[{"year":"2021","article_type":"original","external_id":{"pmid":["34448451"],"isi":["000700428500001"]},"pmid":1,"language":[{"iso":"eng"}],"file":[{"access_level":"open_access","content_type":"application/pdf","creator":"dernst","success":1,"date_created":"2022-05-13T08:03:37Z","date_updated":"2022-05-13T08:03:37Z","file_size":9010446,"checksum":"a3f82b0499cc822ac1eab48a01f3f57e","file_name":"2021_eLife_Pulgar.pdf","relation":"main_file","file_id":"11371"}],"isi":1,"publication_identifier":{"eissn":["2050-084X"]},"publication":"eLife","publication_status":"published","title":"Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism","abstract":[{"lang":"eng","text":"The developmental strategies used by progenitor cells to endure a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. Here we uncovered a progenitor cell allocation mechanism that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. Progenitors of the zebrafish laterality organ originate from the surface epithelial enveloping layer by an apical constriction process of cell delamination. During this process, progenitors retain long-term apical contacts that enable the epithelial layer to pull a subset of progenitors along their way towards the vegetal pole. The remaining delaminated progenitors follow apically-attached progenitors’ movement by a co-attraction mechanism, avoiding sequestration by the adjacent endoderm, ensuring their fate and collective allocation at the differentiation site. Thus, we reveal that incomplete delamination serves as a cellular platform for coordinated tissue movements during development. Impact Statement: Incomplete delamination serves as a cellular platform for coordinated tissue movements during development, guiding newly formed progenitor cell groups to the differentiation site."}],"author":[{"last_name":"Pulgar","first_name":"Eduardo","full_name":"Pulgar, Eduardo"},{"first_name":"Cornelia","full_name":"Schwayer, Cornelia","last_name":"Schwayer","id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226"},{"last_name":"Guerrero","first_name":"Néstor","full_name":"Guerrero, Néstor"},{"first_name":"Loreto","full_name":"López, Loreto","last_name":"López"},{"last_name":"Márquez","first_name":"Susana","full_name":"Márquez, Susana"},{"full_name":"Härtel, Steffen","first_name":"Steffen","last_name":"Härtel"},{"last_name":"Soto","first_name":"Rodrigo","full_name":"Soto, Rodrigo"},{"full_name":"Heisenberg, Carl Philipp","first_name":"Carl Philipp","last_name":"Heisenberg"},{"last_name":"Concha","first_name":"Miguel L.","full_name":"Concha, Miguel L."}],"ddc":["570"],"day":"27","article_processing_charge":"Yes","oa":1,"article_number":"e66483","status":"public","ec_funded":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":"        10","citation":{"ista":"Pulgar E, Schwayer C, Guerrero N, López L, Márquez S, Härtel S, Soto R, Heisenberg CP, Concha ML. 2021. Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. eLife. 10, e66483.","mla":"Pulgar, Eduardo, et al. “Apical Contacts Stemming from Incomplete Delamination Guide Progenitor Cell Allocation through a Dragging Mechanism.” <i>ELife</i>, vol. 10, e66483, eLife Sciences Publications, 2021, doi:<a href=\"https://doi.org/10.7554/eLife.66483\">10.7554/eLife.66483</a>.","chicago":"Pulgar, Eduardo, Cornelia Schwayer, Néstor Guerrero, Loreto López, Susana Márquez, Steffen Härtel, Rodrigo Soto, Carl Philipp Heisenberg, and Miguel L. Concha. “Apical Contacts Stemming from Incomplete Delamination Guide Progenitor Cell Allocation through a Dragging Mechanism.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href=\"https://doi.org/10.7554/eLife.66483\">https://doi.org/10.7554/eLife.66483</a>.","short":"E. Pulgar, C. Schwayer, N. Guerrero, L. López, S. Márquez, S. Härtel, R. Soto, C.P. Heisenberg, M.L. Concha, ELife 10 (2021).","ama":"Pulgar E, Schwayer C, Guerrero N, et al. Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. <i>eLife</i>. 2021;10. doi:<a href=\"https://doi.org/10.7554/eLife.66483\">10.7554/eLife.66483</a>","apa":"Pulgar, E., Schwayer, C., Guerrero, N., López, L., Márquez, S., Härtel, S., … Concha, M. L. (2021). Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.66483\">https://doi.org/10.7554/eLife.66483</a>","ieee":"E. Pulgar <i>et al.</i>, “Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021."},"date_published":"2021-08-27T00:00:00Z","publisher":"eLife Sciences Publications","file_date_updated":"2022-05-13T08:03:37Z","department":[{"_id":"CaHe"}],"doi":"10.7554/eLife.66483","scopus_import":"1","project":[{"call_identifier":"H2020","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"}],"_id":"9999","month":"08","oa_version":"Published Version","volume":10,"date_created":"2021-09-12T22:01:23Z","date_updated":"2025-04-14T07:46:58Z","quality_controlled":"1","keyword":["cell delamination","apical constriction","dragging","mechanical forces","collective 18 locomotion","dorsal forerunner cells","zebrafish"],"type":"journal_article","has_accepted_license":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"}}]