---
OA_place: repository
OA_type: green
_id: '21291'
abstract:
- lang: eng
text: The complexity and specificity of movement in vertebrates is driven by a rich
diversity of spinal motor and interneuron cell types. During development, eleven
spinal cord progenitor domains generate an equivalent number of cardinal neuron
types. How progenitor domains, individual progenitors, and post-mitotic diversity
relate is still unknown. We performed high-resolution, single-progenitor cell
lineage tracing in the embryonic mouse spinal cord using mosaic analysis with
double markers (MADM). Our quantitative study of lineage progression revealed
that spinal cord progenitors undergo highly variable numbers of proliferative,
neurogenic, and gliogenic cell divisions. The nascent clonally-related neurons
migrate radially over large distances, span the dorsoventral axis, and even cross
the midline, demonstrating striking bilaterality. Molecular and morphometric analysis
indicate high levels of progenitor multipotency, with an individual progenitor
capable of producing several molecularly and morphologically distinct neuron types,
as well as astrocytes. These findings redefine spinal cord development as a process
in which lineage variability — rather than rigid progenitor identity — drives
the generation of cellular diversity.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: "We would like to thank Elizabeth Marin, Anna Kicheva, Igor Adameyko,
and James Briscoe as\r\nwell as members of the Sweeney and Hippemeyer labs and SFB
consortium for comments on\r\nthe manuscript. We are also grateful for the technical
support of the Preclinical and Imaging and\r\nOptics Facilities support teams (ISTA).
In addition, we thank our funding sources for providing\r\nthe resources to do these
experiments: Horizon Europe ERC Starting Grant Number 101041551\r\n(M.S.; L.B.S.);
Special Research Program (SFB) of the Austrian Science Fund (FWF)\r\nNeuroStem Modulation
Project numbers F7814-B (S.A.G.; M.S.; G.S.; and L.B.S.) and F7805\r\n(G.C. and
S.H.). S.A.G is supported by a Boehringer Ingelheim Fonds PhD Fellowship, F.D.S.N.\r\nby
an Institute of Science and Technology Austria (ISTA) GROW fellowship, and G.C.
by an\r\nISTA Plus postdoctoral fellowship from the European Commission. S.H./L.B.S.
and G.C. were\r\nadditionally supported by institutional funds from the ISTA and
the University of Exeter,\r\nrespectively. "
article_processing_charge: No
author:
- first_name: Sophie A
full_name: Gobeil, Sophie A
id: 2f3e9efb-eb24-11ec-86b2-88efb11d59fa
last_name: Gobeil
- first_name: Francisco
full_name: Da Silveira Neto, Francisco
id: 8cfb7412-10a7-11f1-add1-82b44e6418f2
last_name: Da Silveira Neto
- first_name: Giulia
full_name: Silvestrelli, Giulia
id: 12632ae8-799e-11ef-94a2-e5a3b5ef49e9
last_name: Silvestrelli
- first_name: Matthijs Geert
full_name: Smits, Matthijs Geert
id: 7a231d52-e216-11ee-a0bb-8acd55f8f1f0
last_name: Smits
- first_name: Carmen
full_name: Streicher, Carmen
id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
last_name: Streicher
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Lora Beatrice Jaeger
full_name: Sweeney, Lora Beatrice Jaeger
id: 56BE8254-C4F0-11E9-8E45-0B23E6697425
last_name: Sweeney
orcid: 0000-0001-9242-5601
citation:
ama: Gobeil SA, Da Silveira Neto F, Silvestrelli G, et al. Lineage origin of spinal
cord cell type diversity. bioRxiv. doi:10.64898/2026.02.12.705305
apa: Gobeil, S. A., Da Silveira Neto, F., Silvestrelli, G., Smits, M. G., Streicher,
C., Cheung, G. T., … Sweeney, L. B. (n.d.). Lineage origin of spinal cord cell
type diversity. bioRxiv. https://doi.org/10.64898/2026.02.12.705305
chicago: Gobeil, Sophie A, Francisco Da Silveira Neto, Giulia Silvestrelli, Matthijs
Geert Smits, Carmen Streicher, Giselle T Cheung, Simon Hippenmeyer, and Lora B.
Sweeney. “Lineage Origin of Spinal Cord Cell Type Diversity.” BioRxiv,
n.d. https://doi.org/10.64898/2026.02.12.705305.
ieee: S. A. Gobeil et al., “Lineage origin of spinal cord cell type diversity,”
bioRxiv. .
ista: Gobeil SA, Da Silveira Neto F, Silvestrelli G, Smits MG, Streicher C, Cheung
GT, Hippenmeyer S, Sweeney LB. Lineage origin of spinal cord cell type diversity.
bioRxiv, 10.64898/2026.02.12.705305.
mla: Gobeil, Sophie A., et al. “Lineage Origin of Spinal Cord Cell Type Diversity.”
BioRxiv, doi:10.64898/2026.02.12.705305.
short: S.A. Gobeil, F. Da Silveira Neto, G. Silvestrelli, M.G. Smits, C. Streicher,
G.T. Cheung, S. Hippenmeyer, L.B. Sweeney, BioRxiv (n.d.).
corr_author: '1'
date_created: 2026-02-17T11:36:20Z
date_published: 2026-02-16T00:00:00Z
date_updated: 2026-04-14T08:16:55Z
day: '16'
ddc:
- '570'
department:
- _id: SiHi
- _id: LoSw
doi: 10.64898/2026.02.12.705305
fulldoi: https://doi.org/10.64898/2026.02.12.705305
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.64898/2026.02.12.705305
month: '02'
oa: 1
oa_version: Preprint
project:
- _id: ebb66355-77a9-11ec-83b8-b8ac210a4dae
grant_number: '101041551'
name: Development and Evolution of Tetrapod Motor Circuits
- _id: 8da85f50-16d5-11f0-9cad-eab8b0ff6c9e
grant_number: F7814
name: 'Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb
transition: cell type to connection diversity'
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: bioRxiv
publication_status: submitted
status: public
title: Lineage origin of spinal cord cell type diversity
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...