[{"citation":{"ieee":"L. Sun <i>et al.</i>, “Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis,” <i>The Plant Journal</i>, vol. 127, no. 1. Wiley, 2026.","short":"L. Sun, W. Jia, Y. Mao, X. Li, M. Kong, J. She, J. Friml, S. Tan, The Plant Journal 127 (2026).","ama":"Sun L, Jia W, Mao Y, et al. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. <i>The Plant Journal</i>. 2026;127(1). doi:<a href=\"https://doi.org/10.1111/tpj.71042\">10.1111/tpj.71042</a>","ista":"Sun L, Jia W, Mao Y, Li X, Kong M, She J, Friml J, Tan S. 2026. Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. The Plant Journal. 127(1), e71042.","apa":"Sun, L., Jia, W., Mao, Y., Li, X., Kong, M., She, J., … Tan, S. (2026). Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis. <i>The Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.71042\">https://doi.org/10.1111/tpj.71042</a>","chicago":"Sun, Lianghanxiao, Wenxin Jia, Yanbo Mao, Xin Li, Mengjuan Kong, Ji She, Jiří Friml, and Shutang Tan. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” <i>The Plant Journal</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/tpj.71042\">https://doi.org/10.1111/tpj.71042</a>.","mla":"Sun, Lianghanxiao, et al. “Regulation of Shoot Gravitropism and Branching Angle by the GRV2-SAC1 Axis in Arabidopsis.” <i>The Plant Journal</i>, vol. 127, no. 1, e71042, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/tpj.71042\">10.1111/tpj.71042</a>."},"OA_type":"closed access","status":"public","day":"01","department":[{"_id":"JiFr"}],"publisher":"Wiley","date_updated":"2026-07-20T13:53:44Z","month":"07","language":[{"iso":"eng"}],"date_published":"2026-07-01T00:00:00Z","acknowledgement":"We acknowledge Prof. Dolf Weijers (Wageningen University), Prof.Karin Schumacher (Heidelberg University), Prof. Yohann Boutt ´e(Universit ´e de Bordeaux), and Prof. Jinbo Shen (Zhejiang A&FUniversity) for providing published plasmids and Arabidopsislines. We thank Dr. Gergely Moln ´ar (ISTA) for help with NGS dataanalysis, and Prof. Jianru Zuo (IGDB, CAS), Prof. Chengbin Xiang(USTC), and Prof. Zhong Zhao (USTC) for critical comments onthe manuscript. We thank the staff members of the Mass Spec-trometry System at the National Facility for Protein Science inShanghai (NFPS), Zhangjiang Lab, China for providing technicalsupport and assistance in data collection and analysis. This workwas supported by grants from the National Natural Science Foun-dation of China (32570366, and 32321001 to ST), the Natural Sci-ence Foundation of Anhui Province (2508085QC070 to MK), theFundamental Research Funds for the Central Universities(WK9100250095 to MK, and WK9100000021 to ST), the ForestryBureau of Anhui Province (AHLYJBGS-2024-01 to ST), the Centerfor Advanced Interdisciplinary Science and Biomedicine of IHM,Division of Life Sciences and Medicine, University of Science andTechnology of China (QYPY20220012 to ST), the USTC ResearchFunds of the Double First-Class Initiative (YD9100002016 to ST),and start-up funding from the University of Science and Technol-ogy of China and the Chinese Academy of Sciences(GG9100007007, KY9100000026, KY9100000051, XKTS-202591014,XKTS-2026910122, and KJ2070000079 to ST).","type":"journal_article","article_type":"original","oa_version":"None","intvolume":"       127","_id":"22366","scopus_import":"1","quality_controlled":"1","supplementarymaterial":"yes","researchdata_availability":"upon request","article_number":"e71042","year":"2026","date_created":"2026-07-19T22:01:47Z","publication":"The Plant Journal","das_tickbox":"1","issue":"1","doi":"10.1111/tpj.71042","publication_identifier":{"eissn":["1365-313X"],"issn":["0960-7412"]},"dataavailabilitystatement":"Biological materials (seeds, plasmids) are available upon request from ST (sttan@ustc.edu.cn). The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.","abstract":[{"lang":"eng","text":"Gravitropism is a fundamental adaptive response in plants that enables directional growth to optimize resource acquisition. In this study, we employed forward genetic screening to identify Arabidopsis mutants with defective hypocotyl gravitropism and isolated the short and agravitropic hypocotyl in dark1 (sad1) mutant, which carries a point mutation (G110E) in the SAC1 gene encoding a phosphoinositide phosphatase. Deficiency of SAC1 disrupted gravity-induced polar localization of PIN3 in endodermal cells, impairing auxin redistribution and leading to hypocotyl gravitropism defects. Subcellular localization analysis revealed that SAC1 is partially localized to the PVC/tonoplast and participates in late endosomal trafficking. The sac1 mutation leads to abnormal vacuolar morphology, which is associated with defects in amyloplast sedimentation during the gravitropic response in Arabidopsis shoots. We further revealed that SAC1 interacts with GRV2, a key regulator of the late endocytic pathway, and that both proteins cooperatively regulate shoot gravitropism. In summary, this study identified SAC1 as a regulator of shoot gravitropism, revealing its important role in modulating vacuolar homeostasis, amyloplast sedimentation, PIN3 trafficking, and auxin distribution. These findings provide insights into the molecular mechanisms linking membrane transport to environmental adaptation in plants."}],"external_id":{"pmid":["42438075"]},"publication_status":"published","article_processing_charge":"No","title":"Regulation of shoot gravitropism and branching angle by the GRV2-SAC1 axis in Arabidopsis","keyword":["auxin","SAC1","GRV2","PIN3","vacuole","gravitropism","Arabidopsis"],"volume":127,"author":[{"full_name":"Sun, Lianghanxiao","first_name":"Lianghanxiao","last_name":"Sun"},{"last_name":"Jia","first_name":"Wenxin","full_name":"Jia, Wenxin"},{"full_name":"Mao, Yanbo","last_name":"Mao","first_name":"Yanbo"},{"first_name":"Xin","last_name":"Li","full_name":"Li, Xin"},{"first_name":"Mengjuan","last_name":"Kong","full_name":"Kong, Mengjuan"},{"full_name":"She, Ji","last_name":"She","first_name":"Ji"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"},{"first_name":"Shutang","last_name":"Tan","full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0471-8285"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1},{"has_accepted_license":"1","ddc":["575"],"corr_author":"1","quality_controlled":"1","file":[{"checksum":"6b7055cf89f1b7ed8594c3fdf56f000b","relation":"main_file","access_level":"open_access","file_size":2162247,"date_updated":"2021-09-07T09:04:53Z","file_id":"9988","creator":"cchlebak","date_created":"2021-09-06T12:50:19Z","file_name":"2021_IntJMolecularSciences_Velasquez.pdf","content_type":"application/pdf"}],"publication_identifier":{"eissn":["1422-0067"],"issn":["1661-6596"]},"doi":"10.3390/ijms22179222","issue":"17","publication":"International Journal of Molecular Sciences","date_created":"2021-09-05T22:01:24Z","year":"2021","article_number":"9222","keyword":["auxin","growth","cell wall","xyloglucans","hypocotyls","gravitropism"],"title":"Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants","article_processing_charge":"Yes","external_id":{"pmid":["34502129"],"isi":["000694347100001"]},"publication_status":"published","abstract":[{"lang":"eng","text":"Size control is a fundamental question in biology, showing incremental complexity in plants, whose cells possess a rigid cell wall. The phytohormone auxin is a vital growth regulator with central importance for differential growth control. Our results indicate that auxin-reliant growth programs affect the molecular complexity of xyloglucans, the major type of cell wall hemicellulose in eudicots. Auxin-dependent induction and repression of growth coincide with reduced and enhanced molecular complexity of xyloglucans, respectively. In agreement with a proposed function in growth control, genetic interference with xyloglucan side decorations distinctly modulates auxin-dependent differential growth rates. Our work proposes that auxin-dependent growth programs have a spatially defined effect on xyloglucan’s molecular structure, which in turn affects cell wall mechanics and specifies differential, gravitropic hypocotyl growth."}],"pmid":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Silvia Melina","last_name":"Velasquez","full_name":"Velasquez, Silvia Melina"},{"full_name":"Guo, Xiaoyuan","last_name":"Guo","first_name":"Xiaoyuan"},{"orcid":"0000-0003-4675-6893","id":"460C6802-F248-11E8-B48F-1D18A9856A87","full_name":"Gallemi, Marçal","last_name":"Gallemi","first_name":"Marçal"},{"last_name":"Aryal","first_name":"Bibek","full_name":"Aryal, Bibek"},{"full_name":"Venhuizen, Peter","first_name":"Peter","last_name":"Venhuizen"},{"full_name":"Barbez, Elke","last_name":"Barbez","first_name":"Elke"},{"last_name":"Dünser","first_name":"Kai Alexander","full_name":"Dünser, Kai Alexander"},{"full_name":"Darino, Martin","first_name":"Martin","last_name":"Darino"},{"full_name":"Pӗnčík, Aleš","last_name":"Pӗnčík","first_name":"Aleš"},{"first_name":"Ondřej","last_name":"Novák","full_name":"Novák, Ondřej"},{"first_name":"Maria","last_name":"Kalyna","full_name":"Kalyna, Maria"},{"first_name":"Gregory","last_name":"Mouille","full_name":"Mouille, Gregory"},{"first_name":"Eva","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Bhalerao, Rishikesh P.","last_name":"Bhalerao","first_name":"Rishikesh P."},{"full_name":"Mravec, Jozef","last_name":"Mravec","first_name":"Jozef"},{"first_name":"Jürgen","last_name":"Kleine-Vehn","full_name":"Kleine-Vehn, Jürgen"}],"volume":22,"citation":{"ama":"Velasquez SM, Guo X, Gallemi M, et al. Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. <i>International Journal of Molecular Sciences</i>. 2021;22(17). doi:<a href=\"https://doi.org/10.3390/ijms22179222\">10.3390/ijms22179222</a>","ista":"Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA, Darino M, Pӗnčík A, Novák O, Kalyna M, Mouille G, Benková E, Bhalerao RP, Mravec J, Kleine-Vehn J. 2021. Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. International Journal of Molecular Sciences. 22(17), 9222.","short":"S.M. Velasquez, X. Guo, M. Gallemi, B. Aryal, P. Venhuizen, E. Barbez, K.A. Dünser, M. Darino, A. Pӗnčík, O. Novák, M. Kalyna, G. Mouille, E. Benková, R.P. Bhalerao, J. Mravec, J. Kleine-Vehn, International Journal of Molecular Sciences 22 (2021).","ieee":"S. M. Velasquez <i>et al.</i>, “Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants,” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 17. MDPI, 2021.","mla":"Velasquez, Silvia Melina, et al. “Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 17, 9222, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/ijms22179222\">10.3390/ijms22179222</a>.","apa":"Velasquez, S. M., Guo, X., Gallemi, M., Aryal, B., Venhuizen, P., Barbez, E., … Kleine-Vehn, J. (2021). Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms22179222\">https://doi.org/10.3390/ijms22179222</a>","chicago":"Velasquez, Silvia Melina, Xiaoyuan Guo, Marçal Gallemi, Bibek Aryal, Peter Venhuizen, Elke Barbez, Kai Alexander Dünser, et al. “Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal of Molecular Sciences</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/ijms22179222\">https://doi.org/10.3390/ijms22179222</a>."},"day":"26","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"date_published":"2021-08-26T00:00:00Z","language":[{"iso":"eng"}],"month":"08","date_updated":"2024-10-09T21:00:50Z","department":[{"_id":"EvBe"}],"publisher":"MDPI","isi":1,"file_date_updated":"2021-09-07T09:04:53Z","oa":1,"scopus_import":"1","_id":"9986","oa_version":"Published Version","intvolume":"        22","article_type":"original","acknowledgement":"We are grateful to Paul Knox, Markus Pauly, Malcom O’Neill, and Ignacio Zarra for providing published material; the BOKU-VIBT Imaging Center for access and M. Debreczeny for expertise; J.I. Thaker and Georg Seifert for critical reading.\r\n","type":"journal_article"}]