---
OA_type: closed access
_id: '22286'
abstract:
- lang: eng
text: Plants are remarkable organisms. Unlike animals, they cannot flee and, rooted
in one place, they must cope with whatever challenges arise — nutrient scarcity,
drought, shade, wind or obstacles in the soil. Their extraordinary ability to
survive in such unstable environmental conditions lies in their capacity to adapt.
In response to environmental signals, plants can rapidly adjust the rate of organ
growth, change the direction of growth, bend toward resources, or remodel their
body architecture by promoting or suppressing the formation of new organs such
as lateral roots, branches, leaves, or flowers. This unique developmental plasticity
depends on chemical signals, plant hormones that serve as regulators and coordinators
of endogenous molecular and cellular processes. Chief among these signals is auxin,
a plant hormone central to nearly every aspect of plant life.
article_processing_charge: No
article_type: original
author:
- first_name: Valentin
full_name: Leitner, Valentin
id: 4c665ce3-0016-11ec-bea0-e44de7a4fa3d
last_name: Leitner
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Leitner V, Benková E. Auxin and the control of plant growth and development.
Current Biology. 2026;36(13):R739-R744. doi:10.1016/j.cub.2026.04.047
apa: Leitner, V., & Benková, E. (2026). Auxin and the control of plant growth
and development. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2026.04.047
chicago: Leitner, Valentin, and Eva Benková. “Auxin and the Control of Plant Growth
and Development.” Current Biology. Elsevier, 2026. https://doi.org/10.1016/j.cub.2026.04.047.
ieee: V. Leitner and E. Benková, “Auxin and the control of plant growth and development,”
Current Biology, vol. 36, no. 13. Elsevier, pp. R739–R744, 2026.
ista: Leitner V, Benková E. 2026. Auxin and the control of plant growth and development.
Current Biology. 36(13), R739–R744.
mla: Leitner, Valentin, and Eva Benková. “Auxin and the Control of Plant Growth
and Development.” Current Biology, vol. 36, no. 13, Elsevier, 2026, pp.
R739–44, doi:10.1016/j.cub.2026.04.047.
short: V. Leitner, E. Benková, Current Biology 36 (2026) R739–R744.
corr_author: '1'
das_tickbox: '0'
date_created: 2026-07-13T09:39:40Z
date_published: 2026-07-06T00:00:00Z
date_updated: 2026-07-13T11:11:56Z
day: '06'
department:
- _id: EvBe
- _id: GradSch
doi: 10.1016/j.cub.2026.04.047
external_id:
pmid:
- '42407441'
fulldoi: https://doi.org/10.1016/j.cub.2026.04.047
intvolume: ' 36'
issue: '13'
language:
- iso: eng
month: '07'
oa_version: None
page: R739-R744
pmid: 1
publication: Current Biology
publication_identifier:
issn:
- 0960-9822
publication_status: published
publisher: Elsevier
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: no
title: Auxin and the control of plant growth and development
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2026'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '22712'
abstract:
- lang: eng
text: Ginseng (Panax ginseng) derives its renowned therapeutic properties from ginsenoside
metabolites. However, the long cultivation cycle and susceptibility to diseases
hinder the advancement of the ginseng industry. Here, we demonstrate that the
embryonic protoderm of ginseng can efficiently produce ginsenosides. Single-cell
transcriptome and mass spectrometry imaging analyses reveal that ginsenosides
accumulate in the protoderm of ginseng embryonic callus (EC) at levels comparable
to those in forest ginseng. Epigenetic analyses indicate that elevated histone
acetylation and enhanced chromatin accessibility at regeneration- and ginsenoside
metabolism-related gene loci are associated with the ginsenoside-producing capacity
of EC. Increasing histone acetylation levels or overexpressing the regeneration-related
WUSCHEL-RELATED HOMEOBOX11 (WOX11) gene further enhances ginsenoside production
in EC. Our findings suggest that the protoderm of EC could serve as an in situ
biological compartment for high-efficiency ginsenoside producion, offering a complementary
approach to traditional ginseng cultivation.
acknowledgement: 'The authors are grateful to Professor Linfeng Li from the School
of Life Science, Fudan University, for his assistance during the ginseng genome
annotation. This work was supported by Key project at central government level:
The ability establishment of sustainable use for valuable Chinese medicine resources
(2060302-2401-08 to L.X. and J.L.), the National Natural Science Foundation of China
(82373987 to J.L., 31701294 to L.X., 32225007 to L.X., and 32300285 to N.Z.), the
Fundamental Research Funds for the Central public welfare research institutes (ZZ13-YQ-093,
ZZXT202508 to J.L.), the CACMS Innovation Fund (CI2025G00-06 to J.L.), the Principle
Investigator Program (HBMUPI202104 to Y.Z.), the Key R&D Program of Shandong Province,
China (2024LZGC025 to L.X.), the National Key R&D Program of China (2024YFF1000700/2023YFE0101100
to L.X.), Strategic Priority Research Program of Chinese Academy of Sciences (XDB0630000
to L.X.), and China Postdoctoral Science Foundation (2023M733490 to N.Z.).'
article_number: '7994'
article_processing_charge: Yes
article_type: original
author:
- first_name: Juan
full_name: Liu, Juan
last_name: Liu
- first_name: Ning
full_name: Zhai, Ning
last_name: Zhai
- first_name: Shiyi
full_name: Zhang, Shiyi
last_name: Zhang
- first_name: Yosuke
full_name: Tamada, Yosuke
last_name: Tamada
- first_name: Tonghui
full_name: Li, Tonghui
last_name: Li
- first_name: Linfan
full_name: Zhang, Linfan
last_name: Zhang
- first_name: Tong
full_name: Chen, Tong
last_name: Chen
- first_name: Chenglin
full_name: Wang, Chenglin
last_name: Wang
- first_name: Jian
full_name: Yang, Jian
last_name: Yang
- first_name: Jiaqi
full_name: Gao, Jiaqi
last_name: Gao
- first_name: Xiang
full_name: Li, Xiang
id: 4B7E523C-F248-11E8-B48F-1D18A9856A87
last_name: Li
- first_name: Junhui
full_name: Zhou, Junhui
last_name: Zhou
- first_name: Yonghong
full_name: Zhang, Yonghong
last_name: Zhang
- first_name: Yu
full_name: Liu, Yu
id: 2A70014E-F248-11E8-B48F-1D18A9856A87
last_name: Liu
orcid: 0000-0001-7313-6740
- first_name: Yuan
full_name: Wang, Yuan
last_name: Wang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Chen
full_name: Li, Chen
last_name: Li
- first_name: Lin
full_name: Xu, Lin
last_name: Xu
- first_name: Luqi
full_name: Huang, Luqi
last_name: Huang
citation:
ama: Liu J, Zhai N, Zhang S, et al. Single-cell analyses identify the ginseng embryonic
protoderm as a native compartment for high-efficiency ginsenoside production.
Nature Communications. 2026;17. doi:10.1038/s41467-026-74881-5
apa: Liu, J., Zhai, N., Zhang, S., Tamada, Y., Li, T., Zhang, L., … Huang, L. (2026).
Single-cell analyses identify the ginseng embryonic protoderm as a native compartment
for high-efficiency ginsenoside production. Nature Communications. Springer
Nature. https://doi.org/10.1038/s41467-026-74881-5
chicago: Liu, Juan, Ning Zhai, Shiyi Zhang, Yosuke Tamada, Tonghui Li, Linfan Zhang,
Tong Chen, et al. “Single-Cell Analyses Identify the Ginseng Embryonic Protoderm
as a Native Compartment for High-Efficiency Ginsenoside Production.” Nature
Communications. Springer Nature, 2026. https://doi.org/10.1038/s41467-026-74881-5.
ieee: J. Liu et al., “Single-cell analyses identify the ginseng embryonic
protoderm as a native compartment for high-efficiency ginsenoside production,”
Nature Communications, vol. 17. Springer Nature, 2026.
ista: Liu J, Zhai N, Zhang S, Tamada Y, Li T, Zhang L, Chen T, Wang C, Yang J, Gao
J, Li X, Zhou J, Zhang Y, Liu Y, Wang Y, Friml J, Benková E, Li C, Xu L, Huang
L. 2026. Single-cell analyses identify the ginseng embryonic protoderm as a native
compartment for high-efficiency ginsenoside production. Nature Communications.
17, 7994.
mla: Liu, Juan, et al. “Single-Cell Analyses Identify the Ginseng Embryonic Protoderm
as a Native Compartment for High-Efficiency Ginsenoside Production.” Nature
Communications, vol. 17, 7994, Springer Nature, 2026, doi:10.1038/s41467-026-74881-5.
short: J. Liu, N. Zhai, S. Zhang, Y. Tamada, T. Li, L. Zhang, T. Chen, C. Wang,
J. Yang, J. Gao, X. Li, J. Zhou, Y. Zhang, Y. Liu, Y. Wang, J. Friml, E. Benková,
C. Li, L. Xu, L. Huang, Nature Communications 17 (2026).
das_tickbox: '1'
dataavailabilitystatement: The RNA-seq data generated in this study have been deposited
in the GSA database under accession code CRA008967. The scRNA-seq data generated
in this study have been deposited in the GSA database under accession code CRA026200.
The ATAC-seq data generated in this study have been deposited in the GSA database
under accession code CRA008969. The ChIP-seq data generated in this study have been
deposited in the GSA database under accession code CRA008968. Single-cell RNA-seq
data and scripts are publicly available on Zenodo (https://zenodo.org/records/20392012).
Primers are in Supplemental Table 5. Source data are provided with this paper.
date_created: 2026-08-16T22:01:42Z
date_published: 2026-08-07T00:00:00Z
date_updated: 2026-08-20T06:45:46Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41467-026-74881-5
external_id:
pmid:
- '42350386'
file:
- access_level: open_access
checksum: 0b4ff29f0d0168b11be32a5f22ded9e4
content_type: application/pdf
creator: dernst
date_created: 2026-08-20T06:44:33Z
date_updated: 2026-08-20T06:44:33Z
file_id: '22742'
file_name: 2026_NatureComm_Liu.pdf
file_size: 2429551
relation: main_file
success: 1
file_date_updated: 2026-08-20T06:44:33Z
fulldoi: https://doi.org/10.1038/s41467-026-74881-5
has_accepted_license: '1'
intvolume: ' 17'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Single-cell analyses identify the ginseng embryonic protoderm as a native compartment
for high-efficiency ginsenoside production
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2026'
...
---
OA_type: closed access
_id: '19594'
abstract:
- lang: eng
text: In this issue of Developmental Cell, Lee et al. identify a pivotal role for
glutathione (GSH) in plant regeneration, a vital biological process enabling plants
to regrow tissues and organs after injury. Applying single-cell RNA sequencing
(scRNA-seq) and live imaging, the authors demonstrate that GSH, released upon
tissue damage, accelerates cell-cycle transitions, particularly shortening the
G1 phase, thereby facilitating efficient organ regeneration.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: 'Benková E. Unlocking plant regeneration: The role for glutathione. Developmental
Cell. 2025;60(8):1137-1139. doi:10.1016/j.devcel.2025.03.012'
apa: 'Benková, E. (2025). Unlocking plant regeneration: The role for glutathione.
Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.03.012'
chicago: 'Benková, Eva. “Unlocking Plant Regeneration: The Role for Glutathione.”
Developmental Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2025.03.012.'
ieee: 'E. Benková, “Unlocking plant regeneration: The role for glutathione,” Developmental
Cell, vol. 60, no. 8. Elsevier, pp. 1137–1139, 2025.'
ista: 'Benková E. 2025. Unlocking plant regeneration: The role for glutathione.
Developmental Cell. 60(8), 1137–1139.'
mla: 'Benková, Eva. “Unlocking Plant Regeneration: The Role for Glutathione.” Developmental
Cell, vol. 60, no. 8, Elsevier, 2025, pp. 1137–39, doi:10.1016/j.devcel.2025.03.012.'
short: E. Benková, Developmental Cell 60 (2025) 1137–1139.
corr_author: '1'
date_created: 2025-04-20T22:01:28Z
date_published: 2025-04-21T00:00:00Z
date_updated: 2025-09-30T12:07:36Z
day: '21'
department:
- _id: EvBe
doi: 10.1016/j.devcel.2025.03.012
external_id:
isi:
- '001477400800001'
pmid:
- '40262524'
fulldoi: https://doi.org/10.1016/j.devcel.2025.03.012
intvolume: ' 60'
isi: 1
issue: '8'
language:
- iso: eng
month: '04'
oa_version: None
page: 1137-1139
pmid: 1
publication: Developmental Cell
publication_identifier:
eissn:
- 1878-1551
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Unlocking plant regeneration: The role for glutathione'
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 60
year: '2025'
...
---
APC_amount: 3642,79 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20080'
abstract:
- lang: eng
text: "Introduction: Acid-growth theory has been postulated in the 70s to explain
the rapid elongation of plant cells in response to the hormone auxin. More recently,
it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting
acidification of the apoplast is the principal mechanism by which auxin and other
hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances,
the impact of this acidification on the mechanical properties of the cell wall
remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of
Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate
hormone-induced tissue elongation and local changes in cell wall mechanical properties.
Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase
(PME), along with calcium chelators, we investigate the effect of pectin modification
in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification
of apoplast is necessary and sufficient to induce cell elongation through promoting
cell wall softening. Moreover, we show that enhanced PME activity can induce both
cell wall softening or stiffening in extracellular calcium dependent-manner and
that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion:
Our results confirm a dual role of PME in plant cell elongation. However, further
investigation is needed to assess the status of pectin following short- or long-term
PME treatments in order to determine if pectin methyl-esterification might promote
its degradation as well as the role of PME inhibitors upon PME induction."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: E-Lib
acknowledgement: "The author(s) declare that financial support was received for the
research and/or publication of this article. This work was supported by grants from
the European Research Council (Starting Independent Research Grant ERC-2007-Stg-
207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe
acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge
CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127).
We gratefully acknowledge support by the Scientific Service Units at ISTA, including
the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler
for the efforts to establish immunodetection method."
article_number: '1612366'
article_processing_charge: Yes
article_type: original
author:
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Nikola
full_name: Zarevski, Nikola
id: 18e95355-e05a-11ea-a9c0-8fba1b89e83a
last_name: Zarevski
- first_name: Jan
full_name: Pribyl, Jan
last_name: Pribyl
- first_name: Petr
full_name: Skládal, Petr
last_name: Skládal
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl
esterase activity in the regulation of plant cell wall biophysical properties.
Frontiers in Plant Science. 2025;16. doi:10.3389/fpls.2025.1612366
apa: Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P.,
Hannezo, E. B., & Benková, E. (2025). Dual role of pectin methyl esterase
activity in the regulation of plant cell wall biophysical properties. Frontiers
in Plant Science. Frontiers Media. https://doi.org/10.3389/fpls.2025.1612366
chicago: Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl,
Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl
Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.”
Frontiers in Plant Science. Frontiers Media, 2025. https://doi.org/10.3389/fpls.2025.1612366.
ieee: M. Gallemi et al., “Dual role of pectin methyl esterase activity in
the regulation of plant cell wall biophysical properties,” Frontiers in Plant
Science, vol. 16. Frontiers Media, 2025.
ista: Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB,
Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation
of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.
mla: Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the
Regulation of Plant Cell Wall Biophysical Properties.” Frontiers in Plant Science,
vol. 16, 1612366, Frontiers Media, 2025, doi:10.3389/fpls.2025.1612366.
short: M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B.
Hannezo, E. Benková, Frontiers in Plant Science 16 (2025).
corr_author: '1'
date_created: 2025-07-27T22:01:26Z
date_published: 2025-07-04T00:00:00Z
date_updated: 2026-05-20T07:53:03Z
day: '04'
ddc:
- '580'
department:
- _id: EdHa
- _id: EvBe
- _id: CaGu
doi: 10.3389/fpls.2025.1612366
ec_funded: 1
external_id:
isi:
- '001530690900001'
pmid:
- '40688689'
file:
- access_level: open_access
checksum: 9e6b8b53ba56d4a24a9bd91cf6d2dc58
content_type: application/pdf
creator: dernst
date_created: 2025-07-31T07:28:54Z
date_updated: 2025-07-31T07:28:54Z
file_id: '20093'
file_name: 2025_FrontiersPlantSc_Gallemi.pdf
file_size: 3665187
relation: main_file
success: 1
file_date_updated: 2025-07-31T07:28:54Z
fulldoi: https://doi.org/10.3389/fpls.2025.1612366
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
- _id: B67AFEDC-15C9-11EA-A837-991A96BB2854
name: IST Austria Open Access Fund
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664-462X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dual role of pectin methyl esterase activity in the regulation of plant cell
wall biophysical properties
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '19003'
abstract:
- lang: eng
text: 'Super-resolution methods provide far better spatial resolution than the optical
diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless,
they have yet to attain widespread use in plants, largely due to plants’ challenging
optical properties. Expansion microscopy improves effective resolution by isotropically
increasing the physical distances between sample structures while preserving relative
spatial arrangements and clearing the sample. However, its application to plants
has been hindered by the rigid, mechanically cohesive structure of plant tissues.
Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis
thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over
conventional microscopy. Our results highlight the microtubule cytoskeleton organization
and interaction between molecularly defined cellular constituents. Combining PlantEx
with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution
and visualize the complex organization of subcellular organelles from intact tissues
by example of the densely packed COPI-coated vesicles associated with the Golgi
apparatus and put these into a cellular structural context. Our results show that
expansion microscopy can be applied to increase effective imaging resolution in
Arabidopsis root specimens. '
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: E-Lib
- _id: M-Shop
acknowledgement: "We gratefully acknowledge support by the Scientific Service Units
at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical
workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis
project has received funding from the European Research Council under the Horizon
2020 Framework Programme (grant agreement No 742985, J.F.). It has also received
funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie
Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow
from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement
no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018).
M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship
no. 26137. The project has further received funding from the Austrian Science Fund,
via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship
from the Human Frontier Science Program (LT000557/2018). The funders had no role
in study design, data collection and analysis, decision to publish or preparation
of the manuscript."
article_number: koaf006
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Sven M
full_name: Truckenbrodt, Sven M
id: 45812BD4-F248-11E8-B48F-1D18A9856A87
last_name: Truckenbrodt
- first_name: Caroline
full_name: Kreuzinger, Caroline
id: 382077BA-F248-11E8-B48F-1D18A9856A87
last_name: Kreuzinger
- first_name: Syamala
full_name: Inumella, Syamala
id: F8660870-D756-11E9-98C5-34DFE5697425
last_name: Inumella
orcid: 0009-0002-5890-120X
- first_name: Vitali
full_name: Vistunou, Vitali
id: 7e146587-8972-11ed-ae7b-d7a32ea86a81
last_name: Vistunou
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Mojtaba
full_name: Tavakoli, Mojtaba
id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87
last_name: Tavakoli
orcid: 0000-0002-7667-6854
- first_name: Nathalie
full_name: Agudelo Duenas, Nathalie
id: 40E7F008-F248-11E8-B48F-1D18A9856A87
last_name: Agudelo Duenas
- first_name: Jakob
full_name: Vorlaufer, Jakob
id: 937696FA-C996-11E9-8C7C-CF13E6697425
last_name: Vorlaufer
orcid: 0009-0000-7590-3501
- first_name: Wiebke
full_name: Jahr, Wiebke
id: 425C1CE8-F248-11E8-B48F-1D18A9856A87
last_name: Jahr
orcid: 0000-0003-0201-2315
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion
microscopy in plant roots. The Plant Cell. 2025;37(4). doi:10.1093/plcell/koaf006
apa: Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou,
V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy
in plant roots. The Plant Cell. Oxford University Press. https://doi.org/10.1093/plcell/koaf006
chicago: Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella,
Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution
Expansion Microscopy in Plant Roots.” The Plant Cell. Oxford University
Press, 2025. https://doi.org/10.1093/plcell/koaf006.
ieee: M. C. Gallei et al., “Super-resolution expansion microscopy in plant
roots,” The Plant Cell, vol. 37, no. 4. Oxford University Press, 2025.
ista: Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM,
Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková
E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots.
The Plant Cell. 37(4), koaf006.
mla: Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant
Roots.” The Plant Cell, vol. 37, no. 4, koaf006, Oxford University Press,
2025, doi:10.1093/plcell/koaf006.
short: M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou,
C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch,
A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).
corr_author: '1'
date_created: 2025-02-05T06:52:06Z
date_published: 2025-04-01T00:00:00Z
date_updated: 2026-10-01T11:19:12Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JoDa
- _id: JiFr
doi: 10.1093/plcell/koaf006
ec_funded: 1
external_id:
isi:
- '001462763100001'
pmid:
- '39792900'
file:
- access_level: open_access
checksum: 9d3f8218ff37a29f29c48a7bbe831bd3
content_type: application/pdf
creator: dernst
date_created: 2025-07-31T07:03:43Z
date_updated: 2025-07-31T07:03:43Z
file_id: '20092'
file_name: 2025_PlantCell_Gallei.pdf
file_size: 53904111
relation: main_file
success: 1
file_date_updated: 2025-07-31T07:03:43Z
fulldoi: https://doi.org/10.1093/plcell/koaf006
has_accepted_license: '1'
intvolume: ' 37'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: 269B5B22-B435-11E9-9278-68D0E5697425
grant_number: ALTF 679-2018
name: UltraX - achieving sub-nanometer resolution in light microscopy using iterative
X10 microscopy in combination with nanobodies and STED
- _id: 6285a163-2b32-11ec-9570-8e204ca2dba5
grant_number: '26137'
name: Studying Organelle Structure and Function at Nanoscale Resolution with Expansion
Microscopy
- _id: 26AA4EF2-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
record:
- id: '18837'
relation: research_data
status: public
- id: '18689'
relation: earlier_version
status: public
scopus_import: '1'
status: public
title: Super-resolution expansion microscopy in plant roots
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 37
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20187'
abstract:
- lang: eng
text: Very long-chain fatty acids (VLCFAs), being constituents of different types
of lipids, are critical factors in plant development, presumably due to their
impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic
reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase
1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems
(esm) mutant, a viable kcr1 allele presumably affecting surface properties of
the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that
impacts VLCFAs. The kcr1-2 plants show severe defects during different stages
of development, which all correlate with defects in polar localization and subcellular
trafficking of PIN auxin transporters and resulting asymmetric auxin distribution.
Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests
that KCR1 plays a role in delineating boundaries around meristematic and specialized
differentiating tissues, including root and shoot meristems, initiating lateral
roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced
VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful
tool to study VLCFA roles in plant development and highlights VLCFAs as critical
developmental factors at the interface of cell polarity and tissue development.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: "We gratefully acknowledge the Imaging and Optics, Electron Microscopy
(especially Vanessa Zheden for technical assistance) and Life Science (in particular
Dorota Jaworska) facilities at ISTA for their continuous support. Authors would
like to thank Michelle Gallei for advice during the generation of the transgenic
lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help
and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and
root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski
for advice on abiotic stress experiments. We would like to thank Dolf Weijers for
the SOSEKI (SOK) marker line seeds. This work has benefited from the support of
IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian
Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province
(20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant
Sciences-SPS (ANR-17-EUR-0007)."
article_number: e70396
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: David
full_name: Babic, David
id: db566d23-f6e0-11ea-865d-e6f270e968e7
last_name: Babic
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Lukas
full_name: Fiedler, Lukas
id: 7c417475-8972-11ed-ae7b-8b674ca26986
last_name: Fiedler
- first_name: Linlin
full_name: Qi, Linlin
id: 44B04502-A9ED-11E9-B6FC-583AE6697425
last_name: Qi
orcid: 0000-0001-5187-8401
- first_name: Frédérique
full_name: Tellier, Frédérique
last_name: Tellier
- first_name: Adrijana
full_name: Smoljan, Adrijana
id: cced8a85-223e-11ed-af04-b0596c55053b
last_name: Smoljan
- first_name: Hana
full_name: Rakusova, Hana
id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA
last_name: Rakusova
- first_name: Petr
full_name: Valošek, Petr
id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
last_name: Valošek
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jean Denis
full_name: Faure, Jean Denis
last_name: Faure
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty
acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
development. Plant Journal. 2025;123(3). doi:10.1111/tpj.70396
apa: Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml,
J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis
auxin-mediated embryonic and post-embryonic development. Plant Journal.
Wiley. https://doi.org/10.1111/tpj.70396
chicago: Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier,
Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty
Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic
Development.” Plant Journal. Wiley, 2025. https://doi.org/10.1111/tpj.70396.
ieee: D. Babic et al., “Biosynthesis of very long-chain fatty acids is required
for Arabidopsis auxin-mediated embryonic and post-embryonic development,” Plant
Journal, vol. 123, no. 3. Wiley, 2025.
ista: Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek
P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain
fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic
development. Plant Journal. 123(3), e70396.
mla: Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required
for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” Plant
Journal, vol. 123, no. 3, e70396, Wiley, 2025, doi:10.1111/tpj.70396.
short: D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova,
P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).
corr_author: '1'
date_created: 2025-08-17T22:01:36Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-10-16T07:59:32Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
- _id: GradSch
doi: 10.1111/tpj.70396
external_id:
isi:
- '001547884300001'
pmid:
- '40782342'
file:
- access_level: open_access
checksum: 1cdc3341d2d23101abca72521f1f23cb
content_type: application/pdf
creator: dernst
date_created: 2025-09-01T14:09:31Z
date_updated: 2025-09-01T14:09:31Z
file_id: '20264'
file_name: 2025_PlantJournal_Babic.pdf
file_size: 5791111
relation: main_file
success: 1
file_date_updated: 2025-09-01T14:09:31Z
fulldoi: https://doi.org/10.1111/tpj.70396
has_accepted_license: '1'
intvolume: ' 123'
isi: 1
issue: '3'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
publication: Plant Journal
publication_identifier:
eissn:
- 1365-313X
issn:
- 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
related_material:
record:
- id: '20362'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated
embryonic and post-embryonic development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 123
year: '2025'
...
---
_id: '14842'
abstract:
- lang: eng
text: Eva Benkova received a PhD in Biophysics at the Institute of Biophysics of
the Czech Academy of Sciences in 1998. After working as a postdoc at the Max Planck
Institute in Cologne and the Center for Plant Molecular Biology (ZMBP) in Tübingen,
she became a group leader at the Plant Systems Biology Department of the Vlaams
Instituut voor Biotechnologie (VIB) in Gent. In 2012, she transitioned to an Assistant
Professor position at the Institute of Science and Technology Austria (ISTA) where
she was later promoted to Professor. Since 2021, she has served as the Dean of
the ISTA Graduate School. As a plant developmental biologist, she focuses on unraveling
the molecular mechanisms and principles that underlie hormonal interactions in
plants. In her current work, she explores the intricate connections between hormones
and regulatory pathways that mediate the perception of environmental stimuli,
including abiotic stress and nitrate availability.
article_processing_charge: No
author:
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Benková E. Eva Benkova. Vol 34. Elsevier; 2024:R3-R5. doi:10.1016/j.cub.2023.11.039
apa: Benková, E. (2024). Eva Benkova. Current Biology (Vol. 34, pp.
R3–R5). Elsevier. https://doi.org/10.1016/j.cub.2023.11.039
chicago: Benková, Eva. Eva Benkova. Current Biology. Vol. 34. Elsevier,
2024. https://doi.org/10.1016/j.cub.2023.11.039.
ieee: E. Benková, Eva Benkova, vol. 34, no. 1. Elsevier, 2024, pp. R3–R5.
ista: Benková E. 2024. Eva Benkova, Elsevier,p.
mla: Benková, Eva. “Eva Benkova.” Current Biology, vol. 34, no. 1, Elsevier,
2024, pp. R3–5, doi:10.1016/j.cub.2023.11.039.
short: E. Benková, Eva Benkova, Elsevier, 2024.
corr_author: '1'
das_tickbox: '0'
date_created: 2024-01-21T23:00:56Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2026-10-01T11:17:58Z
day: '08'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.1016/j.cub.2023.11.039
external_id:
pmid:
- '38194926'
fulldoi: https://doi.org/10.1016/j.cub.2023.11.039
intvolume: ' 34'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/j.cub.2023.11.039
month: '01'
oa: 1
oa_version: Published Version
page: R3-R5
pmid: 1
publication: Current Biology
publication_identifier:
eissn:
- 1879-0445
publication_status: published
publisher: Elsevier
quality_controlled: '1'
researchdata_availability: not applicable
status: public
supplementarymaterial: no
title: Eva Benkova
type: other_academic_publication
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 34
year: '2024'
...
---
OA_place: repository
_id: '18689'
abstract:
- lang: eng
text: Multiplexed fluorescence microscopy imaging is widely used in biomedical applications.
However, simultaneous imaging of multiple fluorophores can result in spectral
leaks and overlapping, which greatly degrades image quality and subsequent analysis.
Existing popular spectral unmixing methods are mainly based on computational intensive
linear models and the performance is heavily dependent on the reference spectra,
which may greatly preclude its further applications. In this paper, we propose
a deep learning-based blindly spectral unmixing method, termed AutoUnmix, to imitate
the physical spectral mixing process. A tranfer learning framework is further
devised to allow our AutoUnmix adapting to a variety of imaging systems without
retraining the network. Our proposed method has demonstrated real-time unmixing
capabilities, surpassing existing methods by up to 100-fold in terms of unmixing
speed. We further validate the reconstruction performance on both synthetic datasets
and biological samples. The unmixing results of AutoUnmix achieve a highest SSIM
of 0.99 in both three- and four-color imaging, with nearly up to 20% higher than
other popular unmixing methods. Due to the desirable property of data independency
and superior blind unmixing performance, we believe AutoUnmix is a powerful tool
to study the interaction process of different organelles labeled by multiple fluorophores.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: M-Shop
- _id: E-Lib
acknowledgement: "We gratefully acknowledge support by the Scientific Service Units
at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical
workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\n\r\nThis
project has received funding from the Austrian Science Fund (FWF): I 3630-B25 (J.G.D)
and the European Research Council (ERC) under the European Union’s Horizon 2020
research and innovation programme (grant agreement No 742985, J.F.). It has also
received funding from the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie Grant Agreement No. 665385. S.T. has
received funding as an ISTplus Fellow from the European Union’s Horizon 2020 Research
and Innovation Programme under Marie Skłodowska-Curie grant agreement no. 754411
and from an EMBO Long-Term Fellowship (grant number ALTF 679-2018). It has further
received funding from the Austrian Science Fund (FWF) grant DK W1232 (M.T, N.A-D.,
J.G.D). W.J. received funding via a Human Frontier Science Program postdoctoral
fellowship LT000557/2018.\r\n\r\nThe funders had no role in study design, data collection
and analysis, decision to publish or preparation of the manuscript."
article_processing_charge: No
author:
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Sven M
full_name: Truckenbrodt, Sven M
id: 45812BD4-F248-11E8-B48F-1D18A9856A87
last_name: Truckenbrodt
- first_name: Caroline
full_name: Kreuzinger, Caroline
id: 382077BA-F248-11E8-B48F-1D18A9856A87
last_name: Kreuzinger
- first_name: Syamala
full_name: Inumella, Syamala
id: F8660870-D756-11E9-98C5-34DFE5697425
last_name: Inumella
orcid: 0009-0002-5890-120X
- first_name: Vitali
full_name: Vistunou, Vitali
id: 7e146587-8972-11ed-ae7b-d7a32ea86a81
last_name: Vistunou
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Mojtaba
full_name: Tavakoli, Mojtaba
id: 3A0A06F4-F248-11E8-B48F-1D18A9856A87
last_name: Tavakoli
orcid: 0000-0002-7667-6854
- first_name: Nathalie
full_name: Agudelo Duenas, Nathalie
id: 40E7F008-F248-11E8-B48F-1D18A9856A87
last_name: Agudelo Duenas
- first_name: Jakob
full_name: Vorlaufer, Jakob
id: 937696FA-C996-11E9-8C7C-CF13E6697425
last_name: Vorlaufer
orcid: 0009-0000-7590-3501
- first_name: Wiebke
full_name: Jahr, Wiebke
id: 425C1CE8-F248-11E8-B48F-1D18A9856A87
last_name: Jahr
orcid: 0000-0003-0201-2315
- first_name: Marek
full_name: Randuch, Marek
id: 6ac4636d-15b2-11ec-abd3-fb8df79972ae
last_name: Randuch
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion
microscopy in plant roots. bioRxiv. doi:10.1101/2024.02.21.581330
apa: Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou,
V., Sommer, C. M., … Danzl, J. G. (n.d.). Super-resolution expansion microscopy
in plant roots. bioRxiv. https://doi.org/10.1101/2024.02.21.581330
chicago: Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella,
Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution
Expansion Microscopy in Plant Roots.” BioRxiv, n.d. https://doi.org/10.1101/2024.02.21.581330.
ieee: M. C. Gallei et al., “Super-resolution expansion microscopy in plant
roots,” bioRxiv. .
ista: Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM,
Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková
E, Friml J, Danzl JG. Super-resolution expansion microscopy in plant roots. bioRxiv,
10.1101/2024.02.21.581330.
mla: Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant
Roots.” BioRxiv, doi:10.1101/2024.02.21.581330.
short: M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou,
C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch,
A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, BioRxiv (n.d.).
corr_author: '1'
das_tickbox: '0'
date_created: 2024-12-19T12:28:00Z
date_published: 2024-02-21T00:00:00Z
date_updated: 2026-10-02T11:27:43Z
day: '21'
department:
- _id: EvBe
- _id: JoDa
- _id: JiFr
doi: 10.1101/2024.02.21.581330
ec_funded: 1
fulldoi: https://doi.org/10.1101/2024.02.21.581330
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2024.02.21.581330
month: '02'
oa: 1
oa_version: Preprint
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: 26AA4EF2-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
- _id: 269B5B22-B435-11E9-9278-68D0E5697425
grant_number: ALTF 679-2018
name: UltraX - achieving sub-nanometer resolution in light microscopy using iterative
X10 microscopy in combination with nanobodies and STED
publication: bioRxiv
publication_status: draft
related_material:
record:
- id: '19003'
relation: later_version
status: public
- id: '18681'
relation: dissertation_contains
status: public
researchdata_availability: no
status: public
supplementarymaterial: no
title: Super-resolution expansion microscopy in plant roots
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: preprint
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2024'
...
---
_id: '15301'
abstract:
- lang: eng
text: Plant morphogenesis relies exclusively on oriented cell expansion and division.
Nonetheless, the mechanism(s) determining division plane orientation remain elusive.
Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis
thaliana and uncovered how mechanical forces stabilize and reorient the microtubule
cytoskeleton for the orientation of cell division. We identified that root tissue
functions as an interconnected cell matrix, with a radial gradient of tissue extendibility
causing predictable tissue deformation after wounding. This deformation causes
instant redirection of expansion in the surrounding cells and reorientation of
microtubule arrays, ultimately predicting cell division orientation. Microtubules
are destabilized under low tension, whereas stretching of cells, either through
wounding or external aspiration, immediately induces their polymerization. The
higher microtubule abundance in the stretched cell parts leads to the reorientation
of microtubule arrays and, ultimately, informs cell division planes. This provides
a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical
forces for tissue reconstruction and plant architecture.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder
for sharing published material. We thank the Imaging & Optics and Life Science Facilities
at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility
at DBMV UNIL for providing invaluable assistance. The research leading to these
results has received funding from the European Research Council under the European
Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985,
from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: N
full_name: Trozzi, N
last_name: Trozzi
- first_name: Leonhard
full_name: Spona, Leonhard
id: b52391fb-f636-11ee-939c-8a8c47552e8a
last_name: Spona
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
orcid: 0000-0001-6111-9353
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Silvia
full_name: Caballero Mancebo, Silvia
id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
last_name: Caballero Mancebo
orcid: 0000-0002-5223-3346
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Y
full_name: Dagdas, Y
last_name: Dagdas
- first_name: M
full_name: Majda, M
last_name: Majda
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant
tissue matrix orient cell divisions via microtubule stabilization. Developmental
Cell. 2024;59(10):1333-1344.e4. doi:10.1016/j.devcel.2024.03.009
apa: Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S.,
Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient
cell divisions via microtubule stabilization. Developmental Cell. Elsevier.
https://doi.org/10.1016/j.devcel.2024.03.009
chicago: Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko
Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in
Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” Developmental
Cell. Elsevier, 2024. https://doi.org/10.1016/j.devcel.2024.03.009.
ieee: L. Hörmayer et al., “Mechanical forces in plant tissue matrix orient
cell divisions via microtubule stabilization,” Developmental Cell, vol.
59, no. 10. Elsevier, p. 1333–1344.e4, 2024.
ista: Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P,
Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024.
Mechanical forces in plant tissue matrix orient cell divisions via microtubule
stabilization. Developmental Cell. 59(10), 1333–1344.e4.
mla: Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell
Divisions via Microtubule Stabilization.” Developmental Cell, vol. 59,
no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:10.1016/j.devcel.2024.03.009.
short: L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá,
S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J.
Friml, Developmental Cell 59 (2024) 1333–1344.e4.
corr_author: '1'
das_tickbox: '1'
dataavailabilitystatement: "All data reported in this paper will be shared by the
lead contact upon request.\r\n\r\nThis paper does not report original code.\r\n\r\nAny
additional information required to reanalyze the data reported in this paper is
available from the lead contact upon request."
date_created: 2024-04-08T12:07:57Z
date_published: 2024-05-20T00:00:00Z
date_updated: 2026-10-15T08:32:32Z
day: '20'
ddc:
- '570'
department:
- _id: JiFr
- _id: EvBe
- _id: CaHe
doi: 10.1016/j.devcel.2024.03.009
ec_funded: 1
external_id:
isi:
- '001301584600001'
pmid:
- '38579717'
file:
- access_level: open_access
checksum: 22b374fb50a40d380b7686c84258d271
content_type: application/pdf
creator: dernst
date_created: 2024-08-20T11:22:16Z
date_updated: 2024-08-20T11:22:16Z
file_id: '17452'
file_name: 2024_DevelopmentalCell_Hoermayer.pdf
file_size: 5195262
relation: main_file
success: 1
file_date_updated: 2024-08-20T11:22:16Z
fulldoi: https://doi.org/10.1016/j.devcel.2024.03.009
has_accepted_license: '1'
intvolume: ' 59'
isi: 1
issue: '10'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1333-1344.e4
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Developmental Cell
publication_identifier:
eissn:
- 1878-1551
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/how-plants-heal-wounds/
researchdata_availability: upon request
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Mechanical forces in plant tissue matrix orient cell divisions via microtubule
stabilization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 59
year: '2024'
...
---
OA_place: publisher
OA_type: hybrid
_id: '18596'
abstract:
- lang: eng
text: Hormone perception and signaling pathways have a fundamental regulatory function
in the physiological processes of plants. Cytokinins, a class of plant hormones,
regulate cell division and meristem maintenance. The cytokinin signaling pathway
is well established in the model plant Arabidopsis thaliana. Several negative
feedback mechanisms, tightly controlling cytokinin signaling output, have been
described previously. In this study, we identified a new feedback mechanism executed
through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing
variant named CRE1int7 results from seventh intron retention, introducing a premature
termination codon in the transcript. We showed that CRE1int7 is translated in
planta into a truncated receptor lacking the C-terminal receiver domain essential
for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream
cascade. We present a novel negative feedback mechanism of the cytokinin signaling
pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin
receptors via dimerization and compete with them for ligand binding. Ensuring
proper plant growth and development requires precise control of the cytokinin
signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism
for fine-tuning the cytokinin signaling pathway in Arabidopsis.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We dedicate this paper to the deceased Petr Galuszka for his inspiration
and support of our project. We thank Prof. Peter Hedden for constructive criticism
of the manuscript and English editing. No conflict of interest is declared.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Michaela
full_name: Králová, Michaela
last_name: Králová
- first_name: Ivona
full_name: Kubalová, Ivona
last_name: Kubalová
- first_name: Jakub
full_name: Hajný, Jakub
last_name: Hajný
- first_name: Karolina
full_name: Kubiasova, Karolina
id: 946011F4-3E71-11EA-860B-C7A73DDC885E
last_name: Kubiasova
orcid: 0000-0001-5630-9419
- first_name: Karolína
full_name: Vagaská, Karolína
last_name: Vagaská
- first_name: Zengxiang
full_name: Ge, Zengxiang
id: f43371a3-09ff-11eb-8013-bd0c6a2f6de8
last_name: Ge
orcid: 0000-0001-9381-3577
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Hana
full_name: Semerádová, Hana
id: 42FE702E-F248-11E8-B48F-1D18A9856A87
last_name: Semerádová
- first_name: Anna
full_name: Kuchařová, Anna
last_name: Kuchařová
- first_name: Martin
full_name: Hönig, Martin
last_name: Hönig
- first_name: Aline
full_name: Monzer, Aline
id: 2DB5D88C-D7B3-11E9-B8FD-7907E6697425
last_name: Monzer
- first_name: Martin
full_name: Kovačik, Martin
last_name: Kovačik
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Yoshihisa
full_name: Ikeda, Yoshihisa
last_name: Ikeda
- first_name: David
full_name: Zalabák, David
last_name: Zalabák
citation:
ama: Králová M, Kubalová I, Hajný J, et al. A decoy receptor derived from alternative
splicing fine-tunes cytokinin signaling in Arabidopsis. Molecular Plant.
2024;17(12):1850-1865. doi:10.1016/j.molp.2024.11.001
apa: Králová, M., Kubalová, I., Hajný, J., Kubiasova, K., Vagaská, K., Ge, Z., …
Zalabák, D. (2024). A decoy receptor derived from alternative splicing fine-tunes
cytokinin signaling in Arabidopsis. Molecular Plant. Elsevier. https://doi.org/10.1016/j.molp.2024.11.001
chicago: Králová, Michaela, Ivona Kubalová, Jakub Hajný, Karolina Kubiasova, Karolína
Vagaská, Zengxiang Ge, Michelle C Gallei, et al. “A Decoy Receptor Derived from
Alternative Splicing Fine-Tunes Cytokinin Signaling in Arabidopsis.” Molecular
Plant. Elsevier, 2024. https://doi.org/10.1016/j.molp.2024.11.001.
ieee: M. Králová et al., “A decoy receptor derived from alternative splicing
fine-tunes cytokinin signaling in Arabidopsis,” Molecular Plant, vol. 17,
no. 12. Elsevier, pp. 1850–1865, 2024.
ista: Králová M, Kubalová I, Hajný J, Kubiasova K, Vagaská K, Ge Z, Gallei MC, Semerádová
H, Kuchařová A, Hönig M, Monzer A, Kovačik M, Friml J, Novák O, Benková E, Ikeda
Y, Zalabák D. 2024. A decoy receptor derived from alternative splicing fine-tunes
cytokinin signaling in Arabidopsis. Molecular Plant. 17(12), 1850–1865.
mla: Králová, Michaela, et al. “A Decoy Receptor Derived from Alternative Splicing
Fine-Tunes Cytokinin Signaling in Arabidopsis.” Molecular Plant, vol. 17,
no. 12, Elsevier, 2024, pp. 1850–65, doi:10.1016/j.molp.2024.11.001.
short: M. Králová, I. Kubalová, J. Hajný, K. Kubiasova, K. Vagaská, Z. Ge, M.C.
Gallei, H. Semerádová, A. Kuchařová, M. Hönig, A. Monzer, M. Kovačik, J. Friml,
O. Novák, E. Benková, Y. Ikeda, D. Zalabák, Molecular Plant 17 (2024) 1850–1865.
das_tickbox: '0'
date_created: 2024-11-28T11:13:35Z
date_published: 2024-12-02T00:00:00Z
date_updated: 2026-10-15T08:30:20Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.molp.2024.11.001
external_id:
isi:
- '001373778300001'
pmid:
- '39501563'
file:
- access_level: open_access
checksum: a11feea4b1677df76b632eca04bfc1dd
content_type: application/pdf
creator: dernst
date_created: 2024-12-03T11:08:09Z
date_updated: 2024-12-03T11:08:09Z
file_id: '18612'
file_name: 2024_MolecularPlant_Kralova.pdf
file_size: 3308945
relation: main_file
success: 1
file_date_updated: 2024-12-03T11:08:09Z
fulldoi: https://doi.org/10.1016/j.molp.2024.11.001
has_accepted_license: '1'
intvolume: ' 17'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 1850-1865
pmid: 1
publication: Molecular Plant
publication_identifier:
issn:
- 1674-2052
publication_status: published
publisher: Elsevier
quality_controlled: '1'
researchdata_availability: no
scopus_import: '1'
status: public
supplementarymaterial: yes
title: A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling
in Arabidopsis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 17
year: '2024'
...
---
_id: '18063'
abstract:
- lang: eng
text: The developmental plasticity of the root system plays an essential role in
the adaptation of plants to the environment. Among many other signals, auxin and
its directional, intercellular transport are critical in regulating root growth
and development. In particular, the PIN-FORMED2 (PIN2) auxin exporter acts as
a key regulator of root gravitropic growth. Multiple regulators have been reported
to be involved in PIN2-mediated root growth; however, our information remains
incomplete. Here, we identified ROWY Bro1-domain proteins as important regulators
of PIN2 sorting control. Genetic analysis revealed that Arabidopsis rowy1 single
mutants and higher-order rowy1 rowy2 rowy3 triple mutants presented a wavy root
growth phenotype. Cell biological experiments revealed that ROWY1 and PIN2 colocalized
to the apical side of the plasma membrane in the root epidermis and that ROWYs
are required for correct PM targeting of PIN2. In addition, ROWYs also affected
PIN3 protein abundance in the stele, suggesting the potential involvement of additional
PIN transporters as well as other proteins. A global transcriptome analysis revealed
that ROWY genes are involved in the Fe2+ availability perception pathway. This
work establishes ROWYs as important novel regulators of root gravitropic growth
by connecting micronutrient availability to the proper subcellular targeting of
PIN auxin transporters.
acknowledgement: We thank Drs. Erika Isono (University of Constance), Grégory Vert
(University of Toulouse), and Liwen Jiang (The Chinese University of Hong Kong)
for kindly sharing published Arabidopsis lines; Dr. Yuzhou Zhang (ISTA) for help
with molecular cloning, and Drs. Melinda Abas (BOKU), Eugenia Russinova (Ghent University),
and Zhaojun Ding (Shandong University) for valuable discussions. This work was supported
by grants to S.T. from the National Natural Science Foundation of China (32321001),
the USTC Research Funds of the Double First-Class Initiative (YD9100002016), the
Research Funds from the Center for Advanced Interdisciplinary Science and Biomedicine
of IHM, the Division of Life Sciences and Medicine, the University of Science and
Technology of China (QYPY20220012), the Fundamental Research Funds for the Central
Universities (WK9100000021), and start-up funding from the University of Science
and Technology of China and the Chinese Academy of Sciences (GG9100007007, KY9100000026,
KY9100000051, and KJ2070000079). J.S. was supported by the National Natural Science
Foundation of China (31970181 and 32170342). J.F. was supported by Austrian Science
Fund (FWF; projects I6123 and P37051-B).
article_number: '1085'
article_processing_charge: Yes
article_type: original
author:
- first_name: Yakun
full_name: Peng, Yakun
last_name: Peng
- first_name: Kangkang
full_name: Ji, Kangkang
last_name: Ji
- first_name: Yanbo
full_name: Mao, Yanbo
last_name: Mao
- first_name: Yiqun
full_name: Wang, Yiqun
id: 82F537F2-B517-11E9-84D7-6433E6697425
last_name: Wang
- first_name: Barbara
full_name: Korbei, Barbara
last_name: Korbei
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Jinbo
full_name: Shen, Jinbo
last_name: Shen
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
citation:
ama: Peng Y, Ji K, Mao Y, et al. Polarly localized Bro1 domain proteins regulate
PIN-FORMED abundance and root gravitropic growth in Arabidopsis. Communications
Biology. 2024;7. doi:10.1038/s42003-024-06747-9
apa: Peng, Y., Ji, K., Mao, Y., Wang, Y., Korbei, B., Luschnig, C., … Tan, S. (2024).
Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root
gravitropic growth in Arabidopsis. Communications Biology. Springer Nature.
https://doi.org/10.1038/s42003-024-06747-9
chicago: Peng, Yakun, Kangkang Ji, Yanbo Mao, Yiqun Wang, Barbara Korbei, Christian
Luschnig, Jinbo Shen, Eva Benková, Jiří Friml, and Shutang Tan. “Polarly Localized
Bro1 Domain Proteins Regulate PIN-FORMED Abundance and Root Gravitropic Growth
in Arabidopsis.” Communications Biology. Springer Nature, 2024. https://doi.org/10.1038/s42003-024-06747-9.
ieee: Y. Peng et al., “Polarly localized Bro1 domain proteins regulate PIN-FORMED
abundance and root gravitropic growth in Arabidopsis,” Communications Biology,
vol. 7. Springer Nature, 2024.
ista: Peng Y, Ji K, Mao Y, Wang Y, Korbei B, Luschnig C, Shen J, Benková E, Friml
J, Tan S. 2024. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance
and root gravitropic growth in Arabidopsis. Communications Biology. 7, 1085.
mla: Peng, Yakun, et al. “Polarly Localized Bro1 Domain Proteins Regulate PIN-FORMED
Abundance and Root Gravitropic Growth in Arabidopsis.” Communications Biology,
vol. 7, 1085, Springer Nature, 2024, doi:10.1038/s42003-024-06747-9.
short: Y. Peng, K. Ji, Y. Mao, Y. Wang, B. Korbei, C. Luschnig, J. Shen, E. Benková,
J. Friml, S. Tan, Communications Biology 7 (2024).
das_tickbox: '1'
dataavailabilitystatement: 'The RNA-seq data for this study are available online at
the NCBI Sequence Read Archive (accession number: PRJNA1116831). Uncropped images
of the DNA gels are provided in the Supplementary Figs. 21 to 22. The source data
used to generate the plots are provided in the Supplementary Data 2.'
date_created: 2024-09-15T22:01:38Z
date_published: 2024-09-04T00:00:00Z
date_updated: 2026-10-15T22:31:28Z
day: '04'
ddc:
- '570'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/s42003-024-06747-9
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creator: dernst
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file_name: 2024_CommBiology_Peng.pdf
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file_date_updated: 2024-09-17T09:44:29Z
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isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: bd76d395-d553-11ed-ba76-f678c14f9033
grant_number: I06123
name: Peptide receptors for auxin canalization in Arabidopsis
- _id: 7bcece63-9f16-11ee-852c-ae94e099eeb6
grant_number: P37051
name: Guanylate cyclase activity of TIR1/AFBs auxin receptors
publication: Communications Biology
publication_identifier:
eissn:
- 2399-3642
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '20117'
relation: dissertation_contains
status: public
researchdata_availability: yes (supplementary data)
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root
gravitropic growth in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 7
year: '2024'
...
---
_id: '13214'
abstract:
- lang: eng
text: Nitrogen is an important macronutrient required for plant growth and development,
thus directly impacting agricultural productivity. In recent years, numerous studies
have shown that nitrogen-driven growth depends on pathways that control nitrate/nitrogen
homeostasis and hormonal networks that act both locally and systemically to coordinate
growth and development of plant organs. In this review, we will focus on recent
advances in understanding the role of the plant hormones auxin and cytokinin and
their crosstalk in nitrate-regulated growth and discuss the significance of novel
findings and possible missing links.
acknowledgement: 'This work was supported by the Austrian Academy of Sciences ÖAW:
Doc fellowship (26130) to Stefan Riegler.'
article_number: '1613'
article_processing_charge: Yes
article_type: review
author:
- first_name: R
full_name: Abualia, R
last_name: Abualia
- first_name: Stefan
full_name: Riegler, Stefan
id: FF6018E0-D806-11E9-8E43-0B14E6697425
last_name: Riegler
orcid: 0000-0003-3413-1343
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Abualia R, Riegler S, Benková E. Nitrate, auxin and cytokinin - a trio to tango.
Cells. 2023;12(12). doi:10.3390/cells12121613
apa: Abualia, R., Riegler, S., & Benková, E. (2023). Nitrate, auxin and cytokinin
- a trio to tango. Cells. MDPI. https://doi.org/10.3390/cells12121613
chicago: Abualia, R, Stefan Riegler, and Eva Benková. “Nitrate, Auxin and Cytokinin
- a Trio to Tango.” Cells. MDPI, 2023. https://doi.org/10.3390/cells12121613.
ieee: R. Abualia, S. Riegler, and E. Benková, “Nitrate, auxin and cytokinin - a
trio to tango,” Cells, vol. 12, no. 12. MDPI, 2023.
ista: Abualia R, Riegler S, Benková E. 2023. Nitrate, auxin and cytokinin - a trio
to tango. Cells. 12(12), 1613.
mla: Abualia, R., et al. “Nitrate, Auxin and Cytokinin - a Trio to Tango.” Cells,
vol. 12, no. 12, 1613, MDPI, 2023, doi:10.3390/cells12121613.
short: R. Abualia, S. Riegler, E. Benková, Cells 12 (2023).
corr_author: '1'
date_created: 2023-07-12T07:41:25Z
date_published: 2023-06-13T00:00:00Z
date_updated: 2025-04-15T06:27:18Z
day: '13'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.3390/cells12121613
external_id:
isi:
- '001017033600001'
pmid:
- '37371083'
file:
- access_level: open_access
checksum: 6dc9df5f4f59fc27c509c275060354a5
content_type: application/pdf
creator: alisjak
date_created: 2023-07-12T10:01:54Z
date_updated: 2023-07-12T10:01:54Z
file_id: '13218'
file_name: 2023_cells_Abualia.pdf
file_size: 1066802
relation: main_file
success: 1
file_date_updated: 2023-07-12T10:01:54Z
fulldoi: https://doi.org/10.3390/cells12121613
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '12'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 62883ed7-2b32-11ec-9570-93580204e56b
grant_number: '26130'
name: Functional asymmetry of medial habenula outputs in mice
publication: Cells
publication_identifier:
issn:
- 2073-4409
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Nitrate, auxin and cytokinin - a trio to tango
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2023'
...
---
_id: '11734'
abstract:
- lang: eng
text: Mineral nutrition is one of the key environmental factors determining plant
development and growth. Nitrate is the major form of macronutrient nitrogen that
plants take up from the soil. Fluctuating availability or deficiency of this element
severely limits plant growth and negatively affects crop production in the agricultural
system. To cope with the heterogeneity of nitrate distribution in soil, plants
evolved a complex regulatory mechanism that allows rapid adjustment of physiological
and developmental processes to the status of this nutrient. The root, as a major
exploitation organ that controls the uptake of nitrate to the plant body, acts
as a regulatory hub that, according to nitrate availability, coordinates the growth
and development of other plant organs. Here, we identified a regulatory framework,
where cytokinin response factors (CRFs) play a central role as a molecular readout
of the nitrate status in roots to guide shoot adaptive developmental response.
We show that nitrate-driven activation of NLP7, a master regulator of nitrate
response in plants, fine tunes biosynthesis of cytokinin in roots and its translocation
to shoots where it enhances expression of CRFs. CRFs, through direct transcriptional
regulation of PIN auxin transporters, promote the flow of auxin and thereby stimulate
the development of shoot organs.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We acknowledge Hana Semeradova, Juan Carlos Montesinos, Nicola Cavallari,
Marc¸al Gallem\x03ı, Kaori Tabata, Andrej Hurn\x03y, and Sascha Waidmann for sharing
materials; and Marina Borges Osorio for critical reading of the manuscript. Work
in the E. Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S)
to K.O., R.A., and E. Benkova. We acknowledge the Bioimaging Facility and Life Science
Facilities of the Institute of Science\r\nand Technology Austria. We give sincere
thanks to Hana Martınkova and Petra Amakorova for their help with cytokinin analyses.
This work was funded by the Czech Science Foundation (Project No. 19-00973S)."
article_number: e2122460119
article_processing_charge: No
article_type: original
author:
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Eleonore
full_name: Bouguyon, Eleonore
last_name: Bouguyon
- first_name: Kevin
full_name: Domanegg, Kevin
id: a24c7829-16e8-11ed-8527-c4d36ffb7539
last_name: Domanegg
orcid: 0000-0002-1215-4264
- first_name: Anne
full_name: Krapp, Anne
last_name: Krapp
- first_name: Philip
full_name: Nacry, Philip
last_name: Nacry
- first_name: Alain
full_name: Gojon, Alain
last_name: Gojon
- first_name: Benoit
full_name: Lacombe, Benoit
last_name: Lacombe
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Abualia R, Ötvös K, Novák O, et al. Molecular framework integrating nitrate
sensing in root and auxin-guided shoot adaptive responses. Proceedings of the
National Academy of Sciences of the United States of America. 2022;119(31).
doi:10.1073/pnas.2122460119
apa: Abualia, R., Ötvös, K., Novák, O., Bouguyon, E., Domanegg, K., Krapp, A., …
Benková, E. (2022). Molecular framework integrating nitrate sensing in root and
auxin-guided shoot adaptive responses. Proceedings of the National Academy
of Sciences of the United States of America. National Academy of Sciences.
https://doi.org/10.1073/pnas.2122460119
chicago: Abualia, Rashed, Krisztina Ötvös, Ondřej Novák, Eleonore Bouguyon, Kevin
Domanegg, Anne Krapp, Philip Nacry, Alain Gojon, Benoit Lacombe, and Eva Benková.
“Molecular Framework Integrating Nitrate Sensing in Root and Auxin-Guided Shoot
Adaptive Responses.” Proceedings of the National Academy of Sciences of the
United States of America. National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2122460119.
ieee: R. Abualia et al., “Molecular framework integrating nitrate sensing
in root and auxin-guided shoot adaptive responses,” Proceedings of the National
Academy of Sciences of the United States of America, vol. 119, no. 31. National
Academy of Sciences, 2022.
ista: Abualia R, Ötvös K, Novák O, Bouguyon E, Domanegg K, Krapp A, Nacry P, Gojon
A, Lacombe B, Benková E. 2022. Molecular framework integrating nitrate sensing
in root and auxin-guided shoot adaptive responses. Proceedings of the National
Academy of Sciences of the United States of America. 119(31), e2122460119.
mla: Abualia, Rashed, et al. “Molecular Framework Integrating Nitrate Sensing in
Root and Auxin-Guided Shoot Adaptive Responses.” Proceedings of the National
Academy of Sciences of the United States of America, vol. 119, no. 31, e2122460119,
National Academy of Sciences, 2022, doi:10.1073/pnas.2122460119.
short: R. Abualia, K. Ötvös, O. Novák, E. Bouguyon, K. Domanegg, A. Krapp, P. Nacry,
A. Gojon, B. Lacombe, E. Benková, Proceedings of the National Academy of Sciences
of the United States of America 119 (2022).
corr_author: '1'
date_created: 2022-08-07T22:01:57Z
date_published: 2022-07-25T00:00:00Z
date_updated: 2025-05-14T11:00:29Z
day: '25'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.1073/pnas.2122460119
external_id:
isi:
- '000881496900007'
pmid:
- '35878040'
file:
- access_level: open_access
checksum: 6e97dedc281247fc3fe238a209f14af0
content_type: application/pdf
creator: dernst
date_created: 2022-08-08T07:09:58Z
date_updated: 2022-08-08T07:09:58Z
file_id: '11744'
file_name: 2022_PNAS_Abualia.pdf
file_size: 3092330
relation: main_file
success: 1
file_date_updated: 2022-08-08T07:09:58Z
fulldoi: https://doi.org/10.1073/pnas.2122460119
has_accepted_license: '1'
intvolume: ' 119'
isi: 1
issue: '31'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular framework integrating nitrate sensing in root and auxin-guided shoot
adaptive responses
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 119
year: '2022'
...
---
_id: '10270'
abstract:
- lang: eng
text: Plants develop new organs to adjust their bodies to dynamic changes in the
environment. How independent organs achieve anisotropic shapes and polarities
is poorly understood. To address this question, we constructed a mechano-biochemical
model for Arabidopsis root meristem growth that integrates biologically plausible
principles. Computer model simulations demonstrate how differential growth of
neighboring tissues results in the initial symmetry-breaking leading to anisotropic
root growth. Furthermore, the root growth feeds back on a polar transport network
of the growth regulator auxin. Model, predictions are in close agreement with
in vivo patterns of anisotropic growth, auxin distribution, and cell polarity,
as well as several root phenotypes caused by chemical, mechanical, or genetic
perturbations. Our study demonstrates that the combination of tissue mechanics
and polar auxin transport organizes anisotropic root growth and cell polarities
during organ outgrowth. Therefore, a mobile auxin signal transported through immobile
cells drives polarity and growth mechanics to coordinate complex organ development.
acknowledgement: 'e are grateful Richard Smith, Anne-Lise Routier, Crisanto Gutierrez
and Juergen Kleine-Vehn for providing critical comments on the manuscript. Funding:
This work was supported by the Programa de Atraccion de Talento 2017 (Comunidad
de Madrid, 2017-T1/BIO-5654 to KW), Severo Ochoa (SO) Programme for Centres of Excellence
in R&D from the Agencia Estatal de Investigacion of Spain (grant SEV-2016–0672 (2017–2021)
to KW via the CBGP). In the frame of SEV-2016–0672 funding MM is supported with
a postdoctoral contract. KW was supported by Programa Estatal de Generacion del
Conocimiento y Fortalecimiento Cientıfico y Tecnologico del Sistema de I + D + I
2019 (PGC2018-093387-A-I00) from MICIU (to KW). MG is recipient of an IST Interdisciplinary
Project (IC1022IPC03).'
article_number: '72132'
article_processing_charge: Yes
article_type: original
author:
- first_name: Marco
full_name: Marconi, Marco
last_name: Marconi
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Krzysztof
full_name: Wabnik, Krzysztof
last_name: Wabnik
citation:
ama: Marconi M, Gallemi M, Benková E, Wabnik K. A coupled mechano-biochemical model
for cell polarity guided anisotropic root growth. eLife. 2021;10. doi:10.7554/elife.72132
apa: Marconi, M., Gallemi, M., Benková, E., & Wabnik, K. (2021). A coupled mechano-biochemical
model for cell polarity guided anisotropic root growth. ELife. eLife Sciences
Publications. https://doi.org/10.7554/elife.72132
chicago: Marconi, Marco, Marçal Gallemi, Eva Benková, and Krzysztof Wabnik. “A Coupled
Mechano-Biochemical Model for Cell Polarity Guided Anisotropic Root Growth.” ELife.
eLife Sciences Publications, 2021. https://doi.org/10.7554/elife.72132.
ieee: M. Marconi, M. Gallemi, E. Benková, and K. Wabnik, “A coupled mechano-biochemical
model for cell polarity guided anisotropic root growth,” eLife, vol. 10.
eLife Sciences Publications, 2021.
ista: Marconi M, Gallemi M, Benková E, Wabnik K. 2021. A coupled mechano-biochemical
model for cell polarity guided anisotropic root growth. eLife. 10, 72132.
mla: Marconi, Marco, et al. “A Coupled Mechano-Biochemical Model for Cell Polarity
Guided Anisotropic Root Growth.” ELife, vol. 10, 72132, eLife Sciences
Publications, 2021, doi:10.7554/elife.72132.
short: M. Marconi, M. Gallemi, E. Benková, K. Wabnik, ELife 10 (2021).
date_created: 2021-11-11T10:05:18Z
date_published: 2021-11-01T00:00:00Z
date_updated: 2023-08-14T11:49:23Z
day: '01'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.7554/elife.72132
external_id:
isi:
- '000734671200001'
pmid:
- '34723798'
file:
- access_level: open_access
checksum: fad13c509b53bb7a2bef9c946a7ca60a
content_type: application/pdf
creator: dernst
date_created: 2022-05-13T09:00:29Z
date_updated: 2022-05-13T09:00:29Z
file_id: '11372'
file_name: 2021_eLife_Marconi.pdf
file_size: 14137503
relation: main_file
success: 1
file_date_updated: 2022-05-13T09:00:29Z
fulldoi: https://doi.org/10.7554/elife.72132
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: A coupled mechano-biochemical model for cell polarity guided anisotropic root
growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2021'
...
---
_id: '9212'
abstract:
- lang: eng
text: Plant fitness is largely dependent on the root, the underground organ, which,
besides its anchoring function, supplies the plant body with water and all nutrients
necessary for growth and development. To exploit the soil effectively, roots must
constantly integrate environmental signals and react through adjustment of growth
and development. Important components of the root management strategy involve
a rapid modulation of the root growth kinetics and growth direction, as well as
an increase of the root system radius through formation of lateral roots (LRs).
At the molecular level, such a fascinating growth and developmental flexibility
of root organ requires regulatory networks that guarantee stability of the developmental
program but also allows integration of various environmental inputs. The plant
hormone auxin is one of the principal endogenous regulators of root system architecture
by controlling primary root growth and formation of LR. In this review, we discuss
recent progress in understanding molecular networks where auxin is one of the
main players shaping the root system and acting as mediator between endogenous
cues and environmental factors.
acknowledgement: We apologize to all the authors whose scientific work could not be
cited and discussed because of space restrictions. We thank Dr. Inge Verstraeten
(ISTAustria) and Dr. Juan Carlos Montesinos-Lopez (ETH Zürich) for helpful suggestions.
This work was supported by the DOC Fellowship Programme of the Austrian Academy
of Sciences (25008) to C.A.
article_number: a039941
article_processing_charge: No
article_type: original
author:
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
- first_name: Christina
full_name: Artner, Christina
id: 45DF286A-F248-11E8-B48F-1D18A9856A87
last_name: Artner
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Cavallari N, Artner C, Benková E. Auxin-regulated lateral root organogenesis.
Cold Spring Harbor Perspectives in Biology. 2021;13(7). doi:10.1101/cshperspect.a039941
apa: Cavallari, N., Artner, C., & Benková, E. (2021). Auxin-regulated lateral
root organogenesis. Cold Spring Harbor Perspectives in Biology. Cold Spring
Harbor Laboratory Press. https://doi.org/10.1101/cshperspect.a039941
chicago: Cavallari, Nicola, Christina Artner, and Eva Benková. “Auxin-Regulated
Lateral Root Organogenesis.” Cold Spring Harbor Perspectives in Biology.
Cold Spring Harbor Laboratory Press, 2021. https://doi.org/10.1101/cshperspect.a039941.
ieee: N. Cavallari, C. Artner, and E. Benková, “Auxin-regulated lateral root organogenesis,”
Cold Spring Harbor Perspectives in Biology, vol. 13, no. 7. Cold Spring
Harbor Laboratory Press, 2021.
ista: Cavallari N, Artner C, Benková E. 2021. Auxin-regulated lateral root organogenesis.
Cold Spring Harbor Perspectives in Biology. 13(7), a039941.
mla: Cavallari, Nicola, et al. “Auxin-Regulated Lateral Root Organogenesis.” Cold
Spring Harbor Perspectives in Biology, vol. 13, no. 7, a039941, Cold Spring
Harbor Laboratory Press, 2021, doi:10.1101/cshperspect.a039941.
short: N. Cavallari, C. Artner, E. Benková, Cold Spring Harbor Perspectives in Biology
13 (2021).
corr_author: '1'
date_created: 2021-03-01T10:08:32Z
date_published: 2021-07-01T00:00:00Z
date_updated: 2026-06-18T19:41:34Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.1101/cshperspect.a039941
external_id:
isi:
- '000692069100001'
pmid:
- '33558367'
fulldoi: https://doi.org/10.1101/cshperspect.a039941
intvolume: ' 13'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/cshperspect.a039941
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2685A872-B435-11E9-9278-68D0E5697425
name: Hormonal regulation of plant adaptive responses to environmental signals
publication: Cold Spring Harbor Perspectives in Biology
publication_identifier:
issn:
- 1943-0264
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin-regulated lateral root organogenesis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2021'
...
---
_id: '9332'
abstract:
- lang: eng
text: Lateral root (LR) formation is an example of a plant post-embryonic organogenesis
event. LRs are issued from non-dividing cells entering consecutive steps of formative
divisions, proliferation and elongation. The chromatin remodeling protein PICKLE
(PKL) negatively regulates auxin-mediated LR formation through a mechanism that
is not yet known. Here we show that PKL interacts with RETINOBLASTOMA-RELATED
1 (RBR1) to repress the LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) promoter activity.
Since LBD16 function is required for the formative division of LR founder cells,
repression mediated by the PKL–RBR1 complex negatively regulates formative division
and LR formation. Inhibition of LR formation by PKL–RBR1 is counteracted by auxin,
indicating that, in addition to auxin-mediated transcriptional responses, the
fine-tuned process of LR formation is also controlled at the chromatin level in
an auxin-signaling dependent manner.
acknowledgement: "This research was supported by a postdoctoral fellowship of the
Carl Tryggers Foundation (to K.Ö.) and by grants from Vetenskapsrådet (Nr.: 621-2004-2921
to L.B.) and VINNOVA (to L.B. and S.R.).\r\nWe thank Frederic Berger, Hidehiro Fukaki,
Malcolm Bennett, Claudia Köhler, Jiri Friml for providing pRBR1::RBR1-RFP, ssl2-1,
slr-1, pPKL::PKL-GFP seeds and the DR5 expressing vector, respectively. Authors
are grateful to Hayashi Kenichiro for providing the auxinol compound and to Rishi
Bhalerao for stimulating discussions. The technical help of Adeline Rigal and Thomas
Vain with the auxinol experiments is much appreciated."
article_number: '3862'
article_processing_charge: No
article_type: original
author:
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Pál
full_name: Miskolczi, Pál
last_name: Miskolczi
- first_name: Peter
full_name: Marhavý, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavý
orcid: 0000-0001-5227-5741
- first_name: Alfredo
full_name: Cruz-Ramírez, Alfredo
last_name: Cruz-Ramírez
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: László
full_name: Bakó, László
last_name: Bakó
citation:
ama: Ötvös K, Miskolczi P, Marhavý P, et al. Pickle recruits retinoblastoma related
1 to control lateral root formation in arabidopsis. International Journal of
Molecular Sciences. 2021;22(8). doi:10.3390/ijms22083862
apa: Ötvös, K., Miskolczi, P., Marhavý, P., Cruz-Ramírez, A., Benková, E., Robert,
S., & Bakó, L. (2021). Pickle recruits retinoblastoma related 1 to control
lateral root formation in arabidopsis. International Journal of Molecular Sciences.
MDPI. https://doi.org/10.3390/ijms22083862
chicago: Ötvös, Krisztina, Pál Miskolczi, Peter Marhavý, Alfredo Cruz-Ramírez, Eva
Benková, Stéphanie Robert, and László Bakó. “Pickle Recruits Retinoblastoma Related
1 to Control Lateral Root Formation in Arabidopsis.” International Journal
of Molecular Sciences. MDPI, 2021. https://doi.org/10.3390/ijms22083862.
ieee: K. Ötvös et al., “Pickle recruits retinoblastoma related 1 to control
lateral root formation in arabidopsis,” International Journal of Molecular
Sciences, vol. 22, no. 8. MDPI, 2021.
ista: Ötvös K, Miskolczi P, Marhavý P, Cruz-Ramírez A, Benková E, Robert S, Bakó
L. 2021. Pickle recruits retinoblastoma related 1 to control lateral root formation
in arabidopsis. International Journal of Molecular Sciences. 22(8), 3862.
mla: Ötvös, Krisztina, et al. “Pickle Recruits Retinoblastoma Related 1 to Control
Lateral Root Formation in Arabidopsis.” International Journal of Molecular
Sciences, vol. 22, no. 8, 3862, MDPI, 2021, doi:10.3390/ijms22083862.
short: K. Ötvös, P. Miskolczi, P. Marhavý, A. Cruz-Ramírez, E. Benková, S. Robert,
L. Bakó, International Journal of Molecular Sciences 22 (2021).
date_created: 2021-04-18T22:01:41Z
date_published: 2021-04-08T00:00:00Z
date_updated: 2025-06-12T06:39:41Z
day: '08'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.3390/ijms22083862
external_id:
isi:
- '000644394800001'
pmid:
- '33917959'
file:
- access_level: open_access
checksum: 26ada2531ad1f9c01a1664de0431f1fe
content_type: application/pdf
creator: dernst
date_created: 2021-04-19T10:54:55Z
date_updated: 2021-04-19T10:54:55Z
file_id: '9342'
file_name: 2021_JourMolecularScience_Oetvoes.pdf
file_size: 2769717
relation: main_file
success: 1
file_date_updated: 2021-04-19T10:54:55Z
fulldoi: https://doi.org/10.3390/ijms22083862
has_accepted_license: '1'
intvolume: ' 22'
isi: 1
issue: '8'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
issn:
- 1661-6596
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Pickle recruits retinoblastoma related 1 to control lateral root formation
in arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 22
year: '2021'
...
---
_id: '9986'
abstract:
- lang: eng
text: Size control is a fundamental question in biology, showing incremental complexity
in plants, whose cells possess a rigid cell wall. The phytohormone auxin is a
vital growth regulator with central importance for differential growth control.
Our results indicate that auxin-reliant growth programs affect the molecular complexity
of xyloglucans, the major type of cell wall hemicellulose in eudicots. Auxin-dependent
induction and repression of growth coincide with reduced and enhanced molecular
complexity of xyloglucans, respectively. In agreement with a proposed function
in growth control, genetic interference with xyloglucan side decorations distinctly
modulates auxin-dependent differential growth rates. Our work proposes that auxin-dependent
growth programs have a spatially defined effect on xyloglucan’s molecular structure,
which in turn affects cell wall mechanics and specifies differential, gravitropic
hypocotyl growth.
acknowledgement: "We are grateful to Paul Knox, Markus Pauly, Malcom O’Neill, and
Ignacio Zarra for providing published material; the BOKU-VIBT Imaging Center for
access and M. Debreczeny for expertise; J.I. Thaker and Georg Seifert for critical
reading.\r\n"
article_number: '9222'
article_processing_charge: Yes
article_type: original
author:
- first_name: Silvia Melina
full_name: Velasquez, Silvia Melina
last_name: Velasquez
- first_name: Xiaoyuan
full_name: Guo, Xiaoyuan
last_name: Guo
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Bibek
full_name: Aryal, Bibek
last_name: Aryal
- first_name: Peter
full_name: Venhuizen, Peter
last_name: Venhuizen
- first_name: Elke
full_name: Barbez, Elke
last_name: Barbez
- first_name: Kai Alexander
full_name: Dünser, Kai Alexander
last_name: Dünser
- first_name: Martin
full_name: Darino, Martin
last_name: Darino
- first_name: Aleš
full_name: Pӗnčík, Aleš
last_name: Pӗnčík
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Maria
full_name: Kalyna, Maria
last_name: Kalyna
- first_name: Gregory
full_name: Mouille, Gregory
last_name: Mouille
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Rishikesh P.
full_name: Bhalerao, Rishikesh P.
last_name: Bhalerao
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine-Vehn
citation:
ama: Velasquez SM, Guo X, Gallemi M, et al. Xyloglucan remodeling defines auxin-dependent
differential tissue expansion in plants. International Journal of Molecular
Sciences. 2021;22(17). doi:10.3390/ijms22179222
apa: Velasquez, S. M., Guo, X., Gallemi, M., Aryal, B., Venhuizen, P., Barbez, E.,
… Kleine-Vehn, J. (2021). Xyloglucan remodeling defines auxin-dependent differential
tissue expansion in plants. International Journal of Molecular Sciences.
MDPI. https://doi.org/10.3390/ijms22179222
chicago: Velasquez, Silvia Melina, Xiaoyuan Guo, Marçal Gallemi, Bibek Aryal, Peter
Venhuizen, Elke Barbez, Kai Alexander Dünser, et al. “Xyloglucan Remodeling Defines
Auxin-Dependent Differential Tissue Expansion in Plants.” International Journal
of Molecular Sciences. MDPI, 2021. https://doi.org/10.3390/ijms22179222.
ieee: S. M. Velasquez et al., “Xyloglucan remodeling defines auxin-dependent
differential tissue expansion in plants,” International Journal of Molecular
Sciences, vol. 22, no. 17. MDPI, 2021.
ista: Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA,
Darino M, Pӗnčík A, Novák O, Kalyna M, Mouille G, Benková E, Bhalerao RP, Mravec
J, Kleine-Vehn J. 2021. Xyloglucan remodeling defines auxin-dependent differential
tissue expansion in plants. International Journal of Molecular Sciences. 22(17),
9222.
mla: Velasquez, Silvia Melina, et al. “Xyloglucan Remodeling Defines Auxin-Dependent
Differential Tissue Expansion in Plants.” International Journal of Molecular
Sciences, vol. 22, no. 17, 9222, MDPI, 2021, doi:10.3390/ijms22179222.
short: S.M. Velasquez, X. Guo, M. Gallemi, B. Aryal, P. Venhuizen, E. Barbez, K.A.
Dünser, M. Darino, A. Pӗnčík, O. Novák, M. Kalyna, G. Mouille, E. Benková, R.P.
Bhalerao, J. Mravec, J. Kleine-Vehn, International Journal of Molecular Sciences
22 (2021).
corr_author: '1'
date_created: 2021-09-05T22:01:24Z
date_published: 2021-08-26T00:00:00Z
date_updated: 2024-10-09T21:00:50Z
day: '26'
ddc:
- '575'
department:
- _id: EvBe
doi: 10.3390/ijms22179222
external_id:
isi:
- '000694347100001'
pmid:
- '34502129'
file:
- access_level: open_access
checksum: 6b7055cf89f1b7ed8594c3fdf56f000b
content_type: application/pdf
creator: cchlebak
date_created: 2021-09-06T12:50:19Z
date_updated: 2021-09-07T09:04:53Z
file_id: '9988'
file_name: 2021_IntJMolecularSciences_Velasquez.pdf
file_size: 2162247
relation: main_file
file_date_updated: 2021-09-07T09:04:53Z
fulldoi: https://doi.org/10.3390/ijms22179222
has_accepted_license: '1'
intvolume: ' 22'
isi: 1
issue: '17'
keyword:
- auxin
- growth
- cell wall
- xyloglucans
- hypocotyls
- gravitropism
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
issn:
- 1661-6596
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Xyloglucan remodeling defines auxin-dependent differential tissue expansion
in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 22
year: '2021'
...
---
_id: '9010'
abstract:
- lang: eng
text: Availability of the essential macronutrient nitrogen in soil plays a critical
role in plant growth, development, and impacts agricultural productivity. Plants
have evolved different strategies for sensing and responding to heterogeneous
nitrogen distribution. Modulation of root system architecture, including primary
root growth and branching, is among the most essential plant adaptions to ensure
adequate nitrogen acquisition. However, the immediate molecular pathways coordinating
the adjustment of root growth in response to distinct nitrogen sources, such as
nitrate or ammonium, are poorly understood. Here, we show that growth as manifested
by cell division and elongation is synchronized by coordinated auxin flux between
two adjacent outer tissue layers of the root. This coordination is achieved by
nitrate‐dependent dephosphorylation of the PIN2 auxin efflux carrier at a previously
uncharacterized phosphorylation site, leading to subsequent PIN2 lateralization
and thereby regulating auxin flow between adjacent tissues. A dynamic computer
model based on our experimental data successfully recapitulates experimental observations.
Our study provides mechanistic insights broadening our understanding of root growth
mechanisms in dynamic environments.
acknowledged_ssus:
- _id: Bio
acknowledgement: 'We acknowledge Gergely Molnar for critical reading of the manuscript,
Alexander Johnson for language editing and Yulija Salanenka for technical assistance.
Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S)
to KO, RA and EB. Work in the Benkova laboratory was supported by the Austrian Science
Fund (FWF01_I1774S) to KO, RA and EB and by the DOC Fellowship Programme of the
AustrianAcademy of Sciences (25008) to C.A. Work in the Wabnik laboratory was supported
by the Programa de Atraccion de Talento 2017 (Comunidad deMadrid, 2017-T1/BIO-5654
to K.W.), Severo Ochoa Programme for Centres of Excellence in R&D from the Agencia
Estatal de Investigacion of Spain (grantSEV-2016-0672 (2017-2021) to K.W. via the
CBGP) and Programa Estatal de Generacion del Conocimiento y Fortalecimiento Científico
y Tecnologico del Sistema de I+D+I 2019 (PGC2018-093387-A-I00) from MICIU (to K.W.).
M.M.was supported by a postdoctoral contract associated to SEV-2016-0672.We acknowledge
the Bioimaging Facility in IST-Austria and the Advanced Microscopy Facility of the
Vienna Bio Center Core Facilities, member of the Vienna Bio Center Austria, for
use of the OMX v43D SIM microscope. AJ was supported by the Austrian Science Fund
(FWF): I03630 to J.F'
article_number: e106862
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Marco
full_name: Marconi, Marco
last_name: Marconi
- first_name: Andrea
full_name: Vega, Andrea
last_name: Vega
- first_name: Jose
full_name: O’Brien, Jose
last_name: O’Brien
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Livio
full_name: Antonielli, Livio
last_name: Antonielli
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Christina
full_name: Artner, Christina
id: 45DF286A-F248-11E8-B48F-1D18A9856A87
last_name: Artner
- first_name: Eleonore
full_name: Bouguyon, Eleonore
last_name: Bouguyon
- first_name: Alain
full_name: Gojon, Alain
last_name: Gojon
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Rodrigo A.
full_name: Gutiérrez, Rodrigo A.
last_name: Gutiérrez
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Ötvös K, Marconi M, Vega A, et al. Modulation of plant root growth by nitrogen
source-defined regulation of polar auxin transport. EMBO Journal. 2021;40(3).
doi:10.15252/embj.2020106862
apa: Ötvös, K., Marconi, M., Vega, A., O’Brien, J., Johnson, A. J., Abualia, R.,
… Benková, E. (2021). Modulation of plant root growth by nitrogen source-defined
regulation of polar auxin transport. EMBO Journal. Embo Press. https://doi.org/10.15252/embj.2020106862
chicago: Ötvös, Krisztina, Marco Marconi, Andrea Vega, Jose O’Brien, Alexander J
Johnson, Rashed Abualia, Livio Antonielli, et al. “Modulation of Plant Root Growth
by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” EMBO Journal.
Embo Press, 2021. https://doi.org/10.15252/embj.2020106862.
ieee: K. Ötvös et al., “Modulation of plant root growth by nitrogen source-defined
regulation of polar auxin transport,” EMBO Journal, vol. 40, no. 3. Embo
Press, 2021.
ista: Ötvös K, Marconi M, Vega A, O’Brien J, Johnson AJ, Abualia R, Antonielli L,
Montesinos López JC, Zhang Y, Tan S, Cuesta C, Artner C, Bouguyon E, Gojon A,
Friml J, Gutiérrez RA, Wabnik KT, Benková E. 2021. Modulation of plant root growth
by nitrogen source-defined regulation of polar auxin transport. EMBO Journal.
40(3), e106862.
mla: Ötvös, Krisztina, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined
Regulation of Polar Auxin Transport.” EMBO Journal, vol. 40, no. 3, e106862,
Embo Press, 2021, doi:10.15252/embj.2020106862.
short: K. Ötvös, M. Marconi, A. Vega, J. O’Brien, A.J. Johnson, R. Abualia, L. Antonielli,
J.C. Montesinos López, Y. Zhang, S. Tan, C. Cuesta, C. Artner, E. Bouguyon, A.
Gojon, J. Friml, R.A. Gutiérrez, K.T. Wabnik, E. Benková, EMBO Journal 40 (2021).
corr_author: '1'
date_created: 2021-01-17T23:01:12Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2026-10-15T22:30:51Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.15252/embj.2020106862
external_id:
isi:
- '000604645600001'
pmid:
- ' 33399250'
file:
- access_level: open_access
checksum: dc55c900f3b061d6c2790b8813d759a3
content_type: application/pdf
creator: dernst
date_created: 2021-02-11T12:28:29Z
date_updated: 2021-02-11T12:28:29Z
file_id: '9110'
file_name: 2021_Embo_Otvos.pdf
file_size: 2358617
relation: main_file
success: 1
file_date_updated: 2021-02-11T12:28:29Z
fulldoi: https://doi.org/10.15252/embj.2020106862
has_accepted_license: '1'
intvolume: ' 40'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
- _id: 2685A872-B435-11E9-9278-68D0E5697425
name: Hormonal regulation of plant adaptive responses to environmental signals
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/a-plants-way-to-its-favorite-food/
record:
- id: '10303'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Modulation of plant root growth by nitrogen source-defined regulation of polar
auxin transport
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 40
year: '2021'
...
---
_id: '9913'
abstract:
- lang: eng
text: Nitrate commands genome-wide gene expression changes that impact metabolism,
physiology, plant growth, and development. In an effort to identify new components
involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root
phosphoproteome in response to nitrate treatments via liquid chromatography coupled
to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5
or 20 min after nitrate treatments. Proteins identified by 5 min include signaling
components such as kinases or transcription factors. In contrast, by 20 min, proteins
identified were associated with transporter activity or hormone metabolism functions,
among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1)
mutant plants was significantly altered as compared to wild-type plants, confirming
its key role in nitrate signaling pathways that involves phosphorylation changes.
Integrative bioinformatics analysis highlights auxin transport as an important
mechanism modulated by nitrate signaling at the post-translational level. We validated
a new phosphorylation site in PIN2 and provide evidence that it functions in primary
and lateral root growth responses to nitrate.
acknowledgement: This work was supported by ANID—Millennium Science Initiative Program—ICN17_022,
Fondo de Desarrollo de Areas Prioritarias (FONDAP) Center for Genome Regulation
(15090007), ANID—Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT)
1180759 (to RAG) and 1171631 (to AV). We would like to thank Unidad de Microscopía
Avanzada UC (UMA UC).
article_number: e51813
article_processing_charge: Yes
article_type: original
author:
- first_name: Andrea
full_name: Vega, Andrea
last_name: Vega
- first_name: Isabel
full_name: Fredes, Isabel
last_name: Fredes
- first_name: José
full_name: O’Brien, José
last_name: O’Brien
- first_name: Zhouxin
full_name: Shen, Zhouxin
last_name: Shen
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Steven P.
full_name: Briggs, Steven P.
last_name: Briggs
- first_name: Rodrigo A.
full_name: Gutiérrez, Rodrigo A.
last_name: Gutiérrez
citation:
ama: Vega A, Fredes I, O’Brien J, et al. Nitrate triggered phosphoproteome changes
and a PIN2 phosphosite modulating root system architecture. EMBO Reports.
2021;22(9). doi:10.15252/embr.202051813
apa: Vega, A., Fredes, I., O’Brien, J., Shen, Z., Ötvös, K., Abualia, R., … Gutiérrez,
R. A. (2021). Nitrate triggered phosphoproteome changes and a PIN2 phosphosite
modulating root system architecture. EMBO Reports. Wiley. https://doi.org/10.15252/embr.202051813
chicago: Vega, Andrea, Isabel Fredes, José O’Brien, Zhouxin Shen, Krisztina Ötvös,
Rashed Abualia, Eva Benková, Steven P. Briggs, and Rodrigo A. Gutiérrez. “Nitrate
Triggered Phosphoproteome Changes and a PIN2 Phosphosite Modulating Root System
Architecture.” EMBO Reports. Wiley, 2021. https://doi.org/10.15252/embr.202051813.
ieee: A. Vega et al., “Nitrate triggered phosphoproteome changes and a PIN2
phosphosite modulating root system architecture,” EMBO Reports, vol. 22,
no. 9. Wiley, 2021.
ista: Vega A, Fredes I, O’Brien J, Shen Z, Ötvös K, Abualia R, Benková E, Briggs
SP, Gutiérrez RA. 2021. Nitrate triggered phosphoproteome changes and a PIN2 phosphosite
modulating root system architecture. EMBO Reports. 22(9), e51813.
mla: Vega, Andrea, et al. “Nitrate Triggered Phosphoproteome Changes and a PIN2
Phosphosite Modulating Root System Architecture.” EMBO Reports, vol. 22,
no. 9, e51813, Wiley, 2021, doi:10.15252/embr.202051813.
short: A. Vega, I. Fredes, J. O’Brien, Z. Shen, K. Ötvös, R. Abualia, E. Benková,
S.P. Briggs, R.A. Gutiérrez, EMBO Reports 22 (2021).
date_created: 2021-08-15T22:01:30Z
date_published: 2021-09-06T00:00:00Z
date_updated: 2026-10-15T22:30:51Z
day: '06'
ddc:
- '580'
department:
- _id: EvBe
- _id: GradSch
doi: 10.15252/embr.202051813
external_id:
isi:
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pmid:
- '34357701 '
file:
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creator: cchlebak
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isi: 1
issue: '9'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: EMBO Reports
publication_identifier:
eissn:
- 1469-3178
issn:
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publication_status: published
publisher: Wiley
quality_controlled: '1'
related_material:
record:
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relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating
root system architecture
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 22
year: '2021'
...
---
_id: '7805'
abstract:
- lang: eng
text: Plants as non-mobile organisms constantly integrate varying environmental
signals to flexibly adapt their growth and development. Local fluctuations in
water and nutrient availability, sudden changes in temperature or other abiotic
and biotic stresses can trigger changes in the growth of plant organs. Multiple
mutually interconnected hormonal signaling cascades act as essential endogenous
translators of these exogenous signals in the adaptive responses of plants. Although
the molecular backbones of hormone transduction pathways have been identified,
the mechanisms underlying their interactions are largely unknown. Here, using
genome wide transcriptome profiling we identify an auxin and cytokinin cross-talk
component; SYNERGISTIC ON AUXIN AND CYTOKININ 1 (SYAC1), whose expression in roots
is strictly dependent on both of these hormonal pathways. We show that SYAC1 is
a regulator of secretory pathway, whose enhanced activity interferes with deposition
of cell wall components and can fine-tune organ growth and sensitivity to soil
pathogens.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We thank Daria Siekhaus, Jiri Friml and Alexander Johnson for critical
reading of the manuscript, Peter Pimpl, Christian Luschnig and Liwen Jiang for sharing
published material, Lesia Rodriguez Solovey for technical assistance. This work
was supported by the Austrian Science Fund (FWF01_I1774S) to A.H., K.Ö., and E.B.,
the German Research Foundation (DFG; He3424/6-1 to I.H.), by the People Programme
(Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013)
under REA grant agreement n° [291734] (to N.C.), by the EU in the framework of the
Marie-Curie FP7 COFUND People Programme through the award of an AgreenSkills+ fellowship
No. 609398 (to J.S.) and by the Scientific Service Units of IST-Austria through
resources provided by the Bioimaging Facility, the Life Science Facility. The IJPB
benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).
article_number: '2170'
article_processing_charge: No
article_type: original
author:
- first_name: Andrej
full_name: Hurny, Andrej
id: 4DC4AF46-F248-11E8-B48F-1D18A9856A87
last_name: Hurny
orcid: 0000-0003-3638-1426
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Jerome
full_name: Duclercq, Jerome
last_name: Duclercq
- first_name: Ladislav
full_name: Dokládal, Ladislav
last_name: Dokládal
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Hana
full_name: Semeradova, Hana
id: 42FE702E-F248-11E8-B48F-1D18A9856A87
last_name: Semeradova
- first_name: Thomas
full_name: Rauter, Thomas
id: A0385D1A-9376-11EA-A47D-9862C5E3AB22
last_name: Rauter
- first_name: Irene
full_name: Stenzel, Irene
last_name: Stenzel
- first_name: Geert
full_name: Persiau, Geert
last_name: Persiau
- first_name: Freia
full_name: Benade, Freia
last_name: Benade
- first_name: Rishikesh
full_name: Bhalearo, Rishikesh
last_name: Bhalearo
- first_name: Eva
full_name: Sýkorová, Eva
last_name: Sýkorová
- first_name: András
full_name: Gorzsás, András
last_name: Gorzsás
- first_name: Julien
full_name: Sechet, Julien
last_name: Sechet
- first_name: Gregory
full_name: Mouille, Gregory
last_name: Mouille
- first_name: Ingo
full_name: Heilmann, Ingo
last_name: Heilmann
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Jutta
full_name: Ludwig-Müller, Jutta
last_name: Ludwig-Müller
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Hurny A, Cuesta C, Cavallari N, et al. Synergistic on Auxin and Cytokinin 1
positively regulates growth and attenuates soil pathogen resistance. Nature
Communications. 2020;11. doi:10.1038/s41467-020-15895-5
apa: Hurny, A., Cuesta, C., Cavallari, N., Ötvös, K., Duclercq, J., Dokládal, L.,
… Benková, E. (2020). Synergistic on Auxin and Cytokinin 1 positively regulates
growth and attenuates soil pathogen resistance. Nature Communications.
Springer Nature. https://doi.org/10.1038/s41467-020-15895-5
chicago: Hurny, Andrej, Candela Cuesta, Nicola Cavallari, Krisztina Ötvös, Jerome
Duclercq, Ladislav Dokládal, Juan C Montesinos López, et al. “Synergistic on Auxin
and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.”
Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-15895-5.
ieee: A. Hurny et al., “Synergistic on Auxin and Cytokinin 1 positively regulates
growth and attenuates soil pathogen resistance,” Nature Communications,
vol. 11. Springer Nature, 2020.
ista: Hurny A, Cuesta C, Cavallari N, Ötvös K, Duclercq J, Dokládal L, Montesinos
López JC, Gallemi M, Semerádová H, Rauter T, Stenzel I, Persiau G, Benade F, Bhalearo
R, Sýkorová E, Gorzsás A, Sechet J, Mouille G, Heilmann I, De Jaeger G, Ludwig-Müller
J, Benková E. 2020. Synergistic on Auxin and Cytokinin 1 positively regulates
growth and attenuates soil pathogen resistance. Nature Communications. 11, 2170.
mla: Hurny, Andrej, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates
Growth and Attenuates Soil Pathogen Resistance.” Nature Communications,
vol. 11, 2170, Springer Nature, 2020, doi:10.1038/s41467-020-15895-5.
short: A. Hurny, C. Cuesta, N. Cavallari, K. Ötvös, J. Duclercq, L. Dokládal, J.C.
Montesinos López, M. Gallemi, H. Semerádová, T. Rauter, I. Stenzel, G. Persiau,
F. Benade, R. Bhalearo, E. Sýkorová, A. Gorzsás, J. Sechet, G. Mouille, I. Heilmann,
G. De Jaeger, J. Ludwig-Müller, E. Benková, Nature Communications 11 (2020).
corr_author: '1'
date_created: 2020-05-10T22:00:48Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2026-04-02T14:32:53Z
day: '01'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.1038/s41467-020-15895-5
ec_funded: 1
external_id:
isi:
- '000531425900012'
pmid:
- '32358503'
file:
- access_level: open_access
checksum: 2cba327c9e9416d75cb96be54b0fb441
content_type: application/pdf
creator: dernst
date_created: 2020-10-06T07:47:53Z
date_updated: 2020-10-06T07:47:53Z
file_id: '8614'
file_name: 2020_NatureComm_Hurny.pdf
file_size: 4743576
relation: main_file
success: 1
file_date_updated: 2020-10-06T07:47:53Z
fulldoi: https://doi.org/10.1038/s41467-020-15895-5
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates
soil pathogen resistance
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 11
year: '2020'
...