[{"OA_type":"hybrid","corr_author":"1","ddc":["580"],"date_published":"2025-04-01T00:00:00Z","author":[{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","first_name":"Michelle C"},{"first_name":"Sven M","last_name":"Truckenbrodt","full_name":"Truckenbrodt, Sven M","id":"45812BD4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kreuzinger","first_name":"Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87","full_name":"Kreuzinger, Caroline"},{"first_name":"Syamala","last_name":"Inumella","full_name":"Inumella, Syamala","id":"F8660870-D756-11E9-98C5-34DFE5697425","orcid":"0009-0002-5890-120X"},{"id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","full_name":"Vistunou, Vitali","last_name":"Vistunou","first_name":"Vitali"},{"first_name":"Christoph M","last_name":"Sommer","orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Mojtaba","last_name":"Tavakoli","orcid":"0000-0002-7667-6854","full_name":"Tavakoli, Mojtaba","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87"},{"id":"40E7F008-F248-11E8-B48F-1D18A9856A87","full_name":"Agudelo Duenas, Nathalie","last_name":"Agudelo Duenas","first_name":"Nathalie"},{"full_name":"Vorlaufer, Jakob","id":"937696FA-C996-11E9-8C7C-CF13E6697425","orcid":"0009-0000-7590-3501","first_name":"Jakob","last_name":"Vorlaufer"},{"last_name":"Jahr","first_name":"Wiebke","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","full_name":"Jahr, Wiebke","orcid":"0000-0003-0201-2315"},{"first_name":"Marek","last_name":"Randuch","full_name":"Randuch, Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"first_name":"Alexander J","last_name":"Johnson","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843"},{"last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"},{"first_name":"Johann G","last_name":"Danzl","orcid":"0000-0001-8559-3973","full_name":"Danzl, Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87"}],"intvolume":"        37","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"has_accepted_license":"1","publication_status":"published","pmid":1,"status":"public","date_updated":"2026-06-10T08:30:19Z","article_type":"original","publisher":"Oxford University Press","year":"2025","PlanS_conform":"1","day":"01","file":[{"creator":"dernst","file_size":53904111,"content_type":"application/pdf","date_updated":"2025-07-31T07:03:43Z","date_created":"2025-07-31T07:03:43Z","checksum":"9d3f8218ff37a29f29c48a7bbe831bd3","relation":"main_file","access_level":"open_access","success":1,"file_name":"2025_PlantCell_Gallei.pdf","file_id":"20092"}],"ec_funded":1,"volume":37,"abstract":[{"text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. ","lang":"eng"}],"publication":"The Plant Cell","file_date_updated":"2025-07-31T07:03:43Z","article_number":"koaf006","title":"Super-resolution expansion microscopy in plant roots","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}],"external_id":{"pmid":["39792900"],"isi":["001462763100001"]},"article_processing_charge":"Yes (via OA deal)","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","name":"International IST Doctoral Program","call_identifier":"H2020"},{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","_id":"269B5B22-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 679-2018"},{"name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5","grant_number":"26137"},{"name":"Molecular Drug Targets","call_identifier":"FWF","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24"}],"acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","_id":"19003","related_material":{"record":[{"id":"18689","status":"public","relation":"earlier_version"},{"id":"18837","relation":"research_data","status":"public"}]},"publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"date_created":"2025-02-05T06:52:06Z","doi":"10.1093/plcell/koaf006","type":"journal_article","month":"04","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006.","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>.","ieee":"M. C. Gallei <i>et al.</i>, “Super-resolution expansion microscopy in plant roots,” <i>The Plant Cell</i>, vol. 37, no. 4. Oxford University Press, 2025.","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. 2025;37(4). doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>."},"scopus_import":"1","OA_place":"publisher","quality_controlled":"1","language":[{"iso":"eng"}],"issue":"4","oa":1,"oa_version":"Published Version","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}]},{"publication_status":"draft","status":"public","publisher":"Cold Spring Harbor Laboratory","date_updated":"2026-06-18T18:13:35Z","day":"02","year":"2025","date_published":"2025-03-02T00:00:00Z","ddc":["580"],"corr_author":"1","OA_type":"green","author":[{"first_name":"Aline","last_name":"Monzer","full_name":"Monzer, Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425"},{"first_name":"Ewa","last_name":"Mazur","full_name":"Mazur, Ewa"},{"first_name":"Lesia","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237","full_name":"Rodriguez Solovey, Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Gallei","first_name":"Michelle C","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C"},{"full_name":"Zou, Minxia","id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","first_name":"Minxia","last_name":"Zou"},{"full_name":"Smejkal, Michael","id":"79a5a1be-04a3-11f0-ba18-a1730e0b58e9","first_name":"Michael","last_name":"Smejkal"},{"first_name":"Ema","last_name":"Cervenova","full_name":"Cervenova, Ema","id":"9f185b95-04a3-11f0-8245-f5e32eeb470f"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"has_accepted_license":"1","_id":"19398","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"19395"}]},"date_created":"2025-03-12T14:28:53Z","doi":"10.1101/2025.02.28.640727","main_file_link":[{"url":"https://doi.org/10.1101/2025.02.28.640727","open_access":"1"}],"acknowledgement":"We deeply appreciate M. Wrzaczek’s constructive input and insightful discussions, which significantly enriched this work. We thank L. Fiedler for helping with the heat map and for the discussions. We also thank the facilities at ISTA, the imaging and optics (IOF) and Lab Support (LSF) facilities for their service and assistance.","citation":{"apa":"Monzer, A., Mazur, E., Rodriguez Solovey, L., Gallei, M. C., Zou, M., Smejkal, M., … Friml, J. (n.d.). TMK interacting network of receptor like kinases for auxin canalization and beyond. <i>bioRxiv</i>. Cold Spring Harbor Laboratory. <a href=\"https://doi.org/10.1101/2025.02.28.640727\">https://doi.org/10.1101/2025.02.28.640727</a>","ista":"Monzer A, Mazur E, Rodriguez Solovey L, Gallei MC, Zou M, Smejkal M, Cervenova E, Friml J. TMK interacting network of receptor like kinases for auxin canalization and beyond. bioRxiv, <a href=\"https://doi.org/10.1101/2025.02.28.640727\">10.1101/2025.02.28.640727</a>.","chicago":"Monzer, Aline, Ewa Mazur, Lesia Rodriguez Solovey, Michelle C Gallei, Minxia Zou, Michael Smejkal, Ema Cervenova, and Jiří Friml. “TMK Interacting Network of Receptor like Kinases for Auxin Canalization and Beyond.” <i>BioRxiv</i>. Cold Spring Harbor Laboratory, n.d. <a href=\"https://doi.org/10.1101/2025.02.28.640727\">https://doi.org/10.1101/2025.02.28.640727</a>.","ama":"Monzer A, Mazur E, Rodriguez Solovey L, et al. TMK interacting network of receptor like kinases for auxin canalization and beyond. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.02.28.640727\">10.1101/2025.02.28.640727</a>","mla":"Monzer, Aline, et al. “TMK Interacting Network of Receptor like Kinases for Auxin Canalization and Beyond.” <i>BioRxiv</i>, Cold Spring Harbor Laboratory, doi:<a href=\"https://doi.org/10.1101/2025.02.28.640727\">10.1101/2025.02.28.640727</a>.","ieee":"A. Monzer <i>et al.</i>, “TMK interacting network of receptor like kinases for auxin canalization and beyond,” <i>bioRxiv</i>. Cold Spring Harbor Laboratory.","short":"A. Monzer, E. Mazur, L. Rodriguez Solovey, M.C. Gallei, M. Zou, M. Smejkal, E. Cervenova, J. Friml, BioRxiv (n.d.)."},"type":"preprint","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"03","language":[{"iso":"eng"}],"OA_place":"repository","department":[{"_id":"GradSch"},{"_id":"JiFr"},{"_id":"EvBe"}],"oa":1,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Receptor-like kinases (RLKs), particularly the Transmembrane Kinase (TMK) family, play essential roles in signaling and development, with TMKs being key components of auxin perception and downstream phosphorylation events. While TMKs’ involvement in auxin canalization, a process essential for vasculature formation and regeneration, has been established, nonetheless, the additional signaling and regulatory partners remain poorly understood. In this study, we identify and characterize seven leucine-rich repeat RLKs (TINT1–TINT7) as novel interactors of TMK1, revealing their diverse evolutionary, structural, and functional characteristics. Our results show that TINTs interact with TMK1 and highlight their roles in regulating various developmental processes. Majority of TINTs contributes, together with TMK1, to auxin canalization, with TINT5 linking TMK1 to other canalization component CAMEL. Beyond canalization, we also establish the role of TINT-TMK1 interactions in processes such as stomatal movement and the hypocotyl’s gravitropic response. These findings suggest that TINTs, through their interaction with TMK1, are integral components of various signaling networks, contributing to both auxin canalization and broader plant development."}],"publication":"bioRxiv","title":"TMK interacting network of receptor like kinases for auxin canalization and beyond","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"article_processing_charge":"No"},{"author":[{"full_name":"Huang, R","last_name":"Huang","first_name":"R"},{"full_name":"Wang, J","last_name":"Wang","first_name":"J"},{"first_name":"M","last_name":"Chang","full_name":"Chang, M"},{"full_name":"Tang, W","last_name":"Tang","first_name":"W"},{"last_name":"Yu","first_name":"Y","full_name":"Yu, Y"},{"first_name":"Y","last_name":"Zhang","full_name":"Zhang, Y"},{"full_name":"Peng, Y","last_name":"Peng","first_name":"Y"},{"first_name":"Y","last_name":"Wang","full_name":"Wang, Y"},{"full_name":"Guo, Y","first_name":"Y","last_name":"Guo"},{"last_name":"Lu","first_name":"T","full_name":"Lu, T"},{"full_name":"Cao, Y","first_name":"Y","last_name":"Cao"},{"first_name":"Y","last_name":"Zhou","full_name":"Zhou, Y"},{"first_name":"Q","last_name":"Zhang","full_name":"Zhang, Q"},{"last_name":"Huang","first_name":"Y","full_name":"Huang, Y"},{"full_name":"Wu, A","first_name":"A","last_name":"Wu"},{"first_name":"L","last_name":"Ren","full_name":"Ren, L"},{"first_name":"Michelle C","last_name":"Gallei","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Dong, J","last_name":"Dong","first_name":"J"},{"last_name":"Chen","first_name":"H","full_name":"Chen, H"},{"full_name":"He, J","last_name":"He","first_name":"J"},{"full_name":"Wen, M","last_name":"Wen","first_name":"M"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"},{"full_name":"Sun, L","last_name":"Sun","first_name":"L"},{"last_name":"Xiong","first_name":"Y","full_name":"Xiong, Y"},{"full_name":"Yang, Z","first_name":"Z","last_name":"Yang"},{"first_name":"T","last_name":"Xu","full_name":"Xu, T"}],"OA_type":"closed access","date_published":"2025-10-02T00:00:00Z","year":"2025","day":"02","date_updated":"2025-11-24T13:43:08Z","article_type":"original","publisher":"Elsevier","publication_status":"epub_ahead","pmid":1,"status":"public","external_id":{"pmid":["41043435"]},"page":"S1534-5807(25)00569-6","article_processing_charge":"No","title":"TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis","abstract":[{"text":"The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis.","lang":"eng"}],"publication":"Developmental Cell","oa_version":"None","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"quality_controlled":"1","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"10","citation":{"chicago":"Huang, R, J Wang, M Chang, W Tang, Y Yu, Y Zhang, Y Peng, et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">https://doi.org/10.1016/j.devcel.2025.09.009</a>.","ama":"Huang R, Wang J, Chang M, et al. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. <i>Developmental Cell</i>. 2025:S1534-5807(25)00569-6. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">10.1016/j.devcel.2025.09.009</a>","mla":"Huang, R., et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” <i>Developmental Cell</i>, Elsevier, 2025, pp. S1534-5807(25)00569-6, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">10.1016/j.devcel.2025.09.009</a>.","ieee":"R. Huang <i>et al.</i>, “TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis,” <i>Developmental Cell</i>. Elsevier, pp. S1534-5807(25)00569–6, 2025.","short":"R. Huang, J. Wang, M. Chang, W. Tang, Y. Yu, Y. Zhang, Y. Peng, Y. Wang, Y. Guo, T. Lu, Y. Cao, Y. Zhou, Q. Zhang, Y. Huang, A. Wu, L. Ren, M.C. Gallei, J. Dong, H. Chen, J. He, M. Wen, J. Friml, L. Sun, Y. Xiong, Z. Yang, T. Xu, Developmental Cell (2025) S1534-5807(25)00569–6.","apa":"Huang, R., Wang, J., Chang, M., Tang, W., Yu, Y., Zhang, Y., … Xu, T. (2025). TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">https://doi.org/10.1016/j.devcel.2025.09.009</a>","ista":"Huang R, Wang J, Chang M, Tang W, Yu Y, Zhang Y, Peng Y, Wang Y, Guo Y, Lu T, Cao Y, Zhou Y, Zhang Q, Huang Y, Wu A, Ren L, Gallei MC, Dong J, Chen H, He J, Wen M, Friml J, Sun L, Xiong Y, Yang Z, Xu T. 2025. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell., S1534-5807(25)00569–6."},"scopus_import":"1","acknowledgement":"We thank Lukáš Fiedler‬ for helping with the writing. This work was supported by the National Key Research and Development Program of China (2023YFA0913500) to T.X., R.H., Y.Y., Y.X., and M.W. and by the National Natural Science Foundation of China grants to T.X. (32130010), Z.Y. (3241101698), and R.H. (32070309 and 32470276) and startup funds from the Fujian Agriculture and Forestry University and the Shanghai Plant Stress Biology Center, Chinese Academy of Sciences to T.X.","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"_id":"20636","date_created":"2025-11-12T10:03:39Z","doi":"10.1016/j.devcel.2025.09.009"},{"file":[{"access_level":"open_access","date_created":"2024-12-03T11:08:09Z","checksum":"a11feea4b1677df76b632eca04bfc1dd","relation":"main_file","content_type":"application/pdf","date_updated":"2024-12-03T11:08:09Z","file_size":3308945,"creator":"dernst","file_id":"18612","file_name":"2024_MolecularPlant_Kralova.pdf","success":1}],"year":"2024","day":"02","date_updated":"2025-09-08T14:46:45Z","article_type":"original","publisher":"Elsevier","status":"public","pmid":1,"publication_status":"published","has_accepted_license":"1","intvolume":"        17","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"isi":1,"author":[{"first_name":"Michaela","last_name":"Králová","full_name":"Králová, Michaela"},{"first_name":"Ivona","last_name":"Kubalová","full_name":"Kubalová, Ivona"},{"last_name":"Hajný","first_name":"Jakub","full_name":"Hajný, Jakub"},{"last_name":"Kubiasova","first_name":"Karolina","id":"946011F4-3E71-11EA-860B-C7A73DDC885E","full_name":"Kubiasova, Karolina","orcid":"0000-0001-5630-9419"},{"first_name":"Karolína","last_name":"Vagaská","full_name":"Vagaská, Karolína"},{"first_name":"Zengxiang","last_name":"Ge","full_name":"Ge, Zengxiang","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","orcid":"0000-0001-9381-3577"},{"full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368","first_name":"Michelle C","last_name":"Gallei"},{"last_name":"Semerádová","first_name":"Hana","id":"42FE702E-F248-11E8-B48F-1D18A9856A87","full_name":"Semerádová, Hana"},{"full_name":"Kuchařová, Anna","last_name":"Kuchařová","first_name":"Anna"},{"full_name":"Hönig, Martin","first_name":"Martin","last_name":"Hönig"},{"last_name":"Monzer","first_name":"Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","full_name":"Monzer, Aline"},{"full_name":"Kovačik, Martin","first_name":"Martin","last_name":"Kovačik"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"},{"full_name":"Novák, Ondřej","first_name":"Ondřej","last_name":"Novák"},{"last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"full_name":"Ikeda, Yoshihisa","first_name":"Yoshihisa","last_name":"Ikeda"},{"full_name":"Zalabák, David","last_name":"Zalabák","first_name":"David"}],"OA_type":"hybrid","ddc":["580"],"date_published":"2024-12-02T00:00:00Z","oa":1,"oa_version":"Published Version","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"OA_place":"publisher","issue":"12","quality_controlled":"1","language":[{"iso":"eng"}],"month":"12","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","scopus_import":"1","citation":{"mla":"Králová, Michaela, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>, vol. 17, no. 12, Elsevier, 2024, pp. 1850–65, doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>.","ieee":"M. Králová <i>et al.</i>, “A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis,” <i>Molecular Plant</i>, vol. 17, no. 12. Elsevier, pp. 1850–1865, 2024.","short":"M. Králová, I. Kubalová, J. Hajný, K. Kubiasova, K. Vagaská, Z. Ge, M.C. Gallei, H. Semerádová, A. Kuchařová, M. Hönig, A. Monzer, M. Kovačik, J. Friml, O. Novák, E. Benková, Y. Ikeda, D. Zalabák, Molecular Plant 17 (2024) 1850–1865.","chicago":"Králová, Michaela, Ivona Kubalová, Jakub Hajný, Karolina Kubiasova, Karolína Vagaská, Zengxiang Ge, Michelle C Gallei, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>.","ama":"Králová M, Kubalová I, Hajný J, et al. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. 2024;17(12):1850-1865. doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>","ista":"Králová M, Kubalová I, Hajný J, Kubiasova K, Vagaská K, Ge Z, Gallei MC, Semerádová H, Kuchařová A, Hönig M, Monzer A, Kovačik M, Friml J, Novák O, Benková E, Ikeda Y, Zalabák D. 2024. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. Molecular Plant. 17(12), 1850–1865.","apa":"Králová, M., Kubalová, I., Hajný, J., Kubiasova, K., Vagaská, K., Ge, Z., … Zalabák, D. (2024). A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>"},"acknowledgement":"We dedicate this paper to the deceased Petr Galuszka for his inspiration and support of our project. We thank Prof. Peter Hedden for constructive criticism of the manuscript and English editing. No conflict of interest is declared.","date_created":"2024-11-28T11:13:35Z","doi":"10.1016/j.molp.2024.11.001","_id":"18596","publication_identifier":{"issn":["1674-2052"]},"article_processing_charge":"Yes (in subscription journal)","page":"1850-1865","external_id":{"pmid":["39501563"],"isi":["001373778300001"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"title":"A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis","abstract":[{"lang":"eng","text":"Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsis thaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int7 results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis."}],"publication":"Molecular Plant","volume":17,"file_date_updated":"2024-12-03T11:08:09Z"},{"author":[{"last_name":"Gallei","first_name":"Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368"},{"last_name":"Truckenbrodt","first_name":"Sven M","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","full_name":"Truckenbrodt, Sven M"},{"first_name":"Caroline","last_name":"Kreuzinger","full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Inumella","first_name":"Syamala","orcid":"0009-0002-5890-120X","id":"F8660870-D756-11E9-98C5-34DFE5697425","full_name":"Inumella, Syamala"},{"id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","full_name":"Vistunou, Vitali","last_name":"Vistunou","first_name":"Vitali"},{"last_name":"Sommer","first_name":"Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M"},{"last_name":"Tavakoli","first_name":"Mojtaba","orcid":"0000-0002-7667-6854","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","full_name":"Tavakoli, Mojtaba"},{"first_name":"Nathalie","last_name":"Agudelo Duenas","full_name":"Agudelo Duenas, Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87"},{"id":"937696FA-C996-11E9-8C7C-CF13E6697425","full_name":"Vorlaufer, Jakob","orcid":"0009-0000-7590-3501","last_name":"Vorlaufer","first_name":"Jakob"},{"orcid":"0000-0003-0201-2315","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","full_name":"Jahr, Wiebke","last_name":"Jahr","first_name":"Wiebke"},{"first_name":"Marek","last_name":"Randuch","full_name":"Randuch, Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"last_name":"Johnson","first_name":"Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843"},{"last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"},{"id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","last_name":"Danzl","first_name":"Johann G"}],"corr_author":"1","date_published":"2024-02-21T00:00:00Z","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"date_updated":"2026-04-07T12:56:36Z","publication_status":"draft","status":"public","year":"2024","day":"21","title":"Super-resolution expansion microscopy in plant roots","ec_funded":1,"abstract":[{"text":"Multiplexed fluorescence microscopy imaging is widely used in biomedical applications. However, simultaneous imaging of multiple fluorophores can result in spectral leaks and overlapping, which greatly degrades image quality and subsequent analysis. Existing popular spectral unmixing methods are mainly based on computational intensive linear models and the performance is heavily dependent on the reference spectra, which may greatly preclude its further applications. In this paper, we propose a deep learning-based blindly spectral unmixing method, termed AutoUnmix, to imitate the physical spectral mixing process. A tranfer learning framework is further devised to allow our AutoUnmix adapting to a variety of imaging systems without retraining the network. Our proposed method has demonstrated real-time unmixing capabilities, surpassing existing methods by up to 100-fold in terms of unmixing speed. We further validate the reconstruction performance on both synthetic datasets and biological samples. The unmixing results of AutoUnmix achieve a highest SSIM of 0.99 in both three- and four-color imaging, with nearly up to 20% higher than other popular unmixing methods. Due to the desirable property of data independency and superior blind unmixing performance, we believe AutoUnmix is a powerful tool to study the interaction process of different organelles labeled by multiple fluorophores.","lang":"eng"}],"publication":"bioRxiv","article_processing_charge":"No","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"International IST Doctoral Program","call_identifier":"H2020","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships"},{"_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24","call_identifier":"FWF","name":"Molecular Drug Targets"},{"grant_number":"ALTF 679-2018","_id":"269B5B22-B435-11E9-9278-68D0E5697425","name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"type":"preprint","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"02","citation":{"apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (n.d.). Super-resolution expansion microscopy in plant roots. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.02.21.581330\">https://doi.org/10.1101/2024.02.21.581330</a>","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. Super-resolution expansion microscopy in plant roots. bioRxiv, <a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>.","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.02.21.581330\">https://doi.org/10.1101/2024.02.21.581330</a>.","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>","ieee":"M. C. Gallei <i>et al.</i>, “Super-resolution expansion microscopy in plant roots,” <i>bioRxiv</i>. .","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>.","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, BioRxiv (n.d.)."},"main_file_link":[{"url":"https://doi.org/10.1101/2024.02.21.581330","open_access":"1"}],"acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\n\r\nThis project has received funding from the Austrian Science Fund (FWF): I 3630-B25 (J.G.D) and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 742985, J.F.). It has also received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385. S.T. has received funding as an ISTplus Fellow from the European Union’s Horizon 2020 Research and Innovation Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from an EMBO Long-Term Fellowship (grant number ALTF 679-2018). It has further received funding from the Austrian Science Fund (FWF) grant DK W1232 (M.T, N.A-D., J.G.D). W.J. received funding via a Human Frontier Science Program postdoctoral fellowship LT000557/2018.\r\n\r\nThe funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","_id":"18689","related_material":{"record":[{"relation":"later_version","status":"public","id":"19003"},{"status":"public","relation":"dissertation_contains","id":"18681"}]},"doi":"10.1101/2024.02.21.581330","date_created":"2024-12-19T12:28:00Z","oa":1,"oa_version":"Preprint","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"OA_place":"repository","language":[{"iso":"eng"}]},{"OA_place":"publisher","language":[{"iso":"eng"}],"oa":1,"oa_version":"Published Version","department":[{"_id":"GradSch"},{"_id":"JiFr"}],"_id":"11626","publication_identifier":{"issn":["2663-337X"],"isbn":["978-3-99078-019-0"]},"related_material":{"record":[{"id":"8138","relation":"part_of_dissertation","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"7142"},{"status":"public","relation":"part_of_dissertation","id":"10411"},{"relation":"part_of_dissertation","status":"public","id":"8931"},{"id":"7465","status":"public","relation":"part_of_dissertation"},{"status":"public","relation":"part_of_dissertation","id":"9287"},{"relation":"part_of_dissertation","status":"public","id":"6260"}]},"doi":"10.15479/at:ista:11626","date_created":"2022-07-20T11:21:53Z","type":"dissertation","month":"07","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","citation":{"ista":"Gallei MC. 2022. Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana. Institute of Science and Technology Austria.","apa":"Gallei, M. C. (2022). <i>Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:11626\">https://doi.org/10.15479/at:ista:11626</a>","short":"M.C. Gallei, Auxin and Strigolactone Non-Canonical Signaling Regulating Development in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2022.","ieee":"M. C. Gallei, “Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2022.","mla":"Gallei, Michelle C. <i>Auxin and Strigolactone Non-Canonical Signaling Regulating Development in Arabidopsis Thaliana</i>. Institute of Science and Technology Austria, 2022, doi:<a href=\"https://doi.org/10.15479/at:ista:11626\">10.15479/at:ista:11626</a>.","ama":"Gallei MC. Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana. 2022. doi:<a href=\"https://doi.org/10.15479/at:ista:11626\">10.15479/at:ista:11626</a>","chicago":"Gallei, Michelle C. “Auxin and Strigolactone Non-Canonical Signaling Regulating Development in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2022. <a href=\"https://doi.org/10.15479/at:ista:11626\">https://doi.org/10.15479/at:ista:11626</a>."},"degree_awarded":"PhD","supervisor":[{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"},{"last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"full_name":"Shani, Eilon","first_name":"Eilon","last_name":"Shani"}],"page":"248","article_processing_charge":"No","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"}],"ec_funded":1,"alternative_title":["ISTA Thesis"],"abstract":[{"lang":"eng","text":"Plant growth and development is well known to be both, flexible and dynamic. The high capacity for post-embryonic organ formation and tissue regeneration requires tightly regulated intercellular communication and coordinated tissue polarization. One of the most important drivers for patterning and polarity in plant development is the phytohormone auxin. Auxin has the unique characteristic to establish polarized channels for its own active directional cell to cell transport. This fascinating phenomenon is called auxin canalization. Those auxin transport channels are characterized by the expression and polar, subcellular localization of PIN auxin efflux carriers. PIN proteins have the ability to dynamically change their localization and auxin itself can affect this by interfering with trafficking. Most of the underlying molecular mechanisms of canalization still remain enigmatic. What is known so far is that canonical auxin signaling is indispensable but also other non-canonical signaling components are thought to play a role. In order to shed light into the mysteries auf auxin canalization this study revisits the branches of auxin signaling in detail. Further a new auxin analogue, PISA, is developed which triggers auxin-like responses but does not directly activate canonical transcriptional auxin signaling. We revisit the direct auxin effect on PIN trafficking where we found that, contradictory to previous observations, auxin is very specifically promoting endocytosis of PIN2 but has no overall effect on endocytosis. Further, we evaluate which cellular processes related to PIN subcellular dynamics are involved in the establishment of auxin conducting channels and the formation of vascular tissue. We are re-evaluating the function of AUXIN BINDING PROTEIN 1 (ABP1) and provide a comprehensive picture about its developmental phneotypes and involvement in auxin signaling and canalization. Lastly, we are focusing on the crosstalk between the hormone strigolactone (SL) and auxin and found that SL is interfering with essentially all processes involved in auxin canalization in a non-transcriptional manner. Lastly we identify a new way of SL perception and signaling which is emanating from mitochondria, is independent of canonical SL signaling and is modulating primary root growth."}],"file_date_updated":"2022-07-25T11:48:45Z","title":"Auxin and strigolactone non-canonical signaling regulating development in Arabidopsis thaliana","year":"2022","day":"20","file":[{"creator":"mgallei","file_size":9730864,"content_type":"application/pdf","date_updated":"2022-07-25T09:08:47Z","relation":"main_file","date_created":"2022-07-25T09:08:47Z","checksum":"bd7ac35403cf5b4b2607287d2a104b3a","access_level":"open_access","file_name":"Thesis_Gallei.pdf","file_id":"11645"},{"file_name":"Thesis_Gallei_source.docx","file_id":"11646","date_created":"2022-07-25T09:09:09Z","checksum":"a9e54fe5471ba25dc13c2150c1b8ccbb","relation":"source_file","access_level":"closed","creator":"mgallei","date_updated":"2022-07-25T09:39:58Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":19560720},{"file_name":"Thesis_Gallei_to_print.pdf","file_id":"11647","relation":"source_file","date_created":"2022-07-25T09:09:32Z","checksum":"3994f7f20058941b5bb8a16886b21e71","description":"This is the print version of the thesis including the full appendix","access_level":"closed","creator":"mgallei","file_size":24542837,"content_type":"application/pdf","date_updated":"2022-07-25T09:39:58Z"},{"content_type":"application/pdf","date_updated":"2022-07-25T11:48:45Z","file_size":15435966,"creator":"mgallei","access_level":"open_access","date_created":"2022-07-25T11:48:45Z","relation":"main_file","checksum":"f24acd3c0d864f4c6676e8b0d7bfa76b","file_id":"11650","file_name":"Thesis_Gallei_Appendix.pdf"}],"publication_status":"published","status":"public","date_updated":"2026-06-18T19:02:05Z","publisher":"Institute of Science and Technology Austria","has_accepted_license":"1","corr_author":"1","ddc":["575"],"date_published":"2022-07-20T00:00:00Z","author":[{"orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","last_name":"Gallei","first_name":"Michelle C"}]},{"article_processing_charge":"No","page":"575-581","external_id":{"isi":["000851357500002"],"pmid":["36071161"]},"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"RNA-directed DNA methylation in plant development","call_identifier":"FWF","_id":"262EF96E-B435-11E9-9278-68D0E5697425","grant_number":"P29988"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"title":"ABP1–TMK auxin perception for global phosphorylation and auxin canalization","volume":609,"ec_funded":1,"abstract":[{"lang":"eng","text":"The phytohormone auxin triggers transcriptional reprogramming through a well-characterized perception machinery in the nucleus. By contrast, mechanisms that underlie fast effects of auxin, such as the regulation of ion fluxes, rapid phosphorylation of proteins or auxin feedback on its transport, remain unclear1,2,3. Whether auxin-binding protein 1 (ABP1) is an auxin receptor has been a source of debate for decades1,4. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at an acidic pH that is typical of the apoplast. ABP1 and its plasma-membrane-localized partner, transmembrane kinase 1 (TMK1), are required for the auxin-induced ultrafast global phospho-response and for downstream processes that include the activation of H+-ATPase and accelerated cytoplasmic streaming. abp1 and tmk mutants cannot establish auxin-transporting channels and show defective auxin-induced vasculature formation and regeneration. An ABP1(M2X) variant that lacks the capacity to bind auxin is unable to complement these defects in abp1 mutants. These data indicate that ABP1 is the auxin receptor for TMK1-based cell-surface signalling, which mediates the global phospho-response and auxin canalization."}],"publication":"Nature","file_date_updated":"2023-11-02T17:12:37Z","oa_version":"Submitted Version","oa":1,"department":[{"_id":"JiFr"},{"_id":"GradSch"},{"_id":"EvBe"},{"_id":"EM-Fac"}],"quality_controlled":"1","language":[{"iso":"eng"}],"issue":"7927","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"09","citation":{"apa":"Friml, J., Gallei, M. C., Gelová, Z., Johnson, A. J., Mazur, E., Monzer, A., … Rakusová, H. (2022). ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>","ista":"Friml J, Gallei MC, Gelová Z, Johnson AJ, Mazur E, Monzer A, Rodriguez Solovey L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones P, Hrtyan M, Kaufmann W, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K, Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H. 2022. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. Nature. 609(7927), 575–581.","ama":"Friml J, Gallei MC, Gelová Z, et al. ABP1–TMK auxin perception for global phosphorylation and auxin canalization. <i>Nature</i>. 2022;609(7927):575-581. doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>","chicago":"Friml, Jiří, Michelle C Gallei, Zuzana Gelová, Alexander J Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez Solovey, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41586-022-05187-x\">https://doi.org/10.1038/s41586-022-05187-x</a>.","short":"J. Friml, M.C. Gallei, Z. Gelová, A.J. Johnson, E. Mazur, A. Monzer, L. Rodriguez Solovey, M. Roosjen, I. Verstraeten, B.D. Živanović, M. Zou, L. Fiedler, C. Giannini, P. Grones, M. Hrtyan, W. Kaufmann, A. Kuhn, M. Narasimhan, M. Randuch, N. Rýdza, K. Takahashi, S. Tan, A. Teplova, T. Kinoshita, D. Weijers, H. Rakusová, Nature 609 (2022) 575–581.","ieee":"J. Friml <i>et al.</i>, “ABP1–TMK auxin perception for global phosphorylation and auxin canalization,” <i>Nature</i>, vol. 609, no. 7927. Springer Nature, pp. 575–581, 2022.","mla":"Friml, Jiří, et al. “ABP1–TMK Auxin Perception for Global Phosphorylation and Auxin Canalization.” <i>Nature</i>, vol. 609, no. 7927, Springer Nature, 2022, pp. 575–81, doi:<a href=\"https://doi.org/10.1038/s41586-022-05187-x\">10.1038/s41586-022-05187-x</a>."},"scopus_import":"1","acknowledgement":"We acknowledge K. Kubiasová for excellent technical assistance, J. Neuhold, A. Lehner and A. Sedivy for technical assistance with protein production and purification at Vienna Biocenter Core Facilities; Creoptix for performing GCI; and the Bioimaging, Electron Microscopy and Life Science Facilities at ISTA, the Plant Sciences Core Facility of CEITEC Masaryk University, the Core Facility CELLIM (MEYS CR, LM2018129 Czech-BioImaging) and J. Sprakel for their assistance. J.F. is grateful to R. Napier for many insightful suggestions and support. We thank all past and present members of the Friml group for their support and for other contributions to this effort to clarify the controversial role of ABP1 over the past seven years. The project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 742985 to J.F. and 833867 to D.W.); the Austrian Science Fund (FWF; P29988 to J.F.); the Netherlands Organization for Scientific Research (NWO; VICI grant 865.14.001 to D.W. and VENI grant VI.Veni.212.003 to A.K.); the Ministry of Education, Science and Technological Development of the Republic of Serbia (contract no. 451-03-68/2022-14/200053 to B.D.Ž.); and the MEXT/JSPS KAKENHI to K.T. (20K06685) and T.K. (20H05687 and 20H05910).","_id":"12291","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"19395"},{"id":"20364","relation":"dissertation_contains","status":"public"}]},"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"date_created":"2023-01-16T10:04:48Z","doi":"10.1038/s41586-022-05187-x","has_accepted_license":"1","intvolume":"       609","isi":1,"author":[{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","last_name":"Gallei"},{"last_name":"Gelová","first_name":"Zuzana","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","full_name":"Gelová, Zuzana","orcid":"0000-0003-4783-1752"},{"first_name":"Alexander J","last_name":"Johnson","orcid":"0000-0002-2739-8843","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Mazur, Ewa","last_name":"Mazur","first_name":"Ewa"},{"first_name":"Aline","last_name":"Monzer","full_name":"Monzer, Aline","id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","full_name":"Rodriguez Solovey, Lesia","orcid":"0000-0002-7244-7237","last_name":"Rodriguez Solovey","first_name":"Lesia"},{"full_name":"Roosjen, Mark","last_name":"Roosjen","first_name":"Mark"},{"orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","first_name":"Inge","last_name":"Verstraeten"},{"first_name":"Branka D.","last_name":"Živanović","full_name":"Živanović, Branka D."},{"id":"5c243f41-03f3-11ec-841c-96faf48a7ef9","full_name":"Zou, Minxia","last_name":"Zou","first_name":"Minxia"},{"first_name":"Lukas","last_name":"Fiedler","full_name":"Fiedler, Lukas","id":"7c417475-8972-11ed-ae7b-8b674ca26986"},{"last_name":"Giannini","first_name":"Caterina","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","full_name":"Giannini, Caterina"},{"full_name":"Grones, Peter","first_name":"Peter","last_name":"Grones"},{"id":"45A71A74-F248-11E8-B48F-1D18A9856A87","full_name":"Hrtyan, Mónika","last_name":"Hrtyan","first_name":"Mónika"},{"orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","last_name":"Kaufmann"},{"last_name":"Kuhn","first_name":"Andre","full_name":"Kuhn, Andre"},{"first_name":"Madhumitha","last_name":"Narasimhan","full_name":"Narasimhan, Madhumitha","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8600-0671"},{"last_name":"Randuch","first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","full_name":"Randuch, Marek"},{"last_name":"Rýdza","first_name":"Nikola","full_name":"Rýdza, Nikola"},{"full_name":"Takahashi, Koji","first_name":"Koji","last_name":"Takahashi"},{"last_name":"Tan","first_name":"Shutang","orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang"},{"id":"e3736151-106c-11ec-b916-c2558e2762c6","full_name":"Teplova, Anastasiia","last_name":"Teplova","first_name":"Anastasiia"},{"last_name":"Kinoshita","first_name":"Toshinori","full_name":"Kinoshita, Toshinori"},{"first_name":"Dolf","last_name":"Weijers","full_name":"Weijers, Dolf"},{"full_name":"Rakusová, Hana","last_name":"Rakusová","first_name":"Hana"}],"corr_author":"1","ddc":["580"],"date_published":"2022-09-15T00:00:00Z","file":[{"creator":"amally","file_size":79774945,"date_updated":"2023-11-02T17:12:37Z","content_type":"application/pdf","date_created":"2023-11-02T17:12:37Z","relation":"main_file","checksum":"a6055c606aefb900bf62ae3e7d15f921","access_level":"open_access","success":1,"file_name":"Friml Nature 2022_merged.pdf","file_id":"14483"}],"year":"2022","day":"15","date_updated":"2026-04-07T11:52:15Z","article_type":"original","publisher":"Springer Nature","pmid":1,"publication_status":"published","status":"public"},{"volume":3,"publication":"Plant Communications","abstract":[{"text":"In plants, the antagonism between growth and defense is hardwired by hormonal signaling. The perception of pathogen-associated molecular patterns (PAMPs) from invading microorganisms inhibits auxin signaling and plant growth. Conversely, pathogens manipulate auxin signaling to promote disease, but how this hormone inhibits immunity is not fully understood. Ustilago maydis is a maize pathogen that induces auxin signaling in its host. We characterized a U. maydis effector protein, Naked1 (Nkd1), that is translocated into the host nucleus. Through its native ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif, Nkd1 binds to the transcriptional co-repressors TOPLESS/TOPLESS-related (TPL/TPRs) and prevents the recruitment of a transcriptional repressor involved in hormonal signaling, leading to the de-repression of auxin and jasmonate signaling and thereby promoting susceptibility to (hemi)biotrophic pathogens. A moderate upregulation of auxin signaling inhibits the PAMP-triggered reactive oxygen species (ROS) burst, an early defense response. Thus, our findings establish a clear mechanism for auxin-induced pathogen susceptibility. Engineered Nkd1 variants with increased expression or increased EAR-mediated TPL/TPR binding trigger typical salicylic-acid-mediated defense reactions, leading to pathogen resistance. This implies that moderate binding of Nkd1 to TPL is a result of a balancing evolutionary selection process to enable TPL manipulation while avoiding host recognition.","lang":"eng"}],"file_date_updated":"2024-08-05T10:26:29Z","title":"TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1","article_number":"100269","article_processing_charge":"Yes","external_id":{"pmid":["35529945"]},"acknowledgement":"The research leading to these results received funding from the European Research Council under the European Union Seventh Framework Programme ERC-2013-STG grant agreement \r\n335691; the Austrian Science Fund (FWF) P27818-B22,I 3033-B22; the Austrian Academy of Sciences (OEAW); and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy - EXC 2070-390732324.\r\nWe would like to thank the GMI/IMBA/IMP core facilities for excellent technical support, especially the BioOptics and Molecular Biology Services. We thank the Plant Sciences and Next Generation Sequencing Facilities at the Vienna BioCenter Core Facilities GmbH (VBCF). We are grateful to the Jirí Friml and Jürgen Kleine-Vehn laboratories for providing useful A. thaliana lines. We thank Mathias Madalinski for peptide synthesis and Dr. J. Matthew Watson for proofreading and valuable feedback on the manuscript. The authors declare no competing interests.","_id":"17068","publication_identifier":{"issn":["2590-3462"]},"date_created":"2024-05-29T06:10:22Z","doi":"10.1016/j.xplc.2021.100269","type":"journal_article","month":"03","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ama":"Navarrete F, Gallei MC, Kornienko AE, et al. TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1. <i>Plant Communications</i>. 2022;3(2). doi:<a href=\"https://doi.org/10.1016/j.xplc.2021.100269\">10.1016/j.xplc.2021.100269</a>","chicago":"Navarrete, Fernando, Michelle C Gallei, Aleksandra E. Kornienko, Indira Saado, Mamoona Khan, Khong-Sam Chia, Martin A. Darino, Janos Bindics, and Armin Djamei. “TOPLESS Promotes Plant Immunity by Repressing Auxin Signaling and Is Targeted by the Fungal Effector Naked1.” <i>Plant Communications</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.xplc.2021.100269\">https://doi.org/10.1016/j.xplc.2021.100269</a>.","short":"F. Navarrete, M.C. Gallei, A.E. Kornienko, I. Saado, M. Khan, K.-S. Chia, M.A. Darino, J. Bindics, A. Djamei, Plant Communications 3 (2022).","ieee":"F. Navarrete <i>et al.</i>, “TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1,” <i>Plant Communications</i>, vol. 3, no. 2. Elsevier, 2022.","mla":"Navarrete, Fernando, et al. “TOPLESS Promotes Plant Immunity by Repressing Auxin Signaling and Is Targeted by the Fungal Effector Naked1.” <i>Plant Communications</i>, vol. 3, no. 2, 100269, Elsevier, 2022, doi:<a href=\"https://doi.org/10.1016/j.xplc.2021.100269\">10.1016/j.xplc.2021.100269</a>.","apa":"Navarrete, F., Gallei, M. C., Kornienko, A. E., Saado, I., Khan, M., Chia, K.-S., … Djamei, A. (2022). TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1. <i>Plant Communications</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.xplc.2021.100269\">https://doi.org/10.1016/j.xplc.2021.100269</a>","ista":"Navarrete F, Gallei MC, Kornienko AE, Saado I, Khan M, Chia K-S, Darino MA, Bindics J, Djamei A. 2022. TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1. Plant Communications. 3(2), 100269."},"scopus_import":"1","language":[{"iso":"eng"}],"quality_controlled":"1","issue":"2","oa":1,"oa_version":"Published Version","department":[{"_id":"JiFr"}],"ddc":["580"],"date_published":"2022-03-14T00:00:00Z","author":[{"last_name":"Navarrete","first_name":"Fernando","full_name":"Navarrete, Fernando"},{"first_name":"Michelle C","last_name":"Gallei","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kornienko","first_name":"Aleksandra E.","full_name":"Kornienko, Aleksandra E."},{"full_name":"Saado, Indira","last_name":"Saado","first_name":"Indira"},{"full_name":"Khan, Mamoona","last_name":"Khan","first_name":"Mamoona"},{"full_name":"Chia, Khong-Sam","last_name":"Chia","first_name":"Khong-Sam"},{"full_name":"Darino, Martin A.","first_name":"Martin A.","last_name":"Darino"},{"first_name":"Janos","last_name":"Bindics","full_name":"Bindics, Janos"},{"full_name":"Djamei, Armin","first_name":"Armin","last_name":"Djamei"}],"intvolume":"         3","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"has_accepted_license":"1","pmid":1,"publication_status":"published","status":"public","date_updated":"2024-08-05T10:27:03Z","publisher":"Elsevier","article_type":"original","year":"2022","day":"14","file":[{"success":1,"file_name":"2022_PlantComm_Navarrete.pdf","file_id":"17393","creator":"dernst","content_type":"application/pdf","date_updated":"2024-08-05T10:26:29Z","file_size":3216686,"date_created":"2024-08-05T10:26:29Z","checksum":"1eeb6ee65419e4aa34627fea6857f343","relation":"main_file","access_level":"open_access"}]},{"project":[{"_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants"},{"name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root","grant_number":"25351","_id":"26B4D67E-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"No","external_id":{"pmid":["34848141"],"isi":["000793707900005"]},"page":"440-449","title":"Bending to auxin: Fast acid growth for tropisms","file_date_updated":"2023-11-02T17:00:03Z","volume":27,"publication":"Trends in Plant Science","abstract":[{"lang":"eng","text":"The phytohormone auxin is the major growth regulator governing tropic responses including gravitropism. Auxin build-up at the lower side of stimulated shoots promotes cell expansion, whereas in roots it inhibits growth, leading to upward shoot bending and downward root bending, respectively. Yet it remains an enigma how the same signal can trigger such opposite cellular responses. In this review, we discuss several recent unexpected insights into the mechanisms underlying auxin regulation of growth, challenging several existing models. We focus on the divergent mechanisms of apoplastic pH regulation in shoots and roots revisiting the classical Acid Growth Theory and discuss coordinated involvement of multiple auxin signaling pathways. From this emerges a more comprehensive, updated picture how auxin regulates growth."}],"department":[{"_id":"JiFr"}],"oa_version":"Submitted Version","oa":1,"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"5","citation":{"apa":"Li, L., Gallei, M. C., &#38; Friml, J. (2022). Bending to auxin: Fast acid growth for tropisms. <i>Trends in Plant Science</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">https://doi.org/10.1016/j.tplants.2021.11.006</a>","ista":"Li L, Gallei MC, Friml J. 2022. Bending to auxin: Fast acid growth for tropisms. Trends in Plant Science. 27(5), 440–449.","chicago":"Li, Lanxin, Michelle C Gallei, and Jiří Friml. “Bending to Auxin: Fast Acid Growth for Tropisms.” <i>Trends in Plant Science</i>. Cell Press, 2022. <a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">https://doi.org/10.1016/j.tplants.2021.11.006</a>.","ama":"Li L, Gallei MC, Friml J. Bending to auxin: Fast acid growth for tropisms. <i>Trends in Plant Science</i>. 2022;27(5):440-449. doi:<a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">10.1016/j.tplants.2021.11.006</a>","mla":"Li, Lanxin, et al. “Bending to Auxin: Fast Acid Growth for Tropisms.” <i>Trends in Plant Science</i>, vol. 27, no. 5, Cell Press, 2022, pp. 440–49, doi:<a href=\"https://doi.org/10.1016/j.tplants.2021.11.006\">10.1016/j.tplants.2021.11.006</a>.","ieee":"L. Li, M. C. Gallei, and J. Friml, “Bending to auxin: Fast acid growth for tropisms,” <i>Trends in Plant Science</i>, vol. 27, no. 5. Cell Press, pp. 440–449, 2022.","short":"L. Li, M.C. Gallei, J. Friml, Trends in Plant Science 27 (2022) 440–449."},"scopus_import":"1","type":"journal_article","month":"05","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["1360-1385"]},"_id":"10411","related_material":{"record":[{"id":"11626","status":"public","relation":"dissertation_contains"}]},"date_created":"2021-12-05T23:01:43Z","doi":"10.1016/j.tplants.2021.11.006","acknowledgement":"The authors thank Alexandra Mally for editing the text. This work was supported by the Austrian Science Fund (FWF) I 3630-B25 to Jiří Friml and the DOC Fellowship of the Austrian Academy of Sciences to Lanxin Li. All figures were created with BioRender.com.","has_accepted_license":"1","isi":1,"intvolume":"        27","author":[{"last_name":"Li","first_name":"Lanxin","orcid":"0000-0002-5607-272X","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","full_name":"Li, Lanxin"},{"full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368","first_name":"Michelle C","last_name":"Gallei"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml"}],"ddc":["580"],"date_published":"2022-05-01T00:00:00Z","corr_author":"1","file":[{"file_id":"14480","file_name":"Li Plants 2021_accepted.pdf","success":1,"access_level":"open_access","date_created":"2023-11-02T17:00:03Z","checksum":"3d94980ee1ff6bec100dd813f6a921a6","relation":"main_file","file_size":805779,"date_updated":"2023-11-02T17:00:03Z","content_type":"application/pdf","creator":"amally"}],"day":"01","year":"2022","article_type":"original","publisher":"Cell Press","date_updated":"2026-04-07T14:18:57Z","pmid":1,"publication_status":"published","status":"public"},{"citation":{"ista":"Navarrete F, Grujic N, Stirnberg A, Saado I, Aleksza D, Gallei MC, Adi H, Alcântara A, Khan M, Bindics J, Trujillo M, Djamei A. 2021. The Pleiades are a cluster of fungal effectors that inhibit host defenses. PLOS Pathogens. 17(6), e1009641.","apa":"Navarrete, F., Grujic, N., Stirnberg, A., Saado, I., Aleksza, D., Gallei, M. C., … Djamei, A. (2021). The Pleiades are a cluster of fungal effectors that inhibit host defenses. <i>PLOS Pathogens</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.ppat.1009641\">https://doi.org/10.1371/journal.ppat.1009641</a>","short":"F. Navarrete, N. Grujic, A. Stirnberg, I. Saado, D. Aleksza, M.C. Gallei, H. Adi, A. Alcântara, M. Khan, J. Bindics, M. Trujillo, A. Djamei, PLOS Pathogens 17 (2021).","ieee":"F. Navarrete <i>et al.</i>, “The Pleiades are a cluster of fungal effectors that inhibit host defenses,” <i>PLOS Pathogens</i>, vol. 17, no. 6. Public Library of Science, 2021.","mla":"Navarrete, Fernando, et al. “The Pleiades Are a Cluster of Fungal Effectors That Inhibit Host Defenses.” <i>PLOS Pathogens</i>, vol. 17, no. 6, e1009641, Public Library of Science, 2021, doi:<a href=\"https://doi.org/10.1371/journal.ppat.1009641\">10.1371/journal.ppat.1009641</a>.","ama":"Navarrete F, Grujic N, Stirnberg A, et al. The Pleiades are a cluster of fungal effectors that inhibit host defenses. <i>PLOS Pathogens</i>. 2021;17(6). doi:<a href=\"https://doi.org/10.1371/journal.ppat.1009641\">10.1371/journal.ppat.1009641</a>","chicago":"Navarrete, Fernando, Nenad Grujic, Alexandra Stirnberg, Indira Saado, David Aleksza, Michelle C Gallei, Hazem Adi, et al. “The Pleiades Are a Cluster of Fungal Effectors That Inhibit Host Defenses.” <i>PLOS Pathogens</i>. Public Library of Science, 2021. <a href=\"https://doi.org/10.1371/journal.ppat.1009641\">https://doi.org/10.1371/journal.ppat.1009641</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"06","type":"journal_article","date_created":"2024-04-03T08:00:34Z","doi":"10.1371/journal.ppat.1009641","_id":"15276","publication_identifier":{"issn":["1553-7374"]},"department":[{"_id":"JiFr"}],"oa":1,"oa_version":"Published Version","issue":"6","quality_controlled":"1","language":[{"iso":"eng"}],"title":"The Pleiades are a cluster of fungal effectors that inhibit host defenses","article_number":"e1009641","file_date_updated":"2024-04-09T10:24:43Z","publication":"PLOS Pathogens","abstract":[{"lang":"eng","text":"Biotrophic plant pathogens secrete effector proteins to manipulate the host physiology. Effectors suppress defenses and induce an environment favorable to disease development. Sequence-based prediction of effector function is impeded by their rapid evolution rate. In the maize pathogen <jats:italic>Ustilago maydis</jats:italic>, effector-coding genes frequently organize in clusters. Here we describe the functional characterization of the <jats:italic>pleiades</jats:italic>, a cluster of ten effector genes, by analyzing the micro- and macroscopic phenotype of the cluster deletion and expressing these proteins <jats:italic>in planta</jats:italic>. Deletion of the <jats:italic>pleiades</jats:italic> leads to strongly impaired virulence and accumulation of reactive oxygen species (ROS) in infected tissue. Eight of the Pleiades suppress the production of ROS upon perception of pathogen associated molecular patterns (PAMPs). Although functionally redundant, the Pleiades target different host components. The paralogs Taygeta1 and Merope1 suppress ROS production in either the cytoplasm or nucleus, respectively. Merope1 targets and promotes the auto-ubiquitination activity of RFI2, a conserved family of E3 ligases that regulates the production of PAMP-triggered ROS burst in plants."}],"volume":17,"external_id":{"pmid":["34166468"]},"article_processing_charge":"Yes","publisher":"Public Library of Science","article_type":"original","date_updated":"2024-04-09T10:26:12Z","status":"public","publication_status":"published","pmid":1,"file":[{"relation":"main_file","date_created":"2024-04-09T10:24:43Z","checksum":"ab8428291a0c14607c4ea5656c029cff","access_level":"open_access","creator":"dernst","content_type":"application/pdf","date_updated":"2024-04-09T10:24:43Z","file_size":2616563,"file_name":"2021_PlosPathogens_Navarrete.pdf","file_id":"15305","success":1}],"day":"24","year":"2021","author":[{"full_name":"Navarrete, Fernando","first_name":"Fernando","last_name":"Navarrete"},{"first_name":"Nenad","last_name":"Grujic","full_name":"Grujic, Nenad"},{"first_name":"Alexandra","last_name":"Stirnberg","full_name":"Stirnberg, Alexandra"},{"full_name":"Saado, Indira","last_name":"Saado","first_name":"Indira"},{"last_name":"Aleksza","first_name":"David","full_name":"Aleksza, David"},{"last_name":"Gallei","first_name":"Michelle C","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C"},{"last_name":"Adi","first_name":"Hazem","full_name":"Adi, Hazem"},{"first_name":"André","last_name":"Alcântara","full_name":"Alcântara, André"},{"full_name":"Khan, Mamoona","last_name":"Khan","first_name":"Mamoona"},{"full_name":"Bindics, Janos","last_name":"Bindics","first_name":"Janos"},{"first_name":"Marco","last_name":"Trujillo","full_name":"Trujillo, Marco"},{"first_name":"Armin","last_name":"Djamei","full_name":"Djamei, Armin"}],"date_published":"2021-06-24T00:00:00Z","ddc":["580"],"keyword":["Virology","Genetics","Molecular Biology","Immunology","Microbiology","Parasitology"],"has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        17"},{"status":"public","pmid":1,"publication_status":"published","date_updated":"2026-06-27T22:30:45Z","publisher":"Elsevier","article_type":"original","year":"2021","day":"01","file":[{"access_level":"open_access","date_created":"2021-02-04T07:49:25Z","checksum":"a7f2562bdca62d67dfa88e271b62a629","relation":"main_file","content_type":"application/pdf","date_updated":"2021-02-04T07:49:25Z","file_size":12563728,"creator":"dernst","file_id":"9083","file_name":"2021_PlantScience_Gelova.pdf","success":1}],"corr_author":"1","ddc":["580"],"date_published":"2021-02-01T00:00:00Z","author":[{"first_name":"Zuzana","last_name":"Gelová","full_name":"Gelová, Zuzana","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","orcid":"0000-0003-4783-1752"},{"orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","last_name":"Gallei"},{"full_name":"Pernisová, Markéta","last_name":"Pernisová","first_name":"Markéta"},{"last_name":"Brunoud","first_name":"Géraldine","full_name":"Brunoud, Géraldine"},{"first_name":"Xixi","last_name":"Zhang","full_name":"Zhang, Xixi","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","orcid":"0000-0001-7048-4627"},{"first_name":"Matous","last_name":"Glanc","orcid":"0000-0003-0619-7783","full_name":"Glanc, Matous","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2"},{"last_name":"Li","first_name":"Lanxin","orcid":"0000-0002-5607-272X","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","full_name":"Li, Lanxin"},{"last_name":"Michalko","first_name":"Jaroslav","id":"483727CA-F248-11E8-B48F-1D18A9856A87","full_name":"Michalko, Jaroslav"},{"last_name":"Pavlovicova","first_name":"Zlata","full_name":"Pavlovicova, Zlata"},{"orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","first_name":"Inge","last_name":"Verstraeten"},{"last_name":"Han","first_name":"Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","full_name":"Han, Huibin"},{"last_name":"Hajny","first_name":"Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","full_name":"Hajny, Jakub","orcid":"0000-0003-2140-7195"},{"last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert"},{"full_name":"Čovanová, Milada","first_name":"Milada","last_name":"Čovanová"},{"first_name":"Marta","last_name":"Zwiewka","full_name":"Zwiewka, Marta"},{"last_name":"Hörmayer","first_name":"Lukas","orcid":"0000-0001-8295-2926","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","full_name":"Hörmayer, Lukas"},{"last_name":"Fendrych","first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","full_name":"Fendrych, Matyas","orcid":"0000-0002-9767-8699"},{"last_name":"Xu","first_name":"Tongda","full_name":"Xu, Tongda"},{"last_name":"Vernoux","first_name":"Teva","full_name":"Vernoux, Teva"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"}],"intvolume":"       303","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"keyword":["Agronomy and Crop Science","Plant Science","Genetics","General Medicine"],"has_accepted_license":"1","acknowledgement":"We would like to acknowledge Bioimaging and Life Science Facilities at IST Austria for continuous support and also the Plant Sciences Core Facility of CEITEC Masaryk University for their support with obtaining a part of the scientific data. We gratefully acknowledge Lindy Abas for help with ABP1::GFP-ABP1 construct design. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program [grant agreement no. 742985] and Austrian Science Fund (FWF) [I 3630-B25] to J.F.; DOC Fellowship of the Austrian Academy of Sciences to L.L.; the European Structural and Investment Funds, Operational Programme Research, Development and Education - Project „MSCAfellow@MUNI“ [CZ.02.2.69/0.0/0.0/17_050/0008496] to M.P.. This project was also supported by the Czech Science Foundation [GA 20-20860Y] to M.Z and MEYS CR [project no.CZ.02.1.01/0.0/0.0/16_019/0000738] to M. Č.","doi":"10.1016/j.plantsci.2020.110750","date_created":"2020-12-09T14:48:28Z","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"11626"},{"status":"public","relation":"dissertation_contains","id":"10083"}]},"_id":"8931","publication_identifier":{"issn":["0168-9452"]},"month":"02","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","type":"journal_article","scopus_import":"1","citation":{"ieee":"Z. Gelová <i>et al.</i>, “Developmental roles of auxin binding protein 1 in Arabidopsis thaliana,” <i>Plant Science</i>, vol. 303. Elsevier, 2021.","mla":"Gelová, Zuzana, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis Thaliana.” <i>Plant Science</i>, vol. 303, 110750, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">10.1016/j.plantsci.2020.110750</a>.","short":"Z. Gelová, M.C. Gallei, M. Pernisová, G. Brunoud, X. Zhang, M. Glanc, L. Li, J. Michalko, Z. Pavlovicova, I. Verstraeten, H. Han, J. Hajny, R. Hauschild, M. Čovanová, M. Zwiewka, L. Hörmayer, M. Fendrych, T. Xu, T. Vernoux, J. Friml, Plant Science 303 (2021).","chicago":"Gelová, Zuzana, Michelle C Gallei, Markéta Pernisová, Géraldine Brunoud, Xixi Zhang, Matous Glanc, Lanxin Li, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis Thaliana.” <i>Plant Science</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">https://doi.org/10.1016/j.plantsci.2020.110750</a>.","ama":"Gelová Z, Gallei MC, Pernisová M, et al. Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. <i>Plant Science</i>. 2021;303. doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">10.1016/j.plantsci.2020.110750</a>","ista":"Gelová Z, Gallei MC, Pernisová M, Brunoud G, Zhang X, Glanc M, Li L, Michalko J, Pavlovicova Z, Verstraeten I, Han H, Hajny J, Hauschild R, Čovanová M, Zwiewka M, Hörmayer L, Fendrych M, Xu T, Vernoux T, Friml J. 2021. Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. Plant Science. 303, 110750.","apa":"Gelová, Z., Gallei, M. C., Pernisová, M., Brunoud, G., Zhang, X., Glanc, M., … Friml, J. (2021). Developmental roles of auxin binding protein 1 in Arabidopsis thaliana. <i>Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110750\">https://doi.org/10.1016/j.plantsci.2020.110750</a>"},"language":[{"iso":"eng"}],"quality_controlled":"1","oa":1,"oa_version":"Published Version","department":[{"_id":"JiFr"},{"_id":"Bio"}],"abstract":[{"lang":"eng","text":"Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of auxin effects, in particular those associated with rapid responses. A possible candidate for a component of additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1), whose function in planta remains unclear.\r\nHere we combined expression analysis with gain- and loss-of-function approaches to analyze the role of ABP1 in plant development. ABP1 shows a broad expression largely overlapping with, but not regulated by, transcriptional auxin response activity. Furthermore, ABP1 activity is not essential for the transcriptional auxin signaling. Genetic in planta analysis revealed that abp1 loss-of-function mutants show largely normal development with minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show a broad range of growth and developmental defects, including root and hypocotyl growth and bending, lateral root and leaf development, bolting, as well as response to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular aggregation.\r\nThe gain-of-function analysis suggests a broad, but still mechanistically unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function mutants by a functional redundancy."}],"publication":"Plant Science","ec_funded":1,"volume":303,"file_date_updated":"2021-02-04T07:49:25Z","title":"Developmental roles of auxin binding protein 1 in Arabidopsis thaliana","article_number":"110750","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"article_processing_charge":"Yes (via OA deal)","external_id":{"isi":["000614154500001"],"pmid":["33487339"]},"project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants"},{"_id":"26B4D67E-B435-11E9-9278-68D0E5697425","grant_number":"25351","name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root"}]},{"author":[{"last_name":"Narasimhan","first_name":"Madhumitha","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","full_name":"Narasimhan, Madhumitha","orcid":"0000-0002-8600-0671"},{"first_name":"Michelle C","last_name":"Gallei","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368"},{"orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","last_name":"Tan","first_name":"Shutang"},{"first_name":"Alexander J","last_name":"Johnson","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843"},{"last_name":"Verstraeten","first_name":"Inge","orcid":"0000-0001-7241-2328","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","full_name":"Verstraeten, Inge"},{"last_name":"Li","first_name":"Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","full_name":"Li, Lanxin","orcid":"0000-0002-5607-272X"},{"first_name":"Lesia","last_name":"Rodriguez Solovey","orcid":"0000-0002-7244-7237","full_name":"Rodriguez Solovey, Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Han, Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","first_name":"Huibin","last_name":"Han"},{"full_name":"Himschoot, E","first_name":"E","last_name":"Himschoot"},{"full_name":"Wang, R","first_name":"R","last_name":"Wang"},{"last_name":"Vanneste","first_name":"S","full_name":"Vanneste, S"},{"first_name":"J","last_name":"Sánchez-Simarro","full_name":"Sánchez-Simarro, J"},{"full_name":"Aniento, F","last_name":"Aniento","first_name":"F"},{"full_name":"Adamowski, Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6463-5257","first_name":"Maciek","last_name":"Adamowski"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"date_published":"2021-06-01T00:00:00Z","ddc":["580"],"corr_author":"1","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"intvolume":"       186","article_type":"original","publisher":"Oxford University Press","date_updated":"2026-06-27T22:30:45Z","status":"public","pmid":1,"publication_status":"published","file":[{"access_level":"open_access","checksum":"532bb9469d3b665907f06df8c383eade","relation":"main_file","date_created":"2021-11-11T15:07:51Z","date_updated":"2021-11-11T15:07:51Z","content_type":"application/pdf","file_size":2289127,"creator":"cziletti","file_id":"10273","file_name":"2021_PlantPhysio_Narasimhan.pdf","success":1}],"day":"01","year":"2021","title":"Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking","file_date_updated":"2021-11-11T15:07:51Z","abstract":[{"text":"The phytohormone auxin and its directional transport through tissues are intensively studied. However, a mechanistic understanding of auxin-mediated feedback on endocytosis and polar distribution of PIN auxin transporters remains limited due to contradictory observations and interpretations. Here, we used state-of-the-art methods to reexamine the\r\nauxin effects on PIN endocytic trafficking. We used high auxin concentrations or longer treatments versus lower concentrations and shorter treatments of natural (IAA) and synthetic (NAA) auxins to distinguish between specific and nonspecific effects. Longer treatments of both auxins interfere with Brefeldin A-mediated intracellular PIN2 accumulation and also with general aggregation of endomembrane compartments. NAA treatment decreased the internalization of the endocytic tracer dye, FM4-64; however, NAA treatment also affected the number, distribution, and compartment identity of the early endosome/trans-Golgi network (EE/TGN), rendering the FM4-64 endocytic assays at high NAA concentrations unreliable. To circumvent these nonspecific effects of NAA and IAA affecting the endomembrane system, we opted for alternative approaches visualizing the endocytic events directly at the plasma membrane (PM). Using Total Internal Reflection Fluorescence (TIRF) microscopy, we saw no significant effects of IAA or NAA treatments on the incidence and dynamics of clathrin foci, implying that these treatments do not affect the overall endocytosis rate. However, both NAA and IAA at low concentrations rapidly and specifically promoted endocytosis of photo-converted PIN2 from the PM. These analyses identify a specific effect of NAA and IAA on PIN2 endocytosis, thus contributing to its\r\npolarity maintenance and furthermore illustrate that high auxin levels have nonspecific effects on trafficking and endomembrane compartments. ","lang":"eng"}],"publication":"Plant Physiology","ec_funded":1,"volume":186,"project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF"}],"page":"1122–1142","external_id":{"isi":["000671555900031"],"pmid":["33734402"]},"article_processing_charge":"Yes (in subscription journal)","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"scopus_import":"1","citation":{"mla":"Narasimhan, Madhumitha, et al. “Systematic Analysis of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” <i>Plant Physiology</i>, vol. 186, no. 2, Oxford University Press, 2021, pp. 1122–1142, doi:<a href=\"https://doi.org/10.1093/plphys/kiab134\">10.1093/plphys/kiab134</a>.","ieee":"M. Narasimhan <i>et al.</i>, “Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking,” <i>Plant Physiology</i>, vol. 186, no. 2. Oxford University Press, pp. 1122–1142, 2021.","short":"M. Narasimhan, M.C. Gallei, S. Tan, A.J. Johnson, I. Verstraeten, L. Li, L. Rodriguez Solovey, H. Han, E. Himschoot, R. Wang, S. Vanneste, J. Sánchez-Simarro, F. Aniento, M. Adamowski, J. Friml, Plant Physiology 186 (2021) 1122–1142.","chicago":"Narasimhan, Madhumitha, Michelle C Gallei, Shutang Tan, Alexander J Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez Solovey, et al. “Systematic Analysis of Specific and Nonspecific Auxin Effects on Endocytosis and Trafficking.” <i>Plant Physiology</i>. Oxford University Press, 2021. <a href=\"https://doi.org/10.1093/plphys/kiab134\">https://doi.org/10.1093/plphys/kiab134</a>.","ama":"Narasimhan M, Gallei MC, Tan S, et al. Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. <i>Plant Physiology</i>. 2021;186(2):1122–1142. doi:<a href=\"https://doi.org/10.1093/plphys/kiab134\">10.1093/plphys/kiab134</a>","ista":"Narasimhan M, Gallei MC, Tan S, Johnson AJ, Verstraeten I, Li L, Rodriguez Solovey L, Han H, Himschoot E, Wang R, Vanneste S, Sánchez-Simarro J, Aniento F, Adamowski M, Friml J. 2021. Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. Plant Physiology. 186(2), 1122–1142.","apa":"Narasimhan, M., Gallei, M. C., Tan, S., Johnson, A. J., Verstraeten, I., Li, L., … Friml, J. (2021). Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. <i>Plant Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plphys/kiab134\">https://doi.org/10.1093/plphys/kiab134</a>"},"month":"06","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","type":"journal_article","doi":"10.1093/plphys/kiab134","date_created":"2021-03-26T12:08:38Z","related_material":{"record":[{"id":"11626","relation":"dissertation_contains","status":"public"},{"relation":"dissertation_contains","status":"public","id":"10083"}],"link":[{"url":"https://doi.org/10.1093/plphys/kiab380","relation":"erratum"}]},"_id":"9287","publication_identifier":{"eissn":["1532-2548"],"issn":["0032-0889"]},"acknowledgement":"We thank Ivan Kulik for developing the Chip’n’Dale apparatus with Lanxin Li; the IST machine shop and the Bioimaging facility for their excellent support; Matouš Glanc and Matyáš Fendrych for their valuable discussions and help; Barbara Casillas-Perez for her help with statistics. This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No 742985). A.J. is supported by funding from the Austrian Science Fund (FWF): I3630B25 to J.F. ","department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1,"issue":"2","language":[{"iso":"eng"}],"quality_controlled":"1"},{"oa":1,"oa_version":"Published Version","department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"1","type":"journal_article","month":"07","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"ista":"Zhang J, Mazur E, Balla J, Gallei MC, Kalousek P, Medveďová Z, Li Y, Wang Y, Prat T, Vasileva MK, Reinöhl V, Procházka S, Halouzka R, Tarkowski P, Luschnig C, Brewer P, Friml J. 2020. Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. Nature Communications. 11(1), 3508.","apa":"Zhang, J., Mazur, E., Balla, J., Gallei, M. C., Kalousek, P., Medveďová, Z., … Friml, J. (2020). Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-17252-y\">https://doi.org/10.1038/s41467-020-17252-y</a>","mla":"Zhang, J., et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin Transport Canalization.” <i>Nature Communications</i>, vol. 11, no. 1, Springer Nature, 2020, p. 3508, doi:<a href=\"https://doi.org/10.1038/s41467-020-17252-y\">10.1038/s41467-020-17252-y</a>.","ieee":"J. Zhang <i>et al.</i>, “Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization,” <i>Nature Communications</i>, vol. 11, no. 1. Springer Nature, p. 3508, 2020.","short":"J. Zhang, E. Mazur, J. Balla, M.C. Gallei, P. Kalousek, Z. Medveďová, Y. Li, Y. Wang, T. Prat, M.K. Vasileva, V. Reinöhl, S. Procházka, R. Halouzka, P. Tarkowski, C. Luschnig, P. Brewer, J. Friml, Nature Communications 11 (2020) 3508.","chicago":"Zhang, J, E Mazur, J Balla, Michelle C Gallei, P Kalousek, Z Medveďová, Y Li, et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin Transport Canalization.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-17252-y\">https://doi.org/10.1038/s41467-020-17252-y</a>.","ama":"Zhang J, Mazur E, Balla J, et al. Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization. <i>Nature Communications</i>. 2020;11(1):3508. doi:<a href=\"https://doi.org/10.1038/s41467-020-17252-y\">10.1038/s41467-020-17252-y</a>"},"scopus_import":"1","acknowledgement":"We are grateful to David Nelson for providing published materials and extremely helpful comments, and Elizabeth Dun and Christine Beveridge for helpful discussions. The research leading to these results has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (742985). This work was also supported by the Beijing Municipal Natural Science Foundation (5192011), Beijing Outstanding University Discipline Program, the National Natural Science Foundation of China (31370309), CEITEC 2020 (LQ1601) project with financial contribution made by the Ministry of Education, Youth and Sports of the Czech Republic within special support paid from the National Program of Sustainability II funds, Australian Research Council (FT180100081), and China Postdoctoral Science Foundation (2019M660864).","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"11626"}]},"_id":"8138","publication_identifier":{"issn":["2041-1723"]},"date_created":"2020-07-21T08:58:07Z","doi":"10.1038/s41467-020-17252-y","article_processing_charge":"No","page":"3508","external_id":{"isi":["000550062200004"],"pmid":["32665554"]},"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"}],"title":"Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization","volume":11,"ec_funded":1,"publication":"Nature Communications","abstract":[{"text":"Directional transport of the phytohormone auxin is a versatile, plant-specific mechanism regulating many aspects of plant development. The recently identified plant hormones, strigolactones (SLs), are implicated in many plant traits; among others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters for fine-tuning of growth and developmental responses. Here, we show in pea and Arabidopsis that SLs target processes dependent on the canalization of auxin flow, which involves auxin feedback on PIN subcellular distribution. D14 receptor- and MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels after wounding or from artificial auxin sources, during vasculature de novo formation and regeneration. At the cellular level, SLs interfere with auxin effects on PIN polar targeting, constitutive PIN trafficking as well as clathrin-mediated endocytosis. Our results identify a non-transcriptional mechanism of SL action, uncoupling auxin feedback on PIN polarity and trafficking, thereby regulating vascular tissue formation and regeneration.","lang":"eng"}],"file_date_updated":"2020-07-22T08:32:55Z","file":[{"file_size":1759490,"content_type":"application/pdf","date_updated":"2020-07-22T08:32:55Z","creator":"dernst","access_level":"open_access","relation":"main_file","date_created":"2020-07-22T08:32:55Z","success":1,"file_id":"8148","file_name":"2020_NatureComm_Zhang.pdf"}],"year":"2020","day":"14","date_updated":"2026-04-07T14:18:57Z","article_type":"original","publisher":"Springer Nature","pmid":1,"publication_status":"published","status":"public","has_accepted_license":"1","intvolume":"        11","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"author":[{"full_name":"Zhang, J","first_name":"J","last_name":"Zhang"},{"full_name":"Mazur, E","last_name":"Mazur","first_name":"E"},{"full_name":"Balla, J","first_name":"J","last_name":"Balla"},{"first_name":"Michelle C","last_name":"Gallei","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kalousek, P","last_name":"Kalousek","first_name":"P"},{"full_name":"Medveďová, Z","first_name":"Z","last_name":"Medveďová"},{"last_name":"Li","first_name":"Y","full_name":"Li, Y"},{"first_name":"Y","last_name":"Wang","full_name":"Wang, Y"},{"last_name":"Prat","first_name":"Tomas","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","full_name":"Prat, Tomas"},{"first_name":"Mina K","last_name":"Vasileva","full_name":"Vasileva, Mina K","id":"3407EB18-F248-11E8-B48F-1D18A9856A87"},{"first_name":"V","last_name":"Reinöhl","full_name":"Reinöhl, V"},{"full_name":"Procházka, S","last_name":"Procházka","first_name":"S"},{"first_name":"R","last_name":"Halouzka","full_name":"Halouzka, R"},{"last_name":"Tarkowski","first_name":"P","full_name":"Tarkowski, P"},{"first_name":"C","last_name":"Luschnig","full_name":"Luschnig, C"},{"first_name":"PB","last_name":"Brewer","full_name":"Brewer, PB"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"}],"corr_author":"1","date_published":"2020-07-14T00:00:00Z","ddc":["580"]},{"corr_author":"1","date_published":"2020-02-01T00:00:00Z","author":[{"first_name":"Michelle C","last_name":"Gallei","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368"},{"first_name":"Christian","last_name":"Luschnig","full_name":"Luschnig, Christian"},{"last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří"}],"intvolume":"        53","isi":1,"pmid":1,"publication_status":"published","status":"public","date_updated":"2026-04-07T14:18:57Z","article_type":"original","publisher":"Elsevier","year":"2020","day":"01","volume":53,"ec_funded":1,"publication":"Current Opinion in Plant Biology","abstract":[{"lang":"eng","text":"The phytohormone auxin acts as an amazingly versatile coordinator of plant growth and development. With its morphogen-like properties, auxin controls sites and timing of differentiation and/or growth responses both, in quantitative and qualitative terms. Specificity in the auxin response depends largely on distinct modes of signal transmission, by which individual cells perceive and convert auxin signals into a remarkable diversity of responses. The best understood, or so-called canonical mechanism of auxin perception ultimately results in variable adjustments of the cellular transcriptome, via a short, nuclear signal transduction pathway. Additional findings that accumulated over decades implied that an additional, presumably, cell surface-based auxin perception mechanism mediates very rapid cellular responses and decisively contributes to the cell's overall hormonal response. Recent investigations into both, nuclear and cell surface auxin signalling challenged this assumed partition of roles for different auxin signalling pathways and revealed an unexpected complexity in transcriptional and non-transcriptional cellular responses mediated by auxin."}],"title":"Auxin signalling in growth: Schrödinger's cat out of the bag","article_processing_charge":"No","external_id":{"isi":["000521120600007"],"pmid":["31760231"]},"page":"43-49","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"acknowledgement":"Research in J.F. laboratory is funded by the European Union's Horizon 2020 program (ERC grant agreement n° 742985); C.L. is supported by the Austrian Science Fund (FWF grant P 31493).","_id":"7142","related_material":{"record":[{"id":"11626","status":"public","relation":"dissertation_contains"}]},"publication_identifier":{"eissn":["1879-0356"],"issn":["1369-5266"]},"doi":"10.1016/j.pbi.2019.10.003","date_created":"2019-12-02T12:05:26Z","type":"journal_article","month":"02","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","citation":{"apa":"Gallei, M. C., Luschnig, C., &#38; Friml, J. (2020). Auxin signalling in growth: Schrödinger’s cat out of the bag. <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">https://doi.org/10.1016/j.pbi.2019.10.003</a>","ista":"Gallei MC, Luschnig C, Friml J. 2020. Auxin signalling in growth: Schrödinger’s cat out of the bag. Current Opinion in Plant Biology. 53(2), 43–49.","ama":"Gallei MC, Luschnig C, Friml J. Auxin signalling in growth: Schrödinger’s cat out of the bag. <i>Current Opinion in Plant Biology</i>. 2020;53(2):43-49. doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">10.1016/j.pbi.2019.10.003</a>","chicago":"Gallei, Michelle C, Christian Luschnig, and Jiří Friml. “Auxin Signalling in Growth: Schrödinger’s Cat out of the Bag.” <i>Current Opinion in Plant Biology</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">https://doi.org/10.1016/j.pbi.2019.10.003</a>.","short":"M.C. Gallei, C. Luschnig, J. Friml, Current Opinion in Plant Biology 53 (2020) 43–49.","mla":"Gallei, Michelle C., et al. “Auxin Signalling in Growth: Schrödinger’s Cat out of the Bag.” <i>Current Opinion in Plant Biology</i>, vol. 53, no. 2, Elsevier, 2020, pp. 43–49, doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">10.1016/j.pbi.2019.10.003</a>.","ieee":"M. C. Gallei, C. Luschnig, and J. Friml, “Auxin signalling in growth: Schrödinger’s cat out of the bag,” <i>Current Opinion in Plant Biology</i>, vol. 53, no. 2. Elsevier, pp. 43–49, 2020."},"scopus_import":"1","quality_controlled":"1","language":[{"iso":"eng"}],"issue":"2","oa_version":"None","department":[{"_id":"JiFr"}]},{"type":"journal_article","month":"04","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","citation":{"short":"E. Mazur, M.C. Gallei, M. Adamowski, H. Han, H.S. Robert, J. Friml, Plant Science 293 (2020).","ieee":"E. Mazur, M. C. Gallei, M. Adamowski, H. Han, H. S. Robert, and J. Friml, “Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis,” <i>Plant Science</i>, vol. 293, no. 4. Elsevier, 2020.","mla":"Mazur, Ewa, et al. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” <i>Plant Science</i>, vol. 293, no. 4, 110414, Elsevier, 2020, doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110414\">10.1016/j.plantsci.2020.110414</a>.","ama":"Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. <i>Plant Science</i>. 2020;293(4). doi:<a href=\"https://doi.org/10.1016/j.plantsci.2020.110414\">10.1016/j.plantsci.2020.110414</a>","chicago":"Mazur, Ewa, Michelle C Gallei, Maciek Adamowski, Huibin Han, Hélène S. Robert, and Jiří Friml. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” <i>Plant Science</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110414\">https://doi.org/10.1016/j.plantsci.2020.110414</a>.","ista":"Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. 2020. Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. Plant Science. 293(4), 110414.","apa":"Mazur, E., Gallei, M. C., Adamowski, M., Han, H., Robert, H. S., &#38; Friml, J. (2020). Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis. <i>Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.plantsci.2020.110414\">https://doi.org/10.1016/j.plantsci.2020.110414</a>"},"scopus_import":"1","related_material":{"record":[{"id":"11626","status":"public","relation":"dissertation_contains"}]},"_id":"7465","publication_identifier":{"eissn":["1873-2259"],"issn":["0168-9452"]},"date_created":"2020-02-09T23:00:50Z","doi":"10.1016/j.plantsci.2020.110414","oa":1,"oa_version":"Published Version","department":[{"_id":"JiFr"}],"quality_controlled":"1","language":[{"iso":"eng"}],"issue":"4","article_number":"110414","title":"Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization and vascular tissue formation in Arabidopsis","volume":293,"ec_funded":1,"abstract":[{"text":"The flexible development of plants is characterized by a high capacity for post-embryonic organ formation and tissue regeneration, processes, which require tightly regulated intercellular communication and coordinated tissue (re-)polarization. The phytohormone auxin, the main driver for these processes, is able to establish polarized auxin transport channels, which are characterized by the expression and polar, subcellular localization of the PIN1 auxin transport proteins. These channels are demarcating the position of future vascular strands necessary for organ formation and tissue regeneration. Major progress has been made in the last years to understand how PINs can change their polarity in different contexts and thus guide auxin flow through the plant. However, it still remains elusive how auxin mediates the establishment of auxin conducting channels and the formation of vascular tissue and which cellular processes are involved. By the means of sophisticated regeneration experiments combined with local auxin applications in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics, (ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact actin cytoskeleton required for post-endocytic trafficking are indispensable for auxin channel formation, de novo vascular formation and vascular regeneration after wounding. These observations provide novel insights into cellular mechanism of coordinated tissue polarization during auxin canalization.","lang":"eng"}],"publication":"Plant Science","file_date_updated":"2020-07-14T12:47:59Z","article_processing_charge":"No","external_id":{"isi":["000520609800009"],"pmid":["32081263"]},"project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"}],"date_updated":"2026-04-07T14:18:57Z","article_type":"original","publisher":"Elsevier","pmid":1,"publication_status":"published","status":"public","file":[{"file_name":"2020_PlantScience_Mazur.pdf","file_id":"7471","creator":"dernst","file_size":3499069,"content_type":"application/pdf","date_updated":"2020-07-14T12:47:59Z","checksum":"f7f27c6a8fea985ceb9279be2204461c","date_created":"2020-02-10T08:59:36Z","relation":"main_file","access_level":"open_access"}],"year":"2020","day":"01","author":[{"full_name":"Mazur, Ewa","last_name":"Mazur","first_name":"Ewa"},{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","first_name":"Michelle C"},{"last_name":"Adamowski","first_name":"Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","full_name":"Adamowski, Maciek","orcid":"0000-0001-6463-5257"},{"first_name":"Huibin","last_name":"Han","full_name":"Han, Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Robert, Hélène S.","last_name":"Robert","first_name":"Hélène S."},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"}],"corr_author":"1","date_published":"2020-04-01T00:00:00Z","ddc":["580"],"has_accepted_license":"1","intvolume":"       293","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"}},{"publication_identifier":{"eissn":["1664-462X"]},"_id":"7182","doi":"10.3389/fpls.2019.01437","date_created":"2019-12-15T23:00:43Z","type":"journal_article","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","month":"11","citation":{"apa":"Alcântara, A., Bosch, J., Nazari, F., Hoffmann, G., Gallei, M. C., Uhse, S., … Djamei, A. (2019). Systematic Y2H screening reveals extensive effector-complex formation. <i>Frontiers in Plant Science</i>. Frontiers. <a href=\"https://doi.org/10.3389/fpls.2019.01437\">https://doi.org/10.3389/fpls.2019.01437</a>","ista":"Alcântara A, Bosch J, Nazari F, Hoffmann G, Gallei MC, Uhse S, Darino MA, Olukayode T, Reumann D, Baggaley L, Djamei A. 2019. Systematic Y2H screening reveals extensive effector-complex formation. Frontiers in Plant Science. 10(11), 1437.","chicago":"Alcântara, André, Jason Bosch, Fahimeh Nazari, Gesa Hoffmann, Michelle C Gallei, Simon Uhse, Martin A. Darino, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.” <i>Frontiers in Plant Science</i>. Frontiers, 2019. <a href=\"https://doi.org/10.3389/fpls.2019.01437\">https://doi.org/10.3389/fpls.2019.01437</a>.","ama":"Alcântara A, Bosch J, Nazari F, et al. Systematic Y2H screening reveals extensive effector-complex formation. <i>Frontiers in Plant Science</i>. 2019;10(11). doi:<a href=\"https://doi.org/10.3389/fpls.2019.01437\">10.3389/fpls.2019.01437</a>","mla":"Alcântara, André, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.” <i>Frontiers in Plant Science</i>, vol. 10, no. 11, 1437, Frontiers, 2019, doi:<a href=\"https://doi.org/10.3389/fpls.2019.01437\">10.3389/fpls.2019.01437</a>.","ieee":"A. Alcântara <i>et al.</i>, “Systematic Y2H screening reveals extensive effector-complex formation,” <i>Frontiers in Plant Science</i>, vol. 10, no. 11. Frontiers, 2019.","short":"A. Alcântara, J. Bosch, F. Nazari, G. Hoffmann, M.C. Gallei, S. Uhse, M.A. Darino, T. Olukayode, D. Reumann, L. Baggaley, A. Djamei, Frontiers in Plant Science 10 (2019)."},"scopus_import":"1","language":[{"iso":"eng"}],"quality_controlled":"1","issue":"11","oa_version":"Published Version","oa":1,"department":[{"_id":"JiFr"}],"volume":10,"abstract":[{"lang":"eng","text":"During infection pathogens secrete small molecules, termed effectors, to manipulate and control the interaction with their specific hosts. Both the pathogen and the plant are under high selective pressure to rapidly adapt and co-evolve in what is usually referred to as molecular arms race. Components of the host’s immune system form a network that processes information about molecules with a foreign origin and damage-associated signals, integrating them with developmental and abiotic cues to adapt the plant’s responses. Both in the case of nucleotide-binding leucine-rich repeat receptors and leucine-rich repeat receptor kinases interaction networks have been extensively characterized. However, little is known on whether pathogenic effectors form complexes to overcome plant immunity and promote disease. Ustilago maydis, a biotrophic fungal pathogen that infects maize plants, produces effectors that target hubs in the immune network of the host cell. Here we assess the capability of U. maydis effector candidates to interact with each other, which may play a crucial role during the infection process. Using a systematic yeast-two-hybrid approach and based on a preliminary pooled screen, we selected 63 putative effectors for one-on-one matings with a library of nearly 300 effector candidates. We found that 126 of these effector candidates interacted either with themselves or other predicted effectors. Although the functional relevance of the observed interactions remains elusive, we propose that the observed abundance in complex formation between effectors adds an additional level of complexity to effector research and should be taken into consideration when studying effector evolution and function. Based on this fundamental finding, we suggest various scenarios which could evolutionarily drive the formation and stabilization of an effector interactome."}],"publication":"Frontiers in Plant Science","file_date_updated":"2020-07-14T12:47:52Z","article_number":"1437","title":"Systematic Y2H screening reveals extensive effector-complex formation","article_processing_charge":"No","external_id":{"pmid":["31803201"],"isi":["000499821700001"]},"publication_status":"published","pmid":1,"status":"public","date_updated":"2026-04-16T08:34:31Z","publisher":"Frontiers","article_type":"original","year":"2019","day":"14","file":[{"file_id":"7185","file_name":"2019_FrontiersPlant_Alcantara.pdf","file_size":1532505,"date_updated":"2020-07-14T12:47:52Z","content_type":"application/pdf","creator":"dernst","access_level":"open_access","date_created":"2019-12-16T07:58:43Z","checksum":"995aa838aec2064d93550de82b40bbd1","relation":"main_file"}],"ddc":["580"],"date_published":"2019-11-14T00:00:00Z","author":[{"full_name":"Alcântara, André","first_name":"André","last_name":"Alcântara"},{"first_name":"Jason","last_name":"Bosch","full_name":"Bosch, Jason"},{"first_name":"Fahimeh","last_name":"Nazari","full_name":"Nazari, Fahimeh"},{"first_name":"Gesa","last_name":"Hoffmann","full_name":"Hoffmann, Gesa"},{"full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368","first_name":"Michelle C","last_name":"Gallei"},{"full_name":"Uhse, Simon","last_name":"Uhse","first_name":"Simon"},{"full_name":"Darino, Martin A.","last_name":"Darino","first_name":"Martin A."},{"first_name":"Toluwase","last_name":"Olukayode","full_name":"Olukayode, Toluwase"},{"full_name":"Reumann, Daniel","first_name":"Daniel","last_name":"Reumann"},{"full_name":"Baggaley, Laura","first_name":"Laura","last_name":"Baggaley"},{"full_name":"Djamei, Armin","first_name":"Armin","last_name":"Djamei"}],"intvolume":"        10","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1"},{"title":"Pinstatic acid promotes auxin transport by inhibiting PIN internalization","volume":180,"ec_funded":1,"publication":"Plant Physiology","abstract":[{"text":"Polar auxin transport plays a pivotal role in plant growth and development. PIN auxin efflux carriers regulate directional auxin movement by establishing local auxin maxima, minima, and gradients that drive multiple developmental processes and responses to environmental signals. Auxin has been proposed to modulate its own transport by regulating subcellular PIN trafficking via processes such as clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further investigated the mechanisms by which auxin affects PIN trafficking by screening auxin analogs and identified pinstatic acid (PISA) as a positive modulator of polar auxin transport in Arabidopsis thaliana. PISA had an auxin-like effect on hypocotyl elongation and adventitious root formation via positive regulation of auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN accumulation at the cell surface by inhibiting PIN internalization from the plasma membrane. This work demonstrates PISA to be a promising chemical tool to dissect the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.","lang":"eng"}],"external_id":{"isi":["000470086100045"],"pmid":["30936248"]},"article_processing_charge":"No","page":"1152-1165","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"06","citation":{"mla":"Oochi, A., et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.” <i>Plant Physiology</i>, vol. 180, no. 2, ASPB, 2019, pp. 1152–65, doi:<a href=\"https://doi.org/10.1104/pp.19.00201\">10.1104/pp.19.00201</a>.","ieee":"A. Oochi <i>et al.</i>, “Pinstatic acid promotes auxin transport by inhibiting PIN internalization,” <i>Plant Physiology</i>, vol. 180, no. 2. ASPB, pp. 1152–1165, 2019.","short":"A. Oochi, J. Hajny, K. Fukui, Y. Nakao, M.C. Gallei, M. Quareshy, K. Takahashi, T. Kinoshita, S. Harborough, S. Kepinski, H. Kasahara, R. Napier, J. Friml, K. Hayashi, Plant Physiology 180 (2019) 1152–1165.","chicago":"Oochi, A, Jakub Hajny, K Fukui, Y Nakao, Michelle C Gallei, M Quareshy, K Takahashi, et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.” <i>Plant Physiology</i>. ASPB, 2019. <a href=\"https://doi.org/10.1104/pp.19.00201\">https://doi.org/10.1104/pp.19.00201</a>.","ama":"Oochi A, Hajny J, Fukui K, et al. Pinstatic acid promotes auxin transport by inhibiting PIN internalization. <i>Plant Physiology</i>. 2019;180(2):1152-1165. doi:<a href=\"https://doi.org/10.1104/pp.19.00201\">10.1104/pp.19.00201</a>","ista":"Oochi A, Hajny J, Fukui K, Nakao Y, Gallei MC, Quareshy M, Takahashi K, Kinoshita T, Harborough S, Kepinski S, Kasahara H, Napier R, Friml J, Hayashi K. 2019. Pinstatic acid promotes auxin transport by inhibiting PIN internalization. Plant Physiology. 180(2), 1152–1165.","apa":"Oochi, A., Hajny, J., Fukui, K., Nakao, Y., Gallei, M. C., Quareshy, M., … Hayashi, K. (2019). Pinstatic acid promotes auxin transport by inhibiting PIN internalization. <i>Plant Physiology</i>. ASPB. <a href=\"https://doi.org/10.1104/pp.19.00201\">https://doi.org/10.1104/pp.19.00201</a>"},"scopus_import":"1","acknowledgement":"We thank Dr. H. Fukaki (University of Kobe), Dr. R. Offringa (Leiden University), Dr. Jianwei Pan (Zhejiang Normal University), and Dr. M. Estelle (University of California at San Diego) for providing mutants and transgenic line seeds.\r\nThis work was supported by the Ministry of Education, Culture, Sports, Science, and Technology (Grant-in-Aid for Scientific Research no. JP25114518 to K.H.), the Biotechnology and Biological Sciences Research Council (award no. BB/L009366/1 to R.N. and S.K.), and the European Union’s Horizon2020 program (European Research Council grant agreement no. 742985 to J.F.).","main_file_link":[{"url":"https://doi.org/10.1104/pp.19.00201","open_access":"1"}],"related_material":{"record":[{"id":"11626","status":"public","relation":"dissertation_contains"},{"id":"8822","relation":"dissertation_contains","status":"public"}]},"_id":"6260","publication_identifier":{"issn":["0032-0889"],"eissn":["1532-2548"]},"date_created":"2019-04-09T08:38:20Z","doi":"10.1104/pp.19.00201","oa_version":"Published Version","oa":1,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"2","author":[{"full_name":"Oochi, A","last_name":"Oochi","first_name":"A"},{"last_name":"Hajny","first_name":"Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","full_name":"Hajny, Jakub","orcid":"0000-0003-2140-7195"},{"first_name":"K","last_name":"Fukui","full_name":"Fukui, K"},{"first_name":"Y","last_name":"Nakao","full_name":"Nakao, Y"},{"orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","last_name":"Gallei"},{"last_name":"Quareshy","first_name":"M","full_name":"Quareshy, M"},{"first_name":"K","last_name":"Takahashi","full_name":"Takahashi, K"},{"first_name":"T","last_name":"Kinoshita","full_name":"Kinoshita, T"},{"last_name":"Harborough","first_name":"SR","full_name":"Harborough, SR"},{"full_name":"Kepinski, S","last_name":"Kepinski","first_name":"S"},{"full_name":"Kasahara, H","first_name":"H","last_name":"Kasahara"},{"first_name":"RM","last_name":"Napier","full_name":"Napier, RM"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml"},{"full_name":"Hayashi, KI","first_name":"KI","last_name":"Hayashi"}],"ddc":["580"],"date_published":"2019-06-01T00:00:00Z","intvolume":"       180","isi":1,"date_updated":"2026-06-27T22:31:02Z","publisher":"ASPB","article_type":"original","pmid":1,"publication_status":"published","status":"public","year":"2019","day":"01"},{"has_accepted_license":"1","intvolume":"        19","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"publist_id":"7950","author":[{"full_name":"Seitner, Denise","last_name":"Seitner","first_name":"Denise"},{"full_name":"Uhse, Simon","first_name":"Simon","last_name":"Uhse"},{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","last_name":"Gallei","first_name":"Michelle C"},{"full_name":"Djamei, Armin","last_name":"Djamei","first_name":"Armin"}],"ddc":["580"],"date_published":"2018-10-01T00:00:00Z","file":[{"access_level":"open_access","relation":"main_file","date_created":"2018-12-18T09:46:00Z","file_size":682335,"date_updated":"2018-12-18T09:46:00Z","content_type":"application/pdf","creator":"dernst","file_id":"5740","file_name":"2018_MolecPlantPath_Seitner.pdf","success":1}],"year":"2018","day":"01","date_updated":"2023-09-19T10:06:42Z","publisher":"Wiley","publication_status":"published","status":"public","page":"2277 - 2287","article_processing_charge":"No","external_id":{"isi":["000445624100006"]},"title":"The core effector Cce1 is required for early infection of maize by Ustilago maydis","volume":19,"publication":"Molecular Plant Pathology","abstract":[{"text":"The biotrophic pathogen Ustilago maydis, the causative agent of corn smut disease, infects one of the most important crops worldwide – Zea mays. To successfully colonize its host, U. maydis secretes proteins, known as effectors, that suppress plant defense responses and facilitate the establishment of biotrophy. In this work, we describe the U. maydis effector protein Cce1. Cce1 is essential for virulence and is upregulated during infection. Through microscopic analysis and in vitro assays, we show that Cce1 is secreted from hyphae during filamentous growth of the fungus. Strikingly, Δcce1 mutants are blocked at early stages of infection and induce callose deposition as a plant defense response. Cce1 is highly conserved among smut fungi and the Ustilago bromivora ortholog complemented the virulence defect of the SG200Δcce1 deletion strain. These data indicate that Cce1 is a core effector with apoplastic localization that is essential for U. maydis to infect its host.","lang":"eng"}],"file_date_updated":"2018-12-18T09:46:00Z","oa":1,"oa_version":"Published Version","department":[{"_id":"GradSch"}],"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"10","type":"journal_article","month":"10","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"apa":"Seitner, D., Uhse, S., Gallei, M. C., &#38; Djamei, A. (2018). The core effector Cce1 is required for early infection of maize by Ustilago maydis. <i>Molecular Plant Pathology</i>. Wiley. <a href=\"https://doi.org/10.1111/mpp.12698\">https://doi.org/10.1111/mpp.12698</a>","ista":"Seitner D, Uhse S, Gallei MC, Djamei A. 2018. The core effector Cce1 is required for early infection of maize by Ustilago maydis. Molecular Plant Pathology. 19(10), 2277–2287.","chicago":"Seitner, Denise, Simon Uhse, Michelle C Gallei, and Armin Djamei. “The Core Effector Cce1 Is Required for Early Infection of Maize by Ustilago Maydis.” <i>Molecular Plant Pathology</i>. Wiley, 2018. <a href=\"https://doi.org/10.1111/mpp.12698\">https://doi.org/10.1111/mpp.12698</a>.","ama":"Seitner D, Uhse S, Gallei MC, Djamei A. The core effector Cce1 is required for early infection of maize by Ustilago maydis. <i>Molecular Plant Pathology</i>. 2018;19(10):2277-2287. doi:<a href=\"https://doi.org/10.1111/mpp.12698\">10.1111/mpp.12698</a>","mla":"Seitner, Denise, et al. “The Core Effector Cce1 Is Required for Early Infection of Maize by Ustilago Maydis.” <i>Molecular Plant Pathology</i>, vol. 19, no. 10, Wiley, 2018, pp. 2277–87, doi:<a href=\"https://doi.org/10.1111/mpp.12698\">10.1111/mpp.12698</a>.","ieee":"D. Seitner, S. Uhse, M. C. Gallei, and A. Djamei, “The core effector Cce1 is required for early infection of maize by Ustilago maydis,” <i>Molecular Plant Pathology</i>, vol. 19, no. 10. Wiley, pp. 2277–2287, 2018.","short":"D. Seitner, S. Uhse, M.C. Gallei, A. Djamei, Molecular Plant Pathology 19 (2018) 2277–2287."},"scopus_import":"1","acknowledgement":"the Austrian Science Fund (FWF): [P27429‐B22, P27818‐B22, I 3033‐B22], and the Austrian Academy of Science (OEAW).","_id":"104","doi":"10.1111/mpp.12698","date_created":"2018-12-11T11:44:39Z"}]