---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20404'
abstract:
- lang: eng
text: Collagens are fundamental components of extracellular matrices, requiring
precise intracellular post-translational modifications for proper function. Among
the modifications, prolyl 4-hydroxylation is critical to stabilise the collagen
triple helix. In humans, this reaction is mediated by collagen prolyl 4-hydroxylases
(P4Hs). While humans possess three genes encoding these enzymes (P4H⍺s), Drosophila
melanogaster harbour at least 26 candidates for collagen P4H⍺s despite its simple
genome, and it is poorly understood which of them are actually working on collagen
in the fly. In this study, we addressed this question by carrying out thorough
bioinformatic and biochemical analyses. We demonstrate that among the 26 potential
collagen P4H⍺s, PH4⍺EFB shares the highest homology with vertebrate collagen P4H⍺s.
Furthermore, while collagen P4Hs and their substrates must exist in the same cells,
our transcriptomic analyses at the tissue and single cell levels showed a global
co-expression of PH4⍺EFB but not the other P4H⍺-related genes with the collagen
IV genes. Moreover, expression of PH4⍺EFB during embryogenesis was found to precede
that of collagen IV, presumably enabling efficient collagen modification by PH4⍺EFB.
Finally, biochemical assays confirm that PH4⍺EFB binds collagen, supporting its
direct role in collagen IV modification. Collectively, we identify PH4⍺EFB as
the primary and potentially constitutive prolyl 4-hydroxylase responsible for
collagen IV biosynthesis in Drosophila. Our findings highlight the remarkably
simple nature of Drosophila collagen IV biosynthesis, which may serve as a blueprint
for defining the minimal requirements for collagen engineering.
acknowledgement: "This project was supported by the All May See Foundation 7031,182
to YI, the Louisiana Board of Regents Support Fund: Research Competitiveness Subprogram
to MAT, Austrian science fund (FWF) as part of the SFB Meiosis consortium FWF SFB
F88-10 to Beatriz Vicoso (supported ME), American Heart Association 16POST2726018
and American Cancer Society 132,123-PF-18–025–01-CSM postdoctoral fellowships to
ALZ, National Institutes of Health R01 GM136961 and R35 GM148485 to SH-B, and the
Academy of Medical Sciences/the Wellcome Trust/ the Government Department of Business,
Energy and Industrial Strategy/the British Heart Foundation/Diabetes UK Springboard
Award SBF008\\1115 to YM. \r\nComputational analyses of single-nucleus transcriptome
data were performed on the high performance computer (HPC) at Bournemouth University,
the HPC at Institute of Science and Technology Austria, and the high-performance
computational resources provided by the Louisiana Optical Network Infrastructure
(http://www.loni.org). The authors are grateful to the researchers who published
the transcriptome datasets [48,49,52,55] that became the essential bases for this
study, to FlyBase for curating the datasets in an easily accessible format, and
the Drosophila Genomics Resource Center (DGRC), supported by NIH grant 2P40OD010949,
for providing the D17 cell line used in this research. The authors thank Kristian
Koski (University of Oulu, Finland) for crucial advice on the domain structure of
collagen P4H⍺s, and Ryusuke Niwa and Ryo Hoshino (University of Tsukuba, Japan)
for helpful discussions on SP."
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Yoshihiro
full_name: Ishikawa, Yoshihiro
last_name: Ishikawa
- first_name: Melissa A
full_name: Toups, Melissa A
id: 4E099E4E-F248-11E8-B48F-1D18A9856A87
last_name: Toups
orcid: 0000-0002-9752-7380
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Allison L.
full_name: Zajac, Allison L.
last_name: Zajac
- first_name: Sally
full_name: Horne-Badovinac, Sally
last_name: Horne-Badovinac
- first_name: Yutaka
full_name: Matsubayashi, Yutaka
last_name: Matsubayashi
citation:
ama: Ishikawa Y, Toups MA, Elkrewi MN, Zajac AL, Horne-Badovinac S, Matsubayashi
Y. Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation of Drosophila
collagen IV. Matrix Biology. 2025;141(11):101-113. doi:10.1016/j.matbio.2025.09.002
apa: Ishikawa, Y., Toups, M. A., Elkrewi, M. N., Zajac, A. L., Horne-Badovinac,
S., & Matsubayashi, Y. (2025). Evidence for the major role of PH4⍺EFB in the
prolyl 4-hydroxylation of Drosophila collagen IV. Matrix Biology. Springer
Nature. https://doi.org/10.1016/j.matbio.2025.09.002
chicago: Ishikawa, Yoshihiro, Melissa A Toups, Marwan N Elkrewi, Allison L. Zajac,
Sally Horne-Badovinac, and Yutaka Matsubayashi. “Evidence for the Major Role of
PH4⍺EFB in the Prolyl 4-Hydroxylation of Drosophila Collagen IV.” Matrix Biology.
Springer Nature, 2025. https://doi.org/10.1016/j.matbio.2025.09.002.
ieee: Y. Ishikawa, M. A. Toups, M. N. Elkrewi, A. L. Zajac, S. Horne-Badovinac,
and Y. Matsubayashi, “Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation
of Drosophila collagen IV,” Matrix Biology, vol. 141, no. 11. Springer
Nature, pp. 101–113, 2025.
ista: Ishikawa Y, Toups MA, Elkrewi MN, Zajac AL, Horne-Badovinac S, Matsubayashi
Y. 2025. Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation
of Drosophila collagen IV. Matrix Biology. 141(11), 101–113.
mla: Ishikawa, Yoshihiro, et al. “Evidence for the Major Role of PH4⍺EFB in the
Prolyl 4-Hydroxylation of Drosophila Collagen IV.” Matrix Biology, vol.
141, no. 11, Springer Nature, 2025, pp. 101–13, doi:10.1016/j.matbio.2025.09.002.
short: Y. Ishikawa, M.A. Toups, M.N. Elkrewi, A.L. Zajac, S. Horne-Badovinac, Y.
Matsubayashi, Matrix Biology 141 (2025) 101–113.
date_created: 2025-09-28T22:01:26Z
date_published: 2025-11-01T00:00:00Z
date_updated: 2026-01-05T13:09:08Z
day: '01'
ddc:
- '570'
department:
- _id: BeVi
doi: 10.1016/j.matbio.2025.09.002
external_id:
isi:
- '001583892100002'
pmid:
- '40946811'
file:
- access_level: open_access
checksum: 764257db41865d19daec1935788f72d7
content_type: application/pdf
creator: dernst
date_created: 2026-01-05T13:09:01Z
date_updated: 2026-01-05T13:09:01Z
file_id: '20948'
file_name: 2025_MatrixBiology_Ishikawa.pdf
file_size: 5844254
relation: main_file
success: 1
file_date_updated: 2026-01-05T13:09:01Z
has_accepted_license: '1'
intvolume: ' 141'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: 101-113
pmid: 1
project:
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
publication: Matrix Biology
publication_identifier:
eissn:
- 1569-1802
issn:
- 0945-053X
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evidence for the major role of PH4⍺EFB in the prolyl 4-hydroxylation of Drosophila
collagen IV
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 141
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20796'
abstract:
- lang: eng
text: Rapid prophase chromosome movements ensure faithful alignment of the parental
homologous chromosomes and successful synapsis formation during meiosis. These
movements are driven by cytoplasmic forces transmitted to the nuclear periphery,
where chromosome ends are attached through transmembrane proteins. During many
developmental stages a specific genome architecture with chromatin nuclear periphery
contacts mediates specific gene expression. Whether chromatin is removed from
the nuclear periphery as a consequence of chromosome motions or by a specific
mechanism is not fully understood. Here, we identify a mechanism to remove chromatin
from the nuclear periphery through vaccinia related kinase (VRK-1)–dependent phosphorylation
of Barrier to Autointegration Factor 1 (BAF-1) in Caenorhabditis elegans early
prophase of meiosis. Interfering with chromatin removal delays chromosome pairing,
impairs synapsis, produces oocytes with abnormal chromosomes and elevated apoptosis.
Long read sequencing reveals deletions and duplications in offspring lacking VRK-1
underscoring the importance of the BAF-1–VRK-1 module in preserving genome stability
in gametes during rapid chromosome movements.
acknowledgement: We are grateful to Monique Zetka, Nicola Silva, and Yumi Kim, Needhi
Bhalla, George Krohne and Rueyling Lin for providing reagents; Scott Kennedy for
sharing the multiplexed FISH library; and members of the Max Perutz Labs’ BioOptics
facility (Irmgard Fischer, Josef Gotzmann, Thomas Peterbauer, Clara Bodner, and
Nick Wedige) for training and support in image acquisition. We also thank the members
of the NGS facility at the Vienna Biocenter. This work was funded by the Austrian
Science Fund (FWF) SFB projects F 8805-B (VJ), https://doi.org/10.55776/F88, F 8809-B
(ITB), and F8810-B (BV). We are also grateful to members of the V. Jantsch laboratory
for helpful discussions. Some strains were provided by the Caenorhabditis Genetics
Center, which is funded by the National Institutes of Health Office of Research
Infrastructure Programs (P40OD010440).
article_number: '10446'
article_processing_charge: Yes
article_type: original
author:
- first_name: Dimitra
full_name: Paouneskou, Dimitra
last_name: Paouneskou
- first_name: Antoine
full_name: Baudrimont, Antoine
last_name: Baudrimont
- first_name: Réka K
full_name: Kelemen, Réka K
id: 48D3F8DE-F248-11E8-B48F-1D18A9856A87
last_name: Kelemen
orcid: 0000-0002-8489-9281
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Angela
full_name: Graf, Angela
last_name: Graf
- first_name: Shehab
full_name: Moukbel Ali Aldawla, Shehab
last_name: Moukbel Ali Aldawla
- first_name: Claudia
full_name: Kölbl, Claudia
last_name: Kölbl
- first_name: Irene
full_name: Tiemann-Boege, Irene
last_name: Tiemann-Boege
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
- first_name: Verena
full_name: Jantsch, Verena
last_name: Jantsch
citation:
ama: Paouneskou D, Baudrimont A, Kelemen RK, et al. BAF-1–VRK-1 mediated release
of meiotic chromosomes from the nuclear periphery is important for genome integrity.
Nature Communications. 2025;16. doi:10.1038/s41467-025-65420-9
apa: Paouneskou, D., Baudrimont, A., Kelemen, R. K., Elkrewi, M. N., Graf, A., Moukbel
Ali Aldawla, S., … Jantsch, V. (2025). BAF-1–VRK-1 mediated release of meiotic
chromosomes from the nuclear periphery is important for genome integrity. Nature
Communications. Springer Nature. https://doi.org/10.1038/s41467-025-65420-9
chicago: Paouneskou, Dimitra, Antoine Baudrimont, Réka K Kelemen, Marwan N Elkrewi,
Angela Graf, Shehab Moukbel Ali Aldawla, Claudia Kölbl, Irene Tiemann-Boege, Beatriz
Vicoso, and Verena Jantsch. “BAF-1–VRK-1 Mediated Release of Meiotic Chromosomes
from the Nuclear Periphery Is Important for Genome Integrity.” Nature Communications.
Springer Nature, 2025. https://doi.org/10.1038/s41467-025-65420-9.
ieee: D. Paouneskou et al., “BAF-1–VRK-1 mediated release of meiotic chromosomes
from the nuclear periphery is important for genome integrity,” Nature Communications,
vol. 16. Springer Nature, 2025.
ista: Paouneskou D, Baudrimont A, Kelemen RK, Elkrewi MN, Graf A, Moukbel Ali Aldawla
S, Kölbl C, Tiemann-Boege I, Vicoso B, Jantsch V. 2025. BAF-1–VRK-1 mediated release
of meiotic chromosomes from the nuclear periphery is important for genome integrity.
Nature Communications. 16, 10446.
mla: Paouneskou, Dimitra, et al. “BAF-1–VRK-1 Mediated Release of Meiotic Chromosomes
from the Nuclear Periphery Is Important for Genome Integrity.” Nature Communications,
vol. 16, 10446, Springer Nature, 2025, doi:10.1038/s41467-025-65420-9.
short: D. Paouneskou, A. Baudrimont, R.K. Kelemen, M.N. Elkrewi, A. Graf, S. Moukbel
Ali Aldawla, C. Kölbl, I. Tiemann-Boege, B. Vicoso, V. Jantsch, Nature Communications
16 (2025).
date_created: 2025-12-11T10:45:06Z
date_published: 2025-11-25T00:00:00Z
date_updated: 2025-12-15T09:28:37Z
day: '25'
ddc:
- '570'
department:
- _id: BeVi
doi: 10.1038/s41467-025-65420-9
external_id:
pmid:
- '41290579'
file:
- access_level: open_access
checksum: a952f7ea050242b79008540de49a0e61
content_type: application/pdf
creator: dernst
date_created: 2025-12-15T09:25:51Z
date_updated: 2025-12-15T09:25:51Z
file_id: '20823'
file_name: 2025_NatureComm_Paouneskou.pdf
file_size: 8096309
relation: main_file
success: 1
file_date_updated: 2025-12-15T09:25:51Z
has_accepted_license: '1'
intvolume: ' 16'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: BAF-1–VRK-1 mediated release of meiotic chromosomes from the nuclear periphery
is important for genome integrity
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2025'
...
---
OA_embargo: '12'
OA_place: publisher
_id: '19386'
abstract:
- lang: eng
text: "Crustaceans are a large group of arthropods with a great diversity of species
and\r\ndifferent types of sex determination systems and reproductive modes (Subramoniam,
2017).\r\nThis makes them a great model for exploring the evolution of sex chromosomes
and sexual\r\ndimorphism and investigating the evolutionary mechanisms driving
and maintaining the\r\ndiversity of reproductive systems. Within this taxon, Brine
shrimp of the genus Artemia, a\r\nbranchiopod crustacean, are well suited for
such explorations, as they have both highly\r\ndimorphic traits and closely related
sexual and asexual species. Although brine shrimp are\r\nknown to have ZW sex
chromosomes (Bowen, 1963; Parraguez et al., 2009), the sex\r\nchromosomes are
still not well characterized at the genomic level, the sex-determination gene\r\nis
unknown, and it is still unclear whether the same sex chromosomes as shared by
the\r\ndifferent species.\r\nThe first part of this thesis was to characterize
the Z and W chromosomes in Artemia\r\nusing an array of methods, from generating
multiple chromosome and contig level genome\r\nassemblies to identifying W-linked
scaffolds and transcripts in multiple species using k-mer\r\nbased approaches.\r\nThe
second part tackles the conservation of the cell type specific regulatory pathways\r\nin
the female reproductive system between Artemia and Drosophila, and the expression
of the\r\nZ-specific region throughout meiosis using single-nucleus RNA-seq data.
Our results show\r\nthat germline cells lack dosage compensation, with a subset
of cells showing evidence of\r\nextreme repression of the Z chromosome.\r\nWith
multiple sexual species and several asexual lineages of parthenogenetic females\r\nthat
produce rare males at low frequencies, Brine shrimp present the perfect opportunity
to\r\nexplore the transition to asexuality and shed light on the prerequisites
and repercussions of\r\nthe form of modified meiosis maintaining the asexual lineages.
The last chapter is an\r\ninvestigation of the molecular pathways involved in
asexual reproduction in Artemia using\r\nnewly generated single nucleus RNAseq
and WGS data and previously published data. "
acknowledged_ssus:
- _id: ScienComp
acknowledgement: My PhD work was funded by the Austrian science fund (FWF), as part
of the SFB Meiosis consortium (https://sfbmeiosis.org/, grant ID FWF SFB F88-10).
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
citation:
ama: Elkrewi MN. Evolution of sex chromosomes, sex determination and asexuality
in Artemia brine shrimp. 2025. doi:10.15479/AT-ISTA-19386
apa: Elkrewi, M. N. (2025). Evolution of sex chromosomes, sex determination and
asexuality in Artemia brine shrimp. Institute of Science and Technology Austria.
https://doi.org/10.15479/AT-ISTA-19386
chicago: Elkrewi, Marwan N. “Evolution of Sex Chromosomes, Sex Determination and
Asexuality in Artemia Brine Shrimp.” Institute of Science and Technology Austria,
2025. https://doi.org/10.15479/AT-ISTA-19386.
ieee: M. N. Elkrewi, “Evolution of sex chromosomes, sex determination and asexuality
in Artemia brine shrimp,” Institute of Science and Technology Austria, 2025.
ista: Elkrewi MN. 2025. Evolution of sex chromosomes, sex determination and asexuality
in Artemia brine shrimp. Institute of Science and Technology Austria.
mla: Elkrewi, Marwan N. Evolution of Sex Chromosomes, Sex Determination and Asexuality
in Artemia Brine Shrimp. Institute of Science and Technology Austria, 2025,
doi:10.15479/AT-ISTA-19386.
short: M.N. Elkrewi, Evolution of Sex Chromosomes, Sex Determination and Asexuality
in Artemia Brine Shrimp, Institute of Science and Technology Austria, 2025.
corr_author: '1'
date_created: 2025-03-11T12:54:31Z
date_published: 2025-03-14T00:00:00Z
date_updated: 2026-04-16T12:20:41Z
day: '14'
ddc:
- '570'
- '576'
degree_awarded: PhD
department:
- _id: GradSch
- _id: BeVi
doi: 10.15479/AT-ISTA-19386
file:
- access_level: closed
checksum: 5549a8216c07e4c39281648912d72246
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: melkrewi
date_created: 2025-03-26T07:06:56Z
date_updated: 2026-03-26T23:30:03Z
embargo_to: open_access
file_id: '19462'
file_name: Thesis_Marwan_Elkrewi.docx
file_size: 25019680
relation: source_file
- access_level: open_access
checksum: aed2ba9965aa89b3414deae1ae9f4321
content_type: application/pdf
creator: melkrewi
date_created: 2025-03-26T07:06:22Z
date_updated: 2026-03-26T23:30:03Z
embargo: 2026-03-26
file_id: '19463'
file_name: Thesis_Marwan_Elkrewi.pdf
file_size: 17294844
relation: main_file
file_date_updated: 2026-03-26T23:30:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '170'
project:
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
publication_identifier:
eissn:
- 2663-337X
isbn:
- '9783990780534'
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '12248'
relation: part_of_dissertation
status: public
- id: '10167'
relation: part_of_dissertation
status: public
- id: '10767'
relation: part_of_dissertation
status: public
- id: '15009'
relation: part_of_dissertation
status: public
- id: '14613'
relation: part_of_dissertation
status: public
- id: '17890'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
title: Evolution of sex chromosomes, sex determination and asexuality in Artemia brine
shrimp
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2025'
...
---
_id: '14705'
abstract:
- lang: eng
text: Since the commercialization of brine shrimp (genus Artemia) in the 1950s,
this lineage, and in particular the model species Artemia franciscana, has been
the subject of extensive research. However, our understanding of the genetic mechanisms
underlying various aspects of their reproductive biology, including sex determination,
are still lacking. This is partly due to the scarcity of genomic resources for
Artemia species and crustaceans in general. Here, we present a chromosome-level
genome assembly of Artemia franciscana (Kellogg 1906), from the Great Salt Lake,
USA. The genome is 1GB, and the majority of the genome (81%) is scaffolded into
21 linkage groups using a previously published high-density linkage map. We performed
coverage and FST analyses using male and female genomic and transcriptomic reads
to quantify the extent of differentiation between the Z and W chromosomes. Additionally,
we quantified the expression levels in male and female heads and gonads and found
further evidence for dosage compensation in this species.
article_processing_charge: No
author:
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
citation:
ama: Elkrewi MN. Data from “Chromosome-level assembly of Artemia franciscana sheds
light on sex-chromosome differentiation.” 2024. doi:10.15479/AT:ISTA:14705
apa: Elkrewi, M. N. (2024). Data from “Chromosome-level assembly of Artemia franciscana
sheds light on sex-chromosome differentiation.” Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:14705
chicago: Elkrewi, Marwan N. “Data from ‘Chromosome-Level Assembly of Artemia Franciscana
Sheds Light on Sex-Chromosome Differentiation.’” Institute of Science and Technology
Austria, 2024. https://doi.org/10.15479/AT:ISTA:14705.
ieee: M. N. Elkrewi, “Data from ‘Chromosome-level assembly of Artemia franciscana
sheds light on sex-chromosome differentiation.’” Institute of Science and Technology
Austria, 2024.
ista: Elkrewi MN. 2024. Data from ‘Chromosome-level assembly of Artemia franciscana
sheds light on sex-chromosome differentiation’, Institute of Science and Technology
Austria, 10.15479/AT:ISTA:14705.
mla: Elkrewi, Marwan N. Data from “Chromosome-Level Assembly of Artemia Franciscana
Sheds Light on Sex-Chromosome Differentiation.” Institute of Science and Technology
Austria, 2024, doi:10.15479/AT:ISTA:14705.
short: M.N. Elkrewi, (2024).
contributor:
- contributor_type: researcher
first_name: Vincent K
id: 57854184-AAE0-11E9-8D04-98D6E5697425
last_name: Bett
- contributor_type: project_member
first_name: Ariana
id: 2A0848E2-F248-11E8-B48F-1D18A9856A87
last_name: Macon
- contributor_type: supervisor
first_name: Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
- contributor_type: researcher
first_name: Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
corr_author: '1'
date_created: 2023-12-22T13:40:48Z
date_published: 2024-01-02T00:00:00Z
date_updated: 2025-09-04T12:05:42Z
day: '02'
ddc:
- '576'
department:
- _id: GradSch
- _id: BeVi
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content_type: text/plain
creator: melkrewi
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content_type: application/x-zip-compressed
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date_updated: 2023-12-22T14:14:06Z
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file_size: 343632753
relation: main_file
success: 1
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keyword:
- sex chromosome evolution
- genome assembly
- dosage compensation
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
publisher: Institute of Science and Technology Austria
related_material:
record:
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relation: used_in_publication
status: public
status: public
title: Data from "Chromosome-level assembly of Artemia franciscana sheds light on
sex-chromosome differentiation"
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '17362'
abstract:
- lang: eng
text: This is the supplementary data for the paper titled "Single-nucleus atlas
of the Artemia female reproductive system suggests germline repression of the
Z chromosome", where we described the generation and analysis of single-nucleus
expression and chromatin-accessibility data from the female reproductive system
of Artemia franciscana. We compared our dataset to the published Drosophila single-nucleus
data (over 400 million years of divergence) and highlighted the extreme conservation
of several of the molecular pathways of oogenesis and meiosis. We found evidence
of global transcriptional quiescence and chromatin condensation in late germ cells,
highlighting the conserved role of this repressive stage in arthropod oogenesis.
Additionally, we explored the expression patterns of the ZW sex chromosomes during
oogenesis. Our data shows that the Z-chromosome is consistently downregulated
in germline cells. While this is partly driven by a lack of dosage compensation
in the germline, a subset of cells show stronger repression of the Z chromosome.
acknowledged_ssus:
- _id: ScienComp
article_processing_charge: No
author:
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
citation:
ama: 'Elkrewi MN, Vicoso B. Data for: “Single-nucleus atlas of the Artemia female
reproductive system suggests germline repression of the Z chromosome.” 2024. doi:10.15479/AT:ISTA:17362'
apa: 'Elkrewi, M. N., & Vicoso, B. (2024). Data for: “Single-nucleus atlas of
the Artemia female reproductive system suggests germline repression of the Z chromosome.”
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:17362'
chicago: 'Elkrewi, Marwan N, and Beatriz Vicoso. “Data for: ‘Single-Nucleus Atlas
of the Artemia Female Reproductive System Suggests Germline Repression of the
Z Chromosome.’” Institute of Science and Technology Austria, 2024. https://doi.org/10.15479/AT:ISTA:17362.'
ieee: 'M. N. Elkrewi and B. Vicoso, “Data for: ‘Single-nucleus atlas of the Artemia
female reproductive system suggests germline repression of the Z chromosome.’”
Institute of Science and Technology Austria, 2024.'
ista: 'Elkrewi MN, Vicoso B. 2024. Data for: ‘Single-nucleus atlas of the Artemia
female reproductive system suggests germline repression of the Z chromosome’,
Institute of Science and Technology Austria, 10.15479/AT:ISTA:17362.'
mla: 'Elkrewi, Marwan N., and Beatriz Vicoso. Data for: “Single-Nucleus Atlas
of the Artemia Female Reproductive System Suggests Germline Repression of the
Z Chromosome.” Institute of Science and Technology Austria, 2024, doi:10.15479/AT:ISTA:17362.'
short: M.N. Elkrewi, B. Vicoso, (2024).
corr_author: '1'
date_created: 2024-08-02T07:27:45Z
date_published: 2024-08-05T00:00:00Z
date_updated: 2026-04-16T12:20:41Z
day: '05'
ddc:
- '576'
department:
- _id: GradSch
- _id: BeVi
doi: 10.15479/AT:ISTA:17362
file:
- access_level: open_access
checksum: 26b5d41b3103f4284dd97d56e370a5b6
content_type: text/plain
creator: melkrewi
date_created: 2024-08-05T22:24:18Z
date_updated: 2024-08-05T22:24:18Z
file_id: '17394'
file_name: README.txt
file_size: 2465
relation: main_file
success: 1
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checksum: 95adab5e36148015da313505e3910707
content_type: application/x-zip-compressed
creator: melkrewi
date_created: 2024-08-05T23:28:52Z
date_updated: 2024-08-05T23:28:52Z
file_id: '17395'
file_name: Data_artemia_single_nucleus_atlas.zip
file_size: 2526735400
relation: main_file
success: 1
file_date_updated: 2024-08-05T23:28:52Z
has_accepted_license: '1'
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '17890'
relation: used_in_publication
status: public
status: public
title: 'Data for: "Single-nucleus atlas of the Artemia female reproductive system
suggests germline repression of the Z chromosome"'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
APC_amount: 3145,39 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '17890'
abstract:
- lang: eng
text: Our understanding of the molecular pathways that regulate oogenesis and define
cellular identity in the Arthropod female reproductive system and the extent of
their conservation is currently very limited. This is due to the focus on model
systems, including Drosophila and Daphnia, which do not reflect the observed diversity
of morphologies, reproductive modes, and sex chromosome systems. We use single-nucleus
RNA and ATAC sequencing to produce a comprehensive single nucleus atlas of the
adult Artemia franciscana female reproductive system. We map our data to the Fly
Cell Atlas single-nucleus dataset of the Drosophila melanogaster ovary, shedding
light on the conserved regulatory programs between the two distantly related Arthropod
species. We identify the major cell types known to be present in the Artemia ovary,
including germ cells, follicle cells, and ovarian muscle cells. Additionally,
we use the germ cells to explore gene regulation and expression of the Z chromosome
during meiosis, highlighting its unique regulatory dynamics and allowing us to
explore the presence of meiotic sex chromosome silencing in this group.
acknowledged_ssus:
- _id: ScienComp
acknowledgement: "We thank the Vicoso group for their valuable comments on the earlier
draft of the manuscript. We would also like to thank the Vienna BioCenter Next Generation
Sequencing (NGS) facility staff, and in particular, Thomas Grentzinger for his support
with the handling and sequencing of the samples, the scientific computing unit at
ISTA for the computational resources, Brittney Wick for the help with hosting our
data on the UCSC Cell Browser, and Lora B. Sweeney for her valuable input at the
different stages of the project.\r\nThis research was funded by the Austrian science
fund (FWF), as part of the SFB Meiosis consortium https://sfbmeiosis.org/, grant
ID FWF SFB F88-10) to BV. "
article_number: e1011376
article_processing_charge: Yes
article_type: original
author:
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
citation:
ama: Elkrewi MN, Vicoso B. Single-nucleus atlas of the Artemia female reproductive
system suggests germline repression of the Z chromosome. PLoS Genetics.
2024;20(8). doi:10.1371/journal.pgen.1011376
apa: Elkrewi, M. N., & Vicoso, B. (2024). Single-nucleus atlas of the Artemia
female reproductive system suggests germline repression of the Z chromosome. PLoS
Genetics. Public Library of Science. https://doi.org/10.1371/journal.pgen.1011376
chicago: Elkrewi, Marwan N, and Beatriz Vicoso. “Single-Nucleus Atlas of the Artemia
Female Reproductive System Suggests Germline Repression of the Z Chromosome.”
PLoS Genetics. Public Library of Science, 2024. https://doi.org/10.1371/journal.pgen.1011376.
ieee: M. N. Elkrewi and B. Vicoso, “Single-nucleus atlas of the Artemia female reproductive
system suggests germline repression of the Z chromosome,” PLoS Genetics,
vol. 20, no. 8. Public Library of Science, 2024.
ista: Elkrewi MN, Vicoso B. 2024. Single-nucleus atlas of the Artemia female reproductive
system suggests germline repression of the Z chromosome. PLoS Genetics. 20(8),
e1011376.
mla: Elkrewi, Marwan N., and Beatriz Vicoso. “Single-Nucleus Atlas of the Artemia
Female Reproductive System Suggests Germline Repression of the Z Chromosome.”
PLoS Genetics, vol. 20, no. 8, e1011376, Public Library of Science, 2024,
doi:10.1371/journal.pgen.1011376.
short: M.N. Elkrewi, B. Vicoso, PLoS Genetics 20 (2024).
corr_author: '1'
date_created: 2024-09-08T22:01:11Z
date_published: 2024-08-30T00:00:00Z
date_updated: 2026-06-15T22:31:14Z
day: '30'
ddc:
- '570'
department:
- _id: BeVi
doi: 10.1371/journal.pgen.1011376
external_id:
isi:
- '001304090200001'
pmid:
- '39213449'
file:
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checksum: f5d96b9af57126fc1063e951440477d6
content_type: application/pdf
creator: dernst
date_created: 2024-09-11T07:54:12Z
date_updated: 2024-09-11T07:54:12Z
file_id: '18056'
file_name: 2024_PloSGenetics_Elkrewi.pdf
file_size: 8962687
relation: main_file
success: 1
file_date_updated: 2024-09-11T07:54:12Z
has_accepted_license: '1'
intvolume: ' 20'
isi: 1
issue: '8'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 3AC91DDA-15DF-11EA-824D-93A3E7B544D1
call_identifier: FWF
name: FWF Open Access Fund
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
publication: PLoS Genetics
publication_identifier:
eissn:
- 1553-7404
issn:
- 1553-7390
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/Melkrewi/Artemia-snRNAseq-Project
record:
- id: '17362'
relation: research_data
status: public
- id: '19386'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Single-nucleus atlas of the Artemia female reproductive system suggests germline
repression of the Z chromosome
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 20
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '15009'
abstract:
- lang: eng
text: Since the commercialization of brine shrimp (genus Artemia) in the 1950s,
this lineage, and in particular the model species Artemia franciscana, has been
the subject of extensive research. However, our understanding of the genetic mechanisms
underlying various aspects of their reproductive biology, including sex determination,
is still lacking. This is partly due to the scarcity of genomic resources for
Artemia species and crustaceans in general. Here, we present a chromosome-level
genome assembly of A. franciscana (Kellogg 1906), from the Great Salt Lake, United
States. The genome is 1 GB, and the majority of the genome (81%) is scaffolded
into 21 linkage groups using a previously published high-density linkage map.
We performed coverage and FST analyses using male and female genomic and transcriptomic
reads to quantify the extent of differentiation between the Z and W chromosomes.
Additionally, we quantified the expression levels in male and female heads and
gonads and found further evidence for dosage compensation in this species.
article_number: evae006
article_processing_charge: Yes
article_type: original
author:
- first_name: Vincent K
full_name: Bett, Vincent K
id: 57854184-AAE0-11E9-8D04-98D6E5697425
last_name: Bett
- first_name: Ariana
full_name: Macon, Ariana
id: 2A0848E2-F248-11E8-B48F-1D18A9856A87
last_name: Macon
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
citation:
ama: Bett VK, Macon A, Vicoso B, Elkrewi MN. Chromosome-level assembly of Artemia
franciscana sheds light on sex chromosome differentiation. Genome Biology and
Evolution. 2024;16(1). doi:10.1093/gbe/evae006
apa: Bett, V. K., Macon, A., Vicoso, B., & Elkrewi, M. N. (2024). Chromosome-level
assembly of Artemia franciscana sheds light on sex chromosome differentiation.
Genome Biology and Evolution. Oxford University Press. https://doi.org/10.1093/gbe/evae006
chicago: Bett, Vincent K, Ariana Macon, Beatriz Vicoso, and Marwan N Elkrewi. “Chromosome-Level
Assembly of Artemia Franciscana Sheds Light on Sex Chromosome Differentiation.”
Genome Biology and Evolution. Oxford University Press, 2024. https://doi.org/10.1093/gbe/evae006.
ieee: V. K. Bett, A. Macon, B. Vicoso, and M. N. Elkrewi, “Chromosome-level assembly
of Artemia franciscana sheds light on sex chromosome differentiation,” Genome
Biology and Evolution, vol. 16, no. 1. Oxford University Press, 2024.
ista: Bett VK, Macon A, Vicoso B, Elkrewi MN. 2024. Chromosome-level assembly of
Artemia franciscana sheds light on sex chromosome differentiation. Genome Biology
and Evolution. 16(1), evae006.
mla: Bett, Vincent K., et al. “Chromosome-Level Assembly of Artemia Franciscana
Sheds Light on Sex Chromosome Differentiation.” Genome Biology and Evolution,
vol. 16, no. 1, evae006, Oxford University Press, 2024, doi:10.1093/gbe/evae006.
short: V.K. Bett, A. Macon, B. Vicoso, M.N. Elkrewi, Genome Biology and Evolution
16 (2024).
corr_author: '1'
date_created: 2024-02-18T23:01:02Z
date_published: 2024-01-20T00:00:00Z
date_updated: 2026-06-15T22:31:20Z
day: '20'
ddc:
- '570'
department:
- _id: BeVi
doi: 10.1093/gbe/evae006
external_id:
isi:
- '001153952800001'
pmid:
- '38245839'
file:
- access_level: open_access
checksum: 106a40f10443b2e7ba66749844ebbdf1
content_type: application/pdf
creator: dernst
date_created: 2024-02-26T09:54:59Z
date_updated: 2024-02-26T09:54:59Z
file_id: '15029'
file_name: 2024_GBE_Bett.pdf
file_size: 5213306
relation: main_file
success: 1
file_date_updated: 2024-02-26T09:54:59Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Genome Biology and Evolution
publication_identifier:
eissn:
- 1759-6653
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
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relation: research_data
status: public
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relation: dissertation_contains
status: deleted
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relation: dissertation_contains
status: public
- id: '20449'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Chromosome-level assembly of Artemia franciscana sheds light on sex chromosome
differentiation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 16
year: '2024'
...
---
_id: '14614'
abstract:
- lang: eng
text: 'Many insects carry an ancient X chromosome—the Drosophila Muller element
F—that likely predates their origin. Interestingly, the X has undergone turnover
in multiple fly species (Diptera) after being conserved for more than 450 My.
The long evolutionary distance between Diptera and other sequenced insect clades
makes it difficult to infer what could have contributed to this sudden increase
in rate of turnover. Here, we produce the first genome and transcriptome of scorpionflies
(genus Panorpa), an insect belonging to a long overlooked sister-order to Diptera:
Mecoptera. Combining our genome assembly with genomic short-read data, we obtain
genome coverage and identify X-linked super-scaffolds. We further perform a gene
homology analysis between the Panorpa X and a closely related Diptera species,
and we assess the conservation of the Panorpa X-linked gene content with that
of more distantly related insect species. We explored the structure of the Panorpa
X by determining its repeat content, GC content, and nucleotide diversity. Finally,
we used RNAseq data to detect the presence of dosage compensation in somatic tissues,
as well as to explore gene expression tissue-specificity, and sex-bias in gene
expression. We find high conservation of gene content between the mecopteran X
and the dipteran Muller F element, as well as several shared biological features,
such as the presence of dosage compensation and a low amount of genetic diversity,
consistent with a low recombination rate. However, the 2 homologous X chromosomes
differ strikingly in their size and number of genes they carry. Our results therefore
support a common ancestry of the mecopteran and ancestral dipteran X chromosomes,
and suggest that Muller element F shrank in size and gene content after the split
of Diptera and Mecoptera, which may have contributed to its turnover in dipteran
insects.'
article_processing_charge: No
author:
- first_name: Clementine
full_name: Lasne, Clementine
id: 02225f57-50d2-11eb-9ed8-8c92b9a34237
last_name: Lasne
orcid: 0000-0002-1197-8616
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
citation:
ama: Lasne C, Elkrewi MN. The scorpionfly (Panorpa cognata) genome highlights conserved
and derived features of the peculiar dipteran X chromosome. 2023. doi:10.15479/AT:ISTA:14614
apa: Lasne, C., & Elkrewi, M. N. (2023). The scorpionfly (Panorpa cognata) genome
highlights conserved and derived features of the peculiar dipteran X chromosome.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:14614
chicago: Lasne, Clementine, and Marwan N Elkrewi. “The Scorpionfly (Panorpa Cognata)
Genome Highlights Conserved and Derived Features of the Peculiar Dipteran X Chromosome.”
Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/AT:ISTA:14614.
ieee: C. Lasne and M. N. Elkrewi, “The scorpionfly (Panorpa cognata) genome highlights
conserved and derived features of the peculiar dipteran X chromosome.” Institute
of Science and Technology Austria, 2023.
ista: Lasne C, Elkrewi MN. 2023. The scorpionfly (Panorpa cognata) genome highlights
conserved and derived features of the peculiar dipteran X chromosome, Institute
of Science and Technology Austria, 10.15479/AT:ISTA:14614.
mla: Lasne, Clementine, and Marwan N. Elkrewi. The Scorpionfly (Panorpa Cognata)
Genome Highlights Conserved and Derived Features of the Peculiar Dipteran X Chromosome.
Institute of Science and Technology Austria, 2023, doi:10.15479/AT:ISTA:14614.
short: C. Lasne, M.N. Elkrewi, (2023).
contributor:
- contributor_type: researcher
first_name: Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
corr_author: '1'
date_created: 2023-11-27T16:39:19Z
date_published: 2023-12-01T00:00:00Z
date_updated: 2025-09-09T13:33:22Z
day: '01'
ddc:
- '576'
department:
- _id: BeVi
doi: 10.15479/AT:ISTA:14614
file:
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checksum: cd0f13322b5156819ecaebd2bc8e7d12
content_type: application/zip
creator: clasne
date_created: 2023-11-28T13:15:26Z
date_updated: 2023-11-28T13:15:26Z
file_id: '14625'
file_name: panorpaX.zip
file_size: 404968272
relation: main_file
success: 1
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checksum: 9ff600416577687a737cb3c96dfcb26c
content_type: text/plain
creator: clasne
date_created: 2023-11-30T14:16:59Z
date_updated: 2023-11-30T14:16:59Z
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file_name: panorpa_readme.txt
file_size: 2625
relation: main_file
success: 1
file_date_updated: 2023-11-30T14:16:59Z
has_accepted_license: '1'
keyword:
- Panorpa
- scorpionfly
- genome
- transcriptome
month: '12'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '14613'
relation: used_in_publication
status: public
status: public
title: The scorpionfly (Panorpa cognata) genome highlights conserved and derived features
of the peculiar dipteran X chromosome
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '14613'
abstract:
- lang: eng
text: 'Many insects carry an ancient X chromosome - the Drosophila Muller element
F - that likely predates their origin. Interestingly, the X has undergone turnover
in multiple fly species (Diptera) after being conserved for more than 450 MY.
The long evolutionary distance between Diptera and other sequenced insect clades
makes it difficult to infer what could have contributed to this sudden increase
in rate of turnover. Here, we produce the first genome and transcriptome of a
long overlooked sister-order to Diptera: Mecoptera. We compare the scorpionfly
Panorpa cognata X-chromosome gene content, expression, and structure, to that
of several dipteran species as well as more distantly-related insect orders (Orthoptera
and Blattodea). We find high conservation of gene content between the mecopteran
X and the dipteran Muller F element, as well as several shared biological features,
such as the presence of dosage compensation and a low amount of genetic diversity,
consistent with a low recombination rate. However, the two homologous X chromosomes
differ strikingly in their size and number of genes they carry. Our results therefore
support a common ancestry of the mecopteran and ancestral dipteran X chromosomes,
and suggest that Muller element F shrank in size and gene content after the split
of Diptera and Mecoptera, which may have contributed to its turnover in dipteran
insects.'
acknowledged_ssus:
- _id: ScienComp
acknowledgement: "We thank the Vicoso lab for their assistance with specimen collection,
and Tim Connallon for valuable comments and suggestions on earlier versions of the
manuscript. Computational resources and support were provided by the Scientific
Computing unit at the ISTA. This research was supported by grants from the Austrian
Science Foundation to C.L.\r\n(FWF ESP 39), and to B.V. (FWF SFB F88-10)."
article_number: msad245
article_processing_charge: Yes
article_type: original
author:
- first_name: Clementine
full_name: Lasne, Clementine
id: 02225f57-50d2-11eb-9ed8-8c92b9a34237
last_name: Lasne
orcid: 0000-0002-1197-8616
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Melissa A
full_name: Toups, Melissa A
id: 4E099E4E-F248-11E8-B48F-1D18A9856A87
last_name: Toups
orcid: 0000-0002-9752-7380
- first_name: Lorena Alexandra
full_name: Layana Franco, Lorena Alexandra
id: 02814589-eb8f-11eb-b029-a70074f3f18f
last_name: Layana Franco
orcid: 0000-0002-1253-6297
- first_name: Ariana
full_name: Macon, Ariana
id: 2A0848E2-F248-11E8-B48F-1D18A9856A87
last_name: Macon
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
citation:
ama: Lasne C, Elkrewi MN, Toups MA, Layana Franco LA, Macon A, Vicoso B. The scorpionfly
(Panorpa cognata) genome highlights conserved and derived features of the peculiar
dipteran X chromosome. Molecular Biology and Evolution. 2023;40(12). doi:10.1093/molbev/msad245
apa: Lasne, C., Elkrewi, M. N., Toups, M. A., Layana Franco, L. A., Macon, A., &
Vicoso, B. (2023). The scorpionfly (Panorpa cognata) genome highlights conserved
and derived features of the peculiar dipteran X chromosome. Molecular Biology
and Evolution. Oxford University Press. https://doi.org/10.1093/molbev/msad245
chicago: Lasne, Clementine, Marwan N Elkrewi, Melissa A Toups, Lorena Alexandra
Layana Franco, Ariana Macon, and Beatriz Vicoso. “The Scorpionfly (Panorpa Cognata)
Genome Highlights Conserved and Derived Features of the Peculiar Dipteran X Chromosome.”
Molecular Biology and Evolution. Oxford University Press, 2023. https://doi.org/10.1093/molbev/msad245.
ieee: C. Lasne, M. N. Elkrewi, M. A. Toups, L. A. Layana Franco, A. Macon, and B.
Vicoso, “The scorpionfly (Panorpa cognata) genome highlights conserved and derived
features of the peculiar dipteran X chromosome,” Molecular Biology and Evolution,
vol. 40, no. 12. Oxford University Press, 2023.
ista: Lasne C, Elkrewi MN, Toups MA, Layana Franco LA, Macon A, Vicoso B. 2023.
The scorpionfly (Panorpa cognata) genome highlights conserved and derived features
of the peculiar dipteran X chromosome. Molecular Biology and Evolution. 40(12),
msad245.
mla: Lasne, Clementine, et al. “The Scorpionfly (Panorpa Cognata) Genome Highlights
Conserved and Derived Features of the Peculiar Dipteran X Chromosome.” Molecular
Biology and Evolution, vol. 40, no. 12, msad245, Oxford University Press,
2023, doi:10.1093/molbev/msad245.
short: C. Lasne, M.N. Elkrewi, M.A. Toups, L.A. Layana Franco, A. Macon, B. Vicoso,
Molecular Biology and Evolution 40 (2023).
corr_author: '1'
date_created: 2023-11-27T16:14:37Z
date_published: 2023-12-01T00:00:00Z
date_updated: 2026-06-15T22:31:14Z
day: '01'
ddc:
- '570'
department:
- _id: BeVi
doi: 10.1093/molbev/msad245
external_id:
isi:
- '001122489000003'
pmid:
- '37988296'
file:
- access_level: open_access
checksum: 47c1c72fb499f26ea52d216b242208c8
content_type: application/pdf
creator: dernst
date_created: 2024-01-02T11:39:38Z
date_updated: 2024-01-02T11:39:38Z
file_id: '14727'
file_name: 2023_MolecularBioEvo_Lasne.pdf
file_size: 8623505
relation: main_file
success: 1
file_date_updated: 2024-01-02T11:39:38Z
has_accepted_license: '1'
intvolume: ' 40'
isi: 1
issue: '12'
keyword:
- Genetics
- Molecular Biology
- Ecology
- Evolution
- Behavior and Systematics
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
- _id: ebb230e0-77a9-11ec-83b8-87a37e0241d3
grant_number: ESP39 49461
name: Mechanisms and Evolution of Reproductive Plasticity
publication: Molecular Biology and Evolution
publication_identifier:
eissn:
- 1537-1719
issn:
- 0737-4038
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
link:
- description: News on ISTA webpage
relation: press_release
url: https://ista.ac.at/en/news/on-the-hunt/
record:
- id: '14614'
relation: research_data
status: public
- id: '19386'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: The scorpionfly (Panorpa cognata) genome highlights conserved and derived features
of the peculiar dipteran X chromosome
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 40
year: '2023'
...
---
_id: '11653'
abstract:
- lang: eng
text: Eurasian brine shrimp (genus Artemia) have closely related sexual and asexual
lineages of parthenogenetic females, which produce rare males at low frequencies.
Although they are known to have ZW chromosomes, these are not well characterized,
and it is unclear whether they are shared across the clade. Furthermore, the underlying
genetic architecture of the transmission of asexuality, which can occur when rare
males mate with closely related sexual females, is not well understood. We produced
a chromosome-level assembly for the sexual Eurasian species A. sinica and characterized
in detail the pair of sex chromosomes of this species. We combined this new assembly
with short-read genomic data for the sexual species A. sp. Kazakhstan and several
asexual lineages of A. parthenogenetica, allowing us to perform an in-depth characterization
of sex-chromosome evolution across the genus. We identified a small differentiated
region of the ZW pair that is shared by all sexual and asexual lineages, supporting
the shared ancestry of the sex chromosomes. We also inferred that recombination
suppression has spread to larger sections of the chromosome independently in the
American and Eurasian lineages. Finally, we took advantage of a rare male, which
we backcrossed to sexual females, to explore the genetic basis of asexuality.
Our results suggest that parthenogenesis is likely partly controlled by a locus
on the Z chromosome, highlighting the interplay between sex determination and
asexuality.
article_processing_charge: No
author:
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
citation:
ama: Elkrewi MN. Data from Elkrewi, Khauratovich, Toups et al. 2022, “ZW sex-chromosome
evolution and contagious parthenogenesis in Artemia brine shrimp.” 2022. doi:10.15479/AT:ISTA:11653
apa: Elkrewi, M. N. (2022). Data from Elkrewi, Khauratovich, Toups et al. 2022,
“ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp.”
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:11653
chicago: Elkrewi, Marwan N. “Data from Elkrewi, Khauratovich, Toups et Al. 2022,
‘ZW Sex-Chromosome Evolution and Contagious Parthenogenesis in Artemia Brine Shrimp.’”
Institute of Science and Technology Austria, 2022. https://doi.org/10.15479/AT:ISTA:11653.
ieee: M. N. Elkrewi, “Data from Elkrewi, Khauratovich, Toups et al. 2022, ‘ZW sex-chromosome
evolution and contagious parthenogenesis in Artemia brine shrimp.’” Institute
of Science and Technology Austria, 2022.
ista: Elkrewi MN. 2022. Data from Elkrewi, Khauratovich, Toups et al. 2022, ‘ZW
sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp’,
Institute of Science and Technology Austria, 10.15479/AT:ISTA:11653.
mla: Elkrewi, Marwan N. Data from Elkrewi, Khauratovich, Toups et Al. 2022, “ZW
Sex-Chromosome Evolution and Contagious Parthenogenesis in Artemia Brine Shrimp.”
Institute of Science and Technology Austria, 2022, doi:10.15479/AT:ISTA:11653.
short: M.N. Elkrewi, (2022).
contributor:
- first_name: Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Uladzislava
last_name: Khauratovich
- first_name: Melissa A
id: 4E099E4E-F248-11E8-B48F-1D18A9856A87
last_name: Toups
- first_name: Vincent K
id: 57854184-AAE0-11E9-8D04-98D6E5697425
last_name: Bett
- first_name: Andrea
id: 353FAC84-AE61-11E9-8BFC-00D3E5697425
last_name: Mrnjavac
- first_name: Ariana
id: 2A0848E2-F248-11E8-B48F-1D18A9856A87
last_name: Macon
- first_name: Christelle
id: 32DF5794-F248-11E8-B48F-1D18A9856A87
last_name: Fraisse
orcid: 0000-0001-8441-5075
- first_name: Luca
last_name: Sax
- first_name: Ann K
id: 4C0A3874-F248-11E8-B48F-1D18A9856A87
last_name: Huylmans
- first_name: Francisco
last_name: 'Hontoria '
- first_name: Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
corr_author: '1'
date_created: 2022-07-26T11:01:47Z
date_published: 2022-08-05T00:00:00Z
date_updated: 2025-04-15T08:34:17Z
day: '05'
ddc:
- '570'
department:
- _id: GradSch
- _id: BeVi
doi: 10.15479/AT:ISTA:11653
file:
- access_level: open_access
checksum: 5f1d7c6d7ab5375ed2564521432bed0c
content_type: application/x-zip-compressed
creator: melkrewi
date_created: 2022-07-26T12:37:52Z
date_updated: 2022-08-08T22:30:04Z
description: |
The folder contains the following datasets (fasta files, and text files):
Sup. Dataset 1: Genome assemblies: A. sinica male high quality assembly, A. sp. Kazakhstan
male draft assembly
Sup. Dataset 2: Male transcriptome assemblies for A. sinica and A. franciscana
Sup. Dataset 3: Male and female coverage for A. sinica, A. sp. Kazakhstan, A. urmiana, and
A. parthenogenetica females and rare male.
Sup. Dataset 4: Artemia sinica Male:female FST per 1Kb window
Sup. Dataset 5: FASTA file with candidate W scaffolds
Sup. Dataset 6: Candidate W-derived transcripts and alignments
Sup. Dataset 7: Gene expression with genomic location
Sup. Dataset 8: VCF for asexual female and rare male
Sup. Dataset 9: FST between backcrossed asexual and control females (pooled analysis)
Sup. Dataset 10: VCF of backcrossed asexual and control females (individual analysis using
A. sp. Kazakhstan as the reference), and inferred ancestry
Sup. Dataset 11: GO and DE annotations of all the Artemia sinica transcripts and their
locations in the Artemia sinica male genome.
embargo: 2022-08-07
file_id: '11655'
file_name: Data.zip
file_size: 2209382998
relation: main_file
title: Supplementary Datasets
file_date_updated: 2022-08-08T22:30:04Z
has_accepted_license: '1'
month: '08'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '12248'
relation: used_in_publication
status: public
status: public
title: Data from Elkrewi, Khauratovich, Toups et al. 2022, "ZW sex-chromosome evolution
and contagious parthenogenesis in Artemia brine shrimp"
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2022'
...
---
_id: '12248'
abstract:
- lang: eng
text: Eurasian brine shrimp (genus Artemia) have closely related sexual and asexual
lineages of parthenogenetic females, which produce rare males at low frequencies.
Although they are known to have ZW chromosomes, these are not well characterized,
and it is unclear whether they are shared across the clade. Furthermore, the underlying
genetic architecture of the transmission of asexuality, which can occur when rare
males mate with closely related sexual females, is not well understood. We produced
a chromosome-level assembly for the sexual Eurasian species Artemia sinica and
characterized in detail the pair of sex chromosomes of this species. We combined
this new assembly with short-read genomic data for the sexual species Artemia
sp. Kazakhstan and several asexual lineages of Artemia parthenogenetica, allowing
us to perform an in-depth characterization of sex-chromosome evolution across
the genus. We identified a small differentiated region of the ZW pair that is
shared by all sexual and asexual lineages, supporting the shared ancestry of the
sex chromosomes. We also inferred that recombination suppression has spread to
larger sections of the chromosome independently in the American and Eurasian lineages.
Finally, we took advantage of a rare male, which we backcrossed to sexual females,
to explore the genetic basis of asexuality. Our results suggest that parthenogenesis
is likely partly controlled by a locus on the Z chromosome, highlighting the interplay
between sex determination and asexuality.
acknowledged_ssus:
- _id: ScienComp
acknowledgement: "This work was supported by the European Research Council under the
European Union’s Horizon 2020 research and innovation program (grant agreement no.
715257) and by the Austrian Science Foundation (FWF SFB F88-10).\r\nWe thank the
Vicoso group for comments on the manuscript and the ISTA Scientific computing team
and the Vienna Biocenter Sequencing facility for technical support."
article_number: iyac123
article_processing_charge: No
article_type: original
author:
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Uladzislava
full_name: Khauratovich, Uladzislava
id: 5eba06f4-97d8-11ed-9f8f-d826ebdd9434
last_name: Khauratovich
- first_name: Melissa A
full_name: Toups, Melissa A
id: 4E099E4E-F248-11E8-B48F-1D18A9856A87
last_name: Toups
orcid: 0000-0002-9752-7380
- first_name: Vincent K
full_name: Bett, Vincent K
id: 57854184-AAE0-11E9-8D04-98D6E5697425
last_name: Bett
- first_name: Andrea
full_name: Mrnjavac, Andrea
id: 353FAC84-AE61-11E9-8BFC-00D3E5697425
last_name: Mrnjavac
- first_name: Ariana
full_name: Macon, Ariana
id: 2A0848E2-F248-11E8-B48F-1D18A9856A87
last_name: Macon
- first_name: Christelle
full_name: Fraisse, Christelle
id: 32DF5794-F248-11E8-B48F-1D18A9856A87
last_name: Fraisse
orcid: 0000-0001-8441-5075
- first_name: Luca
full_name: Sax, Luca
id: 701c5602-97d8-11ed-96b5-b52773c70189
last_name: Sax
- first_name: Ann K
full_name: Huylmans, Ann K
id: 4C0A3874-F248-11E8-B48F-1D18A9856A87
last_name: Huylmans
orcid: 0000-0001-8871-4961
- first_name: Francisco
full_name: Hontoria, Francisco
last_name: Hontoria
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
citation:
ama: Elkrewi MN, Khauratovich U, Toups MA, et al. ZW sex-chromosome evolution and
contagious parthenogenesis in Artemia brine shrimp. Genetics. 2022;222(2).
doi:10.1093/genetics/iyac123
apa: Elkrewi, M. N., Khauratovich, U., Toups, M. A., Bett, V. K., Mrnjavac, A.,
Macon, A., … Vicoso, B. (2022). ZW sex-chromosome evolution and contagious parthenogenesis
in Artemia brine shrimp. Genetics. Oxford University Press. https://doi.org/10.1093/genetics/iyac123
chicago: Elkrewi, Marwan N, Uladzislava Khauratovich, Melissa A Toups, Vincent K
Bett, Andrea Mrnjavac, Ariana Macon, Christelle Fraisse, et al. “ZW Sex-Chromosome
Evolution and Contagious Parthenogenesis in Artemia Brine Shrimp.” Genetics.
Oxford University Press, 2022. https://doi.org/10.1093/genetics/iyac123.
ieee: M. N. Elkrewi et al., “ZW sex-chromosome evolution and contagious parthenogenesis
in Artemia brine shrimp,” Genetics, vol. 222, no. 2. Oxford University
Press, 2022.
ista: Elkrewi MN, Khauratovich U, Toups MA, Bett VK, Mrnjavac A, Macon A, Fraisse
C, Sax L, Huylmans AK, Hontoria F, Vicoso B. 2022. ZW sex-chromosome evolution
and contagious parthenogenesis in Artemia brine shrimp. Genetics. 222(2), iyac123.
mla: Elkrewi, Marwan N., et al. “ZW Sex-Chromosome Evolution and Contagious Parthenogenesis
in Artemia Brine Shrimp.” Genetics, vol. 222, no. 2, iyac123, Oxford University
Press, 2022, doi:10.1093/genetics/iyac123.
short: M.N. Elkrewi, U. Khauratovich, M.A. Toups, V.K. Bett, A. Mrnjavac, A. Macon,
C. Fraisse, L. Sax, A.K. Huylmans, F. Hontoria, B. Vicoso, Genetics 222 (2022).
corr_author: '1'
date_created: 2023-01-16T09:56:10Z
date_published: 2022-10-01T00:00:00Z
date_updated: 2026-06-15T22:31:14Z
day: '01'
ddc:
- '570'
department:
- _id: BeVi
doi: 10.1093/genetics/iyac123
ec_funded: 1
external_id:
isi:
- '000850270300001'
pmid:
- '35977389'
file:
- access_level: open_access
checksum: f79ff5383e882ea3f95f3da47a78029d
content_type: application/pdf
creator: dernst
date_created: 2023-01-30T08:59:58Z
date_updated: 2023-01-30T08:59:58Z
file_id: '12440'
file_name: 2022_Genetics_Elkrewi.pdf
file_size: 1347136
relation: main_file
success: 1
file_date_updated: 2023-01-30T08:59:58Z
has_accepted_license: '1'
intvolume: ' 222'
isi: 1
issue: '2'
keyword:
- Genetics
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 250BDE62-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715257'
name: Prevalence and Influence of Sexual Antagonism on Genome Evolution
- _id: 34ae1506-11ca-11ed-8bc3-c14f4c474396
grant_number: F8810
name: The highjacking of meiosis for asexual reproduction
publication: Genetics
publication_identifier:
issn:
- 1943-2631
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
record:
- id: '11653'
relation: research_data
status: public
- id: '19386'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine
shrimp
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 222
year: '2022'
...
---
_id: '10767'
abstract:
- lang: eng
text: The t-haplotype of mice is a classical model for autosomal transmission distortion.
A largely non-recombining variant of the proximal region of chromosome 17, it
is transmitted to more than 90% of the progeny of heterozygous males through the
disabling of sperm carrying a standard chromosome. While extensive genetic and
functional work has shed light on individual genes involved in drive, much less
is known about the evolution and function of the rest of its hundreds of genes.
Here, we characterize the sequence and expression of dozens of t-specific transcripts
and of their chromosome 17 homologues. Many genes showed reduced expression of
the t-allele, but an equal number of genes showed increased expression of their
t-copy, consistent with increased activity or a newly evolved function. Genes
on the t-haplotype had a significantly higher non-synonymous substitution rate
than their homologues on the standard chromosome, with several genes harbouring
dN/dS ratios above 1. Finally, the t-haplotype has acquired at least two genes
from other chromosomes, which show high and tissue-specific expression. These
results provide a first overview of the gene content of this selfish element,
and support a more dynamic evolutionary scenario than expected of a large genomic
region with almost no recombination.
acknowledgement: "This project has received funding from the European Research Council
under the European Union’s Horizon 2020 research and innovation program (grant agreement
no. 715257) and from the Swiss National Science Foundation (grant no. 310030_189145).\r\nWe
thank Jari Garbely of the Department of Evolutionary Biology and Environmental Studies,
University of Zurich, Zurich, Switzerland, for conducting the PCR verification.
Barbara\r\nKonig, Gabi Stichel and A.K.L. collected mouse tissue samples, from the
field study led by R.K.K. "
article_processing_charge: No
article_type: original
author:
- first_name: Réka K
full_name: Kelemen, Réka K
id: 48D3F8DE-F248-11E8-B48F-1D18A9856A87
last_name: Kelemen
orcid: 0000-0002-8489-9281
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Anna K.
full_name: Lindholm, Anna K.
last_name: Lindholm
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
citation:
ama: 'Kelemen RK, Elkrewi MN, Lindholm AK, Vicoso B. Novel patterns of expression
and recruitment of new genes on the t-haplotype, a mouse selfish chromosome. Proceedings
of the Royal Society B: Biological Sciences. 2022;289(1968):20211985. doi:10.1098/rspb.2021.1985'
apa: 'Kelemen, R. K., Elkrewi, M. N., Lindholm, A. K., & Vicoso, B. (2022).
Novel patterns of expression and recruitment of new genes on the t-haplotype,
a mouse selfish chromosome. Proceedings of the Royal Society B: Biological
Sciences. The Royal Society. https://doi.org/10.1098/rspb.2021.1985'
chicago: 'Kelemen, Réka K, Marwan N Elkrewi, Anna K. Lindholm, and Beatriz Vicoso.
“Novel Patterns of Expression and Recruitment of New Genes on the T-Haplotype,
a Mouse Selfish Chromosome.” Proceedings of the Royal Society B: Biological
Sciences. The Royal Society, 2022. https://doi.org/10.1098/rspb.2021.1985.'
ieee: 'R. K. Kelemen, M. N. Elkrewi, A. K. Lindholm, and B. Vicoso, “Novel patterns
of expression and recruitment of new genes on the t-haplotype, a mouse selfish
chromosome,” Proceedings of the Royal Society B: Biological Sciences, vol.
289, no. 1968. The Royal Society, p. 20211985, 2022.'
ista: 'Kelemen RK, Elkrewi MN, Lindholm AK, Vicoso B. 2022. Novel patterns of expression
and recruitment of new genes on the t-haplotype, a mouse selfish chromosome. Proceedings
of the Royal Society B: Biological Sciences. 289(1968), 20211985.'
mla: 'Kelemen, Réka K., et al. “Novel Patterns of Expression and Recruitment of
New Genes on the T-Haplotype, a Mouse Selfish Chromosome.” Proceedings of the
Royal Society B: Biological Sciences, vol. 289, no. 1968, The Royal Society,
2022, p. 20211985, doi:10.1098/rspb.2021.1985.'
short: 'R.K. Kelemen, M.N. Elkrewi, A.K. Lindholm, B. Vicoso, Proceedings of the
Royal Society B: Biological Sciences 289 (2022) 20211985.'
corr_author: '1'
date_created: 2022-02-20T23:01:31Z
date_published: 2022-02-09T00:00:00Z
date_updated: 2026-06-15T22:31:14Z
day: '09'
ddc:
- '570'
department:
- _id: BeVi
doi: 10.1098/rspb.2021.1985
ec_funded: 1
external_id:
isi:
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pmid:
- '35135349'
file:
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call_identifier: H2020
grant_number: '715257'
name: Prevalence and Influence of Sexual Antagonism on Genome Evolution
publication: 'Proceedings of the Royal Society B: Biological Sciences'
publication_identifier:
eissn:
- 1471-2954
publication_status: published
publisher: The Royal Society
quality_controlled: '1'
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relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Novel patterns of expression and recruitment of new genes on the t-haplotype,
a mouse selfish chromosome
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 289
year: '2022'
...
---
_id: '10167'
abstract:
- lang: eng
text: Schistosomes, the human parasites responsible for snail fever, are female-heterogametic.
Different parts of their ZW sex chromosomes have stopped recombining in distinct
lineages, creating “evolutionary strata” of various ages. Although the Z-chromosome
is well characterized at the genomic and molecular level, the W-chromosome has
remained largely unstudied from an evolutionary perspective, as only a few W-linked
genes have been detected outside of the model species Schistosoma mansoni. Here,
we characterize the gene content and evolution of the W-chromosomes of S. mansoni
and of the divergent species S. japonicum. We use a combined RNA/DNA k-mer based
pipeline to assemble around 100 candidate W-specific transcripts in each of the
species. About half of them map to known protein coding genes, the majority homologous
to S. mansoni Z-linked genes. We perform an extended analysis of the evolutionary
strata present in the two species (including characterizing a previously undetected
young stratum in S. japonicum) to infer patterns of sequence and expression evolution
of W-linked genes at different time points after recombination was lost. W-linked
genes show evidence of degeneration, including high rates of protein evolution
and reduced expression. Most are found in young lineage-specific strata, with
only a few high expression ancestral W-genes remaining, consistent with the progressive
erosion of nonrecombining regions. Among these, the splicing factor u2af2 stands
out as a promising candidate for primary sex determination, opening new avenues
for understanding the molecular basis of the reproductive biology of this group.
acknowledged_ssus:
- _id: ScienComp
acknowledgement: The authors thank IT support at IST Austria for providing an optimal
environment for bioinformatic analyses. This work was supported by an Austrian Science
Foundation FWF grant (Project P28842) to B.V.
article_processing_charge: No
article_type: original
author:
- first_name: Marwan N
full_name: Elkrewi, Marwan N
id: 0B46FACA-A8E1-11E9-9BD3-79D1E5697425
last_name: Elkrewi
orcid: 0000-0002-5328-7231
- first_name: Mikhail A.
full_name: Moldovan, Mikhail A.
id: c8bb7f32-3315-11ec-b58b-e5950e6c14a0
last_name: Moldovan
orcid: 0000-0002-8876-6494
- first_name: Marion A L
full_name: Picard, Marion A L
id: 2C921A7A-F248-11E8-B48F-1D18A9856A87
last_name: Picard
orcid: 0000-0002-8101-2518
- first_name: Beatriz
full_name: Vicoso, Beatriz
id: 49E1C5C6-F248-11E8-B48F-1D18A9856A87
last_name: Vicoso
orcid: 0000-0002-4579-8306
citation:
ama: Elkrewi MN, Moldovan MA, Picard MAL, Vicoso B. Schistosome W-linked genes inform
temporal dynamics of sex chromosome evolution and suggest candidate for sex determination.
Molecular Biology and Evolution. 2021;138(12):5345-5358. doi:10.1093/molbev/msab178
apa: Elkrewi, M. N., Moldovan, M. A., Picard, M. A. L., & Vicoso, B. (2021).
Schistosome W-linked genes inform temporal dynamics of sex chromosome evolution
and suggest candidate for sex determination. Molecular Biology and Evolution.
Oxford University Press . https://doi.org/10.1093/molbev/msab178
chicago: Elkrewi, Marwan N, Mikhail A. Moldovan, Marion A L Picard, and Beatriz
Vicoso. “Schistosome W-Linked Genes Inform Temporal Dynamics of Sex Chromosome
Evolution and Suggest Candidate for Sex Determination.” Molecular Biology and
Evolution. Oxford University Press , 2021. https://doi.org/10.1093/molbev/msab178.
ieee: M. N. Elkrewi, M. A. Moldovan, M. A. L. Picard, and B. Vicoso, “Schistosome
W-linked genes inform temporal dynamics of sex chromosome evolution and suggest
candidate for sex determination,” Molecular Biology and Evolution, vol.
138, no. 12. Oxford University Press , pp. 5345–58, 2021.
ista: Elkrewi MN, Moldovan MA, Picard MAL, Vicoso B. 2021. Schistosome W-linked
genes inform temporal dynamics of sex chromosome evolution and suggest candidate
for sex determination. Molecular Biology and Evolution. 138(12), 5345–58.
mla: Elkrewi, Marwan N., et al. “Schistosome W-Linked Genes Inform Temporal Dynamics
of Sex Chromosome Evolution and Suggest Candidate for Sex Determination.” Molecular
Biology and Evolution, vol. 138, no. 12, Oxford University Press , 2021, pp.
5345–58, doi:10.1093/molbev/msab178.
short: M.N. Elkrewi, M.A. Moldovan, M.A.L. Picard, B. Vicoso, Molecular Biology
and Evolution 138 (2021) 5345–58.
corr_author: '1'
date_created: 2021-10-21T07:49:12Z
date_published: 2021-06-19T00:00:00Z
date_updated: 2026-06-15T22:31:14Z
day: '19'
ddc:
- '610'
department:
- _id: BeVi
doi: 10.1093/molbev/msab178
external_id:
isi:
- '000741368600009'
pmid:
- '34146097'
file:
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checksum: 1b096702fb356d9c0eb88e1b3fcff5f8
content_type: application/pdf
creator: dernst
date_created: 2022-05-06T09:47:18Z
date_updated: 2022-05-06T09:47:18Z
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file_size: 1008594
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success: 1
file_date_updated: 2022-05-06T09:47:18Z
has_accepted_license: '1'
intvolume: ' 138'
isi: 1
issue: '12'
keyword:
- sex chromosomes
- evolutionary strata
- W-linked gene
- sex determining gene
- schistosome parasites
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 5345-58
pmid: 1
project:
- _id: 250ED89C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P28842-B22
name: Sex chromosome evolution under male- and female- heterogamety
publication: Molecular Biology and Evolution
publication_identifier:
eissn:
- 1537-1719
issn:
- 0737-4038
publication_status: published
publisher: 'Oxford University Press '
quality_controlled: '1'
related_material:
record:
- id: '19386'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Schistosome W-linked genes inform temporal dynamics of sex chromosome evolution
and suggest candidate for sex determination
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 138
year: '2021'
...