[{"publication_identifier":{"issn":["2663-337X"]},"OA_place":"publisher","_id":"9962","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_created":"2021-08-29T12:36:50Z","department":[{"_id":"GradSch"},{"_id":"SiHi"}],"title":"Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"has_accepted_license":"1","citation":{"apa":"Hansen, A. H. (2021). <i>Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:9962\">https://doi.org/10.15479/at:ista:9962</a>","ama":"Hansen AH. Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration. 2021. doi:<a href=\"https://doi.org/10.15479/at:ista:9962\">10.15479/at:ista:9962</a>","short":"A.H. Hansen, Cell-Autonomous Gene Function and Non-Cell-Autonomous Effects in Radial Projection Neuron Migration, Institute of Science and Technology Austria, 2021.","ista":"Hansen AH. 2021. Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration. Institute of Science and Technology Austria.","mla":"Hansen, Andi H. <i>Cell-Autonomous Gene Function and Non-Cell-Autonomous Effects in Radial Projection Neuron Migration</i>. Institute of Science and Technology Austria, 2021, doi:<a href=\"https://doi.org/10.15479/at:ista:9962\">10.15479/at:ista:9962</a>.","ieee":"A. H. Hansen, “Cell-autonomous gene function and non-cell-autonomous effects in radial projection neuron migration,” Institute of Science and Technology Austria, 2021.","chicago":"Hansen, Andi H. “Cell-Autonomous Gene Function and Non-Cell-Autonomous Effects in Radial Projection Neuron Migration.” Institute of Science and Technology Austria, 2021. <a href=\"https://doi.org/10.15479/at:ista:9962\">https://doi.org/10.15479/at:ista:9962</a>."},"ddc":["570"],"publication_status":"published","oa":1,"alternative_title":["ISTA Thesis"],"license":"https://creativecommons.org/licenses/by/4.0/","month":"09","keyword":["Neuronal migration","Non-cell-autonomous","Cell-autonomous","Neurodevelopmental disease"],"related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"8569"},{"status":"public","relation":"part_of_dissertation","id":"960"}]},"year":"2021","project":[{"_id":"2625A13E-B435-11E9-9278-68D0E5697425","grant_number":"24812","name":"Molecular mechanisms of radial neuronal migration"}],"day":"02","date_updated":"2026-04-08T07:19:09Z","degree_awarded":"PhD","type":"dissertation","supervisor":[{"full_name":"Hippenmeyer, Simon","last_name":"Hippenmeyer","orcid":"0000-0003-2279-1061","first_name":"Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87"}],"author":[{"first_name":"Andi H","id":"38853E16-F248-11E8-B48F-1D18A9856A87","last_name":"Hansen","full_name":"Hansen, Andi H"}],"publisher":"Institute of Science and Technology Austria","status":"public","abstract":[{"lang":"eng","text":"The brain is one of the largest and most complex organs and it is composed of billions of neurons that communicate together enabling e.g. consciousness. The cerebral cortex is the largest site of neural integration in the central nervous system. Concerted radial migration of newly born cortical projection neurons, from their birthplace to their final position, is a key step in the assembly of the cerebral cortex. The cellular and molecular mechanisms regulating radial neuronal migration in vivo are however still unclear. Recent evidence suggests that distinct signaling cues act cell-autonomously but differentially at certain steps during the overall migration process. Moreover, functional analysis of genetic mosaics (mutant neurons present in wild-type/heterozygote environment) using the MADM (Mosaic Analysis with Double Markers) analyses in comparison to global knockout also indicate a significant degree of non-cell-autonomous and/or community effects in the control of cortical neuron migration. The interactions of cell-intrinsic (cell-autonomous) and cell-extrinsic (non-cell-autonomous) components are largely unknown. In part of this thesis work we established a MADM-based experimental strategy for the quantitative analysis of cell-autonomous gene function versus non-cell-autonomous and/or community effects. The direct comparison of mutant neurons from the genetic mosaic (cell-autonomous) to mutant neurons in the conditional and/or global knockout (cell-autonomous + non-cell-autonomous) allows to quantitatively analyze non-cell-autonomous effects. Such analysis enable the high-resolution analysis of projection neuron migration dynamics in distinct environments with concomitant isolation of genomic and proteomic profiles. Using these experimental paradigms and in combination with computational modeling we show and characterize the nature of non-cell-autonomous effects to coordinate radial neuron migration. Furthermore, this thesis discusses recent developments in neurodevelopment with focus on neuronal polarization and non-cell-autonomous mechanisms in neuronal migration."}],"corr_author":"1","file":[{"file_size":10629190,"creator":"ahansen","access_level":"closed","date_updated":"2022-09-03T22:30:04Z","file_id":"9971","file_name":"Thesis_Hansen.docx","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","embargo_to":"open_access","checksum":"66b56f5b988b233dc66a4f4b4fb2cdfe","date_created":"2021-08-30T09:17:39Z","relation":"source_file"},{"relation":"main_file","checksum":"204fa40321a1c6289b68c473634c4bf3","date_created":"2021-08-30T09:29:44Z","embargo":"2022-09-02","content_type":"application/pdf","date_updated":"2022-09-03T22:30:04Z","file_id":"9972","file_name":"Thesis_Hansen_PDFA-1a.pdf","access_level":"open_access","creator":"ahansen","file_size":13457469}],"oa_version":"Published Version","doi":"10.15479/at:ista:9962","article_processing_charge":"No","page":"182","file_date_updated":"2022-09-03T22:30:04Z","language":[{"iso":"eng"}],"date_published":"2021-09-02T00:00:00Z"},{"year":"2011","external_id":{"isi":["000296060100011"]},"keyword":["Epithelial cohesion","Hindbrain","Neuronal migration","Zebrafish"],"intvolume":"       138","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"month":"11","scopus_import":"1","article_type":"original","oa":1,"publication_status":"published","ddc":["570"],"acknowledgement":"We thank C. Moens and J. Geiger for critical reading of earlier versions of this manuscript and members of the Heisenberg laboratory for discussions. We are grateful to the microscopy facility of the MPI-CBG and IST Austria for continuous support; I. Nüsslein, J. Compagnon and Alex Eichner for help with cell sorting; and the fish facility of the MPI-CBG and IST Austria for excellent fish care.","has_accepted_license":"1","citation":{"ista":"Stockinger P, Heisenberg C-PJ, Maître J-L. 2011. Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. Development. 138(21), 4673–4683.","short":"P. Stockinger, C.-P.J. Heisenberg, J.-L. Maître, Development 138 (2011) 4673–4683.","apa":"Stockinger, P., Heisenberg, C.-P. J., &#38; Maître, J.-L. (2011). Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.071233\">https://doi.org/10.1242/dev.071233</a>","ama":"Stockinger P, Heisenberg C-PJ, Maître J-L. Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. <i>Development</i>. 2011;138(21):4673-4683. doi:<a href=\"https://doi.org/10.1242/dev.071233\">10.1242/dev.071233</a>","chicago":"Stockinger, Petra, Carl-Philipp J Heisenberg, and Jean-Léon Maître. “Defective Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” <i>Development</i>. Company of Biologists, 2011. <a href=\"https://doi.org/10.1242/dev.071233\">https://doi.org/10.1242/dev.071233</a>.","ieee":"P. Stockinger, C.-P. J. Heisenberg, and J.-L. Maître, “Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube,” <i>Development</i>, vol. 138, no. 21. Company of Biologists, pp. 4673–4683, 2011.","mla":"Stockinger, Petra, et al. “Defective Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” <i>Development</i>, vol. 138, no. 21, Company of Biologists, 2011, pp. 4673–83, doi:<a href=\"https://doi.org/10.1242/dev.071233\">10.1242/dev.071233</a>."},"quality_controlled":"1","department":[{"_id":"CaHe"}],"title":"Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube","date_created":"2018-12-11T12:03:06Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"3396","publist_id":"3210","file_date_updated":"2020-07-14T12:46:12Z","language":[{"iso":"eng"}],"date_published":"2011-11-01T00:00:00Z","volume":138,"doi":"10.1242/dev.071233","article_processing_charge":"No","page":"4673 - 4683","oa_version":"Published Version","file":[{"content_type":"application/pdf","file_id":"6930","file_name":"2011_Development_Stockinger.pdf","date_updated":"2020-07-14T12:46:12Z","access_level":"open_access","creator":"dernst","file_size":4672439,"relation":"main_file","checksum":"ca12b79e01ef36c1ef1aea31cf7e7139","date_created":"2019-10-07T14:19:42Z"}],"publication":"Development","issue":"21","status":"public","abstract":[{"lang":"eng","text":"Facial branchiomotor neurons (FBMNs) in zebrafish and mouse embryonic hindbrain undergo a characteristic tangential migration from rhombomere (r) 4, where they are born, to r6/7. Cohesion among neuroepithelial cells (NCs) has been suggested to function in FBMN migration by inhibiting FBMNs positioned in the basal neuroepithelium such that they move apically between NCs towards the midline of the neuroepithelium instead of tangentially along the basal side of the neuroepithelium towards r6/7. However, direct experimental evaluation of this hypothesis is still lacking. Here, we have used a combination of biophysical cell adhesion measurements and high-resolution time-lapse microscopy to determine the role of NC cohesion in FBMN migration. We show that reducing NC cohesion by interfering with Cadherin 2 (Cdh2) activity results in FBMNs positioned at the basal side of the neuroepithelium moving apically towards the neural tube midline instead of tangentially towards r6/7. In embryos with strongly reduced NC cohesion, ectopic apical FBMN movement frequently results in fusion of the bilateral FBMN clusters over the apical midline of the neural tube. By contrast, reducing cohesion among FBMNs by interfering with Contactin 2 (Cntn2) expression in these cells has little effect on apical FBMN movement, but reduces the fusion of the bilateral FBMN clusters in embryos with strongly diminished NC cohesion. These data provide direct experimental evidence that NC cohesion functions in tangential FBMN migration by restricting their apical movement."}],"corr_author":"1","publisher":"Company of Biologists","author":[{"full_name":"Stockinger, Petra","last_name":"Stockinger","id":"261CB030-E90D-11E9-B182-F697D44B663C","first_name":"Petra"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"},{"orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","first_name":"Jean-Léon","last_name":"Maître","full_name":"Maître, Jean-Léon"}],"type":"journal_article","date_updated":"2026-07-28T08:23:30Z","day":"01","isi":1}]