[{"day":"23","file_date_updated":"2026-03-24T08:34:37Z","PlanS_conform":"1","department":[{"_id":"JiFr"}],"type":"journal_article","page":"1468-1480.e6","date_published":"2026-03-23T00:00:00Z","publication_status":"published","file":[{"success":1,"date_created":"2026-03-24T08:34:37Z","date_updated":"2026-03-24T08:34:37Z","relation":"main_file","file_name":"2026_CurrentBiology_Li.pdf","access_level":"open_access","file_size":12986894,"creator":"dernst","checksum":"fe6c41fdab58a55df5f2a5860c02acdc","content_type":"application/pdf","file_id":"21496"}],"intvolume":"        36","article_type":"original","oa_version":"Published Version","author":[{"first_name":"Mingyue","full_name":"Li, Mingyue","last_name":"Li","id":"01f96916-0235-11eb-9379-a323192643b7"},{"last_name":"Rydza","full_name":"Rydza, Nikola","first_name":"Nikola"},{"last_name":"Mazur","first_name":"Ewa","full_name":"Mazur, Ewa"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","last_name":"Molnar","first_name":"Gergely","full_name":"Molnar, Gergely"},{"first_name":"Tomasz","full_name":"Nodzyński, Tomasz","last_name":"Nodzyński"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří"}],"publication_identifier":{"issn":["0960-9822"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","acknowledgement":"We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9 system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof. Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions, and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging & Optics Facility, and the Lab Support Facility at the Institute of Science and Technology Austria. This research was supported by the Scientific Service Units (SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF) and the Lab Support Facility (LSF). The work conducted by the Friml group was funded by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS) and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant Sciences Core Facility of CEITEC Masaryk University. E.M. received support from the National Science Centre (NCN), Poland, through the OPUS call within the Weave programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.","date_updated":"2026-03-24T08:36:40Z","publication":"Current Biology","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"Bio"},{"_id":"LifeSc"}],"oa":1,"external_id":{"pmid":["41831441"]},"OA_type":"hybrid","ddc":["580"],"_id":"21490","article_processing_charge":"Yes (via OA deal)","quality_controlled":"1","volume":36,"language":[{"iso":"eng"}],"doi":"10.1016/j.cub.2026.02.023","OA_place":"publisher","status":"public","date_created":"2026-03-23T15:11:16Z","project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Identification of a novel regulator in auxin canalization","_id":"bd906599-d553-11ed-ba76-abf8547645d7","grant_number":"E271"}],"month":"03","citation":{"ama":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. 2026;36(6):1468-1480.e6. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>","ieee":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization,” <i>Current Biology</i>, vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026.","mla":"Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>, vol. 36, no. 6, Elsevier, 2026, p. 1468–1480.e6, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">10.1016/j.cub.2026.02.023</a>.","apa":"Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., &#38; Friml, J. (2026). Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>","short":"M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology 36 (2026) 1468–1480.e6.","chicago":"Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński, and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters for Auxin Canalization.” <i>Current Biology</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.02.023\">https://doi.org/10.1016/j.cub.2026.02.023</a>.","ista":"Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization. Current Biology. 36(6), 1468–1480.e6."},"issue":"6","abstract":[{"lang":"eng","text":"Auxin canalization is a self-organizing process that governs the flexible formation of vasculature by reinforcing the formation of auxin transport channels. A key prerequisite is the feedback between auxin signaling and directional auxin transport, mediated by PIN transporters. Despite the developmental importance of canalization, the molecular components linking auxin perception to the regulation of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel and essential component of auxin canalization that links intracellular auxin signaling with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23 transcriptional auxin signaling. tow mutants exhibit defects in regeneration and de novo vasculature formation, along with impaired formation of polarized, PIN-expressing auxin channels. At the subcellular level, these mutants display disrupted auxin-induced PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes predominantly to the plasma membrane, where it interacts with receptor-like kinases involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at the cell surface. Together, our findings identify TOW as a molecular link between intracellular and cell surface auxin signaling mechanisms that converge on PIN trafficking and polarity, providing new insights into how auxin signaling regulates directional auxin transport for the self-organizing formation of vasculature during flexible plant development."}],"publisher":"Elsevier","title":"Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin canalization","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"year":"2026","has_accepted_license":"1","pmid":1},{"PlanS_conform":"1","file_date_updated":"2026-06-02T14:33:55Z","day":"08","type":"journal_article","department":[{"_id":"JiFr"}],"date_published":"2026-05-08T00:00:00Z","DOAJ_listed":"1","publication_status":"published","intvolume":"        12","file":[{"file_name":"2026_ScienceAdv_Li2.pdf","file_size":2014452,"access_level":"open_access","creator":"dernst","checksum":"75b8ef2db078652c750e34e9cd98a808","file_id":"21941","content_type":"application/pdf","date_created":"2026-06-02T14:33:55Z","success":1,"date_updated":"2026-06-02T14:33:55Z","relation":"main_file"}],"article_number":"aea7828","article_type":"original","oa_version":"Published Version","author":[{"full_name":"Li, Mingyue","first_name":"Mingyue","id":"01f96916-0235-11eb-9379-a323192643b7","last_name":"Li"},{"last_name":"Chodasiewicz","first_name":"Monika","full_name":"Chodasiewicz, Monika"},{"last_name":"Muraleedharan","first_name":"Malavika","full_name":"Muraleedharan, Malavika"},{"last_name":"Lopez","full_name":"Lopez, Israel M.","first_name":"Israel M."},{"first_name":"Michal","full_name":"Gorka, Michal","last_name":"Gorka"},{"full_name":"Kerber, Olga","first_name":"Olga","last_name":"Kerber"},{"last_name":"Alotaibi","first_name":"Saqer S.","full_name":"Alotaibi, Saqer S."},{"full_name":"Nelson, Andrew D.L.","first_name":"Andrew D.L.","last_name":"Nelson"},{"last_name":"Lenobel","first_name":"Rene","full_name":"Lenobel, Rene"},{"first_name":"Jaroslava","full_name":"Friedecká, Jaroslava","last_name":"Friedecká"},{"last_name":"Skirycz","first_name":"Aleksandra","full_name":"Skirycz, Aleksandra"},{"first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"publication_identifier":{"eissn":["2375-2548"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","date_updated":"2026-06-02T14:36:41Z","acknowledgement":" We thank J. Chai and D. Yu for providing the MBP-fused L7TIR plasmid and K. Jaworski (Nicolaus Copernicus University) for the GST-­HpAC1 plasmid. We also thank M. Randuch and L. Fiedler for providing vectors for recombinant AFB5 and ADCY. We are also grateful to E. Dutkiewicz, L. Trübestein, N. Krasnici and A. Michaelis for excellent technical\r\nassistance. We acknowledge the support of the LSF Mass Spectrometry Service and the Lab\r\nSupport Facility at the Institute of Science and Technology Austria for their contributions,\r\nincluding consultation on size exclusion chromatography, LC/MS experimental design,\r\nmetabolomics sample preparation, LC/MS method optimization, data acquisition, raw data\r\nanalysis, and absolute quantification. This project is supported by the European\r\nResearch Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogram (101142681 CYNIPS) and Austrian Science Fund (FWF; P 37051-B), both to J.Friml.\r\nWe acknowledge the generous support of the Taif University Researchers Supporting\r\nProject: TURSP-­HC2022/02 and Max-Planck-Society to A.S. ","acknowledged_ssus":[{"_id":"MassSpec"},{"_id":"LifeSc"}],"publication":"Science Advances","oa":1,"external_id":{"pmid":["42102187"]},"OA_type":"gold","ddc":["580"],"_id":"21914","quality_controlled":"1","article_processing_charge":"Yes","language":[{"iso":"eng"}],"volume":12,"OA_place":"publisher","doi":"10.1126/sciadv.aea7828","date_created":"2026-05-24T22:01:31Z","project":[{"grant_number":"101142681","_id":"8f347782-16d5-11f0-9cad-8c19706ee739","name":"Cyclic nucleotides as second messengers in plants"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"status":"public","issue":"19","citation":{"apa":"Li, M., Chodasiewicz, M., Muraleedharan, M., Lopez, I. M., Gorka, M., Kerber, O., … Friml, J. (2026). Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aea7828\">https://doi.org/10.1126/sciadv.aea7828</a>","mla":"Li, Mingyue, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” <i>Science Advances</i>, vol. 12, no. 19, aea7828, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aea7828\">10.1126/sciadv.aea7828</a>.","ieee":"M. Li <i>et al.</i>, “Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants,” <i>Science Advances</i>, vol. 12, no. 19. AAAS, 2026.","ama":"Li M, Chodasiewicz M, Muraleedharan M, et al. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. <i>Science Advances</i>. 2026;12(19). doi:<a href=\"https://doi.org/10.1126/sciadv.aea7828\">10.1126/sciadv.aea7828</a>","chicago":"Li, Mingyue, Monika Chodasiewicz, Malavika Muraleedharan, Israel M. Lopez, Michal Gorka, Olga Kerber, Saqer S. Alotaibi, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.aea7828\">https://doi.org/10.1126/sciadv.aea7828</a>.","ista":"Li M, Chodasiewicz M, Muraleedharan M, Lopez IM, Gorka M, Kerber O, Alotaibi SS, Nelson ADL, Lenobel R, Friedecká J, Skirycz A, Friml J. 2026. Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 12(19), aea7828.","short":"M. Li, M. Chodasiewicz, M. Muraleedharan, I.M. Lopez, M. Gorka, O. Kerber, S.S. Alotaibi, A.D.L. Nelson, R. Lenobel, J. Friedecká, A. Skirycz, J. Friml, Science Advances 12 (2026)."},"month":"05","abstract":[{"text":"Cyclic adenosine monophosphate (cAMP) is a fundamental second messenger involved in diverse signaling pathways across both animals and plants. While the role of 3′,5′-cAMP has been extensively characterized, the biological significance of its structural isomer, 2′,3′-cAMP, remains largely unexplored, particularly in plants. Here, we show that 2′,3′-cAMP and 3′,5′-cAMP represent parallel signaling systems in Arabidopsis thaliana, with different enzymatic origins and largely distinct downstream effects. In vitro enzymatic assays show that plant adenylate cyclases (ACs), including AFB5 and HpAC1, produce specifically 3′,5′-cAMP from ATP, whereas the TIR domain of protein L7 also catalyzes the formation of 2′,3′-cAMP from RNA. Comprehensive multiomics analyses reveal that two isomers elicit distinct yet partially overlapping metabolic, proteomic, and transcriptional response: 2′,3′-cAMP activates broad, stress-adaptive gene expression reprogramming, while 3′,5′-cAMP fine-tunes responses related to nutrient status and cellular homeostasis. Our findings establish the existence of dual cAMP signaling systems in plants, each with specialized functions and provide insights into the complex regulatory networks governing plant physiology.","lang":"eng"}],"publisher":"AAAS","title":"Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plants","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"scopus_import":"1","year":"2026","has_accepted_license":"1","pmid":1}]