[{"issue":"13","external_id":{"pmid":["42407441"]},"pmid":1,"quality_controlled":"1","date_created":"2026-07-13T09:39:40Z","doi":"10.1016/j.cub.2026.04.047","language":[{"iso":"eng"}],"intvolume":"        36","date_updated":"2026-07-13T11:11:56Z","scopus_import":"1","OA_type":"closed access","title":"Auxin and the control of plant growth and development","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"R739-R744","_id":"22286","volume":36,"citation":{"ieee":"V. Leitner and E. Benková, “Auxin and the control of plant growth and development,” <i>Current Biology</i>, vol. 36, no. 13. Elsevier, pp. R739–R744, 2026.","mla":"Leitner, Valentin, and Eva Benková. “Auxin and the Control of Plant Growth and Development.” <i>Current Biology</i>, vol. 36, no. 13, Elsevier, 2026, pp. R739–44, doi:<a href=\"https://doi.org/10.1016/j.cub.2026.04.047\">10.1016/j.cub.2026.04.047</a>.","short":"V. Leitner, E. Benková, Current Biology 36 (2026) R739–R744.","ista":"Leitner V, Benková E. 2026. Auxin and the control of plant growth and development. Current Biology. 36(13), R739–R744.","apa":"Leitner, V., &#38; Benková, E. (2026). Auxin and the control of plant growth and development. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2026.04.047\">https://doi.org/10.1016/j.cub.2026.04.047</a>","ama":"Leitner V, Benková E. Auxin and the control of plant growth and development. <i>Current Biology</i>. 2026;36(13):R739-R744. doi:<a href=\"https://doi.org/10.1016/j.cub.2026.04.047\">10.1016/j.cub.2026.04.047</a>","chicago":"Leitner, Valentin, and Eva Benková. “Auxin and the Control of Plant Growth and Development.” <i>Current Biology</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.cub.2026.04.047\">https://doi.org/10.1016/j.cub.2026.04.047</a>."},"publication":"Current Biology","publisher":"Elsevier","year":"2026","article_processing_charge":"No","abstract":[{"text":"Plants are remarkable organisms. Unlike animals, they cannot flee and, rooted in one place, they must cope with whatever challenges arise — nutrient scarcity, drought, shade, wind or obstacles in the soil. Their extraordinary ability to survive in such unstable environmental conditions lies in their capacity to adapt. In response to environmental signals, plants can rapidly adjust the rate of organ growth, change the direction of growth, bend toward resources, or remodel their body architecture by promoting or suppressing the formation of new organs such as lateral roots, branches, leaves, or flowers. This unique developmental plasticity depends on chemical signals, plant hormones that serve as regulators and coordinators of endogenous molecular and cellular processes. Chief among these signals is auxin, a plant hormone central to nearly every aspect of plant life.","lang":"eng"}],"month":"07","type":"journal_article","status":"public","date_published":"2026-07-06T00:00:00Z","publication_status":"published","oa_version":"None","supplementarymaterial":"no","publication_identifier":{"issn":["0960-9822"]},"das_tickbox":"0","day":"06","fulldoi":"https://doi.org/10.1016/j.cub.2026.04.047","corr_author":"1","department":[{"_id":"EvBe"},{"_id":"GradSch"}],"author":[{"first_name":"Valentin","id":"4c665ce3-0016-11ec-bea0-e44de7a4fa3d","last_name":"Leitner","full_name":"Leitner, Valentin"},{"orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva"}],"researchdata_availability":"no","article_type":"original"},{"publication_status":"published","dataavailabilitystatement":"The RNA-seq data generated in this study have been deposited in the GSA database under accession code CRA008967. The scRNA-seq data generated in this study have been deposited in the GSA database under accession code CRA026200. The ATAC-seq data generated in this study have been deposited in the GSA database under accession code CRA008969. The ChIP-seq data generated in this study have been deposited in the GSA database under accession code CRA008968. Single-cell RNA-seq data and scripts are publicly available on Zenodo (https://zenodo.org/records/20392012). Primers are in Supplemental Table 5. Source data are provided with this paper.","status":"public","date_published":"2026-08-07T00:00:00Z","file_date_updated":"2026-08-20T06:44:33Z","type":"journal_article","month":"08","abstract":[{"lang":"eng","text":"Ginseng (Panax ginseng) derives its renowned therapeutic properties from ginsenoside metabolites. However, the long cultivation cycle and susceptibility to diseases hinder the advancement of the ginseng industry. Here, we demonstrate that the embryonic protoderm of ginseng can efficiently produce ginsenosides. Single-cell transcriptome and mass spectrometry imaging analyses reveal that ginsenosides accumulate in the protoderm of ginseng embryonic callus (EC) at levels comparable to those in forest ginseng. Epigenetic analyses indicate that elevated histone acetylation and enhanced chromatin accessibility at regeneration- and ginsenoside metabolism-related gene loci are associated with the ginsenoside-producing capacity of EC. Increasing histone acetylation levels or overexpressing the regeneration-related WUSCHEL-RELATED HOMEOBOX11 (WOX11) gene further enhances ginsenoside production in EC. Our findings suggest that the protoderm of EC could serve as an in situ biological compartment for high-efficiency ginsenoside producion, offering a complementary approach to traditional ginseng cultivation."}],"article_processing_charge":"Yes","year":"2026","publisher":"Springer Nature","article_type":"original","researchdata_availability":"yes","author":[{"last_name":"Liu","full_name":"Liu, Juan","first_name":"Juan"},{"first_name":"Ning","full_name":"Zhai, Ning","last_name":"Zhai"},{"first_name":"Shiyi","full_name":"Zhang, Shiyi","last_name":"Zhang"},{"last_name":"Tamada","full_name":"Tamada, Yosuke","first_name":"Yosuke"},{"first_name":"Tonghui","last_name":"Li","full_name":"Li, Tonghui"},{"first_name":"Linfan","full_name":"Zhang, Linfan","last_name":"Zhang"},{"full_name":"Chen, Tong","last_name":"Chen","first_name":"Tong"},{"first_name":"Chenglin","full_name":"Wang, Chenglin","last_name":"Wang"},{"first_name":"Jian","full_name":"Yang, Jian","last_name":"Yang"},{"first_name":"Jiaqi","last_name":"Gao","full_name":"Gao, Jiaqi"},{"first_name":"Xiang","id":"4B7E523C-F248-11E8-B48F-1D18A9856A87","last_name":"Li","full_name":"Li, Xiang"},{"last_name":"Zhou","full_name":"Zhou, Junhui","first_name":"Junhui"},{"full_name":"Zhang, Yonghong","last_name":"Zhang","first_name":"Yonghong"},{"last_name":"Liu","full_name":"Liu, Yu","id":"2A70014E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7313-6740","first_name":"Yu"},{"first_name":"Yuan","full_name":"Wang, Yuan","last_name":"Wang"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"full_name":"Li, Chen","last_name":"Li","first_name":"Chen"},{"full_name":"Xu, Lin","last_name":"Xu","first_name":"Lin"},{"first_name":"Luqi","full_name":"Huang, Luqi","last_name":"Huang"}],"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"OA_place":"publisher","fulldoi":"https://doi.org/10.1038/s41467-026-74881-5","has_accepted_license":"1","day":"07","das_tickbox":"1","publication_identifier":{"eissn":["2041-1723"]},"supplementarymaterial":"yes","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"oa_version":"Published Version","ddc":["580"],"intvolume":"        17","language":[{"iso":"eng"}],"doi":"10.1038/s41467-026-74881-5","acknowledgement":"The authors are grateful to Professor Linfeng Li from the School of Life Science, Fudan University, for his assistance during the ginseng genome annotation. This work was supported by Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (2060302-2401-08 to L.X. and J.L.), the National Natural Science Foundation of China (82373987 to J.L., 31701294 to L.X., 32225007 to L.X., and 32300285 to N.Z.), the Fundamental Research Funds for the Central public welfare research institutes (ZZ13-YQ-093, ZZXT202508 to J.L.), the CACMS Innovation Fund (CI2025G00-06 to J.L.), the Principle Investigator Program (HBMUPI202104 to Y.Z.), the Key R&D Program of Shandong Province, China (2024LZGC025 to L.X.), the National Key R&D Program of China (2024YFF1000700/2023YFE0101100 to L.X.), Strategic Priority Research Program of Chinese Academy of Sciences (XDB0630000 to L.X.), and China Postdoctoral Science Foundation (2023M733490 to N.Z.).","date_created":"2026-08-16T22:01:42Z","quality_controlled":"1","pmid":1,"external_id":{"pmid":["42350386"]},"article_number":"7994","publication":"Nature Communications","citation":{"ama":"Liu J, Zhai N, Zhang S, et al. Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-74881-5\">10.1038/s41467-026-74881-5</a>","chicago":"Liu, Juan, Ning Zhai, Shiyi Zhang, Yosuke Tamada, Tonghui Li, Linfan Zhang, Tong Chen, et al. “Single-Cell Analyses Identify the Ginseng Embryonic Protoderm as a Native Compartment for High-Efficiency Ginsenoside Production.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-74881-5\">https://doi.org/10.1038/s41467-026-74881-5</a>.","apa":"Liu, J., Zhai, N., Zhang, S., Tamada, Y., Li, T., Zhang, L., … Huang, L. (2026). Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-74881-5\">https://doi.org/10.1038/s41467-026-74881-5</a>","short":"J. Liu, N. Zhai, S. Zhang, Y. Tamada, T. Li, L. Zhang, T. Chen, C. Wang, J. Yang, J. Gao, X. Li, J. Zhou, Y. Zhang, Y. Liu, Y. Wang, J. Friml, E. Benková, C. Li, L. Xu, L. Huang, Nature Communications 17 (2026).","ista":"Liu J, Zhai N, Zhang S, Tamada Y, Li T, Zhang L, Chen T, Wang C, Yang J, Gao J, Li X, Zhou J, Zhang Y, Liu Y, Wang Y, Friml J, Benková E, Li C, Xu L, Huang L. 2026. Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production. Nature Communications. 17, 7994.","mla":"Liu, Juan, et al. “Single-Cell Analyses Identify the Ginseng Embryonic Protoderm as a Native Compartment for High-Efficiency Ginsenoside Production.” <i>Nature Communications</i>, vol. 17, 7994, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-74881-5\">10.1038/s41467-026-74881-5</a>.","ieee":"J. Liu <i>et al.</i>, “Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026."},"file":[{"creator":"dernst","checksum":"0b4ff29f0d0168b11be32a5f22ded9e4","success":1,"date_updated":"2026-08-20T06:44:33Z","file_name":"2026_NatureComm_Liu.pdf","content_type":"application/pdf","relation":"main_file","file_id":"22742","file_size":2429551,"access_level":"open_access","date_created":"2026-08-20T06:44:33Z"}],"volume":17,"_id":"22712","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"title":"Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production","OA_type":"gold","DOAJ_listed":"1","scopus_import":"1","date_updated":"2026-08-20T06:45:46Z"},{"corr_author":"1","fulldoi":"https://doi.org/10.1016/j.devcel.2025.03.012","author":[{"full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"department":[{"_id":"EvBe"}],"article_type":"letter_note","oa_version":"None","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"day":"21","type":"journal_article","status":"public","date_published":"2025-04-21T00:00:00Z","publication_status":"published","year":"2025","article_processing_charge":"No","publisher":"Elsevier","abstract":[{"lang":"eng","text":"In this issue of Developmental Cell, Lee et al. identify a pivotal role for glutathione (GSH) in plant regeneration, a vital biological process enabling plants to regrow tissues and organs after injury. Applying single-cell RNA sequencing (scRNA-seq) and live imaging, the authors demonstrate that GSH, released upon tissue damage, accelerates cell-cycle transitions, particularly shortening the G1 phase, thereby facilitating efficient organ regeneration."}],"month":"04","page":"1137-1139","_id":"19594","volume":60,"citation":{"apa":"Benková, E. (2025). Unlocking plant regeneration: The role for glutathione. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.03.012\">https://doi.org/10.1016/j.devcel.2025.03.012</a>","ama":"Benková E. Unlocking plant regeneration: The role for glutathione. <i>Developmental Cell</i>. 2025;60(8):1137-1139. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.03.012\">10.1016/j.devcel.2025.03.012</a>","chicago":"Benková, Eva. “Unlocking Plant Regeneration: The Role for Glutathione.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.03.012\">https://doi.org/10.1016/j.devcel.2025.03.012</a>.","mla":"Benková, Eva. “Unlocking Plant Regeneration: The Role for Glutathione.” <i>Developmental Cell</i>, vol. 60, no. 8, Elsevier, 2025, pp. 1137–39, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.03.012\">10.1016/j.devcel.2025.03.012</a>.","ieee":"E. Benková, “Unlocking plant regeneration: The role for glutathione,” <i>Developmental Cell</i>, vol. 60, no. 8. Elsevier, pp. 1137–1139, 2025.","short":"E. Benková, Developmental Cell 60 (2025) 1137–1139.","ista":"Benková E. 2025. Unlocking plant regeneration: The role for glutathione. Developmental Cell. 60(8), 1137–1139."},"publication":"Developmental Cell","scopus_import":"1","date_updated":"2025-09-30T12:07:36Z","title":"Unlocking plant regeneration: The role for glutathione","OA_type":"closed access","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.1016/j.devcel.2025.03.012","language":[{"iso":"eng"}],"intvolume":"        60","isi":1,"issue":"8","pmid":1,"external_id":{"pmid":["40262524"],"isi":["001477400800001"]},"quality_controlled":"1","date_created":"2025-04-20T22:01:28Z"},{"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"publication_identifier":{"eissn":["1664-462X"]},"day":"04","has_accepted_license":"1","fulldoi":"https://doi.org/10.3389/fpls.2025.1612366","corr_author":"1","ec_funded":1,"OA_place":"publisher","author":[{"orcid":"0000-0003-4675-6893","first_name":"Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","last_name":"Gallemi","full_name":"Gallemi, Marçal"},{"full_name":"Montesinos López, Juan C","last_name":"Montesinos López","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9179-6099","first_name":"Juan C"},{"last_name":"Zarevski","full_name":"Zarevski, Nikola","id":"18e95355-e05a-11ea-a9c0-8fba1b89e83a","first_name":"Nikola"},{"last_name":"Pribyl","full_name":"Pribyl, Jan","first_name":"Jan"},{"first_name":"Petr","last_name":"Skládal","full_name":"Skládal, Petr"},{"full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva"}],"department":[{"_id":"EdHa"},{"_id":"EvBe"},{"_id":"CaGu"}],"article_type":"original","PlanS_conform":"1","publisher":"Frontiers Media","year":"2025","article_processing_charge":"Yes","abstract":[{"lang":"eng","text":"Introduction: Acid-growth theory has been postulated in the 70s to explain the rapid elongation of plant cells in response to the hormone auxin. More recently, it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting acidification of the apoplast is the principal mechanism by which auxin and other hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances, the impact of this acidification on the mechanical properties of the cell wall remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate hormone-induced tissue elongation and local changes in cell wall mechanical properties. Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase (PME), along with calcium chelators, we investigate the effect of pectin modification in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification of apoplast is necessary and sufficient to induce cell elongation through promoting cell wall softening. Moreover, we show that enhanced PME activity can induce both cell wall softening or stiffening in extracellular calcium dependent-manner and that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion: Our results confirm a dual role of PME in plant cell elongation. However, further investigation is needed to assess the status of pectin following short- or long-term PME treatments in order to determine if pectin methyl-esterification might promote its degradation as well as the role of PME inhibitors upon PME induction."}],"project":[{"grant_number":"207362","call_identifier":"FP7","name":"Hormonal cross-talk in plant organogenesis","_id":"253FCA6A-B435-11E9-9278-68D0E5697425"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"}],"month":"07","type":"journal_article","file_date_updated":"2025-07-31T07:28:54Z","status":"public","date_published":"2025-07-04T00:00:00Z","publication_status":"published","date_updated":"2026-05-20T07:53:03Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"}],"scopus_import":"1","DOAJ_listed":"1","APC_amount":"3642,79 EUR","title":"Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties","OA_type":"gold","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":16,"_id":"20080","citation":{"apa":"Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P., Hannezo, E. B., &#38; Benková, E. (2025). Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>","chicago":"Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl, Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2025. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>.","ama":"Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. 2025;16. doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>","mla":"Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>, vol. 16, 1612366, Frontiers Media, 2025, doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>.","ieee":"M. Gallemi <i>et al.</i>, “Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties,” <i>Frontiers in Plant Science</i>, vol. 16. Frontiers Media, 2025.","ista":"Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB, Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.","short":"M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B. Hannezo, E. Benková, Frontiers in Plant Science 16 (2025)."},"file":[{"file_size":3665187,"file_id":"20093","relation":"main_file","file_name":"2025_FrontiersPlantSc_Gallemi.pdf","content_type":"application/pdf","date_updated":"2025-07-31T07:28:54Z","date_created":"2025-07-31T07:28:54Z","access_level":"open_access","checksum":"9e6b8b53ba56d4a24a9bd91cf6d2dc58","creator":"dernst","success":1}],"publication":"Frontiers in Plant Science","article_number":"1612366","external_id":{"pmid":["40688689"],"isi":["001530690900001"]},"pmid":1,"quality_controlled":"1","acknowledgement":"The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127). We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler for the efforts to establish immunodetection method.","date_created":"2025-07-27T22:01:26Z","doi":"10.3389/fpls.2025.1612366","language":[{"iso":"eng"}],"isi":1,"intvolume":"        16","ddc":["580"]},{"OA_place":"publisher","ec_funded":1,"fulldoi":"https://doi.org/10.1093/plcell/koaf006","corr_author":"1","article_type":"original","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"author":[{"orcid":"0000-0003-1286-7368","first_name":"Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87","last_name":"Gallei","full_name":"Gallei, Michelle C"},{"last_name":"Truckenbrodt","full_name":"Truckenbrodt, Sven M","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","first_name":"Sven M"},{"id":"382077BA-F248-11E8-B48F-1D18A9856A87","first_name":"Caroline","last_name":"Kreuzinger","full_name":"Kreuzinger, Caroline"},{"first_name":"Syamala","orcid":"0009-0002-5890-120X","id":"F8660870-D756-11E9-98C5-34DFE5697425","full_name":"Inumella, Syamala","last_name":"Inumella"},{"full_name":"Vistunou, Vitali","last_name":"Vistunou","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","first_name":"Vitali"},{"id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","first_name":"Christoph M","full_name":"Sommer, Christoph M","last_name":"Sommer"},{"orcid":"0000-0002-7667-6854","first_name":"Mojtaba","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","last_name":"Tavakoli","full_name":"Tavakoli, Mojtaba"},{"first_name":"Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","last_name":"Agudelo Duenas","full_name":"Agudelo Duenas, Nathalie"},{"id":"937696FA-C996-11E9-8C7C-CF13E6697425","orcid":"0009-0000-7590-3501","first_name":"Jakob","last_name":"Vorlaufer","full_name":"Vorlaufer, Jakob"},{"id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","first_name":"Wiebke","orcid":"0000-0003-0201-2315","last_name":"Jahr","full_name":"Jahr, Wiebke"},{"last_name":"Randuch","full_name":"Randuch, Marek","first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"first_name":"Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson","full_name":"Johnson, Alexander J"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"first_name":"Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G","last_name":"Danzl"}],"related_material":{"record":[{"status":"public","id":"18837","relation":"research_data"},{"id":"18689","relation":"earlier_version","status":"public"}]},"publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","day":"01","file_date_updated":"2025-07-31T07:03:43Z","type":"journal_article","publication_status":"published","date_published":"2025-04-01T00:00:00Z","status":"public","abstract":[{"lang":"eng","text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. "}],"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","call_identifier":"H2020"},{"grant_number":"754411","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","_id":"269B5B22-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 679-2018"},{"grant_number":"26137","name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5"},{"grant_number":"W1232-B24","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular Drug Targets"}],"publisher":"Oxford University Press","PlanS_conform":"1","article_processing_charge":"Yes (via OA deal)","year":"2025","month":"04","_id":"19003","volume":37,"publication":"The Plant Cell","file":[{"date_updated":"2025-07-31T07:03:43Z","relation":"main_file","file_name":"2025_PlantCell_Gallei.pdf","content_type":"application/pdf","file_size":53904111,"file_id":"20092","access_level":"open_access","date_created":"2025-07-31T07:03:43Z","creator":"dernst","checksum":"9d3f8218ff37a29f29c48a7bbe831bd3","success":1}],"citation":{"short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006.","ieee":"M. C. Gallei <i>et al.</i>, “Super-resolution expansion microscopy in plant roots,” <i>The Plant Cell</i>, vol. 37, no. 4. Oxford University Press, 2025.","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>.","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. 2025;37(4). doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>","chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>.","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>"},"title":"Super-resolution expansion microscopy in plant roots","OA_type":"hybrid","oa":1,"date_updated":"2026-10-01T11:19:12Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}],"scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"doi":"10.1093/plcell/koaf006","ddc":["580"],"isi":1,"intvolume":"        37","external_id":{"isi":["001462763100001"],"pmid":["39792900"]},"pmid":1,"article_number":"koaf006","issue":"4","date_created":"2025-02-05T06:52:06Z","acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","quality_controlled":"1"},{"type":"journal_article","file_date_updated":"2025-09-01T14:09:31Z","status":"public","date_published":"2025-08-01T00:00:00Z","publication_status":"published","publisher":"Wiley","PlanS_conform":"1","year":"2025","article_processing_charge":"Yes (via OA deal)","abstract":[{"text":"Very long-chain fatty acids (VLCFAs), being constituents of different types of lipids, are critical factors in plant development, presumably due to their impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase 1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems (esm) mutant, a viable kcr1 allele presumably affecting surface properties of the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that impacts VLCFAs. The kcr1-2 plants show severe defects during different stages of development, which all correlate with defects in polar localization and subcellular trafficking of PIN auxin transporters and resulting asymmetric auxin distribution. Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests that KCR1 plays a role in delineating boundaries around meristematic and specialized differentiating tissues, including root and shoot meristems, initiating lateral roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful tool to study VLCFA roles in plant development and highlights VLCFAs as critical developmental factors at the interface of cell polarity and tissue development.","lang":"eng"}],"project":[{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","name":"Peptide receptors for auxin canalization in Arabidopsis","grant_number":"I06123"}],"month":"08","fulldoi":"https://doi.org/10.1111/tpj.70396","corr_author":"1","OA_place":"publisher","author":[{"first_name":"David","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","last_name":"Babic","full_name":"Babic, David"},{"orcid":"0000-0002-9357-9415","first_name":"Rashed","id":"4827E134-F248-11E8-B48F-1D18A9856A87","full_name":"Abualia, Rashed","last_name":"Abualia"},{"full_name":"Fiedler, Lukas","last_name":"Fiedler","id":"7c417475-8972-11ed-ae7b-8b674ca26986","first_name":"Lukas"},{"first_name":"Linlin","orcid":"0000-0001-5187-8401","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","last_name":"Qi","full_name":"Qi, Linlin"},{"last_name":"Tellier","full_name":"Tellier, Frédérique","first_name":"Frédérique"},{"first_name":"Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","last_name":"Smoljan","full_name":"Smoljan, Adrijana"},{"last_name":"Rakusova","full_name":"Rakusova, Hana","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA","first_name":"Hana"},{"last_name":"Valošek","full_name":"Valošek, Petr","first_name":"Petr","id":"3CDB6F94-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","full_name":"Han, Huibin","last_name":"Han"},{"last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Faure","full_name":"Faure, Jean Denis","first_name":"Jean Denis"},{"full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří"}],"department":[{"_id":"EvBe"},{"_id":"JiFr"},{"_id":"GradSch"}],"related_material":{"record":[{"relation":"dissertation_contains","id":"20362","status":"public"}]},"article_type":"original","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313X"]},"has_accepted_license":"1","day":"01","doi":"10.1111/tpj.70396","language":[{"iso":"eng"}],"isi":1,"intvolume":"       123","ddc":["580"],"article_number":"e70396","issue":"3","external_id":{"isi":["001547884300001"],"pmid":["40782342"]},"pmid":1,"quality_controlled":"1","acknowledgement":"We gratefully acknowledge the Imaging and Optics, Electron Microscopy (especially Vanessa Zheden for technical assistance) and Life Science (in particular Dorota Jaworska) facilities at ISTA for their continuous support. Authors would like to thank Michelle Gallei for advice during the generation of the transgenic lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski for advice on abiotic stress experiments. We would like to thank Dolf Weijers for the SOSEKI (SOK) marker line seeds. This work has benefited from the support of IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province (20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","date_created":"2025-08-17T22:01:36Z","volume":123,"_id":"20187","file":[{"checksum":"1cdc3341d2d23101abca72521f1f23cb","creator":"dernst","success":1,"file_name":"2025_PlantJournal_Babic.pdf","relation":"main_file","content_type":"application/pdf","file_size":5791111,"file_id":"20264","date_updated":"2025-09-01T14:09:31Z","access_level":"open_access","date_created":"2025-09-01T14:09:31Z"}],"citation":{"ista":"Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 123(3), e70396.","short":"D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova, P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).","ieee":"D. Babic <i>et al.</i>, “Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development,” <i>Plant Journal</i>, vol. 123, no. 3. Wiley, 2025.","mla":"Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant Journal</i>, vol. 123, no. 3, e70396, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/tpj.70396\">10.1111/tpj.70396</a>.","chicago":"Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier, Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant Journal</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/tpj.70396\">https://doi.org/10.1111/tpj.70396</a>.","ama":"Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>. 2025;123(3). doi:<a href=\"https://doi.org/10.1111/tpj.70396\">10.1111/tpj.70396</a>","apa":"Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml, J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.70396\">https://doi.org/10.1111/tpj.70396</a>"},"publication":"Plant Journal","date_updated":"2026-10-16T07:59:32Z","scopus_import":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"OA_type":"hybrid","oa":1,"title":"Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"type":"other_academic_publication","status":"public","date_published":"2024-01-08T00:00:00Z","publication_status":"published","publisher":"Elsevier","article_processing_charge":"No","year":"2024","abstract":[{"text":"Eva Benkova received a PhD in Biophysics at the Institute of Biophysics of the Czech Academy of Sciences in 1998. After working as a postdoc at the Max Planck Institute in Cologne and the Center for Plant Molecular Biology (ZMBP) in Tübingen, she became a group leader at the Plant Systems Biology Department of the Vlaams Instituut voor Biotechnologie (VIB) in Gent. In 2012, she transitioned to an Assistant Professor position at the Institute of Science and Technology Austria (ISTA) where she was later promoted to Professor. Since 2021, she has served as the Dean of the ISTA Graduate School. As a plant developmental biologist, she focuses on unraveling the molecular mechanisms and principles that underlie hormonal interactions in plants. In her current work, she explores the intricate connections between hormones and regulatory pathways that mediate the perception of environmental stimuli, including abiotic stress and nitrate availability.","lang":"eng"}],"month":"01","fulldoi":"https://doi.org/10.1016/j.cub.2023.11.039","corr_author":"1","department":[{"_id":"EvBe"}],"author":[{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva"}],"researchdata_availability":"not applicable","oa_version":"Published Version","supplementarymaterial":"no","publication_identifier":{"eissn":["1879-0445"]},"das_tickbox":"0","day":"08","doi":"10.1016/j.cub.2023.11.039","language":[{"iso":"eng"}],"intvolume":"        34","ddc":["580"],"issue":"1","external_id":{"pmid":["38194926"]},"pmid":1,"quality_controlled":"1","date_created":"2024-01-21T23:00:56Z","page":"R3-R5","_id":"14842","volume":34,"citation":{"apa":"Benková, E. (2024). <i>Eva Benkova</i>. <i>Current Biology</i> (Vol. 34, pp. R3–R5). Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2023.11.039\">https://doi.org/10.1016/j.cub.2023.11.039</a>","chicago":"Benková, Eva. <i>Eva Benkova</i>. <i>Current Biology</i>. Vol. 34. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2023.11.039\">https://doi.org/10.1016/j.cub.2023.11.039</a>.","ama":"Benková E. <i>Eva Benkova</i>. Vol 34. Elsevier; 2024:R3-R5. doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.039\">10.1016/j.cub.2023.11.039</a>","mla":"Benková, Eva. “Eva Benkova.” <i>Current Biology</i>, vol. 34, no. 1, Elsevier, 2024, pp. R3–5, doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.039\">10.1016/j.cub.2023.11.039</a>.","ieee":"E. Benková, <i>Eva Benkova</i>, vol. 34, no. 1. Elsevier, 2024, pp. R3–R5.","ista":"Benková E. 2024. Eva Benkova, Elsevier,p.","short":"E. Benková, Eva Benkova, Elsevier, 2024."},"publication":"Current Biology","date_updated":"2026-10-01T11:17:58Z","oa":1,"title":"Eva Benkova","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.cub.2023.11.039"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345"},{"_id":"18689","publication":"bioRxiv","citation":{"chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2024.02.21.581330\">https://doi.org/10.1101/2024.02.21.581330</a>.","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (n.d.). Super-resolution expansion microscopy in plant roots. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2024.02.21.581330\">https://doi.org/10.1101/2024.02.21.581330</a>","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. Super-resolution expansion microscopy in plant roots. bioRxiv, <a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>.","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, BioRxiv (n.d.).","ieee":"M. C. Gallei <i>et al.</i>, “Super-resolution expansion microscopy in plant roots,” <i>bioRxiv</i>. .","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2024.02.21.581330\">10.1101/2024.02.21.581330</a>."},"title":"Super-resolution expansion microscopy in plant roots","oa":1,"date_updated":"2026-10-02T11:27:43Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","main_file_link":[{"url":"https://doi.org/10.1101/2024.02.21.581330","open_access":"1"}],"language":[{"iso":"eng"}],"doi":"10.1101/2024.02.21.581330","date_created":"2024-12-19T12:28:00Z","acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\n\r\nThis project has received funding from the Austrian Science Fund (FWF): I 3630-B25 (J.G.D) and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 742985, J.F.). It has also received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385. S.T. has received funding as an ISTplus Fellow from the European Union’s Horizon 2020 Research and Innovation Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from an EMBO Long-Term Fellowship (grant number ALTF 679-2018). It has further received funding from the Austrian Science Fund (FWF) grant DK W1232 (M.T, N.A-D., J.G.D). W.J. received funding via a Human Frontier Science Program postdoctoral fellowship LT000557/2018.\r\n\r\nThe funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","OA_place":"repository","ec_funded":1,"fulldoi":"https://doi.org/10.1101/2024.02.21.581330","corr_author":"1","author":[{"last_name":"Gallei","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","first_name":"Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sven M","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","last_name":"Truckenbrodt","full_name":"Truckenbrodt, Sven M"},{"first_name":"Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87","full_name":"Kreuzinger, Caroline","last_name":"Kreuzinger"},{"first_name":"Syamala","orcid":"0009-0002-5890-120X","id":"F8660870-D756-11E9-98C5-34DFE5697425","last_name":"Inumella","full_name":"Inumella, Syamala"},{"first_name":"Vitali","id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","full_name":"Vistunou, Vitali","last_name":"Vistunou"},{"orcid":"0000-0003-1216-9105","first_name":"Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M","last_name":"Sommer"},{"id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","first_name":"Mojtaba","orcid":"0000-0002-7667-6854","full_name":"Tavakoli, Mojtaba","last_name":"Tavakoli"},{"first_name":"Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","last_name":"Agudelo Duenas","full_name":"Agudelo Duenas, Nathalie"},{"id":"937696FA-C996-11E9-8C7C-CF13E6697425","first_name":"Jakob","orcid":"0009-0000-7590-3501","full_name":"Vorlaufer, Jakob","last_name":"Vorlaufer"},{"full_name":"Jahr, Wiebke","last_name":"Jahr","orcid":"0000-0003-0201-2315","first_name":"Wiebke","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Randuch, Marek","last_name":"Randuch","first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae"},{"orcid":"0000-0002-2739-8843","first_name":"Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","full_name":"Johnson, Alexander J","last_name":"Johnson"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková"},{"full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-8559-3973","first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G","last_name":"Danzl"}],"department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"researchdata_availability":"no","related_material":{"record":[{"status":"public","relation":"later_version","id":"19003"},{"id":"18681","relation":"dissertation_contains","status":"public"}]},"oa_version":"Preprint","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)"},"supplementarymaterial":"no","day":"21","das_tickbox":"0","type":"preprint","publication_status":"draft","date_published":"2024-02-21T00:00:00Z","status":"public","abstract":[{"text":"Multiplexed fluorescence microscopy imaging is widely used in biomedical applications. However, simultaneous imaging of multiple fluorophores can result in spectral leaks and overlapping, which greatly degrades image quality and subsequent analysis. Existing popular spectral unmixing methods are mainly based on computational intensive linear models and the performance is heavily dependent on the reference spectra, which may greatly preclude its further applications. In this paper, we propose a deep learning-based blindly spectral unmixing method, termed AutoUnmix, to imitate the physical spectral mixing process. A tranfer learning framework is further devised to allow our AutoUnmix adapting to a variety of imaging systems without retraining the network. Our proposed method has demonstrated real-time unmixing capabilities, surpassing existing methods by up to 100-fold in terms of unmixing speed. We further validate the reconstruction performance on both synthetic datasets and biological samples. The unmixing results of AutoUnmix achieve a highest SSIM of 0.99 in both three- and four-color imaging, with nearly up to 20% higher than other popular unmixing methods. Due to the desirable property of data independency and superior blind unmixing performance, we believe AutoUnmix is a powerful tool to study the interaction process of different organelles labeled by multiple fluorophores.","lang":"eng"}],"project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"grant_number":"665385","call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020","grant_number":"754411"},{"grant_number":"W1232-B24","call_identifier":"FWF","name":"Molecular Drug Targets","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425"},{"grant_number":"ALTF 679-2018","name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED","_id":"269B5B22-B435-11E9-9278-68D0E5697425"}],"year":"2024","article_processing_charge":"No","month":"02"},{"ec_funded":1,"corr_author":"1","fulldoi":"https://doi.org/10.1016/j.devcel.2024.03.009","article_type":"original","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/how-plants-heal-wounds/"}]},"researchdata_availability":"upon request","department":[{"_id":"JiFr"},{"_id":"EvBe"},{"_id":"CaHe"}],"author":[{"last_name":"Hörmayer","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926","first_name":"Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87"},{"id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9179-6099","first_name":"Juan C","last_name":"Montesinos López","full_name":"Montesinos López, Juan C"},{"full_name":"Trozzi, N","last_name":"Trozzi","first_name":"N"},{"full_name":"Spona, Leonhard","last_name":"Spona","first_name":"Leonhard","id":"b52391fb-f636-11ee-939c-8a8c47552e8a"},{"orcid":"0000-0001-6111-9353","first_name":"Saiko","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","last_name":"Yoshida","full_name":"Yoshida, Saiko"},{"full_name":"Marhavá, Petra","last_name":"Marhavá","first_name":"Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Caballero Mancebo, Silvia","last_name":"Caballero Mancebo","first_name":"Silvia","orcid":"0000-0002-5223-3346","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"},{"first_name":"Y","last_name":"Dagdas","full_name":"Dagdas, Y"},{"first_name":"M","full_name":"Majda, M","last_name":"Majda"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml"}],"publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"supplementarymaterial":"yes","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","day":"20","das_tickbox":"1","file_date_updated":"2024-08-20T11:22:16Z","type":"journal_article","publication_status":"published","dataavailabilitystatement":"All data reported in this paper will be shared by the lead contact upon request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","date_published":"2024-05-20T00:00:00Z","status":"public","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"},{"_id":"262EF96E-B435-11E9-9278-68D0E5697425","name":"RNA-directed DNA methylation in plant development","call_identifier":"FWF","grant_number":"P29988"}],"abstract":[{"text":"Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture.","lang":"eng"}],"year":"2024","article_processing_charge":"Yes (via OA deal)","publisher":"Elsevier","month":"05","_id":"15301","volume":59,"page":"1333-1344.e4","publication":"Developmental Cell","file":[{"date_updated":"2024-08-20T11:22:16Z","file_id":"17452","file_size":5195262,"file_name":"2024_DevelopmentalCell_Hoermayer.pdf","relation":"main_file","content_type":"application/pdf","date_created":"2024-08-20T11:22:16Z","access_level":"open_access","creator":"dernst","checksum":"22b374fb50a40d380b7686c84258d271","success":1}],"citation":{"ama":"Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. 2024;59(10):1333-1344.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>","chicago":"Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>.","apa":"Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S., Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>","short":"L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá, S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J. Friml, Developmental Cell 59 (2024) 1333–1344.e4.","ista":"Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P, Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 59(10), 1333–1344.e4.","mla":"Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>, vol. 59, no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>.","ieee":"L. Hörmayer <i>et al.</i>, “Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization,” <i>Developmental Cell</i>, vol. 59, no. 10. Elsevier, p. 1333–1344.e4, 2024."},"title":"Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization","oa":1,"scopus_import":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"date_updated":"2026-10-15T08:32:32Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","language":[{"iso":"eng"}],"doi":"10.1016/j.devcel.2024.03.009","ddc":["570"],"intvolume":"        59","isi":1,"pmid":1,"external_id":{"isi":["001301584600001"],"pmid":["38579717"]},"issue":"10","date_created":"2024-04-08T12:07:57Z","acknowledgement":"We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder for sharing published material. We thank the Imaging & Optics and Life Science Facilities at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility at DBMV UNIL for providing invaluable assistance. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985, from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).","quality_controlled":"1"},{"month":"12","abstract":[{"lang":"eng","text":"Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsis thaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int7 results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis."}],"article_processing_charge":"Yes (in subscription journal)","year":"2024","publisher":"Elsevier","publication_status":"published","date_published":"2024-12-02T00:00:00Z","status":"public","file_date_updated":"2024-12-03T11:08:09Z","type":"journal_article","has_accepted_license":"1","day":"02","das_tickbox":"0","publication_identifier":{"issn":["1674-2052"]},"supplementarymaterial":"yes","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"article_type":"original","researchdata_availability":"no","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"author":[{"full_name":"Králová, Michaela","last_name":"Králová","first_name":"Michaela"},{"first_name":"Ivona","full_name":"Kubalová, Ivona","last_name":"Kubalová"},{"first_name":"Jakub","full_name":"Hajný, Jakub","last_name":"Hajný"},{"id":"946011F4-3E71-11EA-860B-C7A73DDC885E","first_name":"Karolina","orcid":"0000-0001-5630-9419","full_name":"Kubiasova, Karolina","last_name":"Kubiasova"},{"last_name":"Vagaská","full_name":"Vagaská, Karolína","first_name":"Karolína"},{"full_name":"Ge, Zengxiang","last_name":"Ge","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","orcid":"0000-0001-9381-3577","first_name":"Zengxiang"},{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","first_name":"Michelle C","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","last_name":"Gallei"},{"id":"42FE702E-F248-11E8-B48F-1D18A9856A87","first_name":"Hana","last_name":"Semerádová","full_name":"Semerádová, Hana"},{"first_name":"Anna","last_name":"Kuchařová","full_name":"Kuchařová, Anna"},{"full_name":"Hönig, Martin","last_name":"Hönig","first_name":"Martin"},{"id":"2DB5D88C-D7B3-11E9-B8FD-7907E6697425","first_name":"Aline","last_name":"Monzer","full_name":"Monzer, Aline"},{"first_name":"Martin","last_name":"Kovačik","full_name":"Kovačik, Martin"},{"full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ondřej","last_name":"Novák","full_name":"Novák, Ondřej"},{"last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva"},{"last_name":"Ikeda","full_name":"Ikeda, Yoshihisa","first_name":"Yoshihisa"},{"last_name":"Zalabák","full_name":"Zalabák, David","first_name":"David"}],"OA_place":"publisher","fulldoi":"https://doi.org/10.1016/j.molp.2024.11.001","date_created":"2024-11-28T11:13:35Z","acknowledgement":"We dedicate this paper to the deceased Petr Galuszka for his inspiration and support of our project. We thank Prof. Peter Hedden for constructive criticism of the manuscript and English editing. No conflict of interest is declared.","quality_controlled":"1","pmid":1,"external_id":{"pmid":["39501563"],"isi":["001373778300001"]},"issue":"12","ddc":["580"],"intvolume":"        17","isi":1,"language":[{"iso":"eng"}],"doi":"10.1016/j.molp.2024.11.001","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","oa":1,"OA_type":"hybrid","title":"A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"scopus_import":"1","date_updated":"2026-10-15T08:30:20Z","publication":"Molecular Plant","citation":{"apa":"Králová, M., Kubalová, I., Hajný, J., Kubiasova, K., Vagaská, K., Ge, Z., … Zalabák, D. (2024). A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>","ama":"Králová M, Kubalová I, Hajný J, et al. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. <i>Molecular Plant</i>. 2024;17(12):1850-1865. doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>","chicago":"Králová, Michaela, Ivona Kubalová, Jakub Hajný, Karolina Kubiasova, Karolína Vagaská, Zengxiang Ge, Michelle C Gallei, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">https://doi.org/10.1016/j.molp.2024.11.001</a>.","ieee":"M. Králová <i>et al.</i>, “A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis,” <i>Molecular Plant</i>, vol. 17, no. 12. Elsevier, pp. 1850–1865, 2024.","mla":"Králová, Michaela, et al. “A Decoy Receptor Derived from Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” <i>Molecular Plant</i>, vol. 17, no. 12, Elsevier, 2024, pp. 1850–65, doi:<a href=\"https://doi.org/10.1016/j.molp.2024.11.001\">10.1016/j.molp.2024.11.001</a>.","short":"M. Králová, I. Kubalová, J. Hajný, K. Kubiasova, K. Vagaská, Z. Ge, M.C. Gallei, H. Semerádová, A. Kuchařová, M. Hönig, A. Monzer, M. Kovačik, J. Friml, O. Novák, E. Benková, Y. Ikeda, D. Zalabák, Molecular Plant 17 (2024) 1850–1865.","ista":"Králová M, Kubalová I, Hajný J, Kubiasova K, Vagaská K, Ge Z, Gallei MC, Semerádová H, Kuchařová A, Hönig M, Monzer A, Kovačik M, Friml J, Novák O, Benková E, Ikeda Y, Zalabák D. 2024. A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. Molecular Plant. 17(12), 1850–1865."},"file":[{"access_level":"open_access","date_created":"2024-12-03T11:08:09Z","date_updated":"2024-12-03T11:08:09Z","relation":"main_file","file_name":"2024_MolecularPlant_Kralova.pdf","content_type":"application/pdf","file_id":"18612","file_size":3308945,"success":1,"creator":"dernst","checksum":"a11feea4b1677df76b632eca04bfc1dd"}],"volume":17,"_id":"18596","page":"1850-1865"},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","scopus_import":"1","date_updated":"2026-10-15T22:31:28Z","oa":1,"title":"Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis","citation":{"apa":"Peng, Y., Ji, K., Mao, Y., Wang, Y., Korbei, B., Luschnig, C., … Tan, S. (2024). Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-024-06747-9\">https://doi.org/10.1038/s42003-024-06747-9</a>","ama":"Peng Y, Ji K, Mao Y, et al. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. <i>Communications Biology</i>. 2024;7. doi:<a href=\"https://doi.org/10.1038/s42003-024-06747-9\">10.1038/s42003-024-06747-9</a>","chicago":"Peng, Yakun, Kangkang Ji, Yanbo Mao, Yiqun Wang, Barbara Korbei, Christian Luschnig, Jinbo Shen, Eva Benková, Jiří Friml, and Shutang Tan. “Polarly Localized Bro1 Domain Proteins Regulate PIN-FORMED Abundance and Root Gravitropic Growth in Arabidopsis.” <i>Communications Biology</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s42003-024-06747-9\">https://doi.org/10.1038/s42003-024-06747-9</a>.","ieee":"Y. Peng <i>et al.</i>, “Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis,” <i>Communications Biology</i>, vol. 7. Springer Nature, 2024.","mla":"Peng, Yakun, et al. “Polarly Localized Bro1 Domain Proteins Regulate PIN-FORMED Abundance and Root Gravitropic Growth in Arabidopsis.” <i>Communications Biology</i>, vol. 7, 1085, Springer Nature, 2024, doi:<a href=\"https://doi.org/10.1038/s42003-024-06747-9\">10.1038/s42003-024-06747-9</a>.","short":"Y. Peng, K. Ji, Y. Mao, Y. Wang, B. Korbei, C. Luschnig, J. Shen, E. Benková, J. Friml, S. Tan, Communications Biology 7 (2024).","ista":"Peng Y, Ji K, Mao Y, Wang Y, Korbei B, Luschnig C, Shen J, Benková E, Friml J, Tan S. 2024. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis. Communications Biology. 7, 1085."},"file":[{"creator":"dernst","checksum":"7d66af41c90e73d1b8a375eb652a9561","success":1,"date_updated":"2024-09-17T09:44:29Z","file_size":7718758,"file_id":"18084","content_type":"application/pdf","file_name":"2024_CommBiology_Peng.pdf","relation":"main_file","date_created":"2024-09-17T09:44:29Z","access_level":"open_access"}],"publication":"Communications Biology","volume":7,"_id":"18063","quality_controlled":"1","date_created":"2024-09-15T22:01:38Z","acknowledgement":"We thank Drs. Erika Isono (University of Constance), Grégory Vert (University of Toulouse), and Liwen Jiang (The Chinese University of Hong Kong) for kindly sharing published Arabidopsis lines; Dr. Yuzhou Zhang (ISTA) for help with molecular cloning, and Drs. Melinda Abas (BOKU), Eugenia Russinova (Ghent University), and Zhaojun Ding (Shandong University) for valuable discussions. This work was supported by grants to S.T. from the National Natural Science Foundation of China (32321001), the USTC Research Funds of the Double First-Class Initiative (YD9100002016), the Research Funds from the Center for Advanced Interdisciplinary Science and Biomedicine of IHM, the Division of Life Sciences and Medicine, the University of Science and Technology of China (QYPY20220012), the Fundamental Research Funds for the Central Universities (WK9100000021), and start-up funding from the University of Science and Technology of China and the Chinese Academy of Sciences (GG9100007007, KY9100000026, KY9100000051, and KJ2070000079). J.S. was supported by the National Natural Science Foundation of China (31970181 and 32170342). J.F. was supported by Austrian Science Fund (FWF; projects I6123 and P37051-B).","article_number":"1085","pmid":1,"external_id":{"isi":["001306499600002"],"pmid":["39232040"]},"intvolume":"         7","isi":1,"ddc":["570"],"doi":"10.1038/s42003-024-06747-9","language":[{"iso":"eng"}],"das_tickbox":"1","day":"04","has_accepted_license":"1","supplementarymaterial":"yes","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"publication_identifier":{"eissn":["2399-3642"]},"related_material":{"record":[{"id":"20117","relation":"dissertation_contains","status":"public"}]},"researchdata_availability":"yes (supplementary data)","author":[{"first_name":"Yakun","last_name":"Peng","full_name":"Peng, Yakun"},{"first_name":"Kangkang","last_name":"Ji","full_name":"Ji, Kangkang"},{"first_name":"Yanbo","last_name":"Mao","full_name":"Mao, Yanbo"},{"last_name":"Wang","full_name":"Wang, Yiqun","id":"82F537F2-B517-11E9-84D7-6433E6697425","first_name":"Yiqun"},{"first_name":"Barbara","last_name":"Korbei","full_name":"Korbei, Barbara"},{"full_name":"Luschnig, Christian","last_name":"Luschnig","first_name":"Christian"},{"full_name":"Shen, Jinbo","last_name":"Shen","first_name":"Jinbo"},{"last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Tan","full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","first_name":"Shutang","orcid":"0000-0002-0471-8285"}],"department":[{"_id":"EvBe"},{"_id":"JiFr"}],"article_type":"original","fulldoi":"https://doi.org/10.1038/s42003-024-06747-9","month":"09","year":"2024","article_processing_charge":"Yes","publisher":"Springer Nature","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123"},{"grant_number":"P37051","name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6"}],"abstract":[{"text":"The developmental plasticity of the root system plays an essential role in the adaptation of plants to the environment. Among many other signals, auxin and its directional, intercellular transport are critical in regulating root growth and development. In particular, the PIN-FORMED2 (PIN2) auxin exporter acts as a key regulator of root gravitropic growth. Multiple regulators have been reported to be involved in PIN2-mediated root growth; however, our information remains incomplete. Here, we identified ROWY Bro1-domain proteins as important regulators of PIN2 sorting control. Genetic analysis revealed that Arabidopsis rowy1 single mutants and higher-order rowy1 rowy2 rowy3 triple mutants presented a wavy root growth phenotype. Cell biological experiments revealed that ROWY1 and PIN2 colocalized to the apical side of the plasma membrane in the root epidermis and that ROWYs are required for correct PM targeting of PIN2. In addition, ROWYs also affected PIN3 protein abundance in the stele, suggesting the potential involvement of additional PIN transporters as well as other proteins. A global transcriptome analysis revealed that ROWY genes are involved in the Fe2+ availability perception pathway. This work establishes ROWYs as important novel regulators of root gravitropic growth by connecting micronutrient availability to the proper subcellular targeting of PIN auxin transporters.","lang":"eng"}],"dataavailabilitystatement":"The RNA-seq data for this study are available online at the NCBI Sequence Read Archive (accession number: PRJNA1116831). Uncropped images of the DNA gels are provided in the Supplementary Figs. 21 to 22. The source data used to generate the plots are provided in the Supplementary Data 2.","status":"public","date_published":"2024-09-04T00:00:00Z","publication_status":"published","type":"journal_article","file_date_updated":"2024-09-17T09:44:29Z"},{"ddc":["570"],"intvolume":"        12","isi":1,"language":[{"iso":"eng"}],"doi":"10.3390/cells12121613","date_created":"2023-07-12T07:41:25Z","acknowledgement":"This work was supported by the Austrian Academy of Sciences ÖAW: Doc fellowship (26130) to Stefan Riegler.","quality_controlled":"1","pmid":1,"external_id":{"pmid":["37371083"],"isi":["001017033600001"]},"issue":"12","article_number":"1613","publication":"Cells","citation":{"ista":"Abualia R, Riegler S, Benková E. 2023. Nitrate, auxin and cytokinin - a trio to tango. Cells. 12(12), 1613.","short":"R. Abualia, S. Riegler, E. Benková, Cells 12 (2023).","ieee":"R. Abualia, S. Riegler, and E. Benková, “Nitrate, auxin and cytokinin - a trio to tango,” <i>Cells</i>, vol. 12, no. 12. MDPI, 2023.","mla":"Abualia, R., et al. “Nitrate, Auxin and Cytokinin - a Trio to Tango.” <i>Cells</i>, vol. 12, no. 12, 1613, MDPI, 2023, doi:<a href=\"https://doi.org/10.3390/cells12121613\">10.3390/cells12121613</a>.","chicago":"Abualia, R, Stefan Riegler, and Eva Benková. “Nitrate, Auxin and Cytokinin - a Trio to Tango.” <i>Cells</i>. MDPI, 2023. <a href=\"https://doi.org/10.3390/cells12121613\">https://doi.org/10.3390/cells12121613</a>.","ama":"Abualia R, Riegler S, Benková E. Nitrate, auxin and cytokinin - a trio to tango. <i>Cells</i>. 2023;12(12). doi:<a href=\"https://doi.org/10.3390/cells12121613\">10.3390/cells12121613</a>","apa":"Abualia, R., Riegler, S., &#38; Benková, E. (2023). Nitrate, auxin and cytokinin - a trio to tango. <i>Cells</i>. MDPI. <a href=\"https://doi.org/10.3390/cells12121613\">https://doi.org/10.3390/cells12121613</a>"},"file":[{"checksum":"6dc9df5f4f59fc27c509c275060354a5","creator":"alisjak","success":1,"file_id":"13218","file_size":1066802,"relation":"main_file","content_type":"application/pdf","file_name":"2023_cells_Abualia.pdf","date_updated":"2023-07-12T10:01:54Z","date_created":"2023-07-12T10:01:54Z","access_level":"open_access"}],"volume":12,"_id":"13214","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","title":"Nitrate, auxin and cytokinin - a trio to tango","oa":1,"scopus_import":"1","date_updated":"2025-04-15T06:27:18Z","publication_status":"published","date_published":"2023-06-13T00:00:00Z","status":"public","file_date_updated":"2023-07-12T10:01:54Z","type":"journal_article","month":"06","project":[{"grant_number":"26130","_id":"62883ed7-2b32-11ec-9570-93580204e56b","name":"Functional asymmetry of medial habenula outputs in mice"}],"abstract":[{"text":"Nitrogen is an important macronutrient required for plant growth and development, thus directly impacting agricultural productivity. In recent years, numerous studies have shown that nitrogen-driven growth depends on pathways that control nitrate/nitrogen homeostasis and hormonal networks that act both locally and systemically to coordinate growth and development of plant organs. In this review, we will focus on recent advances in understanding the role of the plant hormones auxin and cytokinin and their crosstalk in nitrate-regulated growth and discuss the significance of novel findings and possible missing links.","lang":"eng"}],"article_processing_charge":"Yes","year":"2023","publisher":"MDPI","article_type":"review","author":[{"first_name":"R","last_name":"Abualia","full_name":"Abualia, R"},{"last_name":"Riegler","full_name":"Riegler, Stefan","id":"FF6018E0-D806-11E9-8E43-0B14E6697425","first_name":"Stefan","orcid":"0000-0003-3413-1343"},{"full_name":"Benková, Eva","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva"}],"department":[{"_id":"EvBe"}],"corr_author":"1","fulldoi":"https://doi.org/10.3390/cells12121613","day":"13","has_accepted_license":"1","publication_identifier":{"issn":["2073-4409"]},"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"}},{"external_id":{"pmid":["35878040"],"isi":["000881496900007"]},"pmid":1,"issue":"31","article_number":"e2122460119","date_created":"2022-08-07T22:01:57Z","acknowledgement":"We acknowledge Hana Semeradova, Juan Carlos Montesinos, Nicola Cavallari, Marc¸al Gallem\u0003ı, Kaori Tabata, Andrej Hurn\u0003y, and Sascha Waidmann for sharing materials; and Marina Borges Osorio for critical reading of the manuscript. Work in the E. Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S) to K.O., R.A., and E. Benkova. We acknowledge the Bioimaging Facility and Life Science Facilities of the Institute of Science\r\nand Technology Austria. We give sincere thanks to Hana Martınkova and Petra Amakorova for their help with cytokinin analyses. This work was funded by the Czech Science Foundation (Project No. 19-00973S).","quality_controlled":"1","language":[{"iso":"eng"}],"doi":"10.1073/pnas.2122460119","ddc":["570"],"isi":1,"intvolume":"       119","title":"Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses","oa":1,"date_updated":"2025-05-14T11:00:29Z","scopus_import":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":119,"_id":"11734","publication":"Proceedings of the National Academy of Sciences of the United States of America","citation":{"short":"R. Abualia, K. Ötvös, O. Novák, E. Bouguyon, K. Domanegg, A. Krapp, P. Nacry, A. Gojon, B. Lacombe, E. Benková, Proceedings of the National Academy of Sciences of the United States of America 119 (2022).","ista":"Abualia R, Ötvös K, Novák O, Bouguyon E, Domanegg K, Krapp A, Nacry P, Gojon A, Lacombe B, Benková E. 2022. Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. Proceedings of the National Academy of Sciences of the United States of America. 119(31), e2122460119.","mla":"Abualia, Rashed, et al. “Molecular Framework Integrating Nitrate Sensing in Root and Auxin-Guided Shoot Adaptive Responses.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 119, no. 31, e2122460119, National Academy of Sciences, 2022, doi:<a href=\"https://doi.org/10.1073/pnas.2122460119\">10.1073/pnas.2122460119</a>.","ieee":"R. Abualia <i>et al.</i>, “Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 119, no. 31. National Academy of Sciences, 2022.","ama":"Abualia R, Ötvös K, Novák O, et al. Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2022;119(31). doi:<a href=\"https://doi.org/10.1073/pnas.2122460119\">10.1073/pnas.2122460119</a>","chicago":"Abualia, Rashed, Krisztina Ötvös, Ondřej Novák, Eleonore Bouguyon, Kevin Domanegg, Anne Krapp, Philip Nacry, Alain Gojon, Benoit Lacombe, and Eva Benková. “Molecular Framework Integrating Nitrate Sensing in Root and Auxin-Guided Shoot Adaptive Responses.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2022. <a href=\"https://doi.org/10.1073/pnas.2122460119\">https://doi.org/10.1073/pnas.2122460119</a>.","apa":"Abualia, R., Ötvös, K., Novák, O., Bouguyon, E., Domanegg, K., Krapp, A., … Benková, E. (2022). Molecular framework integrating nitrate sensing in root and auxin-guided shoot adaptive responses. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2122460119\">https://doi.org/10.1073/pnas.2122460119</a>"},"file":[{"success":1,"creator":"dernst","checksum":"6e97dedc281247fc3fe238a209f14af0","access_level":"open_access","date_created":"2022-08-08T07:09:58Z","date_updated":"2022-08-08T07:09:58Z","file_name":"2022_PNAS_Abualia.pdf","content_type":"application/pdf","relation":"main_file","file_id":"11744","file_size":3092330}],"abstract":[{"text":"Mineral nutrition is one of the key environmental factors determining plant development and growth. Nitrate is the major form of macronutrient nitrogen that plants take up from the soil. Fluctuating availability or deficiency of this element severely limits plant growth and negatively affects crop production in the agricultural system. To cope with the heterogeneity of nitrate distribution in soil, plants evolved a complex regulatory mechanism that allows rapid adjustment of physiological and developmental processes to the status of this nutrient. The root, as a major exploitation organ that controls the uptake of nitrate to the plant body, acts as a regulatory hub that, according to nitrate availability, coordinates the growth and development of other plant organs. Here, we identified a regulatory framework, where cytokinin response factors (CRFs) play a central role as a molecular readout of the nitrate status in roots to guide shoot adaptive developmental response. We show that nitrate-driven activation of NLP7, a master regulator of nitrate response in plants, fine tunes biosynthesis of cytokinin in roots and its translocation to shoots where it enhances expression of CRFs. CRFs, through direct transcriptional regulation of PIN auxin transporters, promote the flow of auxin and thereby stimulate the development of shoot organs.","lang":"eng"}],"project":[{"_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF","grant_number":"I 1774-B16"}],"publisher":"National Academy of Sciences","year":"2022","article_processing_charge":"No","month":"07","file_date_updated":"2022-08-08T07:09:58Z","type":"journal_article","publication_status":"published","status":"public","date_published":"2022-07-25T00:00:00Z","publication_identifier":{"eissn":["1091-6490"]},"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"has_accepted_license":"1","day":"25","fulldoi":"https://doi.org/10.1073/pnas.2122460119","corr_author":"1","article_type":"original","department":[{"_id":"EvBe"}],"author":[{"first_name":"Rashed","orcid":"0000-0002-9357-9415","id":"4827E134-F248-11E8-B48F-1D18A9856A87","full_name":"Abualia, Rashed","last_name":"Abualia"},{"full_name":"Ötvös, Krisztina","last_name":"Ötvös","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","orcid":"0000-0002-5503-4983"},{"first_name":"Ondřej","full_name":"Novák, Ondřej","last_name":"Novák"},{"first_name":"Eleonore","full_name":"Bouguyon, Eleonore","last_name":"Bouguyon"},{"last_name":"Domanegg","full_name":"Domanegg, Kevin","first_name":"Kevin","orcid":"0000-0002-1215-4264","id":"a24c7829-16e8-11ed-8527-c4d36ffb7539"},{"last_name":"Krapp","full_name":"Krapp, Anne","first_name":"Anne"},{"first_name":"Philip","full_name":"Nacry, Philip","last_name":"Nacry"},{"full_name":"Gojon, Alain","last_name":"Gojon","first_name":"Alain"},{"first_name":"Benoit","last_name":"Lacombe","full_name":"Lacombe, Benoit"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva","last_name":"Benková","full_name":"Benková, Eva"}]},{"article_type":"original","author":[{"full_name":"Marconi, Marco","last_name":"Marconi","first_name":"Marco"},{"full_name":"Gallemi, Marçal","last_name":"Gallemi","id":"460C6802-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4675-6893","first_name":"Marçal"},{"full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Krzysztof","full_name":"Wabnik, Krzysztof","last_name":"Wabnik"}],"department":[{"_id":"EvBe"}],"fulldoi":"https://doi.org/10.7554/elife.72132","day":"01","has_accepted_license":"1","publication_identifier":{"issn":["2050-084X"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa_version":"Published Version","publication_status":"published","status":"public","date_published":"2021-11-01T00:00:00Z","file_date_updated":"2022-05-13T09:00:29Z","type":"journal_article","month":"11","abstract":[{"text":"Plants develop new organs to adjust their bodies to dynamic changes in the environment. How independent organs achieve anisotropic shapes and polarities is poorly understood. To address this question, we constructed a mechano-biochemical model for Arabidopsis root meristem growth that integrates biologically plausible principles. Computer model simulations demonstrate how differential growth of neighboring tissues results in the initial symmetry-breaking leading to anisotropic root growth. Furthermore, the root growth feeds back on a polar transport network of the growth regulator auxin. Model, predictions are in close agreement with in vivo patterns of anisotropic growth, auxin distribution, and cell polarity, as well as several root phenotypes caused by chemical, mechanical, or genetic perturbations. Our study demonstrates that the combination of tissue mechanics and polar auxin transport organizes anisotropic root growth and cell polarities during organ outgrowth. Therefore, a mobile auxin signal transported through immobile cells drives polarity and growth mechanics to coordinate complex organ development.","lang":"eng"}],"year":"2021","article_processing_charge":"Yes","publisher":"eLife Sciences Publications","publication":"eLife","citation":{"mla":"Marconi, Marco, et al. “A Coupled Mechano-Biochemical Model for Cell Polarity Guided Anisotropic Root Growth.” <i>ELife</i>, vol. 10, 72132, eLife Sciences Publications, 2021, doi:<a href=\"https://doi.org/10.7554/elife.72132\">10.7554/elife.72132</a>.","ieee":"M. Marconi, M. Gallemi, E. Benková, and K. Wabnik, “A coupled mechano-biochemical model for cell polarity guided anisotropic root growth,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021.","ista":"Marconi M, Gallemi M, Benková E, Wabnik K. 2021. A coupled mechano-biochemical model for cell polarity guided anisotropic root growth. eLife. 10, 72132.","short":"M. Marconi, M. Gallemi, E. Benková, K. Wabnik, ELife 10 (2021).","apa":"Marconi, M., Gallemi, M., Benková, E., &#38; Wabnik, K. (2021). A coupled mechano-biochemical model for cell polarity guided anisotropic root growth. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.72132\">https://doi.org/10.7554/elife.72132</a>","chicago":"Marconi, Marco, Marçal Gallemi, Eva Benková, and Krzysztof Wabnik. “A Coupled Mechano-Biochemical Model for Cell Polarity Guided Anisotropic Root Growth.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href=\"https://doi.org/10.7554/elife.72132\">https://doi.org/10.7554/elife.72132</a>.","ama":"Marconi M, Gallemi M, Benková E, Wabnik K. A coupled mechano-biochemical model for cell polarity guided anisotropic root growth. <i>eLife</i>. 2021;10. doi:<a href=\"https://doi.org/10.7554/elife.72132\">10.7554/elife.72132</a>"},"file":[{"checksum":"fad13c509b53bb7a2bef9c946a7ca60a","creator":"dernst","success":1,"relation":"main_file","content_type":"application/pdf","file_name":"2021_eLife_Marconi.pdf","file_id":"11372","file_size":14137503,"date_updated":"2022-05-13T09:00:29Z","access_level":"open_access","date_created":"2022-05-13T09:00:29Z"}],"volume":10,"_id":"10270","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa":1,"title":"A coupled mechano-biochemical model for cell polarity guided anisotropic root growth","scopus_import":"1","date_updated":"2023-08-14T11:49:23Z","ddc":["570"],"intvolume":"        10","isi":1,"language":[{"iso":"eng"}],"doi":"10.7554/elife.72132","date_created":"2021-11-11T10:05:18Z","acknowledgement":"e are grateful Richard Smith, Anne-Lise Routier, Crisanto Gutierrez and Juergen Kleine-Vehn for providing critical comments on the manuscript. Funding: This work was supported by the Programa de Atraccion de Talento 2017 (Comunidad de Madrid, 2017-T1/BIO-5654 to KW), Severo Ochoa (SO) Programme for Centres of Excellence in R&D from the Agencia Estatal de Investigacion of Spain (grant SEV-2016–0672 (2017–2021) to KW via the CBGP). In the frame of SEV-2016–0672 funding MM is supported with a postdoctoral contract. KW was supported by Programa Estatal de Generacion del Conocimiento y Fortalecimiento Cientıfico y Tecnologico del Sistema de I + D + I 2019 (PGC2018-093387-A-I00) from MICIU (to KW). MG is recipient of an IST Interdisciplinary Project (IC1022IPC03).","quality_controlled":"1","pmid":1,"external_id":{"pmid":["34723798"],"isi":["000734671200001"]},"article_number":"72132"},{"_id":"9212","volume":13,"citation":{"mla":"Cavallari, Nicola, et al. “Auxin-Regulated Lateral Root Organogenesis.” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 13, no. 7, a039941, Cold Spring Harbor Laboratory Press, 2021, doi:<a href=\"https://doi.org/10.1101/cshperspect.a039941\">10.1101/cshperspect.a039941</a>.","ieee":"N. Cavallari, C. Artner, and E. Benková, “Auxin-regulated lateral root organogenesis,” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 13, no. 7. Cold Spring Harbor Laboratory Press, 2021.","ista":"Cavallari N, Artner C, Benková E. 2021. Auxin-regulated lateral root organogenesis. Cold Spring Harbor Perspectives in Biology. 13(7), a039941.","short":"N. Cavallari, C. Artner, E. Benková, Cold Spring Harbor Perspectives in Biology 13 (2021).","apa":"Cavallari, N., Artner, C., &#38; Benková, E. (2021). Auxin-regulated lateral root organogenesis. <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press. <a href=\"https://doi.org/10.1101/cshperspect.a039941\">https://doi.org/10.1101/cshperspect.a039941</a>","chicago":"Cavallari, Nicola, Christina Artner, and Eva Benková. “Auxin-Regulated Lateral Root Organogenesis.” <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press, 2021. <a href=\"https://doi.org/10.1101/cshperspect.a039941\">https://doi.org/10.1101/cshperspect.a039941</a>.","ama":"Cavallari N, Artner C, Benková E. Auxin-regulated lateral root organogenesis. <i>Cold Spring Harbor Perspectives in Biology</i>. 2021;13(7). doi:<a href=\"https://doi.org/10.1101/cshperspect.a039941\">10.1101/cshperspect.a039941</a>"},"publication":"Cold Spring Harbor Perspectives in Biology","scopus_import":"1","date_updated":"2026-06-18T19:41:34Z","title":"Auxin-regulated lateral root organogenesis","oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/cshperspect.a039941"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1101/cshperspect.a039941","language":[{"iso":"eng"}],"intvolume":"        13","isi":1,"ddc":["580"],"issue":"7","article_number":"a039941","pmid":1,"external_id":{"isi":["000692069100001"],"pmid":["33558367"]},"quality_controlled":"1","acknowledgement":"We apologize to all the authors whose scientific work could not be cited and discussed because of space restrictions. We thank Dr. Inge Verstraeten (ISTAustria) and Dr. Juan Carlos Montesinos-Lopez (ETH Zürich) for helpful suggestions. This work was supported by the DOC Fellowship Programme of the Austrian Academy of Sciences (25008) to C.A.","date_created":"2021-03-01T10:08:32Z","corr_author":"1","fulldoi":"https://doi.org/10.1101/cshperspect.a039941","department":[{"_id":"EvBe"}],"author":[{"last_name":"Cavallari","full_name":"Cavallari, Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","first_name":"Nicola"},{"last_name":"Artner","full_name":"Artner, Christina","first_name":"Christina","id":"45DF286A-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková"}],"article_type":"original","oa_version":"Published Version","publication_identifier":{"issn":["1943-0264"]},"day":"01","type":"journal_article","date_published":"2021-07-01T00:00:00Z","status":"public","publication_status":"published","year":"2021","article_processing_charge":"No","publisher":"Cold Spring Harbor Laboratory Press","project":[{"_id":"2685A872-B435-11E9-9278-68D0E5697425","name":"Hormonal regulation of plant adaptive responses to environmental signals"}],"abstract":[{"lang":"eng","text":"Plant fitness is largely dependent on the root, the underground organ, which, besides its anchoring function, supplies the plant body with water and all nutrients necessary for growth and development. To exploit the soil effectively, roots must constantly integrate environmental signals and react through adjustment of growth and development. Important components of the root management strategy involve a rapid modulation of the root growth kinetics and growth direction, as well as an increase of the root system radius through formation of lateral roots (LRs). At the molecular level, such a fascinating growth and developmental flexibility of root organ requires regulatory networks that guarantee stability of the developmental program but also allows integration of various environmental inputs. The plant hormone auxin is one of the principal endogenous regulators of root system architecture by controlling primary root growth and formation of LR. In this review, we discuss recent progress in understanding molecular networks where auxin is one of the main players shaping the root system and acting as mediator between endogenous cues and environmental factors."}],"month":"07"},{"intvolume":"        22","isi":1,"ddc":["570"],"doi":"10.3390/ijms22083862","language":[{"iso":"eng"}],"quality_controlled":"1","acknowledgement":"This research was supported by a postdoctoral fellowship of the Carl Tryggers Foundation (to K.Ö.) and by grants from Vetenskapsrådet (Nr.: 621-2004-2921 to L.B.) and VINNOVA (to L.B. and S.R.).\r\nWe thank Frederic Berger, Hidehiro Fukaki, Malcolm Bennett, Claudia Köhler, Jiri Friml for providing pRBR1::RBR1-RFP, ssl2-1, slr-1, pPKL::PKL-GFP seeds and the DR5 expressing vector, respectively. Authors are grateful to Hayashi Kenichiro for providing the auxinol compound and to Rishi Bhalerao for stimulating discussions. The technical help of Adeline Rigal and Thomas Vain with the auxinol experiments is much appreciated.","date_created":"2021-04-18T22:01:41Z","issue":"8","article_number":"3862","pmid":1,"external_id":{"pmid":["33917959"],"isi":["000644394800001"]},"citation":{"apa":"Ötvös, K., Miskolczi, P., Marhavý, P., Cruz-Ramírez, A., Benková, E., Robert, S., &#38; Bakó, L. (2021). Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms22083862\">https://doi.org/10.3390/ijms22083862</a>","chicago":"Ötvös, Krisztina, Pál Miskolczi, Peter Marhavý, Alfredo Cruz-Ramírez, Eva Benková, Stéphanie Robert, and László Bakó. “Pickle Recruits Retinoblastoma Related 1 to Control Lateral Root Formation in Arabidopsis.” <i>International Journal of Molecular Sciences</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/ijms22083862\">https://doi.org/10.3390/ijms22083862</a>.","ama":"Ötvös K, Miskolczi P, Marhavý P, et al. Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. <i>International Journal of Molecular Sciences</i>. 2021;22(8). doi:<a href=\"https://doi.org/10.3390/ijms22083862\">10.3390/ijms22083862</a>","ieee":"K. Ötvös <i>et al.</i>, “Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis,” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 8. MDPI, 2021.","mla":"Ötvös, Krisztina, et al. “Pickle Recruits Retinoblastoma Related 1 to Control Lateral Root Formation in Arabidopsis.” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 8, 3862, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/ijms22083862\">10.3390/ijms22083862</a>.","ista":"Ötvös K, Miskolczi P, Marhavý P, Cruz-Ramírez A, Benková E, Robert S, Bakó L. 2021. Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis. International Journal of Molecular Sciences. 22(8), 3862.","short":"K. Ötvös, P. Miskolczi, P. Marhavý, A. Cruz-Ramírez, E. Benková, S. Robert, L. Bakó, International Journal of Molecular Sciences 22 (2021)."},"file":[{"date_updated":"2021-04-19T10:54:55Z","file_size":2769717,"file_id":"9342","content_type":"application/pdf","relation":"main_file","file_name":"2021_JourMolecularScience_Oetvoes.pdf","date_created":"2021-04-19T10:54:55Z","access_level":"open_access","creator":"dernst","checksum":"26ada2531ad1f9c01a1664de0431f1fe","success":1}],"publication":"International Journal of Molecular Sciences","volume":22,"_id":"9332","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","date_updated":"2025-06-12T06:39:41Z","oa":1,"title":"Pickle recruits retinoblastoma related 1 to control lateral root formation in arabidopsis","date_published":"2021-04-08T00:00:00Z","status":"public","publication_status":"published","type":"journal_article","file_date_updated":"2021-04-19T10:54:55Z","month":"04","article_processing_charge":"No","year":"2021","publisher":"MDPI","abstract":[{"text":"Lateral root (LR) formation is an example of a plant post-embryonic organogenesis event. LRs are issued from non-dividing cells entering consecutive steps of formative divisions, proliferation and elongation. The chromatin remodeling protein PICKLE (PKL) negatively regulates auxin-mediated LR formation through a mechanism that is not yet known. Here we show that PKL interacts with RETINOBLASTOMA-RELATED 1 (RBR1) to repress the LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) promoter activity. Since LBD16 function is required for the formative division of LR founder cells, repression mediated by the PKL–RBR1 complex negatively regulates formative division and LR formation. Inhibition of LR formation by PKL–RBR1 is counteracted by auxin, indicating that, in addition to auxin-mediated transcriptional responses, the fine-tuned process of LR formation is also controlled at the chromatin level in an auxin-signaling dependent manner.","lang":"eng"}],"department":[{"_id":"EvBe"}],"author":[{"last_name":"Ötvös","full_name":"Ötvös, Krisztina","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","orcid":"0000-0002-5503-4983"},{"last_name":"Miskolczi","full_name":"Miskolczi, Pál","first_name":"Pál"},{"full_name":"Marhavý, Peter","last_name":"Marhavý","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","first_name":"Peter","orcid":"0000-0001-5227-5741"},{"full_name":"Cruz-Ramírez, Alfredo","last_name":"Cruz-Ramírez","first_name":"Alfredo"},{"full_name":"Benková, Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Robert, Stéphanie","last_name":"Robert","first_name":"Stéphanie"},{"full_name":"Bakó, László","last_name":"Bakó","first_name":"László"}],"article_type":"original","fulldoi":"https://doi.org/10.3390/ijms22083862","day":"08","has_accepted_license":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa_version":"Published Version","publication_identifier":{"issn":["1661-6596"],"eissn":["1422-0067"]}},{"language":[{"iso":"eng"}],"doi":"10.3390/ijms22179222","ddc":["575"],"isi":1,"intvolume":"        22","external_id":{"isi":["000694347100001"],"pmid":["34502129"]},"pmid":1,"issue":"17","article_number":"9222","keyword":["auxin","growth","cell wall","xyloglucans","hypocotyls","gravitropism"],"acknowledgement":"We are grateful to Paul Knox, Markus Pauly, Malcom O’Neill, and Ignacio Zarra for providing published material; the BOKU-VIBT Imaging Center for access and M. Debreczeny for expertise; J.I. Thaker and Georg Seifert for critical reading.\r\n","date_created":"2021-09-05T22:01:24Z","quality_controlled":"1","_id":"9986","volume":22,"publication":"International Journal of Molecular Sciences","citation":{"ista":"Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA, Darino M, Pӗnčík A, Novák O, Kalyna M, Mouille G, Benková E, Bhalerao RP, Mravec J, Kleine-Vehn J. 2021. Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. International Journal of Molecular Sciences. 22(17), 9222.","short":"S.M. Velasquez, X. Guo, M. Gallemi, B. Aryal, P. Venhuizen, E. Barbez, K.A. Dünser, M. Darino, A. Pӗnčík, O. Novák, M. Kalyna, G. Mouille, E. Benková, R.P. Bhalerao, J. Mravec, J. Kleine-Vehn, International Journal of Molecular Sciences 22 (2021).","ieee":"S. M. Velasquez <i>et al.</i>, “Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants,” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 17. MDPI, 2021.","mla":"Velasquez, Silvia Melina, et al. “Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal of Molecular Sciences</i>, vol. 22, no. 17, 9222, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/ijms22179222\">10.3390/ijms22179222</a>.","chicago":"Velasquez, Silvia Melina, Xiaoyuan Guo, Marçal Gallemi, Bibek Aryal, Peter Venhuizen, Elke Barbez, Kai Alexander Dünser, et al. “Xyloglucan Remodeling Defines Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal of Molecular Sciences</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/ijms22179222\">https://doi.org/10.3390/ijms22179222</a>.","ama":"Velasquez SM, Guo X, Gallemi M, et al. Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. <i>International Journal of Molecular Sciences</i>. 2021;22(17). doi:<a href=\"https://doi.org/10.3390/ijms22179222\">10.3390/ijms22179222</a>","apa":"Velasquez, S. M., Guo, X., Gallemi, M., Aryal, B., Venhuizen, P., Barbez, E., … Kleine-Vehn, J. (2021). Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms22179222\">https://doi.org/10.3390/ijms22179222</a>"},"file":[{"creator":"cchlebak","checksum":"6b7055cf89f1b7ed8594c3fdf56f000b","date_updated":"2021-09-07T09:04:53Z","file_name":"2021_IntJMolecularSciences_Velasquez.pdf","content_type":"application/pdf","relation":"main_file","file_size":2162247,"file_id":"9988","access_level":"open_access","date_created":"2021-09-06T12:50:19Z"}],"title":"Xyloglucan remodeling defines auxin-dependent differential tissue expansion in plants","oa":1,"date_updated":"2024-10-09T21:00:50Z","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2021-09-07T09:04:53Z","type":"journal_article","publication_status":"published","status":"public","date_published":"2021-08-26T00:00:00Z","abstract":[{"text":"Size control is a fundamental question in biology, showing incremental complexity in plants, whose cells possess a rigid cell wall. The phytohormone auxin is a vital growth regulator with central importance for differential growth control. Our results indicate that auxin-reliant growth programs affect the molecular complexity of xyloglucans, the major type of cell wall hemicellulose in eudicots. Auxin-dependent induction and repression of growth coincide with reduced and enhanced molecular complexity of xyloglucans, respectively. In agreement with a proposed function in growth control, genetic interference with xyloglucan side decorations distinctly modulates auxin-dependent differential growth rates. Our work proposes that auxin-dependent growth programs have a spatially defined effect on xyloglucan’s molecular structure, which in turn affects cell wall mechanics and specifies differential, gravitropic hypocotyl growth.","lang":"eng"}],"publisher":"MDPI","article_processing_charge":"Yes","year":"2021","month":"08","fulldoi":"https://doi.org/10.3390/ijms22179222","corr_author":"1","article_type":"original","author":[{"last_name":"Velasquez","full_name":"Velasquez, Silvia Melina","first_name":"Silvia Melina"},{"last_name":"Guo","full_name":"Guo, Xiaoyuan","first_name":"Xiaoyuan"},{"id":"460C6802-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4675-6893","first_name":"Marçal","last_name":"Gallemi","full_name":"Gallemi, Marçal"},{"full_name":"Aryal, Bibek","last_name":"Aryal","first_name":"Bibek"},{"first_name":"Peter","full_name":"Venhuizen, Peter","last_name":"Venhuizen"},{"first_name":"Elke","last_name":"Barbez","full_name":"Barbez, Elke"},{"first_name":"Kai Alexander","last_name":"Dünser","full_name":"Dünser, Kai Alexander"},{"first_name":"Martin","last_name":"Darino","full_name":"Darino, Martin"},{"last_name":"Pӗnčík","full_name":"Pӗnčík, Aleš","first_name":"Aleš"},{"full_name":"Novák, Ondřej","last_name":"Novák","first_name":"Ondřej"},{"last_name":"Kalyna","full_name":"Kalyna, Maria","first_name":"Maria"},{"first_name":"Gregory","last_name":"Mouille","full_name":"Mouille, Gregory"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"full_name":"Bhalerao, Rishikesh P.","last_name":"Bhalerao","first_name":"Rishikesh P."},{"last_name":"Mravec","full_name":"Mravec, Jozef","first_name":"Jozef"},{"first_name":"Jürgen","last_name":"Kleine-Vehn","full_name":"Kleine-Vehn, Jürgen"}],"department":[{"_id":"EvBe"}],"publication_identifier":{"issn":["1661-6596"],"eissn":["1422-0067"]},"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"day":"26","has_accepted_license":"1"},{"external_id":{"pmid":[" 33399250"],"isi":["000604645600001"]},"pmid":1,"article_number":"e106862","issue":"3","acknowledgement":"We acknowledge Gergely Molnar for critical reading of the manuscript, Alexander Johnson for language editing and Yulija Salanenka for technical assistance. Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S) to KO, RA and EB. Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S) to KO, RA and EB and by the DOC Fellowship Programme of the AustrianAcademy of Sciences (25008) to C.A. Work in the Wabnik laboratory was supported by the Programa de Atraccion de Talento 2017 (Comunidad deMadrid, 2017-T1/BIO-5654 to K.W.), Severo Ochoa Programme for Centres of Excellence in R&D from the Agencia Estatal de Investigacion of Spain (grantSEV-2016-0672 (2017-2021) to K.W. via the CBGP) and Programa Estatal de Generacion del Conocimiento y Fortalecimiento Científico y Tecnologico del Sistema de I+D+I 2019 (PGC2018-093387-A-I00) from MICIU (to K.W.). M.M.was supported by a postdoctoral contract associated to SEV-2016-0672.We acknowledge the Bioimaging Facility in IST-Austria and the Advanced Microscopy Facility of the Vienna Bio Center Core Facilities, member of the Vienna Bio Center Austria, for use of the OMX v43D SIM microscope. AJ was supported by the Austrian Science Fund (FWF): I03630 to J.F","date_created":"2021-01-17T23:01:12Z","quality_controlled":"1","language":[{"iso":"eng"}],"doi":"10.15252/embj.2020106862","ddc":["580"],"isi":1,"intvolume":"        40","oa":1,"title":"Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport","date_updated":"2026-10-15T22:30:51Z","scopus_import":"1","acknowledged_ssus":[{"_id":"Bio"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","volume":40,"_id":"9010","publication":"EMBO Journal","citation":{"ista":"Ötvös K, Marconi M, Vega A, O’Brien J, Johnson AJ, Abualia R, Antonielli L, Montesinos López JC, Zhang Y, Tan S, Cuesta C, Artner C, Bouguyon E, Gojon A, Friml J, Gutiérrez RA, Wabnik KT, Benková E. 2021. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. EMBO Journal. 40(3), e106862.","short":"K. Ötvös, M. Marconi, A. Vega, J. O’Brien, A.J. Johnson, R. Abualia, L. Antonielli, J.C. Montesinos López, Y. Zhang, S. Tan, C. Cuesta, C. Artner, E. Bouguyon, A. Gojon, J. Friml, R.A. Gutiérrez, K.T. Wabnik, E. Benková, EMBO Journal 40 (2021).","mla":"Ötvös, Krisztina, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” <i>EMBO Journal</i>, vol. 40, no. 3, e106862, Embo Press, 2021, doi:<a href=\"https://doi.org/10.15252/embj.2020106862\">10.15252/embj.2020106862</a>.","ieee":"K. Ötvös <i>et al.</i>, “Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport,” <i>EMBO Journal</i>, vol. 40, no. 3. Embo Press, 2021.","chicago":"Ötvös, Krisztina, Marco Marconi, Andrea Vega, Jose O’Brien, Alexander J Johnson, Rashed Abualia, Livio Antonielli, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” <i>EMBO Journal</i>. Embo Press, 2021. <a href=\"https://doi.org/10.15252/embj.2020106862\">https://doi.org/10.15252/embj.2020106862</a>.","ama":"Ötvös K, Marconi M, Vega A, et al. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. <i>EMBO Journal</i>. 2021;40(3). doi:<a href=\"https://doi.org/10.15252/embj.2020106862\">10.15252/embj.2020106862</a>","apa":"Ötvös, K., Marconi, M., Vega, A., O’Brien, J., Johnson, A. J., Abualia, R., … Benková, E. (2021). Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2020106862\">https://doi.org/10.15252/embj.2020106862</a>"},"file":[{"checksum":"dc55c900f3b061d6c2790b8813d759a3","creator":"dernst","success":1,"file_name":"2021_Embo_Otvos.pdf","content_type":"application/pdf","relation":"main_file","file_id":"9110","file_size":2358617,"date_updated":"2021-02-11T12:28:29Z","access_level":"open_access","date_created":"2021-02-11T12:28:29Z"}],"abstract":[{"text":"Availability of the essential macronutrient nitrogen in soil plays a critical role in plant growth, development, and impacts agricultural productivity. Plants have evolved different strategies for sensing and responding to heterogeneous nitrogen distribution. Modulation of root system architecture, including primary root growth and branching, is among the most essential plant adaptions to ensure adequate nitrogen acquisition. However, the immediate molecular pathways coordinating the adjustment of root growth in response to distinct nitrogen sources, such as nitrate or ammonium, are poorly understood. Here, we show that growth as manifested by cell division and elongation is synchronized by coordinated auxin flux between two adjacent outer tissue layers of the root. This coordination is achieved by nitrate‐dependent dephosphorylation of the PIN2 auxin efflux carrier at a previously uncharacterized phosphorylation site, leading to subsequent PIN2 lateralization and thereby regulating auxin flow between adjacent tissues. A dynamic computer model based on our experimental data successfully recapitulates experimental observations. Our study provides mechanistic insights broadening our understanding of root growth mechanisms in dynamic environments.","lang":"eng"}],"project":[{"call_identifier":"FWF","name":"Hormone cross-talk drives nutrient dependent plant development","_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16"},{"_id":"2685A872-B435-11E9-9278-68D0E5697425","name":"Hormonal regulation of plant adaptive responses to environmental signals"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","grant_number":"I03630"}],"publisher":"Embo Press","article_processing_charge":"Yes (via OA deal)","year":"2021","month":"02","file_date_updated":"2021-02-11T12:28:29Z","type":"journal_article","publication_status":"published","status":"public","date_published":"2021-02-01T00:00:00Z","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"day":"01","has_accepted_license":"1","fulldoi":"https://doi.org/10.15252/embj.2020106862","corr_author":"1","article_type":"original","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"author":[{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","orcid":"0000-0002-5503-4983","last_name":"Ötvös","full_name":"Ötvös, Krisztina"},{"first_name":"Marco","last_name":"Marconi","full_name":"Marconi, Marco"},{"last_name":"Vega","full_name":"Vega, Andrea","first_name":"Andrea"},{"first_name":"Jose","full_name":"O’Brien, Jose","last_name":"O’Brien"},{"last_name":"Johnson","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","orcid":"0000-0002-2739-8843"},{"id":"4827E134-F248-11E8-B48F-1D18A9856A87","first_name":"Rashed","orcid":"0000-0002-9357-9415","last_name":"Abualia","full_name":"Abualia, Rashed"},{"first_name":"Livio","last_name":"Antonielli","full_name":"Antonielli, Livio"},{"id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9179-6099","first_name":"Juan C","full_name":"Montesinos López, Juan C","last_name":"Montesinos López"},{"id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956","first_name":"Yuzhou","full_name":"Zhang, Yuzhou","last_name":"Zhang"},{"full_name":"Tan, Shutang","last_name":"Tan","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0471-8285","first_name":"Shutang"},{"id":"33A3C818-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1923-2410","first_name":"Candela","last_name":"Cuesta","full_name":"Cuesta, Candela"},{"full_name":"Artner, Christina","last_name":"Artner","id":"45DF286A-F248-11E8-B48F-1D18A9856A87","first_name":"Christina"},{"first_name":"Eleonore","last_name":"Bouguyon","full_name":"Bouguyon, Eleonore"},{"first_name":"Alain","full_name":"Gojon, Alain","last_name":"Gojon"},{"full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Rodrigo A.","last_name":"Gutiérrez","full_name":"Gutiérrez, Rodrigo A."},{"full_name":"Wabnik, Krzysztof T","last_name":"Wabnik","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7263-0560","first_name":"Krzysztof T"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"}],"related_material":{"link":[{"url":"https://ist.ac.at/en/news/a-plants-way-to-its-favorite-food/","description":"News on IST Homepage","relation":"press_release"}],"record":[{"relation":"dissertation_contains","id":"10303","status":"public"}]}},{"fulldoi":"https://doi.org/10.15252/embr.202051813","related_material":{"record":[{"relation":"dissertation_contains","id":"10303","status":"public"}]},"author":[{"first_name":"Andrea","full_name":"Vega, Andrea","last_name":"Vega"},{"full_name":"Fredes, Isabel","last_name":"Fredes","first_name":"Isabel"},{"first_name":"José","last_name":"O’Brien","full_name":"O’Brien, José"},{"first_name":"Zhouxin","last_name":"Shen","full_name":"Shen, Zhouxin"},{"last_name":"Ötvös","full_name":"Ötvös, Krisztina","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5503-4983","first_name":"Krisztina"},{"full_name":"Abualia, Rashed","last_name":"Abualia","id":"4827E134-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9357-9415","first_name":"Rashed"},{"full_name":"Benková, Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Steven P.","full_name":"Briggs, Steven P.","last_name":"Briggs"},{"first_name":"Rodrigo A.","last_name":"Gutiérrez","full_name":"Gutiérrez, Rodrigo A."}],"department":[{"_id":"EvBe"},{"_id":"GradSch"}],"article_type":"original","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"publication_identifier":{"issn":["1469-221X"],"eissn":["1469-3178"]},"has_accepted_license":"1","day":"06","type":"journal_article","file_date_updated":"2021-10-05T13:36:42Z","date_published":"2021-09-06T00:00:00Z","status":"public","publication_status":"published","year":"2021","article_processing_charge":"Yes","publisher":"Wiley","abstract":[{"lang":"eng","text":"Nitrate commands genome-wide gene expression changes that impact metabolism, physiology, plant growth, and development. In an effort to identify new components involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments via liquid chromatography coupled to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5 or 20 min after nitrate treatments. Proteins identified by 5 min include signaling components such as kinases or transcription factors. In contrast, by 20 min, proteins identified were associated with transporter activity or hormone metabolism functions, among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1) mutant plants was significantly altered as compared to wild-type plants, confirming its key role in nitrate signaling pathways that involves phosphorylation changes. Integrative bioinformatics analysis highlights auxin transport as an important mechanism modulated by nitrate signaling at the post-translational level. We validated a new phosphorylation site in PIN2 and provide evidence that it functions in primary and lateral root growth responses to nitrate."}],"month":"09","_id":"9913","volume":22,"citation":{"ieee":"A. Vega <i>et al.</i>, “Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture,” <i>EMBO Reports</i>, vol. 22, no. 9. Wiley, 2021.","mla":"Vega, Andrea, et al. “Nitrate Triggered Phosphoproteome Changes and a PIN2 Phosphosite Modulating Root System Architecture.” <i>EMBO Reports</i>, vol. 22, no. 9, e51813, Wiley, 2021, doi:<a href=\"https://doi.org/10.15252/embr.202051813\">10.15252/embr.202051813</a>.","ista":"Vega A, Fredes I, O’Brien J, Shen Z, Ötvös K, Abualia R, Benková E, Briggs SP, Gutiérrez RA. 2021. Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. EMBO Reports. 22(9), e51813.","short":"A. Vega, I. Fredes, J. O’Brien, Z. Shen, K. Ötvös, R. Abualia, E. Benková, S.P. Briggs, R.A. Gutiérrez, EMBO Reports 22 (2021).","apa":"Vega, A., Fredes, I., O’Brien, J., Shen, Z., Ötvös, K., Abualia, R., … Gutiérrez, R. A. (2021). Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. <i>EMBO Reports</i>. Wiley. <a href=\"https://doi.org/10.15252/embr.202051813\">https://doi.org/10.15252/embr.202051813</a>","chicago":"Vega, Andrea, Isabel Fredes, José O’Brien, Zhouxin Shen, Krisztina Ötvös, Rashed Abualia, Eva Benková, Steven P. Briggs, and Rodrigo A. Gutiérrez. “Nitrate Triggered Phosphoproteome Changes and a PIN2 Phosphosite Modulating Root System Architecture.” <i>EMBO Reports</i>. Wiley, 2021. <a href=\"https://doi.org/10.15252/embr.202051813\">https://doi.org/10.15252/embr.202051813</a>.","ama":"Vega A, Fredes I, O’Brien J, et al. Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. <i>EMBO Reports</i>. 2021;22(9). doi:<a href=\"https://doi.org/10.15252/embr.202051813\">10.15252/embr.202051813</a>"},"file":[{"access_level":"open_access","date_created":"2021-10-05T13:36:42Z","relation":"main_file","file_name":"2021_EmboR_Vega.pdf","content_type":"application/pdf","file_size":3144854,"file_id":"10090","date_updated":"2021-10-05T13:36:42Z","success":1,"checksum":"750de03dc3b715c37090126c1548ba13","creator":"cchlebak"}],"publication":"EMBO Reports","scopus_import":"1","date_updated":"2026-10-15T22:30:51Z","oa":1,"title":"Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.15252/embr.202051813","language":[{"iso":"eng"}],"intvolume":"        22","isi":1,"ddc":["580"],"article_number":"e51813","issue":"9","pmid":1,"external_id":{"pmid":["34357701 "],"isi":["000681754200001"]},"quality_controlled":"1","acknowledgement":"This work was supported by ANID—Millennium Science Initiative Program—ICN17_022, Fondo de Desarrollo de Areas Prioritarias (FONDAP) Center for Genome Regulation (15090007), ANID—Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) 1180759 (to RAG) and 1171631 (to AV). We would like to thank Unidad de Microscopía Avanzada UC (UMA UC).","date_created":"2021-08-15T22:01:30Z"},{"language":[{"iso":"eng"}],"doi":"10.1038/s41467-020-15895-5","ddc":["570"],"intvolume":"        11","isi":1,"pmid":1,"external_id":{"pmid":["32358503"],"isi":["000531425900012"]},"article_number":"2170","acknowledgement":"We thank Daria Siekhaus, Jiri Friml and Alexander Johnson for critical reading of the manuscript, Peter Pimpl, Christian Luschnig and Liwen Jiang for sharing published material, Lesia Rodriguez Solovey for technical assistance. This work was supported by the Austrian Science Fund (FWF01_I1774S) to A.H., K.Ö., and E.B., the German Research Foundation (DFG; He3424/6-1 to I.H.), by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n° [291734] (to N.C.), by the EU in the framework of the Marie-Curie FP7 COFUND People Programme through the award of an AgreenSkills+ fellowship No. 609398 (to J.S.) and by the Scientific Service Units of IST-Austria through resources provided by the Bioimaging Facility, the Life Science Facility. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","date_created":"2020-05-10T22:00:48Z","quality_controlled":"1","volume":11,"_id":"7805","publication":"Nature Communications","citation":{"mla":"Hurny, Andrej, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>, vol. 11, 2170, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>.","ieee":"A. Hurny <i>et al.</i>, “Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","ista":"Hurny A, Cuesta C, Cavallari N, Ötvös K, Duclercq J, Dokládal L, Montesinos López JC, Gallemi M, Semerádová H, Rauter T, Stenzel I, Persiau G, Benade F, Bhalearo R, Sýkorová E, Gorzsás A, Sechet J, Mouille G, Heilmann I, De Jaeger G, Ludwig-Müller J, Benková E. 2020. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. Nature Communications. 11, 2170.","short":"A. Hurny, C. Cuesta, N. Cavallari, K. Ötvös, J. Duclercq, L. Dokládal, J.C. Montesinos López, M. Gallemi, H. Semerádová, T. Rauter, I. Stenzel, G. Persiau, F. Benade, R. Bhalearo, E. Sýkorová, A. Gorzsás, J. Sechet, G. Mouille, I. Heilmann, G. De Jaeger, J. Ludwig-Müller, E. Benková, Nature Communications 11 (2020).","apa":"Hurny, A., Cuesta, C., Cavallari, N., Ötvös, K., Duclercq, J., Dokládal, L., … Benková, E. (2020). Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>","chicago":"Hurny, Andrej, Candela Cuesta, Nicola Cavallari, Krisztina Ötvös, Jerome Duclercq, Ladislav Dokládal, Juan C Montesinos López, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>.","ama":"Hurny A, Cuesta C, Cavallari N, et al. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>"},"file":[{"success":1,"creator":"dernst","checksum":"2cba327c9e9416d75cb96be54b0fb441","date_created":"2020-10-06T07:47:53Z","access_level":"open_access","date_updated":"2020-10-06T07:47:53Z","file_id":"8614","file_size":4743576,"content_type":"application/pdf","relation":"main_file","file_name":"2020_NatureComm_Hurny.pdf"}],"title":"Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance","oa":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"scopus_import":"1","date_updated":"2026-04-02T14:32:53Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","file_date_updated":"2020-10-06T07:47:53Z","type":"journal_article","publication_status":"published","status":"public","date_published":"2020-05-01T00:00:00Z","project":[{"_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"}],"abstract":[{"lang":"eng","text":"Plants as non-mobile organisms constantly integrate varying environmental signals to flexibly adapt their growth and development. Local fluctuations in water and nutrient availability, sudden changes in temperature or other abiotic and biotic stresses can trigger changes in the growth of plant organs. Multiple mutually interconnected hormonal signaling cascades act as essential endogenous translators of these exogenous signals in the adaptive responses of plants. Although the molecular backbones of hormone transduction pathways have been identified, the mechanisms underlying their interactions are largely unknown. Here, using genome wide transcriptome profiling we identify an auxin and cytokinin cross-talk component; SYNERGISTIC ON AUXIN AND CYTOKININ 1 (SYAC1), whose expression in roots is strictly dependent on both of these hormonal pathways. We show that SYAC1 is a regulator of secretory pathway, whose enhanced activity interferes with deposition of cell wall components and can fine-tune organ growth and sensitivity to soil pathogens."}],"year":"2020","article_processing_charge":"No","publisher":"Springer Nature","month":"05","ec_funded":1,"corr_author":"1","fulldoi":"https://doi.org/10.1038/s41467-020-15895-5","article_type":"original","author":[{"id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3638-1426","first_name":"Andrej","full_name":"Hurny, Andrej","last_name":"Hurny"},{"last_name":"Cuesta","full_name":"Cuesta, Candela","first_name":"Candela","orcid":"0000-0003-1923-2410","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87","last_name":"Cavallari","full_name":"Cavallari, Nicola"},{"full_name":"Ötvös, Krisztina","last_name":"Ötvös","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5503-4983","first_name":"Krisztina"},{"full_name":"Duclercq, Jerome","last_name":"Duclercq","first_name":"Jerome"},{"last_name":"Dokládal","full_name":"Dokládal, Ladislav","first_name":"Ladislav"},{"full_name":"Montesinos López, Juan C","last_name":"Montesinos López","orcid":"0000-0001-9179-6099","first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Gallemi","full_name":"Gallemi, Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4675-6893","first_name":"Marçal"},{"first_name":"Hana","id":"42FE702E-F248-11E8-B48F-1D18A9856A87","full_name":"Semeradova, Hana","last_name":"Semeradova"},{"full_name":"Rauter, Thomas","last_name":"Rauter","first_name":"Thomas","id":"A0385D1A-9376-11EA-A47D-9862C5E3AB22"},{"first_name":"Irene","full_name":"Stenzel, Irene","last_name":"Stenzel"},{"full_name":"Persiau, Geert","last_name":"Persiau","first_name":"Geert"},{"first_name":"Freia","last_name":"Benade","full_name":"Benade, Freia"},{"first_name":"Rishikesh","full_name":"Bhalearo, Rishikesh","last_name":"Bhalearo"},{"first_name":"Eva","last_name":"Sýkorová","full_name":"Sýkorová, Eva"},{"full_name":"Gorzsás, András","last_name":"Gorzsás","first_name":"András"},{"first_name":"Julien","full_name":"Sechet, Julien","last_name":"Sechet"},{"first_name":"Gregory","full_name":"Mouille, Gregory","last_name":"Mouille"},{"last_name":"Heilmann","full_name":"Heilmann, Ingo","first_name":"Ingo"},{"last_name":"De Jaeger","full_name":"De Jaeger, Geert","first_name":"Geert"},{"first_name":"Jutta","full_name":"Ludwig-Müller, Jutta","last_name":"Ludwig-Müller"},{"last_name":"Benková","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva"}],"department":[{"_id":"EvBe"}],"publication_identifier":{"eissn":["2041-1723"]},"oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","day":"01"}]