---
OA_type: closed access
_id: '3804'
abstract:
- lang: eng
  text: Kv3 channels are thought to be essential for the fast-spiking (FS) phenotype
    in GABAergic interneurons, but how these channels confer the ability to generate
    action potentials (APs) at high frequency is unknown. To address this question,
    we developed a fast dynamic-clamp system (approximately 50 kHz) that allowed us
    to add a Kv3 model conductance to CA1 oriens alveus (OA) interneurons in hippocampal
    slices. Selective pharmacological block of Kv3 channels by 0.3 mm 4-aminopyridine
    or 1 mm tetraethylammonium ions led to a marked broadening of APs during trains
    of short stimuli and a reduction in AP frequency during 1 sec stimuli. The addition
    of artificial Kv3 conductance restored the original AP pattern. Subtraction of
    Kv3 conductance by dynamic clamp mimicked the effects of the blockers. Application
    of artificial Kv3 conductance also led to FS in OA interneurons after complete
    K+ channel block and even induced FS in hippocampal pyramidal neurons in the absence
    of blockers. Adding artificial Kv3 conductance with altered deactivation kinetics
    revealed a nonmonotonic relationship between mean AP frequency and deactivation
    rate, with a maximum slightly above the original value. Insertion of artificial
    Kv3 conductance with either lowered activation threshold or inactivation also
    led to a reduction in the mean AP frequency. However, the mechanisms were distinct.
    Shifting the activation threshold induced adaptation, whereas adding inactivation
    caused frequency-dependent AP broadening. In conclusion, Kv3 channels are necessary
    for the FS phenotype of OA interneurons, and several of their gating properties
    appear to be optimized for high-frequency repetitive activity.
article_processing_charge: No
article_type: original
author:
- first_name: Cheng
  full_name: Lien, Cheng
  last_name: Lien
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: Lien C, Jonas PM. Kv3 potassium conductance is necessary and kinetically optimized
    for high-frequency action potential generation in hippocampal interneurons. <i>Journal
    of Neuroscience</i>. 2003;23(6):2058-2068. doi:<a href="https://doi.org/10.1523/JNEUROSCI.23-06-02058.2003">10.1523/JNEUROSCI.23-06-02058.2003</a>
  apa: Lien, C., &#38; Jonas, P. M. (2003). Kv3 potassium conductance is necessary
    and kinetically optimized for high-frequency action potential generation in hippocampal
    interneurons. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href="https://doi.org/10.1523/JNEUROSCI.23-06-02058.2003">https://doi.org/10.1523/JNEUROSCI.23-06-02058.2003</a>
  chicago: Lien, Cheng, and Peter M Jonas. “Kv3 Potassium Conductance Is Necessary
    and Kinetically Optimized for High-Frequency Action Potential Generation in Hippocampal
    Interneurons.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 2003.
    <a href="https://doi.org/10.1523/JNEUROSCI.23-06-02058.2003">https://doi.org/10.1523/JNEUROSCI.23-06-02058.2003</a>.
  ieee: C. Lien and P. M. Jonas, “Kv3 potassium conductance is necessary and kinetically
    optimized for high-frequency action potential generation in hippocampal interneurons,”
    <i>Journal of Neuroscience</i>, vol. 23, no. 6. Society for Neuroscience, pp.
    2058–68, 2003.
  ista: Lien C, Jonas PM. 2003. Kv3 potassium conductance is necessary and kinetically
    optimized for high-frequency action potential generation in hippocampal interneurons.
    Journal of Neuroscience. 23(6), 2058–68.
  mla: Lien, Cheng, and Peter M. Jonas. “Kv3 Potassium Conductance Is Necessary and
    Kinetically Optimized for High-Frequency Action Potential Generation in Hippocampal
    Interneurons.” <i>Journal of Neuroscience</i>, vol. 23, no. 6, Society for Neuroscience,
    2003, pp. 2058–68, doi:<a href="https://doi.org/10.1523/JNEUROSCI.23-06-02058.2003">10.1523/JNEUROSCI.23-06-02058.2003</a>.
  short: C. Lien, P.M. Jonas, Journal of Neuroscience 23 (2003) 2058–68.
date_created: 2018-12-11T12:05:16Z
date_published: 2003-03-15T00:00:00Z
date_updated: 2026-05-08T09:38:20Z
day: '15'
doi: 10.1523/JNEUROSCI.23-06-02058.2003
extern: '1'
external_id:
  pmid:
  - '12657664'
fulldoi: https://doi.org/10.1523/JNEUROSCI.23-06-02058.2003
intvolume: '        23'
issue: '6'
keyword:
- Kv3 channels
- dynamic clamp
- fast spiking
- deactivation kinetics
- OA interneurons
- hippocampal slices
- two electrode current clamp
language:
- iso: eng
month: '03'
oa_version: None
page: 2058 - 68
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
  eissn:
  - 1529-2401
  issn:
  - 0270-6474
publication_status: published
publisher: Society for Neuroscience
publist_id: '2406'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Kv3 potassium conductance is necessary and kinetically optimized for high-frequency
  action potential generation in hippocampal interneurons
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 23
year: '2003'
...