@inbook{22925,
  abstract     = {Hybridization Chain Reaction (HCR) enables highly sensitive and multiplexed detection of mRNA with subcellular spatial resolution. It employs fluorophore-tagged DNA hairpins that self-assemble on target-bound probe pairs, amplifying the signal without enzymatic reactions. Here, we describe a protocol for performing HCR fluorescence in situ hybridization (FISH) on Xenopus tissue sections. We also outline a semi-automated image analysis pipeline that enables per-cell quantification of probe expression and co-localization. This method provides a robust and quantitative approach for visualizing gene expression patterns in situ.},
  author       = {Vijatovic, David and Papadopoulos, Stavros and Dalla Vecchia, Marco and Sweeney, Lora Beatrice Jaeger},
  booktitle    = {Xenopus},
  editor       = {Beck, Caroline W.},
  issn         = {1940-6029},
  keywords     = {mRNA FISH, Hybridization chain reaction, In situ hybridization, Fluorescence microscopy},
  pages        = {245--259},
  publisher    = {Springer},
  title        = {{Fluorescent in situ mRNA hybridization (FISH) using Hybridization Chain Reaction (HCR) in Xenopus cryosections}},
  doi          = {10.1007/978-1-0716-5360-9_11},
  volume       = {3049},
  year         = {2026},
}