[{"quality_controlled":"1","type":"journal_article","external_id":{"isi":["000570187900001"],"pmid":["32810889"]},"language":[{"iso":"eng"}],"_id":"8582","abstract":[{"lang":"eng","text":"Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear.\r\nHere, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze‐fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall components as well as connections between the cell wall and the plasma membrane.\r\nThis study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems."}],"citation":{"short":"H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto, K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke, X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.","ieee":"H. Li <i>et al.</i>, “Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 1. Wiley, pp. 351–369, 2021.","mla":"Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 1, Wiley, 2021, pp. 351–69, doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>.","apa":"Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto, S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>","ista":"Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytologist. 229(1), 351–369.","ama":"Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(1):351-369. doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>","chicago":"Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>."},"day":"01","has_accepted_license":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["580"],"year":"2021","intvolume":"       229","date_published":"2021-01-01T00:00:00Z","file_date_updated":"2021-02-04T09:44:17Z","isi":1,"acknowledged_ssus":[{"_id":"Bio"}],"date_updated":"2025-06-12T06:32:24Z","publication":"New Phytologist","page":"351-369","status":"public","scopus_import":"1","issue":"1","ec_funded":1,"publication_status":"published","file":[{"access_level":"open_access","success":1,"content_type":"application/pdf","file_size":4061962,"date_created":"2021-02-04T09:44:17Z","checksum":"b45621607b4cab97eeb1605ab58e896e","file_name":"2021_NewPhytologist_Li.pdf","date_updated":"2021-02-04T09:44:17Z","file_id":"9084","creator":"dernst","relation":"main_file"}],"publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"oa_version":"Published Version","date_created":"2020-09-28T08:59:28Z","title":"Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana","oa":1,"doi":"10.1111/nph.16887","acknowledgement":"We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg) for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1 mutant and the complementation lines, and Dr Patricia C. Zambryski (University of California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria) for assistance with imaging, group members for discussions, Martine De Cock for help in preparing the manuscript and Nataliia Gnyliukh for critical reading and revision of the manuscript. This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 742985) and Comisión Nacional de Investigación Científica y Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007‐2013) under REA grant agreement no. 291734.","department":[{"_id":"JiFr"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"EvBe"}],"month":"01","article_type":"original","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"}],"author":[{"full_name":"Li, Hongjiang","last_name":"Li","orcid":"0000-0001-5039-9660","first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87"},{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","orcid":"0000-0002-6862-1247","last_name":"von Wangenheim","full_name":"von Wangenheim, Daniel"},{"orcid":"0000-0001-7048-4627","first_name":"Xixi","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","full_name":"Zhang, Xixi","last_name":"Zhang"},{"last_name":"Tan","full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","first_name":"Shutang","orcid":"0000-0002-0471-8285"},{"orcid":"0000-0002-8821-8236","id":"39CD9926-F248-11E8-B48F-1D18A9856A87","first_name":"Nasser","full_name":"Darwish-Miranda, Nasser","last_name":"Darwish-Miranda"},{"last_name":"Naramoto","full_name":"Naramoto, Satoshi","first_name":"Satoshi"},{"id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","first_name":"Krzysztof T","orcid":"0000-0001-7263-0560","last_name":"Wabnik","full_name":"Wabnik, Krzysztof T"},{"first_name":"Riet","last_name":"de Rycke","full_name":"de Rycke, Riet"},{"orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","last_name":"Kaufmann","full_name":"Kaufmann, Walter"},{"full_name":"Gütl, Daniel J","last_name":"Gütl","id":"381929CE-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel J"},{"first_name":"Ricardo","last_name":"Tejos","full_name":"Tejos, Ricardo"},{"first_name":"Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","full_name":"Grones, Peter"},{"first_name":"Meiyu","full_name":"Ke, Meiyu","last_name":"Ke"},{"first_name":"Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","last_name":"Chen","full_name":"Chen, Xu"},{"full_name":"Dettmer, Jan","last_name":"Dettmer","first_name":"Jan"},{"last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"volume":229,"publisher":"Wiley","article_processing_charge":"Yes (via OA deal)","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"pmid":1},{"day":"19","citation":{"apa":"Łangowski, Ł., Wabnik, K. T., Li, H., Vanneste, S., Naramoto, S., Tanaka, H., &#38; Friml, J. (2016). Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. <i>Cell Discovery</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/celldisc.2016.18\">https://doi.org/10.1038/celldisc.2016.18</a>","mla":"Łangowski, Łukasz, et al. “Cellular Mechanisms for Cargo Delivery and Polarity Maintenance at Different Polar Domains in Plant Cells.” <i>Cell Discovery</i>, vol. 2, 16018, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/celldisc.2016.18\">10.1038/celldisc.2016.18</a>.","short":"Ł. Łangowski, K.T. Wabnik, H. Li, S. Vanneste, S. Naramoto, H. Tanaka, J. Friml, Cell Discovery 2 (2016).","ieee":"Ł. Łangowski <i>et al.</i>, “Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells,” <i>Cell Discovery</i>, vol. 2. Nature Publishing Group, 2016.","chicago":"Łangowski, Łukasz, Krzysztof T Wabnik, Hongjiang Li, Steffen Vanneste, Satoshi Naramoto, Hirokazu Tanaka, and Jiří Friml. “Cellular Mechanisms for Cargo Delivery and Polarity Maintenance at Different Polar Domains in Plant Cells.” <i>Cell Discovery</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/celldisc.2016.18\">https://doi.org/10.1038/celldisc.2016.18</a>.","ama":"Łangowski Ł, Wabnik KT, Li H, et al. Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. <i>Cell Discovery</i>. 2016;2. doi:<a href=\"https://doi.org/10.1038/celldisc.2016.18\">10.1038/celldisc.2016.18</a>","ista":"Łangowski Ł, Wabnik KT, Li H, Vanneste S, Naramoto S, Tanaka H, Friml J. 2016. Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. Cell Discovery. 2, 16018."},"has_accepted_license":"1","quality_controlled":"1","type":"journal_article","external_id":{"isi":["000414797400001"]},"language":[{"iso":"eng"}],"_id":"1081","abstract":[{"text":"The asymmetric localization of proteins in the plasma membrane domains of eukaryotic cells is a fundamental manifestation of cell polarity that is central to multicellular organization and developmental patterning. In plants, the mechanisms underlying the polar localization of cargo proteins are still largely unknown and appear to be fundamentally distinct from those operating in mammals. Here, we present a systematic, quantitative comparative analysis of the polar delivery and subcellular localization of proteins that characterize distinct polar plasma membrane domains in plant cells. The combination of microscopic analyses and computational modeling revealed a mechanistic framework common to diverse polar cargos and underlying the establishment and maintenance of apical, basal, and lateral polar domains in plant cells. This mechanism depends on the polar secretion, constitutive endocytic recycling, and restricted lateral diffusion of cargos within the plasma membrane. Moreover, our observations suggest that polar cargo distribution involves the individual protein potential to form clusters within the plasma membrane and interact with the extracellular matrix. Our observations provide insights into the shared cellular mechanisms of polar cargo delivery and polarity maintenance in plant cells.","lang":"eng"}],"date_published":"2016-07-19T00:00:00Z","ddc":["580"],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2016","intvolume":"         2","article_number":"16018","isi":1,"date_updated":"2025-09-22T14:19:46Z","publication":"Cell Discovery","file_date_updated":"2018-12-12T10:13:33Z","scopus_import":"1","ec_funded":1,"status":"public","file":[{"file_name":"IST-2017-757-v1+1_celldisc201618.pdf","creator":"system","relation":"main_file","date_updated":"2018-12-12T10:13:33Z","file_id":"5017","access_level":"open_access","content_type":"application/pdf","date_created":"2018-12-12T10:13:33Z","file_size":5261671}],"publication_status":"published","publist_id":"6299","oa_version":"Published Version","date_created":"2018-12-11T11:50:02Z","title":"Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells","month":"07","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300","name":"Polarity and subcellular dynamics in plants"}],"volume":2,"author":[{"first_name":"Łukasz","last_name":"Łangowski","full_name":"Łangowski, Łukasz"},{"orcid":"0000-0001-7263-0560","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","first_name":"Krzysztof T","full_name":"Wabnik, Krzysztof T","last_name":"Wabnik"},{"last_name":"Li","full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","first_name":"Hongjiang","orcid":"0000-0001-5039-9660"},{"full_name":"Vanneste, Steffen","last_name":"Vanneste","first_name":"Steffen"},{"last_name":"Naramoto","full_name":"Naramoto, Satoshi","first_name":"Satoshi"},{"first_name":"Hirokazu","last_name":"Tanaka","full_name":"Tanaka, Hirokazu"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí"}],"pubrep_id":"757","doi":"10.1038/celldisc.2016.18","oa":1,"acknowledgement":"We thank Bonnie Bartel, Jenny Russinova and Niko Geldner\r\nfor sharing published material, Martine de Cock and Annick\r\nBleys for help in preparing the manuscript. This work was\r\nsupported by the European Research Council (project\r\nERC-2011-StG-20101109-PSDP); Czech Science Foundation\r\nGAČR (GA13-40637S); project CEITEC—Central European\r\nInstitute of Technology (CZ.1.05/1.1.00/02.0068). SV is a\r\npostdoctoral fellow of the Research Foundation-Flanders.\r\nSN is a Project Assistant Professor supported by the Japanese\r\nSociety for the Promotion of Science (JSPS; 30612022 to SN),\r\nthe NC-CARP project of the Ministry of Education, Culture,\r\nSports, Science and Technology in Japan to SN.","publisher":"Nature Publishing Group","article_processing_charge":"No","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"date_published":"2015-08-01T00:00:00Z","year":"2015","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","ddc":["570"],"intvolume":"        66","has_accepted_license":"1","day":"01","citation":{"chicago":"Jia, Yuebin, Huiyu Tian, Hongjiang Li, Qianqian Yu, Lei Wang, Jiří Friml, and Zhaojun Ding. “The Arabidopsis Thaliana Elongator Complex Subunit 2 Epigenetically Affects Root Development.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2015. <a href=\"https://doi.org/10.1093/jxb/erv230\">https://doi.org/10.1093/jxb/erv230</a>.","ama":"Jia Y, Tian H, Li H, et al. The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development. <i>Journal of Experimental Botany</i>. 2015;66(15):4631-4642. doi:<a href=\"https://doi.org/10.1093/jxb/erv230\">10.1093/jxb/erv230</a>","ista":"Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z. 2015. The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development. Journal of Experimental Botany. 66(15), 4631–4642.","apa":"Jia, Y., Tian, H., Li, H., Yu, Q., Wang, L., Friml, J., &#38; Ding, Z. (2015). The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/erv230\">https://doi.org/10.1093/jxb/erv230</a>","mla":"Jia, Yuebin, et al. “The Arabidopsis Thaliana Elongator Complex Subunit 2 Epigenetically Affects Root Development.” <i>Journal of Experimental Botany</i>, vol. 66, no. 15, Oxford University Press, 2015, pp. 4631–42, doi:<a href=\"https://doi.org/10.1093/jxb/erv230\">10.1093/jxb/erv230</a>.","short":"Y. Jia, H. Tian, H. Li, Q. Yu, L. Wang, J. Friml, Z. Ding, Journal of Experimental Botany 66 (2015) 4631–4642.","ieee":"Y. Jia <i>et al.</i>, “The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development,” <i>Journal of Experimental Botany</i>, vol. 66, no. 15. Oxford University Press, pp. 4631–4642, 2015."},"type":"journal_article","quality_controlled":"1","abstract":[{"text":"The elongator complex subunit 2 (ELP2) protein, one subunit of an evolutionarily conserved histone acetyltransferase complex, has been shown to participate in leaf patterning, plant immune and abiotic stress responses in Arabidopsis thaliana. Here, its role in root development was explored. Compared to the wild type, the elp2 mutant exhibited an accelerated differentiation of its root stem cells and cell division was more active in its quiescent centre (QC). The key transcription factors responsible for maintaining root stem cell and QC identity, such as AP2 transcription factors PLT1 (PLETHORA1) and PLT2 (PLETHORA2), GRAS transcription factors such as SCR (SCARECROW) and SHR (SHORT ROOT) and WUSCHEL-RELATED HOMEOBOX5 transcription factor WOX5, were all strongly down-regulated in the mutant. On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2. The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip. Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.","lang":"eng"}],"_id":"1556","language":[{"iso":"eng"}],"external_id":{"isi":["000359687400017"]},"scopus_import":"1","issue":"15","status":"public","page":"4631 - 4642","isi":1,"publication":"Journal of Experimental Botany","date_updated":"2025-09-23T13:42:27Z","file_date_updated":"2020-07-14T12:45:02Z","oa_version":"Published Version","date_created":"2018-12-11T11:52:42Z","title":"The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development","file":[{"access_level":"open_access","content_type":"application/pdf","file_size":7753043,"date_created":"2018-12-12T10:14:02Z","checksum":"257919be0ce3d306185d3891ad7acf39","file_name":"IST-2016-480-v1+1_J._Exp._Bot.-2015-Jia-4631-42.pdf","relation":"main_file","file_id":"5051","creator":"system","date_updated":"2020-07-14T12:45:02Z"}],"publist_id":"5615","publication_status":"published","publisher":"Oxford University Press","tmp":{"short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"article_processing_charge":"No","department":[{"_id":"JiFr"}],"month":"08","author":[{"first_name":"Yuebin","full_name":"Jia, Yuebin","last_name":"Jia"},{"first_name":"Huiyu","full_name":"Tian, Huiyu","last_name":"Tian"},{"last_name":"Li","full_name":"Li, Hongjiang","orcid":"0000-0001-5039-9660","first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Qianqian","last_name":"Yu","full_name":"Yu, Qianqian"},{"full_name":"Wang, Lei","last_name":"Wang","first_name":"Lei"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml"},{"last_name":"Ding","full_name":"Ding, Zhaojun","first_name":"Zhaojun"}],"volume":66,"pubrep_id":"480","doi":"10.1093/jxb/erv230","oa":1},{"status":"public","page":"670 - 683","scopus_import":"1","issue":"3","isi":1,"date_updated":"2025-09-23T08:40:43Z","publication":"Cell","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2015","intvolume":"       163","date_published":"2015-10-22T00:00:00Z","quality_controlled":"1","type":"journal_article","external_id":{"isi":["000364828900020"]},"language":[{"iso":"eng"}],"_id":"532","abstract":[{"lang":"eng","text":"Ethylene is a gaseous phytohormone that plays vital roles in plant growth and development. Previous studies uncovered EIN2 as an essential signal transducer linking ethylene perception on ER to transcriptional regulation in the nucleus through a “cleave and shuttle” model. In this study, we report another mechanism of EIN2-mediated ethylene signaling, whereby EIN2 imposes the translational repression of EBF1 and EBF2 mRNA. We find that the EBF1/2 3′ UTRs mediate EIN2-directed translational repression and identify multiple poly-uridylates (PolyU) motifs as functional cis elements of 3′ UTRs. Furthermore, we demonstrate that ethylene induces EIN2 to associate with 3′ UTRs and target EBF1/2 mRNA to cytoplasmic processing-body (P-body) through interacting with multiple P-body factors, including EIN5 and PABs. Our study illustrates translational regulation as a key step in ethylene signaling and presents mRNA 3′ UTR functioning as a “signal transducer” to sense and relay cellular signaling in plants."}],"citation":{"mla":"Li, Wenyang, et al. “EIN2-Directed Translational Regulation of Ethylene Signaling in Arabidopsis.” <i>Cell</i>, vol. 163, no. 3, Cell Press, 2015, pp. 670–83, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.09.037\">10.1016/j.cell.2015.09.037</a>.","ieee":"W. Li <i>et al.</i>, “EIN2-directed translational regulation of ethylene signaling in arabidopsis,” <i>Cell</i>, vol. 163, no. 3. Cell Press, pp. 670–683, 2015.","short":"W. Li, M. Ma, Y. Feng, H. Li, Y. Wang, Y. Ma, M. Li, F. An, H. Guo, Cell 163 (2015) 670–683.","apa":"Li, W., Ma, M., Feng, Y., Li, H., Wang, Y., Ma, Y., … Guo, H. (2015). EIN2-directed translational regulation of ethylene signaling in arabidopsis. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.09.037\">https://doi.org/10.1016/j.cell.2015.09.037</a>","ama":"Li W, Ma M, Feng Y, et al. EIN2-directed translational regulation of ethylene signaling in arabidopsis. <i>Cell</i>. 2015;163(3):670-683. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.09.037\">10.1016/j.cell.2015.09.037</a>","ista":"Li W, Ma M, Feng Y, Li H, Wang Y, Ma Y, Li M, An F, Guo H. 2015. EIN2-directed translational regulation of ethylene signaling in arabidopsis. Cell. 163(3), 670–683.","chicago":"Li, Wenyang, Mengdi Ma, Ying Feng, Hongjiang Li, Yichuan Wang, Yutong Ma, Mingzhe Li, Fengying An, and Hongwei Guo. “EIN2-Directed Translational Regulation of Ethylene Signaling in Arabidopsis.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.09.037\">https://doi.org/10.1016/j.cell.2015.09.037</a>."},"day":"22","publisher":"Cell Press","article_processing_charge":"No","doi":"10.1016/j.cell.2015.09.037","department":[{"_id":"JiFr"}],"month":"10","author":[{"full_name":"Li, Wenyang","last_name":"Li","first_name":"Wenyang"},{"last_name":"Ma","full_name":"Ma, Mengdi","first_name":"Mengdi"},{"first_name":"Ying","full_name":"Feng, Ying","last_name":"Feng"},{"orcid":"0000-0001-5039-9660","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","first_name":"Hongjiang","full_name":"Li, Hongjiang","last_name":"Li"},{"full_name":"Wang, Yichuan","last_name":"Wang","first_name":"Yichuan"},{"full_name":"Ma, Yutong","last_name":"Ma","first_name":"Yutong"},{"first_name":"Mingzhe","full_name":"Li, Mingzhe","last_name":"Li"},{"last_name":"An","full_name":"An, Fengying","first_name":"Fengying"},{"first_name":"Hongwei","last_name":"Guo","full_name":"Guo, Hongwei"}],"volume":163,"oa_version":"None","title":"EIN2-directed translational regulation of ethylene signaling in arabidopsis","date_created":"2018-12-11T11:47:00Z","publication_status":"published","publist_id":"7285"},{"abstract":[{"lang":"eng","text":"The prominent and evolutionarily ancient role of the plant hormone auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating cells exogenous application of auxin or redistribution of endogenous auxin induces very rapid microtubule re-orientation from transverse to longitudinal, coherent with the inhibition of cell expansion. This fast auxin effect requires auxin binding protein 1 (ABP1) and involves a contribution of downstream signalling components such as ROP6 GTPase, ROP-interactive protein RIC1 and the microtubule-severing protein katanin. These components are required for rapid auxin-and ABP1-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses."}],"_id":"1862","language":[{"iso":"eng"}],"external_id":{"pmid":["25409144"],"isi":["000346310800045"]},"type":"journal_article","quality_controlled":"1","day":"04","citation":{"chicago":"Chen, Xu, Laurie Grandont, Hongjiang Li, Robert Hauschild, Sébastien Paque, Anas Abuzeineh, Hana Rakusova, Eva Benková, Catherine Perrot Rechenmann, and Jiří Friml. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” <i>Nature</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/nature13889\">https://doi.org/10.1038/nature13889</a>.","ama":"Chen X, Grandont L, Li H, et al. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. <i>Nature</i>. 2014;516(729):90-93. doi:<a href=\"https://doi.org/10.1038/nature13889\">10.1038/nature13889</a>","ista":"Chen X, Grandont L, Li H, Hauschild R, Paque S, Abuzeineh A, Rakusova H, Benková E, Perrot Rechenmann C, Friml J. 2014. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. Nature. 516(729), 90–93.","apa":"Chen, X., Grandont, L., Li, H., Hauschild, R., Paque, S., Abuzeineh, A., … Friml, J. (2014). Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature13889\">https://doi.org/10.1038/nature13889</a>","mla":"Chen, Xu, et al. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” <i>Nature</i>, vol. 516, no. 729, Nature Publishing Group, 2014, pp. 90–93, doi:<a href=\"https://doi.org/10.1038/nature13889\">10.1038/nature13889</a>.","ieee":"X. Chen <i>et al.</i>, “Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules,” <i>Nature</i>, vol. 516, no. 729. Nature Publishing Group, pp. 90–93, 2014.","short":"X. Chen, L. Grandont, H. Li, R. Hauschild, S. Paque, A. Abuzeineh, H. Rakusova, E. Benková, C. Perrot Rechenmann, J. Friml, Nature 516 (2014) 90–93."},"intvolume":"       516","year":"2014","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2014-12-04T00:00:00Z","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4257754/","open_access":"1"}],"date_updated":"2025-09-29T13:10:05Z","publication":"Nature","isi":1,"page":"90 - 93","status":"public","issue":"729","ec_funded":1,"scopus_import":"1","publist_id":"5237","publication_status":"published","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"title":"Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules","date_created":"2018-12-11T11:54:25Z","oa_version":"Submitted Version","acknowledgement":"We thank R. Dixit for performing complementary experiments, D. W. Ehrhardt and T. Hashimoto for providing the seeds of TUB6–RFP and EB1b–GFP respectively, E. Zazimalova, J. Petrasek and M. Fendrych for discussing the manuscript and J. Leung for text optimization. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, to J.F.), ANR blanc AuxiWall project (ANR-11-BSV5-0007, to C.P.-R. and L.G.) and the Agency for Innovation by Science and Technology (IWT) (to H.R.). This work benefited from the facilities and expertise of the Imagif Cell Biology platform (http://www.imagif.cnrs.fr), which is supported by the Conseil Général de l’Essonne.","oa":1,"doi":"10.1038/nature13889","volume":516,"author":[{"full_name":"Chen, Xu","last_name":"Chen","first_name":"Xu","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Laurie","full_name":"Grandont, Laurie","last_name":"Grandont"},{"last_name":"Li","full_name":"Li, Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","first_name":"Hongjiang","orcid":"0000-0001-5039-9660"},{"full_name":"Hauschild, Robert","last_name":"Hauschild","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sébastien","last_name":"Paque","full_name":"Paque, Sébastien"},{"first_name":"Anas","last_name":"Abuzeineh","full_name":"Abuzeineh, Anas"},{"id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA","first_name":"Hana","last_name":"Rakusova","full_name":"Rakusova, Hana"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"first_name":"Catherine","full_name":"Perrot Rechenmann, Catherine","last_name":"Perrot Rechenmann"},{"last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"article_type":"original","project":[{"name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300"}],"department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"EvBe"}],"month":"12","corr_author":"1","article_processing_charge":"No","publisher":"Nature Publishing Group","pmid":1},{"external_id":{"isi":["000332309600046"],"pmid":["24578577"]},"language":[{"iso":"eng"}],"_id":"1917","abstract":[{"text":"Auxin-binding protein 1 (ABP1) was discovered nearly 40 years ago and was shown to be essential for plant development and morphogenesis, but its mode of action remains unclear. Here, we report that the plasma membrane-localized transmembrane kinase (TMK) receptor-like kinases interact with ABP1 and transduce auxin signal to activate plasma membrane-associated ROPs [Rho-like guanosine triphosphatases (GTPase) from plants], leading to changes in the cytoskeleton and the shape of leaf pavement cells in Arabidopsis. The interaction between ABP1 and TMK at the cell surface is induced by auxin and requires ABP1 sensing of auxin. These findings show that TMK proteins and ABP1 form a cell surface auxin perception complex that activates ROP signaling pathways, regulating nontranscriptional cytoplasmic responses and associated fundamental processes.","lang":"eng"}],"quality_controlled":"1","type":"journal_article","day":"28","citation":{"mla":"Xu, Tongda, et al. “Cell Surface ABP1-TMK Auxin Sensing Complex Activates ROP GTPase Signaling.” <i>Science</i>, vol. 343, no. 6174, American Association for the Advancement of Science, 2014, pp. 1025–28, doi:<a href=\"https://doi.org/10.1126/science.1245125\">10.1126/science.1245125</a>.","ieee":"T. Xu <i>et al.</i>, “Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling,” <i>Science</i>, vol. 343, no. 6174. American Association for the Advancement of Science, pp. 1025–1028, 2014.","short":"T. Xu, N. Dai, J. Chen, S. Nagawa, M. Cao, H. Li, Z. Zhou, X. Chen, R. De Rycke, H. Rakusová, W. Wang, A. Jones, J. Friml, S. Patterson, A. Bleecker, Z. Yang, Science 343 (2014) 1025–1028.","apa":"Xu, T., Dai, N., Chen, J., Nagawa, S., Cao, M., Li, H., … Yang, Z. (2014). Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1245125\">https://doi.org/10.1126/science.1245125</a>","ama":"Xu T, Dai N, Chen J, et al. Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. <i>Science</i>. 2014;343(6174):1025-1028. doi:<a href=\"https://doi.org/10.1126/science.1245125\">10.1126/science.1245125</a>","ista":"Xu T, Dai N, Chen J, Nagawa S, Cao M, Li H, Zhou Z, Chen X, De Rycke R, Rakusová H, Wang W, Jones A, Friml J, Patterson S, Bleecker A, Yang Z. 2014. Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling. Science. 343(6174), 1025–1028.","chicago":"Xu, Tongda, Ning Dai, Jisheng Chen, Shingo Nagawa, Min Cao, Hongjiang Li, Zimin Zhou, et al. “Cell Surface ABP1-TMK Auxin Sensing Complex Activates ROP GTPase Signaling.” <i>Science</i>. American Association for the Advancement of Science, 2014. <a href=\"https://doi.org/10.1126/science.1245125\">https://doi.org/10.1126/science.1245125</a>."},"intvolume":"       343","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2014","date_published":"2014-02-28T00:00:00Z","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166562/","open_access":"1"}],"date_updated":"2025-09-29T12:20:10Z","publication":"Science","isi":1,"page":"1025 - 1028","status":"public","issue":"6174","scopus_import":"1","publication_status":"published","publist_id":"5177","date_created":"2018-12-11T11:54:42Z","title":"Cell surface ABP1-TMK auxin sensing complex activates ROP GTPase signaling","oa_version":"Submitted Version","doi":"10.1126/science.1245125","oa":1,"acknowledgement":"Supported by the intramural research program of the National Institute of Arthritis and Musculoskeletal and Skin Diseases and by its Laboratory Animal Care and Use Section and Flow Cytometry Group, Office of Science and Technology","author":[{"first_name":"Tongda","last_name":"Xu","full_name":"Xu, Tongda"},{"first_name":"Ning","full_name":"Dai, Ning","last_name":"Dai"},{"first_name":"Jisheng","last_name":"Chen","full_name":"Chen, Jisheng"},{"first_name":"Shingo","full_name":"Nagawa, Shingo","last_name":"Nagawa"},{"full_name":"Cao, Min","last_name":"Cao","first_name":"Min"},{"first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660","last_name":"Li","full_name":"Li, Hongjiang"},{"first_name":"Zimin","full_name":"Zhou, Zimin","last_name":"Zhou"},{"id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","first_name":"Xu","full_name":"Chen, Xu","last_name":"Chen"},{"first_name":"Riet","last_name":"De Rycke","full_name":"De Rycke, Riet"},{"first_name":"Hana","full_name":"Rakusová, Hana","last_name":"Rakusová"},{"last_name":"Wang","full_name":"Wang, Wen","first_name":"Wen"},{"last_name":"Jones","full_name":"Jones, Alan","first_name":"Alan"},{"full_name":"Friml, Jirí","last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Patterson","full_name":"Patterson, Sara","first_name":"Sara"},{"first_name":"Anthony","full_name":"Bleecker, Anthony","last_name":"Bleecker"},{"last_name":"Yang","full_name":"Yang, Zhenbiao","first_name":"Zhenbiao"}],"volume":343,"department":[{"_id":"JiFr"}],"month":"02","article_type":"original","article_processing_charge":"No","publisher":"American Association for the Advancement of Science","pmid":1},{"publication":"Plant Cell","date_updated":"2025-09-29T12:17:33Z","isi":1,"status":"public","page":"2114 - 2128","ec_funded":1,"issue":"5","scopus_import":"1","external_id":{"isi":["000338771700027"]},"abstract":[{"lang":"eng","text":"Cell polarity manifested by asymmetric distribution of cargoes, such as receptors and transporters, within the plasma membrane (PM) is crucial for essential functions in multicellular organisms. In plants, cell polarity (re)establishment is intimately linked to patterning processes. Despite the importance of cell polarity, its underlying mechanisms are still largely unknown, including the definition and distinctiveness of the polar domains within the PM. Here, we show in Arabidopsis thaliana that the signaling membrane components, the phosphoinositides phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4, 5-bisphosphate [PtdIns(4, 5)P2] as well as PtdIns4P 5-kinases mediating their interconversion, are specifically enriched at apical and basal polar plasma membrane domains. The PtdIns4P 5-kinases PIP5K1 and PIP5K2 are redundantly required for polar localization of specifically apical and basal cargoes, such as PIN-FORMED transporters for the plant hormone auxin. As a consequence of the polarity defects, instructive auxin gradients as well as embryonic and postembryonic patterning are severely compromised. Furthermore, auxin itself regulates PIP5K transcription and PtdIns4P and PtdIns(4, 5)P2 levels, in particular their association with polar PM domains. Our results provide insight into the polar domain-delineating mechanisms in plant cells that depend on apical and basal distribution of membrane lipids and are essential for embryonic and postembryonic patterning."}],"_id":"1921","language":[{"iso":"eng"}],"type":"journal_article","day":"01","citation":{"mla":"Tejos, Ricardo, et al. “Bipolar Plasma Membrane Distribution of Phosphoinositides and Their Requirement for Auxin-Mediated Cell Polarity and Patterning in Arabidopsis.” <i>Plant Cell</i>, vol. 26, no. 5, American Society of Plant Biologists, 2014, pp. 2114–28, doi:<a href=\"https://doi.org/10.1105/tpc.114.126185\">10.1105/tpc.114.126185</a>.","short":"R. Tejos, M. Sauer, S. Vanneste, M. Palacios-Gomez, H. Li, M. Heilmann, R. Van Wijk, J. Vermeer, I. Heilmann, T. Munnik, J. Friml, Plant Cell 26 (2014) 2114–2128.","ieee":"R. Tejos <i>et al.</i>, “Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis,” <i>Plant Cell</i>, vol. 26, no. 5. American Society of Plant Biologists, pp. 2114–2128, 2014.","apa":"Tejos, R., Sauer, M., Vanneste, S., Palacios-Gomez, M., Li, H., Heilmann, M., … Friml, J. (2014). Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.114.126185\">https://doi.org/10.1105/tpc.114.126185</a>","ama":"Tejos R, Sauer M, Vanneste S, et al. Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. <i>Plant Cell</i>. 2014;26(5):2114-2128. doi:<a href=\"https://doi.org/10.1105/tpc.114.126185\">10.1105/tpc.114.126185</a>","ista":"Tejos R, Sauer M, Vanneste S, Palacios-Gomez M, Li H, Heilmann M, Van Wijk R, Vermeer J, Heilmann I, Munnik T, Friml J. 2014. Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis. Plant Cell. 26(5), 2114–2128.","chicago":"Tejos, Ricardo, Michael Sauer, Steffen Vanneste, MiriamPalacios  Palacios-Gomez, Hongjiang Li, Mareike Heilmann, Ringo Van Wijk, et al. “Bipolar Plasma Membrane Distribution of Phosphoinositides and Their Requirement for Auxin-Mediated Cell Polarity and Patterning in Arabidopsis.” <i>Plant Cell</i>. American Society of Plant Biologists, 2014. <a href=\"https://doi.org/10.1105/tpc.114.126185\">https://doi.org/10.1105/tpc.114.126185</a>."},"intvolume":"        26","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2014","date_published":"2014-05-01T00:00:00Z","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079372/","open_access":"1"}],"doi":"10.1105/tpc.114.126185","oa":1,"acknowledgement":"This work was supported by grants from the Odysseus program of the Research Foundation-Flanders (to J.F.).","volume":26,"author":[{"first_name":"Ricardo","full_name":"Tejos, Ricardo","last_name":"Tejos"},{"first_name":"Michael","last_name":"Sauer","full_name":"Sauer, Michael"},{"first_name":"Steffen","full_name":"Vanneste, Steffen","last_name":"Vanneste"},{"last_name":"Palacios-Gomez","full_name":"Palacios-Gomez, MiriamPalacios ","first_name":"MiriamPalacios "},{"full_name":"Li, Hongjiang","last_name":"Li","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","first_name":"Hongjiang","orcid":"0000-0001-5039-9660"},{"first_name":"Mareike","last_name":"Heilmann","full_name":"Heilmann, Mareike"},{"full_name":"Van Wijk, Ringo","last_name":"Van Wijk","first_name":"Ringo"},{"first_name":"Joop","full_name":"Vermeer, Joop","last_name":"Vermeer"},{"first_name":"Ingo","full_name":"Heilmann, Ingo","last_name":"Heilmann"},{"last_name":"Munnik","full_name":"Munnik, Teun","first_name":"Teun"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí"}],"month":"05","department":[{"_id":"JiFr"}],"project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300","name":"Polarity and subcellular dynamics in plants"}],"article_processing_charge":"No","corr_author":"1","publisher":"American Society of Plant Biologists","publication_status":"published","publist_id":"5173","title":"Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis","date_created":"2018-12-11T11:54:43Z","oa_version":"Submitted Version"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","year":"2013","intvolume":"       110","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651428/","open_access":"1"}],"date_published":"2013-05-07T00:00:00Z","quality_controlled":"1","type":"journal_article","external_id":{"pmid":["23613581"],"isi":["000319327700089"]},"language":[{"iso":"eng"}],"_id":"2827","abstract":[{"text":"Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology.","lang":"eng"}],"citation":{"apa":"Du, Y., Tejos, R., Beck, M., Himschoot, E., Li, H., Robatzek, S., … Friml, J. (2013). Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1220205110\">https://doi.org/10.1073/pnas.1220205110</a>","mla":"Du, Yunlong, et al. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic Protein Trafficking.” <i>PNAS</i>, vol. 110, no. 19, National Academy of Sciences, 2013, pp. 7946–51, doi:<a href=\"https://doi.org/10.1073/pnas.1220205110\">10.1073/pnas.1220205110</a>.","short":"Y. Du, R. Tejos, M. Beck, E. Himschoot, H. Li, S. Robatzek, S. Vanneste, J. Friml, PNAS 110 (2013) 7946–7951.","ieee":"Y. Du <i>et al.</i>, “Salicylic acid interferes with clathrin-mediated endocytic protein trafficking,” <i>PNAS</i>, vol. 110, no. 19. National Academy of Sciences, pp. 7946–7951, 2013.","chicago":"Du, Yunlong, Ricardo Tejos, Martina Beck, Ellie Himschoot, Hongjiang Li, Silke Robatzek, Steffen Vanneste, and Jiří Friml. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic Protein Trafficking.” <i>PNAS</i>. National Academy of Sciences, 2013. <a href=\"https://doi.org/10.1073/pnas.1220205110\">https://doi.org/10.1073/pnas.1220205110</a>.","ama":"Du Y, Tejos R, Beck M, et al. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. <i>PNAS</i>. 2013;110(19):7946-7951. doi:<a href=\"https://doi.org/10.1073/pnas.1220205110\">10.1073/pnas.1220205110</a>","ista":"Du Y, Tejos R, Beck M, Himschoot E, Li H, Robatzek S, Vanneste S, Friml J. 2013. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. PNAS. 110(19), 7946–7951."},"day":"07","status":"public","page":"7946 - 7951","scopus_import":"1","issue":"19","isi":1,"date_updated":"2025-09-29T13:52:38Z","publication":"PNAS","oa_version":"Submitted Version","date_created":"2018-12-11T11:59:48Z","title":"Salicylic acid interferes with clathrin-mediated endocytic protein trafficking","publication_status":"published","publist_id":"3972","publisher":"National Academy of Sciences","article_processing_charge":"No","corr_author":"1","pmid":1,"doi":"10.1073/pnas.1220205110","oa":1,"department":[{"_id":"JiFr"}],"month":"05","project":[{"_id":"2574781E-B435-11E9-9278-68D0E5697425","name":"Koerber Prize"}],"author":[{"first_name":"Yunlong","full_name":"Du, Yunlong","last_name":"Du"},{"first_name":"Ricardo","last_name":"Tejos","full_name":"Tejos, Ricardo"},{"full_name":"Beck, Martina","last_name":"Beck","first_name":"Martina"},{"first_name":"Ellie","last_name":"Himschoot","full_name":"Himschoot, Ellie"},{"first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660","last_name":"Li","full_name":"Li, Hongjiang"},{"first_name":"Silke","last_name":"Robatzek","full_name":"Robatzek, Silke"},{"first_name":"Steffen","last_name":"Vanneste","full_name":"Vanneste, Steffen"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí"}],"volume":110},{"month":"02","publication":"Cell Research","date_updated":"2021-01-12T07:00:27Z","author":[{"full_name":"Hongjiang Li","last_name":"Li","first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660"},{"full_name":"Xu, Tongda","last_name":"Xu","first_name":"Tongda"},{"last_name":"Lin","full_name":"Lin, Deshu","first_name":"Deshu"},{"first_name":"Mingzhang","full_name":"Wen, Mingzhang","last_name":"Wen"},{"first_name":"Mingtang","full_name":"Xie, Mingtang","last_name":"Xie"},{"last_name":"Duclercq","full_name":"Duclercq, Jérôme","first_name":"Jérôme"},{"last_name":"Bielach","full_name":"Bielach, Agnieszka","first_name":"Agnieszka"},{"last_name":"Kim","full_name":"Kim, Jungmook","first_name":"Jungmook"},{"first_name":"G Venugopala","last_name":"Reddy","full_name":"Reddy, G Venugopala"},{"first_name":"Jianru","full_name":"Zuo, Jianru","last_name":"Zuo"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Eva Benková"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Jirí Friml","last_name":"Friml"},{"last_name":"Guo","full_name":"Guo, Hongwei","first_name":"Hongwei"},{"last_name":"Yang","full_name":"Yang, Zhenbiao","first_name":"Zhenbiao"}],"volume":23,"acknowledgement":"is work was supported by grants from the US National Institute of General Medical Sciences (GM081451 and GM081451-03S2) to ZY. We thank National Science Foundation grant (IOS-1147250) to GVR and MX. HL and DL were partially supported by the Chinese Scholarship Council.","oa":1,"doi":"10.1038/cr.2012.146","issue":"2","extern":1,"publisher":"Nature Publishing Group","status":"public","page":"290 - 299","citation":{"ista":"Li H, Xu T, Lin D, Wen M, Xie M, Duclercq J, Bielach A, Kim J, Reddy GV, Zuo J, Benková E, Friml J, Guo H, Yang Z. 2013. Cytokinin signaling regulates pavement cell morphogenesis in Arabidopsis. Cell Research. 23(2), 290–299.","ama":"Li H, Xu T, Lin D, et al. Cytokinin signaling regulates pavement cell morphogenesis in Arabidopsis. <i>Cell Research</i>. 2013;23(2):290-299. doi:<a href=\"https://doi.org/10.1038/cr.2012.146\">10.1038/cr.2012.146</a>","chicago":"Li, Hongjiang, Tongda Xu, Deshu Lin, Mingzhang Wen, Mingtang Xie, Jérôme Duclercq, Agnieszka Bielach, et al. “Cytokinin Signaling Regulates Pavement Cell Morphogenesis in Arabidopsis.” <i>Cell Research</i>. Nature Publishing Group, 2013. <a href=\"https://doi.org/10.1038/cr.2012.146\">https://doi.org/10.1038/cr.2012.146</a>.","ieee":"H. Li <i>et al.</i>, “Cytokinin signaling regulates pavement cell morphogenesis in Arabidopsis,” <i>Cell Research</i>, vol. 23, no. 2. Nature Publishing Group, pp. 290–299, 2013.","short":"H. Li, T. Xu, D. Lin, M. Wen, M. Xie, J. Duclercq, A. Bielach, J. Kim, G.V. Reddy, J. Zuo, E. Benková, J. Friml, H. Guo, Z. Yang, Cell Research 23 (2013) 290–299.","mla":"Li, Hongjiang, et al. “Cytokinin Signaling Regulates Pavement Cell Morphogenesis in Arabidopsis.” <i>Cell Research</i>, vol. 23, no. 2, Nature Publishing Group, 2013, pp. 290–99, doi:<a href=\"https://doi.org/10.1038/cr.2012.146\">10.1038/cr.2012.146</a>.","apa":"Li, H., Xu, T., Lin, D., Wen, M., Xie, M., Duclercq, J., … Yang, Z. (2013). Cytokinin signaling regulates pavement cell morphogenesis in Arabidopsis. <i>Cell Research</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/cr.2012.146\">https://doi.org/10.1038/cr.2012.146</a>"},"day":"01","type":"journal_article","publist_id":"3881","quality_controlled":0,"publication_status":"published","_id":"2881","abstract":[{"text":"The puzzle piece-shaped Arabidopsis leaf pavement cells (PCs) with interdigitated lobes and indents is a good model system to investigate the mechanisms that coordinate cell polarity and shape formation within a tissue. Auxin has been shown to coordinate the interdigitation by activating ROP GTPase-dependent signaling pathways. To identify additional components or mechanisms, we screened for mutants with abnormal PC morphogenesis and found that cytokinin signaling regulates the PC interdigitation pattern. Reduction in cytokinin accumulation and defects in cytokinin signaling (such as in ARR7-over-expressing lines, the ahk3cre1 cytokinin receptor mutant, and the ahp12345 cytokinin signaling mutant) enhanced PC interdigitation, whereas over-production of cytokinin and over-activation of cytokinin signaling in an ARR20 over-expression line delayed or abolished PC interdigitation throughout the cotyledon. Genetic and biochemical analyses suggest that cytokinin signaling acts upstream of ROPs to suppress the formation of interdigitated pattern. Our results provide novel mechanistic understanding of the pathways controlling PC shape and uncover a new role for cytokinin signaling in cell morphogenesis.","lang":"eng"}],"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567823/","open_access":"1"}],"date_published":"2013-02-01T00:00:00Z","date_created":"2018-12-11T12:00:07Z","title":"Cytokinin signaling regulates pavement cell morphogenesis in Arabidopsis","year":"2013","intvolume":"        23"},{"doi":"10.1016/j.cub.2012.05.019","month":"07","volume":22,"author":[{"first_name":"Deshu","last_name":"Lin","full_name":"Lin, Deshu"},{"last_name":"Nagawa","full_name":"Nagawa, Shingo","first_name":"Shingo"},{"last_name":"Chen","full_name":"Chen, Jisheng","first_name":"Jisheng"},{"first_name":"Lingyan","full_name":"Cao, Lingyan","last_name":"Cao"},{"id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","first_name":"Xu","last_name":"Chen","full_name":"Xu Chen"},{"first_name":"Tongda","last_name":"Xu","full_name":"Xu, Tongda"},{"id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","first_name":"Hongjiang","orcid":"0000-0001-5039-9660","full_name":"Hongjiang Li","last_name":"Li"},{"last_name":"Dhonukshe","full_name":"Dhonukshe, Pankaj","first_name":"Pankaj"},{"first_name":"Chizuko","last_name":"Yamamuro","full_name":"Yamamuro, Chizuko"},{"last_name":"Friml","full_name":"Jirí Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"first_name":"Ben","full_name":"Scheres, Ben","last_name":"Scheres"},{"first_name":"Ying","last_name":"Fu","full_name":"Fu, Ying"},{"first_name":"Zhenbiao","last_name":"Yang","full_name":"Yang, Zhenbiao"}],"publication":"Current Biology","date_updated":"2021-01-12T07:41:08Z","page":"1319 - 1325","status":"public","publisher":"Cell Press","extern":1,"issue":"14","quality_controlled":0,"publication_status":"published","publist_id":"3588","type":"journal_article","_id":"3111","abstract":[{"text":"PIN-FORMED (PIN) protein-mediated auxin polar transport is critically important for development, pattern formation, and morphogenesis in plants. Auxin has been implicated in the regulation of polar auxin transport by inhibiting PIN endocytosis [1, 2], but how auxin regulates this process is poorly understood. Our genetic screen identified the Arabidopsis SPIKE1 (SPK1) gene whose loss-of-function mutations increased lateral root density and retarded gravitropic responses, as do pin2 knockout mutations [3]. SPK1 belongs to the conserved DHR2-Dock family of Rho guanine nucleotide exchange factors [4-6]. The spk1 mutations induced PIN2 internalization that was not suppressed by auxin, as did the loss-of-function mutations for Rho-like GTPase from Plants 6 (ROP6)-GTPase or its effector RIC1. Furthermore, SPK1 was required for auxin induction of ROP6 activation. Our results have established a Rho GTPase-based auxin signaling pathway that maintains PIN2 polar distribution to the plasma membrane via inhibition of its internalization in Arabidopsis roots. Our findings provide new insights into signaling mechanisms that underlie the regulation of the dynamic trafficking of PINs required for long-distance auxin transport and that link auxin signaling to PIN-mediated pattern formation and morphogenesis.","lang":"eng"}],"citation":{"short":"D. Lin, S. Nagawa, J. Chen, L. Cao, X. Chen, T. Xu, H. Li, P. Dhonukshe, C. Yamamuro, J. Friml, B. Scheres, Y. Fu, Z. Yang, Current Biology 22 (2012) 1319–1325.","ieee":"D. Lin <i>et al.</i>, “A ROP GTPase dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots,” <i>Current Biology</i>, vol. 22, no. 14. Cell Press, pp. 1319–1325, 2012.","mla":"Lin, Deshu, et al. “A ROP GTPase Dependent Auxin Signaling Pathway Regulates the Subcellular Distribution of PIN2 in Arabidopsis Roots.” <i>Current Biology</i>, vol. 22, no. 14, Cell Press, 2012, pp. 1319–25, doi:<a href=\"https://doi.org/10.1016/j.cub.2012.05.019\">10.1016/j.cub.2012.05.019</a>.","apa":"Lin, D., Nagawa, S., Chen, J., Cao, L., Chen, X., Xu, T., … Yang, Z. (2012). A ROP GTPase dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2012.05.019\">https://doi.org/10.1016/j.cub.2012.05.019</a>","ista":"Lin D, Nagawa S, Chen J, Cao L, Chen X, Xu T, Li H, Dhonukshe P, Yamamuro C, Friml J, Scheres B, Fu Y, Yang Z. 2012. A ROP GTPase dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots. Current Biology. 22(14), 1319–1325.","ama":"Lin D, Nagawa S, Chen J, et al. A ROP GTPase dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots. <i>Current Biology</i>. 2012;22(14):1319-1325. doi:<a href=\"https://doi.org/10.1016/j.cub.2012.05.019\">10.1016/j.cub.2012.05.019</a>","chicago":"Lin, Deshu, Shingo Nagawa, Jisheng Chen, Lingyan Cao, Xu Chen, Tongda Xu, Hongjiang Li, et al. “A ROP GTPase Dependent Auxin Signaling Pathway Regulates the Subcellular Distribution of PIN2 in Arabidopsis Roots.” <i>Current Biology</i>. Cell Press, 2012. <a href=\"https://doi.org/10.1016/j.cub.2012.05.019\">https://doi.org/10.1016/j.cub.2012.05.019</a>."},"day":"24","year":"2012","intvolume":"        22","title":"A ROP GTPase dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots","date_created":"2018-12-11T12:01:27Z","date_published":"2012-07-24T00:00:00Z"},{"quality_controlled":"1","publication_status":"published","type":"journal_article","publist_id":"4445","_id":"2458","abstract":[{"text":"Initiation and successive development of organs induce mechanical stresses at the cellular level. Using the tomato shoot apex, a new study now proposes that mechanical strain regulates the plasma membrane abundance of the PIN1 auxin transporter, thereby reinforcing a positive feed-back loop between growth and auxin accumulation.","lang":"eng"}],"language":[{"iso":"eng"}],"citation":{"short":"H. Li, J. Friml, W. Grunewald, Current Biology 22 (2012) R635–R637.","ieee":"H. Li, J. Friml, and W. Grunewald, “Cell polarity: Stretching prevents developmental cramps,” <i>Current Biology</i>, vol. 22, no. 16. Cell Press, pp. R635–R637, 2012.","mla":"Li, Hongjiang, et al. “Cell Polarity: Stretching Prevents Developmental Cramps.” <i>Current Biology</i>, vol. 22, no. 16, Cell Press, 2012, pp. R635–37, doi:<a href=\"https://doi.org/10.1016/j.cub.2012.06.053\">10.1016/j.cub.2012.06.053</a>.","apa":"Li, H., Friml, J., &#38; Grunewald, W. (2012). Cell polarity: Stretching prevents developmental cramps. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2012.06.053\">https://doi.org/10.1016/j.cub.2012.06.053</a>","ista":"Li H, Friml J, Grunewald W. 2012. Cell polarity: Stretching prevents developmental cramps. Current Biology. 22(16), R635–R637.","ama":"Li H, Friml J, Grunewald W. Cell polarity: Stretching prevents developmental cramps. <i>Current Biology</i>. 2012;22(16):R635-R637. doi:<a href=\"https://doi.org/10.1016/j.cub.2012.06.053\">10.1016/j.cub.2012.06.053</a>","chicago":"Li, Hongjiang, Jiří Friml, and Wim Grunewald. “Cell Polarity: Stretching Prevents Developmental Cramps.” <i>Current Biology</i>. Cell Press, 2012. <a href=\"https://doi.org/10.1016/j.cub.2012.06.053\">https://doi.org/10.1016/j.cub.2012.06.053</a>."},"day":"21","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","year":"2012","intvolume":"        22","oa_version":"None","date_created":"2018-12-11T11:57:47Z","title":"Cell polarity: Stretching prevents developmental cramps","date_published":"2012-08-21T00:00:00Z","doi":"10.1016/j.cub.2012.06.053","month":"08","author":[{"last_name":"Li","full_name":"Li, Hongjiang","orcid":"0000-0001-5039-9660","first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","full_name":"Friml, Jirí","last_name":"Friml"},{"full_name":"Grunewald, Wim","last_name":"Grunewald","first_name":"Wim"}],"volume":22,"publication":"Current Biology","date_updated":"2021-01-12T06:57:36Z","page":"R635 - R637","status":"public","publisher":"Cell Press","extern":"1","issue":"16"},{"abstract":[{"lang":"eng","text":"Within a multicellular tissue cells may coordinately form a singular or multiple polar axes, but it is unclear whether a common mechanism governs different types of polar axis formation. The phosphorylation status of PIN proteins, which is directly affected by the PINOID (PID) protein kinase and the PP2A protein phosphatase, is known to regulate the apical-basal polarity of PIN localization in bipolar cells of roots and shoot apices. Here, we provide evidence that the phosphorylation status-mediated PIN polarity switch is widely used to modulate cellular processes in Arabidopsis including multipolar pavement cells (PC) with interdigitated lobes and indentations. The degree of PC interdigitation was greatly reduced either when the FYPP1 gene, which encodes a PP2A called phytochrome-associated serine/threonine protein phosphatase, was knocked out or when the PID gene was overexpressed (35S:PID). These genetic modifications caused PIN1 localization to switch from lobe to indentation regions. The PP2A and PID mediated switching of PIN1 localization is strikingly similar to their regulation of the apical-basal polarity switch of PIN proteins in other cells. Our findings suggest a common mechanism for the regulation of PIN1 polarity formation, a fundamental cellular process that is crucial for pattern formation both at the tissue/organ and cellular levels."}],"_id":"2454","type":"journal_article","publist_id":"4449","publication_status":"published","quality_controlled":0,"day":"01","citation":{"ieee":"H. Li <i>et al.</i>, “Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis,” <i>Cell Research</i>, vol. 21, no. 6. Nature Publishing Group, pp. 970–978, 2011.","short":"H. Li, D. Lin, P. Dhonukshe, S. Nagawa, D. Chen, J. Friml, B. Scheres, H. Guo, Z. Yang, Cell Research 21 (2011) 970–978.","mla":"Li, Hongjiang, et al. “Phosphorylation Switch Modulates the Interdigitated Pattern of PIN1 Localization and Cell Expansion in Arabidopsis Leaf Epidermis.” <i>Cell Research</i>, vol. 21, no. 6, Nature Publishing Group, 2011, pp. 970–78, doi:<a href=\"https://doi.org/10.1038/cr.2011.49\">10.1038/cr.2011.49</a>.","apa":"Li, H., Lin, D., Dhonukshe, P., Nagawa, S., Chen, D., Friml, J., … Yang, Z. (2011). Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis. <i>Cell Research</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/cr.2011.49\">https://doi.org/10.1038/cr.2011.49</a>","ista":"Li H, Lin D, Dhonukshe P, Nagawa S, Chen D, Friml J, Scheres B, Guo H, Yang Z. 2011. Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis. Cell Research. 21(6), 970–978.","ama":"Li H, Lin D, Dhonukshe P, et al. Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis. <i>Cell Research</i>. 2011;21(6):970-978. doi:<a href=\"https://doi.org/10.1038/cr.2011.49\">10.1038/cr.2011.49</a>","chicago":"Li, Hongjiang, Deshu Lin, Pankaj Dhonukshe, Shingo Nagawa, Dandan Chen, Jiří Friml, Ben Scheres, Hongwei Guo, and Zhenbiao Yang. “Phosphorylation Switch Modulates the Interdigitated Pattern of PIN1 Localization and Cell Expansion in Arabidopsis Leaf Epidermis.” <i>Cell Research</i>. Nature Publishing Group, 2011. <a href=\"https://doi.org/10.1038/cr.2011.49\">https://doi.org/10.1038/cr.2011.49</a>."},"intvolume":"        21","year":"2011","date_published":"2011-06-01T00:00:00Z","date_created":"2018-12-11T11:57:45Z","title":"Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203702/","open_access":"1"}],"oa":1,"doi":"10.1038/cr.2011.49","publication":"Cell Research","date_updated":"2021-01-12T06:57:35Z","volume":21,"author":[{"last_name":"Li","full_name":"Hongjiang Li","first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660"},{"first_name":"Deshu","full_name":"Lin, Deshu","last_name":"Lin"},{"last_name":"Dhonukshe","full_name":"Dhonukshe, Pankaj B","first_name":"Pankaj"},{"full_name":"Nagawa, Shingo","last_name":"Nagawa","first_name":"Shingo"},{"first_name":"Dandan","full_name":"Chen, Dandan","last_name":"Chen"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Jirí Friml","last_name":"Friml"},{"first_name":"Ben","last_name":"Scheres","full_name":"Scheres, Ben"},{"last_name":"Guo","full_name":"Guo, Hongwei","first_name":"Hongwei"},{"full_name":"Yang, Zhenbiao","last_name":"Yang","first_name":"Zhenbiao"}],"month":"06","publisher":"Nature Publishing Group","status":"public","page":"970 - 978","issue":"6","extern":1}]