---
OA_place: repository
OA_type: green
_id: '21291'
abstract:
- lang: eng
  text: The complexity and specificity of movement in vertebrates is driven by a rich
    diversity of spinal motor and interneuron cell types. During development, eleven
    spinal cord progenitor domains generate an equivalent number of cardinal neuron
    types. How progenitor domains, individual progenitors, and post-mitotic diversity
    relate is still unknown. We performed high-resolution, single-progenitor cell
    lineage tracing in the embryonic mouse spinal cord using mosaic analysis with
    double markers (MADM). Our quantitative study of lineage progression revealed
    that spinal cord progenitors undergo highly variable numbers of proliferative,
    neurogenic, and gliogenic cell divisions. The nascent clonally-related neurons
    migrate radially over large distances, span the dorsoventral axis, and even cross
    the midline, demonstrating striking bilaterality. Molecular and morphometric analysis
    indicate high levels of progenitor multipotency, with an individual progenitor
    capable of producing several molecularly and morphologically distinct neuron types,
    as well as astrocytes. These findings redefine spinal cord development as a process
    in which lineage variability — rather than rigid progenitor identity — drives
    the generation of cellular diversity.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: "We would like to thank Elizabeth Marin, Anna Kicheva, Igor Adameyko,
  and James Briscoe as\r\nwell as members of the Sweeney and Hippemeyer labs and SFB
  consortium for comments on\r\nthe manuscript. We are also grateful for the technical
  support of the Preclinical and Imaging and\r\nOptics Facilities support teams (ISTA).
  In addition, we thank our funding sources for providing\r\nthe resources to do these
  experiments: Horizon Europe ERC Starting Grant Number 101041551\r\n(M.S.; L.B.S.);
  Special Research Program (SFB) of the Austrian Science Fund (FWF)\r\nNeuroStem Modulation
  Project numbers F7814-B (S.A.G.; M.S.; G.S.; and L.B.S.) and F7805\r\n(G.C. and
  S.H.). S.A.G is supported by a Boehringer Ingelheim Fonds PhD Fellowship, F.D.S.N.\r\nby
  an Institute of Science and Technology Austria (ISTA) GROW fellowship, and G.C.
  by an\r\nISTA Plus postdoctoral fellowship from the European Commission. S.H./L.B.S.
  and G.C. were\r\nadditionally supported by institutional funds from the ISTA and
  the University of Exeter,\r\nrespectively. "
article_processing_charge: No
author:
- first_name: Sophie A
  full_name: Gobeil, Sophie A
  id: 2f3e9efb-eb24-11ec-86b2-88efb11d59fa
  last_name: Gobeil
- first_name: Francisco
  full_name: Da Silveira Neto, Francisco
  id: 8cfb7412-10a7-11f1-add1-82b44e6418f2
  last_name: Da Silveira Neto
- first_name: Giulia
  full_name: Silvestrelli, Giulia
  id: 12632ae8-799e-11ef-94a2-e5a3b5ef49e9
  last_name: Silvestrelli
- first_name: Matthijs Geert
  full_name: Smits, Matthijs Geert
  id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0
  last_name: Smits
- first_name: Carmen
  full_name: Streicher, Carmen
  id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
  last_name: Streicher
- first_name: Giselle T
  full_name: Cheung, Giselle T
  id: 471195F6-F248-11E8-B48F-1D18A9856A87
  last_name: Cheung
  orcid: 0000-0001-8457-2572
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: Lora Beatrice Jaeger
  full_name: Sweeney, Lora Beatrice Jaeger
  id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
  last_name: Sweeney
  orcid: 0000-0001-9242-5601
citation:
  ama: Gobeil SA, Da Silveira Neto F, Silvestrelli G, et al. Lineage origin of spinal
    cord cell type diversity. <i>bioRxiv</i>. doi:<a href="https://doi.org/10.64898/2026.02.12.705305">10.64898/2026.02.12.705305</a>
  apa: Gobeil, S. A., Da Silveira Neto, F., Silvestrelli, G., Smits, M. G., Streicher,
    C., Cheung, G. T., … Sweeney, L. B. (n.d.). Lineage origin of spinal cord cell
    type diversity. <i>bioRxiv</i>. <a href="https://doi.org/10.64898/2026.02.12.705305">https://doi.org/10.64898/2026.02.12.705305</a>
  chicago: Gobeil, Sophie A, Francisco Da Silveira Neto, Giulia Silvestrelli, Matthijs
    Geert Smits, Carmen Streicher, Giselle T Cheung, Simon Hippenmeyer, and Lora B.
    Sweeney. “Lineage Origin of Spinal Cord Cell Type Diversity.” <i>BioRxiv</i>,
    n.d. <a href="https://doi.org/10.64898/2026.02.12.705305">https://doi.org/10.64898/2026.02.12.705305</a>.
  ieee: S. A. Gobeil <i>et al.</i>, “Lineage origin of spinal cord cell type diversity,”
    <i>bioRxiv</i>. .
  ista: Gobeil SA, Da Silveira Neto F, Silvestrelli G, Smits MG, Streicher C, Cheung
    GT, Hippenmeyer S, Sweeney LB. Lineage origin of spinal cord cell type diversity.
    bioRxiv, <a href="https://doi.org/10.64898/2026.02.12.705305">10.64898/2026.02.12.705305</a>.
  mla: Gobeil, Sophie A., et al. “Lineage Origin of Spinal Cord Cell Type Diversity.”
    <i>BioRxiv</i>, doi:<a href="https://doi.org/10.64898/2026.02.12.705305">10.64898/2026.02.12.705305</a>.
  short: S.A. Gobeil, F. Da Silveira Neto, G. Silvestrelli, M.G. Smits, C. Streicher,
    G.T. Cheung, S. Hippenmeyer, L.B. Sweeney, BioRxiv (n.d.).
corr_author: '1'
date_created: 2026-02-17T11:36:20Z
date_published: 2026-02-16T00:00:00Z
date_updated: 2026-04-14T08:16:55Z
day: '16'
ddc:
- '570'
department:
- _id: SiHi
- _id: LoSw
doi: 10.64898/2026.02.12.705305
fulldoi: https://doi.org/10.64898/2026.02.12.705305
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
main_file_link:
- open_access: '1'
  url: https://doi.org/10.64898/2026.02.12.705305
month: '02'
oa: 1
oa_version: Preprint
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
  grant_number: '101041551'
  name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
  grant_number: F7814
  name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
    transition: cell type to connection diversity'
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
  grant_number: F7805
  name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
    Mechanisms of Neural Stem Cell Lineage Progression
publication: bioRxiv
publication_status: submitted
status: public
title: Lineage origin of spinal cord cell type diversity
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19366'
abstract:
- lang: eng
  text: Staphylococcus aureus (S. aureus) is one of the most common causative agents
    of mammary gland infection and mastitis, but the specific role of S. aureus-derived
    extracellular vesicles (SaEVs) in mastitis has been poorly studied to date. Here,
    we aimed to investigate the response of bovine monocyte-derived macrophages (boMdM)
    to SaEVs of the genotype B (GTB) mastitis-related strain M5512B. Specifically,
    we evaluated the effects on the actin cytoskeleton, gene expression, and the SaEV
    proteomic cargo. Furthermore, we assessed to what extent the cellular and molecular
    response of boMdM to SaEVs differed from peripheral mononuclear blood cells (PBMCs)
    used for in vitro derivation of the former. We observed that SaEVs induced morphological
    changes in boMdM, leading to a pro-inflammatory and pyroptosis-related increased
    gene expression. Additionally, our study revealed that boMdM and PBMCs exhibited
    stimulus-specific differing responses. The proteomic analysis of SaEVs identified
    clusters of proteins related to virulence and antibiotic resistance, supporting
    the theory that S. aureus might use EVs to evade host defences and colonize the
    mammary gland. Our results bring new insights into how SaEVs might impact the
    host during an S. aureus infection, which can be useful for future S. aureus vaccine
    development.
acknowledgement: "The authors thank Michele Guastalla for his contributions to the
  boMdM analyses and Stephan Handschin from the Scientific Center for Optical and
  Electron Microscopy (ScopeM) of ETH Zurich for the TEM imaging. We gratefully acknowledge
  the Functional Genomics Center Zurich (FGCZ) for performing the mass spectrometry
  analysis for this study.\r\nOpen access funding provided by Swiss Federal Institute
  of Technology Zurich. This work was supported by basic funding from ETH Zurich."
article_processing_charge: Yes
article_type: original
author:
- first_name: Mara D.
  full_name: Saenz-De-Juano, Mara D.
  last_name: Saenz-De-Juano
- first_name: Giulia
  full_name: Silvestrelli, Giulia
  id: 12632ae8-799e-11ef-94a2-e5a3b5ef49e9
  last_name: Silvestrelli
- first_name: Samuel
  full_name: Buri, Samuel
  last_name: Buri
- first_name: Léa V.
  full_name: Zinsli, Léa V.
  last_name: Zinsli
- first_name: Mathias
  full_name: Schmelcher, Mathias
  last_name: Schmelcher
- first_name: Susanne E.
  full_name: Ulbrich, Susanne E.
  last_name: Ulbrich
citation:
  ama: Saenz-De-Juano MD, Silvestrelli G, Buri S, Zinsli LV, Schmelcher M, Ulbrich
    SE. Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce
    a pro-inflammatory response in bovine monocyte-derived macrophages. <i>Scientific
    Reports</i>. 2025;15:6059. doi:<a href="https://doi.org/10.1038/s41598-025-90466-6">10.1038/s41598-025-90466-6</a>
  apa: Saenz-De-Juano, M. D., Silvestrelli, G., Buri, S., Zinsli, L. V., Schmelcher,
    M., &#38; Ulbrich, S. E. (2025). Mastitis-related Staphylococcus aureus-derived
    extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived
    macrophages. <i>Scientific Reports</i>. Springer Nature. <a href="https://doi.org/10.1038/s41598-025-90466-6">https://doi.org/10.1038/s41598-025-90466-6</a>
  chicago: Saenz-De-Juano, Mara D., Giulia Silvestrelli, Samuel Buri, Léa V. Zinsli,
    Mathias Schmelcher, and Susanne E. Ulbrich. “Mastitis-Related Staphylococcus Aureus-Derived
    Extracellular Vesicles Induce a pro-Inflammatory Response in Bovine Monocyte-Derived
    Macrophages.” <i>Scientific Reports</i>. Springer Nature, 2025. <a href="https://doi.org/10.1038/s41598-025-90466-6">https://doi.org/10.1038/s41598-025-90466-6</a>.
  ieee: M. D. Saenz-De-Juano, G. Silvestrelli, S. Buri, L. V. Zinsli, M. Schmelcher,
    and S. E. Ulbrich, “Mastitis-related Staphylococcus aureus-derived extracellular
    vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages,”
    <i>Scientific Reports</i>, vol. 15. Springer Nature, p. 6059, 2025.
  ista: Saenz-De-Juano MD, Silvestrelli G, Buri S, Zinsli LV, Schmelcher M, Ulbrich
    SE. 2025. Mastitis-related Staphylococcus aureus-derived extracellular vesicles
    induce a pro-inflammatory response in bovine monocyte-derived macrophages. Scientific
    Reports. 15, 6059.
  mla: Saenz-De-Juano, Mara D., et al. “Mastitis-Related Staphylococcus Aureus-Derived
    Extracellular Vesicles Induce a pro-Inflammatory Response in Bovine Monocyte-Derived
    Macrophages.” <i>Scientific Reports</i>, vol. 15, Springer Nature, 2025, p. 6059,
    doi:<a href="https://doi.org/10.1038/s41598-025-90466-6">10.1038/s41598-025-90466-6</a>.
  short: M.D. Saenz-De-Juano, G. Silvestrelli, S. Buri, L.V. Zinsli, M. Schmelcher,
    S.E. Ulbrich, Scientific Reports 15 (2025) 6059.
date_created: 2025-03-09T23:01:26Z
date_published: 2025-02-19T00:00:00Z
date_updated: 2025-09-30T10:58:59Z
day: '19'
ddc:
- '570'
department:
- _id: LoSw
doi: 10.1038/s41598-025-90466-6
external_id:
  isi:
  - '001426697000031'
  pmid:
  - '39972051'
file:
- access_level: open_access
  checksum: 51b55ae299de1fa126016a11024b499a
  content_type: application/pdf
  creator: dernst
  date_created: 2025-03-10T12:00:34Z
  date_updated: 2025-03-10T12:00:34Z
  file_id: '19380'
  file_name: 2025_ScientificReports_SaenzdeJuano.pdf
  file_size: 2780316
  relation: main_file
  success: 1
file_date_updated: 2025-03-10T12:00:34Z
fulldoi: https://doi.org/10.1038/s41598-025-90466-6
has_accepted_license: '1'
intvolume: '        15'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: '6059'
pmid: 1
publication: Scientific Reports
publication_identifier:
  eissn:
  - 2045-2322
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce
  a pro-inflammatory response in bovine monocyte-derived macrophages
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 15
year: '2025'
...