[{"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","page":"104","has_accepted_license":"1","project":[{"name":"International IST Doctoral Program","call_identifier":"H2020","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"}],"date_published":"2023-11-30T00:00:00Z","oa_version":"Published Version","year":"2023","date_updated":"2026-04-07T13:29:59Z","day":"30","type":"dissertation","publisher":"Institute of Science and Technology Austria","status":"public","doi":"10.15479/at:ista:14641","OA_place":"publisher","alternative_title":["ISTA Thesis"],"ddc":["570"],"article_processing_charge":"No","citation":{"ista":"Hennessey-Wesen M. 2023. Adaptive mutation in E. coli modulated by luxS. Institute of Science and Technology Austria.","chicago":"Hennessey-Wesen, Mike. “Adaptive Mutation in E. Coli Modulated by LuxS.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/at:ista:14641\">https://doi.org/10.15479/at:ista:14641</a>.","apa":"Hennessey-Wesen, M. (2023). <i>Adaptive mutation in E. coli modulated by luxS</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:14641\">https://doi.org/10.15479/at:ista:14641</a>","ama":"Hennessey-Wesen M. Adaptive mutation in E. coli modulated by luxS. 2023. doi:<a href=\"https://doi.org/10.15479/at:ista:14641\">10.15479/at:ista:14641</a>","ieee":"M. Hennessey-Wesen, “Adaptive mutation in E. coli modulated by luxS,” Institute of Science and Technology Austria, 2023.","short":"M. Hennessey-Wesen, Adaptive Mutation in E. Coli Modulated by LuxS, Institute of Science and Technology Austria, 2023.","mla":"Hennessey-Wesen, Mike. <i>Adaptive Mutation in E. Coli Modulated by LuxS</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/at:ista:14641\">10.15479/at:ista:14641</a>."},"_id":"14641","title":"Adaptive mutation in E. coli modulated by luxS","month":"11","author":[{"full_name":"Hennessey-Wesen, Mike","last_name":"Hennessey-Wesen","first_name":"Mike","id":"3F338C72-F248-11E8-B48F-1D18A9856A87"}],"keyword":["microfluidics","miceobiology","mutations","quorum sensing"],"file_date_updated":"2025-07-17T11:20:25Z","ec_funded":1,"file":[{"embargo_to":"open_access","access_level":"closed","relation":"source_file","file_size":46405919,"file_name":"mike_thesis_v06-12-2023.odt","creator":"mhenness","file_id":"14648","date_created":"2023-12-06T13:13:26Z","date_updated":"2024-11-30T23:30:05Z","content_type":"application/vnd.oasis.opendocument.text","checksum":"4127c285b34f4bf7fb31ef24f9d14c25"},{"date_updated":"2025-07-17T11:20:25Z","content_type":"application/pdf","checksum":"f5203a61eddaf35235bbc51904d73982","file_size":21282155,"date_created":"2023-12-06T13:14:15Z","file_id":"14649","file_name":"mike_thesis_v06-12-2023.pdf","creator":"mhenness","embargo_to":"open_access","relation":"main_file","access_level":"closed","embargo":"2026-07-18"},{"creator":"cchlebak","file_name":"2023_Hennessey_Michael_Thesis_print.pdf","date_created":"2025-05-20T12:59:12Z","file_id":"19720","file_size":45847968,"description":"for printing purposes only","content_type":"application/pdf","checksum":"902102d26d30e74dbd6cdd70a65820c3","date_updated":"2025-05-20T22:31:34Z","access_level":"closed","relation":"other","embargo_to":"open_access","title":"Print version"}],"publication_identifier":{"issn":["2663-337X"]},"department":[{"_id":"GradSch"},{"_id":"BjHo"}],"date_created":"2023-12-04T13:17:37Z","language":[{"iso":"eng"}],"corr_author":"1","degree_awarded":"PhD","publication_status":"published","abstract":[{"lang":"eng","text":"Mutation rates represent the net result of complex interactions among various\r\ncellular processes and can dramatically influence the evolutionary fate of\r\nmicrobial populations. However, many popular techniques used to study\r\nmutations are subject to the confounding effects of heredity and the subtleties\r\nof adaptation to selection, all of which make it difficult to observe any dynamic\r\nresponses of mutation rates to fitness challenges. Furthermore, in spite of the\r\nubiquity of quorum sensing systems across the bacterial domain and relevance\r\nfor many physiological behaviors, the effects of such mechanisms on mutation\r\nrate and adaptation remain poorly understood. In the following work, I\r\npresent the development of a microfluidic droplet-based method to measure\r\nsingle base-pair mutation rates in growing populations of the bacterium\r\nEscherichia coli. I use this method to observe a stress-induced increase in\r\nmutation rate that is mediated by luxS, a highly conserved bacterial quorum\r\nsensing component. I also show that the aforementioned increase in mutation\r\nrate, and its associated control by luxS, corresponds to a higher degree of\r\nadaptability under competitive environments."}],"supervisor":[{"orcid":"0000-0003-2057-2754","full_name":"Hof, Björn","last_name":"Hof","first_name":"Björn","id":"3A374330-F248-11E8-B48F-1D18A9856A87"}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"CampIT"}]}]