[{"project":[{"name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"747687"}],"fulldoi":"https://doi.org/10.21769/bioprotoc.3018","year":"2018","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"18","article_type":"original","type":"journal_article","month":"09","DOAJ_listed":"1","oa":1,"article_processing_charge":"Yes","external_id":{"pmid":["34395806"]},"publication":"Bio-Protocol","OA_place":"publisher","acknowledgement":"This protocol was adapted from a previously published study (Gaertner et al., 2017). We thank Michael Lorenz for his excellent assistance in bacteria culture. This work was funded by the DFG SFB 914 (S.M. [B02 and Z01]), the DFG SFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and F.G.), the German Centre for Cardiovascular Research (DZHK) (MHA 1.4VD [S.M.]), FP7 program (project 260309, PRESTIGE [S.M.]), FöFoLe project 947 (F.G.), the Friedrich-Baur-Stiftung project 41/16 (F.G.), Marie Sklodowska Curie Individual Fellowship (EU project 747687, LamelliaActin [F.G.]).","has_accepted_license":"1","ddc":["570"],"publisher":"Bio-Protocol","corr_author":"1","publication_identifier":{"issn":["2331-8325"]},"abstract":[{"lang":"eng","text":"Blood platelets are critical for hemostasis and thrombosis, but also play diverse roles during immune responses. We have recently reported that platelets migrate at sites of infection in vitro and in vivo. Importantly, platelets use their ability to migrate to collect and bundle fibrin (ogen)-bound bacteria accomplishing efficient intravascular bacterial trapping. Here, we describe a method that allows analyzing platelet migration in vitro, focusing on their ability to collect bacteria and trap bacteria under flow."}],"day":"20","volume":8,"OA_type":"gold","pmid":1,"publication_status":"published","date_published":"2018-09-20T00:00:00Z","author":[{"full_name":"Fan, Shuxia","first_name":"Shuxia","last_name":"Fan"},{"full_name":"Lorenz, Michael","first_name":"Michael","last_name":"Lorenz"},{"last_name":"Massberg","first_name":"Steffen","full_name":"Massberg, Steffen"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner","orcid":"0000-0001-6120-3723","first_name":"Florian R","full_name":"Gärtner, Florian R"}],"file":[{"file_id":"6360","access_level":"open_access","file_name":"2018_BioProtocol_Fan.pdf","creator":"dernst","date_created":"2019-04-30T08:04:33Z","relation":"main_file","date_updated":"2020-07-14T12:47:28Z","checksum":"d4588377e789da7f360b553ae02c5119","file_size":2928337,"content_type":"application/pdf"}],"keyword":["Platelets","Cell migration","Bacteria","Shear flow","Fibrinogen","E. coli"],"title":"Platelet migration and bacterial trapping assay under flow","file_date_updated":"2020-07-14T12:47:28Z","doi":"10.21769/bioprotoc.3018","ec_funded":1,"oa_version":"Published Version","language":[{"iso":"eng"}],"department":[{"_id":"MiSi"}],"citation":{"ieee":"S. Fan, M. Lorenz, S. Massberg, and F. R. Gärtner, “Platelet migration and bacterial trapping assay under flow,” <i>Bio-Protocol</i>, vol. 8, no. 18. Bio-Protocol, 2018.","ama":"Fan S, Lorenz M, Massberg S, Gärtner FR. Platelet migration and bacterial trapping assay under flow. <i>Bio-Protocol</i>. 2018;8(18). doi:<a href=\"https://doi.org/10.21769/bioprotoc.3018\">10.21769/bioprotoc.3018</a>","ista":"Fan S, Lorenz M, Massberg S, Gärtner FR. 2018. Platelet migration and bacterial trapping assay under flow. Bio-Protocol. 8(18), e3018.","mla":"Fan, Shuxia, et al. “Platelet Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>, vol. 8, no. 18, e3018, Bio-Protocol, 2018, doi:<a href=\"https://doi.org/10.21769/bioprotoc.3018\">10.21769/bioprotoc.3018</a>.","short":"S. Fan, M. Lorenz, S. Massberg, F.R. Gärtner, Bio-Protocol 8 (2018).","chicago":"Fan, Shuxia, Michael Lorenz, Steffen Massberg, and Florian R Gärtner. “Platelet Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>. Bio-Protocol, 2018. <a href=\"https://doi.org/10.21769/bioprotoc.3018\">https://doi.org/10.21769/bioprotoc.3018</a>.","apa":"Fan, S., Lorenz, M., Massberg, S., &#38; Gärtner, F. R. (2018). Platelet migration and bacterial trapping assay under flow. <i>Bio-Protocol</i>. Bio-Protocol. <a href=\"https://doi.org/10.21769/bioprotoc.3018\">https://doi.org/10.21769/bioprotoc.3018</a>"},"status":"public","intvolume":"         8","date_created":"2019-04-29T09:40:33Z","quality_controlled":"1","article_number":"e3018","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"date_updated":"2025-05-20T07:43:06Z","_id":"6354"}]