[{"article_processing_charge":"No","publisher":"American Chemical Society","doi":"10.1021/acs.biomac.6c00345","oa_version":"Preprint","abstract":[{"lang":"eng","text":"The collagen triple helix assembles hierarchically into bundled oligomers, solvated networks, and fibers. Synthetic peptide assemblies, driven by supramolecular interactions, can form single triple helices through intrahelical amino acid pairs; however, the principles guiding interhelical associations into higher-order structures remain unclear. Here, we incorporate cation−π and electrostatic charge pairs to probe interhelical interactions and elucidate the mechanisms driving triple helix assembly into fibrils, nanotubes, and nanosheets. Introducing cation−π pairs into a fibrillating collagen mimetic resulted in D-periodic fibrils with pH-sensitive gelation. By alternating the presentation of electrostatic and cation−π pairs, the assembly of another D-periodic fibril featuring inner and outer triple-helical layers was resolved by cryo electron microscopy to a resolution of 8 Å. At physiological pH, antiparallel association of these triple helices leads to the formation of nanotubes. The packing behavior of triple helices correlates with the interhelical interactions, where parallel associations favor fibril formation and antiparallel interactions drive nanotube and nanosheet assembly. These self-assembling triple-helical peptides demonstrate how packing of higher-order structures can be tailored with supramolecular interactions and establish the relationship of different hierarchical collagen-mimetic assemblies as pH-dependent."}],"OA_type":"green","date_published":"2026-04-13T00:00:00Z","year":"2026","intvolume":"        27","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.02.15.637692"}],"author":[{"first_name":"Carson C.","last_name":"Cole","full_name":"Cole, Carson C."},{"first_name":"Mark A.B.","last_name":"Kreutzberger","full_name":"Kreutzberger, Mark A.B."},{"full_name":"Klein, Kevin","last_name":"Klein","id":"1e7ede04-9e54-11f0-9ec4-8d4d5563c398","first_name":"Kevin"},{"first_name":"Kiana A.","full_name":"Cahue, Kiana A.","last_name":"Cahue"},{"last_name":"Pogostin","full_name":"Pogostin, Brett H.","first_name":"Brett H."},{"first_name":"Adam C.","full_name":"Farsheed, Adam C.","last_name":"Farsheed"},{"last_name":"Swain","full_name":"Swain, Joseph W.R.","first_name":"Joseph W.R."},{"full_name":"Bui, Thi H.","last_name":"Bui","first_name":"Thi H."},{"last_name":"Dey","full_name":"Dey, Arghadip","first_name":"Arghadip"},{"first_name":"Jonathan T.","full_name":"Makhoul, Jonathan T.","last_name":"Makhoul"},{"first_name":"Marija","last_name":"Dubackic","full_name":"Dubackic, Marija"},{"first_name":"Antara","last_name":"Pal","full_name":"Pal, Antara"},{"first_name":"Ulf","full_name":"Olsson, Ulf","last_name":"Olsson"},{"orcid":"0000-0002-7854-2139","first_name":"Anđela","full_name":"Šarić, Anđela","last_name":"Šarić","id":"bf63d406-f056-11eb-b41d-f263a6566d8b"},{"full_name":"Egelman, Edward H.","last_name":"Egelman","first_name":"Edward H."},{"full_name":"Hartgerink, Jeffrey D.","last_name":"Hartgerink","first_name":"Jeffrey D."}],"citation":{"chicago":"Cole, Carson C., Mark A.B. Kreutzberger, Kevin Klein, Kiana A. Cahue, Brett H. Pogostin, Adam C. Farsheed, Joseph W.R. Swain, et al. “Supramolecular Assembly of Collagen-Mimetic Eptide D-Periodic Fibrils and Nanoassemblies.” <i>Biomacromolecules</i>. American Chemical Society, 2026. <a href=\"https://doi.org/10.1021/acs.biomac.6c00345\">https://doi.org/10.1021/acs.biomac.6c00345</a>.","short":"C.C. Cole, M.A.B. Kreutzberger, K. Klein, K.A. Cahue, B.H. Pogostin, A.C. Farsheed, J.W.R. Swain, T.H. Bui, A. Dey, J.T. Makhoul, M. Dubackic, A. Pal, U. Olsson, A. Šarić, E.H. Egelman, J.D. Hartgerink, Biomacromolecules 27 (2026) 2956–2965.","ista":"Cole CC, Kreutzberger MAB, Klein K, Cahue KA, Pogostin BH, Farsheed AC, Swain JWR, Bui TH, Dey A, Makhoul JT, Dubackic M, Pal A, Olsson U, Šarić A, Egelman EH, Hartgerink JD. 2026. Supramolecular assembly of collagen-mimetic eptide D-periodic fibrils and nanoassemblies. Biomacromolecules. 27(4), 2956–2965.","apa":"Cole, C. C., Kreutzberger, M. A. B., Klein, K., Cahue, K. A., Pogostin, B. H., Farsheed, A. C., … Hartgerink, J. D. (2026). Supramolecular assembly of collagen-mimetic eptide D-periodic fibrils and nanoassemblies. <i>Biomacromolecules</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.biomac.6c00345\">https://doi.org/10.1021/acs.biomac.6c00345</a>","ieee":"C. C. Cole <i>et al.</i>, “Supramolecular assembly of collagen-mimetic eptide D-periodic fibrils and nanoassemblies,” <i>Biomacromolecules</i>, vol. 27, no. 4. American Chemical Society, pp. 2956–2965, 2026.","mla":"Cole, Carson C., et al. “Supramolecular Assembly of Collagen-Mimetic Eptide D-Periodic Fibrils and Nanoassemblies.” <i>Biomacromolecules</i>, vol. 27, no. 4, American Chemical Society, 2026, pp. 2956–65, doi:<a href=\"https://doi.org/10.1021/acs.biomac.6c00345\">10.1021/acs.biomac.6c00345</a>.","ama":"Cole CC, Kreutzberger MAB, Klein K, et al. Supramolecular assembly of collagen-mimetic eptide D-periodic fibrils and nanoassemblies. <i>Biomacromolecules</i>. 2026;27(4):2956-2965. doi:<a href=\"https://doi.org/10.1021/acs.biomac.6c00345\">10.1021/acs.biomac.6c00345</a>"},"language":[{"iso":"eng"}],"status":"public","publication":"Biomacromolecules","title":"Supramolecular assembly of collagen-mimetic eptide D-periodic fibrils and nanoassemblies","_id":"21749","month":"04","scopus_import":"1","quality_controlled":"1","publication_identifier":{"eissn":["1526-4602"]},"acknowledgement":"The authors acknowledge Crispin Hetherington and L. Tracy Yu for their technical assistance and insights. This work was funded in part by the National Science Foundation (CHE 2203937), the National Science Foundation Graduate Research Fellowship (Grant No. 1842494), the Welch Foundation (C-2141), the Swedish Research Council (2020-04633), and the NIH (GM122510). This work benefited from using the SasView application, originally developed under NSF award DMR-0520547. SasView contains code developed with funding from the European Union’s Horizon 2020 research and innovation program under the SINE2020 project, Grant Agreement No. 654000. This work was partly done using the Shared Equipment Authority resources at Rice University.","OA_place":"repository","page":"2956-2965","oa":1,"volume":27,"issue":"4","publication_status":"published","department":[{"_id":"AnSa"}],"article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"13","date_created":"2026-04-19T22:07:46Z","date_updated":"2026-05-06T05:43:44Z","type":"journal_article"},{"publication":"ACS Nano","title":"In operando imaging electrostatic-driven disassembly and reassembly of collagen nanostructures","file_date_updated":"2025-01-09T12:06:48Z","_id":"17239","scopus_import":"1","month":"07","quality_controlled":"1","external_id":{"isi":["001263155500001"],"pmid":["38958189"]},"publication_identifier":{"eissn":["1936-086X"],"issn":["1936-0851"]},"acknowledgement":"We are grateful to Nancy Forde (Simon Fraser University) for her motivating comments. Financial support from the Ministerio de Ciencia, Innovación y Universidades (PID2019-106801GB-I00 and PID2022-136851NB-I00) is acknowledged. A.Š. and K.K. acknowledge support from the Royal Society University Research Fellowship and ERC the European Union’s Horizon 2020584 Research and Innovation Programme (Grant No. 585 80296).","OA_place":"publisher","page":"18485-18492","oa":1,"volume":18,"publication_status":"published","issue":"28","department":[{"_id":"AnSa"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","day":"16","date_created":"2024-07-14T22:01:12Z","project":[{"call_identifier":"H2020","grant_number":"802960","_id":"eba2549b-77a9-11ec-83b8-a81e493eae4e","name":"Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines"}],"date_updated":"2025-12-16T09:01:10Z","isi":1,"type":"journal_article","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"article_processing_charge":"Yes (in subscription journal)","publisher":"American Chemical Society","ddc":["540"],"doi":"10.1021/acsnano.4c03839","oa_version":"Published Version","file":[{"date_updated":"2025-01-09T12:06:48Z","file_size":10036838,"file_id":"18808","creator":"dernst","content_type":"application/pdf","success":1,"checksum":"b7e9ce718e92f568bcb3810e8e28e458","access_level":"open_access","relation":"main_file","date_created":"2025-01-09T12:06:48Z","file_name":"2024_ACSNano_GarciaSacristan.pdf"}],"abstract":[{"text":"Collagen is the most abundant protein in tissue scaffolds in live organisms. Collagen can self-assemble in vitro, which has led to a number of biotechnological and biomedical applications. To understand the dominant factors that participate in the formation of collagen nanostructures, here we study in real time and with nanoscale resolution the disassembly and reassembly of collagens. We implement a high-speed force microscope, which provides in situ high spatiotemporal resolution images of collagen nanostructures under changing pH conditions. The disassembly and reassembly are dominated by the electrostatic interactions among amino-acid residues of different molecules. Acidic conditions favor disassembly by neutralizing negatively charged residues. The process sets a net repulsive force between collagen molecules. A neutral pH favors the presence of negative and positively charged residues along the collagen molecules, which promotes their electrostatic attraction. Molecular dynamics simulations reproduce the experimental behavior and validate the electrostatic-based model of the disassembly and reassembly processes.","lang":"eng"}],"pmid":1,"OA_type":"hybrid","date_published":"2024-07-16T00:00:00Z","year":"2024","intvolume":"        18","author":[{"full_name":"Garcia-Sacristan, Clara","last_name":"Garcia-Sacristan","first_name":"Clara"},{"first_name":"Victor G.","full_name":"Gisbert, Victor G.","last_name":"Gisbert"},{"last_name":"Klein","full_name":"Klein, Kevin","id":"1e7ede04-9e54-11f0-9ec4-8d4d5563c398","first_name":"Kevin"},{"full_name":"Šarić, Anđela","last_name":"Šarić","id":"bf63d406-f056-11eb-b41d-f263a6566d8b","orcid":"0000-0002-7854-2139","first_name":"Anđela"},{"full_name":"Garcia, Ricardo","last_name":"Garcia","first_name":"Ricardo"}],"citation":{"ista":"Garcia-Sacristan C, Gisbert VG, Klein K, Šarić A, Garcia R. 2024. In operando imaging electrostatic-driven disassembly and reassembly of collagen nanostructures. ACS Nano. 18(28), 18485–18492.","short":"C. Garcia-Sacristan, V.G. Gisbert, K. Klein, A. Šarić, R. Garcia, ACS Nano 18 (2024) 18485–18492.","chicago":"Garcia-Sacristan, Clara, Victor G. Gisbert, Kevin Klein, Anđela Šarić, and Ricardo Garcia. “In Operando Imaging Electrostatic-Driven Disassembly and Reassembly of Collagen Nanostructures.” <i>ACS Nano</i>. American Chemical Society, 2024. <a href=\"https://doi.org/10.1021/acsnano.4c03839\">https://doi.org/10.1021/acsnano.4c03839</a>.","ama":"Garcia-Sacristan C, Gisbert VG, Klein K, Šarić A, Garcia R. In operando imaging electrostatic-driven disassembly and reassembly of collagen nanostructures. <i>ACS Nano</i>. 2024;18(28):18485-18492. doi:<a href=\"https://doi.org/10.1021/acsnano.4c03839\">10.1021/acsnano.4c03839</a>","mla":"Garcia-Sacristan, Clara, et al. “In Operando Imaging Electrostatic-Driven Disassembly and Reassembly of Collagen Nanostructures.” <i>ACS Nano</i>, vol. 18, no. 28, American Chemical Society, 2024, pp. 18485–92, doi:<a href=\"https://doi.org/10.1021/acsnano.4c03839\">10.1021/acsnano.4c03839</a>.","apa":"Garcia-Sacristan, C., Gisbert, V. G., Klein, K., Šarić, A., &#38; Garcia, R. (2024). In operando imaging electrostatic-driven disassembly and reassembly of collagen nanostructures. <i>ACS Nano</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acsnano.4c03839\">https://doi.org/10.1021/acsnano.4c03839</a>","ieee":"C. Garcia-Sacristan, V. G. Gisbert, K. Klein, A. Šarić, and R. Garcia, “In operando imaging electrostatic-driven disassembly and reassembly of collagen nanostructures,” <i>ACS Nano</i>, vol. 18, no. 28. American Chemical Society, pp. 18485–18492, 2024."},"ec_funded":1,"language":[{"iso":"eng"}],"status":"public","has_accepted_license":"1"}]