---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19404'
abstract:
- lang: eng
  text: Cell migration is a fundamental process during embryonic development. Most
    studies in vivo have focused on the migration of cells using the extracellular
    matrix (ECM) as their substrate for migration. In contrast, much less is known
    about how cells migrate on other cells, as found in early embryos when the ECM
    has not yet formed. Here, we show that lateral mesendoderm (LME) cells in the
    early zebrafish gastrula use the ectoderm as their substrate for migration. We
    show that the lateral ectoderm is permissive for the animal-pole-directed migration
    of LME cells, while the ectoderm at the animal pole halts it. These differences
    in permissiveness depend on the lateral ectoderm being more cohesive than the
    animal ectoderm, a property controlled by bone morphogenetic protein (BMP) signaling
    within the ectoderm. Collectively, these findings identify ectoderm tissue cohesion
    as one critical factor in regulating LME migration during zebrafish gastrulation.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: ScienComp
acknowledgement: 'We are grateful to the colleagues who contributed to this work with
  discussions, technical advice, and feedback on the manuscript: Irene Steccari, David
  Labrousse Arias and the other members of the Heisenberg lab, Nicole Amberg, Florian
  Pauler, Nicoletta Petridou, Elena Scarpa, and Edouard Hannezo. We also thank the
  Imaging and Optics Facility, the Life Science Facility, and the Scientific Computing
  Unit at ISTA for support. The Next Generation Sequencing Facility at Vienna BioCenter
  Core Facilities performed the RNA-seq for animal and lateral ectoderm. D.B.B. was
  supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral
  Fellowship (ALTF 343-2022). S. Tavano was supported by an EMBO Postdoctoral Fellowship
  (ALTF 1159-2018).'
article_number: '115387'
article_processing_charge: Yes
article_type: original
author:
- first_name: Ste
  full_name: Tavano, Ste
  id: 2F162F0C-F248-11E8-B48F-1D18A9856A87
  last_name: Tavano
  orcid: 0000-0001-9970-7804
- first_name: David
  full_name: Brückner, David
  id: e1e86031-6537-11eb-953a-f7ab92be508d
  last_name: Brückner
  orcid: 0000-0001-7205-2975
- first_name: Saren
  full_name: Tasciyan, Saren
  id: 4323B49C-F248-11E8-B48F-1D18A9856A87
  last_name: Tasciyan
  orcid: 0000-0003-1671-393X
- first_name: Xin
  full_name: Tong, Xin
  id: 50F65CDC-AA30-11E9-A72B-8A12E6697425
  last_name: Tong
- first_name: Roland
  full_name: Kardos, Roland
  id: 4039350E-F248-11E8-B48F-1D18A9856A87
  last_name: Kardos
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Tavano S, Brückner D, Tasciyan S, et al. BMP-dependent patterning of ectoderm
    tissue material properties modulates lateral mesendoderm cell migration during
    early zebrafish gastrulation. <i>Cell Reports</i>. 2025;44(3). doi:<a href="https://doi.org/10.1016/j.celrep.2025.115387">10.1016/j.celrep.2025.115387</a>
  apa: Tavano, S., Brückner, D., Tasciyan, S., Tong, X., Kardos, R., Schauer, A.,
    … Heisenberg, C.-P. J. (2025). BMP-dependent patterning of ectoderm tissue material
    properties modulates lateral mesendoderm cell migration during early zebrafish
    gastrulation. <i>Cell Reports</i>. Elsevier. <a href="https://doi.org/10.1016/j.celrep.2025.115387">https://doi.org/10.1016/j.celrep.2025.115387</a>
  chicago: Tavano, Ste, David Brückner, Saren Tasciyan, Xin Tong, Roland Kardos, Alexandra
    Schauer, Robert Hauschild, and Carl-Philipp J Heisenberg. “BMP-Dependent Patterning
    of Ectoderm Tissue Material Properties Modulates Lateral Mesendoderm Cell Migration
    during Early Zebrafish Gastrulation.” <i>Cell Reports</i>. Elsevier, 2025. <a
    href="https://doi.org/10.1016/j.celrep.2025.115387">https://doi.org/10.1016/j.celrep.2025.115387</a>.
  ieee: S. Tavano <i>et al.</i>, “BMP-dependent patterning of ectoderm tissue material
    properties modulates lateral mesendoderm cell migration during early zebrafish
    gastrulation,” <i>Cell Reports</i>, vol. 44, no. 3. Elsevier, 2025.
  ista: Tavano S, Brückner D, Tasciyan S, Tong X, Kardos R, Schauer A, Hauschild R,
    Heisenberg C-PJ. 2025. BMP-dependent patterning of ectoderm tissue material properties
    modulates lateral mesendoderm cell migration during early zebrafish gastrulation.
    Cell Reports. 44(3), 115387.
  mla: Tavano, Ste, et al. “BMP-Dependent Patterning of Ectoderm Tissue Material Properties
    Modulates Lateral Mesendoderm Cell Migration during Early Zebrafish Gastrulation.”
    <i>Cell Reports</i>, vol. 44, no. 3, 115387, Elsevier, 2025, doi:<a href="https://doi.org/10.1016/j.celrep.2025.115387">10.1016/j.celrep.2025.115387</a>.
  short: S. Tavano, D. Brückner, S. Tasciyan, X. Tong, R. Kardos, A. Schauer, R. Hauschild,
    C.-P.J. Heisenberg, Cell Reports 44 (2025).
corr_author: '1'
date_created: 2025-03-16T23:01:24Z
date_published: 2025-03-25T00:00:00Z
date_updated: 2025-10-22T07:00:04Z
day: '25'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: MiSi
- _id: Bio
doi: 10.1016/j.celrep.2025.115387
external_id:
  isi:
  - '001443652700001'
  pmid:
  - '40057955'
file:
- access_level: open_access
  checksum: 57e05dd1598c807af0afdb32cec039d3
  content_type: application/pdf
  creator: dernst
  date_created: 2025-03-17T10:26:54Z
  date_updated: 2025-03-17T10:26:54Z
  file_id: '19413'
  file_name: 2025_CellReports_Tavano.pdf
  file_size: 9067797
  relation: main_file
  success: 1
file_date_updated: 2025-03-17T10:26:54Z
has_accepted_license: '1'
intvolume: '        44'
isi: 1
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 34e2a5b5-11ca-11ed-8bc3-b2265616ef0b
  grant_number: ALTF 343-2022
  name: A mechano-chemical theory for stem cell fate decisions in organoid development
- _id: 269CD5C4-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 1159-2018
  name: 'Mechanosensation in cell migration: the role of friction forces in cell polarization
    and directed migration'
publication: Cell Reports
publication_identifier:
  eissn:
  - 2211-1247
  issn:
  - 2639-1856
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: BMP-dependent patterning of ectoderm tissue material properties modulates lateral
  mesendoderm cell migration during early zebrafish gastrulation
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2025'
...
---
_id: '15048'
abstract:
- lang: eng
  text: Embryogenesis results from the coordinated activities of different signaling
    pathways controlling cell fate specification and morphogenesis. In vertebrate
    gastrulation, both Nodal and BMP signaling play key roles in germ layer specification
    and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis
    is still insufficiently understood. Here, we took a reductionist approach using
    zebrafish embryonic explants to study the coordination of Nodal and BMP signaling
    for embryo patterning and morphogenesis. We show that Nodal signaling triggers
    explant elongation by inducing mesendodermal progenitors but also suppressing
    BMP signaling activity at the site of mesendoderm induction. Consistent with this,
    ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm
    intercalations, key processes during explant elongation. Translating these ex
    vivo observations to the intact embryo showed that, similar to explants, Nodal
    signaling suppresses the effect of BMP signaling on cell intercalations in the
    dorsal domain, thus allowing robust embryonic axis elongation. These findings
    suggest a dual function of Nodal signaling in embryonic axis elongation by both
    inducing mesendoderm and suppressing BMP effects in the dorsal portion of the
    mesendoderm.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Patrick Müller for sharing the chordintt250 mutant zebrafish
  line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b
  plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated
  the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro
  and Katherine Rogers and members of the Heisenberg lab for discussions, technical
  advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo
  for discussions. We thank the Imaging and Optics Facility as well as the Life Science
  facility at IST Austria for support with microscopy and fish maintenance.\r\nThis
  work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573
  to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy
  of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience
  and Technology Austria. "
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: Kornelija
  full_name: Pranjic-Ferscha, Kornelija
  id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
  last_name: Pranjic-Ferscha
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation
    by Nodal-dependent restriction of BMP signaling. <i>Development</i>. 2024;151(4):1-18.
    doi:<a href="https://doi.org/10.1242/dev.202316">10.1242/dev.202316</a>
  apa: Schauer, A., Pranjic-Ferscha, K., Hauschild, R., &#38; Heisenberg, C.-P. J.
    (2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling.
    <i>Development</i>. The Company of Biologists. <a href="https://doi.org/10.1242/dev.202316">https://doi.org/10.1242/dev.202316</a>
  chicago: Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp
    J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.”
    <i>Development</i>. The Company of Biologists, 2024. <a href="https://doi.org/10.1242/dev.202316">https://doi.org/10.1242/dev.202316</a>.
  ieee: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust
    axis elongation by Nodal-dependent restriction of BMP signaling,” <i>Development</i>,
    vol. 151, no. 4. The Company of Biologists, pp. 1–18, 2024.
  ista: Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis
    elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4),
    1–18.
  mla: Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction
    of BMP Signaling.” <i>Development</i>, vol. 151, no. 4, The Company of Biologists,
    2024, pp. 1–18, doi:<a href="https://doi.org/10.1242/dev.202316">10.1242/dev.202316</a>.
  short: A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development
    151 (2024) 1–18.
corr_author: '1'
date_created: 2024-03-03T23:00:50Z
date_published: 2024-02-01T00:00:00Z
date_updated: 2025-09-04T12:10:40Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.1242/dev.202316
ec_funded: 1
external_id:
  isi:
  - '001170580200001'
  pmid:
  - '38372390'
file:
- access_level: open_access
  checksum: 6961ea10012bf0d266681f9628bb8f13
  content_type: application/pdf
  creator: dernst
  date_created: 2024-03-04T07:24:43Z
  date_updated: 2024-03-04T07:24:43Z
  file_id: '15050'
  file_name: 2024_Development_Schauer.pdf
  file_size: 14839986
  relation: main_file
  success: 1
file_date_updated: 2024-03-04T07:24:43Z
has_accepted_license: '1'
intvolume: '       151'
isi: 1
issue: '4'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '02'
oa: 1
oa_version: Published Version
page: 1-18
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
  grant_number: '25239'
  name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
publication: Development
publication_identifier:
  eissn:
  - 1477-9129
  issn:
  - 0950-1991
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
  record:
  - id: '14926'
    relation: research_data
    status: public
scopus_import: '1'
status: public
title: Robust axis elongation by Nodal-dependent restriction of BMP signaling
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 151
year: '2024'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18940'
abstract:
- lang: eng
  text: BMP signaling has a conserved function in patterning the dorsal-ventral body
    axis in Bilateria and the directive axis in anthozoan cnidarians. So far, cnidarian
    studies have focused on the role of different BMP signaling network components
    in regulating pSMAD1/5 gradient formation. Much less is known about the target
    genes downstream of BMP signaling. To address this, we generated a genome-wide
    list of direct pSMAD1/5 target genes in the anthozoan <jats:italic>Nematostella
    vectensis</jats:italic>, several of which were conserved in <jats:italic>Drosophila</jats:italic>
    and <jats:italic>Xenopus</jats:italic>. Our ChIP-seq analysis revealed that many
    of the regulatory molecules with documented bilaterally symmetric expression in
    <jats:italic>Nematostella</jats:italic> are directly controlled by BMP signaling.
    We identified several so far uncharacterized BMP-dependent transcription factors
    and signaling molecules, whose bilaterally symmetric expression may be indicative
    of their involvement in secondary axis patterning. One of these molecules is <jats:italic>zswim4-6</jats:italic>,
    which encodes a novel nuclear protein that can modulate the pSMAD1/5 gradient
    and potentially promote BMP-dependent gene repression.
acknowledgement: This work was funded by the Austrian Science Foundation (FWF) grants
  P26962-B21 and P32705-B to GG and by the European Research Council (ERC) under the
  European Union’s Horizon 2020 research and innovation program (grant agreement No
  637840 [QUANTPATTERN] and 863952 [ACE-OF-SPACE]) to PM. We thank Michaela Schwaiger,
  Taras Kreslavsky, Hiromi Tagoh, and Patricio Ferrer Murguia for their help with
  the ChIP protocol, Matthias Richter and Christian Hofer for their assistance with
  in situ analyses, Emilio Gonzalez Morales for making the measurements for Figure
  6—figure supplement 3, Catrin Weiler for the assistance in cloning zebrafish zswim5,
  David Mörsdorf for critically reading the manuscript and help with data visualization,
  and the Core Facility for Cell Imaging and Ultrastructure Research of the University
  of Vienna for access to the confocal microscope.
article_processing_charge: Yes
article_type: original
author:
- first_name: Paul
  full_name: Knabl, Paul
  last_name: Knabl
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: Autumn P
  full_name: Pomreinke, Autumn P
  last_name: Pomreinke
- first_name: Bob
  full_name: Zimmermann, Bob
  last_name: Zimmermann
- first_name: Katherine W
  full_name: Rogers, Katherine W
  last_name: Rogers
- first_name: Daniel
  full_name: Čapek, Daniel
  last_name: Čapek
- first_name: Patrick
  full_name: Müller, Patrick
  last_name: Müller
- first_name: Grigory
  full_name: Genikhovich, Grigory
  last_name: Genikhovich
citation:
  ama: Knabl P, Schauer A, Pomreinke AP, et al. Analysis of SMAD1/5 target genes in
    a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator. <i>eLife</i>.
    2024;13. doi:<a href="https://doi.org/10.7554/elife.80803">10.7554/elife.80803</a>
  apa: Knabl, P., Schauer, A., Pomreinke, A. P., Zimmermann, B., Rogers, K. W., Čapek,
    D., … Genikhovich, G. (2024). Analysis of SMAD1/5 target genes in a sea anemone
    reveals ZSWIM4-6 as a novel BMP signaling modulator. <i>ELife</i>. eLife Sciences
    Publications. <a href="https://doi.org/10.7554/elife.80803">https://doi.org/10.7554/elife.80803</a>
  chicago: Knabl, Paul, Alexandra Schauer, Autumn P Pomreinke, Bob Zimmermann, Katherine
    W Rogers, Daniel Čapek, Patrick Müller, and Grigory Genikhovich. “Analysis of
    SMAD1/5 Target Genes in a Sea Anemone Reveals ZSWIM4-6 as a Novel BMP Signaling
    Modulator.” <i>ELife</i>. eLife Sciences Publications, 2024. <a href="https://doi.org/10.7554/elife.80803">https://doi.org/10.7554/elife.80803</a>.
  ieee: P. Knabl <i>et al.</i>, “Analysis of SMAD1/5 target genes in a sea anemone
    reveals ZSWIM4-6 as a novel BMP signaling modulator,” <i>eLife</i>, vol. 13. eLife
    Sciences Publications, 2024.
  ista: Knabl P, Schauer A, Pomreinke AP, Zimmermann B, Rogers KW, Čapek D, Müller
    P, Genikhovich G. 2024. Analysis of SMAD1/5 target genes in a sea anemone reveals
    ZSWIM4-6 as a novel BMP signaling modulator. eLife. 13.
  mla: Knabl, Paul, et al. “Analysis of SMAD1/5 Target Genes in a Sea Anemone Reveals
    ZSWIM4-6 as a Novel BMP Signaling Modulator.” <i>ELife</i>, vol. 13, eLife Sciences
    Publications, 2024, doi:<a href="https://doi.org/10.7554/elife.80803">10.7554/elife.80803</a>.
  short: P. Knabl, A. Schauer, A.P. Pomreinke, B. Zimmermann, K.W. Rogers, D. Čapek,
    P. Müller, G. Genikhovich, ELife 13 (2024).
date_created: 2025-01-29T08:48:34Z
date_published: 2024-02-07T00:00:00Z
date_updated: 2025-01-29T08:56:21Z
day: '07'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.7554/elife.80803
file:
- access_level: open_access
  checksum: 24548a184215d3f4547bba535ccfd7b1
  content_type: application/pdf
  creator: dernst
  date_created: 2025-01-29T08:50:18Z
  date_updated: 2025-01-29T08:50:18Z
  file_id: '18941'
  file_name: 2024_eLife_Knabl.pdf
  file_size: 11855972
  relation: main_file
  success: 1
file_date_updated: 2025-01-29T08:50:18Z
has_accepted_license: '1'
intvolume: '        13'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
publication: eLife
publication_identifier:
  issn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel
  BMP signaling modulator
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2024'
...
---
_id: '12891'
abstract:
- lang: eng
  text: "The tight spatiotemporal coordination of signaling activity determining embryo\r\npatterning
    and the physical processes driving embryo morphogenesis renders\r\nembryonic development
    robust, such that key developmental processes can unfold\r\nrelatively normally
    even outside of the full embryonic context. For instance, embryonic\r\nstem cell
    cultures can recapitulate the hallmarks of gastrulation, i.e. break symmetry\r\nleading
    to germ layer formation and morphogenesis, in a very reduced environment.\r\nThis
    leads to questions on specific contributions of embryo-specific features, such
    as\r\nthe presence of extraembryonic tissues, which are inherently involved in
    gastrulation\r\nin the full embryonic context. To address this, we established
    zebrafish embryonic\r\nexplants without the extraembryonic yolk cell, an important
    player as a signaling\r\nsource and for morphogenesis during gastrulation, as
    a model of ex vivo development.\r\nWe found that dorsal-marginal determinants
    are required and sufficient in these\r\nexplants to form and pattern all three
    germ layers. However, formation of tissues,\r\nwhich require the highest Nodal-signaling
    levels, is variable, demonstrating a\r\ncontribution of extraembryonic tissues
    for reaching peak Nodal signaling levels.\r\nBlastoderm explants also undergo
    gastrulation-like axis elongation. We found that this\r\nelongation movement shows
    hallmarks of oriented mesendoderm cell intercalations\r\ntypically associated
    with dorsal tissues in the intact embryo. These are disrupted by\r\nuniform upregulation
    of BMP signaling activity and concomitant explant ventralization,\r\nsuggesting
    that tight spatial control of BMP signaling is a prerequisite for explant\r\nmorphogenesis.
    This control is achieved by Nodal signaling, which is critical for\r\neffectively
    downregulating BMP signaling in the mesendoderm, highlighting that Nodal\r\nsignaling
    is not only directly required for mesendoderm cell fate specification and\r\nmorphogenesis,
    but also by maintaining low levels of BMP signaling at the dorsal side.\r\nCollectively,
    we provide insights into the capacity and organization of signaling and\r\nmorphogenetic
    domains to recapitulate features of zebrafish gastrulation outside of\r\nthe full
    embryonic context."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
citation:
  ama: 'Schauer A. Mesendoderm formation in zebrafish gastrulation: The role of extraembryonic
    tissues. 2023. doi:<a href="https://doi.org/10.15479/at:ista:12891">10.15479/at:ista:12891</a>'
  apa: 'Schauer, A. (2023). <i>Mesendoderm formation in zebrafish gastrulation: The
    role of extraembryonic tissues</i>. Institute of Science and Technology Austria.
    <a href="https://doi.org/10.15479/at:ista:12891">https://doi.org/10.15479/at:ista:12891</a>'
  chicago: 'Schauer, Alexandra. “Mesendoderm Formation in Zebrafish Gastrulation:
    The Role of Extraembryonic Tissues.” Institute of Science and Technology Austria,
    2023. <a href="https://doi.org/10.15479/at:ista:12891">https://doi.org/10.15479/at:ista:12891</a>.'
  ieee: 'A. Schauer, “Mesendoderm formation in zebrafish gastrulation: The role of
    extraembryonic tissues,” Institute of Science and Technology Austria, 2023.'
  ista: 'Schauer A. 2023. Mesendoderm formation in zebrafish gastrulation: The role
    of extraembryonic tissues. Institute of Science and Technology Austria.'
  mla: 'Schauer, Alexandra. <i>Mesendoderm Formation in Zebrafish Gastrulation: The
    Role of Extraembryonic Tissues</i>. Institute of Science and Technology Austria,
    2023, doi:<a href="https://doi.org/10.15479/at:ista:12891">10.15479/at:ista:12891</a>.'
  short: 'A. Schauer, Mesendoderm Formation in Zebrafish Gastrulation: The Role of
    Extraembryonic Tissues, Institute of Science and Technology Austria, 2023.'
corr_author: '1'
date_created: 2023-05-05T08:48:20Z
date_published: 2023-05-05T00:00:00Z
date_updated: 2025-06-12T06:56:58Z
day: '05'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: CaHe
doi: 10.15479/at:ista:12891
ec_funded: 1
file:
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  date_updated: 2024-05-06T22:30:03Z
  embargo: 2024-05-05
  file_id: '12907'
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  date_updated: 2024-05-06T22:30:03Z
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file_date_updated: 2024-05-06T22:30:03Z
has_accepted_license: '1'
language:
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month: '05'
oa: 1
oa_version: Published Version
page: '190'
project:
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  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
  grant_number: '25239'
  name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
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  - id: '8966'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
title: 'Mesendoderm formation in zebrafish gastrulation: The role of extraembryonic
  tissues'
type: dissertation
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '8966'
abstract:
- lang: eng
  text: During development, a single cell is transformed into a highly complex organism
    through progressive cell division, specification and rearrangement. An important
    prerequisite for the emergence of patterns within the developing organism is to
    establish asymmetries at various scales, ranging from individual cells to the
    entire embryo, eventually giving rise to the different body structures. This becomes
    especially apparent during gastrulation, when the earliest major lineage restriction
    events lead to the formation of the different germ layers. Traditionally, the
    unfolding of the developmental program from symmetry breaking to germ layer formation
    has been studied by dissecting the contributions of different signaling pathways
    and cellular rearrangements in the in vivo context of intact embryos. Recent efforts,
    using the intrinsic capacity of embryonic stem cells to self-assemble and generate
    embryo-like structures de novo, have opened new avenues for understanding the
    many ways by which an embryo can be built and the influence of extrinsic factors
    therein. Here, we discuss and compare divergent and conserved strategies leading
    to germ layer formation in embryos as compared to in vitro systems, their upstream
    molecular cascades and the role of extrinsic factors in this process.
acknowledgement: We thank Nicoletta Petridou, Diana Pinheiro, Cornelia Schwayer and
  Stefania Tavano for feedback on the manuscript. Research in the Heisenberg lab is
  supported by an ERC Advanced Grant (MECSPEC 742573) to C.-P.H. A.S. is a recipient
  of a DOC Fellowship of the Austrian Academy of Science.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Schauer A, Heisenberg C-PJ. Reassembling gastrulation. <i>Developmental Biology</i>.
    2021;474:71-81. doi:<a href="https://doi.org/10.1016/j.ydbio.2020.12.014">10.1016/j.ydbio.2020.12.014</a>
  apa: Schauer, A., &#38; Heisenberg, C.-P. J. (2021). Reassembling gastrulation.
    <i>Developmental Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.ydbio.2020.12.014">https://doi.org/10.1016/j.ydbio.2020.12.014</a>
  chicago: Schauer, Alexandra, and Carl-Philipp J Heisenberg. “Reassembling Gastrulation.”
    <i>Developmental Biology</i>. Elsevier, 2021. <a href="https://doi.org/10.1016/j.ydbio.2020.12.014">https://doi.org/10.1016/j.ydbio.2020.12.014</a>.
  ieee: A. Schauer and C.-P. J. Heisenberg, “Reassembling gastrulation,” <i>Developmental
    Biology</i>, vol. 474. Elsevier, pp. 71–81, 2021.
  ista: Schauer A, Heisenberg C-PJ. 2021. Reassembling gastrulation. Developmental
    Biology. 474, 71–81.
  mla: Schauer, Alexandra, and Carl-Philipp J. Heisenberg. “Reassembling Gastrulation.”
    <i>Developmental Biology</i>, vol. 474, Elsevier, 2021, pp. 71–81, doi:<a href="https://doi.org/10.1016/j.ydbio.2020.12.014">10.1016/j.ydbio.2020.12.014</a>.
  short: A. Schauer, C.-P.J. Heisenberg, Developmental Biology 474 (2021) 71–81.
date_created: 2020-12-22T09:53:34Z
date_published: 2021-06-01T00:00:00Z
date_updated: 2026-06-24T22:30:12Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1016/j.ydbio.2020.12.014
ec_funded: 1
external_id:
  isi:
  - '000639461800008'
  pmid:
  - '33352181'
file:
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  date_updated: 2021-08-11T10:28:06Z
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  file_size: 1440321
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  success: 1
file_date_updated: 2021-08-11T10:28:06Z
has_accepted_license: '1'
intvolume: '       474'
isi: 1
keyword:
- Developmental Biology
- Cell Biology
- Molecular Biology
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 71-81
pmid: 1
project:
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  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
  grant_number: '25239'
  name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
publication: Developmental Biology
publication_identifier:
  issn:
  - 0012-1606
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
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  - id: '12891'
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    status: public
scopus_import: '1'
status: public
title: Reassembling gastrulation
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 474
year: '2021'
...
---
_id: '7888'
abstract:
- lang: eng
  text: Embryonic stem cell cultures are thought to self-organize into embryoid bodies,
    able to undergo symmetry-breaking, germ layer specification and even morphogenesis.
    Yet, it is unclear how to reconcile this remarkable self-organization capacity
    with classical experiments demonstrating key roles for extrinsic biases by maternal
    factors and/or extraembryonic tissues in embryogenesis. Here, we show that zebrafish
    embryonic tissue explants, prepared prior to germ layer induction and lacking
    extraembryonic tissues, can specify all germ layers and form a seemingly complete
    mesendoderm anlage. Importantly, explant organization requires polarized inheritance
    of maternal factors from dorsal-marginal regions of the blastoderm. Moreover,
    induction of endoderm and head-mesoderm, which require peak Nodal-signaling levels,
    is highly variable in explants, reminiscent of embryos with reduced Nodal signals
    from the extraembryonic tissues. Together, these data suggest that zebrafish explants
    do not undergo bona fide self-organization, but rather display features of genetically
    encoded self-assembly, where intrinsic genetic programs control the emergence
    of order.
article_number: e55190
article_processing_charge: No
article_type: original
author:
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: Diana C
  full_name: Nunes Pinheiro, Diana C
  id: 2E839F16-F248-11E8-B48F-1D18A9856A87
  last_name: Nunes Pinheiro
  orcid: 0000-0003-4333-7503
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. Zebrafish embryonic
    explants undergo genetically encoded self-assembly. <i>eLife</i>. 2020;9. doi:<a
    href="https://doi.org/10.7554/elife.55190">10.7554/elife.55190</a>
  apa: Schauer, A., Nunes Pinheiro, D. C., Hauschild, R., &#38; Heisenberg, C.-P.
    J. (2020). Zebrafish embryonic explants undergo genetically encoded self-assembly.
    <i>ELife</i>. eLife Sciences Publications. <a href="https://doi.org/10.7554/elife.55190">https://doi.org/10.7554/elife.55190</a>
  chicago: Schauer, Alexandra, Diana C Nunes Pinheiro, Robert Hauschild, and Carl-Philipp
    J Heisenberg. “Zebrafish Embryonic Explants Undergo Genetically Encoded Self-Assembly.”
    <i>ELife</i>. eLife Sciences Publications, 2020. <a href="https://doi.org/10.7554/elife.55190">https://doi.org/10.7554/elife.55190</a>.
  ieee: A. Schauer, D. C. Nunes Pinheiro, R. Hauschild, and C.-P. J. Heisenberg, “Zebrafish
    embryonic explants undergo genetically encoded self-assembly,” <i>eLife</i>, vol.
    9. eLife Sciences Publications, 2020.
  ista: Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. 2020. Zebrafish
    embryonic explants undergo genetically encoded self-assembly. eLife. 9, e55190.
  mla: Schauer, Alexandra, et al. “Zebrafish Embryonic Explants Undergo Genetically
    Encoded Self-Assembly.” <i>ELife</i>, vol. 9, e55190, eLife Sciences Publications,
    2020, doi:<a href="https://doi.org/10.7554/elife.55190">10.7554/elife.55190</a>.
  short: A. Schauer, D.C. Nunes Pinheiro, R. Hauschild, C.-P.J. Heisenberg, ELife
    9 (2020).
corr_author: '1'
date_created: 2020-05-25T15:01:40Z
date_published: 2020-04-06T00:00:00Z
date_updated: 2026-06-24T22:30:12Z
day: '06'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.7554/elife.55190
ec_funded: 1
external_id:
  isi:
  - '000531544400001'
  pmid:
  - '32250246'
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  content_type: application/pdf
  creator: dernst
  date_created: 2020-05-25T15:15:43Z
  date_updated: 2020-07-14T12:48:04Z
  file_id: '7890'
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has_accepted_license: '1'
intvolume: '         9'
isi: 1
language:
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month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
  grant_number: '25239'
  name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
- _id: 26520D1E-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 850-2017
  name: Coordination of mesendoderm cell fate specification and internalization during
    zebrafish gastrulation
- _id: 266BC5CE-B435-11E9-9278-68D0E5697425
  grant_number: LT000429
  name: Coordination of mesendoderm fate specification and internalization during
    zebrafish gastrulation
publication: eLife
publication_identifier:
  issn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
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  - id: '12891'
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scopus_import: '1'
status: public
title: Zebrafish embryonic explants undergo genetically encoded self-assembly
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7001'
acknowledged_ssus:
- _id: PreCl
- _id: Bio
article_processing_charge: No
article_type: original
author:
- first_name: Cornelia
  full_name: Schwayer, Cornelia
  id: 3436488C-F248-11E8-B48F-1D18A9856A87
  last_name: Schwayer
  orcid: 0000-0001-5130-2226
- first_name: Shayan
  full_name: Shamipour, Shayan
  id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
  last_name: Shamipour
- first_name: Kornelija
  full_name: Pranjic-Ferscha, Kornelija
  id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
  last_name: Pranjic-Ferscha
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: M
  full_name: Balda, M
  last_name: Balda
- first_name: M
  full_name: Tada, M
  last_name: Tada
- first_name: K
  full_name: Matter, K
  last_name: Matter
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Schwayer C, Shamipour S, Pranjic-Ferscha K, et al. Mechanosensation of tight
    junctions depends on ZO-1 phase separation and flow. <i>Cell</i>. 2019;179(4):937-952.e18.
    doi:<a href="https://doi.org/10.1016/j.cell.2019.10.006">10.1016/j.cell.2019.10.006</a>
  apa: Schwayer, C., Shamipour, S., Pranjic-Ferscha, K., Schauer, A., Balda, M., Tada,
    M., … Heisenberg, C.-P. J. (2019). Mechanosensation of tight junctions depends
    on ZO-1 phase separation and flow. <i>Cell</i>. Cell Press. <a href="https://doi.org/10.1016/j.cell.2019.10.006">https://doi.org/10.1016/j.cell.2019.10.006</a>
  chicago: Schwayer, Cornelia, Shayan Shamipour, Kornelija Pranjic-Ferscha, Alexandra
    Schauer, M Balda, M Tada, K Matter, and Carl-Philipp J Heisenberg. “Mechanosensation
    of Tight Junctions Depends on ZO-1 Phase Separation and Flow.” <i>Cell</i>. Cell
    Press, 2019. <a href="https://doi.org/10.1016/j.cell.2019.10.006">https://doi.org/10.1016/j.cell.2019.10.006</a>.
  ieee: C. Schwayer <i>et al.</i>, “Mechanosensation of tight junctions depends on
    ZO-1 phase separation and flow,” <i>Cell</i>, vol. 179, no. 4. Cell Press, p.
    937–952.e18, 2019.
  ista: Schwayer C, Shamipour S, Pranjic-Ferscha K, Schauer A, Balda M, Tada M, Matter
    K, Heisenberg C-PJ. 2019. Mechanosensation of tight junctions depends on ZO-1
    phase separation and flow. Cell. 179(4), 937–952.e18.
  mla: Schwayer, Cornelia, et al. “Mechanosensation of Tight Junctions Depends on
    ZO-1 Phase Separation and Flow.” <i>Cell</i>, vol. 179, no. 4, Cell Press, 2019,
    p. 937–952.e18, doi:<a href="https://doi.org/10.1016/j.cell.2019.10.006">10.1016/j.cell.2019.10.006</a>.
  short: C. Schwayer, S. Shamipour, K. Pranjic-Ferscha, A. Schauer, M. Balda, M. Tada,
    K. Matter, C.-P.J. Heisenberg, Cell 179 (2019) 937–952.e18.
date_created: 2019-11-12T12:51:06Z
date_published: 2019-10-31T00:00:00Z
date_updated: 2026-06-24T22:31:00Z
day: '31'
ddc:
- '570'
department:
- _id: CaHe
- _id: BjHo
doi: 10.1016/j.cell.2019.10.006
ec_funded: 1
external_id:
  isi:
  - '000493898000012'
  pmid:
  - '31675500'
file:
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intvolume: '       179'
isi: 1
issue: '4'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 937-952.e18
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
publication: Cell
publication_identifier:
  eissn:
  - 1097-4172
  issn:
  - 0092-8674
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
  link:
  - description: News auf IST Website
    relation: press_release
    url: https://ist.ac.at/en/news/biochemistry-meets-mechanics-the-sensitive-nature-of-cell-cell-contact-formation-in-embryo-development/
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  - id: '8350'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Mechanosensation of tight junctions depends on ZO-1 phase separation and flow
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 179
year: '2019'
...