---
_id: '12119'
abstract:
- lang: eng
  text: Intravascular neutrophils and platelets collaborate in maintaining host integrity,
    but their interaction can also trigger thrombotic complications. We report here
    that cooperation between neutrophil and platelet lineages extends to the earliest
    stages of platelet formation by megakaryocytes in the bone marrow. Using intravital
    microscopy, we show that neutrophils “plucked” intravascular megakaryocyte extensions,
    termed proplatelets, to control platelet production. Following CXCR4-CXCL12-dependent
    migration towards perisinusoidal megakaryocytes, plucking neutrophils actively
    pulled on proplatelets and triggered myosin light chain and extracellular-signal-regulated
    kinase activation through reactive oxygen species. By these mechanisms, neutrophils
    accelerate proplatelet growth and facilitate continuous release of platelets in
    steady state. Following myocardial infarction, plucking neutrophils drove excessive
    release of young, reticulated platelets and boosted the risk of recurrent ischemia.
    Ablation of neutrophil plucking normalized thrombopoiesis and reduced recurrent
    thrombosis after myocardial infarction and thrombus burden in venous thrombosis.
    We establish neutrophil plucking as a target to reduce thromboischemic events.
acknowledgement: "We thank Coung Kieu and Dominik van den Heuvel for excellent technical
  assistance. This work was supported by the German Research Foundation (PE2704/2-1,
  PE2704/3-1 to T.P., SFB 1123-project B06 to S.M., SFB1525 project A07 to D.S, TRR
  332 project A7 to C.S., PO 2247/2-1 to A.P., SFB1116-project B11 to A.P. and B12
  to M.K.), LMU Munich’s Institutional\r\nStrategy LMUexcellent within the framework
  of the German Excellence Initiative (No. 806 32 006 to T.P.), and by the German
  Centre for Cardiovascular Research (DZHK) to T.P. (Postdoc Start-up grant No. 100378833).
  This project has received funding from the European Research Council (ERC) under
  the European Union’s Horizon 2020 research and innovation program (grant agreement
  No. 833440 to S.M.). F.G. received funding from the European Union’s\r\nHorizon
  2020 research and innovation program under the Marie Sk1odowska-Curie grant agreement
  no. 747687. A.H. was funded by RTI2018-095497-B-I00 from Ministerio de Ciencia e
  Innovacio´ n (MICINN), HR17_00527 from Fundacion La Caixa, and Transatlantic Network
  of Excellence (TNE-18CVD04) from the Leducq Foundation. The CNIC is supported by
  the MICINN and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence
  (CEX2020-001041-S). A.P. was supported by the Forschungskommission of the Medical
  Faculty of the Heinrich-Heine-Universität Düsseldorf (No. 18-2019 to A.P.). C.G.
  was supported by the Helmholtz Alliance ‘Aging and Metabolic Programming, AMPro,’
  by the German Federal\r\nMinistry of Education and Research to the German Center
  for Diabetes Research (DZD), and by the Bavarian State Ministry of Health and Care
  through the research project DigiMed Bayern."
article_processing_charge: No
article_type: original
author:
- first_name: Tobias
  full_name: Petzold, Tobias
  last_name: Petzold
- first_name: Zhe
  full_name: Zhang, Zhe
  last_name: Zhang
- first_name: Iván
  full_name: Ballesteros, Iván
  last_name: Ballesteros
- first_name: Inas
  full_name: Saleh, Inas
  last_name: Saleh
- first_name: Amin
  full_name: Polzin, Amin
  last_name: Polzin
- first_name: Manuela
  full_name: Thienel, Manuela
  last_name: Thienel
- first_name: Lulu
  full_name: Liu, Lulu
  last_name: Liu
- first_name: Qurrat
  full_name: Ul Ain, Qurrat
  last_name: Ul Ain
- first_name: Vincent
  full_name: Ehreiser, Vincent
  last_name: Ehreiser
- first_name: Christian
  full_name: Weber, Christian
  last_name: Weber
- first_name: Badr
  full_name: Kilani, Badr
  last_name: Kilani
- first_name: Pontus
  full_name: Mertsch, Pontus
  last_name: Mertsch
- first_name: Jeremias
  full_name: Götschke, Jeremias
  last_name: Götschke
- first_name: Sophie
  full_name: Cremer, Sophie
  last_name: Cremer
- first_name: Wenwen
  full_name: Fu, Wenwen
  last_name: Fu
- first_name: Michael
  full_name: Lorenz, Michael
  last_name: Lorenz
- first_name: Hellen
  full_name: Ishikawa-Ankerhold, Hellen
  last_name: Ishikawa-Ankerhold
- first_name: Elisabeth
  full_name: Raatz, Elisabeth
  last_name: Raatz
- first_name: Shaza
  full_name: El-Nemr, Shaza
  last_name: El-Nemr
- first_name: Agnes
  full_name: Görlach, Agnes
  last_name: Görlach
- first_name: Esther
  full_name: Marhuenda, Esther
  last_name: Marhuenda
- first_name: Konstantin
  full_name: Stark, Konstantin
  last_name: Stark
- first_name: Joachim
  full_name: Pircher, Joachim
  last_name: Pircher
- first_name: David
  full_name: Stegner, David
  last_name: Stegner
- first_name: Christian
  full_name: Gieger, Christian
  last_name: Gieger
- first_name: Marc
  full_name: Schmidt-Supprian, Marc
  last_name: Schmidt-Supprian
- first_name: Florian R
  full_name: Gärtner, Florian R
  id: 397A88EE-F248-11E8-B48F-1D18A9856A87
  last_name: Gärtner
  orcid: 0000-0001-6120-3723
- first_name: Isaac
  full_name: Almendros, Isaac
  last_name: Almendros
- first_name: Malte
  full_name: Kelm, Malte
  last_name: Kelm
- first_name: Christian
  full_name: Schulz, Christian
  last_name: Schulz
- first_name: Andrés
  full_name: Hidalgo, Andrés
  last_name: Hidalgo
- first_name: Steffen
  full_name: Massberg, Steffen
  last_name: Massberg
citation:
  ama: Petzold T, Zhang Z, Ballesteros I, et al. Neutrophil “plucking” on megakaryocytes
    drives platelet production and boosts cardiovascular disease. <i>Immunity</i>.
    2022;55(12):2285-2299.e7. doi:<a href="https://doi.org/10.1016/j.immuni.2022.10.001">10.1016/j.immuni.2022.10.001</a>
  apa: Petzold, T., Zhang, Z., Ballesteros, I., Saleh, I., Polzin, A., Thienel, M.,
    … Massberg, S. (2022). Neutrophil “plucking” on megakaryocytes drives platelet
    production and boosts cardiovascular disease. <i>Immunity</i>. Elsevier. <a href="https://doi.org/10.1016/j.immuni.2022.10.001">https://doi.org/10.1016/j.immuni.2022.10.001</a>
  chicago: Petzold, Tobias, Zhe Zhang, Iván Ballesteros, Inas Saleh, Amin Polzin,
    Manuela Thienel, Lulu Liu, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives
    Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>. Elsevier,
    2022. <a href="https://doi.org/10.1016/j.immuni.2022.10.001">https://doi.org/10.1016/j.immuni.2022.10.001</a>.
  ieee: T. Petzold <i>et al.</i>, “Neutrophil ‘plucking’ on megakaryocytes drives
    platelet production and boosts cardiovascular disease,” <i>Immunity</i>, vol.
    55, no. 12. Elsevier, p. 2285–2299.e7, 2022.
  ista: Petzold T, Zhang Z, Ballesteros I, Saleh I, Polzin A, Thienel M, Liu L, Ul
    Ain Q, Ehreiser V, Weber C, Kilani B, Mertsch P, Götschke J, Cremer S, Fu W, Lorenz
    M, Ishikawa-Ankerhold H, Raatz E, El-Nemr S, Görlach A, Marhuenda E, Stark K,
    Pircher J, Stegner D, Gieger C, Schmidt-Supprian M, Gärtner FR, Almendros I, Kelm
    M, Schulz C, Hidalgo A, Massberg S. 2022. Neutrophil “plucking” on megakaryocytes
    drives platelet production and boosts cardiovascular disease. Immunity. 55(12),
    2285–2299.e7.
  mla: Petzold, Tobias, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet
    Production and Boosts Cardiovascular Disease.” <i>Immunity</i>, vol. 55, no. 12,
    Elsevier, 2022, p. 2285–2299.e7, doi:<a href="https://doi.org/10.1016/j.immuni.2022.10.001">10.1016/j.immuni.2022.10.001</a>.
  short: T. Petzold, Z. Zhang, I. Ballesteros, I. Saleh, A. Polzin, M. Thienel, L.
    Liu, Q. Ul Ain, V. Ehreiser, C. Weber, B. Kilani, P. Mertsch, J. Götschke, S.
    Cremer, W. Fu, M. Lorenz, H. Ishikawa-Ankerhold, E. Raatz, S. El-Nemr, A. Görlach,
    E. Marhuenda, K. Stark, J. Pircher, D. Stegner, C. Gieger, M. Schmidt-Supprian,
    F.R. Gärtner, I. Almendros, M. Kelm, C. Schulz, A. Hidalgo, S. Massberg, Immunity
    55 (2022) 2285–2299.e7.
date_created: 2023-01-12T11:56:54Z
date_published: 2022-12-13T00:00:00Z
date_updated: 2025-04-14T07:43:16Z
day: '13'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1016/j.immuni.2022.10.001
ec_funded: 1
external_id:
  isi:
  - '000922019600003'
  pmid:
  - '36272416'
file:
- access_level: open_access
  checksum: 073267a9c0ad9f85a650053bc7b23777
  content_type: application/pdf
  creator: dernst
  date_created: 2023-01-23T10:18:48Z
  date_updated: 2023-01-23T10:18:48Z
  file_id: '12341'
  file_name: 2022_Immunity_Petzold.pdf
  file_size: 5299475
  relation: main_file
  success: 1
file_date_updated: 2023-01-23T10:18:48Z
has_accepted_license: '1'
intvolume: '        55'
isi: 1
issue: '12'
keyword:
- Infectious Diseases
- Immunology
- Immunology and Allergy
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '12'
oa: 1
oa_version: Published Version
page: 2285-2299.e7
pmid: 1
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '747687'
  name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Immunity
publication_identifier:
  issn:
  - 1074-7613
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Neutrophil “plucking” on megakaryocytes drives platelet production and boosts
  cardiovascular disease
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 55
year: '2022'
...
---
_id: '12252'
abstract:
- lang: eng
  text: The COVID−19 pandemic not only resulted in a global crisis, but also accelerated
    vaccine development and antibody discovery. Herein we report a synthetic humanized
    VHH library development pipeline for nanomolar-range affinity VHH binders to SARS-CoV-2
    variants of concern (VoC) receptor binding domains (RBD) isolation. Trinucleotide-based
    randomization of CDRs by Kunkel mutagenesis with the subsequent rolling-cycle
    amplification resulted in more than 10<jats:sup>11</jats:sup> diverse phage display
    library in a manageable for a single person number of electroporation reactions.
    We identified a number of nanomolar-range affinity VHH binders to SARS-CoV-2 variants
    of concern (VoC) receptor binding domains (RBD) by screening a novel synthetic
    humanized antibody library. In order to explore the most robust and fast method
    for affinity improvement, we performed affinity maturation by CDR1 and CDR2 shuffling
    and avidity engineering by multivalent trimeric VHH fusion protein construction.
    As a result, H7-Fc and G12x3-Fc binders were developed with the affinities in
    nM and pM range respectively. Importantly, these affinities are weakly influenced
    by most of SARS-CoV-2 VoC mutations and they retain moderate binding to BA.4\5.
    The plaque reduction neutralization test (PRNT) resulted in IC50 = 100 ng\ml and
    9.6 ng\ml for H7-Fc and G12x3-Fc antibodies, respectively, for the emerging Omicron
    BA.1 variant. Therefore, these VHH could expand the present landscape of SARS-CoV-2
    neutralization binders with the therapeutic potential for present and future SARS-CoV-2
    variants.
acknowledgement: The authors declare that this study received funding from Immunofusion.
  The funder was not involved in the study design, collection, analysis, interpretation
  of data, the writing of this article or the decision to submit it for publication.
article_number: '965446'
article_processing_charge: No
article_type: original
author:
- first_name: Dmitri
  full_name: Dormeshkin, Dmitri
  last_name: Dormeshkin
- first_name: Michail
  full_name: Shapira, Michail
  last_name: Shapira
- first_name: Simon
  full_name: Dubovik, Simon
  last_name: Dubovik
- first_name: Anton
  full_name: Kavaleuski, Anton
  id: 4968f7ad-eb97-11eb-a6c2-8ed382e8912c
  last_name: Kavaleuski
  orcid: 0000-0003-2091-526X
- first_name: Mikalai
  full_name: Katsin, Mikalai
  last_name: Katsin
- first_name: Alexandr
  full_name: Migas, Alexandr
  last_name: Migas
- first_name: Alexander
  full_name: Meleshko, Alexander
  last_name: Meleshko
- first_name: Sergei
  full_name: Semyonov, Sergei
  last_name: Semyonov
citation:
  ama: Dormeshkin D, Shapira M, Dubovik S, et al. Isolation of an escape-resistant
    SARS-CoV-2 neutralizing nanobody from a novel synthetic nanobody library. <i>Frontiers
    in Immunology</i>. 2022;13. doi:<a href="https://doi.org/10.3389/fimmu.2022.965446">10.3389/fimmu.2022.965446</a>
  apa: Dormeshkin, D., Shapira, M., Dubovik, S., Kavaleuski, A., Katsin, M., Migas,
    A., … Semyonov, S. (2022). Isolation of an escape-resistant SARS-CoV-2 neutralizing
    nanobody from a novel synthetic nanobody library. <i>Frontiers in Immunology</i>.
    Frontiers Media. <a href="https://doi.org/10.3389/fimmu.2022.965446">https://doi.org/10.3389/fimmu.2022.965446</a>
  chicago: Dormeshkin, Dmitri, Michail Shapira, Simon Dubovik, Anton Kavaleuski, Mikalai
    Katsin, Alexandr Migas, Alexander Meleshko, and Sergei Semyonov. “Isolation of
    an Escape-Resistant SARS-CoV-2 Neutralizing Nanobody from a Novel Synthetic Nanobody
    Library.” <i>Frontiers in Immunology</i>. Frontiers Media, 2022. <a href="https://doi.org/10.3389/fimmu.2022.965446">https://doi.org/10.3389/fimmu.2022.965446</a>.
  ieee: D. Dormeshkin <i>et al.</i>, “Isolation of an escape-resistant SARS-CoV-2
    neutralizing nanobody from a novel synthetic nanobody library,” <i>Frontiers in
    Immunology</i>, vol. 13. Frontiers Media, 2022.
  ista: Dormeshkin D, Shapira M, Dubovik S, Kavaleuski A, Katsin M, Migas A, Meleshko
    A, Semyonov S. 2022. Isolation of an escape-resistant SARS-CoV-2 neutralizing
    nanobody from a novel synthetic nanobody library. Frontiers in Immunology. 13,
    965446.
  mla: Dormeshkin, Dmitri, et al. “Isolation of an Escape-Resistant SARS-CoV-2 Neutralizing
    Nanobody from a Novel Synthetic Nanobody Library.” <i>Frontiers in Immunology</i>,
    vol. 13, 965446, Frontiers Media, 2022, doi:<a href="https://doi.org/10.3389/fimmu.2022.965446">10.3389/fimmu.2022.965446</a>.
  short: D. Dormeshkin, M. Shapira, S. Dubovik, A. Kavaleuski, M. Katsin, A. Migas,
    A. Meleshko, S. Semyonov, Frontiers in Immunology 13 (2022).
date_created: 2023-01-16T09:56:57Z
date_published: 2022-09-16T00:00:00Z
date_updated: 2025-06-11T13:42:26Z
day: '16'
ddc:
- '570'
department:
- _id: LeSa
doi: 10.3389/fimmu.2022.965446
external_id:
  isi:
  - '000862479100001'
  pmid:
  - '36189235'
file:
- access_level: open_access
  checksum: f8f5d8110710033d0532e7e08bf9dad4
  content_type: application/pdf
  creator: dernst
  date_created: 2023-01-30T09:22:26Z
  date_updated: 2023-01-30T09:22:26Z
  file_id: '12443'
  file_name: 2022_FrontiersImmunology_Dormeshkin.pdf
  file_size: 5695892
  relation: main_file
  success: 1
file_date_updated: 2023-01-30T09:22:26Z
has_accepted_license: '1'
intvolume: '        13'
isi: 1
keyword:
- Immunology
- Immunology and Allergy
- COVID-19
- SARS-CoV-2
- synthetic library
- RBD
- neutralization nanobody
- VHH
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Immunology
publication_identifier:
  issn:
  - 1664-3224
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Isolation of an escape-resistant SARS-CoV-2 neutralizing nanobody from a novel
  synthetic nanobody library
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2022'
...
---
_id: '10836'
acknowledgement: This  work  was  supported  by  the  Austrian  Science  Fund  (FWF)  grants  MCCA  W1248-B30  and  SFB  F4606-B28  to  EJJ.  CP  received  a  short-term
  research fellowship of the European Federation of Immunological Societies  (EFIS-IL)  for  a  research  visit  at  Biocruces  Bizkaia  Health  Research  Institute,  Barakaldo,  Spain.  VKK  received  an  EFIS-IL  short-term  research  fellowship  for  a  research  visit  at  King’s  College  London.  The
  research was funded by the National Institute for Health Research (NIHR) Biomedical
  Research Centre (BRC) based at Guy's and St Thomas' NHS Foundation Trust and King's
  College London (IS-BRC-1215-20006) (SNK).  The  authors  acknowledge  support  by  the  Medical  Research  Council
  (MR/L023091/1) (SNK); Breast Cancer Now (147; KCL-BCN-Q3)(SNK); Cancer Research
  UK (C30122/A11527; C30122/A15774) (SNK); Cancer  Research  UK  King's  Health  Partners  Centre  at  King's  College  London   (C604/A25135)   (SNK);   CRUK/NIHR   in   England/DoH   for   Scotland,  Wales  and  Northern  Ireland  Experimental  Cancer  Medicine  Centre  (C10355/A15587)  (SNK).  The  views  expressed  are  those  of  the  author(s)  and  not  necessarily  those  of  the  NHS,  the  NIHR  or  the  Department  of  Health.  Additionally,  this  work  was  funded  by  Instituto  de  Salud  Carlos  III  through  the  project  "PI16/01223"  (Co-funded  by  European
  Regional Development Fund; “A way to make Europe”) to FB and  by  the  Department  of  Health,  Basque  Government  through  the  project
  “2019111031” to OZ. OZ is recipient of a Sara Borrell 2017 post-doctoral contract
  “CD17/00128” funded by Instituto de Salud Carlos III (Co-funded by European Social
  Fund; “Investing in your future”).
article_processing_charge: No
article_type: letter_note
author:
- first_name: Christina L.
  full_name: Pranger, Christina L.
  last_name: Pranger
- first_name: Judit
  full_name: Fazekas-Singer, Judit
  id: 36432834-F248-11E8-B48F-1D18A9856A87
  last_name: Fazekas-Singer
  orcid: 0000-0002-8777-3502
- first_name: Verena K.
  full_name: Köhler, Verena K.
  last_name: Köhler
- first_name: Isabella
  full_name: Pali‐Schöll, Isabella
  last_name: Pali‐Schöll
- first_name: Alessandro
  full_name: Fiocchi, Alessandro
  last_name: Fiocchi
- first_name: Sophia N.
  full_name: Karagiannis, Sophia N.
  last_name: Karagiannis
- first_name: Olatz
  full_name: Zenarruzabeitia, Olatz
  last_name: Zenarruzabeitia
- first_name: Francisco
  full_name: Borrego, Francisco
  last_name: Borrego
- first_name: Erika
  full_name: Jensen‐Jarolim, Erika
  last_name: Jensen‐Jarolim
citation:
  ama: 'Pranger CL, Singer J, Köhler VK, et al. PIPE‐cloned human IgE and IgG4 antibodies:
    New tools for investigating cow’s milk allergy and tolerance. <i>Allergy</i>.
    2021;76(5):1553-1556. doi:<a href="https://doi.org/10.1111/all.14604">10.1111/all.14604</a>'
  apa: 'Pranger, C. L., Singer, J., Köhler, V. K., Pali‐Schöll, I., Fiocchi, A., Karagiannis,
    S. N., … Jensen‐Jarolim, E. (2021). PIPE‐cloned human IgE and IgG4 antibodies:
    New tools for investigating cow’s milk allergy and tolerance. <i>Allergy</i>.
    Wiley. <a href="https://doi.org/10.1111/all.14604">https://doi.org/10.1111/all.14604</a>'
  chicago: 'Pranger, Christina L., Judit Singer, Verena K. Köhler, Isabella Pali‐Schöll,
    Alessandro Fiocchi, Sophia N. Karagiannis, Olatz Zenarruzabeitia, Francisco Borrego,
    and Erika Jensen‐Jarolim. “PIPE‐cloned Human IgE and IgG4 Antibodies: New Tools
    for Investigating Cow’s Milk Allergy and Tolerance.” <i>Allergy</i>. Wiley, 2021.
    <a href="https://doi.org/10.1111/all.14604">https://doi.org/10.1111/all.14604</a>.'
  ieee: 'C. L. Pranger <i>et al.</i>, “PIPE‐cloned human IgE and IgG4 antibodies:
    New tools for investigating cow’s milk allergy and tolerance,” <i>Allergy</i>,
    vol. 76, no. 5. Wiley, pp. 1553–1556, 2021.'
  ista: 'Pranger CL, Singer J, Köhler VK, Pali‐Schöll I, Fiocchi A, Karagiannis SN,
    Zenarruzabeitia O, Borrego F, Jensen‐Jarolim E. 2021. PIPE‐cloned human IgE and
    IgG4 antibodies: New tools for investigating cow’s milk allergy and tolerance.
    Allergy. 76(5), 1553–1556.'
  mla: 'Pranger, Christina L., et al. “PIPE‐cloned Human IgE and IgG4 Antibodies:
    New Tools for Investigating Cow’s Milk Allergy and Tolerance.” <i>Allergy</i>,
    vol. 76, no. 5, Wiley, 2021, pp. 1553–56, doi:<a href="https://doi.org/10.1111/all.14604">10.1111/all.14604</a>.'
  short: C.L. Pranger, J. Singer, V.K. Köhler, I. Pali‐Schöll, A. Fiocchi, S.N. Karagiannis,
    O. Zenarruzabeitia, F. Borrego, E. Jensen‐Jarolim, Allergy 76 (2021) 1553–1556.
date_created: 2022-03-08T11:19:05Z
date_published: 2021-05-01T00:00:00Z
date_updated: 2023-09-05T15:58:53Z
day: '01'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1111/all.14604
external_id:
  isi:
  - '000577708800001'
  pmid:
  - '32990982'
file:
- access_level: open_access
  checksum: 9526f9554112fc027c9f7fa540c488cd
  content_type: application/pdf
  creator: dernst
  date_created: 2022-03-08T11:23:16Z
  date_updated: 2022-03-08T11:23:16Z
  file_id: '10837'
  file_name: 2021_Allergy_Pranger.pdf
  file_size: 626081
  relation: main_file
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intvolume: '        76'
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keyword:
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page: 1553-1556
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publication: Allergy
publication_identifier:
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title: 'PIPE‐cloned human IgE and IgG4 antibodies: New tools for investigating cow''s
  milk allergy and tolerance'
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type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 76
year: '2021'
...