---
_id: '17042'
abstract:
- lang: eng
  text: 'Bacterial cell walls are gigadalton-large cross-linked polymers with a wide
    range of motional amplitudes, including rather rigid as well as highly flexible
    parts. Magic-angle spinning NMR is a powerful method to obtain atomic-level information
    about intact cell walls. Here we investigate sensitivity and information content
    of different homonuclear 13C-13C and heteronuclear H-N, H-C and N-C correlation
    experiments. We demonstrate that a CPMAS CryoProbe yields ca. 8-fold increased
    signal-to-noise over a room-temperature probe, or a ca. 3-4-fold larger per-mass
    sensitivity. The increased sensitivity allowed to obtain high-resolution spectra
    even on intact bacteria. Moreover, we compare resolution and sensitivity of 1H
    MAS experiments obtained at 100 kHz vs. 55 kHz. Our study provides useful hints
    for choosing experiments to extract atomic-level details on cell-wall samples. '
article_processing_charge: No
author:
- first_name: Paul
  full_name: Schanda, Paul
  id: 7B541462-FAF6-11E9-A490-E8DFE5697425
  last_name: Schanda
  orcid: 0000-0002-9350-7606
citation:
  ama: 'Schanda P. Raw data to “MAS NMR experiments of corynebacterial cell walls:
    complementary 1H- and CPMAS CryoProbe-enhanced 13C-detected experiments.” 2024.
    doi:<a href="https://doi.org/10.15479/AT:ISTA:17042">10.15479/AT:ISTA:17042</a>'
  apa: 'Schanda, P. (2024). Raw data to “MAS NMR experiments of corynebacterial cell
    walls: complementary 1H- and CPMAS CryoProbe-enhanced 13C-detected experiments.”
    Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:17042">https://doi.org/10.15479/AT:ISTA:17042</a>'
  chicago: 'Schanda, Paul. “Raw Data to ‘MAS NMR Experiments of Corynebacterial Cell
    Walls: Complementary 1H- and CPMAS CryoProbe-Enhanced 13C-Detected Experiments.’”
    Institute of Science and Technology Austria, 2024. <a href="https://doi.org/10.15479/AT:ISTA:17042">https://doi.org/10.15479/AT:ISTA:17042</a>.'
  ieee: 'P. Schanda, “Raw data to ‘MAS NMR experiments of corynebacterial cell walls:
    complementary 1H- and CPMAS CryoProbe-enhanced 13C-detected experiments.’” Institute
    of Science and Technology Austria, 2024.'
  ista: 'Schanda P. 2024. Raw data to ‘MAS NMR experiments of corynebacterial cell
    walls: complementary 1H- and CPMAS CryoProbe-enhanced 13C-detected experiments’,
    Institute of Science and Technology Austria, <a href="https://doi.org/10.15479/AT:ISTA:17042">10.15479/AT:ISTA:17042</a>.'
  mla: 'Schanda, Paul. <i>Raw Data to “MAS NMR Experiments of Corynebacterial Cell
    Walls: Complementary 1H- and CPMAS CryoProbe-Enhanced 13C-Detected Experiments.”</i>
    Institute of Science and Technology Austria, 2024, doi:<a href="https://doi.org/10.15479/AT:ISTA:17042">10.15479/AT:ISTA:17042</a>.'
  short: P. Schanda, (2024).
contributor:
- contributor_type: data_collector
  first_name: Alicia
  last_name: Vallet
- contributor_type: data_collector
  first_name: 'Isabel '
  last_name: Ayala
- contributor_type: data_collector
  first_name: Barbara
  last_name: Perrone
- contributor_type: data_collector
  first_name: Alia
  last_name: Hassan
- contributor_type: data_collector
  first_name: Catherine
  last_name: Bougault
corr_author: '1'
date_created: 2024-05-22T12:04:54Z
date_published: 2024-05-22T00:00:00Z
date_updated: 2025-09-09T12:01:41Z
day: '22'
ddc:
- '570'
department:
- _id: PaSc
doi: 10.15479/AT:ISTA:17042
file:
- access_level: open_access
  checksum: eb55f0988342d927702353b75e07edfa
  content_type: text/plain
  creator: pschanda
  date_created: 2024-05-22T12:05:13Z
  date_updated: 2024-05-22T12:05:13Z
  file_id: '17043'
  file_name: Read_me.txt
  file_size: 2132
  relation: main_file
  success: 1
- access_level: open_access
  checksum: 3393592acaf5ee1e032052c236780914
  content_type: application/zip
  creator: pschanda
  date_created: 2024-05-22T12:17:10Z
  date_updated: 2024-05-22T12:17:10Z
  file_id: '17044'
  file_name: raw_data_CryoMAS_cyronebacteria.zip
  file_size: 755704888
  relation: main_file
  success: 1
file_date_updated: 2024-05-22T12:17:10Z
has_accepted_license: '1'
keyword:
- nuclear magnetic resonance
- NMR
- cellwall
- structural biology
- spectroscopy
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '05'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '17291'
    relation: used_in_publication
    status: public
status: public
title: 'Raw data to "MAS NMR experiments of corynebacterial cell walls: complementary
  1H- and CPMAS CryoProbe-enhanced 13C-detected experiments"'
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...