","date_created":"2023-01-16T10:04:15Z","publication":"eLife","year":"2022","date_updated":"2025-04-15T08:29:05Z","abstract":[{"text":"To understand the function of neuronal circuits, it is crucial to disentangle the connectivity patterns within the network. However, most tools currently used to explore connectivity have low throughput, low selectivity, or limited accessibility. Here, we report the development of an improved packaging system for the production of the highly neurotropic RVdGenvA-CVS-N2c rabies viral vectors, yielding titers orders of magnitude higher with no background contamination, at a fraction of the production time, while preserving the efficiency of transsynaptic labeling. Along with the production pipeline, we developed suites of ‘starter’ AAV and bicistronic RVdG-CVS-N2c vectors, enabling retrograde labeling from a wide range of neuronal populations, tailored for diverse experimental requirements. We demonstrate the power and flexibility of the new system by uncovering hidden local and distal inhibitory connections in the mouse hippocampal formation and by imaging the functional properties of a cortical microcircuit across weeks. Our novel production pipeline provides a convenient approach to generate new rabies vectors, while our toolkit flexibly and efficiently expands the current capacity to label, manipulate and image the neuronal activity of interconnected neuronal circuits in vitro and in vivo.","lang":"eng"}],"external_id":{"isi":["000892204300001"],"pmid":["36040301"]},"department":[{"_id":"MaJö"},{"_id":"PeJo"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","corr_author":"1","oa":1,"doi":"10.7554/elife.79848","quality_controlled":"1","month":"09","article_type":"original","oa_version":"Published Version","status":"public","ddc":["570"],"article_number":"79848","keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","General Medicine","General Neuroscience"],"isi":1,"file":[{"access_level":"open_access","relation":"main_file","file_size":8506811,"file_name":"2022_eLife_Sumser.pdf","date_updated":"2023-01-30T11:50:53Z","file_id":"12463","date_created":"2023-01-30T11:50:53Z","content_type":"application/pdf","creator":"dernst","success":1,"checksum":"5a2a65e3e7225090c3d8199f3bbd7b7b"}],"citation":{"ista":"Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. 2022. Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling. eLife. 11, 79848.","ama":"Sumser AL, Jösch MA, Jonas PM, Ben Simon Y. Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling. <i>eLife</i>. 2022;11. doi:<a href=\"https://doi.org/10.7554/elife.79848\">10.7554/elife.79848</a>","mla":"Sumser, Anton L., et al. “Fast, High-Throughput Production of Improved Rabies Viral Vectors for Specific, Efficient and Versatile Transsynaptic Retrograde Labeling.” <i>ELife</i>, vol. 11, 79848, eLife Sciences Publications, 2022, doi:<a href=\"https://doi.org/10.7554/elife.79848\">10.7554/elife.79848</a>.","chicago":"Sumser, Anton L, Maximilian A Jösch, Peter M Jonas, and Yoav Ben Simon. “Fast, High-Throughput Production of Improved Rabies Viral Vectors for Specific, Efficient and Versatile Transsynaptic Retrograde Labeling.” <i>ELife</i>. eLife Sciences Publications, 2022. <a href=\"https://doi.org/10.7554/elife.79848\">https://doi.org/10.7554/elife.79848</a>.","short":"A.L. Sumser, M.A. Jösch, P.M. Jonas, Y. Ben Simon, ELife 11 (2022).","apa":"Sumser, A. L., Jösch, M. A., Jonas, P. M., &#38; Ben Simon, Y. (2022). Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.79848\">https://doi.org/10.7554/elife.79848</a>","ieee":"A. L. Sumser, M. A. Jösch, P. M. Jonas, and Y. Ben Simon, “Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling,” <i>eLife</i>, vol. 11. eLife Sciences Publications, 2022."},"publication_status":"published","ec_funded":1,"scopus_import":"1","author":[{"first_name":"Anton L","id":"3320A096-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4792-1881","full_name":"Sumser, Anton L","last_name":"Sumser"},{"id":"2BD278E6-F248-11E8-B48F-1D18A9856A87","first_name":"Maximilian A","orcid":"0000-0002-3937-1330","full_name":"Jösch, Maximilian A","last_name":"Jösch"},{"orcid":"0000-0001-5001-4804","first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Jonas, Peter M","last_name":"Jonas"},{"last_name":"Ben Simon","full_name":"Ben Simon, Yoav","first_name":"Yoav","id":"43DF3136-F248-11E8-B48F-1D18A9856A87"}],"day":"15","project":[{"grant_number":"692692","call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","name":"Biophysics and circuit function of a giant cortical glutamatergic synapse"},{"name":"Circuits of Visual Attention","call_identifier":"H2020","grant_number":"756502","_id":"2634E9D2-B435-11E9-9278-68D0E5697425"},{"_id":"25C5A090-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"Z00312","name":"Synaptic communication in neuronal microcircuits"},{"name":"Neuronal networks of salience and spatial detection in the murine superior colliculus","_id":"266D407A-B435-11E9-9278-68D0E5697425","grant_number":"LT000256"},{"grant_number":"ALTF 1098-2017","_id":"264FEA02-B435-11E9-9278-68D0E5697425","name":"Connecting sensory with motor processing in the superior colliculus"}],"publisher":"eLife Sciences Publications","volume":11},{"scopus_import":"1","publication_status":"published","page":"2240-2251","citation":{"ieee":"S. He and X. Feng, “DNA methylation dynamics during germline development,” <i>Journal of Integrative Plant Biology</i>, vol. 64, no. 12. Wiley, pp. 2240–2251, 2022.","mla":"He, Shengbo, and Xiaoqi Feng. “DNA Methylation Dynamics during Germline Development.” <i>Journal of Integrative Plant Biology</i>, vol. 64, no. 12, Wiley, 2022, pp. 2240–51, doi:<a href=\"https://doi.org/10.1111/jipb.13422\">10.1111/jipb.13422</a>.","ama":"He S, Feng X. DNA methylation dynamics during germline development. <i>Journal of Integrative Plant Biology</i>. 2022;64(12):2240-2251. doi:<a href=\"https://doi.org/10.1111/jipb.13422\">10.1111/jipb.13422</a>","ista":"He S, Feng X. 2022. DNA methylation dynamics during germline development. Journal of Integrative Plant Biology. 64(12), 2240–2251.","chicago":"He, Shengbo, and Xiaoqi Feng. “DNA Methylation Dynamics during Germline Development.” <i>Journal of Integrative Plant Biology</i>. Wiley, 2022. <a href=\"https://doi.org/10.1111/jipb.13422\">https://doi.org/10.1111/jipb.13422</a>.","short":"S. He, X. Feng, Journal of Integrative Plant Biology 64 (2022) 2240–2251.","apa":"He, S., &#38; Feng, X. (2022). DNA methylation dynamics during germline development. <i>Journal of Integrative Plant Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/jipb.13422\">https://doi.org/10.1111/jipb.13422</a>"},"volume":64,"publisher":"Wiley","day":"07","author":[{"full_name":"He, Shengbo","last_name":"He","first_name":"Shengbo"},{"orcid":"0000-0002-4008-1234","id":"e0164712-22ee-11ed-b12a-d80fcdf35958","first_name":"Xiaoqi","last_name":"Feng","full_name":"Feng, Xiaoqi"}],"doi":"10.1111/jipb.13422","quality_controlled":"1","issue":"12","keyword":["Plant Science","General Biochemistry","Genetics and Molecular Biology","Biochemistry"],"status":"public","main_file_link":[{"url":"https://doi.org/10.1111/jipb.13422","open_access":"1"}],"oa_version":"Published Version","month":"12","article_type":"review","external_id":{"pmid":["36478632"]},"abstract":[{"lang":"eng","text":"DNA methylation plays essential homeostatic functions in eukaryotic genomes. In animals, DNA methylation is also developmentally regulated and, in turn, regulates development. In the past two decades, huge research effort has endorsed the understanding that DNA methylation plays a similar role in plant development, especially during sexual reproduction. The power of whole-genome sequencing and cell isolation techniques, as well as bioinformatics tools, have enabled recent studies to reveal dynamic changes in DNA methylation during germline development. Furthermore, the combination of these technological advances with genetics, developmental biology and cell biology tools has revealed functional methylation reprogramming events that control gene and transposon activities in flowering plant germlines. In this review, we discuss the major advances in our knowledge of DNA methylation dynamics during male and female germline development in flowering plants."}],"date_created":"2023-02-23T09:15:57Z","publication":"Journal of Integrative Plant Biology","date_updated":"2024-10-14T12:03:14Z","year":"2022","publication_identifier":{"eissn":["1744-7909"],"issn":["1672-9072"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"extern":"1","department":[{"_id":"XiFe"}],"pmid":1,"date_published":"2022-12-07T00:00:00Z","title":"DNA methylation dynamics during germline development","article_processing_charge":"No","language":[{"iso":"eng"}],"type":"journal_article","_id":"12670","intvolume":"        64"},{"citation":{"ieee":"J. P. K. Bravo, C. Aparicio-Maldonado, F. L. Nobrega, S. J. J. Brouns, and D. W. Taylor, “Structural basis for broad anti-phage immunity by DISARM,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.","chicago":"Bravo, Jack Peter Kelly, Cristian Aparicio-Maldonado, Franklin L. Nobrega, Stan J. J. Brouns, and David W. Taylor. “Structural Basis for Broad Anti-Phage Immunity by DISARM.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-30673-1\">https://doi.org/10.1038/s41467-022-30673-1</a>.","ama":"Bravo JPK, Aparicio-Maldonado C, Nobrega FL, Brouns SJJ, Taylor DW. Structural basis for broad anti-phage immunity by DISARM. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-30673-1\">10.1038/s41467-022-30673-1</a>","mla":"Bravo, Jack Peter Kelly, et al. “Structural Basis for Broad Anti-Phage Immunity by DISARM.” <i>Nature Communications</i>, vol. 13, 2987, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-30673-1\">10.1038/s41467-022-30673-1</a>.","ista":"Bravo JPK, Aparicio-Maldonado C, Nobrega FL, Brouns SJJ, Taylor DW. 2022. Structural basis for broad anti-phage immunity by DISARM. Nature Communications. 13, 2987.","short":"J.P.K. Bravo, C. Aparicio-Maldonado, F.L. Nobrega, S.J.J. Brouns, D.W. Taylor, Nature Communications 13 (2022).","apa":"Bravo, J. P. K., Aparicio-Maldonado, C., Nobrega, F. L., Brouns, S. J. J., &#38; Taylor, D. W. (2022). Structural basis for broad anti-phage immunity by DISARM. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-30673-1\">https://doi.org/10.1038/s41467-022-30673-1</a>"},"publication_status":"published","scopus_import":"1","author":[{"id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","first_name":"Jack Peter Kelly","orcid":"0000-0003-0456-0753","full_name":"Bravo, Jack Peter Kelly","last_name":"Bravo"},{"last_name":"Aparicio-Maldonado","full_name":"Aparicio-Maldonado, Cristian","first_name":"Cristian"},{"last_name":"Nobrega","full_name":"Nobrega, Franklin L.","first_name":"Franklin L."},{"first_name":"Stan J. J.","full_name":"Brouns, Stan J. J.","last_name":"Brouns"},{"full_name":"Taylor, David W.","last_name":"Taylor","first_name":"David W."}],"day":"27","publisher":"Springer Nature","volume":13,"quality_controlled":"1","doi":"10.1038/s41467-022-30673-1","article_type":"original","month":"05","oa_version":"Published Version","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41467-022-30673-1"}],"status":"public","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"article_number":"2987","publication_identifier":{"issn":["2041-1723"]},"year":"2022","date_updated":"2024-06-04T06:16:38Z","publication":"Nature Communications","date_created":"2024-03-20T10:41:59Z","abstract":[{"text":"In the evolutionary arms race against phage, bacteria have assembled a diverse arsenal of antiviral immune strategies. While the recently discovered DISARM (Defense Island System Associated with Restriction-Modification) systems can provide protection against a wide range of phage, the molecular mechanisms that underpin broad antiviral targeting but avoiding autoimmunity remain enigmatic. Here, we report cryo-EM structures of the core DISARM complex, DrmAB, both alone and in complex with an unmethylated phage DNA mimetic. These structures reveal that DrmAB core complex is autoinhibited by a trigger loop (TL) within DrmA and binding to DNA substrates containing a 5′ overhang dislodges the TL, initiating a long-range structural rearrangement for DrmAB activation. Together with structure-guided in vivo studies, our work provides insights into the mechanism of phage DNA recognition and specific activation of this widespread antiviral defense system.","lang":"eng"}],"external_id":{"pmid":["35624106"]},"extern":"1","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"article_processing_charge":"Yes","title":"Structural basis for broad anti-phage immunity by DISARM","date_published":"2022-05-27T00:00:00Z","pmid":1,"intvolume":"        13","type":"journal_article","_id":"15133"},{"publication_status":"published","citation":{"ieee":"E. A. Schwartz <i>et al.</i>, “Structural rearrangements allow nucleic acid discrimination by type I-D Cascade,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.","short":"E.A. Schwartz, T.M. McBride, J.P.K. Bravo, D. Wrapp, P.C. Fineran, R.D. Fagerlund, D.W. Taylor, Nature Communications 13 (2022).","apa":"Schwartz, E. A., McBride, T. M., Bravo, J. P. K., Wrapp, D., Fineran, P. C., Fagerlund, R. D., &#38; Taylor, D. W. (2022). Structural rearrangements allow nucleic acid discrimination by type I-D Cascade. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-30402-8\">https://doi.org/10.1038/s41467-022-30402-8</a>","ama":"Schwartz EA, McBride TM, Bravo JPK, et al. Structural rearrangements allow nucleic acid discrimination by type I-D Cascade. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-30402-8\">10.1038/s41467-022-30402-8</a>","ista":"Schwartz EA, McBride TM, Bravo JPK, Wrapp D, Fineran PC, Fagerlund RD, Taylor DW. 2022. Structural rearrangements allow nucleic acid discrimination by type I-D Cascade. Nature Communications. 13, 2829.","chicago":"Schwartz, Evan A., Tess M. McBride, Jack Peter Kelly Bravo, Daniel Wrapp, Peter C. Fineran, Robert D. Fagerlund, and David W. Taylor. “Structural Rearrangements Allow Nucleic Acid Discrimination by Type I-D Cascade.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-30402-8\">https://doi.org/10.1038/s41467-022-30402-8</a>.","mla":"Schwartz, Evan A., et al. “Structural Rearrangements Allow Nucleic Acid Discrimination by Type I-D Cascade.” <i>Nature Communications</i>, vol. 13, 2829, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-30402-8\">10.1038/s41467-022-30402-8</a>."},"scopus_import":"1","day":"20","author":[{"full_name":"Schwartz, Evan A.","last_name":"Schwartz","first_name":"Evan A."},{"full_name":"McBride, Tess M.","last_name":"McBride","first_name":"Tess M."},{"orcid":"0000-0003-0456-0753","first_name":"Jack Peter Kelly","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","last_name":"Bravo","full_name":"Bravo, Jack Peter Kelly"},{"first_name":"Daniel","last_name":"Wrapp","full_name":"Wrapp, Daniel"},{"first_name":"Peter C.","last_name":"Fineran","full_name":"Fineran, Peter C."},{"first_name":"Robert D.","full_name":"Fagerlund, Robert D.","last_name":"Fagerlund"},{"first_name":"David W.","last_name":"Taylor","full_name":"Taylor, David W."}],"volume":13,"publisher":"Springer Nature","quality_controlled":"1","doi":"10.1038/s41467-022-30402-8","oa_version":"Published Version","article_type":"original","month":"05","article_number":"2829","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"status":"public","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41467-022-30402-8"}],"year":"2022","date_updated":"2024-06-04T06:14:28Z","publication":"Nature Communications","date_created":"2024-03-20T10:42:05Z","publication_identifier":{"issn":["2041-1723"]},"external_id":{"pmid":["35595728"]},"abstract":[{"lang":"eng","text":"CRISPR-Cas systems are adaptive immune systems that protect prokaryotes from foreign nucleic acids, such as bacteriophages. Two of the most prevalent CRISPR-Cas systems include type I and type III. Interestingly, the type I-D interference proteins contain characteristic features of both type I and type III systems. Here, we present the structures of type I-D Cascade bound to both a double-stranded (ds)DNA and a single-stranded (ss)RNA target at 2.9 and 3.1 Å, respectively. We show that type I-D Cascade is capable of specifically binding ssRNA and reveal how PAM recognition of dsDNA targets initiates long-range structural rearrangements that likely primes Cas10d for Cas3′ binding and subsequent non-target strand DNA cleavage. These structures allow us to model how binding of the anti-CRISPR protein AcrID1 likely blocks target dsDNA binding via competitive inhibition of the DNA substrate engagement with the Cas10d active site. This work elucidates the unique mechanisms used by type I-D Cascade for discrimination of single-stranded and double stranded targets. Thus, our data supports a model for the hybrid nature of this complex with features of type III and type I systems."}],"extern":"1","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"date_published":"2022-05-20T00:00:00Z","pmid":1,"title":"Structural rearrangements allow nucleic acid discrimination by type I-D Cascade","article_processing_charge":"Yes","intvolume":"        13","type":"journal_article","_id":"15134"},{"doi":"10.1098/rstb.2021.0009","quality_controlled":"1","status":"public","issue":"1848","ddc":["570"],"keyword":["General Agricultural and Biological Sciences","General Biochemistry","Genetics and Molecular Biology"],"month":"04","article_type":"original","oa_version":"Published Version","scopus_import":"1","file":[{"file_name":"2022_PhilosophicalTransactionsRSB_Barton.pdf","access_level":"open_access","file_size":1349672,"relation":"main_file","content_type":"application/pdf","date_created":"2022-08-02T06:14:32Z","date_updated":"2022-08-02T06:14:32Z","file_id":"11719","creator":"dernst","checksum":"3b0243738f01bf3c07e0d7e8dc64f71d","success":1}],"isi":1,"citation":{"mla":"Barton, Nicholas H., and Oluwafunmilola O. Olusanya. “The Response of a Metapopulation to a Changing Environment.” <i>Philosophical Transactions of the Royal Society B: Biological Sciences</i>, vol. 377, no. 1848, The Royal Society, 2022, doi:<a href=\"https://doi.org/10.1098/rstb.2021.0009\">10.1098/rstb.2021.0009</a>.","ama":"Barton NH, Olusanya OO. The response of a metapopulation to a changing environment. <i>Philosophical Transactions of the Royal Society B: Biological Sciences</i>. 2022;377(1848). doi:<a href=\"https://doi.org/10.1098/rstb.2021.0009\">10.1098/rstb.2021.0009</a>","ista":"Barton NH, Olusanya OO. 2022. The response of a metapopulation to a changing environment. Philosophical Transactions of the Royal Society B: Biological Sciences. 377(1848).","chicago":"Barton, Nicholas H, and Oluwafunmilola O Olusanya. “The Response of a Metapopulation to a Changing Environment.” <i>Philosophical Transactions of the Royal Society B: Biological Sciences</i>. The Royal Society, 2022. <a href=\"https://doi.org/10.1098/rstb.2021.0009\">https://doi.org/10.1098/rstb.2021.0009</a>.","apa":"Barton, N. H., &#38; Olusanya, O. O. (2022). The response of a metapopulation to a changing environment. <i>Philosophical Transactions of the Royal Society B: Biological Sciences</i>. The Royal Society. <a href=\"https://doi.org/10.1098/rstb.2021.0009\">https://doi.org/10.1098/rstb.2021.0009</a>","short":"N.H. Barton, O.O. Olusanya, Philosophical Transactions of the Royal Society B: Biological Sciences 377 (2022).","ieee":"N. H. Barton and O. O. Olusanya, “The response of a metapopulation to a changing environment,” <i>Philosophical Transactions of the Royal Society B: Biological Sciences</i>, vol. 377, no. 1848. The Royal Society, 2022."},"publication_status":"published","project":[{"grant_number":"P32896","_id":"c08d3278-5a5b-11eb-8a69-fdb09b55f4b8","name":"Causes and consequences of population fragmentation"}],"publisher":"The Royal Society","volume":377,"author":[{"last_name":"Barton","full_name":"Barton, Nicholas H","id":"4880FE40-F248-11E8-B48F-1D18A9856A87","first_name":"Nicholas H","orcid":"0000-0002-8548-5240"},{"full_name":"Olusanya, Oluwafunmilola O","last_name":"Olusanya","first_name":"Oluwafunmilola O","id":"41AD96DC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1971-8314"}],"day":"11","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"14711"}]},"title":"The response of a metapopulation to a changing environment","article_processing_charge":"No","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"pmid":1,"date_published":"2022-04-11T00:00:00Z","file_date_updated":"2022-08-02T06:14:32Z","language":[{"iso":"eng"}],"type":"journal_article","_id":"10787","has_accepted_license":"1","intvolume":"       377","abstract":[{"text":"A species distributed across diverse environments may adapt to local conditions. We ask how quickly such a species changes its range in response to changed conditions. Szép et al. (Szép E, Sachdeva H, Barton NH. 2021 Polygenic local adaptation in metapopulations: a stochastic eco-evolutionary model. Evolution75, 1030–1045 (doi:10.1111/evo.14210)) used the infinite island model to find the stationary distribution of allele frequencies and deme sizes. We extend this to find how a metapopulation responds to changes in carrying capacity, selection strength, or migration rate when deme sizes are fixed. We further develop a ‘fixed-state’ approximation. Under this approximation, polymorphism is only possible for a narrow range of habitat proportions when selection is weak compared to drift, but for a much wider range otherwise. When rates of selection or migration relative to drift change in a single deme of the metapopulation, the population takes a time of order m−1 to reach the new equilibrium. However, even with many loci, there can be substantial fluctuations in net adaptation, because at each locus, alleles randomly get lost or fixed. Thus, in a finite metapopulation, variation may gradually be lost by chance, even if it would persist in an infinite metapopulation. When conditions change across the whole metapopulation, there can be rapid change, which is predicted well by the fixed-state approximation. This work helps towards an understanding of how metapopulations extend their range across diverse environments.\r\nThis article is part of the theme issue ‘Species’ ranges in the face of changing environments (Part II)’.","lang":"eng"}],"external_id":{"isi":["000758140300001"],"pmid":["35184588"]},"publication_identifier":{"eissn":["1471-2970"],"issn":["0962-8436"]},"acknowledgement":"This research was partly funded by the Austrian Science Fund (FWF) [FWF P-32896B].","publication":"Philosophical Transactions of the Royal Society B: Biological Sciences","date_created":"2022-02-21T16:08:10Z","date_updated":"2026-04-07T12:54:28Z","year":"2022","corr_author":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa":1,"department":[{"_id":"GradSch"},{"_id":"NiBa"}]},{"external_id":{"pmid":["33711252"],"isi":["000654652200002"]},"abstract":[{"lang":"eng","text":"Hematopoietic-specific protein 1 (Hem1) is an essential subunit of the WAVE regulatory complex (WRC) in immune cells. WRC is crucial for Arp2/3 complex activation and the protrusion of branched actin filament networks. Moreover, Hem1 loss of function in immune cells causes autoimmune diseases in humans. Here, we show that genetic removal of Hem1 in macrophages diminishes frequency and efficacy of phagocytosis as well as phagocytic cup formation in addition to defects in lamellipodial protrusion and migration. Moreover, Hem1-null macrophages displayed strong defects in cell adhesion despite unaltered podosome formation and concomitant extracellular matrix degradation. Specifically, dynamics of both adhesion and de-adhesion as well as concomitant phosphorylation of paxillin and focal adhesion kinase (FAK) were significantly compromised. Accordingly, disruption of WRC function in non-hematopoietic cells coincided with both defects in adhesion turnover and altered FAK and paxillin phosphorylation. Consistently, platelets exhibited reduced adhesion and diminished integrin αIIbβ3 activation upon WRC removal. Interestingly, adhesion phenotypes, but not lamellipodia formation, were partially rescued by small molecule activation of FAK. A full rescue of the phenotype, including lamellipodia formation, required not only the presence of WRCs but also their binding to and activation by Rac. Collectively, our results uncover that WRC impacts on integrin-dependent processes in a FAK-dependent manner, controlling formation and dismantling of adhesions, relevant for properly grabbing onto extracellular surfaces and particles during cell edge expansion, like in migration or phagocytosis."}],"publication":"Current Biology","date_created":"2022-03-08T07:51:04Z","year":"2021","date_updated":"2023-08-17T07:01:14Z","publication_identifier":{"issn":["0960-9822"]},"acknowledgement":"We are grateful to Silvia Prettin, Ina Schleicher, and Petra Hagendorff for expert technical assistance; David Dettbarn for animal keeping and breeding; and Lothar Gröbe and Maria Höxter for cell sorting. We also thank Werner Tegge for peptides and Giorgio Scita for antibodies. This work was supported, in part, by the Deutsche Forschungsgemeinschaft (DFG), Priority Programm SPP1150 (to T.E.B.S., K.R., and M. Sixt), and by DFG grant GRK2223/1 (to K.R.). T.E.B.S. acknowledges support by the Helmholtz Society through HGF impulse fund W2/W3-066 and M. Schnoor by the Mexican Council for Science and Technology (CONACyT, 284292 ), Fund SEP-Cinvestav ( 108 ), and the Royal Society, UK (Newton Advanced Fellowship, NAF/R1/180017 ).","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa":1,"department":[{"_id":"MiSi"}],"pmid":1,"date_published":"2021-05-24T00:00:00Z","title":"Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion","article_processing_charge":"No","language":[{"iso":"eng"}],"type":"journal_article","_id":"10834","intvolume":"        31","scopus_import":"1","page":"2051-2064.e8","publication_status":"published","isi":1,"citation":{"short":"S. Stahnke, H. Döring, C. Kusch, D.J.J. de Gorter, S. Dütting, A. Guledani, I. Pleines, M. Schnoor, M.K. Sixt, R. Geffers, M. Rohde, M. Müsken, F. Kage, A. Steffen, J. Faix, B. Nieswandt, K. Rottner, T.E.B. Stradal, Current Biology 31 (2021) 2051–2064.e8.","apa":"Stahnke, S., Döring, H., Kusch, C., de Gorter, D. J. J., Dütting, S., Guledani, A., … Stradal, T. E. B. (2021). Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">https://doi.org/10.1016/j.cub.2021.02.043</a>","ama":"Stahnke S, Döring H, Kusch C, et al. Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. <i>Current Biology</i>. 2021;31(10):2051-2064.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">10.1016/j.cub.2021.02.043</a>","chicago":"Stahnke, Stephanie, Hermann Döring, Charly Kusch, David J.J. de Gorter, Sebastian Dütting, Aleks Guledani, Irina Pleines, et al. “Loss of Hem1 Disrupts Macrophage Function and Impacts Migration, Phagocytosis, and Integrin-Mediated Adhesion.” <i>Current Biology</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">https://doi.org/10.1016/j.cub.2021.02.043</a>.","mla":"Stahnke, Stephanie, et al. “Loss of Hem1 Disrupts Macrophage Function and Impacts Migration, Phagocytosis, and Integrin-Mediated Adhesion.” <i>Current Biology</i>, vol. 31, no. 10, Elsevier, 2021, p. 2051–2064.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">10.1016/j.cub.2021.02.043</a>.","ista":"Stahnke S, Döring H, Kusch C, de Gorter DJJ, Dütting S, Guledani A, Pleines I, Schnoor M, Sixt MK, Geffers R, Rohde M, Müsken M, Kage F, Steffen A, Faix J, Nieswandt B, Rottner K, Stradal TEB. 2021. Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. Current Biology. 31(10), 2051–2064.e8.","ieee":"S. Stahnke <i>et al.</i>, “Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion,” <i>Current Biology</i>, vol. 31, no. 10. Elsevier, p. 2051–2064.e8, 2021."},"volume":31,"publisher":"Elsevier","day":"24","author":[{"last_name":"Stahnke","full_name":"Stahnke, Stephanie","first_name":"Stephanie"},{"first_name":"Hermann","full_name":"Döring, Hermann","last_name":"Döring"},{"last_name":"Kusch","full_name":"Kusch, Charly","first_name":"Charly"},{"first_name":"David J.J.","last_name":"de Gorter","full_name":"de Gorter, David J.J."},{"last_name":"Dütting","full_name":"Dütting, Sebastian","first_name":"Sebastian"},{"first_name":"Aleks","full_name":"Guledani, Aleks","last_name":"Guledani"},{"last_name":"Pleines","full_name":"Pleines, Irina","first_name":"Irina"},{"last_name":"Schnoor","full_name":"Schnoor, Michael","first_name":"Michael"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K"},{"first_name":"Robert","last_name":"Geffers","full_name":"Geffers, Robert"},{"full_name":"Rohde, Manfred","last_name":"Rohde","first_name":"Manfred"},{"first_name":"Mathias","full_name":"Müsken, Mathias","last_name":"Müsken"},{"first_name":"Frieda","last_name":"Kage","full_name":"Kage, Frieda"},{"last_name":"Steffen","full_name":"Steffen, Anika","first_name":"Anika"},{"last_name":"Faix","full_name":"Faix, Jan","first_name":"Jan"},{"first_name":"Bernhard","full_name":"Nieswandt, Bernhard","last_name":"Nieswandt"},{"first_name":"Klemens","last_name":"Rottner","full_name":"Rottner, Klemens"},{"last_name":"Stradal","full_name":"Stradal, Theresia E.B.","first_name":"Theresia E.B."}],"doi":"10.1016/j.cub.2021.02.043","quality_controlled":"1","issue":"10","keyword":["General Agricultural and Biological Sciences","General Biochemistry","Genetics and Molecular Biology"],"status":"public","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2020.03.24.005835"}],"oa_version":"Preprint","month":"05","article_type":"original"},{"doi":"10.1016/j.devcel.2021.10.008","quality_controlled":"1","keyword":["Developmental Biology","Cell Biology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology"],"issue":"21","status":"public","oa_version":"None","article_type":"original","month":"11","scopus_import":"1","publication_status":"published","page":"P2952-2965.e9","citation":{"ieee":"S. Krishna, R. Arrojo e Drigo, J. S. Capitanio, R. Ramachandra, M. Ellisman, and M. Hetzer, “Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain,” <i>Developmental Cell</i>, vol. 56, no. 21. Elsevier, p. P2952–2965.e9, 2021.","chicago":"Krishna, Shefali, Rafael Arrojo e Drigo, Juliana S. Capitanio, Ranjan Ramachandra, Mark Ellisman, and Martin Hetzer. “Identification of Long-Lived Proteins in the Mitochondria Reveals Increased Stability of the Electron Transport Chain.” <i>Developmental Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.devcel.2021.10.008\">https://doi.org/10.1016/j.devcel.2021.10.008</a>.","ista":"Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer M. 2021. Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. Developmental Cell. 56(21), P2952–2965.e9.","ama":"Krishna S, Arrojo e Drigo R, Capitanio JS, Ramachandra R, Ellisman M, Hetzer M. Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. <i>Developmental Cell</i>. 2021;56(21):P2952-2965.e9. doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.10.008\">10.1016/j.devcel.2021.10.008</a>","mla":"Krishna, Shefali, et al. “Identification of Long-Lived Proteins in the Mitochondria Reveals Increased Stability of the Electron Transport Chain.” <i>Developmental Cell</i>, vol. 56, no. 21, Elsevier, 2021, p. P2952–2965.e9, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.10.008\">10.1016/j.devcel.2021.10.008</a>.","apa":"Krishna, S., Arrojo e Drigo, R., Capitanio, J. S., Ramachandra, R., Ellisman, M., &#38; Hetzer, M. (2021). Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2021.10.008\">https://doi.org/10.1016/j.devcel.2021.10.008</a>","short":"S. Krishna, R. Arrojo e Drigo, J.S. Capitanio, R. Ramachandra, M. Ellisman, M. Hetzer, Developmental Cell 56 (2021) P2952–2965.e9."},"volume":56,"publisher":"Elsevier","day":"08","author":[{"first_name":"Shefali","full_name":"Krishna, Shefali","last_name":"Krishna"},{"first_name":"Rafael","full_name":"Arrojo e Drigo, Rafael","last_name":"Arrojo e Drigo"},{"last_name":"Capitanio","full_name":"Capitanio, Juliana S.","first_name":"Juliana S."},{"full_name":"Ramachandra, Ranjan","last_name":"Ramachandra","first_name":"Ranjan"},{"full_name":"Ellisman, Mark","last_name":"Ellisman","first_name":"Mark"},{"orcid":"0000-0002-2111-992X","first_name":"Martin W","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","full_name":"HETZER, Martin W","last_name":"HETZER"}],"date_published":"2021-11-08T00:00:00Z","pmid":1,"title":"Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain","article_processing_charge":"No","language":[{"iso":"eng"}],"_id":"11052","type":"journal_article","intvolume":"        56","external_id":{"pmid":["34715012"]},"abstract":[{"lang":"eng","text":"In order to combat molecular damage, most cellular proteins undergo rapid turnover. We have previously identified large nuclear protein assemblies that can persist for years in post-mitotic tissues and are subject to age-related decline. Here, we report that mitochondria can be long lived in the mouse brain and reveal that specific mitochondrial proteins have half-lives longer than the average proteome. These mitochondrial long-lived proteins (mitoLLPs) are core components of the electron transport chain (ETC) and display increased longevity in respiratory supercomplexes. We find that COX7C, a mitoLLP that forms a stable contact site between complexes I and IV, is required for complex IV and supercomplex assembly. Remarkably, even upon depletion of COX7C transcripts, ETC function is maintained for days, effectively uncoupling mitochondrial function from ongoing transcription of its mitoLLPs. Our results suggest that modulating protein longevity within the ETC is critical for mitochondrial proteome maintenance and the robustness of mitochondrial function."}],"date_updated":"2025-12-15T10:01:56Z","year":"2021","publication":"Developmental Cell","date_created":"2022-04-07T07:43:14Z","publication_identifier":{"issn":["1534-5807"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"MaHe"}],"extern":"1"},{"publication_identifier":{"issn":["2041-1723"]},"publication":"Nature Communications","date_created":"2023-02-20T08:11:29Z","date_updated":"2023-02-28T13:21:51Z","year":"2021","abstract":[{"text":"Glaciers in High Mountain Asia generate meltwater that supports the water needs of 250 million people, but current knowledge of annual accumulation and ablation is limited to sparse field measurements biased in location and glacier size. Here, we present altitudinally-resolved specific mass balances (surface, internal, and basal combined) for 5527 glaciers in High Mountain Asia for 2000–2016, derived by correcting observed glacier thinning patterns for mass redistribution due to ice flow. We find that 41% of glaciers accumulated mass over less than 20% of their area, and only 60% ± 10% of regional annual ablation was compensated by accumulation. Even without 21st century warming, 21% ± 1% of ice volume will be lost by 2100 due to current climatic-geometric imbalance, representing a reduction in glacier ablation into rivers of 28% ± 1%. The ablation of glaciers in the Himalayas and Tien Shan was mostly unsustainable and ice volume in these regions will reduce by at least 30% by 2100. The most important and vulnerable glacier-fed river basins (Amu Darya, Indus, Syr Darya, Tarim Interior) were supplied with >50% sustainable glacier ablation but will see long-term reductions in ice mass and glacier meltwater supply regardless of the Karakoram Anomaly.","lang":"eng"}],"extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"language":[{"iso":"eng"}],"title":"Health and sustainability of glaciers in High Mountain Asia","article_processing_charge":"No","date_published":"2021-05-17T00:00:00Z","intvolume":"        12","type":"journal_article","_id":"12585","citation":{"short":"E. Miles, M. McCarthy, A. Dehecq, M. Kneib, S. Fugger, F. Pellicciotti, Nature Communications 12 (2021).","apa":"Miles, E., McCarthy, M., Dehecq, A., Kneib, M., Fugger, S., &#38; Pellicciotti, F. (2021). Health and sustainability of glaciers in High Mountain Asia. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-23073-4\">https://doi.org/10.1038/s41467-021-23073-4</a>","ama":"Miles E, McCarthy M, Dehecq A, Kneib M, Fugger S, Pellicciotti F. Health and sustainability of glaciers in High Mountain Asia. <i>Nature Communications</i>. 2021;12. doi:<a href=\"https://doi.org/10.1038/s41467-021-23073-4\">10.1038/s41467-021-23073-4</a>","mla":"Miles, Evan, et al. “Health and Sustainability of Glaciers in High Mountain Asia.” <i>Nature Communications</i>, vol. 12, 2868, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23073-4\">10.1038/s41467-021-23073-4</a>.","chicago":"Miles, Evan, Michael McCarthy, Amaury Dehecq, Marin Kneib, Stefan Fugger, and Francesca Pellicciotti. “Health and Sustainability of Glaciers in High Mountain Asia.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23073-4\">https://doi.org/10.1038/s41467-021-23073-4</a>.","ista":"Miles E, McCarthy M, Dehecq A, Kneib M, Fugger S, Pellicciotti F. 2021. Health and sustainability of glaciers in High Mountain Asia. Nature Communications. 12, 2868.","ieee":"E. Miles, M. McCarthy, A. Dehecq, M. Kneib, S. Fugger, and F. Pellicciotti, “Health and sustainability of glaciers in High Mountain Asia,” <i>Nature Communications</i>, vol. 12. Springer Nature, 2021."},"publication_status":"published","scopus_import":"1","author":[{"first_name":"Evan","full_name":"Miles, Evan","last_name":"Miles"},{"last_name":"McCarthy","full_name":"McCarthy, Michael","first_name":"Michael"},{"first_name":"Amaury","full_name":"Dehecq, Amaury","last_name":"Dehecq"},{"first_name":"Marin","full_name":"Kneib, Marin","last_name":"Kneib"},{"first_name":"Stefan","full_name":"Fugger, Stefan","last_name":"Fugger"},{"last_name":"Pellicciotti","full_name":"Pellicciotti, Francesca","first_name":"Francesca","id":"b28f055a-81ea-11ed-b70c-a9fe7f7b0e70"}],"day":"17","publisher":"Springer Nature","volume":12,"doi":"10.1038/s41467-021-23073-4","quality_controlled":"1","month":"05","article_type":"original","oa_version":"Published Version","status":"public","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41467-021-23073-4"}],"article_number":"2868","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"]},{"publication_identifier":{"eissn":["1749-6632"],"issn":["0077-8923"]},"date_updated":"2024-10-14T12:12:06Z","year":"2021","publication":"Annals of the New York Academy of Sciences","date_created":"2023-08-01T09:33:39Z","abstract":[{"lang":"eng","text":"Self-assembly of nanoparticles can be mediated by polymers, but has so far led almost exclusively to nanoparticle aggregates that are amorphous. Here, we employed Coulombic interactions to generate a range of composite materials from mixtures of charged nanoparticles and oppositely charged polymers. The assembly behavior of these nanoparticle/polymer composites depends on their order of addition: polymers added to nanoparticles give rise to stable aggregates, but nanoparticles added to polymers disassemble the initially formed aggregates. The amorphous aggregates were transformed into crystalline ones by transiently increasing the ionic strength of the solution. The morphology of the resulting crystals depended on the length of the polymer: short polymer chains mediated the self-assembly of nanoparticles into strongly faceted crystals, whereas long chains led to pseudospherical nanoparticle/polymer assemblies, within which the crystalline order of nanoparticles was retained."}],"external_id":{"pmid":["34427923"]},"extern":"1","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"article_processing_charge":"No","title":"Morphology control in crystalline nanoparticle–polymer aggregates","date_published":"2021-12-01T00:00:00Z","pmid":1,"intvolume":"      1505","type":"journal_article","_id":"13356","citation":{"ieee":"T. Bian and R. Klajn, “Morphology control in crystalline nanoparticle–polymer aggregates,” <i>Annals of the New York Academy of Sciences</i>, vol. 1505, no. 1. Wiley, pp. 191–201, 2021.","short":"T. Bian, R. Klajn, Annals of the New York Academy of Sciences 1505 (2021) 191–201.","apa":"Bian, T., &#38; Klajn, R. (2021). Morphology control in crystalline nanoparticle–polymer aggregates. <i>Annals of the New York Academy of Sciences</i>. Wiley. <a href=\"https://doi.org/10.1111/nyas.14674\">https://doi.org/10.1111/nyas.14674</a>","chicago":"Bian, Tong, and Rafal Klajn. “Morphology Control in Crystalline Nanoparticle–Polymer Aggregates.” <i>Annals of the New York Academy of Sciences</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nyas.14674\">https://doi.org/10.1111/nyas.14674</a>.","ama":"Bian T, Klajn R. Morphology control in crystalline nanoparticle–polymer aggregates. <i>Annals of the New York Academy of Sciences</i>. 2021;1505(1):191-201. doi:<a href=\"https://doi.org/10.1111/nyas.14674\">10.1111/nyas.14674</a>","ista":"Bian T, Klajn R. 2021. Morphology control in crystalline nanoparticle–polymer aggregates. Annals of the New York Academy of Sciences. 1505(1), 191–201.","mla":"Bian, Tong, and Rafal Klajn. “Morphology Control in Crystalline Nanoparticle–Polymer Aggregates.” <i>Annals of the New York Academy of Sciences</i>, vol. 1505, no. 1, Wiley, 2021, pp. 191–201, doi:<a href=\"https://doi.org/10.1111/nyas.14674\">10.1111/nyas.14674</a>."},"publication_status":"published","page":"191-201","scopus_import":"1","author":[{"first_name":"Tong","full_name":"Bian, Tong","last_name":"Bian"},{"id":"8e84690e-1e48-11ed-a02b-a1e6fb8bb53b","first_name":"Rafal","full_name":"Klajn, Rafal","last_name":"Klajn"}],"day":"01","publisher":"Wiley","volume":1505,"quality_controlled":"1","doi":"10.1111/nyas.14674","article_type":"original","month":"12","oa_version":"Published Version","status":"public","main_file_link":[{"url":"https://doi.org/10.1111/nyas.14674","open_access":"1"}],"keyword":["History and Philosophy of Science","General Biochemistry","Genetics and Molecular Biology","General Neuroscience"],"ddc":["540"],"issue":"1"},{"date_published":"2021-08-19T00:00:00Z","pmid":1,"title":"SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation","article_processing_charge":"Yes","language":[{"iso":"eng"}],"_id":"15137","type":"journal_article","intvolume":"        12","external_id":{"pmid":["34413302"]},"abstract":[{"text":"Characteristic properties of type III CRISPR-Cas systems include recognition of target RNA and the subsequent induction of a multifaceted immune response. This involves sequence-specific cleavage of the target RNA and production of cyclic oligoadenylate (cOA) molecules. Here we report that an exposed seed region at the 3′ end of the crRNA is essential for target RNA binding and cleavage, whereas cOA production requires base pairing at the 5′ end of the crRNA. Moreover, we uncover that the variation in the size and composition of type III complexes within a single host results in variable seed regions. This may prevent escape by invading genetic elements, while controlling cOA production tightly to prevent unnecessary damage to the host. Lastly, we use these findings to develop a new diagnostic tool, SCOPE, for the specific detection of SARS-CoV-2 from human nasal swab samples, revealing sensitivities in the atto-molar range.","lang":"eng"}],"date_updated":"2024-06-04T06:11:54Z","year":"2021","date_created":"2024-03-20T10:42:33Z","publication":"Nature Communications","publication_identifier":{"issn":["2041-1723"]},"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","quality_controlled":"1","doi":"10.1038/s41467-021-25337-5","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"article_number":"5033","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41467-021-25337-5"}],"status":"public","oa_version":"Published Version","article_type":"original","month":"08","scopus_import":"1","publication_status":"published","citation":{"apa":"Steens, J. A., Zhu, Y., Taylor, D. W., Bravo, J. P. K., Prinsen, S. H. P., Schoen, C. D., … Staals, R. H. J. (2021). SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-25337-5\">https://doi.org/10.1038/s41467-021-25337-5</a>","short":"J.A. Steens, Y. Zhu, D.W. Taylor, J.P.K. Bravo, S.H.P. Prinsen, C.D. Schoen, B.J.F. Keijser, M. Ossendrijver, L.M. Hofstra, S.J.J. Brouns, A. Shinkai, J. van der Oost, R.H.J. Staals, Nature Communications 12 (2021).","ista":"Steens JA, Zhu Y, Taylor DW, Bravo JPK, Prinsen SHP, Schoen CD, Keijser BJF, Ossendrijver M, Hofstra LM, Brouns SJJ, Shinkai A, van der Oost J, Staals RHJ. 2021. SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation. Nature Communications. 12, 5033.","mla":"Steens, Jurre A., et al. “SCOPE Enables Type III CRISPR-Cas Diagnostics Using Flexible Targeting and Stringent CARF Ribonuclease Activation.” <i>Nature Communications</i>, vol. 12, 5033, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-25337-5\">10.1038/s41467-021-25337-5</a>.","ama":"Steens JA, Zhu Y, Taylor DW, et al. SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation. <i>Nature Communications</i>. 2021;12. doi:<a href=\"https://doi.org/10.1038/s41467-021-25337-5\">10.1038/s41467-021-25337-5</a>","chicago":"Steens, Jurre A., Yifan Zhu, David W. Taylor, Jack Peter Kelly Bravo, Stijn H. P. Prinsen, Cor D. Schoen, Bart J. F. Keijser, et al. “SCOPE Enables Type III CRISPR-Cas Diagnostics Using Flexible Targeting and Stringent CARF Ribonuclease Activation.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-25337-5\">https://doi.org/10.1038/s41467-021-25337-5</a>.","ieee":"J. A. Steens <i>et al.</i>, “SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation,” <i>Nature Communications</i>, vol. 12. Springer Nature, 2021."},"volume":12,"publisher":"Springer Nature","day":"19","author":[{"first_name":"Jurre A.","full_name":"Steens, Jurre A.","last_name":"Steens"},{"first_name":"Yifan","last_name":"Zhu","full_name":"Zhu, Yifan"},{"last_name":"Taylor","full_name":"Taylor, David W.","first_name":"David W."},{"last_name":"Bravo","full_name":"Bravo, Jack Peter Kelly","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","first_name":"Jack Peter Kelly","orcid":"0000-0003-0456-0753"},{"first_name":"Stijn H. P.","last_name":"Prinsen","full_name":"Prinsen, Stijn H. P."},{"last_name":"Schoen","full_name":"Schoen, Cor D.","first_name":"Cor D."},{"first_name":"Bart J. F.","last_name":"Keijser","full_name":"Keijser, Bart J. F."},{"full_name":"Ossendrijver, Michel","last_name":"Ossendrijver","first_name":"Michel"},{"last_name":"Hofstra","full_name":"Hofstra, L. Marije","first_name":"L. Marije"},{"first_name":"Stan J. J.","full_name":"Brouns, Stan J. J.","last_name":"Brouns"},{"full_name":"Shinkai, Akeo","last_name":"Shinkai","first_name":"Akeo"},{"last_name":"van der Oost","full_name":"van der Oost, John","first_name":"John"},{"first_name":"Raymond H. J.","full_name":"Staals, Raymond H. J.","last_name":"Staals"}]},{"issue":"21","keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology","General Neuroscience"],"article_number":"e107711","status":"public","main_file_link":[{"url":"https://doi.org/10.15252/embj.2021107711","open_access":"1"}],"oa_version":"Published Version","month":"11","article_type":"original","quality_controlled":"1","doi":"10.15252/embj.2021107711","volume":40,"publisher":"Embo Press","day":"02","author":[{"first_name":"Florian","last_name":"Geiger","full_name":"Geiger, Florian"},{"full_name":"Acker, Julia","last_name":"Acker","first_name":"Julia"},{"last_name":"Papa","full_name":"Papa, Guido","first_name":"Guido"},{"first_name":"Xinyu","full_name":"Wang, Xinyu","last_name":"Wang"},{"first_name":"William E","full_name":"Arter, William E","last_name":"Arter"},{"first_name":"Kadi L","last_name":"Saar","full_name":"Saar, Kadi L"},{"first_name":"Nadia A","full_name":"Erkamp, Nadia A","last_name":"Erkamp"},{"last_name":"Qi","full_name":"Qi, Runzhang","first_name":"Runzhang"},{"full_name":"Bravo, Jack Peter Kelly","last_name":"Bravo","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","first_name":"Jack Peter Kelly","orcid":"0000-0003-0456-0753"},{"first_name":"Sebastian","full_name":"Strauss, Sebastian","last_name":"Strauss"},{"last_name":"Krainer","full_name":"Krainer, Georg","first_name":"Georg"},{"last_name":"Burrone","full_name":"Burrone, Oscar R","first_name":"Oscar R"},{"last_name":"Jungmann","full_name":"Jungmann, Ralf","first_name":"Ralf"},{"full_name":"Knowles, Tuomas PJ","last_name":"Knowles","first_name":"Tuomas PJ"},{"first_name":"Hanna","last_name":"Engelke","full_name":"Engelke, Hanna"},{"full_name":"Borodavka, Alexander","last_name":"Borodavka","first_name":"Alexander"}],"scopus_import":"1","publication_status":"published","citation":{"ieee":"F. Geiger <i>et al.</i>, “Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses,” <i>The EMBO Journal</i>, vol. 40, no. 21. Embo Press, 2021.","apa":"Geiger, F., Acker, J., Papa, G., Wang, X., Arter, W. E., Saar, K. L., … Borodavka, A. (2021). Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses. <i>The EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2021107711\">https://doi.org/10.15252/embj.2021107711</a>","short":"F. Geiger, J. Acker, G. Papa, X. Wang, W.E. Arter, K.L. Saar, N.A. Erkamp, R. Qi, J.P.K. Bravo, S. Strauss, G. Krainer, O.R. Burrone, R. Jungmann, T.P. Knowles, H. Engelke, A. Borodavka, The EMBO Journal 40 (2021).","mla":"Geiger, Florian, et al. “Liquid–Liquid Phase Separation Underpins the Formation of Replication Factories in Rotaviruses.” <i>The EMBO Journal</i>, vol. 40, no. 21, e107711, Embo Press, 2021, doi:<a href=\"https://doi.org/10.15252/embj.2021107711\">10.15252/embj.2021107711</a>.","ista":"Geiger F, Acker J, Papa G, Wang X, Arter WE, Saar KL, Erkamp NA, Qi R, Bravo JPK, Strauss S, Krainer G, Burrone OR, Jungmann R, Knowles TP, Engelke H, Borodavka A. 2021. Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses. The EMBO Journal. 40(21), e107711.","chicago":"Geiger, Florian, Julia Acker, Guido Papa, Xinyu Wang, William E Arter, Kadi L Saar, Nadia A Erkamp, et al. “Liquid–Liquid Phase Separation Underpins the Formation of Replication Factories in Rotaviruses.” <i>The EMBO Journal</i>. Embo Press, 2021. <a href=\"https://doi.org/10.15252/embj.2021107711\">https://doi.org/10.15252/embj.2021107711</a>.","ama":"Geiger F, Acker J, Papa G, et al. Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses. <i>The EMBO Journal</i>. 2021;40(21). doi:<a href=\"https://doi.org/10.15252/embj.2021107711\">10.15252/embj.2021107711</a>"},"_id":"15138","type":"journal_article","intvolume":"        40","pmid":1,"date_published":"2021-11-02T00:00:00Z","title":"Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses","article_processing_charge":"Yes","language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"extern":"1","external_id":{"pmid":["34524703"]},"abstract":[{"lang":"eng","text":"RNA viruses induce the formation of subcellular organelles that provide microenvironments conducive to their replication. Here we show that replication factories of rotaviruses represent protein‐RNA condensates that are formed via liquid–liquid phase separation of the viroplasm‐forming proteins NSP5 and rotavirus RNA chaperone NSP2. Upon mixing, these proteins readily form condensates at physiologically relevant low micromolar concentrations achieved in the cytoplasm of virus‐infected cells. Early infection stage condensates could be reversibly dissolved by 1,6‐hexanediol, as well as propylene glycol that released rotavirus transcripts from these condensates. During the early stages of infection, propylene glycol treatments reduced viral replication and phosphorylation of the condensate‐forming protein NSP5. During late infection, these condensates exhibited altered material properties and became resistant to propylene glycol, coinciding with hyperphosphorylation of NSP5. Some aspects of the assembly of cytoplasmic rotavirus replication factories mirror the formation of other ribonucleoprotein granules. Such viral RNA‐rich condensates that support replication of multi‐segmented genomes represent an attractive target for developing novel therapeutic approaches."}],"date_created":"2024-03-20T10:42:39Z","publication":"The EMBO Journal","date_updated":"2024-06-04T06:08:16Z","year":"2021","publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]}},{"author":[{"orcid":"0000-0002-6080-839X","id":"6437c950-2a03-11ee-914d-d6476dd7b75c","first_name":"Alicia","last_name":"Michael","full_name":"Michael, Alicia"},{"full_name":"Thomä, Nicolas H.","last_name":"Thomä","first_name":"Nicolas H."}],"day":"08","publisher":"Elsevier","volume":184,"citation":{"ieee":"A. K. Michael and N. H. Thomä, “Reading the chromatinized genome,” <i>Cell</i>, vol. 184, no. 14. Elsevier, pp. 3599–3611, 2021.","ista":"Michael AK, Thomä NH. 2021. Reading the chromatinized genome. Cell. 184(14), 3599–3611.","mla":"Michael, Alicia K., and Nicolas H. Thomä. “Reading the Chromatinized Genome.” <i>Cell</i>, vol. 184, no. 14, Elsevier, 2021, pp. 3599–611, doi:<a href=\"https://doi.org/10.1016/j.cell.2021.05.029\">10.1016/j.cell.2021.05.029</a>.","chicago":"Michael, Alicia K., and Nicolas H. Thomä. “Reading the Chromatinized Genome.” <i>Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.cell.2021.05.029\">https://doi.org/10.1016/j.cell.2021.05.029</a>.","ama":"Michael AK, Thomä NH. Reading the chromatinized genome. <i>Cell</i>. 2021;184(14):3599-3611. doi:<a href=\"https://doi.org/10.1016/j.cell.2021.05.029\">10.1016/j.cell.2021.05.029</a>","apa":"Michael, A. K., &#38; Thomä, N. H. (2021). Reading the chromatinized genome. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2021.05.029\">https://doi.org/10.1016/j.cell.2021.05.029</a>","short":"A.K. Michael, N.H. Thomä, Cell 184 (2021) 3599–3611."},"page":"3599-3611","publication_status":"published","scopus_import":"1","month":"07","article_type":"review","oa_version":"Published Version","status":"public","main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2021.05.029","open_access":"1"}],"issue":"14","keyword":["General Biochemistry","Genetics and Molecular Biology"],"quality_controlled":"1","doi":"10.1016/j.cell.2021.05.029","extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"publication_identifier":{"issn":["0092-8674"]},"publication":"Cell","date_created":"2024-03-21T07:54:19Z","date_updated":"2024-03-25T12:31:39Z","year":"2021","abstract":[{"lang":"eng","text":"Eukaryotic DNA-binding proteins operate in the context of chromatin, where nucleosomes are the elementary building blocks. Nucleosomal DNA is wrapped around a histone core, thereby rendering a large fraction of the DNA surface inaccessible to DNA-binding proteins. Nevertheless, first responders in DNA repair and sequence-specific transcription factors bind DNA target sites obstructed by chromatin. While early studies examined protein binding to histone-free DNA, it is only now beginning to emerge how DNA sequences are interrogated on nucleosomes. These readout strategies range from the release of nucleosomal DNA from histones, to rotational/translation register shifts of the DNA motif, and nucleosome-specific DNA binding modes that differ from those observed on naked DNA. Since DNA motif engagement on nucleosomes strongly depends on position and orientation, we argue that motif location and nucleosome positioning co-determine protein access to DNA in transcription and DNA repair."}],"intvolume":"       184","_id":"15151","type":"journal_article","language":[{"iso":"eng"}],"title":"Reading the chromatinized genome","article_processing_charge":"No","date_published":"2021-07-08T00:00:00Z"},{"file":[{"success":1,"checksum":"bbd4de2e54b7fbc11fba14f59e87fe3f","creator":"dernst","date_updated":"2024-04-09T11:13:07Z","file_id":"15307","date_created":"2024-04-09T11:13:07Z","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_size":6997954,"file_name":"2021_eLife_Balmer.pdf"}],"citation":{"short":"T.S. Balmer, C. Borges Merjane, L.O. Trussell, ELife 10 (2021).","apa":"Balmer, T. S., Borges Merjane, C., &#38; Trussell, L. O. (2021). Incomplete removal of extracellular glutamate controls synaptic transmission and integration at a cerebellar synapse. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.63819\">https://doi.org/10.7554/elife.63819</a>","chicago":"Balmer, Timothy S, Carolina Borges Merjane, and Laurence O Trussell. “Incomplete Removal of Extracellular Glutamate Controls Synaptic Transmission and Integration at a Cerebellar Synapse.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href=\"https://doi.org/10.7554/elife.63819\">https://doi.org/10.7554/elife.63819</a>.","ista":"Balmer TS, Borges Merjane C, Trussell LO. 2021. Incomplete removal of extracellular glutamate controls synaptic transmission and integration at a cerebellar synapse. eLife. 10, e63819.","ama":"Balmer TS, Borges Merjane C, Trussell LO. Incomplete removal of extracellular glutamate controls synaptic transmission and integration at a cerebellar synapse. <i>eLife</i>. 2021;10. doi:<a href=\"https://doi.org/10.7554/elife.63819\">10.7554/elife.63819</a>","mla":"Balmer, Timothy S., et al. “Incomplete Removal of Extracellular Glutamate Controls Synaptic Transmission and Integration at a Cerebellar Synapse.” <i>ELife</i>, vol. 10, e63819, eLife Sciences Publications, 2021, doi:<a href=\"https://doi.org/10.7554/elife.63819\">10.7554/elife.63819</a>.","ieee":"T. S. Balmer, C. Borges Merjane, and L. O. Trussell, “Incomplete removal of extracellular glutamate controls synaptic transmission and integration at a cerebellar synapse,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021."},"publication_status":"published","publisher":"eLife Sciences Publications","volume":10,"author":[{"first_name":"Timothy S","last_name":"Balmer","full_name":"Balmer, Timothy S"},{"full_name":"Borges Merjane, Carolina","last_name":"Borges Merjane","orcid":"0000-0003-0005-401X","id":"4305C450-F248-11E8-B48F-1D18A9856A87","first_name":"Carolina"},{"full_name":"Trussell, Laurence O","last_name":"Trussell","first_name":"Laurence O"}],"day":"22","quality_controlled":"1","doi":"10.7554/elife.63819","status":"public","keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","General Medicine","General Neuroscience"],"article_number":"e63819","ddc":["570"],"article_type":"original","month":"02","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Synapses of glutamatergic mossy fibers (MFs) onto cerebellar unipolar brush cells (UBCs) generate slow excitatory (ON) or inhibitory (OFF) postsynaptic responses dependent on the complement of glutamate receptors expressed on the UBC’s large dendritic brush. Using mouse brain slice recording and computational modeling of synaptic transmission, we found that substantial glutamate is maintained in the UBC synaptic cleft, sufficient to modify spontaneous firing in OFF UBCs and tonically desensitize AMPARs of ON UBCs. The source of this ambient glutamate was spontaneous, spike-independent exocytosis from the MF terminal, and its level was dependent on activity of glutamate transporters EAAT1–2. Increasing levels of ambient glutamate shifted the polarity of evoked synaptic responses in ON UBCs and altered the phase of responses to in vivo-like synaptic activity. Unlike classical fast synapses, receptors at the UBC synapse are virtually always exposed to a significant level of glutamate, which varies in a graded manner during transmission."}],"external_id":{"pmid":["33616036"]},"publication_identifier":{"issn":["2050-084X"]},"date_updated":"2024-04-09T11:15:01Z","year":"2021","date_created":"2024-04-03T07:58:11Z","publication":"eLife","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"PeJo"}],"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"title":"Incomplete removal of extracellular glutamate controls synaptic transmission and integration at a cerebellar synapse","article_processing_charge":"Yes","date_published":"2021-02-22T00:00:00Z","pmid":1,"language":[{"iso":"eng"}],"file_date_updated":"2024-04-09T11:13:07Z","type":"journal_article","_id":"15273","has_accepted_license":"1","intvolume":"        10"},{"external_id":{"isi":["000709050300001"]},"abstract":[{"text":"The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) is a regulatory hub for transcription and RNA processing. Here, we identify PHD-finger protein 3 (PHF3) as a regulator of transcription and mRNA stability that docks onto Pol II CTD through its SPOC domain. We characterize SPOC as a CTD reader domain that preferentially binds two phosphorylated Serine-2 marks in adjacent CTD repeats. PHF3 drives liquid-liquid phase separation of phosphorylated Pol II, colocalizes with Pol II clusters and tracks with Pol II across the length of genes. PHF3 knock-out or SPOC deletion in human cells results in increased Pol II stalling, reduced elongation rate and an increase in mRNA stability, with marked derepression of neuronal genes. Key neuronal genes are aberrantly expressed in Phf3 knock-out mouse embryonic stem cells, resulting in impaired neuronal differentiation. Our data suggest that PHF3 acts as a prominent effector of neuronal gene regulation by bridging transcription with mRNA decay.","lang":"eng"}],"publication":"Nature Communications","date_created":"2021-10-20T14:40:32Z","date_updated":"2024-10-21T06:02:05Z","year":"2021","publication_identifier":{"eissn":["2041-1723"]},"acknowledgement":"D.S. thanks Claudine Kraft, Renée Schroeder, Verena Jantsch, Franz Klein and Peter Schlögelhofer for support. We thank Anita Testa Salmazo for help with purifying Pol II; Matthias Geyer and Robert Düster for sharing DYRK1A kinase; Felix Hartmann and Clemens Plaschka for help with mass photometry; Goran Kokic for design of the arrest assay sequences; Petra van der Lelij for help with generating mESC KO; Maximilian Freilinger for help with the purification of mEGFP-CTD; Stefan Ameres, Nina Fasching and Brian Reichholf for advice on SLAM-seq and for sharing reagents; Laura Gallego Valle for advice regarding LLPS assays; Krzysztof Chylinski for advice regarding CRISPR/Cas9 methodology; VBCF Protein Technologies facility for purifying PHF3 and providing gRNAs and Cas9; VBCF NGS facility for sequencing; Monoclonal antibody facility at the Helmholtz center for Pol II antibodies; Friedrich Propst and Elzbieta Kowalska for advice and for sharing materials; Egon Ogris for sharing materials; Martin Eilers for recommending a ChIP-grade TFIIS antibody; Susanne Opravil, Otto Hudecz, Markus Hartl and Natascha Hartl for mass spectrometry analysis; staff of the X-ray beamlines at the ESRF in Grenoble for their excellent support; Christa Bücker, Anton Meinhart, Clemens Plaschka and members of the Slade lab for critical comments on the manuscript; Life Science Editors for editing assistance. M.B. and D.S. acknowledge support by the FWF-funded DK ‘Chromosome Dynamics’. T.K. is a recipient of the DOC fellowship from the Austrian Academy of Sciences. U.S. is supported by the L’Oreal for Women in Science Austria Fellowship and the Austrian Science Fund (FWF T 795-B30). M.L is supported by the Vienna Science and Technology Fund (WWTF, VRG14-006). R.S. is supported by the Czech Science Foundation (15-17670 S and 21-24460 S), Ministry of Education, Youths and Sports of the Czech Republic (CEITEC 2020 project (LQ1601)), and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement no. 649030); this publication reflects only the author’s view and the Research Executive Agency is not responsible for any use that may be made of the information it contains. M.S. is supported by the Czech Science Foundation (GJ20-21581Y). K.D.C. research is supported by the Austrian Science Fund (FWF) Projects I525 and I1593, P22276, P19060, and W1221, Federal Ministry of Economy, Family and Youth through the initiative ‘Laura Bassi Centres of Expertise’, funding from the Centre of Optimized Structural Studies No. 253275, the Wellcome Trust Collaborative Award (201543/Z/16), COST action BM1405 Non-globular proteins - from sequence to structure, function and application in molecular physiopathology (NGP-NET), the Vienna Science and Technology Fund (WWTF LS17-008), and by the University of Vienna. This project was funded by the MFPL start-up grant, the Vienna Science and Technology Fund (WWTF LS14-001), and the Austrian Science Fund (P31546-B28 and W1258 “DK: Integrative Structural Biology”) to D.S.","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa":1,"department":[{"_id":"CaBe"}],"date_published":"2021-10-19T00:00:00Z","article_processing_charge":"No","title":"PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"language":[{"iso":"eng"}],"file_date_updated":"2021-10-21T13:51:49Z","has_accepted_license":"1","type":"journal_article","_id":"10163","intvolume":"        12","scopus_import":"1","publication_status":"published","citation":{"ieee":"L.-M. Appel <i>et al.</i>, “PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC,” <i>Nature Communications</i>, vol. 12, no. 1. Springer Nature, 2021.","ista":"Appel L-M, Franke V, Bruno M, Grishkovskaya I, Kasiliauskaite A, Kaufmann T, Schoeberl UE, Puchinger MG, Kostrhon S, Ebenwaldner C, Sebesta M, Beltzung E, Mechtler K, Lin G, Vlasova A, Leeb M, Pavri R, Stark A, Akalin A, Stefl R, Bernecky C, Djinovic-Carugo K, Slade D. 2021. PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC. Nature Communications. 12(1), 6078.","chicago":"Appel, Lisa-Marie, Vedran Franke, Melania Bruno, Irina Grishkovskaya, Aiste Kasiliauskaite, Tanja Kaufmann, Ursula E. Schoeberl, et al. “PHF3 Regulates Neuronal Gene Expression through the Pol II CTD Reader Domain SPOC.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-26360-2\">https://doi.org/10.1038/s41467-021-26360-2</a>.","mla":"Appel, Lisa-Marie, et al. “PHF3 Regulates Neuronal Gene Expression through the Pol II CTD Reader Domain SPOC.” <i>Nature Communications</i>, vol. 12, no. 1, 6078, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-26360-2\">10.1038/s41467-021-26360-2</a>.","ama":"Appel L-M, Franke V, Bruno M, et al. PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-26360-2\">10.1038/s41467-021-26360-2</a>","apa":"Appel, L.-M., Franke, V., Bruno, M., Grishkovskaya, I., Kasiliauskaite, A., Kaufmann, T., … Slade, D. (2021). PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-26360-2\">https://doi.org/10.1038/s41467-021-26360-2</a>","short":"L.-M. Appel, V. Franke, M. Bruno, I. Grishkovskaya, A. Kasiliauskaite, T. Kaufmann, U.E. Schoeberl, M.G. Puchinger, S. Kostrhon, C. Ebenwaldner, M. Sebesta, E. Beltzung, K. Mechtler, G. Lin, A. Vlasova, M. Leeb, R. Pavri, A. Stark, A. Akalin, R. Stefl, C. Bernecky, K. Djinovic-Carugo, D. Slade, Nature Communications 12 (2021)."},"file":[{"file_name":"2021_NatComm_Appel.pdf","relation":"main_file","file_size":5111706,"access_level":"open_access","content_type":"application/pdf","date_created":"2021-10-21T13:51:49Z","date_updated":"2021-10-21T13:51:49Z","file_id":"10169","creator":"cchlebak","checksum":"d99fcd51aebde19c21314e3de0148007","success":1}],"isi":1,"volume":12,"publisher":"Springer Nature","day":"19","related_material":{"link":[{"relation":"earlier_version","description":"Preprint ","url":"https://www.biorxiv.org/content/10.1101/2020.02.11.943159"}]},"author":[{"first_name":"Lisa-Marie","full_name":"Appel, Lisa-Marie","last_name":"Appel"},{"full_name":"Franke, Vedran","last_name":"Franke","first_name":"Vedran"},{"last_name":"Bruno","full_name":"Bruno, Melania","first_name":"Melania"},{"full_name":"Grishkovskaya, Irina","last_name":"Grishkovskaya","first_name":"Irina"},{"first_name":"Aiste","full_name":"Kasiliauskaite, Aiste","last_name":"Kasiliauskaite"},{"first_name":"Tanja","full_name":"Kaufmann, Tanja","last_name":"Kaufmann"},{"first_name":"Ursula E.","last_name":"Schoeberl","full_name":"Schoeberl, Ursula E."},{"last_name":"Puchinger","full_name":"Puchinger, Martin G.","first_name":"Martin G."},{"full_name":"Kostrhon, Sebastian","last_name":"Kostrhon","first_name":"Sebastian"},{"first_name":"Carmen","last_name":"Ebenwaldner","full_name":"Ebenwaldner, Carmen"},{"first_name":"Marek","full_name":"Sebesta, Marek","last_name":"Sebesta"},{"first_name":"Etienne","full_name":"Beltzung, Etienne","last_name":"Beltzung"},{"first_name":"Karl","full_name":"Mechtler, Karl","last_name":"Mechtler"},{"last_name":"Lin","full_name":"Lin, Gen","first_name":"Gen"},{"first_name":"Anna","full_name":"Vlasova, Anna","last_name":"Vlasova"},{"first_name":"Martin","full_name":"Leeb, Martin","last_name":"Leeb"},{"first_name":"Rushad","full_name":"Pavri, Rushad","last_name":"Pavri"},{"first_name":"Alexander","full_name":"Stark, Alexander","last_name":"Stark"},{"first_name":"Altuna","full_name":"Akalin, Altuna","last_name":"Akalin"},{"first_name":"Richard","last_name":"Stefl","full_name":"Stefl, Richard"},{"full_name":"Bernecky, Carrie A","last_name":"Bernecky","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","first_name":"Carrie A","orcid":"0000-0003-0893-7036"},{"first_name":"Kristina","full_name":"Djinovic-Carugo, Kristina","last_name":"Djinovic-Carugo"},{"last_name":"Slade","full_name":"Slade, Dea","first_name":"Dea"}],"doi":"10.1038/s41467-021-26360-2","quality_controlled":"1","ddc":["610"],"issue":"1","keyword":["general physics and astronomy","general biochemistry","genetics and molecular biology","general chemistry"],"article_number":"6078","status":"public","oa_version":"Published Version","month":"10","article_type":"original"}]