---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21490'
abstract:
- lang: eng
text: Auxin canalization is a self-organizing process that governs the flexible
formation of vasculature by reinforcing the formation of auxin transport channels.
A key prerequisite is the feedback between auxin signaling and directional auxin
transport, mediated by PIN transporters. Despite the developmental importance
of canalization, the molecular components linking auxin perception to the regulation
of PIN auxin transporters remain poorly understood. Here, we identify TOW, a novel
and essential component of auxin canalization that links intracellular auxin signaling
with cell surface auxin perception. TOW is regulated downstream of TIR1/AFB-Aux/IAA-WRKY23
transcriptional auxin signaling. tow mutants exhibit defects in regeneration and
de novo vasculature formation, along with impaired formation of polarized, PIN-expressing
auxin channels. At the subcellular level, these mutants display disrupted auxin-induced
PIN polarization and altered PIN endocytic trafficking dynamics. TOW localizes
predominantly to the plasma membrane, where it interacts with receptor-like kinases
involved in auxin canalization, including the TMK1 auxin co-receptor and the CAMEL-CANAR
complex. TOW promotes PIN interaction with these kinases and stabilizes PINs at
the cell surface. Together, our findings identify TOW as a molecular link between
intracellular and cell surface auxin signaling mechanisms that converge on PIN
trafficking and polarity, providing new insights into how auxin signaling regulates
directional auxin transport for the self-organizing formation of vasculature during
flexible plant development.
acknowledged_ssus:
- _id: MassSpec
- _id: Bio
- _id: LifeSc
acknowledgement: We thank Dr. Z. Ge (ISTA) for providing vectors for the CRISPR-Cas9
system, Dr. Armel Nicolas and Dr. Bella Bruszel for phosphoproteomic analysis, Prof.
Michael Wrzaczek (Czech Academy of Sciences, Czechia) for valuable suggestions,
and Prof. Maciek Adamowski (University of Gdańsk) for technical assistance. We also
acknowledge the support of the Mass Spectrometry and Proteomics Facility, the Imaging
& Optics Facility, and the Lab Support Facility at the Institute of Science and
Technology Austria. This research was supported by the Scientific Service Units
(SSU) of ISTA, utilizing resources provided by the Imaging & Optics Facility (IOF)
and the Lab Support Facility (LSF). The work conducted by the Friml group was funded
by the European Research Council (ERC) under grant agreement no. 101142681 (CYNIPS)
and by the Austrian Science Fund (FWF) under project ESP271. We acknowledge the
core facility CELLIM supported by MEYS CR (LM2023050 Czech-BioImaging) and the Plant
Sciences Core Facility of CEITEC Masaryk University. E.M. received support from
the National Science Centre (NCN), Poland, through the OPUS call within the Weave
programme (grant no. 2021/43/I/NZ1/01835). T.N. received support from TowArds Next
GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes
Amos Comenius.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Mingyue
full_name: Li, Mingyue
id: 01f96916-0235-11eb-9379-a323192643b7
last_name: Li
- first_name: Nikola
full_name: Rydza, Nikola
last_name: Rydza
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. Receptor-like-kinase-interacting
protein TOW stabilizes PIN transporters for auxin canalization. Current Biology.
2026;36(6):1468-1480.e6. doi:10.1016/j.cub.2026.02.023
apa: Li, M., Rydza, N., Mazur, E., Molnar, G., Nodzyński, T., & Friml, J. (2026).
Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for auxin
canalization. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2026.02.023
chicago: Li, Mingyue, Nikola Rydza, Ewa Mazur, Gergely Molnar, Tomasz Nodzyński,
and Jiří Friml. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes PIN Transporters
for Auxin Canalization.” Current Biology. Elsevier, 2026. https://doi.org/10.1016/j.cub.2026.02.023.
ieee: M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, and J. Friml, “Receptor-like-kinase-interacting
protein TOW stabilizes PIN transporters for auxin canalization,” Current Biology,
vol. 36, no. 6. Elsevier, p. 1468–1480.e6, 2026.
ista: Li M, Rydza N, Mazur E, Molnar G, Nodzyński T, Friml J. 2026. Receptor-like-kinase-interacting
protein TOW stabilizes PIN transporters for auxin canalization. Current Biology.
36(6), 1468–1480.e6.
mla: Li, Mingyue, et al. “Receptor-like-Kinase-Interacting Protein TOW Stabilizes
PIN Transporters for Auxin Canalization.” Current Biology, vol. 36, no.
6, Elsevier, 2026, p. 1468–1480.e6, doi:10.1016/j.cub.2026.02.023.
short: M. Li, N. Rydza, E. Mazur, G. Molnar, T. Nodzyński, J. Friml, Current Biology
36 (2026) 1468–1480.e6.
corr_author: '1'
date_created: 2026-03-23T15:11:16Z
date_published: 2026-03-23T00:00:00Z
date_updated: 2026-03-24T08:36:40Z
day: '23'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.cub.2026.02.023
external_id:
pmid:
- '41831441'
file:
- access_level: open_access
checksum: fe6c41fdab58a55df5f2a5860c02acdc
content_type: application/pdf
creator: dernst
date_created: 2026-03-24T08:34:37Z
date_updated: 2026-03-24T08:34:37Z
file_id: '21496'
file_name: 2026_CurrentBiology_Li.pdf
file_size: 12986894
relation: main_file
success: 1
file_date_updated: 2026-03-24T08:34:37Z
has_accepted_license: '1'
intvolume: ' 36'
issue: '6'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 1468-1480.e6
pmid: 1
project:
- _id: 8f347782-16d5-11f0-9cad-8c19706ee739
grant_number: '101142681'
name: Cyclic nucleotides as second messengers in plants
- _id: bd906599-d553-11ed-ba76-abf8547645d7
grant_number: E271
name: Identification of a novel regulator in auxin canalization
publication: Current Biology
publication_identifier:
issn:
- 0960-9822
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Receptor-like-kinase-interacting protein TOW stabilizes PIN transporters for
auxin canalization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '21914'
abstract:
- lang: eng
text: 'Cyclic adenosine monophosphate (cAMP) is a fundamental second messenger involved
in diverse signaling pathways across both animals and plants. While the role of
3′,5′-cAMP has been extensively characterized, the biological significance of
its structural isomer, 2′,3′-cAMP, remains largely unexplored, particularly in
plants. Here, we show that 2′,3′-cAMP and 3′,5′-cAMP represent parallel signaling
systems in Arabidopsis thaliana, with different enzymatic origins and largely
distinct downstream effects. In vitro enzymatic assays show that plant adenylate
cyclases (ACs), including AFB5 and HpAC1, produce specifically 3′,5′-cAMP from
ATP, whereas the TIR domain of protein L7 also catalyzes the formation of 2′,3′-cAMP
from RNA. Comprehensive multiomics analyses reveal that two isomers elicit distinct
yet partially overlapping metabolic, proteomic, and transcriptional response:
2′,3′-cAMP activates broad, stress-adaptive gene expression reprogramming, while
3′,5′-cAMP fine-tunes responses related to nutrient status and cellular homeostasis.
Our findings establish the existence of dual cAMP signaling systems in plants,
each with specialized functions and provide insights into the complex regulatory
networks governing plant physiology.'
acknowledged_ssus:
- _id: MassSpec
- _id: LifeSc
acknowledgement: " We thank J. Chai and D. Yu for providing the MBP-fused L7TIR plasmid
and K. Jaworski (Nicolaus Copernicus University) for the GST-HpAC1 plasmid. We
also thank M. Randuch and L. Fiedler for providing vectors for recombinant AFB5
and ADCY. We are also grateful to E. Dutkiewicz, L. Trübestein, N. Krasnici and
A. Michaelis for excellent technical\r\nassistance. We acknowledge the support of
the LSF Mass Spectrometry Service and the Lab\r\nSupport Facility at the Institute
of Science and Technology Austria for their contributions,\r\nincluding consultation
on size exclusion chromatography, LC/MS experimental design,\r\nmetabolomics sample
preparation, LC/MS method optimization, data acquisition, raw data\r\nanalysis,
and absolute quantification. This project is supported by the European\r\nResearch
Council (ERC) under the European Union’s Horizon 2020 research and innovation\r\nprogram
(101142681 CYNIPS) and Austrian Science Fund (FWF; P 37051-B), both to J.Friml.\r\nWe
acknowledge the generous support of the Taif University Researchers Supporting\r\nProject:
TURSP-HC2022/02 and Max-Planck-Society to A.S. "
article_number: aea7828
article_processing_charge: Yes
article_type: original
author:
- first_name: Mingyue
full_name: Li, Mingyue
id: 01f96916-0235-11eb-9379-a323192643b7
last_name: Li
- first_name: Monika
full_name: Chodasiewicz, Monika
last_name: Chodasiewicz
- first_name: Malavika
full_name: Muraleedharan, Malavika
last_name: Muraleedharan
- first_name: Israel M.
full_name: Lopez, Israel M.
last_name: Lopez
- first_name: Michal
full_name: Gorka, Michal
last_name: Gorka
- first_name: Olga
full_name: Kerber, Olga
last_name: Kerber
- first_name: Saqer S.
full_name: Alotaibi, Saqer S.
last_name: Alotaibi
- first_name: Andrew D.L.
full_name: Nelson, Andrew D.L.
last_name: Nelson
- first_name: Rene
full_name: Lenobel, Rene
last_name: Lenobel
- first_name: Jaroslava
full_name: Friedecká, Jaroslava
last_name: Friedecká
- first_name: Aleksandra
full_name: Skirycz, Aleksandra
last_name: Skirycz
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li M, Chodasiewicz M, Muraleedharan M, et al. Biogenesis and downstream effects
of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances. 2026;12(19).
doi:10.1126/sciadv.aea7828
apa: Li, M., Chodasiewicz, M., Muraleedharan, M., Lopez, I. M., Gorka, M., Kerber,
O., … Friml, J. (2026). Biogenesis and downstream effects of 3’,5’ and 2’,3’ cAMP
isomers in plants. Science Advances. AAAS. https://doi.org/10.1126/sciadv.aea7828
chicago: Li, Mingyue, Monika Chodasiewicz, Malavika Muraleedharan, Israel M. Lopez,
Michal Gorka, Olga Kerber, Saqer S. Alotaibi, et al. “Biogenesis and Downstream
Effects of 3’,5’ and 2’,3’ CAMP Isomers in Plants.” Science Advances. AAAS,
2026. https://doi.org/10.1126/sciadv.aea7828.
ieee: M. Li et al., “Biogenesis and downstream effects of 3’,5’ and 2’,3’
cAMP isomers in plants,” Science Advances, vol. 12, no. 19. AAAS, 2026.
ista: Li M, Chodasiewicz M, Muraleedharan M, Lopez IM, Gorka M, Kerber O, Alotaibi
SS, Nelson ADL, Lenobel R, Friedecká J, Skirycz A, Friml J. 2026. Biogenesis and
downstream effects of 3’,5’ and 2’,3’ cAMP isomers in plants. Science Advances.
12(19), aea7828.
mla: Li, Mingyue, et al. “Biogenesis and Downstream Effects of 3’,5’ and 2’,3’ CAMP
Isomers in Plants.” Science Advances, vol. 12, no. 19, aea7828, AAAS, 2026,
doi:10.1126/sciadv.aea7828.
short: M. Li, M. Chodasiewicz, M. Muraleedharan, I.M. Lopez, M. Gorka, O. Kerber,
S.S. Alotaibi, A.D.L. Nelson, R. Lenobel, J. Friedecká, A. Skirycz, J. Friml,
Science Advances 12 (2026).
corr_author: '1'
date_created: 2026-05-24T22:01:31Z
date_published: 2026-05-08T00:00:00Z
date_updated: 2026-06-02T14:36:41Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1126/sciadv.aea7828
external_id:
pmid:
- '42102187'
file:
- access_level: open_access
checksum: 75b8ef2db078652c750e34e9cd98a808
content_type: application/pdf
creator: dernst
date_created: 2026-06-02T14:33:55Z
date_updated: 2026-06-02T14:33:55Z
file_id: '21941'
file_name: 2026_ScienceAdv_Li2.pdf
file_size: 2014452
relation: main_file
success: 1
file_date_updated: 2026-06-02T14:33:55Z
has_accepted_license: '1'
intvolume: ' 12'
issue: '19'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 8f347782-16d5-11f0-9cad-8c19706ee739
grant_number: '101142681'
name: Cyclic nucleotides as second messengers in plants
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: AAAS
quality_controlled: '1'
scopus_import: '1'
status: public
title: Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2026'
...