---
OA_place: publisher
OA_type: gold
_id: '19010'
abstract:
- lang: eng
  text: Causal representation learning aims at recovering latent causal variables
    from high-dimensional observations to solve causal downstream tasks, such as predicting
    the effect of new interventions or more robust classification. A plethora of methods
    have been developed, each tackling carefully crafted problem settings that lead
    to different types of identifiability. The folklore is that these different settings
    are important, as they are often linked to different rungs of Pearl's causal hierarchy,
    although not all neatly fit. Our main contribution is to show that many existing
    causal representation learning approaches methodologically align the representation
    to known data symmetries. Identification of the variables is guided by equivalence
    classes across different "data pockets" that are not necessarily causal. This
    result suggests important implications, allowing us to unify many existing approaches
    in a single method that can mix and match different assumptions, including non-causal
    ones, based on the invariances relevant to our application. It also significantly
    benefits applicability, which we demonstrate by improving treatment effect estimation
    on real-world high-dimensional ecological data. Overall, this paper clarifies
    the role of causality assumptions in the discovery of causal variables and shifts
    the focus to preserving data symmetries.
acknowledgement: "We thank Jiaqi Zhang, Francesco Montagna, David Lopez-Paz, Kartik
  Ahuja, Thomas Kipf, Sara\r\nMagliacane, Julius von Kügelgen, Kun Zhang, and Bernhard
  Schölkopf for extremely helpful discussion. Riccardo Cadei was supported by a Google
  Research Scholar Award to Francesco Locatello. We acknowledge the Third Bellairs
  Workshop on Causal Representation Learning held at the Bellairs Research Institute,
  February 9/16, 2024, and a debate on the difference between interventions and counterfactuals
  in disentanglement and CRL that took place during Dhanya Sridhar’s lecture, which
  motivated us to significantly broaden the scope of the paper. We thank Dhanya and
  all participants of the workshop."
article_processing_charge: No
arxiv: 1
author:
- first_name: Dingling
  full_name: Yao, Dingling
  id: d3e02e50-48a8-11ee-8f62-c108061797fa
  last_name: Yao
- first_name: Dario
  full_name: Rancati, Dario
  id: feb58f2e-72ef-11ef-b75a-8f0894539cd0
  last_name: Rancati
- first_name: Riccardo
  full_name: Cadei, Riccardo
  id: 0fa8b76f-72f0-11ef-b75a-a5da96e5ad6b
  last_name: Cadei
- first_name: Marco
  full_name: Fumero, Marco
  id: 1c1593eb-393f-11ef-bb8e-ab4f1e979650
  last_name: Fumero
- first_name: Francesco
  full_name: Locatello, Francesco
  id: 26cfd52f-2483-11ee-8040-88983bcc06d4
  last_name: Locatello
  orcid: 0000-0002-4850-0683
citation:
  ama: 'Yao D, Rancati D, Cadei R, Fumero M, Locatello F. Unifying causal representation
    learning with the invariance principle. In: <i>13th International Conference on
    Learning Representations</i>. ICLR; 2025.'
  apa: 'Yao, D., Rancati, D., Cadei, R., Fumero, M., &#38; Locatello, F. (2025). Unifying
    causal representation learning with the invariance principle. In <i>13th International
    Conference on Learning Representations</i>. Singapore: ICLR.'
  chicago: Yao, Dingling, Dario Rancati, Riccardo Cadei, Marco Fumero, and Francesco
    Locatello. “Unifying Causal Representation Learning with the Invariance Principle.”
    In <i>13th International Conference on Learning Representations</i>. ICLR, 2025.
  ieee: D. Yao, D. Rancati, R. Cadei, M. Fumero, and F. Locatello, “Unifying causal
    representation learning with the invariance principle,” in <i>13th International
    Conference on Learning Representations</i>, Singapore, 2025.
  ista: 'Yao D, Rancati D, Cadei R, Fumero M, Locatello F. 2025. Unifying causal representation
    learning with the invariance principle. 13th International Conference on Learning
    Representations. ICLR: International Conference on Learning Representations.'
  mla: Yao, Dingling, et al. “Unifying Causal Representation Learning with the Invariance
    Principle.” <i>13th International Conference on Learning Representations</i>,
    ICLR, 2025.
  short: D. Yao, D. Rancati, R. Cadei, M. Fumero, F. Locatello, in:, 13th International
    Conference on Learning Representations, ICLR, 2025.
conference:
  end_date: 2025-04-28
  location: Singapore
  name: 'ICLR: International Conference on Learning Representations'
  start_date: 2025-04-24
corr_author: '1'
date_created: 2025-02-05T09:23:25Z
date_published: 2025-01-22T00:00:00Z
date_updated: 2026-02-09T05:52:14Z
day: '22'
ddc:
- '000'
department:
- _id: FrLo
external_id:
  arxiv:
  - '2409.02772'
file:
- access_level: open_access
  checksum: c4b5a4a644228c6d1b0283e1368bce9e
  content_type: application/pdf
  creator: flocatel
  date_created: 2026-01-27T12:43:25Z
  date_updated: 2026-01-27T12:43:25Z
  file_id: '21048'
  file_name: 4356_Unifying_Causal_Represent (1).pdf
  file_size: 877014
  relation: main_file
  success: 1
file_date_updated: 2026-01-27T12:43:25Z
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
publication: 13th International Conference on Learning Representations
publication_status: published
publisher: ICLR
quality_controlled: '1'
scopus_import: '1'
status: public
title: Unifying causal representation learning with the invariance principle
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2025'
...
---
OA_place: repository
OA_type: green
_id: '21068'
abstract:
- lang: eng
  text: "Causal reasoning and discovery, two fundamental tasks of causal analysis,\r\noften
    face challenges in applications due to the complexity, noisiness, and highdimensionality
    of real-world data. Despite recent progress in identifying latent\r\ncausal structures
    using causal representation learning (CRL), what makes learned\r\nrepresentations
    useful for causal downstream tasks and how to evaluate them are\r\nstill not well
    understood. In this paper, we reinterpret CRL using a measurement\r\nmodel framework,
    where the learned representations are viewed as proxy measurements of the latent
    causal variables. Our approach clarifies the conditions under\r\nwhich learned
    representations support downstream causal reasoning and provides\r\na principled
    basis for quantitatively assessing the quality of representations using\r\na new
    Test-based Measurement EXclusivity (T-MEX) score. We validate T-MEX\r\nacross
    diverse causal inference scenarios, including numerical simulations and\r\nreal-world
    ecological video analysis, demonstrating that the proposed framework\r\nand corresponding
    score effectively assess the identification of learned representations and their
    usefulness for causal downstream tasks. Reproducible code can\r\nbe found at https://github.com/shimenghuang/a-measurement-perspective-of-crl."
acknowledgement: "This research was funded in whole or in part by the Austrian Science
  Fund (FWF) 10.55776/COE12. For open access purposes, the author has applied a CC
  BY public copyright license to any accepted manuscript version arising from this
  submission.\r\n"
alternative_title:
- Advances in Neural Information Processing Systems
article_processing_charge: No
arxiv: 1
author:
- first_name: Dingling
  full_name: Yao, Dingling
  id: d3e02e50-48a8-11ee-8f62-c108061797fa
  last_name: Yao
- first_name: Shimeng
  full_name: Huang, Shimeng
  id: 989c2a06-fb4e-11ef-a992-ab766442255b
  last_name: Huang
  orcid: 0000-0001-6919-821X
- first_name: Riccardo
  full_name: Cadei, Riccardo
  id: 0fa8b76f-72f0-11ef-b75a-a5da96e5ad6b
  last_name: Cadei
- first_name: Kun
  full_name: Zhang, Kun
  last_name: Zhang
- first_name: Francesco
  full_name: Locatello, Francesco
  id: 26cfd52f-2483-11ee-8040-88983bcc06d4
  last_name: Locatello
  orcid: 0000-0002-4850-0683
citation:
  ama: 'Yao D, Huang S, Cadei R, Zhang K, Locatello F. The third pillar of causal
    analysis? A measurement perspective on causal representations. In: <i>39th Annual
    Conference on Neural Information Processing Systems</i>. Vol 38. Neural Information
    Processing Systems Foundation; 2025.'
  apa: 'Yao, D., Huang, S., Cadei, R., Zhang, K., &#38; Locatello, F. (2025). The
    third pillar of causal analysis? A measurement perspective on causal representations.
    In <i>39th Annual Conference on Neural Information Processing Systems</i> (Vol.
    38). San Diego, CA, United States: Neural Information Processing Systems Foundation.'
  chicago: Yao, Dingling, Shimeng Huang, Riccardo Cadei, Kun Zhang, and Francesco
    Locatello. “The Third Pillar of Causal Analysis? A Measurement Perspective on
    Causal Representations.” In <i>39th Annual Conference on Neural Information Processing
    Systems</i>, Vol. 38. Neural Information Processing Systems Foundation, 2025.
  ieee: D. Yao, S. Huang, R. Cadei, K. Zhang, and F. Locatello, “The third pillar
    of causal analysis? A measurement perspective on causal representations,” in <i>39th
    Annual Conference on Neural Information Processing Systems</i>, San Diego, CA,
    United States, 2025, vol. 38.
  ista: 'Yao D, Huang S, Cadei R, Zhang K, Locatello F. 2025. The third pillar of
    causal analysis? A measurement perspective on causal representations. 39th Annual
    Conference on Neural Information Processing Systems. NeurIPS: Neural Information
    Processing Systems, Advances in Neural Information Processing Systems, vol. 38.'
  mla: Yao, Dingling, et al. “The Third Pillar of Causal Analysis? A Measurement Perspective
    on Causal Representations.” <i>39th Annual Conference on Neural Information Processing
    Systems</i>, vol. 38, Neural Information Processing Systems Foundation, 2025.
  short: D. Yao, S. Huang, R. Cadei, K. Zhang, F. Locatello, in:, 39th Annual Conference
    on Neural Information Processing Systems, Neural Information Processing Systems
    Foundation, 2025.
conference:
  end_date: 2025-12-07
  location: San Diego, CA, United States
  name: 'NeurIPS: Neural Information Processing Systems'
  start_date: 2025-12-02
corr_author: '1'
date_created: 2026-01-29T14:24:56Z
date_published: 2025-12-15T00:00:00Z
date_updated: 2026-02-10T12:08:52Z
day: '15'
ddc:
- '000'
department:
- _id: FrLo
external_id:
  arxiv:
  - '2505.17708'
has_accepted_license: '1'
intvolume: '        38'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.48550/arXiv.2505.17708
month: '12'
oa: 1
oa_version: Preprint
publication: 39th Annual Conference on Neural Information Processing Systems
publication_identifier:
  issn:
  - 1049-5258
publication_status: epub_ahead
publisher: Neural Information Processing Systems Foundation
quality_controlled: '1'
related_material:
  link:
  - relation: software
    url: https://github.com/shimenghuang/a-measurement-perspective-of-crl
status: public
title: The third pillar of causal analysis? A measurement perspective on causal representations
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
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  short: CC BY (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 38
year: '2025'
...
---
OA_place: publisher
OA_type: gold
_id: '21076'
abstract:
- lang: eng
  text: In many scientific experiments, the data annotating cost constraints the pace
    for testing novel hypotheses. Yet, modern machine learning pipelines offer a promising
    solution—provided their predictions yield correct conclusions. We focus on Prediction-Powered
    Causal Inferences (PPCI), i.e., estimating the treatment effect in an unlabeled
    target experiment, relying on training data with the same outcome annotated but
    potentially different treatment or effect modifiers. We first show that conditional
    calibration guarantees valid PPCI at population level. Then, we introduce a sufficient
    representation constraint transferring validity across experiments, which we propose
    to enforce in practice in Deconfounded Empirical Risk Minimization, our new model-agnostic
    training objective. We validate our method on synthetic and real-world scientific
    data, solving impossible problem instances for Empirical Risk Minimization even
    with standard invariance constraints. In particular, for the first time, we achieve
    valid causal inference on a scientific experiment with complex recording and no
    human annotations, fine-tuning a foundational model on our similar annotated experiment.
acknowledgement: We thank the Causal Learning and Artificial Intelligence group at
  ISTA for the continuous feedback on the project and valuable discussions. We thank
  the Social Immunity group at ISTA, particularly Jinook Oh, for the annotation program
  and Michaela Hoenigsberger for supporting our ecological experiment. Riccardo Cadei
  is supported by a Google Research Scholar Award and a Google Initiated Gift to Francesco
  Locatello. This research was funded in part by the Austrian Science Fund (FWF) 10.55776/COE12).
  It was further partially supported by the ISTA Interdisciplinary Project Committee
  for the collaborative project “ALED” between Francesco Locatello and Sylvia Cremer.
  For open access purposes, the author has applied a CC BY public copyright license
  to any author accepted manuscript version arising from this submission.
alternative_title:
- Advances in Neural Information Processing Systems
article_processing_charge: No
author:
- first_name: Riccardo
  full_name: Cadei, Riccardo
  id: 0fa8b76f-72f0-11ef-b75a-a5da96e5ad6b
  last_name: Cadei
- first_name: Ilker
  full_name: Demirel, Ilker
  last_name: Demirel
- first_name: Piersilvio
  full_name: De Bartolomeis, Piersilvio
  last_name: De Bartolomeis
- first_name: Lukas
  full_name: Lindorfer, Lukas
  id: 85f0e6d3-06b3-11ec-8982-8c5049fa4455
  last_name: Lindorfer
- first_name: Sylvia
  full_name: Cremer, Sylvia
  id: 2F64EC8C-F248-11E8-B48F-1D18A9856A87
  last_name: Cremer
  orcid: 0000-0002-2193-3868
- first_name: Cordelia
  full_name: Schmid, Cordelia
  last_name: Schmid
- first_name: Francesco
  full_name: Locatello, Francesco
  id: 26cfd52f-2483-11ee-8040-88983bcc06d4
  last_name: Locatello
  orcid: 0000-0002-4850-0683
citation:
  ama: 'Cadei R, Demirel I, De Bartolomeis P, et al. Prediction-powered causal inferences.
    In: <i>39th Annual Conference on Neural Information Processing Systems</i>. Vol
    38. Neural Information Processing Systems Foundation; 2025.'
  apa: 'Cadei, R., Demirel, I., De Bartolomeis, P., Lindorfer, L., Cremer, S., Schmid,
    C., &#38; Locatello, F. (2025). Prediction-powered causal inferences. In <i>39th
    Annual Conference on Neural Information Processing Systems</i> (Vol. 38). San
    Diego, CA, United States: Neural Information Processing Systems Foundation.'
  chicago: Cadei, Riccardo, Ilker Demirel, Piersilvio De Bartolomeis, Lukas Lindorfer,
    Sylvia Cremer, Cordelia Schmid, and Francesco Locatello. “Prediction-Powered Causal
    Inferences.” In <i>39th Annual Conference on Neural Information Processing Systems</i>,
    Vol. 38. Neural Information Processing Systems Foundation, 2025.
  ieee: R. Cadei <i>et al.</i>, “Prediction-powered causal inferences,” in <i>39th
    Annual Conference on Neural Information Processing Systems</i>, San Diego, CA,
    United States, 2025, vol. 38.
  ista: 'Cadei R, Demirel I, De Bartolomeis P, Lindorfer L, Cremer S, Schmid C, Locatello
    F. 2025. Prediction-powered causal inferences. 39th Annual Conference on Neural
    Information Processing Systems. NeurIPS: Neural Information Processing Systems,
    Advances in Neural Information Processing Systems, vol. 38.'
  mla: Cadei, Riccardo, et al. “Prediction-Powered Causal Inferences.” <i>39th Annual
    Conference on Neural Information Processing Systems</i>, vol. 38, Neural Information
    Processing Systems Foundation, 2025.
  short: R. Cadei, I. Demirel, P. De Bartolomeis, L. Lindorfer, S. Cremer, C. Schmid,
    F. Locatello, in:, 39th Annual Conference on Neural Information Processing Systems,
    Neural Information Processing Systems Foundation, 2025.
conference:
  end_date: 2025-12-07
  location: San Diego, CA, United States
  name: 'NeurIPS: Neural Information Processing Systems'
  start_date: 2025-12-02
date_created: 2026-01-29T14:35:11Z
date_published: 2025-12-15T00:00:00Z
date_updated: 2026-02-16T11:39:33Z
day: '15'
ddc:
- '000'
department:
- _id: FrLo
- _id: SyCr
file:
- access_level: open_access
  checksum: 92467fa566cd36671a6a3b9e71ae0f71
  content_type: application/pdf
  creator: flocatel
  date_created: 2026-01-29T14:35:02Z
  date_updated: 2026-01-29T14:35:02Z
  file_id: '21077'
  file_name: 17546_Prediction_Powered_Causa.pdf
  file_size: 8489023
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file_date_updated: 2026-01-29T14:35:02Z
has_accepted_license: '1'
intvolume: '        38'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
publication: 39th Annual Conference on Neural Information Processing Systems
publication_identifier:
  issn:
  - 1049-5258
publication_status: epub_ahead
publisher: Neural Information Processing Systems Foundation
quality_controlled: '1'
status: public
title: Prediction-powered causal inferences
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
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  short: CC BY (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 38
year: '2025'
...
---
OA_place: publisher
OA_type: gold
_id: '18847'
abstract:
- lang: eng
  text: "Machine Learning and AI have the potential to transform data-driven\r\nscientific
    discovery, enabling accurate predictions for several scientific\r\nphenomena.
    As many scientific questions are inherently causal, this paper looks\r\nat the
    causal inference task of treatment effect estimation, where the outcome\r\nof
    interest is recorded in high-dimensional observations in a Randomized\r\nControlled
    Trial (RCT). Despite being the simplest possible causal setting and\r\na perfect
    fit for deep learning, we theoretically find that many common choices\r\nin the
    literature may lead to biased estimates. To test the practical impact of\r\nthese
    considerations, we recorded ISTAnt, the first real-world benchmark for\r\ncausal
    inference downstream tasks on high-dimensional observations as an RCT\r\nstudying
    how garden ants (Lasius neglectus) respond to microparticles applied\r\nonto their
    colony members by hygienic grooming. Comparing 6 480 models\r\nfine-tuned from
    state-of-the-art visual backbones, we find that the sampling\r\nand modeling choices
    significantly affect the accuracy of the causal estimate,\r\nand that classification
    accuracy is not a proxy thereof. We further validated\r\nthe analysis, repeating
    it on a synthetically generated visual data set\r\ncontrolling the causal model.
    Our results suggest that future benchmarks should\r\ncarefully consider real downstream
    scientific questions, especially causal\r\nones. Further, we highlight guidelines
    for representation learning methods to\r\nhelp answer causal questions in the
    sciences."
acknowledgement: We thank Piersilvio De Bartolomeis, and the full Causal Learning
  and Artificial Intelligence (CLAI) group at ISTA for the extremely helpful discussions.
  Riccardo Cadei was supported by a Google Research Scholar Award and a Google Initiated
  Gift to Francesco Locatello. We thank the Social Immunity team at ISTA particularly
  Michaela Hönigsberger and Wilfrid Jean Louis, for supporting the ecological experiment
  and Farnaz Beikzadeh Abbasi, Luisa Fiebig and Martin Estermann for annotating ant
  behavior in ISTAnt.
article_processing_charge: No
arxiv: 1
author:
- first_name: Riccardo
  full_name: Cadei, Riccardo
  id: 0fa8b76f-72f0-11ef-b75a-a5da96e5ad6b
  last_name: Cadei
- first_name: Lukas
  full_name: Lindorfer, Lukas
  id: 85f0e6d3-06b3-11ec-8982-8c5049fa4455
  last_name: Lindorfer
- first_name: Sylvia
  full_name: Cremer, Sylvia
  id: 2F64EC8C-F248-11E8-B48F-1D18A9856A87
  last_name: Cremer
  orcid: 0000-0002-2193-3868
- first_name: Cordelia
  full_name: Schmid, Cordelia
  last_name: Schmid
- first_name: Francesco
  full_name: Locatello, Francesco
  id: 26cfd52f-2483-11ee-8040-88983bcc06d4
  last_name: Locatello
  orcid: 0000-0002-4850-0683
citation:
  ama: 'Cadei R, Lindorfer L, Cremer S, Schmid C, Locatello F. Smoke and mirrors in
    causal downstream tasks. In: <i>ICML 2024 Workshop AI4Science</i>. Vol 38. Curran
    Associates; 2024.'
  apa: Cadei, R., Lindorfer, L., Cremer, S., Schmid, C., &#38; Locatello, F. (2024).
    Smoke and mirrors in causal downstream tasks. In <i>ICML 2024 Workshop AI4Science</i>
    (Vol. 38). Curran Associates.
  chicago: Cadei, Riccardo, Lukas Lindorfer, Sylvia Cremer, Cordelia Schmid, and Francesco
    Locatello. “Smoke and Mirrors in Causal Downstream Tasks.” In <i>ICML 2024 Workshop
    AI4Science</i>, Vol. 38. Curran Associates, 2024.
  ieee: R. Cadei, L. Lindorfer, S. Cremer, C. Schmid, and F. Locatello, “Smoke and
    mirrors in causal downstream tasks,” in <i>ICML 2024 Workshop AI4Science</i>,
    2024, vol. 38.
  ista: 'Cadei R, Lindorfer L, Cremer S, Schmid C, Locatello F. 2024. Smoke and mirrors
    in causal downstream tasks. ICML 2024 Workshop AI4Science. ICML: International
    Conference on Machine Learning vol. 38.'
  mla: Cadei, Riccardo, et al. “Smoke and Mirrors in Causal Downstream Tasks.” <i>ICML
    2024 Workshop AI4Science</i>, vol. 38, Curran Associates, 2024.
  short: R. Cadei, L. Lindorfer, S. Cremer, C. Schmid, F. Locatello, in:, ICML 2024
    Workshop AI4Science, Curran Associates, 2024.
conference:
  end_date: 2024-07-26
  name: 'ICML: International Conference on Machine Learning'
  start_date: 2024-07-26
corr_author: '1'
date_created: 2025-01-14T07:27:26Z
date_published: 2024-09-25T00:00:00Z
date_updated: 2025-07-10T11:51:50Z
day: '25'
ddc:
- '000'
- '570'
department:
- _id: SyCr
- _id: FrLo
- _id: GradSch
external_id:
  arxiv:
  - '2405.17151'
file:
- access_level: open_access
  checksum: beedf05388bbdb7ddda81ec3d5ec7026
  content_type: application/pdf
  creator: dernst
  date_created: 2025-01-27T11:42:24Z
  date_updated: 2025-01-27T11:42:24Z
  file_id: '18896'
  file_name: 2024_ICML_Cadei.pdf
  file_size: 4453014
  relation: main_file
  success: 1
file_date_updated: 2025-01-27T11:42:24Z
has_accepted_license: '1'
intvolume: '        38'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
publication: ICML 2024 Workshop AI4Science
publication_status: published
publisher: Curran Associates
quality_controlled: '1'
related_material:
  link:
  - relation: software
    url: https://github.com/CausalLearningAI/ISTAnt
  record:
  - id: '18895'
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scopus_import: '1'
status: public
title: Smoke and mirrors in causal downstream tasks
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  short: CC BY (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 38
year: '2024'
...
---
OA_place: repository
OA_type: gold
_id: '18895'
abstract:
- lang: eng
  text: 'ISTAnt is a new ecological dataset for social immunity and represents the
    first real-world benchmark for causal inference downstream tasks on high-dimensional
    observations. It analyzes grooming behavior in the ant Lasius neglectus in groups
    of three worker ants. The workers for the experiment were obtained from their
    laboratory stock colony, which had been collected from the field in 2022 in the
    Botanical Garden Jena, Germany. Ant collection and all experimental work were
    performed in compliance with international, national and institutional regulations
    and ethical guidelines. For the experiment, the body surface of one of the three
    ants was treated with a suspension of either of two microparticle types (diameter
    ~5 µm) to induce grooming by the two nestmates, which were individually color-coded
    by application of a dot of blue or orange paint, respectively. The three ants
    were housed in small plastic containers (diameter 28mm, height 30mm) with moistened,
    plastered ground and the interior walls covered with PTFE (polytetrafluoroethane)
    to hamper climbing by the ants. Filming occurred in a temperature- and humidity-controlled
    room at 23°C within a custom-made filming box with controlled lighting and ventilation
    conditions. We set up nine ant groups at a time (always containing both treatments)
    and placed them randomly on positions 1-9 marked on the floor in a 3x3 grid, about
    3mm from each other. The experiment was performed on two consecutive days. Videos
    were acquired using a USB camera (FLIR blackfly S BFS-U3-120S4C, Teledyne FLIR)
    with a high-performance lens (HP Series 25mm Focal Length, Edmund optics 86-572)
    in OBS studio 29.0.0 \citep{bailey2017obs} at a framerate of 30 FPS and a resolution
    of 2500x2500 pixels. From each original video (105x105 mm), we generated nine
    individual videos .mkv (each ~32x32 mm, 770x770 pixels) by determining exact coordinates
    per container from one frame in GIMP 2.10.36 and cropping of the videos with FFmpeg
    6.1.1. Annotation was performed over two consecutive days by three observers who
    had not been involved in the experimental setup or recording and were unaware
    of the treatment assignments to ensure bias-free behavioral annotation. They annotated
    the behavior of the ants during video observations, using custom-made software
    that saves the start and end frames of behaviors marked in a .csv file (see ''annotations''
    folder). In one of the videos, one of the nestmates'' legs got inadvertently stuck
    to its body surface during the color-coding, interfering with its behavior, so
    the video was discarded. This left 44 videos from 5 independent setups (n=24 of
    treatment 1 and n=20 of treatment 2) of 10 minutes each for a total of 792 000
    annotated frames (see ''video'' folder). For each video, we provide the following
    information: the number of the set to which it belongs (1-5); the number of the
    position within the set reflecting the position of the ant group under the camera
    (1-9), for which we also provide ‘coordinates’ in the 3x3 grid (taking values
    -1/0/1 for both X and Y axis); treatment (1 or 2); the hour of the day when the
    recording was started (in 24h CEST); experimental day (A or B); the top left coordinate
    of the cropping square from the original video (CropX/CropY); the person annotating
    the video (given as A, B, C); the date of annotation (1: first day, 2: second
    day) and in which order the videos were annotated by each person, both reflecting
    a possible training effect of the person (see ''experiments_settings.csv'' file).'
article_processing_charge: No
author:
- first_name: Riccardo
  full_name: Cadei, Riccardo
  id: 0fa8b76f-72f0-11ef-b75a-a5da96e5ad6b
  last_name: Cadei
- first_name: Francesco
  full_name: Locatello, Francesco
  id: 26cfd52f-2483-11ee-8040-88983bcc06d4
  last_name: Locatello
  orcid: 0000-0002-4850-0683
- first_name: Sylvia M
  full_name: Cremer, Sylvia M
  id: 2F64EC8C-F248-11E8-B48F-1D18A9856A87
  last_name: Cremer
  orcid: 0000-0002-2193-3868
- first_name: Lukas
  full_name: Lindorfer, Lukas
  id: 85f0e6d3-06b3-11ec-8982-8c5049fa4455
  last_name: Lindorfer
- first_name: Cordelia
  full_name: Schmid, Cordelia
  last_name: Schmid
citation:
  ama: Cadei R, Locatello F, Cremer S, Lindorfer L, Schmid C. ISTAnt. 2024. doi:<a
    href="https://doi.org/10.6084/M9.FIGSHARE.26484934.V2">10.6084/M9.FIGSHARE.26484934.V2</a>
  apa: Cadei, R., Locatello, F., Cremer, S., Lindorfer, L., &#38; Schmid, C. (2024).
    ISTAnt. Institute of Science and Technology Austria. <a href="https://doi.org/10.6084/M9.FIGSHARE.26484934.V2">https://doi.org/10.6084/M9.FIGSHARE.26484934.V2</a>
  chicago: Cadei, Riccardo, Francesco Locatello, Sylvia Cremer, Lukas Lindorfer, and
    Cordelia Schmid. “ISTAnt.” Institute of Science and Technology Austria, 2024.
    <a href="https://doi.org/10.6084/M9.FIGSHARE.26484934.V2">https://doi.org/10.6084/M9.FIGSHARE.26484934.V2</a>.
  ieee: R. Cadei, F. Locatello, S. Cremer, L. Lindorfer, and C. Schmid, “ISTAnt.”
    Institute of Science and Technology Austria, 2024.
  ista: Cadei R, Locatello F, Cremer S, Lindorfer L, Schmid C. 2024. ISTAnt, Institute
    of Science and Technology Austria, <a href="https://doi.org/10.6084/M9.FIGSHARE.26484934.V2">10.6084/M9.FIGSHARE.26484934.V2</a>.
  mla: Cadei, Riccardo, et al. <i>ISTAnt</i>. Institute of Science and Technology
    Austria, 2024, doi:<a href="https://doi.org/10.6084/M9.FIGSHARE.26484934.V2">10.6084/M9.FIGSHARE.26484934.V2</a>.
  short: R. Cadei, F. Locatello, S. Cremer, L. Lindorfer, C. Schmid, (2024).
corr_author: '1'
date_created: 2025-01-27T11:45:43Z
date_published: 2024-10-23T00:00:00Z
date_updated: 2025-01-27T11:58:38Z
day: '23'
ddc:
- '570'
department:
- _id: SyCr
- _id: FrLo
- _id: GradSch
doi: 10.6084/M9.FIGSHARE.26484934.V2
main_file_link:
- open_access: '1'
  url: https://10.6084/M9.FIGSHARE.26484934.V2
month: '10'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '18847'
    relation: used_in_publication
    status: public
status: public
title: ISTAnt
type: research_data_reference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
