[{"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"doi":"10.1016/j.cub.2023.11.067","project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"}],"quality_controlled":"1","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"month":"01","volume":34,"date_created":"2024-01-14T23:00:56Z","date_updated":"2024-01-17T08:20:40Z","author":[{"orcid":"0000-0001-5809-9566","id":"49DA7910-F248-11E8-B48F-1D18A9856A87","last_name":"Arslan","first_name":"Feyza N","full_name":"Arslan, Feyza N"},{"last_name":"Hannezo","first_name":"Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"},{"full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Loose","first_name":"Martin","orcid":"0000-0001-7309-9724","id":"462D4284-F248-11E8-B48F-1D18A9856A87","full_name":"Loose, Martin"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"NanoFab"}],"publisher":"Elsevier","publication_status":"published","acknowledgement":"We are grateful to Edwin Munro for their feedback and help with the single particle analysis. We thank members of the Heisenberg and Loose labs for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA for their continuous support, especially Yann Cesbron for assistance with the laser cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H.","year":"2024","license":"https://creativecommons.org/licenses/by/4.0/","ec_funded":1,"file_date_updated":"2024-01-16T10:53:31Z","date_published":"2024-01-08T00:00:00Z","page":"171-182.e8","article_type":"original","citation":{"chicago":"Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” Current Biology. Elsevier, 2024. https://doi.org/10.1016/j.cub.2023.11.067.","short":"F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current Biology 34 (2024) 171–182.e8.","mla":"Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” Current Biology, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8, doi:10.1016/j.cub.2023.11.067.","ieee":"F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg, “Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” Current Biology, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.","apa":"Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., & Heisenberg, C.-P. J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2023.11.067","ista":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1), 171–182.e8.","ama":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 2024;34(1):171-182.e8. doi:10.1016/j.cub.2023.11.067"},"publication":"Current Biology","has_accepted_license":"1","article_processing_charge":"Yes (via OA deal)","day":"08","scopus_import":"1","oa_version":"Published Version","file":[{"file_id":"14813","relation":"main_file","success":1,"checksum":"51220b76d72a614208f84bdbfbaf9b72","date_updated":"2024-01-16T10:53:31Z","date_created":"2024-01-16T10:53:31Z","access_level":"open_access","file_name":"2024_CurrentBiology_Arslan.pdf","creator":"dernst","file_size":5183861,"content_type":"application/pdf"}],"intvolume":" 34","ddc":["570"],"title":"Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts","status":"public","_id":"14795","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"1","abstract":[{"text":"Metazoan development relies on the formation and remodeling of cell-cell contacts. Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in space and time plays a central role in cell-cell contact formation and maturation. Nevertheless, how this process is mechanistically achieved when new contacts are formed remains unclear. Here, by building a biomimetic assay composed of progenitor cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains, we show that cortical F-actin flows, driven by the depletion of myosin-2 at the cell contact center, mediate the dynamic reorganization of adhesion receptors and cell cortex at the contact. E-cadherin-dependent downregulation of the small GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2 becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical tension gradient from the contact rim to its center. This tension gradient, in turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin at the contact rim and the progressive redistribution of E-cadherin from the contact center to the rim. Eventually, this combination of actomyosin downregulation and flows at the contact determines the characteristic molecular organization, with E-cadherin and F-actin accumulating at the contact rim, where they are needed to mechanically link the contractile cortices of the adhering cells.","lang":"eng"}],"type":"journal_article"},{"publication_identifier":{"issn":["0950-1991"],"eissn":["1477-9129"]},"month":"02","doi":"10.1242/dev.202316","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"project":[{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"_id":"26B1E39C-B435-11E9-9278-68D0E5697425","grant_number":"25239","name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues"}],"quality_controlled":"1","ec_funded":1,"file_date_updated":"2024-03-04T07:24:43Z","related_material":{"record":[{"status":"public","relation":"research_data","id":"14926"}]},"author":[{"full_name":"Schauer, Alexandra","orcid":"0000-0001-7659-9142","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","last_name":"Schauer","first_name":"Alexandra"},{"id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija","last_name":"Pranjic-Ferscha","full_name":"Pranjic-Ferscha, Kornelija"},{"full_name":"Hauschild, Robert","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"volume":151,"date_created":"2024-03-03T23:00:50Z","date_updated":"2024-03-04T07:28:25Z","year":"2024","acknowledgement":"We thank Patrick Müller for sharing the chordintt250 mutant zebrafish line as well as the plasmid for chrd-GFP, Katherine Rogers for sharing the bmp2b plasmid and Andrea Pauli for sharing the draculin plasmid. Diana Pinheiro generated the MZlefty1,2;Tg(sebox::EGFP) line. We are grateful to Patrick Müller, Diana Pinheiro and Katherine Rogers and members of the Heisenberg lab for discussions, technical advice and feedback on the manuscript. We also thank Anna Kicheva and Edouard Hannezo for discussions. We thank the Imaging and Optics Facility as well as the Life Science facility at IST Austria for support with microscopy and fish maintenance.\r\nThis work was supported by a European Research Council Advanced Grant\r\n(MECSPEC 742573 to C.-P.H.). A.S. is a recipient of a DOC Fellowship of the Austrian\r\nAcademy of Sciences at IST Austria. Open Access funding provided by Institute of\r\nScience and Technology Austria. ","publisher":"The Company of Biologists","department":[{"_id":"CaHe"},{"_id":"Bio"}],"publication_status":"published","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","day":"01","scopus_import":"1","date_published":"2024-02-01T00:00:00Z","citation":{"ama":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. Robust axis elongation by Nodal-dependent restriction of BMP signaling. Development. 2024;151(4):1-18. doi:10.1242/dev.202316","ieee":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, and C.-P. J. Heisenberg, “Robust axis elongation by Nodal-dependent restriction of BMP signaling,” Development, vol. 151, no. 4. The Company of Biologists, pp. 1–18, 2024.","apa":"Schauer, A., Pranjic-Ferscha, K., Hauschild, R., & Heisenberg, C.-P. J. (2024). Robust axis elongation by Nodal-dependent restriction of BMP signaling. Development. The Company of Biologists. https://doi.org/10.1242/dev.202316","ista":"Schauer A, Pranjic-Ferscha K, Hauschild R, Heisenberg C-PJ. 2024. Robust axis elongation by Nodal-dependent restriction of BMP signaling. Development. 151(4), 1–18.","short":"A. Schauer, K. Pranjic-Ferscha, R. Hauschild, C.-P.J. Heisenberg, Development 151 (2024) 1–18.","mla":"Schauer, Alexandra, et al. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” Development, vol. 151, no. 4, The Company of Biologists, 2024, pp. 1–18, doi:10.1242/dev.202316.","chicago":"Schauer, Alexandra, Kornelija Pranjic-Ferscha, Robert Hauschild, and Carl-Philipp J Heisenberg. “Robust Axis Elongation by Nodal-Dependent Restriction of BMP Signaling.” Development. The Company of Biologists, 2024. https://doi.org/10.1242/dev.202316."},"publication":"Development","page":"1-18","article_type":"original","issue":"4","abstract":[{"lang":"eng","text":"Embryogenesis results from the coordinated activities of different signaling pathways controlling cell fate specification and morphogenesis. In vertebrate gastrulation, both Nodal and BMP signaling play key roles in germ layer specification and morphogenesis, yet their interplay to coordinate embryo patterning with morphogenesis is still insufficiently understood. Here, we took a reductionist approach using zebrafish embryonic explants to study the coordination of Nodal and BMP signaling for embryo patterning and morphogenesis. We show that Nodal signaling triggers explant elongation by inducing mesendodermal progenitors but also suppressing BMP signaling activity at the site of mesendoderm induction. Consistent with this, ectopic BMP signaling in the mesendoderm blocks cell alignment and oriented mesendoderm intercalations, key processes during explant elongation. Translating these ex vivo observations to the intact embryo showed that, similar to explants, Nodal signaling suppresses the effect of BMP signaling on cell intercalations in the dorsal domain, thus allowing robust embryonic axis elongation. These findings suggest a dual function of Nodal signaling in embryonic axis elongation by both inducing mesendoderm and suppressing BMP effects in the dorsal portion of the mesendoderm."}],"type":"journal_article","oa_version":"Published Version","file":[{"success":1,"checksum":"6961ea10012bf0d266681f9628bb8f13","date_created":"2024-03-04T07:24:43Z","date_updated":"2024-03-04T07:24:43Z","file_id":"15050","relation":"main_file","creator":"dernst","content_type":"application/pdf","file_size":14839986,"access_level":"open_access","file_name":"2024_Development_Schauer.pdf"}],"_id":"15048","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":" 151","ddc":["570"],"title":"Robust axis elongation by Nodal-dependent restriction of BMP signaling","status":"public"},{"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"main_file_link":[{"url":"https://doi.org/10.1038/s41567-023-02302-1","open_access":"1"}],"quality_controlled":"1","project":[{"grant_number":"I03601","_id":"2646861A-B435-11E9-9278-68D0E5697425","name":"Control of embryonic cleavage pattern","call_identifier":"FWF"}],"doi":"10.1038/s41567-023-02302-1","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"NanoFab"}],"language":[{"iso":"eng"}],"month":"01","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"year":"2024","acknowledgement":"We would like to thank A. McDougall, E. Hannezo and the Heisenberg lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific Service Units of the Institute of Science and Technology Austria through resources provided by the Electron Microscopy Facility, Imaging and Optics Facility and the Nanofabrication Facility. This work was supported by a Joint Project Grant from the FWF (I 3601-B27).","publication_status":"epub_ahead","department":[{"_id":"CaHe"},{"_id":"JoFi"},{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"publisher":"Springer Nature","author":[{"full_name":"Caballero Mancebo, Silvia","first_name":"Silvia","last_name":"Caballero Mancebo","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5223-3346"},{"first_name":"Rushikesh","last_name":"Shinde","full_name":"Shinde, Rushikesh"},{"full_name":"Bolger-Munro, Madison","first_name":"Madison","last_name":"Bolger-Munro","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","orcid":"0000-0002-8176-4824"},{"last_name":"Peruzzo","first_name":"Matilda","orcid":"0000-0002-3415-4628","id":"3F920B30-F248-11E8-B48F-1D18A9856A87","full_name":"Peruzzo, Matilda"},{"last_name":"Szep","first_name":"Gregory","id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","full_name":"Szep, Gregory"},{"id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene","last_name":"Steccari","full_name":"Steccari, Irene"},{"last_name":"Labrousse Arias","first_name":"David","id":"CD573DF4-9ED3-11E9-9D77-3223E6697425","full_name":"Labrousse Arias, David"},{"full_name":"Zheden, Vanessa","orcid":"0000-0002-9438-4783","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","last_name":"Zheden","first_name":"Vanessa"},{"full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","first_name":"Jack","last_name":"Merrin"},{"full_name":"Callan-Jones, Andrew","first_name":"Andrew","last_name":"Callan-Jones"},{"first_name":"Raphaël","last_name":"Voituriez","full_name":"Voituriez, Raphaël"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"link":[{"description":"News on ISTA Website","relation":"press_release","url":"https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/"}]},"date_created":"2024-01-21T23:00:57Z","date_updated":"2024-03-05T09:33:38Z","publication":"Nature Physics","citation":{"chicago":"Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” Nature Physics. Springer Nature, 2024. https://doi.org/10.1038/s41567-023-02302-1.","short":"S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I. Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez, C.-P.J. Heisenberg, Nature Physics (2024).","mla":"Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes upon Fertilization.” Nature Physics, Springer Nature, 2024, doi:10.1038/s41567-023-02302-1.","apa":"Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G., Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-023-02302-1","ieee":"S. Caballero Mancebo et al., “Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization,” Nature Physics. Springer Nature, 2024.","ista":"Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics.","ama":"Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization. Nature Physics. 2024. doi:10.1038/s41567-023-02302-1"},"article_type":"original","date_published":"2024-01-09T00:00:00Z","scopus_import":"1","day":"09","has_accepted_license":"1","article_processing_charge":"Yes (in subscription journal)","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"14846","title":"Friction forces determine cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization","status":"public","oa_version":"Published Version","type":"journal_article","abstract":[{"lang":"eng","text":"Contraction and flow of the actin cell cortex have emerged as a common principle by which cells reorganize their cytoplasm and take shape. However, how these cortical flows interact with adjacent cytoplasmic components, changing their form and localization, and how this affects cytoplasmic organization and cell shape remains unclear. Here we show that in ascidian oocytes, the cooperative activities of cortical actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive oocyte cytoplasmic reorganization and shape changes following fertilization. We show that vegetal-directed cortical actomyosin flows, established upon oocyte fertilization, lead to both the accumulation of cortical actin at the vegetal pole of the zygote and compression and local buckling of the adjacent elastic solid-like myoplasm layer due to friction forces generated at their interface. Once cortical flows have ceased, the multiple myoplasm buckles resolve into one larger buckle, which again drives the formation of the contraction pole—a protuberance of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings reveal a mechanism where cortical actomyosin network flows determine cytoplasmic reorganization and cell shape by deforming adjacent cytoplasmic components through friction forces."}]},{"month":"04","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000982111800001"]},"oa":1,"isi":1,"quality_controlled":"1","project":[{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"grant_number":"ALTF 850-2017","_id":"26520D1E-B435-11E9-9278-68D0E5697425","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation"},{"name":"Coordination of mesendoderm fate specification and internalization during zebrafish gastrulation","_id":"266BC5CE-B435-11E9-9278-68D0E5697425","grant_number":"LT000429"}],"doi":"10.1016/j.devcel.2023.02.016","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"file_date_updated":"2023-04-17T07:41:25Z","ec_funded":1,"year":"2023","acknowledgement":"We thank Andrea Pauli (IMP) and Edouard Hannezo (ISTA) for fruitful discussions and support with the SPIM experiments; the Heisenberg group, and especially Feyza Nur Arslan and Alexandra Schauer, for discussions and feedback; Michaela Jović (ISTA) for help with the quantitative real-time PCR protocol; the bioimaging and zebrafish facilities of ISTA for continuous support; Stephan Preibisch (Janelia Research Campus) for support with the SPIM data analysis; and Nobuhiro Nakamura (Tokyo Institute of Technology) for sharing α1-Na+/K+-ATPase antibody. This work was supported by funding from the European Union (European Research Council Advanced grant 742573 to C.-P.H.), postdoctoral fellowships from EMBO (LTF-850-2017) and HFSP (LT000429/2018-L2) to D.P., and a PhD fellowship from the Studienstiftung des deutschen Volkes to F.P.","publication_status":"published","publisher":"Elsevier","department":[{"_id":"CaHe"},{"_id":"Bio"}],"author":[{"last_name":"Huljev","first_name":"Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","full_name":"Huljev, Karla"},{"id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan","last_name":"Shamipour","full_name":"Shamipour, Shayan"},{"full_name":"Nunes Pinheiro, Diana C","first_name":"Diana C","last_name":"Nunes Pinheiro","id":"2E839F16-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4333-7503"},{"last_name":"Preusser","first_name":"Friedrich","full_name":"Preusser, Friedrich"},{"first_name":"Irene","last_name":"Steccari","id":"2705C766-9FE2-11EA-B224-C6773DDC885E","full_name":"Steccari, Irene"},{"orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","first_name":"Christoph M","full_name":"Sommer, Christoph M"},{"last_name":"Naik","first_name":"Suyash","orcid":"0000-0001-8421-5508","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","full_name":"Naik, Suyash"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"date_updated":"2023-08-01T14:10:38Z","date_created":"2023-04-16T22:01:07Z","volume":58,"scopus_import":"1","day":"10","has_accepted_license":"1","article_processing_charge":"Yes (via OA deal)","publication":"Developmental Cell","citation":{"short":"K. Huljev, S. Shamipour, D.C. Nunes Pinheiro, F. Preusser, I. Steccari, C.M. Sommer, S. Naik, C.-P.J. Heisenberg, Developmental Cell 58 (2023) 582–596.e7.","mla":"Huljev, Karla, et al. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” Developmental Cell, vol. 58, no. 7, Elsevier, 2023, p. 582–596.e7, doi:10.1016/j.devcel.2023.02.016.","chicago":"Huljev, Karla, Shayan Shamipour, Diana C Nunes Pinheiro, Friedrich Preusser, Irene Steccari, Christoph M Sommer, Suyash Naik, and Carl-Philipp J Heisenberg. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” Developmental Cell. Elsevier, 2023. https://doi.org/10.1016/j.devcel.2023.02.016.","ama":"Huljev K, Shamipour S, Nunes Pinheiro DC, et al. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. 2023;58(7):582-596.e7. doi:10.1016/j.devcel.2023.02.016","apa":"Huljev, K., Shamipour, S., Nunes Pinheiro, D. C., Preusser, F., Steccari, I., Sommer, C. M., … Heisenberg, C.-P. J. (2023). A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2023.02.016","ieee":"K. Huljev et al., “A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish,” Developmental Cell, vol. 58, no. 7. Elsevier, p. 582–596.e7, 2023.","ista":"Huljev K, Shamipour S, Nunes Pinheiro DC, Preusser F, Steccari I, Sommer CM, Naik S, Heisenberg C-PJ. 2023. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. 58(7), 582–596.e7."},"article_type":"original","page":"582-596.e7","date_published":"2023-04-10T00:00:00Z","type":"journal_article","abstract":[{"text":"Interstitial fluid (IF) accumulation between embryonic cells is thought to be important for embryo patterning and morphogenesis. Here, we identify a positive mechanical feedback loop between cell migration and IF relocalization and find that it promotes embryonic axis formation during zebrafish gastrulation. We show that anterior axial mesendoderm (prechordal plate [ppl]) cells, moving in between the yolk cell and deep cell tissue to extend the embryonic axis, compress the overlying deep cell layer, thereby causing IF to flow from the deep cell layer to the boundary between the yolk cell and the deep cell layer, directly ahead of the advancing ppl. This IF relocalization, in turn, facilitates ppl cell protrusion formation and migration by opening up the space into which the ppl moves and, thereby, the ability of the ppl to trigger IF relocalization by pushing against the overlying deep cell layer. Thus, embryonic axis formation relies on a hydraulic feedback loop between cell migration and IF relocalization.","lang":"eng"}],"issue":"7","_id":"12830","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","ddc":["570"],"status":"public","title":"A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish","intvolume":" 58","oa_version":"Published Version","file":[{"relation":"main_file","file_id":"12842","date_created":"2023-04-17T07:41:25Z","date_updated":"2023-04-17T07:41:25Z","checksum":"c80ca2ebc241232aacdb5aa4b4c80957","success":1,"file_name":"2023_DevelopmentalCell_Huljev.pdf","access_level":"open_access","content_type":"application/pdf","file_size":7925886,"creator":"dernst"}]},{"issue":"6","abstract":[{"text":"Dynamic reorganization of the cytoplasm is key to many core cellular processes, such as cell division, cell migration, and cell polarization. Cytoskeletal rearrangements are thought to constitute the main drivers of cytoplasmic flows and reorganization. In contrast, remarkably little is known about how dynamic changes in size and shape of cell organelles affect cytoplasmic organization. Here, we show that within the maturing zebrafish oocyte, the surface localization of exocytosis-competent cortical granules (Cgs) upon germinal vesicle breakdown (GVBD) is achieved by the combined activities of yolk granule (Yg) fusion and microtubule aster formation and translocation. We find that Cgs are moved towards the oocyte surface through radially outward cytoplasmic flows induced by Ygs fusing and compacting towards the oocyte center in response to GVBD. We further show that vesicles decorated with the small Rab GTPase Rab11, a master regulator of vesicular trafficking and exocytosis, accumulate together with Cgs at the oocyte surface. This accumulation is achieved by Rab11-positive vesicles being transported by acentrosomal microtubule asters, the formation of which is induced by the release of CyclinB/Cdk1 upon GVBD, and which display a net movement towards the oocyte surface by preferentially binding to the oocyte actin cortex. We finally demonstrate that the decoration of Cgs by Rab11 at the oocyte surface is needed for Cg exocytosis and subsequent chorion elevation, a process central in egg activation. Collectively, these findings unravel a yet unrecognized role of organelle fusion, functioning together with cytoskeletal rearrangements, in orchestrating cytoplasmic organization during oocyte maturation.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","file":[{"access_level":"open_access","file_name":"2023_PloSBiology_Shamipour.pdf","content_type":"application/pdf","file_size":4431723,"creator":"dernst","relation":"main_file","file_id":"13246","checksum":"8e88cb0e5a6433a2f1939a9030bed384","success":1,"date_updated":"2023-07-18T07:59:58Z","date_created":"2023-07-18T07:59:58Z"}],"_id":"13229","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":" 21","title":"Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes","status":"public","ddc":["570"],"has_accepted_license":"1","article_processing_charge":"No","day":"08","scopus_import":"1","date_published":"2023-06-08T00:00:00Z","citation":{"ama":"Shamipour S, Hofmann L, Steccari I, Kardos R, Heisenberg C-PJ. Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes. PLoS Biology. 2023;21(6):e3002146. doi:10.1371/journal.pbio.3002146","ista":"Shamipour S, Hofmann L, Steccari I, Kardos R, Heisenberg C-PJ. 2023. Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes. PLoS Biology. 21(6), e3002146.","ieee":"S. Shamipour, L. Hofmann, I. Steccari, R. Kardos, and C.-P. J. Heisenberg, “Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes,” PLoS Biology, vol. 21, no. 6. Public Library of Science, p. e3002146, 2023.","apa":"Shamipour, S., Hofmann, L., Steccari, I., Kardos, R., & Heisenberg, C.-P. J. (2023). Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes. PLoS Biology. Public Library of Science. https://doi.org/10.1371/journal.pbio.3002146","mla":"Shamipour, Shayan, et al. “Yolk Granule Fusion and Microtubule Aster Formation Regulate Cortical Granule Translocation and Exocytosis in Zebrafish Oocytes.” PLoS Biology, vol. 21, no. 6, Public Library of Science, 2023, p. e3002146, doi:10.1371/journal.pbio.3002146.","short":"S. Shamipour, L. Hofmann, I. Steccari, R. Kardos, C.-P.J. Heisenberg, PLoS Biology 21 (2023) e3002146.","chicago":"Shamipour, Shayan, Laura Hofmann, Irene Steccari, Roland Kardos, and Carl-Philipp J Heisenberg. “Yolk Granule Fusion and Microtubule Aster Formation Regulate Cortical Granule Translocation and Exocytosis in Zebrafish Oocytes.” PLoS Biology. Public Library of Science, 2023. https://doi.org/10.1371/journal.pbio.3002146."},"publication":"PLoS Biology","page":"e3002146","article_type":"original","ec_funded":1,"file_date_updated":"2023-07-18T07:59:58Z","author":[{"last_name":"Shamipour","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan"},{"full_name":"Hofmann, Laura","first_name":"Laura","last_name":"Hofmann","id":"b88d43f2-dc74-11ea-a0a7-e41b7912e031"},{"id":"2705C766-9FE2-11EA-B224-C6773DDC885E","first_name":"Irene","last_name":"Steccari","full_name":"Steccari, Irene"},{"id":"4039350E-F248-11E8-B48F-1D18A9856A87","first_name":"Roland","last_name":"Kardos","full_name":"Kardos, Roland"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"volume":21,"date_created":"2023-07-16T22:01:09Z","date_updated":"2023-08-02T06:33:14Z","pmid":1,"acknowledgement":"This work was supported by funding from the European Union (European Research Council Advanced grant 742573) to C.-P.H. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","year":"2023","department":[{"_id":"CaHe"}],"publisher":"Public Library of Science","publication_status":"published","publication_identifier":{"eissn":["1545-7885"]},"month":"06","doi":"10.1371/journal.pbio.3002146","language":[{"iso":"eng"}],"external_id":{"pmid":["37289834"],"isi":["001003199100005"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"}],"quality_controlled":"1","isi":1},{"author":[{"first_name":"Elod","last_name":"Méhes","full_name":"Méhes, Elod"},{"full_name":"Mones, Enys","first_name":"Enys","last_name":"Mones"},{"full_name":"Varga, Máté","first_name":"Máté","last_name":"Varga"},{"full_name":"Zsigmond, Áron","last_name":"Zsigmond","first_name":"Áron"},{"full_name":"Biri-Kovács, Beáta","last_name":"Biri-Kovács","first_name":"Beáta"},{"full_name":"Nyitray, László","first_name":"László","last_name":"Nyitray"},{"full_name":"Barone, Vanessa","first_name":"Vanessa","last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367"},{"full_name":"Krens, Gabriel","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"},{"first_name":"Tamás","last_name":"Vicsek","full_name":"Vicsek, Tamás"}],"date_created":"2023-08-13T22:01:13Z","date_updated":"2023-12-13T12:07:33Z","volume":6,"acknowledgement":"We thank Marton Gulyas (ELTE Eötvös University) for development of videomicroscopy experiment manager and image analysis software. Authors are grateful to Gabor Forgacs (University of Missouri) for critical reading of earlier versions of this manuscript as well as to Zsuzsa Akos and Andras Czirok (ELTE Eötvös University) for fruitful discussions. This work was supported by EU FP7, ERC COLLMOT Project No 227878 to TV, the National Research Development and Innovation Fund of Hungary, K119359 and also Project No 2018-1.2.1-NKP-2018-00005 to LN. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 955576. MV was supported by the Ja´nos Bolyai Fellowship of the Hungarian Academy of Sciences.\r\nOpen access funding provided by Eötvös Loránd University.","year":"2023","pmid":1,"publication_status":"published","publisher":"Springer Nature","department":[{"_id":"CaHe"},{"_id":"Bio"}],"file_date_updated":"2023-08-14T07:17:36Z","article_number":"817","doi":"10.1038/s42003-023-05181-7","language":[{"iso":"eng"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["37542157"],"isi":["001042544100001"]},"isi":1,"quality_controlled":"1","month":"08","publication_identifier":{"eissn":["2399-3642"]},"file":[{"file_name":"2023_CommBiology_Mehes.pdf","access_level":"open_access","creator":"dernst","content_type":"application/pdf","file_size":10181997,"file_id":"14045","relation":"main_file","date_created":"2023-08-14T07:17:36Z","date_updated":"2023-08-14T07:17:36Z","success":1,"checksum":"1f9324f736bdbb76426b07736651c4cd"}],"oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"14041","title":"3D cell segregation geometry and dynamics are governed by tissue surface tension regulation","status":"public","ddc":["570"],"intvolume":" 6","abstract":[{"lang":"eng","text":"Tissue morphogenesis and patterning during development involve the segregation of cell types. Segregation is driven by differential tissue surface tensions generated by cell types through controlling cell-cell contact formation by regulating adhesion and actomyosin contractility-based cellular cortical tensions. We use vertebrate tissue cell types and zebrafish germ layer progenitors as in vitro models of 3-dimensional heterotypic segregation and developed a quantitative analysis of their dynamics based on 3D time-lapse microscopy. We show that general inhibition of actomyosin contractility by the Rho kinase inhibitor Y27632 delays segregation. Cell type-specific inhibition of non-muscle myosin2 activity by overexpression of myosin assembly inhibitor S100A4 reduces tissue surface tension, manifested in decreased compaction during aggregation and inverted geometry observed during segregation. The same is observed when we express a constitutively active Rho kinase isoform to ubiquitously keep actomyosin contractility high at cell-cell and cell-medium interfaces and thus overriding the interface-specific regulation of cortical tensions. Tissue surface tension regulation can become an effective tool in tissue engineering."}],"type":"journal_article","date_published":"2023-08-04T00:00:00Z","publication":"Communications Biology","citation":{"short":"E. Méhes, E. Mones, M. Varga, Á. Zsigmond, B. Biri-Kovács, L. Nyitray, V. Barone, G. Krens, C.-P.J. Heisenberg, T. Vicsek, Communications Biology 6 (2023).","mla":"Méhes, Elod, et al. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue Surface Tension Regulation.” Communications Biology, vol. 6, 817, Springer Nature, 2023, doi:10.1038/s42003-023-05181-7.","chicago":"Méhes, Elod, Enys Mones, Máté Varga, Áron Zsigmond, Beáta Biri-Kovács, László Nyitray, Vanessa Barone, Gabriel Krens, Carl-Philipp J Heisenberg, and Tamás Vicsek. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue Surface Tension Regulation.” Communications Biology. Springer Nature, 2023. https://doi.org/10.1038/s42003-023-05181-7.","ama":"Méhes E, Mones E, Varga M, et al. 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. Communications Biology. 2023;6. doi:10.1038/s42003-023-05181-7","ieee":"E. Méhes et al., “3D cell segregation geometry and dynamics are governed by tissue surface tension regulation,” Communications Biology, vol. 6. Springer Nature, 2023.","apa":"Méhes, E., Mones, E., Varga, M., Zsigmond, Á., Biri-Kovács, B., Nyitray, L., … Vicsek, T. (2023). 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. Communications Biology. Springer Nature. https://doi.org/10.1038/s42003-023-05181-7","ista":"Méhes E, Mones E, Varga M, Zsigmond Á, Biri-Kovács B, Nyitray L, Barone V, Krens G, Heisenberg C-PJ, Vicsek T. 2023. 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. Communications Biology. 6, 817."},"article_type":"original","day":"04","article_processing_charge":"Yes","has_accepted_license":"1","scopus_import":"1"},{"month":"08","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"doi":"10.1242/jcs.260668","quality_controlled":"1","isi":1,"project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"}],"external_id":{"isi":["001070149000001"]},"file_date_updated":"2023-08-21T07:37:54Z","ec_funded":1,"article_number":"jcs260668","date_created":"2023-08-20T22:01:13Z","date_updated":"2023-12-13T12:11:18Z","volume":136,"author":[{"full_name":"Higashi, Tomohito","last_name":"Higashi","first_name":"Tomohito"},{"full_name":"Stephenson, Rachel E.","first_name":"Rachel E.","last_name":"Stephenson"},{"full_name":"Schwayer, Cornelia","first_name":"Cornelia","last_name":"Schwayer","id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226"},{"full_name":"Huljev, Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","last_name":"Huljev","first_name":"Karla"},{"first_name":"Atsuko Y.","last_name":"Higashi","full_name":"Higashi, Atsuko Y."},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Chiba, Hideki","first_name":"Hideki","last_name":"Chiba"},{"last_name":"Miller","first_name":"Ann L.","full_name":"Miller, Ann L."}],"publication_status":"published","publisher":"The Company of Biologists","department":[{"_id":"CaHe"},{"_id":"EvBe"}],"acknowledgement":"The authors thank their respective lab members for feedback and helpful discussions. We thank the bioimaging and zebrafish facilities of IST Austria for their support.\r\nThis work was supported by the National Institutes of Health [R01GM112794 to A.L.M.], by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science [21K06156 to T.H.], by the Grant Program for Biomedical Engineering Research from the Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering [to T.H.] and by funding from the European Research Council [advanced grant 742573 to C.-P.H.]. ","year":"2023","day":"01","article_processing_charge":"No","has_accepted_license":"1","scopus_import":"1","date_published":"2023-08-01T00:00:00Z","article_type":"original","publication":"Journal of Cell Science","citation":{"ista":"Higashi T, Stephenson RE, Schwayer C, Huljev K, Higashi AY, Heisenberg C-PJ, Chiba H, Miller AL. 2023. ZnUMBA - a live imaging method to detect local barrier breaches. Journal of Cell Science. 136(15), jcs260668.","ieee":"T. Higashi et al., “ZnUMBA - a live imaging method to detect local barrier breaches,” Journal of Cell Science, vol. 136, no. 15. The Company of Biologists, 2023.","apa":"Higashi, T., Stephenson, R. E., Schwayer, C., Huljev, K., Higashi, A. Y., Heisenberg, C.-P. J., … Miller, A. L. (2023). ZnUMBA - a live imaging method to detect local barrier breaches. Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.260668","ama":"Higashi T, Stephenson RE, Schwayer C, et al. ZnUMBA - a live imaging method to detect local barrier breaches. Journal of Cell Science. 2023;136(15). doi:10.1242/jcs.260668","chicago":"Higashi, Tomohito, Rachel E. Stephenson, Cornelia Schwayer, Karla Huljev, Atsuko Y. Higashi, Carl-Philipp J Heisenberg, Hideki Chiba, and Ann L. Miller. “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” Journal of Cell Science. The Company of Biologists, 2023. https://doi.org/10.1242/jcs.260668.","mla":"Higashi, Tomohito, et al. “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” Journal of Cell Science, vol. 136, no. 15, jcs260668, The Company of Biologists, 2023, doi:10.1242/jcs.260668.","short":"T. Higashi, R.E. Stephenson, C. Schwayer, K. Huljev, A.Y. Higashi, C.-P.J. Heisenberg, H. Chiba, A.L. Miller, Journal of Cell Science 136 (2023)."},"abstract":[{"lang":"eng","text":"Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods measure changes in global barrier function, they lack the sensitivity needed to detect local or transient barrier breaches, and they do not reveal the location of barrier leaks. Therefore, we previously developed a method that we named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which overcomes these limitations, allowing for detection of local tight junction leaks with high spatiotemporal resolution. Here, we present expanded applications for ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier restoration and actin accumulation following laser injury. ZnUMBA can also be effectively utilized in developing zebrafish embryos as well as cultured monolayers of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful and flexible method that, with minimal optimization, can be applied to multiple systems to measure dynamic changes in barrier function with spatiotemporal precision."}],"issue":"15","type":"journal_article","file":[{"embargo":"2024-08-10","file_id":"14092","relation":"main_file","checksum":"a399389b7e3d072f1788b63e612a10b3","date_created":"2023-08-21T07:37:54Z","date_updated":"2023-08-21T07:37:54Z","access_level":"closed","file_name":"2023_JourCellScience_Higashi.pdf","embargo_to":"open_access","creator":"dernst","content_type":"application/pdf","file_size":18665315}],"oa_version":"None","title":"ZnUMBA - a live imaging method to detect local barrier breaches","status":"public","ddc":["570"],"intvolume":" 136","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"14082"},{"year":"2022","acknowledgement":"This research was supported by the Scientific Service Units of IST Austria through resources provided by the Imaging and Optics, Electron Microscopy, Preclinical and Life Science Facilities. We thank C. Moussion for providing anti-PNAd antibody and D. Critchley for Talin1-floxed mice, and E. Papusheva for providing a custom 3D channel alignment script. This work was supported by a European Research Council grant ERC-CoG-72437 to M.S. M.H. was supported by Czech Sciencundation GACR 20-24603Y and Charles University PRIMUS/20/MED/013.","publisher":"Springer Nature","department":[{"_id":"SiHi"},{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"MiSi"}],"publication_status":"published","author":[{"full_name":"Assen, Frank P","first_name":"Frank P","last_name":"Assen","id":"3A8E7F24-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3470-6119"},{"last_name":"Abe","first_name":"Jun","full_name":"Abe, Jun"},{"full_name":"Hons, Miroslav","id":"4167FE56-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6625-3348","first_name":"Miroslav","last_name":"Hons"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert"},{"last_name":"Shamipour","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan"},{"first_name":"Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter"},{"first_name":"Tommaso","last_name":"Costanzo","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","orcid":"0000-0001-9732-3815","full_name":"Costanzo, Tommaso"},{"id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996","first_name":"Gabriel","last_name":"Krens","full_name":"Krens, Gabriel"},{"full_name":"Brown, Markus","first_name":"Markus","last_name":"Brown","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ludewig, Burkhard","first_name":"Burkhard","last_name":"Ludewig"},{"first_name":"Simon","last_name":"Hippenmeyer","id":"37B36620-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2279-1061","full_name":"Hippenmeyer, Simon"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Weninger, Wolfgang","last_name":"Weninger","first_name":"Wolfgang"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B"},{"full_name":"Luther, Sanjiv A.","first_name":"Sanjiv A.","last_name":"Luther"},{"last_name":"Stein","first_name":"Jens V.","full_name":"Stein, Jens V."},{"full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K","orcid":"0000-0002-4561-241X","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"volume":23,"date_created":"2021-08-06T09:09:11Z","date_updated":"2023-08-02T06:53:07Z","ec_funded":1,"file_date_updated":"2022-07-25T07:11:32Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"isi":["000822975900002"]},"project":[{"call_identifier":"H2020","name":"Cellular navigation along spatial gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373"}],"isi":1,"quality_controlled":"1","doi":"10.1038/s41590-022-01257-4","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"PreCl"},{"_id":"LifeSc"}],"publication_identifier":{"eissn":["1529-2916"],"issn":["1529-2908"]},"month":"07","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9794","intvolume":" 23","title":"Multitier mechanics control stromal adaptations in swelling lymph nodes","status":"public","ddc":["570"],"file":[{"checksum":"628e7b49809f22c75b428842efe70c68","success":1,"date_updated":"2022-07-25T07:11:32Z","date_created":"2022-07-25T07:11:32Z","relation":"main_file","file_id":"11642","file_size":11475325,"content_type":"application/pdf","creator":"dernst","access_level":"open_access","file_name":"2022_NatureImmunology_Assen.pdf"}],"oa_version":"Published Version","type":"journal_article","abstract":[{"text":"Lymph nodes (LNs) comprise two main structural elements: fibroblastic reticular cells that form dedicated niches for immune cell interaction and capsular fibroblasts that build a shell around the organ. Immunological challenge causes LNs to increase more than tenfold in size within a few days. Here, we characterized the biomechanics of LN swelling on the cellular and organ scale. We identified lymphocyte trapping by influx and proliferation as drivers of an outward pressure force, causing fibroblastic reticular cells of the T-zone (TRCs) and their associated conduits to stretch. After an initial phase of relaxation, TRCs sensed the resulting strain through cell matrix adhesions, which coordinated local growth and remodeling of the stromal network. While the expanded TRC network readopted its typical configuration, a massive fibrotic reaction of the organ capsule set in and countered further organ expansion. Thus, different fibroblast populations mechanically control LN swelling in a multitier fashion.","lang":"eng"}],"citation":{"ama":"Assen FP, Abe J, Hons M, et al. Multitier mechanics control stromal adaptations in swelling lymph nodes. Nature Immunology. 2022;23:1246-1255. doi:10.1038/s41590-022-01257-4","apa":"Assen, F. P., Abe, J., Hons, M., Hauschild, R., Shamipour, S., Kaufmann, W., … Sixt, M. K. (2022). Multitier mechanics control stromal adaptations in swelling lymph nodes. Nature Immunology. Springer Nature. https://doi.org/10.1038/s41590-022-01257-4","ieee":"F. P. Assen et al., “Multitier mechanics control stromal adaptations in swelling lymph nodes,” Nature Immunology, vol. 23. Springer Nature, pp. 1246–1255, 2022.","ista":"Assen FP, Abe J, Hons M, Hauschild R, Shamipour S, Kaufmann W, Costanzo T, Krens G, Brown M, Ludewig B, Hippenmeyer S, Heisenberg C-PJ, Weninger W, Hannezo EB, Luther SA, Stein JV, Sixt MK. 2022. Multitier mechanics control stromal adaptations in swelling lymph nodes. Nature Immunology. 23, 1246–1255.","short":"F.P. Assen, J. Abe, M. Hons, R. Hauschild, S. Shamipour, W. Kaufmann, T. Costanzo, G. Krens, M. Brown, B. Ludewig, S. Hippenmeyer, C.-P.J. Heisenberg, W. Weninger, E.B. Hannezo, S.A. Luther, J.V. Stein, M.K. Sixt, Nature Immunology 23 (2022) 1246–1255.","mla":"Assen, Frank P., et al. “Multitier Mechanics Control Stromal Adaptations in Swelling Lymph Nodes.” Nature Immunology, vol. 23, Springer Nature, 2022, pp. 1246–55, doi:10.1038/s41590-022-01257-4.","chicago":"Assen, Frank P, Jun Abe, Miroslav Hons, Robert Hauschild, Shayan Shamipour, Walter Kaufmann, Tommaso Costanzo, et al. “Multitier Mechanics Control Stromal Adaptations in Swelling Lymph Nodes.” Nature Immunology. Springer Nature, 2022. https://doi.org/10.1038/s41590-022-01257-4."},"publication":"Nature Immunology","page":"1246-1255","article_type":"original","date_published":"2022-07-11T00:00:00Z","scopus_import":"1","has_accepted_license":"1","article_processing_charge":"No","day":"11"},{"publisher":"Cell Press","department":[{"_id":"EdHa"},{"_id":"CaHe"}],"publication_status":"published","pmid":1,"year":"2022","acknowledgement":"We thank present and former members of the Heisenberg and Hannezo groups, in particular Bernat Corominas-Murtra and Nicoletta Petridou, for helpful discussions, and Claudia Flandoli for the artwork. We apologize for not being able to cite a number of highly relevant studies, to stay within the maximum allowed number of citations.","volume":32,"date_updated":"2023-08-02T14:03:53Z","date_created":"2022-01-30T23:01:34Z","author":[{"last_name":"Hannezo","first_name":"Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"isi":1,"quality_controlled":"1","external_id":{"pmid":["35058104"],"isi":["000795773900009"]},"language":[{"iso":"eng"}],"doi":"10.1016/j.tcb.2021.12.006","publication_identifier":{"eissn":["1879-3088"],"issn":["0962-8924"]},"month":"05","intvolume":" 32","title":"Rigidity transitions in development and disease","status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"10705","oa_version":"None","type":"journal_article","issue":"5","abstract":[{"text":"Although rigidity and jamming transitions have been widely studied in physics and material science, their importance in a number of biological processes, including embryo development, tissue homeostasis, wound healing, and disease progression, has only begun to be recognized in the past few years. The hypothesis that biological systems can undergo rigidity/jamming transitions is attractive, as it would allow these systems to change their material properties rapidly and strongly. However, whether such transitions indeed occur in biological systems, how they are being regulated, and what their physiological relevance might be, is still being debated. Here, we review theoretical and experimental advances from the past few years, focusing on the regulation and role of potential tissue rigidity transitions in different biological processes.","lang":"eng"}],"page":"P433-444","article_type":"original","citation":{"chicago":"Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Rigidity Transitions in Development and Disease.” Trends in Cell Biology. Cell Press, 2022. https://doi.org/10.1016/j.tcb.2021.12.006.","mla":"Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Rigidity Transitions in Development and Disease.” Trends in Cell Biology, vol. 32, no. 5, Cell Press, 2022, pp. P433-444, doi:10.1016/j.tcb.2021.12.006.","short":"E.B. Hannezo, C.-P.J. Heisenberg, Trends in Cell Biology 32 (2022) P433-444.","ista":"Hannezo EB, Heisenberg C-PJ. 2022. Rigidity transitions in development and disease. Trends in Cell Biology. 32(5), P433-444.","ieee":"E. B. Hannezo and C.-P. J. Heisenberg, “Rigidity transitions in development and disease,” Trends in Cell Biology, vol. 32, no. 5. Cell Press, pp. P433-444, 2022.","apa":"Hannezo, E. B., & Heisenberg, C.-P. J. (2022). Rigidity transitions in development and disease. Trends in Cell Biology. Cell Press. https://doi.org/10.1016/j.tcb.2021.12.006","ama":"Hannezo EB, Heisenberg C-PJ. Rigidity transitions in development and disease. Trends in Cell Biology. 2022;32(5):P433-444. doi:10.1016/j.tcb.2021.12.006"},"publication":"Trends in Cell Biology","date_published":"2022-05-01T00:00:00Z","scopus_import":"1","article_processing_charge":"No","day":"01"},{"publication_identifier":{"eissn":["10916490"]},"month":"02","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"PreCl"}],"doi":"10.1073/pnas.2122030119","project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"},{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"},{"_id":"2521E28E-B435-11E9-9278-68D0E5697425","grant_number":"187-2013","name":"Modulation of adhesion function in cell-cell contact formation by cortical tension"}],"isi":1,"quality_controlled":"1","external_id":{"isi":["000766926900009"]},"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","ec_funded":1,"file_date_updated":"2022-02-21T08:45:11Z","article_number":"e2122030119","volume":119,"date_updated":"2023-08-02T14:26:51Z","date_created":"2022-02-20T23:01:31Z","related_material":{"record":[{"id":"9750","status":"public","relation":"earlier_version"}]},"author":[{"full_name":"Slovakova, Jana","id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87","last_name":"Slovakova","first_name":"Jana"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","last_name":"Sikora","first_name":"Mateusz K","full_name":"Sikora, Mateusz K"},{"full_name":"Arslan, Feyza N","last_name":"Arslan","first_name":"Feyza N","orcid":"0000-0001-5809-9566","id":"49DA7910-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Caballero Mancebo, Silvia","first_name":"Silvia","last_name":"Caballero Mancebo","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5223-3346"},{"full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996","first_name":"Gabriel","last_name":"Krens"},{"last_name":"Kaufmann","first_name":"Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","full_name":"Kaufmann, Walter"},{"full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","first_name":"Jack","last_name":"Merrin"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"department":[{"_id":"CaHe"},{"_id":"EM-Fac"},{"_id":"Bio"}],"publisher":"Proceedings of the National Academy of Sciences","publication_status":"published","year":"2022","acknowledgement":"We thank Guillaume Salbreaux, Silvia Grigolon, Edouard Hannezo, and Vanessa Barone for discussions and comments on the manuscript and Shayan Shamipour and Daniel Capek for help with data analysis. We also thank the Imaging & Optics, Electron Microscopy, and Zebrafish Facility Scientific Service Units at the Institute of Science and Technology Austria (ISTA)Nasser Darwish-Miranda for continuous support. We acknowledge Hitoshi Morita for the gift of VinculinB-GFP plasmid. This research was supported by an ISTA Fellow Marie-Curie Co-funding of regional, national, and international programmes Grant P_IST_EU01 (to J.S.), European Molecular Biology Organization Long-Term Fellowship Grant, ALTF reference number: 187-2013 (to M.S.), Schroedinger Fellowship J4332-B28 (to M.S.), and European Research Council Advanced Grant (MECSPEC; to C.-P.H.).","has_accepted_license":"1","article_processing_charge":"No","day":"14","scopus_import":"1","date_published":"2022-02-14T00:00:00Z","article_type":"original","citation":{"apa":"Slovakova, J., Sikora, M. K., Arslan, F. N., Caballero Mancebo, S., Krens, G., Kaufmann, W., … Heisenberg, C.-P. J. (2022). Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells. Proceedings of the National Academy of Sciences of the United States of America. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.2122030119","ieee":"J. Slovakova et al., “Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 8. Proceedings of the National Academy of Sciences, 2022.","ista":"Slovakova J, Sikora MK, Arslan FN, Caballero Mancebo S, Krens G, Kaufmann W, Merrin J, Heisenberg C-PJ. 2022. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells. Proceedings of the National Academy of Sciences of the United States of America. 119(8), e2122030119.","ama":"Slovakova J, Sikora MK, Arslan FN, et al. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells. Proceedings of the National Academy of Sciences of the United States of America. 2022;119(8). doi:10.1073/pnas.2122030119","chicago":"Slovakova, Jana, Mateusz K Sikora, Feyza N Arslan, Silvia Caballero Mancebo, Gabriel Krens, Walter Kaufmann, Jack Merrin, and Carl-Philipp J Heisenberg. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion in Zebrafish Germ-Layer Progenitor Cells.” Proceedings of the National Academy of Sciences of the United States of America. Proceedings of the National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2122030119.","short":"J. Slovakova, M.K. Sikora, F.N. Arslan, S. Caballero Mancebo, G. Krens, W. Kaufmann, J. Merrin, C.-P.J. Heisenberg, Proceedings of the National Academy of Sciences of the United States of America 119 (2022).","mla":"Slovakova, Jana, et al. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion in Zebrafish Germ-Layer Progenitor Cells.” Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 8, e2122030119, Proceedings of the National Academy of Sciences, 2022, doi:10.1073/pnas.2122030119."},"publication":"Proceedings of the National Academy of Sciences of the United States of America","issue":"8","abstract":[{"lang":"eng","text":"Tension of the actomyosin cell cortex plays a key role in determining cell–cell contact growth and size. The level of cortical tension outside of the cell–cell contact, when pulling at the contact edge, scales with the total size to which a cell–cell contact can grow [J.-L. Maître et al., Science 338, 253–256 (2012)]. Here, we show in zebrafish primary germ-layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell–cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. After tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell–cell contact size is limited by tension-stabilizing E-cadherin–actin complexes at the contact."}],"type":"journal_article","oa_version":"Published Version","file":[{"creator":"dernst","content_type":"application/pdf","file_size":1609678,"access_level":"open_access","file_name":"2022_PNAS_Slovakova.pdf","success":1,"checksum":"d49f83c3580613966f71768ddb9a55a5","date_created":"2022-02-21T08:45:11Z","date_updated":"2022-02-21T08:45:11Z","file_id":"10780","relation":"main_file"}],"intvolume":" 119","title":"Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells","ddc":["570"],"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"10766"},{"quality_controlled":"1","isi":1,"project":[{"name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation","grant_number":"ALTF 850-2017","_id":"26520D1E-B435-11E9-9278-68D0E5697425"},{"name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation","grant_number":"ALTF 850-2017","_id":"26520D1E-B435-11E9-9278-68D0E5697425"},{"call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288"},{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000871319900002"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"language":[{"iso":"eng"}],"doi":"10.1038/s41567-022-01787-6","month":"12","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"publication_status":"published","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"publisher":"Springer Nature","year":"2022","acknowledgement":"We thank K. Sampath, A. Pauli and Y. Bellaїche for feedback on the manuscript. We also thank the members of the Heisenberg group, in particular A. Schauer and F. Nur Arslan, for help, technical advice and discussions, and the Bioimaging and Life Science facilities at IST\r\nAustria for continuous support. We thank C. Flandoli for the artwork in the figures. This work was supported by postdoctoral fellowships from EMBO (LTF-850-2017) and HFSP (LT000429/2018-L2) to D.P. and the European Union (European Research Council starting grant 851288 to É.H. and European Research Council advanced grant 742573 to C.-P.H.).","date_created":"2023-01-16T09:45:19Z","date_updated":"2023-08-04T09:15:58Z","volume":18,"author":[{"full_name":"Nunes Pinheiro, Diana C","last_name":"Nunes Pinheiro","first_name":"Diana C","orcid":"0000-0003-4333-7503","id":"2E839F16-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kardos, Roland","first_name":"Roland","last_name":"Kardos","id":"4039350E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hannezo, Edouard B","first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"file_date_updated":"2023-01-27T07:32:01Z","ec_funded":1,"article_type":"original","page":"1482-1493","publication":"Nature Physics","citation":{"apa":"Nunes Pinheiro, D. C., Kardos, R., Hannezo, E. B., & Heisenberg, C.-P. J. (2022). Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-022-01787-6","ieee":"D. C. Nunes Pinheiro, R. Kardos, E. B. Hannezo, and C.-P. J. Heisenberg, “Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming,” Nature Physics, vol. 18, no. 12. Springer Nature, pp. 1482–1493, 2022.","ista":"Nunes Pinheiro DC, Kardos R, Hannezo EB, Heisenberg C-PJ. 2022. Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming. Nature Physics. 18(12), 1482–1493.","ama":"Nunes Pinheiro DC, Kardos R, Hannezo EB, Heisenberg C-PJ. Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming. Nature Physics. 2022;18(12):1482-1493. doi:10.1038/s41567-022-01787-6","chicago":"Nunes Pinheiro, Diana C, Roland Kardos, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Morphogen Gradient Orchestrates Pattern-Preserving Tissue Morphogenesis via Motility-Driven Unjamming.” Nature Physics. Springer Nature, 2022. https://doi.org/10.1038/s41567-022-01787-6.","short":"D.C. Nunes Pinheiro, R. Kardos, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 18 (2022) 1482–1493.","mla":"Nunes Pinheiro, Diana C., et al. “Morphogen Gradient Orchestrates Pattern-Preserving Tissue Morphogenesis via Motility-Driven Unjamming.” Nature Physics, vol. 18, no. 12, Springer Nature, 2022, pp. 1482–93, doi:10.1038/s41567-022-01787-6."},"date_published":"2022-12-01T00:00:00Z","keyword":["General Physics and Astronomy"],"scopus_import":"1","day":"01","has_accepted_license":"1","article_processing_charge":"No","status":"public","title":"Morphogen gradient orchestrates pattern-preserving tissue morphogenesis via motility-driven unjamming","ddc":["570"],"intvolume":" 18","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"12209","oa_version":"Published Version","file":[{"access_level":"open_access","file_name":"2022_NaturePhysics_Pinheiro.pdf","creator":"dernst","file_size":36703569,"content_type":"application/pdf","file_id":"12412","relation":"main_file","success":1,"checksum":"c86a8e8d80d1bfc46d56a01e88a2526a","date_created":"2023-01-27T07:32:01Z","date_updated":"2023-01-27T07:32:01Z"}],"type":"journal_article","abstract":[{"text":"Embryo development requires biochemical signalling to generate patterns of cell fates and active mechanical forces to drive tissue shape changes. However, how these processes are coordinated, and how tissue patterning is preserved despite the cellular flows occurring during morphogenesis, remains poorly understood. Gastrulation is a crucial embryonic stage that involves both patterning and internalization of the mesendoderm germ layer tissue. Here we show that, in zebrafish embryos, a gradient in Nodal signalling orchestrates pattern-preserving internalization movements by triggering a motility-driven unjamming transition. In addition to its role as a morphogen determining embryo patterning, graded Nodal signalling mechanically subdivides the mesendoderm into a small fraction of highly protrusive leader cells, able to autonomously internalize via local unjamming, and less protrusive followers, which need to be pulled inwards by the leaders. The Nodal gradient further enforces a code of preferential adhesion coupling leaders to their immediate followers, resulting in a collective and ordered mode of internalization that preserves mesendoderm patterning. Integrating this dual mechanical role of Nodal signalling into minimal active particle simulations quantitatively predicts both physiological and experimentally perturbed internalization movements. This provides a quantitative framework for how a morphogen-encoded unjamming transition can bidirectionally couple tissue mechanics with patterning during complex three-dimensional morphogenesis.","lang":"eng"}],"issue":"12"},{"article_number":"dev200215","file_date_updated":"2023-01-27T10:36:50Z","pmid":1,"year":"2022","acknowledgement":"iona intestinalis adults were provided by Dr Yutaka Satou (Kyoto University) and Dr Manabu Yoshida (the University of Tokyo) with support from the National Bio-Resource Project of AMED, Japan. We thank Dr Hidehiko Hashimoto and Dr Yuji Mizotani for technical information about 1P-myosin antibody staining. We thank Dr Kaoru Imai and Dr Yutaka Satou for valuable discussion about Admp and for the DNA construct of Bmp2/4 under the Dlx.b upstream sequence. We thank Ms Maki Kogure for constructing the FUSION360 of the intercalating epidermal cell.\r\nThis work was supported by funding from the Japan Society for the Promotion of Science (JP16H01451, JP21H00440). Open Access funding provided by Keio University: Keio Gijuku Daigaku.","department":[{"_id":"CaHe"}],"publisher":"The Company of Biologists","publication_status":"published","author":[{"last_name":"Kogure","first_name":"Yuki S.","full_name":"Kogure, Yuki S."},{"last_name":"Muraoka","first_name":"Hiromochi","full_name":"Muraoka, Hiromochi"},{"first_name":"Wataru C.","last_name":"Koizumi","full_name":"Koizumi, Wataru C."},{"last_name":"Gelin-alessi","first_name":"Raphaël","full_name":"Gelin-alessi, Raphaël"},{"full_name":"Godard, Benoit G","id":"3263621A-F248-11E8-B48F-1D18A9856A87","first_name":"Benoit G","last_name":"Godard"},{"first_name":"Kotaro","last_name":"Oka","full_name":"Oka, Kotaro"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"last_name":"Hotta","first_name":"Kohji","full_name":"Hotta, Kohji"}],"volume":149,"date_updated":"2023-08-04T09:33:24Z","date_created":"2023-01-16T09:50:12Z","publication_identifier":{"eissn":["1477-9129"],"issn":["0950-1991"]},"month":"11","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["36227591"],"isi":["000903991700002"]},"quality_controlled":"1","isi":1,"doi":"10.1242/dev.200215","language":[{"iso":"eng"}],"type":"journal_article","issue":"21","abstract":[{"lang":"eng","text":"Ventral tail bending, which is transient but pronounced, is found in many chordate embryos and constitutes an interesting model of how tissue interactions control embryo shape. Here, we identify one key upstream regulator of ventral tail bending in embryos of the ascidian Ciona. We show that during the early tailbud stages, ventral epidermal cells exhibit a boat-shaped morphology (boat cell) with a narrow apical surface where phosphorylated myosin light chain (pMLC) accumulates. We further show that interfering with the function of the BMP ligand Admp led to pMLC localizing to the basal instead of the apical side of ventral epidermal cells and a reduced number of boat cells. Finally, we show that cutting ventral epidermal midline cells at their apex using an ultraviolet laser relaxed ventral tail bending. Based on these results, we propose a previously unreported function for Admp in localizing pMLC to the apical side of ventral epidermal cells, which causes the tail to bend ventrally by resisting antero-posterior notochord extension at the ventral side of the tail."}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"12231","intvolume":" 149","title":"Admp regulates tail bending by controlling ventral epidermal cell polarity via phosphorylated myosin localization in Ciona","status":"public","ddc":["570"],"file":[{"success":1,"checksum":"871b9c58eb79b9e60752de25a46938d6","date_created":"2023-01-27T10:36:50Z","date_updated":"2023-01-27T10:36:50Z","file_id":"12423","relation":"main_file","creator":"dernst","file_size":9160451,"content_type":"application/pdf","access_level":"open_access","file_name":"2022_Development_Kogure.pdf"}],"oa_version":"Published Version","scopus_import":"1","keyword":["Developmental Biology","Molecular Biology"],"has_accepted_license":"1","article_processing_charge":"No","day":"01","citation":{"ista":"Kogure YS, Muraoka H, Koizumi WC, Gelin-alessi R, Godard BG, Oka K, Heisenberg C-PJ, Hotta K. 2022. Admp regulates tail bending by controlling ventral epidermal cell polarity via phosphorylated myosin localization in Ciona. Development. 149(21), dev200215.","ieee":"Y. S. Kogure et al., “Admp regulates tail bending by controlling ventral epidermal cell polarity via phosphorylated myosin localization in Ciona,” Development, vol. 149, no. 21. The Company of Biologists, 2022.","apa":"Kogure, Y. S., Muraoka, H., Koizumi, W. C., Gelin-alessi, R., Godard, B. G., Oka, K., … Hotta, K. (2022). Admp regulates tail bending by controlling ventral epidermal cell polarity via phosphorylated myosin localization in Ciona. Development. The Company of Biologists. https://doi.org/10.1242/dev.200215","ama":"Kogure YS, Muraoka H, Koizumi WC, et al. Admp regulates tail bending by controlling ventral epidermal cell polarity via phosphorylated myosin localization in Ciona. Development. 2022;149(21). doi:10.1242/dev.200215","chicago":"Kogure, Yuki S., Hiromochi Muraoka, Wataru C. Koizumi, Raphaël Gelin-alessi, Benoit G Godard, Kotaro Oka, Carl-Philipp J Heisenberg, and Kohji Hotta. “Admp Regulates Tail Bending by Controlling Ventral Epidermal Cell Polarity via Phosphorylated Myosin Localization in Ciona.” Development. The Company of Biologists, 2022. https://doi.org/10.1242/dev.200215.","mla":"Kogure, Yuki S., et al. “Admp Regulates Tail Bending by Controlling Ventral Epidermal Cell Polarity via Phosphorylated Myosin Localization in Ciona.” Development, vol. 149, no. 21, dev200215, The Company of Biologists, 2022, doi:10.1242/dev.200215.","short":"Y.S. Kogure, H. Muraoka, W.C. Koizumi, R. Gelin-alessi, B.G. Godard, K. Oka, C.-P.J. Heisenberg, K. Hotta, Development 149 (2022)."},"publication":"Development","article_type":"original","date_published":"2022-11-01T00:00:00Z"},{"_id":"9245","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Quantifying tissue tension in the granulosa layer after laser surgery","status":"public","intvolume":" 2218","oa_version":"None","type":"book_chapter","alternative_title":["Methods in Molecular Biology"],"abstract":[{"lang":"eng","text":"Tissue morphogenesis is driven by mechanical forces triggering cell movements and shape changes. Quantitatively measuring tension within tissues is of great importance for understanding the role of mechanical signals acting on the cell and tissue level during morphogenesis. Here we introduce laser ablation as a useful tool to probe tissue tension within the granulosa layer, an epithelial monolayer of somatic cells that surround the zebrafish female gamete during folliculogenesis. We describe in detail how to isolate follicles, mount samples, perform laser surgery, and analyze the data."}],"publication":"Germline Development in the Zebrafish","citation":{"chicago":"Xia, Peng, and Carl-Philipp J Heisenberg. “Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery.” In Germline Development in the Zebrafish, edited by Roland Dosch, 2218:117–28. Humana, 2021. https://doi.org/10.1007/978-1-0716-0970-5_10.","short":"P. Xia, C.-P.J. Heisenberg, in:, R. Dosch (Ed.), Germline Development in the Zebrafish, Humana, 2021, pp. 117–128.","mla":"Xia, Peng, and Carl-Philipp J. Heisenberg. “Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery.” Germline Development in the Zebrafish, edited by Roland Dosch, vol. 2218, Humana, 2021, pp. 117–28, doi:10.1007/978-1-0716-0970-5_10.","ieee":"P. Xia and C.-P. J. Heisenberg, “Quantifying tissue tension in the granulosa layer after laser surgery,” in Germline Development in the Zebrafish, vol. 2218, R. Dosch, Ed. Humana, 2021, pp. 117–128.","apa":"Xia, P., & Heisenberg, C.-P. J. (2021). Quantifying tissue tension in the granulosa layer after laser surgery. In R. Dosch (Ed.), Germline Development in the Zebrafish (Vol. 2218, pp. 117–128). Humana. https://doi.org/10.1007/978-1-0716-0970-5_10","ista":"Xia P, Heisenberg C-PJ. 2021.Quantifying tissue tension in the granulosa layer after laser surgery. In: Germline Development in the Zebrafish. Methods in Molecular Biology, vol. 2218, 117–128.","ama":"Xia P, Heisenberg C-PJ. Quantifying tissue tension in the granulosa layer after laser surgery. In: Dosch R, ed. Germline Development in the Zebrafish. Vol 2218. Humana; 2021:117-128. doi:10.1007/978-1-0716-0970-5_10"},"page":"117-128","date_published":"2021-02-20T00:00:00Z","scopus_import":"1","keyword":["Tissue tension","Morphogenesis","Laser ablation","Zebrafish folliculogenesis","Granulosa cells"],"day":"20","article_processing_charge":"No","acknowledgement":"We thank Prof. Masazumi Tada and Roland Dosch for providing transgenic zebrafish lines, the Heisenberg lab for technical assistance and feedback on the manuscript, and the Bioimaging and Fish facilities of IST Austria for continuous support. This work was funded by an ERC advanced grant (MECSPEC to C.-P.H.).","year":"2021","pmid":1,"publication_status":"published","publisher":"Humana","department":[{"_id":"CaHe"}],"editor":[{"full_name":"Dosch, Roland","first_name":"Roland","last_name":"Dosch"}],"author":[{"full_name":"Xia, Peng","orcid":"0000-0002-5419-7756","id":"4AB6C7D0-F248-11E8-B48F-1D18A9856A87","last_name":"Xia","first_name":"Peng"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"date_created":"2021-03-14T23:01:34Z","date_updated":"2022-06-03T10:57:55Z","volume":2218,"ec_funded":1,"external_id":{"pmid":["33606227"]},"quality_controlled":"1","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"}],"doi":"10.1007/978-1-0716-0970-5_10","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"language":[{"iso":"eng"}],"month":"02","publication_identifier":{"eissn":["1940-6029"],"isbn":["978-1-0716-0969-9"],"eisbn":["978-1-0716-0970-5"],"issn":["1064-3745"]}},{"type":"journal_article","abstract":[{"lang":"eng","text":"During development, a single cell is transformed into a highly complex organism through progressive cell division, specification and rearrangement. An important prerequisite for the emergence of patterns within the developing organism is to establish asymmetries at various scales, ranging from individual cells to the entire embryo, eventually giving rise to the different body structures. This becomes especially apparent during gastrulation, when the earliest major lineage restriction events lead to the formation of the different germ layers. Traditionally, the unfolding of the developmental program from symmetry breaking to germ layer formation has been studied by dissecting the contributions of different signaling pathways and cellular rearrangements in the in vivo context of intact embryos. Recent efforts, using the intrinsic capacity of embryonic stem cells to self-assemble and generate embryo-like structures de novo, have opened new avenues for understanding the many ways by which an embryo can be built and the influence of extrinsic factors therein. Here, we discuss and compare divergent and conserved strategies leading to germ layer formation in embryos as compared to in vitro systems, their upstream molecular cascades and the role of extrinsic factors in this process."}],"_id":"8966","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":" 474","ddc":["570"],"status":"public","title":"Reassembling gastrulation","file":[{"date_created":"2021-08-11T10:28:06Z","date_updated":"2021-08-11T10:28:06Z","checksum":"fa2a5731fd16ab171b029f32f031c440","success":1,"relation":"main_file","file_id":"9880","content_type":"application/pdf","file_size":1440321,"creator":"kschuh","file_name":"2021_DevBiology_Schauer.pdf","access_level":"open_access"}],"oa_version":"Published Version","scopus_import":"1","keyword":["Developmental Biology","Cell Biology","Molecular Biology"],"article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","day":"01","citation":{"chicago":"Schauer, Alexandra, and Carl-Philipp J Heisenberg. “Reassembling Gastrulation.” Developmental Biology. Elsevier, 2021. https://doi.org/10.1016/j.ydbio.2020.12.014.","short":"A. Schauer, C.-P.J. Heisenberg, Developmental Biology 474 (2021) 71–81.","mla":"Schauer, Alexandra, and Carl-Philipp J. Heisenberg. “Reassembling Gastrulation.” Developmental Biology, vol. 474, Elsevier, 2021, pp. 71–81, doi:10.1016/j.ydbio.2020.12.014.","ieee":"A. Schauer and C.-P. J. Heisenberg, “Reassembling gastrulation,” Developmental Biology, vol. 474. Elsevier, pp. 71–81, 2021.","apa":"Schauer, A., & Heisenberg, C.-P. J. (2021). Reassembling gastrulation. Developmental Biology. Elsevier. https://doi.org/10.1016/j.ydbio.2020.12.014","ista":"Schauer A, Heisenberg C-PJ. 2021. Reassembling gastrulation. Developmental Biology. 474, 71–81.","ama":"Schauer A, Heisenberg C-PJ. Reassembling gastrulation. Developmental Biology. 2021;474:71-81. doi:10.1016/j.ydbio.2020.12.014"},"publication":"Developmental Biology","page":"71-81","article_type":"original","date_published":"2021-06-01T00:00:00Z","ec_funded":1,"file_date_updated":"2021-08-11T10:28:06Z","acknowledgement":"We thank Nicoletta Petridou, Diana Pinheiro, Cornelia Schwayer and Stefania Tavano for feedback on the manuscript. Research in the Heisenberg lab is supported by an ERC Advanced Grant (MECSPEC 742573) to C.-P.H. A.S. is a recipient of a DOC Fellowship of the Austrian Academy of Science.","year":"2021","department":[{"_id":"CaHe"}],"publisher":"Elsevier","publication_status":"published","related_material":{"record":[{"id":"12891","status":"public","relation":"dissertation_contains"}]},"author":[{"full_name":"Schauer, Alexandra","first_name":"Alexandra","last_name":"Schauer","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7659-9142"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"volume":474,"date_updated":"2023-08-07T13:30:01Z","date_created":"2020-12-22T09:53:34Z","publication_identifier":{"issn":["0012-1606"]},"month":"06","external_id":{"isi":["000639461800008"]},"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"},{"name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues","_id":"26B1E39C-B435-11E9-9278-68D0E5697425","grant_number":"25239"}],"isi":1,"quality_controlled":"1","doi":"10.1016/j.ydbio.2020.12.014","language":[{"iso":"eng"}]},{"date_published":"2021-04-01T00:00:00Z","article_type":"original","page":"1914-1928.e19","publication":"Cell","citation":{"ista":"Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. 2021. Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. Cell. 184(7), 1914–1928.e19.","ieee":"N. Petridou, B. Corominas-Murtra, C.-P. J. Heisenberg, and E. B. Hannezo, “Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions,” Cell, vol. 184, no. 7. Elsevier, p. 1914–1928.e19, 2021.","apa":"Petridou, N., Corominas-Murtra, B., Heisenberg, C.-P. J., & Hannezo, E. B. (2021). Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. Cell. Elsevier. https://doi.org/10.1016/j.cell.2021.02.017","ama":"Petridou N, Corominas-Murtra B, Heisenberg C-PJ, Hannezo EB. Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions. Cell. 2021;184(7):1914-1928.e19. doi:10.1016/j.cell.2021.02.017","chicago":"Petridou, Nicoletta, Bernat Corominas-Murtra, Carl-Philipp J Heisenberg, and Edouard B Hannezo. “Rigidity Percolation Uncovers a Structural Basis for Embryonic Tissue Phase Transitions.” Cell. Elsevier, 2021. https://doi.org/10.1016/j.cell.2021.02.017.","mla":"Petridou, Nicoletta, et al. “Rigidity Percolation Uncovers a Structural Basis for Embryonic Tissue Phase Transitions.” Cell, vol. 184, no. 7, Elsevier, 2021, p. 1914–1928.e19, doi:10.1016/j.cell.2021.02.017.","short":"N. Petridou, B. Corominas-Murtra, C.-P.J. Heisenberg, E.B. Hannezo, Cell 184 (2021) 1914–1928.e19."},"day":"01","has_accepted_license":"1","article_processing_charge":"No","scopus_import":"1","file":[{"date_updated":"2021-06-08T10:04:10Z","date_created":"2021-06-08T10:04:10Z","success":1,"checksum":"1e5295fbd9c2a459173ec45a0e8a7c2e","file_id":"9534","relation":"main_file","creator":"cziletti","content_type":"application/pdf","file_size":11405875,"file_name":"2021_Cell_Petridou.pdf","access_level":"open_access"}],"oa_version":"Published Version","status":"public","title":"Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions","ddc":["570"],"intvolume":" 184","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9316","abstract":[{"lang":"eng","text":"Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural PTs of embryonic tissues from local cell connectivity. By using percolation theory, combined with directly monitoring dynamic changes in tissue rheology and cell contact mechanics, we demonstrate that the zebrafish blastoderm undergoes a genuine rigidity PT, brought about by a small reduction in adhesion-dependent cell connectivity below a critical value. We quantitatively predict and experimentally verify hallmarks of PTs, including power-law exponents and associated discontinuities of macroscopic observables. Finally, we show that this uniform PT depends on blastoderm cells undergoing meta-synchronous divisions causing random and, consequently, uniform changes in cell connectivity. Collectively, our theoretical and experimental findings reveal the structural basis of material PTs in an organismal context."}],"issue":"7","type":"journal_article","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"language":[{"iso":"eng"}],"doi":"10.1016/j.cell.2021.02.017","quality_controlled":"1","isi":1,"project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"},{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis"},{"call_identifier":"FWF","name":"Tissue material properties in embryonic development","grant_number":"V00736","_id":"2693FD8C-B435-11E9-9278-68D0E5697425"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000636734000022"],"pmid":["33730596"]},"month":"04","publication_identifier":{"issn":["00928674"],"eissn":["10974172"]},"date_updated":"2023-08-07T14:33:59Z","date_created":"2021-04-11T22:01:14Z","volume":184,"author":[{"full_name":"Petridou, Nicoletta","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8451-1195","first_name":"Nicoletta","last_name":"Petridou"},{"full_name":"Corominas-Murtra, Bernat","first_name":"Bernat","last_name":"Corominas-Murtra","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9806-5643"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B","full_name":"Hannezo, Edouard B"}],"related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/embryonic-tissue-undergoes-phase-transition/"}]},"publication_status":"published","publisher":"Elsevier","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"acknowledgement":"We thank Carl Goodrich and the members of the Heisenberg and Hannezo groups, in particular Reka Korei, for help, technical advice, and discussions; and the Bioimaging and zebrafish facilities of the IST Austria for continuous support. This work was supported by the Elise Richter Program of Austrian Science Fund (FWF) to N.I.P. ( V 736-B26 ) and the European Union (European Research Council Advanced Grant 742573 to C.-P.H. and European Research Council Starting Grant 851288 to E.H.).","year":"2021","pmid":1,"file_date_updated":"2021-06-08T10:04:10Z","ec_funded":1},{"date_created":"2021-04-25T22:01:30Z","date_updated":"2023-08-08T13:14:10Z","volume":120,"author":[{"full_name":"Arslan, Feyza N","first_name":"Feyza N","last_name":"Arslan","id":"49DA7910-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5809-9566"},{"full_name":"Eckert, Julia","last_name":"Eckert","first_name":"Julia"},{"last_name":"Schmidt","first_name":"Thomas","full_name":"Schmidt, Thomas"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"12368"}]},"publication_status":"published","publisher":"Biophysical Society","department":[{"_id":"CaHe"}],"year":"2021","acknowledgement":"T.S. acknowledges funding by the research program “The Active Matter Physics of Collective Metastasis,” which is financed by the Dutch Research Council (NWO).","pmid":1,"month":"10","publication_identifier":{"issn":["0006-3495"],"eissn":["1542-0086"]},"language":[{"iso":"eng"}],"doi":"10.1016/j.bpj.2021.03.025","isi":1,"quality_controlled":"1","main_file_link":[{"url":"https://scholarlypublications.universiteitleiden.nl/access/item%3A3251048/view","open_access":"1"}],"external_id":{"pmid":["33794149"],"isi":["000704646900006"]},"oa":1,"abstract":[{"text":"Intercellular adhesion is the key to multicellularity, and its malfunction plays an important role in various developmental and disease-related processes. Although it has been intensively studied by both biologists and physicists, a commonly accepted definition of cell-cell adhesion is still being debated. Cell-cell adhesion has been described at the molecular scale as a function of adhesion receptors controlling binding affinity, at the cellular scale as resistance to detachment forces or modulation of surface tension, and at the tissue scale as a regulator of cellular rearrangements and morphogenesis. In this review, we aim to summarize and discuss recent advances in the molecular, cellular, and theoretical description of cell-cell adhesion, ranging from biomimetic models to the complexity of cells and tissues in an organismal context. In particular, we will focus on cadherin-mediated cell-cell adhesion and the role of adhesion signaling and mechanosensation therein, two processes central for understanding the biological and physical basis of cell-cell adhesion.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","status":"public","title":"Holding it together: when cadherin meets cadherin","intvolume":" 120","_id":"9350","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","day":"05","article_processing_charge":"No","scopus_import":"1","date_published":"2021-10-05T00:00:00Z","article_type":"original","page":"4182-4192","publication":"Biophysical Journal","citation":{"mla":"Arslan, Feyza N., et al. “Holding It Together: When Cadherin Meets Cadherin.” Biophysical Journal, vol. 120, Biophysical Society, 2021, pp. 4182–92, doi:10.1016/j.bpj.2021.03.025.","short":"F.N. Arslan, J. Eckert, T. Schmidt, C.-P.J. Heisenberg, Biophysical Journal 120 (2021) 4182–4192.","chicago":"Arslan, Feyza N, Julia Eckert, Thomas Schmidt, and Carl-Philipp J Heisenberg. “Holding It Together: When Cadherin Meets Cadherin.” Biophysical Journal. Biophysical Society, 2021. https://doi.org/10.1016/j.bpj.2021.03.025.","ama":"Arslan FN, Eckert J, Schmidt T, Heisenberg C-PJ. Holding it together: when cadherin meets cadherin. Biophysical Journal. 2021;120:4182-4192. doi:10.1016/j.bpj.2021.03.025","ista":"Arslan FN, Eckert J, Schmidt T, Heisenberg C-PJ. 2021. Holding it together: when cadherin meets cadherin. Biophysical Journal. 120, 4182–4192.","apa":"Arslan, F. N., Eckert, J., Schmidt, T., & Heisenberg, C.-P. J. (2021). Holding it together: when cadherin meets cadherin. Biophysical Journal. Biophysical Society. https://doi.org/10.1016/j.bpj.2021.03.025","ieee":"F. N. Arslan, J. Eckert, T. Schmidt, and C.-P. J. Heisenberg, “Holding it together: when cadherin meets cadherin,” Biophysical Journal, vol. 120. Biophysical Society, pp. 4182–4192, 2021."}},{"title":"Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesis","status":"public","ddc":["570"],"intvolume":" 12","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"10202","file":[{"access_level":"open_access","file_name":"2021_NatureComm_Pradhan.pdf","creator":"cziletti","content_type":"application/pdf","file_size":7144437,"file_id":"10262","relation":"main_file","success":1,"checksum":"c40a69ae94435ecd3a30c9874a11ef2b","date_updated":"2021-11-09T13:59:26Z","date_created":"2021-11-09T13:59:26Z"}],"oa_version":"Published Version","type":"journal_article","abstract":[{"lang":"eng","text":"Zygotic genome activation (ZGA) initiates regionalized transcription underlying distinct cellular identities. ZGA is dependent upon dynamic chromatin architecture sculpted by conserved DNA-binding proteins. However, the direct mechanistic link between the onset of ZGA and the tissue-specific transcription remains unclear. Here, we have addressed the involvement of chromatin organizer Satb2 in orchestrating both processes during zebrafish embryogenesis. Integrative analysis of transcriptome, genome-wide occupancy and chromatin accessibility reveals contrasting molecular activities of maternally deposited and zygotically synthesized Satb2. Maternal Satb2 prevents premature transcription of zygotic genes by influencing the interplay between the pluripotency factors. By contrast, zygotic Satb2 activates transcription of the same group of genes during neural crest development and organogenesis. Thus, our comparative analysis of maternal versus zygotic function of Satb2 underscores how these antithetical activities are temporally coordinated and functionally implemented highlighting the evolutionary implications of the biphasic and bimodal regulation of landmark developmental transitions by a single determinant."}],"issue":"1","article_type":"original","publication":"Nature Communications","citation":{"chicago":"Pradhan, Saurabh J., Puli Chandramouli Reddy, Michael Smutny, Ankita Sharma, Keisuke Sako, Meghana S. Oak, Rini Shah, et al. “Satb2 Acts as a Gatekeeper for Major Developmental Transitions during Early Vertebrate Embryogenesis.” Nature Communications. Springer Nature, 2021. https://doi.org/10.1038/s41467-021-26234-7.","mla":"Pradhan, Saurabh J., et al. “Satb2 Acts as a Gatekeeper for Major Developmental Transitions during Early Vertebrate Embryogenesis.” Nature Communications, vol. 12, no. 1, 6094, Springer Nature, 2021, doi:10.1038/s41467-021-26234-7.","short":"S.J. Pradhan, P.C. Reddy, M. Smutny, A. Sharma, K. Sako, M.S. Oak, R. Shah, M. Pal, O. Deshpande, G. Dsilva, Y. Tang, R. Mishra, G. Deshpande, A.J. Giraldez, M. Sonawane, C.-P.J. Heisenberg, S. Galande, Nature Communications 12 (2021).","ista":"Pradhan SJ, Reddy PC, Smutny M, Sharma A, Sako K, Oak MS, Shah R, Pal M, Deshpande O, Dsilva G, Tang Y, Mishra R, Deshpande G, Giraldez AJ, Sonawane M, Heisenberg C-PJ, Galande S. 2021. Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesis. Nature Communications. 12(1), 6094.","apa":"Pradhan, S. J., Reddy, P. C., Smutny, M., Sharma, A., Sako, K., Oak, M. S., … Galande, S. (2021). Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesis. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-021-26234-7","ieee":"S. J. Pradhan et al., “Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesis,” Nature Communications, vol. 12, no. 1. Springer Nature, 2021.","ama":"Pradhan SJ, Reddy PC, Smutny M, et al. Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesis. Nature Communications. 2021;12(1). doi:10.1038/s41467-021-26234-7"},"date_published":"2021-10-19T00:00:00Z","scopus_import":"1","day":"19","has_accepted_license":"1","article_processing_charge":"Yes","publication_status":"published","publisher":"Springer Nature","department":[{"_id":"CaHe"}],"year":"2021","acknowledgement":"We are grateful to the members of C.-P.H. and SG lab for discussions. Authors thank Shubha Tole for providing embryonic mouse tissues. Authors are grateful to Alessandro Mongera and Chetana Sachidanandan for generous help with Tg: Sox10: GFP line. Authors would like to thank Satyajeet Khare, Vanessa Barone, Jyothish S., Shalini Mishra, Yoshita Bhide, and Keshav Jha for assistance in experiments. We would also like to thank Chaitanya Dingare for valuable suggestions. We thank Diana Pinhiero and Alexandra Schauer for critical reading of early versions of the manuscript. This work was supported by the Centre of Excellence in Epigenetics program of the Department of Biotechnology, Government of India Phase I (BT/01/COE/09/07) to S.G. and R.K.M., and Phase II (BT/COE/34/SP17426/2016) to S.G. and JC Bose Fellowship (JCB/2019/000013) from Science and Engineering Research Board, Government of India to S.G., DST-BMWF Indo-Austrian bilateral program grant to S.G. and C.-P.H. The work using animal models was partly supported by the infrastructure support grants from the Department of Biotechnology (National Facility for Laboratory Model Organisms: BT/INF/22/SP17358/2016 and Establishment of a Pune Biotech Cluster, Model Organism to Human Disease: B-2 Whole Animal Imaging & Tissue Processing FacilityBT/Pune-Biocluster/01/2015). S.J.P. was supported by Fellowship from the Council of Scientific and Industrial Research, India and travel fellowship from the Company of Biologists, UK. P.C.R. was supported by the Early Career Fellowship of the Wellcome Trust-DBT India Alliance (IA/E/16/1/503057). A.S. was supported by UGC and R.S. was supported by CSIR India. M.S. was supported by core funding from the Tata Institute of Fundamental Research (TIFR 12P-121).","pmid":1,"date_updated":"2023-08-14T10:32:48Z","date_created":"2021-10-31T23:01:29Z","volume":12,"author":[{"full_name":"Pradhan, Saurabh J.","last_name":"Pradhan","first_name":"Saurabh J."},{"full_name":"Reddy, Puli Chandramouli","first_name":"Puli Chandramouli","last_name":"Reddy"},{"full_name":"Smutny, Michael","orcid":"0000-0002-5920-9090","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","last_name":"Smutny","first_name":"Michael"},{"full_name":"Sharma, Ankita","first_name":"Ankita","last_name":"Sharma"},{"id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6453-8075","first_name":"Keisuke","last_name":"Sako","full_name":"Sako, Keisuke"},{"full_name":"Oak, Meghana S.","first_name":"Meghana S.","last_name":"Oak"},{"full_name":"Shah, Rini","first_name":"Rini","last_name":"Shah"},{"first_name":"Mrinmoy","last_name":"Pal","full_name":"Pal, Mrinmoy"},{"last_name":"Deshpande","first_name":"Ojas","full_name":"Deshpande, Ojas"},{"first_name":"Greg","last_name":"Dsilva","full_name":"Dsilva, Greg"},{"full_name":"Tang, Yin","first_name":"Yin","last_name":"Tang"},{"last_name":"Mishra","first_name":"Rakesh","full_name":"Mishra, Rakesh"},{"full_name":"Deshpande, Girish","first_name":"Girish","last_name":"Deshpande"},{"full_name":"Giraldez, Antonio J.","first_name":"Antonio J.","last_name":"Giraldez"},{"first_name":"Mahendra","last_name":"Sonawane","full_name":"Sonawane, Mahendra"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Galande, Sanjeev","last_name":"Galande","first_name":"Sanjeev"}],"related_material":{"link":[{"description":"Preprint","relation":"earlier_version","url":"https://doi.org/10.1101/2020.11.23.394171 "}]},"article_number":"6094","file_date_updated":"2021-11-09T13:59:26Z","isi":1,"quality_controlled":"1","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["34667153"],"isi":["000709050300016"]},"language":[{"iso":"eng"}],"doi":"10.1038/s41467-021-26234-7","month":"10","publication_identifier":{"eissn":["20411723"]}},{"publication_identifier":{"issn":["2667-2901"]},"month":"11","external_id":{"pmid":["34800748"],"isi":["000974771600028"]},"oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.cdev.2021.203758"}],"isi":1,"quality_controlled":"1","doi":"10.1016/j.cdev.2021.203758","language":[{"iso":"eng"}],"article_number":"203758","pmid":1,"year":"2021","publisher":"Elsevier","department":[{"_id":"CaHe"}],"publication_status":"published","author":[{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Lennon, Ana Maria","first_name":"Ana Maria","last_name":"Lennon"},{"full_name":"Mayor, Roberto","first_name":"Roberto","last_name":"Mayor"},{"first_name":"Guillaume","last_name":"Salbreux","full_name":"Salbreux, Guillaume"}],"volume":168,"date_created":"2021-11-28T23:01:30Z","date_updated":"2023-08-14T13:02:40Z","scopus_import":"1","article_processing_charge":"No","day":"17","citation":{"ama":"Heisenberg C-PJ, Lennon AM, Mayor R, Salbreux G. Special rebranding issue: “Quantitative cell and developmental biology.” Cells and Development. 2021;168(12). doi:10.1016/j.cdev.2021.203758","ieee":"C.-P. J. Heisenberg, A. M. Lennon, R. Mayor, and G. Salbreux, “Special rebranding issue: ‘Quantitative cell and developmental biology,’” Cells and Development, vol. 168, no. 12. Elsevier, 2021.","apa":"Heisenberg, C.-P. J., Lennon, A. M., Mayor, R., & Salbreux, G. (2021). Special rebranding issue: “Quantitative cell and developmental biology.” Cells and Development. Elsevier. https://doi.org/10.1016/j.cdev.2021.203758","ista":"Heisenberg C-PJ, Lennon AM, Mayor R, Salbreux G. 2021. Special rebranding issue: “Quantitative cell and developmental biology”. Cells and Development. 168(12), 203758.","short":"C.-P.J. Heisenberg, A.M. Lennon, R. Mayor, G. Salbreux, Cells and Development 168 (2021).","mla":"Heisenberg, Carl-Philipp J., et al. “Special Rebranding Issue: ‘Quantitative Cell and Developmental Biology.’” Cells and Development, vol. 168, no. 12, 203758, Elsevier, 2021, doi:10.1016/j.cdev.2021.203758.","chicago":"Heisenberg, Carl-Philipp J, Ana Maria Lennon, Roberto Mayor, and Guillaume Salbreux. “Special Rebranding Issue: ‘Quantitative Cell and Developmental Biology.’” Cells and Development. Elsevier, 2021. https://doi.org/10.1016/j.cdev.2021.203758."},"publication":"Cells and Development","article_type":"letter_note","date_published":"2021-11-17T00:00:00Z","type":"journal_article","issue":"12","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"10366","intvolume":" 168","status":"public","title":"Special rebranding issue: “Quantitative cell and developmental biology”","oa_version":"Published Version"},{"isi":1,"quality_controlled":"1","project":[{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425"}],"external_id":{"isi":["000747220900010"],"pmid":["34460295"]},"language":[{"iso":"eng"}],"doi":"10.1146/annurev-genet-071819-103748","month":"08","publication_identifier":{"issn":["0066-4197"],"eissn":["1545-2948"]},"publication_status":"published","publisher":"Annual Reviews","department":[{"_id":"CaHe"}],"acknowledgement":"The authors would like to thank Feyza Nur Arslan, Suyash Naik, Diana Pinheiro, Alexandra Schauer, and Shayan Shamipour for their comments on the draft. N.M. is supported by an ISTplus postdoctoral fellowship (H2020 Marie-Sklodowska-Curie COFUND Action).","year":"2021","pmid":1,"date_updated":"2023-08-14T13:05:13Z","date_created":"2021-12-05T23:01:41Z","volume":55,"author":[{"orcid":"0000-0002-6425-5788","id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E","last_name":"Mishra","first_name":"Nikhil","full_name":"Mishra, Nikhil"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"ec_funded":1,"article_type":"original","page":"209-233","publication":"Annual Review of Genetics","citation":{"ama":"Mishra N, Heisenberg C-PJ. Dissecting organismal morphogenesis by bridging genetics and biophysics. Annual Review of Genetics. 2021;55:209-233. doi:10.1146/annurev-genet-071819-103748","ieee":"N. Mishra and C.-P. J. Heisenberg, “Dissecting organismal morphogenesis by bridging genetics and biophysics,” Annual Review of Genetics, vol. 55. Annual Reviews, pp. 209–233, 2021.","apa":"Mishra, N., & Heisenberg, C.-P. J. (2021). Dissecting organismal morphogenesis by bridging genetics and biophysics. Annual Review of Genetics. Annual Reviews. https://doi.org/10.1146/annurev-genet-071819-103748","ista":"Mishra N, Heisenberg C-PJ. 2021. Dissecting organismal morphogenesis by bridging genetics and biophysics. Annual Review of Genetics. 55, 209–233.","short":"N. Mishra, C.-P.J. Heisenberg, Annual Review of Genetics 55 (2021) 209–233.","mla":"Mishra, Nikhil, and Carl-Philipp J. Heisenberg. “Dissecting Organismal Morphogenesis by Bridging Genetics and Biophysics.” Annual Review of Genetics, vol. 55, Annual Reviews, 2021, pp. 209–33, doi:10.1146/annurev-genet-071819-103748.","chicago":"Mishra, Nikhil, and Carl-Philipp J Heisenberg. “Dissecting Organismal Morphogenesis by Bridging Genetics and Biophysics.” Annual Review of Genetics. Annual Reviews, 2021. https://doi.org/10.1146/annurev-genet-071819-103748."},"date_published":"2021-08-30T00:00:00Z","keyword":["morphogenesis","forward genetics","high-resolution microscopy","biophysics","biochemistry","patterning"],"scopus_import":"1","day":"30","article_processing_charge":"No","status":"public","title":"Dissecting organismal morphogenesis by bridging genetics and biophysics","intvolume":" 55","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"10406","oa_version":"None","type":"journal_article","abstract":[{"lang":"eng","text":"Multicellular organisms develop complex shapes from much simpler, single-celled zygotes through a process commonly called morphogenesis. Morphogenesis involves an interplay between several factors, ranging from the gene regulatory networks determining cell fate and differentiation to the mechanical processes underlying cell and tissue shape changes. Thus, the study of morphogenesis has historically been based on multidisciplinary approaches at the interface of biology with physics and mathematics. Recent technological advances have further improved our ability to study morphogenesis by bridging the gap between the genetic and biophysical factors through the development of new tools for visualizing, analyzing, and perturbing these factors and their biochemical intermediaries. Here, we review how a combination of genetic, microscopic, biophysical, and biochemical approaches has aided our attempts to understand morphogenesis and discuss potential approaches that may be beneficial to such an inquiry in the future."}]},{"project":[{"name":"Control of embryonic cleavage pattern","call_identifier":"FWF","grant_number":"I03601","_id":"2646861A-B435-11E9-9278-68D0E5697425"}],"isi":1,"quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000733610100001"]},"oa":1,"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"NanoFab"},{"_id":"Bio"}],"doi":"10.7554/eLife.75639","publication_identifier":{"eissn":["2050-084X"]},"month":"12","publisher":"eLife Sciences Publications","department":[{"_id":"CaHe"}],"publication_status":"published","year":"2021","acknowledgement":"We thank members of the Heisenberg and McDougall groups for technical advice and discussion. We are grateful to the Bioimaging and Nanofabrication facilities of IST Austria and the Imaging Platform (PIM) and animal facility (CRB) of Institut de la Mer de Villefranche (IMEV), which is supported by EMBRC-France, whose French state funds are managed by the ANR within the Investments of the Future program under reference ANR-10-INBS-0, for continuous support. This work was supported by a collaborative grant from the French Government funding agency Agence National de la Recherche to McDougall (ANR 'MorCell': ANR-17-CE 13-0028) and the Austrian Science Fund to Heisenberg (FWF: I 3601-B27).","volume":10,"date_updated":"2023-08-17T06:32:44Z","date_created":"2022-01-09T23:01:26Z","author":[{"full_name":"Godard, Benoit G","id":"33280250-F248-11E8-B48F-1D18A9856A87","first_name":"Benoit G","last_name":"Godard"},{"full_name":"Dumollard, Remi","first_name":"Remi","last_name":"Dumollard"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Mcdougall, Alex","last_name":"Mcdougall","first_name":"Alex"}],"article_number":"e75639","file_date_updated":"2022-01-10T09:40:37Z","article_type":"original","citation":{"mla":"Godard, Benoit G., et al. “Combined Effect of Cell Geometry and Polarity Domains Determines the Orientation of Unequal Division.” ELife, vol. 10, e75639, eLife Sciences Publications, 2021, doi:10.7554/eLife.75639.","short":"B.G. Godard, R. Dumollard, C.-P.J. Heisenberg, A. Mcdougall, ELife 10 (2021).","chicago":"Godard, Benoit G, Remi Dumollard, Carl-Philipp J Heisenberg, and Alex Mcdougall. “Combined Effect of Cell Geometry and Polarity Domains Determines the Orientation of Unequal Division.” ELife. eLife Sciences Publications, 2021. https://doi.org/10.7554/eLife.75639.","ama":"Godard BG, Dumollard R, Heisenberg C-PJ, Mcdougall A. Combined effect of cell geometry and polarity domains determines the orientation of unequal division. eLife. 2021;10. doi:10.7554/eLife.75639","ista":"Godard BG, Dumollard R, Heisenberg C-PJ, Mcdougall A. 2021. Combined effect of cell geometry and polarity domains determines the orientation of unequal division. eLife. 10, e75639.","apa":"Godard, B. G., Dumollard, R., Heisenberg, C.-P. J., & Mcdougall, A. (2021). Combined effect of cell geometry and polarity domains determines the orientation of unequal division. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.75639","ieee":"B. G. Godard, R. Dumollard, C.-P. J. Heisenberg, and A. Mcdougall, “Combined effect of cell geometry and polarity domains determines the orientation of unequal division,” eLife, vol. 10. eLife Sciences Publications, 2021."},"publication":"eLife","date_published":"2021-12-21T00:00:00Z","scopus_import":"1","has_accepted_license":"1","article_processing_charge":"No","day":"21","intvolume":" 10","ddc":["570"],"title":"Combined effect of cell geometry and polarity domains determines the orientation of unequal division","status":"public","_id":"10606","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file":[{"file_name":"2021_eLife_Godard.pdf","access_level":"open_access","creator":"alisjak","content_type":"application/pdf","file_size":7769934,"file_id":"10611","relation":"main_file","date_updated":"2022-01-10T09:40:37Z","date_created":"2022-01-10T09:40:37Z","success":1,"checksum":"759c7a873d554c48a6639e6350746ca6"}],"oa_version":"Published Version","type":"journal_article","abstract":[{"text":"Cell division orientation is thought to result from a competition between cell geometry and polarity domains controlling the position of the mitotic spindle during mitosis. Depending on the level of cell shape anisotropy or the strength of the polarity domain, one dominates the other and determines the orientation of the spindle. Whether and how such competition is also at work to determine unequal cell division (UCD), producing daughter cells of different size, remains unclear. Here, we show that cell geometry and polarity domains cooperate, rather than compete, in positioning the cleavage plane during UCDs in early ascidian embryos. We found that the UCDs and their orientation at the ascidian third cleavage rely on the spindle tilting in an anisotropic cell shape, and cortical polarity domains exerting different effects on spindle astral microtubules. By systematically varying mitotic cell shape, we could modulate the effect of attractive and repulsive polarity domains and consequently generate predicted daughter cell size asymmetries and position. We therefore propose that the spindle position during UCD is set by the combined activities of cell geometry and polarity domains, where cell geometry modulates the effect of cortical polarity domain(s).","lang":"eng"}]},{"external_id":{"pmid":["33321104"],"isi":["000613273900009"]},"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2020.12.002","open_access":"1"}],"oa":1,"quality_controlled":"1","isi":1,"doi":"10.1016/j.devcel.2020.12.002","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["18781551"],"issn":["15345807"]},"month":"01","pmid":1,"acknowledgement":"We would like to thank Justine Renno for illustrations and Edouard Hannezo and members of the Heisenberg group for their comments on previous versions of the manuscript.","year":"2021","publisher":"Elsevier","department":[{"_id":"CaHe"}],"publication_status":"published","related_material":{"record":[{"id":"9623","status":"public","relation":"dissertation_contains"}]},"author":[{"full_name":"Shamipour, Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Shayan","last_name":"Shamipour"},{"id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5223-3346","first_name":"Silvia","last_name":"Caballero Mancebo","full_name":"Caballero Mancebo, Silvia"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"volume":56,"date_updated":"2024-03-28T23:30:19Z","date_created":"2021-01-17T23:01:10Z","citation":{"mla":"Shamipour, Shayan, et al. “Cytoplasm’s Got Moves.” Developmental Cell, vol. 56, no. 2, Elsevier, 2021, pp. P213-226, doi:10.1016/j.devcel.2020.12.002.","short":"S. Shamipour, S. Caballero Mancebo, C.-P.J. Heisenberg, Developmental Cell 56 (2021) P213-226.","chicago":"Shamipour, Shayan, Silvia Caballero Mancebo, and Carl-Philipp J Heisenberg. “Cytoplasm’s Got Moves.” Developmental Cell. Elsevier, 2021. https://doi.org/10.1016/j.devcel.2020.12.002.","ama":"Shamipour S, Caballero Mancebo S, Heisenberg C-PJ. Cytoplasm’s got moves. Developmental Cell. 2021;56(2):P213-226. doi:10.1016/j.devcel.2020.12.002","ista":"Shamipour S, Caballero Mancebo S, Heisenberg C-PJ. 2021. Cytoplasm’s got moves. Developmental Cell. 56(2), P213-226.","ieee":"S. Shamipour, S. Caballero Mancebo, and C.-P. J. Heisenberg, “Cytoplasm’s got moves,” Developmental Cell, vol. 56, no. 2. Elsevier, pp. P213-226, 2021.","apa":"Shamipour, S., Caballero Mancebo, S., & Heisenberg, C.-P. J. (2021). Cytoplasm’s got moves. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2020.12.002"},"publication":"Developmental Cell","page":"P213-226","article_type":"original","date_published":"2021-01-25T00:00:00Z","scopus_import":"1","article_processing_charge":"No","day":"25","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9006","intvolume":" 56","status":"public","title":"Cytoplasm's got moves","oa_version":"Published Version","type":"journal_article","issue":"2","abstract":[{"text":"Cytoplasm is a gel-like crowded environment composed of various macromolecules, organelles, cytoskeletal networks, and cytosol. The structure of the cytoplasm is highly organized and heterogeneous due to the crowding of its constituents and their effective compartmentalization. In such an environment, the diffusive dynamics of the molecules are restricted, an effect that is further amplified by clustering and anchoring of molecules. Despite the crowded nature of the cytoplasm at the microscopic scale, large-scale reorganization of the cytoplasm is essential for important cellular functions, such as cell division and polarization. How such mesoscale reorganization of the cytoplasm is achieved, especially for large cells such as oocytes or syncytial tissues that can span hundreds of micrometers in size, is only beginning to be understood. In this review, we will discuss recent advances in elucidating the molecular, cellular, and biophysical mechanisms by which the cytoskeleton drives cytoplasmic reorganization across different scales, structures, and species.","lang":"eng"}]},{"month":"04","publication_identifier":{"issn":["2050-084X"]},"isi":1,"quality_controlled":"1","project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"},{"_id":"26B1E39C-B435-11E9-9278-68D0E5697425","grant_number":"25239","name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues"},{"grant_number":"ALTF 850-2017","_id":"26520D1E-B435-11E9-9278-68D0E5697425","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation"},{"_id":"266BC5CE-B435-11E9-9278-68D0E5697425","grant_number":"LT000429","name":"Coordination of mesendoderm fate specification and internalization during zebrafish gastrulation"}],"external_id":{"pmid":["32250246"],"isi":["000531544400001"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"language":[{"iso":"eng"}],"doi":"10.7554/elife.55190","article_number":"e55190","file_date_updated":"2020-07-14T12:48:04Z","ec_funded":1,"publication_status":"published","publisher":"eLife Sciences Publications","department":[{"_id":"CaHe"},{"_id":"Bio"}],"year":"2020","pmid":1,"date_created":"2020-05-25T15:01:40Z","date_updated":"2023-08-21T06:25:49Z","volume":9,"author":[{"orcid":"0000-0001-7659-9142","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","last_name":"Schauer","first_name":"Alexandra","full_name":"Schauer, Alexandra"},{"id":"2E839F16-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4333-7503","first_name":"Diana C","last_name":"Nunes Pinheiro","full_name":"Nunes Pinheiro, Diana C"},{"first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"record":[{"id":"12891","relation":"dissertation_contains","status":"public"}]},"scopus_import":"1","day":"06","article_processing_charge":"No","has_accepted_license":"1","article_type":"original","publication":"eLife","citation":{"chicago":"Schauer, Alexandra, Diana C Nunes Pinheiro, Robert Hauschild, and Carl-Philipp J Heisenberg. “Zebrafish Embryonic Explants Undergo Genetically Encoded Self-Assembly.” ELife. eLife Sciences Publications, 2020. https://doi.org/10.7554/elife.55190.","mla":"Schauer, Alexandra, et al. “Zebrafish Embryonic Explants Undergo Genetically Encoded Self-Assembly.” ELife, vol. 9, e55190, eLife Sciences Publications, 2020, doi:10.7554/elife.55190.","short":"A. Schauer, D.C. Nunes Pinheiro, R. Hauschild, C.-P.J. Heisenberg, ELife 9 (2020).","ista":"Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. 2020. Zebrafish embryonic explants undergo genetically encoded self-assembly. eLife. 9, e55190.","apa":"Schauer, A., Nunes Pinheiro, D. C., Hauschild, R., & Heisenberg, C.-P. J. (2020). Zebrafish embryonic explants undergo genetically encoded self-assembly. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.55190","ieee":"A. Schauer, D. C. Nunes Pinheiro, R. Hauschild, and C.-P. J. Heisenberg, “Zebrafish embryonic explants undergo genetically encoded self-assembly,” eLife, vol. 9. eLife Sciences Publications, 2020.","ama":"Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. Zebrafish embryonic explants undergo genetically encoded self-assembly. eLife. 2020;9. doi:10.7554/elife.55190"},"date_published":"2020-04-06T00:00:00Z","type":"journal_article","abstract":[{"text":"Embryonic stem cell cultures are thought to self-organize into embryoid bodies, able to undergo symmetry-breaking, germ layer specification and even morphogenesis. Yet, it is unclear how to reconcile this remarkable self-organization capacity with classical experiments demonstrating key roles for extrinsic biases by maternal factors and/or extraembryonic tissues in embryogenesis. Here, we show that zebrafish embryonic tissue explants, prepared prior to germ layer induction and lacking extraembryonic tissues, can specify all germ layers and form a seemingly complete mesendoderm anlage. Importantly, explant organization requires polarized inheritance of maternal factors from dorsal-marginal regions of the blastoderm. Moreover, induction of endoderm and head-mesoderm, which require peak Nodal-signaling levels, is highly variable in explants, reminiscent of embryos with reduced Nodal signals from the extraembryonic tissues. Together, these data suggest that zebrafish explants do not undergo bona fide self-organization, but rather display features of genetically encoded self-assembly, where intrinsic genetic programs control the emergence of order.","lang":"eng"}],"title":"Zebrafish embryonic explants undergo genetically encoded self-assembly","ddc":["570"],"status":"public","intvolume":" 9","_id":"7888","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file":[{"content_type":"application/pdf","file_size":7744848,"creator":"dernst","access_level":"open_access","file_name":"2020_eLife_Schauer.pdf","checksum":"f6aad884cf706846ae9357fcd728f8b5","date_created":"2020-05-25T15:15:43Z","date_updated":"2020-07-14T12:48:04Z","relation":"main_file","file_id":"7890"}],"oa_version":"Published Version"},{"language":[{"iso":"eng"}],"doi":"10.1126/science.aba6637","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"}],"isi":1,"quality_controlled":"1","oa":1,"main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/803635v1","open_access":"1"}],"external_id":{"isi":["000579169000053"]},"publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"month":"10","volume":370,"date_updated":"2023-08-22T10:36:35Z","date_created":"2020-10-19T14:09:38Z","related_material":{"link":[{"url":"https://ist.ac.at/en/news/sticking-together/","description":"News on IST Homepage","relation":"press_release"}]},"author":[{"first_name":"Tony Y.-C.","last_name":"Tsai","full_name":"Tsai, Tony Y.-C."},{"full_name":"Sikora, Mateusz K","last_name":"Sikora","first_name":"Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Xia","first_name":"Peng","orcid":"0000-0002-5419-7756","id":"4AB6C7D0-F248-11E8-B48F-1D18A9856A87","full_name":"Xia, Peng"},{"full_name":"Colak-Champollion, Tugba","first_name":"Tugba","last_name":"Colak-Champollion"},{"last_name":"Knaut","first_name":"Holger","full_name":"Knaut, Holger"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Sean G.","last_name":"Megason","full_name":"Megason, Sean G."}],"publisher":"American Association for the Advancement of Science","department":[{"_id":"CaHe"}],"publication_status":"published","year":"2020","acknowledgement":"We thank the members of the Megason and Heisenberg labs for critical discussions of and technical assistance during the work and B. Appel, S. Holley, J. Jontes, and D. Gilmour for transgenic fish. This work is supported by the Damon Runyon Cancer Foundation, a NICHD K99 fellowship (1K99HD092623), a Travelling Fellowship of the Company of Biologists, a Collaborative Research grant from the Burroughs Wellcome Foundation (T.Y.-C.T.), NIH grant 01GM107733 (T.Y.-C.T. and S.G.M.), NIH grant R01NS102322 (T.C.-C. and H.K.), and an ERC advanced grant\r\n(MECSPEC) (C.-P.H.).","ec_funded":1,"date_published":"2020-10-02T00:00:00Z","page":"113-116","article_type":"original","citation":{"ama":"Tsai TY-C, Sikora MK, Xia P, et al. An adhesion code ensures robust pattern formation during tissue morphogenesis. Science. 2020;370(6512):113-116. doi:10.1126/science.aba6637","ieee":"T. Y.-C. Tsai et al., “An adhesion code ensures robust pattern formation during tissue morphogenesis,” Science, vol. 370, no. 6512. American Association for the Advancement of Science, pp. 113–116, 2020.","apa":"Tsai, T. Y.-C., Sikora, M. K., Xia, P., Colak-Champollion, T., Knaut, H., Heisenberg, C.-P. J., & Megason, S. G. (2020). An adhesion code ensures robust pattern formation during tissue morphogenesis. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.aba6637","ista":"Tsai TY-C, Sikora MK, Xia P, Colak-Champollion T, Knaut H, Heisenberg C-PJ, Megason SG. 2020. An adhesion code ensures robust pattern formation during tissue morphogenesis. Science. 370(6512), 113–116.","short":"T.Y.-C. Tsai, M.K. Sikora, P. Xia, T. Colak-Champollion, H. Knaut, C.-P.J. Heisenberg, S.G. Megason, Science 370 (2020) 113–116.","mla":"Tsai, Tony Y. C., et al. “An Adhesion Code Ensures Robust Pattern Formation during Tissue Morphogenesis.” Science, vol. 370, no. 6512, American Association for the Advancement of Science, 2020, pp. 113–16, doi:10.1126/science.aba6637.","chicago":"Tsai, Tony Y.-C., Mateusz K Sikora, Peng Xia, Tugba Colak-Champollion, Holger Knaut, Carl-Philipp J Heisenberg, and Sean G. Megason. “An Adhesion Code Ensures Robust Pattern Formation during Tissue Morphogenesis.” Science. American Association for the Advancement of Science, 2020. https://doi.org/10.1126/science.aba6637."},"publication":"Science","article_processing_charge":"No","day":"02","keyword":["Multidisciplinary"],"scopus_import":"1","oa_version":"Preprint","intvolume":" 370","title":"An adhesion code ensures robust pattern formation during tissue morphogenesis","status":"public","_id":"8680","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","issue":"6512","abstract":[{"lang":"eng","text":"Animal development entails the organization of specific cell types in space and time, and spatial patterns must form in a robust manner. In the zebrafish spinal cord, neural progenitors form stereotypic patterns despite noisy morphogen signaling and large-scale cellular rearrangements during morphogenesis and growth. By directly measuring adhesion forces and preferences for three types of endogenous neural progenitors, we provide evidence for the differential adhesion model in which differences in intercellular adhesion mediate cell sorting. Cell type–specific combinatorial expression of different classes of cadherins (N-cadherin, cadherin 11, and protocadherin 19) results in homotypic preference ex vivo and patterning robustness in vivo. Furthermore, the differential adhesion code is regulated by the sonic hedgehog morphogen gradient. We propose that robust patterning during tissue morphogenesis results from interplay between adhesion-based self-organization and morphogen-directed patterning."}],"type":"journal_article"},{"quality_controlled":"1","isi":1,"external_id":{"pmid":["33207225"],"isi":["000600665700008"]},"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"doi":"10.1016/j.devcel.2020.10.016","publication_identifier":{"issn":["15345807"],"eissn":["18781551"]},"month":"12","publisher":"Elsevier","department":[{"_id":"CaHe"}],"publication_status":"published","pmid":1,"acknowledgement":"We thank members of the Heisenberg and McDougall groups for technical advice and discussion, Hitoyoshi Yasuo for sharing lab equipment, Lucas Leclère and Hitoyoshi Yasuo for their comments on a preliminary version of the manuscript, and Philippe Dru for the Rose plots. We are grateful to the Bioimaging and Nanofabrication facilities of IST Austria and the Imaging Platform (PIM) and animal facility (CRB) of Institut de la Mer de Villefranche (IMEV), which is supported by EMBRC-France, whose French state funds are managed by the ANR within the Investments of the Future program under reference ANR-10-INBS-0, for continuous support. This work was supported by a grant from the French Government funding agency Agence National de la Recherche (ANR “MorCell”: ANR-17-CE 13-002 8).","year":"2020","volume":55,"date_updated":"2023-08-24T11:01:22Z","date_created":"2020-12-20T23:01:19Z","related_material":{"link":[{"url":"https://ist.ac.at/en/news/relaxing-cell-divisions/","relation":"press_release","description":"News on IST Homepage"}]},"author":[{"id":"33280250-F248-11E8-B48F-1D18A9856A87","last_name":"Godard","first_name":"Benoit G","full_name":"Godard, Benoit G"},{"full_name":"Dumollard, Rémi","first_name":"Rémi","last_name":"Dumollard"},{"last_name":"Munro","first_name":"Edwin","full_name":"Munro, Edwin"},{"full_name":"Chenevert, Janet","first_name":"Janet","last_name":"Chenevert"},{"first_name":"Céline","last_name":"Hebras","full_name":"Hebras, Céline"},{"full_name":"Mcdougall, Alex","last_name":"Mcdougall","first_name":"Alex"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"page":"695-706","article_type":"original","citation":{"chicago":"Godard, Benoit G, Rémi Dumollard, Edwin Munro, Janet Chenevert, Céline Hebras, Alex Mcdougall, and Carl-Philipp J Heisenberg. “Apical Relaxation during Mitotic Rounding Promotes Tension-Oriented Cell Division.” Developmental Cell. Elsevier, 2020. https://doi.org/10.1016/j.devcel.2020.10.016.","short":"B.G. Godard, R. Dumollard, E. Munro, J. Chenevert, C. Hebras, A. Mcdougall, C.-P.J. Heisenberg, Developmental Cell 55 (2020) 695–706.","mla":"Godard, Benoit G., et al. “Apical Relaxation during Mitotic Rounding Promotes Tension-Oriented Cell Division.” Developmental Cell, vol. 55, no. 6, Elsevier, 2020, pp. 695–706, doi:10.1016/j.devcel.2020.10.016.","ieee":"B. G. Godard et al., “Apical relaxation during mitotic rounding promotes tension-oriented cell division,” Developmental Cell, vol. 55, no. 6. Elsevier, pp. 695–706, 2020.","apa":"Godard, B. G., Dumollard, R., Munro, E., Chenevert, J., Hebras, C., Mcdougall, A., & Heisenberg, C.-P. J. (2020). Apical relaxation during mitotic rounding promotes tension-oriented cell division. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2020.10.016","ista":"Godard BG, Dumollard R, Munro E, Chenevert J, Hebras C, Mcdougall A, Heisenberg C-PJ. 2020. Apical relaxation during mitotic rounding promotes tension-oriented cell division. Developmental Cell. 55(6), 695–706.","ama":"Godard BG, Dumollard R, Munro E, et al. Apical relaxation during mitotic rounding promotes tension-oriented cell division. Developmental Cell. 2020;55(6):695-706. doi:10.1016/j.devcel.2020.10.016"},"publication":"Developmental Cell","date_published":"2020-12-21T00:00:00Z","scopus_import":"1","article_processing_charge":"No","day":"21","intvolume":" 55","title":"Apical relaxation during mitotic rounding promotes tension-oriented cell division","status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"8957","oa_version":"None","type":"journal_article","issue":"6","abstract":[{"lang":"eng","text":"Global tissue tension anisotropy has been shown to trigger stereotypical cell division orientation by elongating mitotic cells along the main tension axis. Yet, how tissue tension elongates mitotic cells despite those cells undergoing mitotic rounding (MR) by globally upregulating cortical actomyosin tension remains unclear. We addressed this question by taking advantage of ascidian embryos, consisting of a small number of interphasic and mitotic blastomeres and displaying an invariant division pattern. We found that blastomeres undergo MR by locally relaxing cortical tension at their apex, thereby allowing extrinsic pulling forces from neighboring interphasic blastomeres to polarize their shape and thus division orientation. Consistently, interfering with extrinsic forces by reducing the contractility of interphasic blastomeres or disrupting the establishment of asynchronous mitotic domains leads to aberrant mitotic cell division orientations. Thus, apical relaxation during MR constitutes a key mechanism by which tissue tension anisotropy controls stereotypical cell division orientation."}]},{"scopus_import":"1","article_processing_charge":"No","day":"01","page":"343-375","citation":{"ama":"Nunes Pinheiro DC, Heisenberg C-PJ. Zebrafish gastrulation: Putting fate in motion. In: Gastrulation: From Embryonic Pattern to Form. Vol 136. Elsevier; 2020:343-375. doi:10.1016/bs.ctdb.2019.10.009","ista":"Nunes Pinheiro DC, Heisenberg C-PJ. 2020.Zebrafish gastrulation: Putting fate in motion. In: Gastrulation: From Embryonic Pattern to Form. Current Topics in Developmental Biology, vol. 136, 343–375.","apa":"Nunes Pinheiro, D. C., & Heisenberg, C.-P. J. (2020). Zebrafish gastrulation: Putting fate in motion. In Gastrulation: From Embryonic Pattern to Form (Vol. 136, pp. 343–375). Elsevier. https://doi.org/10.1016/bs.ctdb.2019.10.009","ieee":"D. C. Nunes Pinheiro and C.-P. J. Heisenberg, “Zebrafish gastrulation: Putting fate in motion,” in Gastrulation: From Embryonic Pattern to Form, vol. 136, Elsevier, 2020, pp. 343–375.","mla":"Nunes Pinheiro, Diana C., and Carl-Philipp J. Heisenberg. “Zebrafish Gastrulation: Putting Fate in Motion.” Gastrulation: From Embryonic Pattern to Form, vol. 136, Elsevier, 2020, pp. 343–75, doi:10.1016/bs.ctdb.2019.10.009.","short":"D.C. Nunes Pinheiro, C.-P.J. Heisenberg, in:, Gastrulation: From Embryonic Pattern to Form, Elsevier, 2020, pp. 343–375.","chicago":"Nunes Pinheiro, Diana C, and Carl-Philipp J Heisenberg. “Zebrafish Gastrulation: Putting Fate in Motion.” In Gastrulation: From Embryonic Pattern to Form, 136:343–75. Elsevier, 2020. https://doi.org/10.1016/bs.ctdb.2019.10.009."},"publication":"Gastrulation: From Embryonic Pattern to Form","date_published":"2020-06-01T00:00:00Z","alternative_title":["Current Topics in Developmental Biology"],"type":"book_chapter","abstract":[{"lang":"eng","text":"Gastrulation entails specification and formation of three embryonic germ layers—ectoderm, mesoderm and endoderm—thereby establishing the basis for the future body plan. In zebrafish embryos, germ layer specification occurs during blastula and early gastrula stages (Ho & Kimmel, 1993), a period when the main morphogenetic movements underlying gastrulation are initiated. Hence, the signals driving progenitor cell fate specification, such as Nodal ligands from the TGF-β family, also play key roles in regulating germ layer progenitor cell segregation (Carmany-Rampey & Schier, 2001; David & Rosa, 2001; Feldman et al., 2000; Gritsman et al., 1999; Keller et al., 2008). In this review, we summarize and discuss the main signaling pathways involved in germ layer progenitor cell fate specification and segregation, specifically focusing on recent advances in understanding the interplay between mesoderm and endoderm specification and the internalization movements at the onset of zebrafish gastrulation."}],"intvolume":" 136","title":"Zebrafish gastrulation: Putting fate in motion","status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"7227","oa_version":"None","publication_identifier":{"issn":["00702153"]},"month":"06","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"name":"Control of embryonic cleavage pattern","call_identifier":"FWF","grant_number":"I03601","_id":"2646861A-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Control of epithelial cell layer spreading in zebrafish","grant_number":"I03196","_id":"2608FC64-B435-11E9-9278-68D0E5697425"},{"grant_number":"LT000429","_id":"266BC5CE-B435-11E9-9278-68D0E5697425","name":"Coordination of mesendoderm fate specification and internalization during zebrafish gastrulation"},{"_id":"26520D1E-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 850-2017","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation"}],"quality_controlled":"1","isi":1,"external_id":{"pmid":["31959295"],"isi":["000611830600013"]},"language":[{"iso":"eng"}],"doi":"10.1016/bs.ctdb.2019.10.009","ec_funded":1,"department":[{"_id":"CaHe"}],"publisher":"Elsevier","publication_status":"published","pmid":1,"acknowledgement":"We thank Alexandra Schauer, Nicoletta Petridou and Feyza Nur Arslan for comments on the manuscript. Research in the Heisenberg laboratory is supported by an ERC Advanced Grant (MECSPEC 742573), ANR/FWF (I03601) and FWF/DFG (I03196) International Cooperation Grants. D. Pinheiro acknowledges a fellowship from EMBO ALTF (850-2017) and is currently supported by HFSP LTF (LT000429/2018-L2).","year":"2020","volume":136,"date_created":"2020-01-05T23:00:46Z","date_updated":"2023-09-06T14:54:36Z","author":[{"last_name":"Nunes Pinheiro","first_name":"Diana C","orcid":"0000-0003-4333-7503","id":"2E839F16-F248-11E8-B48F-1D18A9856A87","full_name":"Nunes Pinheiro, Diana C"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}]},{"author":[{"last_name":"Bruce","first_name":"Ashley E.E.","full_name":"Bruce, Ashley E.E."},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2020-01-30T09:24:06Z","date_updated":"2024-02-22T13:23:09Z","volume":136,"year":"2020","publication_status":"published","publisher":"Elsevier","department":[{"_id":"CaHe"}],"editor":[{"last_name":"Solnica-Krezel","first_name":"Lilianna ","full_name":"Solnica-Krezel, Lilianna "}],"month":"01","publication_identifier":{"isbn":["9780128127988"],"issn":["0070-2153"]},"doi":"10.1016/bs.ctdb.2019.07.001","language":[{"iso":"eng"}],"external_id":{"isi":["000611830600012"]},"quality_controlled":"1","isi":1,"abstract":[{"lang":"eng","text":"Epiboly is a conserved gastrulation movement describing the thinning and spreading of a sheet or multi-layer of cells. The zebrafish embryo has emerged as a vital model system to address the cellular and molecular mechanisms that drive epiboly. In the zebrafish embryo, the blastoderm, consisting of a simple squamous epithelium (the enveloping layer) and an underlying mass of deep cells, as well as a yolk nuclear syncytium (the yolk syncytial layer) undergo epiboly to internalize the yolk cell during gastrulation. The major events during zebrafish epiboly are: expansion of the enveloping layer and the internal yolk syncytial layer, reduction and removal of the yolk membrane ahead of the advancing blastoderm margin and deep cell rearrangements between the enveloping layer and yolk syncytial layer to thin the blastoderm. Here, work addressing the cellular and molecular mechanisms as well as the sources of the mechanical forces that underlie these events is reviewed. The contribution of recent findings to the current model of epiboly as well as open questions and future prospects are also discussed."}],"type":"book_chapter","oa_version":"None","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"7410","status":"public","title":"Mechanisms of zebrafish epiboly: A current view","intvolume":" 136","day":"01","article_processing_charge":"No","scopus_import":"1","series_title":"Current Topics in Developmental Biology","date_published":"2020-01-01T00:00:00Z","publication":"Gastrulation: From Embryonic Pattern to Form","citation":{"chicago":"Bruce, Ashley E.E., and Carl-Philipp J Heisenberg. “Mechanisms of Zebrafish Epiboly: A Current View.” In Gastrulation: From Embryonic Pattern to Form, edited by Lilianna Solnica-Krezel, 136:319–41. Current Topics in Developmental Biology. Elsevier, 2020. https://doi.org/10.1016/bs.ctdb.2019.07.001.","short":"A.E.E. Bruce, C.-P.J. Heisenberg, in:, L. Solnica-Krezel (Ed.), Gastrulation: From Embryonic Pattern to Form, Elsevier, 2020, pp. 319–341.","mla":"Bruce, Ashley E. E., and Carl-Philipp J. Heisenberg. “Mechanisms of Zebrafish Epiboly: A Current View.” Gastrulation: From Embryonic Pattern to Form, edited by Lilianna Solnica-Krezel, vol. 136, Elsevier, 2020, pp. 319–41, doi:10.1016/bs.ctdb.2019.07.001.","apa":"Bruce, A. E. E., & Heisenberg, C.-P. J. (2020). Mechanisms of zebrafish epiboly: A current view. In L. Solnica-Krezel (Ed.), Gastrulation: From Embryonic Pattern to Form (Vol. 136, pp. 319–341). Elsevier. https://doi.org/10.1016/bs.ctdb.2019.07.001","ieee":"A. E. E. Bruce and C.-P. J. Heisenberg, “Mechanisms of zebrafish epiboly: A current view,” in Gastrulation: From Embryonic Pattern to Form, vol. 136, L. Solnica-Krezel, Ed. Elsevier, 2020, pp. 319–341.","ista":"Bruce AEE, Heisenberg C-PJ. 2020.Mechanisms of zebrafish epiboly: A current view. In: Gastrulation: From Embryonic Pattern to Form. vol. 136, 319–341.","ama":"Bruce AEE, Heisenberg C-PJ. Mechanisms of zebrafish epiboly: A current view. In: Solnica-Krezel L, ed. Gastrulation: From Embryonic Pattern to Form. Vol 136. Current Topics in Developmental Biology. Elsevier; 2020:319-341. doi:10.1016/bs.ctdb.2019.07.001"},"page":"319-341"},{"ec_funded":1,"abstract":[{"lang":"eng","text":"Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level of cortical tension outside of the cell-cell contact, when pulling at the contact edge, scales with the total size to which a cell-cell contact can grow1,2. Here we show in zebrafish primary germ layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase, and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell-cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. Once tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell-cell contact size is limited by tension stabilizing E-cadherin-actin complexes at the contact."}],"type":"preprint","related_material":{"record":[{"relation":"later_version","status":"public","id":"10766"},{"status":"public","relation":"dissertation_contains","id":"9623"}]},"author":[{"full_name":"Slovakova, Jana","id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87","first_name":"Jana","last_name":"Slovakova"},{"full_name":"Sikora, Mateusz K","last_name":"Sikora","first_name":"Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Caballero Mancebo, Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5223-3346","first_name":"Silvia","last_name":"Caballero Mancebo"},{"orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","first_name":"Gabriel","full_name":"Krens, Gabriel"},{"full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann","first_name":"Walter"},{"full_name":"Huljev, Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","first_name":"Karla","last_name":"Huljev"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"oa_version":"Preprint","date_created":"2021-07-29T11:29:50Z","date_updated":"2024-03-28T23:30:19Z","acknowledgement":"We would like to thank Edouard Hannezo for discussions, Shayan Shami Pour and Daniel Capek for help with data analysis, Vanessa Barone and other members of the Heisenberg laboratory for thoughtful discussions and comments on the manuscript. We also thank Jack Merrin for preparing the microwells, and the Scientific Service Units at IST Austria, specifically Bioimaging and Electron Microscopy, and the Zebrafish Facility for continuous support. We acknowledge Hitoshi Morita for the kind gift of VinculinB-GFP plasmid. This research was supported by an ERC Advanced Grant (MECSPEC) to C.-P.H, EMBO Long Term grant (ALTF 187-2013) to M.S and IST Fellow Marie-Curie COFUND No. P_IST_EU01 to J.S.","_id":"9750","year":"2020","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","department":[{"_id":"CaHe"},{"_id":"EM-Fac"},{"_id":"Bio"}],"publisher":"Cold Spring Harbor Laboratory","title":"Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion","publication_status":"published","status":"public","article_processing_charge":"No","day":"20","month":"11","date_published":"2020-11-20T00:00:00Z","doi":"10.1101/2020.11.20.391284","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"SSU"}],"oa":1,"citation":{"short":"J. Slovakova, M.K. Sikora, S. Caballero Mancebo, G. Krens, W. Kaufmann, K. Huljev, C.-P.J. Heisenberg, BioRxiv (2020).","mla":"Slovakova, Jana, et al. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” BioRxiv, Cold Spring Harbor Laboratory, 2020, doi:10.1101/2020.11.20.391284.","chicago":"Slovakova, Jana, Mateusz K Sikora, Silvia Caballero Mancebo, Gabriel Krens, Walter Kaufmann, Karla Huljev, and Carl-Philipp J Heisenberg. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” BioRxiv. Cold Spring Harbor Laboratory, 2020. https://doi.org/10.1101/2020.11.20.391284.","ama":"Slovakova J, Sikora MK, Caballero Mancebo S, et al. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv. 2020. doi:10.1101/2020.11.20.391284","apa":"Slovakova, J., Sikora, M. K., Caballero Mancebo, S., Krens, G., Kaufmann, W., Huljev, K., & Heisenberg, C.-P. J. (2020). Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.11.20.391284","ieee":"J. Slovakova et al., “Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion,” bioRxiv. Cold Spring Harbor Laboratory, 2020.","ista":"Slovakova J, Sikora MK, Caballero Mancebo S, Krens G, Kaufmann W, Huljev K, Heisenberg C-PJ. 2020. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv, 10.1101/2020.11.20.391284."},"main_file_link":[{"url":"https://doi.org/10.1101/2020.11.20.391284","open_access":"1"}],"publication":"bioRxiv","page":"41","project":[{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"},{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"},{"name":"Modulation of adhesion function in cell-cell contact formation by cortical tension","_id":"2521E28E-B435-11E9-9278-68D0E5697425","grant_number":"187-2013"}]},{"scopus_import":1,"series_title":"Methods in Molecular Biology","publication_identifier":{"isbn":["978-1-4939-8909-6"]},"day":"01","month":"01","citation":{"mla":"Asaoka, Yoichi, et al. “Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids.” The Hippo Pathway, edited by Alexander Hergovich, vol. 1893, Springer, 2019, pp. 167–81, doi:10.1007/978-1-4939-8910-2_14.","short":"Y. Asaoka, H. Morita, H. Furumoto, C.-P.J. Heisenberg, M. Furutani-Seiki, in:, A. Hergovich (Ed.), The Hippo Pathway, Springer, 2019, pp. 167–181.","chicago":"Asaoka, Yoichi, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp J Heisenberg, and Makoto Furutani-Seiki. “Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids.” In The Hippo Pathway, edited by Alexander Hergovich, 1893:167–81. Methods in Molecular Biology. Springer, 2019. https://doi.org/10.1007/978-1-4939-8910-2_14.","ama":"Asaoka Y, Morita H, Furumoto H, Heisenberg C-PJ, Furutani-Seiki M. Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids. In: Hergovich A, ed. The Hippo Pathway. Vol 1893. Methods in Molecular Biology. Springer; 2019:167-181. doi:10.1007/978-1-4939-8910-2_14","ista":"Asaoka Y, Morita H, Furumoto H, Heisenberg C-PJ, Furutani-Seiki M. 2019.Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids. In: The hippo pathway. MIMB, vol. 1893, 167–181.","apa":"Asaoka, Y., Morita, H., Furumoto, H., Heisenberg, C.-P. J., & Furutani-Seiki, M. (2019). Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids. In A. Hergovich (Ed.), The hippo pathway (Vol. 1893, pp. 167–181). Springer. https://doi.org/10.1007/978-1-4939-8910-2_14","ieee":"Y. Asaoka, H. Morita, H. Furumoto, C.-P. J. Heisenberg, and M. Furutani-Seiki, “Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids,” in The hippo pathway, vol. 1893, A. Hergovich, Ed. Springer, 2019, pp. 167–181."},"publication":"The hippo pathway","page":"167-181","quality_controlled":"1","doi":"10.1007/978-1-4939-8910-2_14","date_published":"2019-01-01T00:00:00Z","language":[{"iso":"eng"}],"type":"book_chapter","alternative_title":["MIMB"],"abstract":[{"lang":"eng","text":"The transcription coactivator, Yes-associated protein (YAP), which is a nuclear effector of the Hippo signaling pathway, has been shown to be a mechano-transducer. By using mutant fish and human 3D spheroids, we have recently demonstrated that YAP is also a mechano-effector. YAP functions in three-dimensional (3D) morphogenesis of organ and global body shape by controlling actomyosin-mediated tissue tension. In this chapter, we present a platform that links the findings in fish embryos with human cells. The protocols for analyzing tissue tension-mediated global body shape/organ morphogenesis in vivo and ex vivo using medaka fish embryos and in vitro using human cell spheroids represent useful tools for unraveling the molecular mechanisms by which YAP functions in regulating global body/organ morphogenesis."}],"_id":"5793","year":"2019","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","editor":[{"full_name":"Hergovich, Alexander","first_name":"Alexander","last_name":"Hergovich"}],"intvolume":" 1893","department":[{"_id":"CaHe"}],"publisher":"Springer","status":"public","publication_status":"published","title":"Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids","author":[{"last_name":"Asaoka","first_name":"Yoichi","full_name":"Asaoka, Yoichi"},{"full_name":"Morita, Hitoshi","first_name":"Hitoshi","last_name":"Morita"},{"last_name":"Furumoto","first_name":"Hiroko","full_name":"Furumoto, Hiroko"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"},{"last_name":"Furutani-Seiki","first_name":"Makoto","full_name":"Furutani-Seiki, Makoto"}],"oa_version":"None","volume":1893,"date_updated":"2021-01-12T08:03:30Z","date_created":"2019-01-06T22:59:11Z"},{"month":"02","external_id":{"isi":["000458025300001"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"}],"quality_controlled":"1","isi":1,"doi":"10.7554/eLife.42093","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"article_number":"e42093","ec_funded":1,"file_date_updated":"2020-07-14T12:47:17Z","year":"2019","department":[{"_id":"CaHe"},{"_id":"HaJa"}],"publisher":"eLife Sciences Publications","publication_status":"published","author":[{"first_name":"Daniel","last_name":"Capek","id":"31C42484-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5199-9940","full_name":"Capek, Daniel"},{"last_name":"Smutny","first_name":"Michael","orcid":"0000-0002-5920-9090","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","full_name":"Smutny, Michael"},{"full_name":"Tichy, Alexandra Madelaine","first_name":"Alexandra Madelaine","last_name":"Tichy"},{"full_name":"Morri, Maurizio","first_name":"Maurizio","last_name":"Morri","id":"4863116E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Janovjak, Harald L","orcid":"0000-0002-8023-9315","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","last_name":"Janovjak","first_name":"Harald L"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"volume":8,"date_updated":"2023-08-24T14:46:01Z","date_created":"2019-02-17T22:59:22Z","scopus_import":"1","has_accepted_license":"1","article_processing_charge":"No","day":"06","citation":{"short":"D. Capek, M. Smutny, A.M. Tichy, M. Morri, H.L. Janovjak, C.-P.J. Heisenberg, ELife 8 (2019).","mla":"Capek, Daniel, et al. “Light-Activated Frizzled7 Reveals a Permissive Role of Non-Canonical Wnt Signaling in Mesendoderm Cell Migration.” ELife, vol. 8, e42093, eLife Sciences Publications, 2019, doi:10.7554/eLife.42093.","chicago":"Capek, Daniel, Michael Smutny, Alexandra Madelaine Tichy, Maurizio Morri, Harald L Janovjak, and Carl-Philipp J Heisenberg. “Light-Activated Frizzled7 Reveals a Permissive Role of Non-Canonical Wnt Signaling in Mesendoderm Cell Migration.” ELife. eLife Sciences Publications, 2019. https://doi.org/10.7554/eLife.42093.","ama":"Capek D, Smutny M, Tichy AM, Morri M, Janovjak HL, Heisenberg C-PJ. Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. eLife. 2019;8. doi:10.7554/eLife.42093","ieee":"D. Capek, M. Smutny, A. M. Tichy, M. Morri, H. L. Janovjak, and C.-P. J. Heisenberg, “Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration,” eLife, vol. 8. eLife Sciences Publications, 2019.","apa":"Capek, D., Smutny, M., Tichy, A. M., Morri, M., Janovjak, H. L., & Heisenberg, C.-P. J. (2019). Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.42093","ista":"Capek D, Smutny M, Tichy AM, Morri M, Janovjak HL, Heisenberg C-PJ. 2019. Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. eLife. 8, e42093."},"publication":"eLife","date_published":"2019-02-06T00:00:00Z","type":"journal_article","abstract":[{"lang":"eng","text":"Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation."}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6025","intvolume":" 8","status":"public","ddc":["570"],"title":"Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration","oa_version":"Published Version","file":[{"access_level":"open_access","file_name":"2019_elife_Capek.pdf","creator":"dernst","file_size":5500707,"content_type":"application/pdf","file_id":"6041","relation":"main_file","checksum":"6cb4ca6d4aa96f6f187a5983aa3e660a","date_updated":"2020-07-14T12:47:17Z","date_created":"2019-02-18T15:17:21Z"}]},{"oa_version":"Published Version","intvolume":" 176","status":"public","title":"Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6087","issue":"6","abstract":[{"lang":"eng","text":"Cell fate specification by lateral inhibition typically involves contact signaling through the Delta-Notch signaling pathway. However, whether this is the only signaling mode mediating lateral inhibition remains unclear. Here we show that in zebrafish oogenesis, a group of cells within the granulosa cell layer at the oocyte animal pole acquire elevated levels of the transcriptional coactivator TAZ in their nuclei. One of these cells, the future micropyle precursor cell (MPC), accumulates increasingly high levels of nuclear TAZ and grows faster than its surrounding cells, mechanically compressing those cells, which ultimately lose TAZ from their nuclei. Strikingly, relieving neighbor-cell compression by MPC ablation or aspiration restores nuclear TAZ accumulation in neighboring cells, eventually leading to MPC re-specification from these cells. Conversely, MPC specification is defective in taz−/− follicles. These findings uncover a novel mode of lateral inhibition in cell fate specification based on mechanical signals controlling TAZ activity."}],"type":"journal_article","date_published":"2019-03-07T00:00:00Z","page":"1379-1392.e14","article_type":"original","citation":{"ama":"Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. 2019;176(6):1379-1392.e14. doi:10.1016/j.cell.2019.01.019","ieee":"P. Xia, D. J. Gütl, V. Zheden, and C.-P. J. Heisenberg, “Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity,” Cell, vol. 176, no. 6. Elsevier, p. 1379–1392.e14, 2019.","apa":"Xia, P., Gütl, D. J., Zheden, V., & Heisenberg, C.-P. J. (2019). Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.01.019","ista":"Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. 2019. Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. 176(6), 1379–1392.e14.","short":"P. Xia, D.J. Gütl, V. Zheden, C.-P.J. Heisenberg, Cell 176 (2019) 1379–1392.e14.","mla":"Xia, Peng, et al. “Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.” Cell, vol. 176, no. 6, Elsevier, 2019, p. 1379–1392.e14, doi:10.1016/j.cell.2019.01.019.","chicago":"Xia, Peng, Daniel J Gütl, Vanessa Zheden, and Carl-Philipp J Heisenberg. “Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.01.019."},"publication":"Cell","article_processing_charge":"No","day":"07","scopus_import":"1","volume":176,"date_updated":"2023-08-25T08:02:23Z","date_created":"2019-03-10T22:59:19Z","related_material":{"link":[{"url":"https://ist.ac.at/en/news/in-zebrafish-eggs-most-rapidly-growing-cell-inhibits-its-neighbours-through-mechanical-signals/","relation":"press_release","description":"News on IST Homepage"}]},"author":[{"full_name":"Xia, Peng","last_name":"Xia","first_name":"Peng","orcid":"0000-0002-5419-7756","id":"4AB6C7D0-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Gütl, Daniel J","first_name":"Daniel J","last_name":"Gütl","id":"381929CE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Vanessa","last_name":"Zheden","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"department":[{"_id":"CaHe"},{"_id":"EM-Fac"}],"publisher":"Elsevier","publication_status":"published","pmid":1,"year":"2019","acknowledgement":"We thank Roland Dosch, Makoto Furutani-Seiki, Brian Link, Mary Mullins, and Masazumi Tada for providing transgenic and/or mutant zebrafish lines; Alexandra Schauer, Shayan Shami-Pour, and the rest of the Heisenberg lab for technical assistance and feedback on the manuscript; and the Bioimaging, Electron Microscopy, and Zebrafish facilities of IST Austria for continuous support. This work was supported by an ERC advanced grant ( MECSPEC to C.-P.H.).","ec_funded":1,"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"doi":"10.1016/j.cell.2019.01.019","project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"}],"isi":1,"quality_controlled":"1","external_id":{"pmid":["30773315"],"isi":["000460509600013"]},"oa":1,"main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2019.01.019","open_access":"1"}],"month":"03"},{"ec_funded":1,"pmid":1,"year":"2019","publisher":"Elsevier","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"publication_status":"published","author":[{"first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"volume":178,"date_created":"2019-06-30T21:59:11Z","date_updated":"2023-08-28T12:25:21Z","publication_identifier":{"issn":["00928674"]},"month":"07","external_id":{"isi":["000473002700005"],"pmid":["31251912"]},"oa":1,"main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2019.05.052","open_access":"1"}],"project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020"},{"call_identifier":"FWF","name":"Active mechano-chemical description of the cell cytoskeleton","_id":"268294B6-B435-11E9-9278-68D0E5697425","grant_number":"P31639"}],"quality_controlled":"1","isi":1,"doi":"10.1016/j.cell.2019.05.052","language":[{"iso":"eng"}],"type":"journal_article","issue":"1","abstract":[{"lang":"eng","text":"There is increasing evidence that both mechanical and biochemical signals play important roles in development and disease. The development of complex organisms, in particular, has been proposed to rely on the feedback between mechanical and biochemical patterning events. This feedback occurs at the molecular level via mechanosensation but can also arise as an emergent property of the system at the cellular and tissue level. In recent years, dynamic changes in tissue geometry, flow, rheology, and cell fate specification have emerged as key platforms of mechanochemical feedback loops in multiple processes. Here, we review recent experimental and theoretical advances in understanding how these feedbacks function in development and disease."}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6601","intvolume":" 178","title":"Mechanochemical feedback loops in development and disease","status":"public","oa_version":"Published Version","scopus_import":"1","article_processing_charge":"No","day":"27","citation":{"ama":"Hannezo EB, Heisenberg C-PJ. Mechanochemical feedback loops in development and disease. Cell. 2019;178(1):12-25. doi:10.1016/j.cell.2019.05.052","ista":"Hannezo EB, Heisenberg C-PJ. 2019. Mechanochemical feedback loops in development and disease. Cell. 178(1), 12–25.","ieee":"E. B. Hannezo and C.-P. J. Heisenberg, “Mechanochemical feedback loops in development and disease,” Cell, vol. 178, no. 1. Elsevier, pp. 12–25, 2019.","apa":"Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Mechanochemical feedback loops in development and disease. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.05.052","mla":"Hannezo, Edouard B., and Carl-Philipp J. Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” Cell, vol. 178, no. 1, Elsevier, 2019, pp. 12–25, doi:10.1016/j.cell.2019.05.052.","short":"E.B. Hannezo, C.-P.J. Heisenberg, Cell 178 (2019) 12–25.","chicago":"Hannezo, Edouard B, and Carl-Philipp J Heisenberg. “Mechanochemical Feedback Loops in Development and Disease.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.05.052."},"publication":"Cell","page":"12-25","article_type":"review","date_published":"2019-07-27T00:00:00Z"},{"abstract":[{"text":"The spatiotemporal organization of cell divisions constitutes an integral part in the development of multicellular organisms, and mis-regulation of cell divisions can lead to severe developmental defects. Cell divisions have an important morphogenetic function in development by regulating growth and shape acquisition of developing tissues, and, conversely, tissue morphogenesis is known to affect both the rate and orientation of cell divisions. Moreover, cell divisions are associated with an extensive reorganization of the cytoskeleton and adhesion apparatus in the dividing cells that in turn can affect large-scale tissue rheological properties. Thus, the interplay between cell divisions and tissue morphogenesis plays a key role in embryo and tissue morphogenesis.","lang":"eng"}],"type":"journal_article","author":[{"first_name":"Benoit G","last_name":"Godard","id":"33280250-F248-11E8-B48F-1D18A9856A87","full_name":"Godard, Benoit G"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"volume":60,"oa_version":"None","date_updated":"2023-08-29T06:33:14Z","date_created":"2019-07-14T21:59:17Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6631","year":"2019","publisher":"Elsevier","intvolume":" 60","department":[{"_id":"CaHe"}],"status":"public","publication_status":"published","title":"Cell division and tissue mechanics","article_processing_charge":"No","publication_identifier":{"issn":["0955-0674"]},"month":"10","day":"01","scopus_import":"1","date_published":"2019-10-01T00:00:00Z","doi":"10.1016/j.ceb.2019.05.007","language":[{"iso":"eng"}],"external_id":{"isi":["000486545800016"]},"citation":{"mla":"Godard, Benoit G., and Carl-Philipp J. Heisenberg. “Cell Division and Tissue Mechanics.” Current Opinion in Cell Biology, vol. 60, Elsevier, 2019, pp. 114–20, doi:10.1016/j.ceb.2019.05.007.","short":"B.G. Godard, C.-P.J. Heisenberg, Current Opinion in Cell Biology 60 (2019) 114–120.","chicago":"Godard, Benoit G, and Carl-Philipp J Heisenberg. “Cell Division and Tissue Mechanics.” Current Opinion in Cell Biology. Elsevier, 2019. https://doi.org/10.1016/j.ceb.2019.05.007.","ama":"Godard BG, Heisenberg C-PJ. Cell division and tissue mechanics. Current Opinion in Cell Biology. 2019;60:114-120. doi:10.1016/j.ceb.2019.05.007","ista":"Godard BG, Heisenberg C-PJ. 2019. Cell division and tissue mechanics. Current Opinion in Cell Biology. 60, 114–120.","ieee":"B. G. Godard and C.-P. J. Heisenberg, “Cell division and tissue mechanics,” Current Opinion in Cell Biology, vol. 60. Elsevier, pp. 114–120, 2019.","apa":"Godard, B. G., & Heisenberg, C.-P. J. (2019). Cell division and tissue mechanics. Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2019.05.007"},"publication":"Current Opinion in Cell Biology","page":"114-120","quality_controlled":"1","isi":1},{"date_published":"2019-08-01T00:00:00Z","citation":{"ama":"Tavano S, Heisenberg C-PJ. Migrasomes take center stage. Nature Cell Biology. 2019;21(8):918-920. doi:10.1038/s41556-019-0369-3","apa":"Tavano, S., & Heisenberg, C.-P. J. (2019). Migrasomes take center stage. Nature Cell Biology. Springer Nature. https://doi.org/10.1038/s41556-019-0369-3","ieee":"S. Tavano and C.-P. J. Heisenberg, “Migrasomes take center stage,” Nature Cell Biology, vol. 21, no. 8. Springer Nature, pp. 918–920, 2019.","ista":"Tavano S, Heisenberg C-PJ. 2019. Migrasomes take center stage. Nature Cell Biology. 21(8), 918–920.","short":"S. Tavano, C.-P.J. Heisenberg, Nature Cell Biology 21 (2019) 918–920.","mla":"Tavano, Ste, and Carl-Philipp J. Heisenberg. “Migrasomes Take Center Stage.” Nature Cell Biology, vol. 21, no. 8, Springer Nature, 2019, pp. 918–20, doi:10.1038/s41556-019-0369-3.","chicago":"Tavano, Ste, and Carl-Philipp J Heisenberg. “Migrasomes Take Center Stage.” Nature Cell Biology. Springer Nature, 2019. https://doi.org/10.1038/s41556-019-0369-3."},"publication":"Nature Cell Biology","page":"918-920","article_processing_charge":"No","day":"01","scopus_import":"1","oa_version":"None","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6837","intvolume":" 21","title":"Migrasomes take center stage","status":"public","issue":"8","abstract":[{"text":"Migrasomes are a recently discovered type of extracellular vesicles that are characteristically generated along retraction fibers in migrating cells. Two studies now show how migrasomes are formed and how they function in the physiologically relevant context of the developing zebrafish embryo.","lang":"eng"}],"type":"journal_article","doi":"10.1038/s41556-019-0369-3","language":[{"iso":"eng"}],"external_id":{"pmid":["31371826"],"isi":["000478029000003"]},"quality_controlled":"1","isi":1,"publication_identifier":{"eissn":["1476-4679"]},"month":"08","author":[{"full_name":"Tavano, Ste","last_name":"Tavano","first_name":"Ste","orcid":"0000-0001-9970-7804","id":"2F162F0C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"volume":21,"date_updated":"2023-08-29T07:42:20Z","date_created":"2019-09-01T22:00:57Z","pmid":1,"year":"2019","publisher":"Springer Nature","department":[{"_id":"CaHe"}],"publication_status":"published"},{"citation":{"ista":"Bornhorst D, Xia P, Nakajima H, Dingare C, Herzog W, Lecaudey V, Mochizuki N, Heisenberg C-PJ, Yelon D, Abdelilah-Seyfried S. 2019. Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions. Nature communications. 10(1), 4113.","apa":"Bornhorst, D., Xia, P., Nakajima, H., Dingare, C., Herzog, W., Lecaudey, V., … Abdelilah-Seyfried, S. (2019). Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/s41467-019-12068-x","ieee":"D. Bornhorst et al., “Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions,” Nature communications, vol. 10, no. 1. Nature Publishing Group, p. 4113, 2019.","ama":"Bornhorst D, Xia P, Nakajima H, et al. Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions. Nature communications. 2019;10(1):4113. doi:10.1038/s41467-019-12068-x","chicago":"Bornhorst, Dorothee, Peng Xia, Hiroyuki Nakajima, Chaitanya Dingare, Wiebke Herzog, Virginie Lecaudey, Naoki Mochizuki, Carl-Philipp J Heisenberg, Deborah Yelon, and Salim Abdelilah-Seyfried. “Biomechanical Signaling within the Developing Zebrafish Heart Attunes Endocardial Growth to Myocardial Chamber Dimensions.” Nature Communications. Nature Publishing Group, 2019. https://doi.org/10.1038/s41467-019-12068-x.","mla":"Bornhorst, Dorothee, et al. “Biomechanical Signaling within the Developing Zebrafish Heart Attunes Endocardial Growth to Myocardial Chamber Dimensions.” Nature Communications, vol. 10, no. 1, Nature Publishing Group, 2019, p. 4113, doi:10.1038/s41467-019-12068-x.","short":"D. Bornhorst, P. Xia, H. Nakajima, C. Dingare, W. Herzog, V. Lecaudey, N. Mochizuki, C.-P.J. Heisenberg, D. Yelon, S. Abdelilah-Seyfried, Nature Communications 10 (2019) 4113."},"publication":"Nature communications","page":"4113","date_published":"2019-09-11T00:00:00Z","scopus_import":"1","has_accepted_license":"1","article_processing_charge":"No","day":"11","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6899","intvolume":" 10","ddc":["570"],"title":"Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions","status":"public","file":[{"relation":"main_file","file_id":"6926","checksum":"62c2512712e16d27c1797d318d14ba9f","date_created":"2019-10-01T11:18:50Z","date_updated":"2020-07-14T12:47:44Z","access_level":"open_access","file_name":"2019_Nature_Bornhorst.pdf","content_type":"application/pdf","file_size":3905793,"creator":"kschuh"}],"oa_version":"Published Version","type":"journal_article","issue":"1","abstract":[{"lang":"eng","text":"Intra-organ communication guides morphogenetic processes that are essential for an organ to carry out complex physiological functions. In the heart, the growth of the myocardium is tightly coupled to that of the endocardium, a specialized endothelial tissue that lines its interior. Several molecular pathways have been implicated in the communication between these tissues including secreted factors, components of the extracellular matrix, or proteins involved in cell-cell communication. Yet, it is unknown how the growth of the endocardium is coordinated with that of the myocardium. Here, we show that an increased expansion of the myocardial atrial chamber volume generates higher junctional forces within endocardial cells. This leads to biomechanical signaling involving VE-cadherin, triggering nuclear localization of the Hippo pathway transcriptional regulator Yap1 and endocardial proliferation. Our work suggests that the growth of the endocardium results from myocardial chamber volume expansion and ends when the tension on the tissue is relaxed."}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["31511517"],"isi":["000485216800009"]},"isi":1,"quality_controlled":"1","doi":"10.1038/s41467-019-12068-x","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["20411723"]},"month":"09","pmid":1,"year":"2019","publisher":"Nature Publishing Group","department":[{"_id":"CaHe"}],"publication_status":"published","author":[{"first_name":"Dorothee","last_name":"Bornhorst","full_name":"Bornhorst, Dorothee"},{"first_name":"Peng","last_name":"Xia","id":"4AB6C7D0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5419-7756","full_name":"Xia, Peng"},{"last_name":"Nakajima","first_name":"Hiroyuki","full_name":"Nakajima, Hiroyuki"},{"full_name":"Dingare, Chaitanya","first_name":"Chaitanya","last_name":"Dingare"},{"last_name":"Herzog","first_name":"Wiebke","full_name":"Herzog, Wiebke"},{"first_name":"Virginie","last_name":"Lecaudey","full_name":"Lecaudey, Virginie"},{"full_name":"Mochizuki, Naoki","first_name":"Naoki","last_name":"Mochizuki"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"last_name":"Yelon","first_name":"Deborah","full_name":"Yelon, Deborah"},{"last_name":"Abdelilah-Seyfried","first_name":"Salim","full_name":"Abdelilah-Seyfried, Salim"}],"volume":10,"date_updated":"2023-08-30T06:21:23Z","date_created":"2019-09-22T22:00:37Z","file_date_updated":"2020-07-14T12:47:44Z"},{"publication":"The EMBO Journal","citation":{"short":"N. Petridou, C.-P.J. Heisenberg, The EMBO Journal 38 (2019).","mla":"Petridou, Nicoletta, and Carl-Philipp J. Heisenberg. “Tissue Rheology in Embryonic Organization.” The EMBO Journal, vol. 38, no. 20, e102497, EMBO, 2019, doi:10.15252/embj.2019102497.","chicago":"Petridou, Nicoletta, and Carl-Philipp J Heisenberg. “Tissue Rheology in Embryonic Organization.” The EMBO Journal. EMBO, 2019. https://doi.org/10.15252/embj.2019102497.","ama":"Petridou N, Heisenberg C-PJ. Tissue rheology in embryonic organization. The EMBO Journal. 2019;38(20). doi:10.15252/embj.2019102497","apa":"Petridou, N., & Heisenberg, C.-P. J. (2019). Tissue rheology in embryonic organization. The EMBO Journal. EMBO. https://doi.org/10.15252/embj.2019102497","ieee":"N. Petridou and C.-P. J. Heisenberg, “Tissue rheology in embryonic organization,” The EMBO Journal, vol. 38, no. 20. EMBO, 2019.","ista":"Petridou N, Heisenberg C-PJ. 2019. Tissue rheology in embryonic organization. The EMBO Journal. 38(20), e102497."},"article_type":"review","date_published":"2019-10-15T00:00:00Z","scopus_import":"1","day":"15","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"6980","status":"public","ddc":["570"],"title":"Tissue rheology in embryonic organization","intvolume":" 38","oa_version":"Published Version","file":[{"content_type":"application/pdf","file_size":847356,"creator":"dernst","file_name":"2019_Embo_Petridou.pdf","access_level":"open_access","date_updated":"2020-07-14T12:47:46Z","date_created":"2019-11-04T15:30:08Z","checksum":"76f7f4e79ab6d850c30017a69726fd85","relation":"main_file","file_id":"6981"}],"type":"journal_article","abstract":[{"text":"Tissue morphogenesis in multicellular organisms is brought about by spatiotemporal coordination of mechanical and chemical signals. Extensive work on how mechanical forces together with the well‐established morphogen signalling pathways can actively shape living tissues has revealed evolutionary conserved mechanochemical features of embryonic development. More recently, attention has been drawn to the description of tissue material properties and how they can influence certain morphogenetic processes. Interestingly, besides the role of tissue material properties in determining how much tissues deform in response to force application, there is increasing theoretical and experimental evidence, suggesting that tissue material properties can abruptly and drastically change in development. These changes resemble phase transitions, pointing at the intriguing possibility that important morphogenetic processes in development, such as symmetry breaking and self‐organization, might be mediated by tissue phase transitions. In this review, we summarize recent findings on the regulation and role of tissue material properties in the context of the developing embryo. We posit that abrupt changes of tissue rheological properties may have important implications in maintaining the balance between robustness and adaptability during embryonic development.","lang":"eng"}],"issue":"20","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["31512749"],"isi":["000485561900001"]},"oa":1,"quality_controlled":"1","isi":1,"project":[{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"},{"_id":"2693FD8C-B435-11E9-9278-68D0E5697425","grant_number":"V00736","call_identifier":"FWF","name":"Tissue material properties in embryonic development"}],"doi":"10.15252/embj.2019102497","language":[{"iso":"eng"}],"month":"10","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"year":"2019","pmid":1,"publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"EMBO","author":[{"full_name":"Petridou, Nicoletta","first_name":"Nicoletta","last_name":"Petridou","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8451-1195"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"date_updated":"2023-09-05T13:04:13Z","date_created":"2019-11-04T15:24:29Z","volume":38,"article_number":"e102497","file_date_updated":"2020-07-14T12:47:46Z","ec_funded":1},{"month":"02","publication_identifier":{"issn":["14657392"]},"acknowledged_ssus":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"doi":"10.1038/s41556-018-0247-4","isi":1,"quality_controlled":"1","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"grant_number":"ALTF710-2016","_id":"253E54C8-B435-11E9-9278-68D0E5697425","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants (EMBO fellowship)"}],"oa":1,"external_id":{"isi":["000457468300011"],"pmid":["30559456"]},"file_date_updated":"2020-10-21T07:18:35Z","ec_funded":1,"date_created":"2018-12-30T22:59:15Z","date_updated":"2023-09-11T14:03:28Z","volume":21,"author":[{"full_name":"Petridou, Nicoletta","orcid":"0000-0002-8451-1195","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","last_name":"Petridou","first_name":"Nicoletta"},{"last_name":"Grigolon","first_name":"Silvia","full_name":"Grigolon, Silvia"},{"last_name":"Salbreux","first_name":"Guillaume","full_name":"Salbreux, Guillaume"},{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"related_material":{"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/when-a-fish-becomes-fluid/"}]},"publication_status":"published","publisher":"Nature Publishing Group","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"year":"2019","pmid":1,"day":"01","has_accepted_license":"1","article_processing_charge":"No","scopus_import":"1","date_published":"2019-02-01T00:00:00Z","article_type":"original","page":"169–178","publication":"Nature Cell Biology","citation":{"mla":"Petridou, Nicoletta, et al. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” Nature Cell Biology, vol. 21, Nature Publishing Group, 2019, pp. 169–178, doi:10.1038/s41556-018-0247-4.","short":"N. Petridou, S. Grigolon, G. Salbreux, E.B. Hannezo, C.-P.J. Heisenberg, Nature Cell Biology 21 (2019) 169–178.","chicago":"Petridou, Nicoletta, Silvia Grigolon, Guillaume Salbreux, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Fluidization-Mediated Tissue Spreading by Mitotic Cell Rounding and Non-Canonical Wnt Signalling.” Nature Cell Biology. Nature Publishing Group, 2019. https://doi.org/10.1038/s41556-018-0247-4.","ama":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 2019;21:169–178. doi:10.1038/s41556-018-0247-4","ista":"Petridou N, Grigolon S, Salbreux G, Hannezo EB, Heisenberg C-PJ. 2019. Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. 21, 169–178.","ieee":"N. Petridou, S. Grigolon, G. Salbreux, E. B. Hannezo, and C.-P. J. Heisenberg, “Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling,” Nature Cell Biology, vol. 21. Nature Publishing Group, pp. 169–178, 2019.","apa":"Petridou, N., Grigolon, S., Salbreux, G., Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/s41556-018-0247-4"},"abstract":[{"lang":"eng","text":"Tissue morphogenesis is driven by mechanical forces that elicit changes in cell size, shape and motion. The extent by which forces deform tissues critically depends on the rheological properties of the recipient tissue. Yet, whether and how dynamic changes in tissue rheology affect tissue morphogenesis and how they are regulated within the developing organism remain unclear. Here, we show that blastoderm spreading at the onset of zebrafish morphogenesis relies on a rapid, pronounced and spatially patterned tissue fluidization. Blastoderm fluidization is temporally controlled by mitotic cell rounding-dependent cell–cell contact disassembly during the last rounds of cell cleavages. Moreover, fluidization is spatially restricted to the central blastoderm by local activation of non-canonical Wnt signalling within the blastoderm margin, increasing cell cohesion and thereby counteracting the effect of mitotic rounding on contact disassembly. Overall, our results identify a fluidity transition mediated by loss of cell cohesion as a critical regulator of embryo morphogenesis."}],"type":"journal_article","file":[{"file_id":"8685","relation":"main_file","date_created":"2020-10-21T07:18:35Z","date_updated":"2020-10-21T07:18:35Z","success":1,"checksum":"e38523787b3bc84006f2793de99ad70f","file_name":"2018_NatureCellBio_Petridou_accepted.pdf","access_level":"open_access","creator":"dernst","file_size":71590590,"content_type":"application/pdf"}],"oa_version":"Submitted Version","status":"public","title":"Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling","ddc":["570"],"intvolume":" 21","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"5789"},{"ec_funded":1,"file_date_updated":"2020-10-21T07:22:34Z","volume":177,"date_created":"2019-06-02T21:59:12Z","date_updated":"2024-03-28T23:30:39Z","related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/how-the-cytoplasm-separates-from-the-yolk/"}],"record":[{"id":"8350","relation":"dissertation_contains","status":"public"}]},"author":[{"full_name":"Shamipour, Shayan","last_name":"Shamipour","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kardos, Roland","last_name":"Kardos","first_name":"Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Xue, Shi-lei","first_name":"Shi-lei","last_name":"Xue","id":"31D2C804-F248-11E8-B48F-1D18A9856A87"},{"id":"3A374330-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2057-2754","first_name":"Björn","last_name":"Hof","full_name":"Hof, Björn"},{"full_name":"Hannezo, Edouard B","first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"publisher":"Elsevier","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"BjHo"}],"publication_status":"published","pmid":1,"year":"2019","acknowledgement":"We would like to thank Pierre Recho, Guillaume Salbreux, and Silvia Grigolon for advice on the theory, Lila Solnica-Krezel for kindly providing us with zebrafish dachsous mutants, members of the Heisenberg and Hannezo groups for fruitful discussions, and the Bioimaging and zebrafish facilities at IST Austria for their continuous support. This project has received funding from the European Union (European Research Council Advanced Grant 742573 to C.P.H.) and from the Austrian Science Fund (FWF) (P 31639 to E.H.).","publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"month":"05","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"doi":"10.1016/j.cell.2019.04.030","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"_id":"268294B6-B435-11E9-9278-68D0E5697425","grant_number":"P31639","call_identifier":"FWF","name":"Active mechano-chemical description of the cell cytoskeleton"}],"quality_controlled":"1","isi":1,"external_id":{"pmid":["31080065"],"isi":["000469415100013"]},"oa":1,"main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2019.04.030","open_access":"1"}],"issue":"6","abstract":[{"text":"Segregation of maternal determinants within the oocyte constitutes the first step in embryo patterning. In zebrafish oocytes, extensive ooplasmic streaming leads to the segregation of ooplasm from yolk granules along the animal-vegetal axis of the oocyte. Here, we show that this process does not rely on cortical actin reorganization, as previously thought, but instead on a cell-cycle-dependent bulk actin polymerization wave traveling from the animal to the vegetal pole of the oocyte. This wave functions in segregation by both pulling ooplasm animally and pushing yolk granules vegetally. Using biophysical experimentation and theory, we show that ooplasm pulling is mediated by bulk actin network flows exerting friction forces on the ooplasm, while yolk granule pushing is achieved by a mechanism closely resembling actin comet formation on yolk granules. Our study defines a novel role of cell-cycle-controlled bulk actin polymerization waves in oocyte polarization via ooplasmic segregation.","lang":"eng"}],"type":"journal_article","file":[{"file_id":"8686","relation":"main_file","date_created":"2020-10-21T07:22:34Z","date_updated":"2020-10-21T07:22:34Z","success":1,"checksum":"aea43726d80e35ce3885073a5f05c3e3","file_name":"2019_Cell_Shamipour_accepted.pdf","access_level":"open_access","creator":"dernst","content_type":"application/pdf","file_size":3356292}],"oa_version":"Published Version","intvolume":" 177","title":"Bulk actin dynamics drive phase segregation in zebrafish oocytes","status":"public","ddc":["570"],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6508","has_accepted_license":"1","article_processing_charge":"No","day":"30","scopus_import":"1","date_published":"2019-05-30T00:00:00Z","page":"1463-1479.e18","article_type":"original","citation":{"mla":"Shamipour, Shayan, et al. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” Cell, vol. 177, no. 6, Elsevier, 2019, p. 1463–1479.e18, doi:10.1016/j.cell.2019.04.030.","short":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E.B. Hannezo, C.-P.J. Heisenberg, Cell 177 (2019) 1463–1479.e18.","chicago":"Shamipour, Shayan, Roland Kardos, Shi-lei Xue, Björn Hof, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.030.","ama":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. 2019;177(6):1463-1479.e18. doi:10.1016/j.cell.2019.04.030","ista":"Shamipour S, Kardos R, Xue S, Hof B, Hannezo EB, Heisenberg C-PJ. 2019. Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. 177(6), 1463–1479.e18.","apa":"Shamipour, S., Kardos, R., Xue, S., Hof, B., Hannezo, E. B., & Heisenberg, C.-P. J. (2019). Bulk actin dynamics drive phase segregation in zebrafish oocytes. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.030","ieee":"S. Shamipour, R. Kardos, S. Xue, B. Hof, E. B. Hannezo, and C.-P. J. Heisenberg, “Bulk actin dynamics drive phase segregation in zebrafish oocytes,” Cell, vol. 177, no. 6. Elsevier, p. 1463–1479.e18, 2019."},"publication":"Cell"},{"type":"journal_article","issue":"4","title":"Mechanosensation of tight junctions depends on ZO-1 phase separation and flow","status":"public","ddc":["570"],"intvolume":" 179","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"7001","oa_version":"Submitted Version","file":[{"file_id":"8684","relation":"main_file","date_updated":"2020-10-21T07:09:45Z","date_created":"2020-10-21T07:09:45Z","success":1,"checksum":"33dac4bb77ee630e2666e936b4d57980","file_name":"2019_Cell_Schwayer_accepted.pdf","access_level":"open_access","creator":"dernst","file_size":8805878,"content_type":"application/pdf"}],"scopus_import":"1","day":"31","article_processing_charge":"No","has_accepted_license":"1","article_type":"original","page":"937-952.e18","publication":"Cell","citation":{"chicago":"Schwayer, Cornelia, Shayan Shamipour, Kornelija Pranjic-Ferscha, Alexandra Schauer, M Balda, M Tada, K Matter, and Carl-Philipp J Heisenberg. “Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow.” Cell. Cell Press, 2019. https://doi.org/10.1016/j.cell.2019.10.006.","mla":"Schwayer, Cornelia, et al. “Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow.” Cell, vol. 179, no. 4, Cell Press, 2019, p. 937–952.e18, doi:10.1016/j.cell.2019.10.006.","short":"C. Schwayer, S. Shamipour, K. Pranjic-Ferscha, A. Schauer, M. Balda, M. Tada, K. Matter, C.-P.J. Heisenberg, Cell 179 (2019) 937–952.e18.","ista":"Schwayer C, Shamipour S, Pranjic-Ferscha K, Schauer A, Balda M, Tada M, Matter K, Heisenberg C-PJ. 2019. Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Cell. 179(4), 937–952.e18.","apa":"Schwayer, C., Shamipour, S., Pranjic-Ferscha, K., Schauer, A., Balda, M., Tada, M., … Heisenberg, C.-P. J. (2019). Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Cell. Cell Press. https://doi.org/10.1016/j.cell.2019.10.006","ieee":"C. Schwayer et al., “Mechanosensation of tight junctions depends on ZO-1 phase separation and flow,” Cell, vol. 179, no. 4. Cell Press, p. 937–952.e18, 2019.","ama":"Schwayer C, Shamipour S, Pranjic-Ferscha K, et al. Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Cell. 2019;179(4):937-952.e18. doi:10.1016/j.cell.2019.10.006"},"date_published":"2019-10-31T00:00:00Z","file_date_updated":"2020-10-21T07:09:45Z","ec_funded":1,"publication_status":"published","publisher":"Cell Press","department":[{"_id":"CaHe"},{"_id":"BjHo"}],"year":"2019","pmid":1,"date_updated":"2024-03-28T23:30:39Z","date_created":"2019-11-12T12:51:06Z","volume":179,"author":[{"first_name":"Cornelia","last_name":"Schwayer","id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226","full_name":"Schwayer, Cornelia"},{"last_name":"Shamipour","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan"},{"full_name":"Pranjic-Ferscha, Kornelija","first_name":"Kornelija","last_name":"Pranjic-Ferscha","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Schauer, Alexandra","orcid":"0000-0001-7659-9142","id":"30A536BA-F248-11E8-B48F-1D18A9856A87","last_name":"Schauer","first_name":"Alexandra"},{"full_name":"Balda, M","first_name":"M","last_name":"Balda"},{"last_name":"Tada","first_name":"M","full_name":"Tada, M"},{"full_name":"Matter, K","first_name":"K","last_name":"Matter"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"7186"},{"status":"public","relation":"dissertation_contains","id":"8350"}],"link":[{"description":"News auf IST Website","relation":"press_release","url":"https://ist.ac.at/en/news/biochemistry-meets-mechanics-the-sensitive-nature-of-cell-cell-contact-formation-in-embryo-development/"}]},"month":"10","publication_identifier":{"eissn":["1097-4172"],"issn":["0092-8674"]},"isi":1,"quality_controlled":"1","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"}],"oa":1,"external_id":{"isi":["000493898000012"],"pmid":["31675500"]},"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"doi":"10.1016/j.cell.2019.10.006"},{"publication":"Journal of Cell Biology","citation":{"ieee":"L. Carvalho et al., “Occluding junctions as novel regulators of tissue mechanics during wound repair,” Journal of Cell Biology, vol. 217, no. 12. Rockefeller University Press, pp. 4267–4283, 2018.","apa":"Carvalho, L., Patricio, P., Ponte, S., Heisenberg, C.-P. J., Almeida, L., Nunes, A. S., … Jacinto, A. (2018). Occluding junctions as novel regulators of tissue mechanics during wound repair. Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.201804048","ista":"Carvalho L, Patricio P, Ponte S, Heisenberg C-PJ, Almeida L, Nunes AS, Araújo NAM, Jacinto A. 2018. Occluding junctions as novel regulators of tissue mechanics during wound repair. Journal of Cell Biology. 217(12), 4267–4283.","ama":"Carvalho L, Patricio P, Ponte S, et al. Occluding junctions as novel regulators of tissue mechanics during wound repair. Journal of Cell Biology. 2018;217(12):4267-4283. doi:10.1083/jcb.201804048","chicago":"Carvalho, Lara, Pedro Patricio, Susana Ponte, Carl-Philipp J Heisenberg, Luis Almeida, André S. Nunes, Nuno A.M. Araújo, and Antonio Jacinto. “Occluding Junctions as Novel Regulators of Tissue Mechanics during Wound Repair.” Journal of Cell Biology. Rockefeller University Press, 2018. https://doi.org/10.1083/jcb.201804048.","short":"L. Carvalho, P. Patricio, S. Ponte, C.-P.J. Heisenberg, L. Almeida, A.S. Nunes, N.A.M. Araújo, A. Jacinto, Journal of Cell Biology 217 (2018) 4267–4283.","mla":"Carvalho, Lara, et al. “Occluding Junctions as Novel Regulators of Tissue Mechanics during Wound Repair.” Journal of Cell Biology, vol. 217, no. 12, Rockefeller University Press, 2018, pp. 4267–83, doi:10.1083/jcb.201804048."},"page":"4267-4283","date_published":"2018-12-01T00:00:00Z","scopus_import":"1","day":"01","article_processing_charge":"No","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"5676","status":"public","title":"Occluding junctions as novel regulators of tissue mechanics during wound repair","intvolume":" 217","oa_version":"Submitted Version","type":"journal_article","abstract":[{"text":"In epithelial tissues, cells tightly connect to each other through cell–cell junctions, but they also present the remarkable capacity of reorganizing themselves without compromising tissue integrity. Upon injury, simple epithelia efficiently resolve small lesions through the action of actin cytoskeleton contractile structures at the wound edge and cellular rearrangements. However, the underlying mechanisms and how they cooperate are still poorly understood. In this study, we combine live imaging and theoretical modeling to reveal a novel and indispensable role for occluding junctions (OJs) in this process. We demonstrate that OJ loss of function leads to defects in wound-closure dynamics: instead of contracting, wounds dramatically increase their area. OJ mutants exhibit phenotypes in cell shape, cellular rearrangements, and mechanical properties as well as in actin cytoskeleton dynamics at the wound edge. We propose that OJs are essential for wound closure by impacting on epithelial mechanics at the tissue level, which in turn is crucial for correct regulation of the cellular events occurring at the wound edge.","lang":"eng"}],"issue":"12","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pubmed/30228162"}],"oa":1,"external_id":{"pmid":["30228162 "],"isi":["000451960800018"]},"quality_controlled":"1","isi":1,"project":[{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"}],"doi":"10.1083/jcb.201804048","language":[{"iso":"eng"}],"month":"12","publication_identifier":{"issn":["00219525"]},"year":"2018","pmid":1,"publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Rockefeller University Press","author":[{"full_name":"Carvalho, Lara","first_name":"Lara","last_name":"Carvalho"},{"first_name":"Pedro","last_name":"Patricio","full_name":"Patricio, Pedro"},{"first_name":"Susana","last_name":"Ponte","full_name":"Ponte, Susana"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"full_name":"Almeida, Luis","last_name":"Almeida","first_name":"Luis"},{"full_name":"Nunes, André S.","last_name":"Nunes","first_name":"André S."},{"last_name":"Araújo","first_name":"Nuno A.M.","full_name":"Araújo, Nuno A.M."},{"full_name":"Jacinto, Antonio","first_name":"Antonio","last_name":"Jacinto"}],"date_updated":"2023-09-13T09:11:17Z","date_created":"2018-12-16T22:59:19Z","volume":217,"ec_funded":1},{"scopus_import":1,"month":"05","day":"31","publication_identifier":{"issn":["14657392"]},"publication":"Nature Cell Biology","citation":{"ama":"Petridou N, Spiro ZP, Heisenberg C-PJ. Multiscale force sensing in development. Nature Cell Biology. 2017;19(6):581-588. doi:10.1038/ncb3524","apa":"Petridou, N., Spiro, Z. P., & Heisenberg, C.-P. J. (2017). Multiscale force sensing in development. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb3524","ieee":"N. Petridou, Z. P. Spiro, and C.-P. J. Heisenberg, “Multiscale force sensing in development,” Nature Cell Biology, vol. 19, no. 6. Nature Publishing Group, pp. 581–588, 2017.","ista":"Petridou N, Spiro ZP, Heisenberg C-PJ. 2017. Multiscale force sensing in development. Nature Cell Biology. 19(6), 581–588.","short":"N. Petridou, Z.P. Spiro, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 581–588.","mla":"Petridou, Nicoletta, et al. “Multiscale Force Sensing in Development.” Nature Cell Biology, vol. 19, no. 6, Nature Publishing Group, 2017, pp. 581–88, doi:10.1038/ncb3524.","chicago":"Petridou, Nicoletta, Zoltan P Spiro, and Carl-Philipp J Heisenberg. “Multiscale Force Sensing in Development.” Nature Cell Biology. Nature Publishing Group, 2017. https://doi.org/10.1038/ncb3524."},"quality_controlled":"1","project":[{"name":"The generation and function of anisotropic tissue tension in zebrafish epiboly (EMBO Fellowship)","_id":"25236028-B435-11E9-9278-68D0E5697425","grant_number":"ALTF534-2016"}],"page":"581 - 588","doi":"10.1038/ncb3524","date_published":"2017-05-31T00:00:00Z","language":[{"iso":"eng"}],"type":"journal_article","abstract":[{"lang":"eng","text":"The seminal observation that mechanical signals can elicit changes in biochemical signalling within cells, a process commonly termed mechanosensation and mechanotransduction, has revolutionized our understanding of the role of cell mechanics in various fundamental biological processes, such as cell motility, adhesion, proliferation and differentiation. In this Review, we will discuss how the interplay and feedback between mechanical and biochemical signals control tissue morphogenesis and cell fate specification in embryonic development."}],"publist_id":"7040","issue":"6","_id":"678","year":"2017","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publication_status":"published","title":"Multiscale force sensing in development","status":"public","department":[{"_id":"CaHe"}],"intvolume":" 19","publisher":"Nature Publishing Group","author":[{"full_name":"Petridou, Nicoletta","last_name":"Petridou","first_name":"Nicoletta","orcid":"0000-0002-8451-1195","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87"},{"id":"426AD026-F248-11E8-B48F-1D18A9856A87","last_name":"Spiro","first_name":"Zoltan P","full_name":"Spiro, Zoltan P"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"date_created":"2018-12-11T11:47:53Z","date_updated":"2021-01-12T08:08:59Z","volume":19,"oa_version":"None"},{"publication_identifier":{"issn":["09254773"]},"day":"01","month":"06","scopus_import":1,"doi":"10.1016/j.mod.2017.03.006","date_published":"2017-06-01T00:00:00Z","language":[{"iso":"eng"}],"citation":{"ama":"Heisenberg C-PJ. D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. Mechanisms of Development. 2017;145:32-37. doi:10.1016/j.mod.2017.03.006","ista":"Heisenberg C-PJ. 2017. D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. Mechanisms of Development. 145, 32–37.","apa":"Heisenberg, C.-P. J. (2017). D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. Mechanisms of Development. Elsevier. https://doi.org/10.1016/j.mod.2017.03.006","ieee":"C.-P. J. Heisenberg, “D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization,” Mechanisms of Development, vol. 145. Elsevier, pp. 32–37, 2017.","mla":"Heisenberg, Carl-Philipp J. “D’Arcy Thompson’s ‘on Growth and Form’: From Soap Bubbles to Tissue Self Organization.” Mechanisms of Development, vol. 145, Elsevier, 2017, pp. 32–37, doi:10.1016/j.mod.2017.03.006.","short":"C.-P.J. Heisenberg, Mechanisms of Development 145 (2017) 32–37.","chicago":"Heisenberg, Carl-Philipp J. “D’Arcy Thompson’s ‘on Growth and Form’: From Soap Bubbles to Tissue Self Organization.” Mechanisms of Development. Elsevier, 2017. https://doi.org/10.1016/j.mod.2017.03.006."},"publication":"Mechanisms of Development","page":"32 - 37","quality_controlled":"1","publist_id":"7024","abstract":[{"lang":"eng","text":"Tissues are thought to behave like fluids with a given surface tension. Differences in tissue surface tension (TST) have been proposed to trigger cell sorting and tissue envelopment. D'Arcy Thompson in his seminal book ‘On Growth and Form’ has introduced this concept of differential TST as a key physical mechanism dictating tissue formation and organization within the developing organism. Over the past century, many studies have picked up the concept of differential TST and analyzed the role and cell biological basis of TST in development, underlining the importance and influence of this concept in developmental biology."}],"type":"journal_article","author":[{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"oa_version":"None","volume":145,"date_created":"2018-12-11T11:47:55Z","date_updated":"2021-01-12T08:09:23Z","year":"2017","_id":"686","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Elsevier","intvolume":" 145","department":[{"_id":"CaHe"}],"title":"D'Arcy Thompson's ‘on growth and form’: From soap bubbles to tissue self organization","status":"public","publication_status":"published"},{"month":"02","publication_identifier":{"issn":["15345807"]},"acknowledged_ssus":[{"_id":"PreCl"}],"language":[{"iso":"eng"}],"doi":"10.1016/j.devcel.2017.01.010","quality_controlled":"1","isi":1,"project":[{"grant_number":"201439","_id":"2524F500-B435-11E9-9278-68D0E5697425","name":"Developing High-Throughput Bioassays for Human Cancers in Zebrafish","call_identifier":"FP7"}],"external_id":{"isi":["000395368300007"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"file_date_updated":"2018-12-12T10:10:57Z","publist_id":"6320","ec_funded":1,"date_created":"2018-12-11T11:49:58Z","date_updated":"2023-09-20T12:06:27Z","volume":40,"author":[{"last_name":"Morita","first_name":"Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","full_name":"Morita, Hitoshi"},{"first_name":"Silvia","last_name":"Grigolon","full_name":"Grigolon, Silvia"},{"full_name":"Bock, Martin","first_name":"Martin","last_name":"Bock"},{"full_name":"Krens, Gabriel","last_name":"Krens","first_name":"Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Guillaume","last_name":"Salbreux","full_name":"Salbreux, Guillaume"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Cell Press","year":"2017","day":"27","article_processing_charge":"No","has_accepted_license":"1","scopus_import":"1","date_published":"2017-02-27T00:00:00Z","page":"354 - 366","publication":"Developmental Cell","citation":{"short":"H. Morita, S. Grigolon, M. Bock, G. Krens, G. Salbreux, C.-P.J. Heisenberg, Developmental Cell 40 (2017) 354–366.","mla":"Morita, Hitoshi, et al. “The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.” Developmental Cell, vol. 40, no. 4, Cell Press, 2017, pp. 354–66, doi:10.1016/j.devcel.2017.01.010.","chicago":"Morita, Hitoshi, Silvia Grigolon, Martin Bock, Gabriel Krens, Guillaume Salbreux, and Carl-Philipp J Heisenberg. “The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.” Developmental Cell. Cell Press, 2017. https://doi.org/10.1016/j.devcel.2017.01.010.","ama":"Morita H, Grigolon S, Bock M, Krens G, Salbreux G, Heisenberg C-PJ. The physical basis of coordinated tissue spreading in zebrafish gastrulation. Developmental Cell. 2017;40(4):354-366. doi:10.1016/j.devcel.2017.01.010","apa":"Morita, H., Grigolon, S., Bock, M., Krens, G., Salbreux, G., & Heisenberg, C.-P. J. (2017). The physical basis of coordinated tissue spreading in zebrafish gastrulation. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2017.01.010","ieee":"H. Morita, S. Grigolon, M. Bock, G. Krens, G. Salbreux, and C.-P. J. Heisenberg, “The physical basis of coordinated tissue spreading in zebrafish gastrulation,” Developmental Cell, vol. 40, no. 4. Cell Press, pp. 354–366, 2017.","ista":"Morita H, Grigolon S, Bock M, Krens G, Salbreux G, Heisenberg C-PJ. 2017. The physical basis of coordinated tissue spreading in zebrafish gastrulation. Developmental Cell. 40(4), 354–366."},"abstract":[{"lang":"eng","text":"Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their movements to shape the embryo. In zebrafish embryogenesis, coordinated tissue movements first become apparent during “doming,” when the blastoderm begins to spread over the yolk sac, a process involving coordinated epithelial surface cell layer expansion and mesenchymal deep cell intercalations. Here, we find that active surface cell expansion represents the key process coordinating tissue movements during doming. By using a combination of theory and experiments, we show that epithelial surface cells not only trigger blastoderm expansion by reducing tissue surface tension, but also drive blastoderm thinning by inducing tissue contraction through radial deep cell intercalations. Thus, coordinated tissue expansion and thinning during doming relies on surface cells simultaneously controlling tissue surface tension and radial tissue contraction."}],"issue":"4","type":"journal_article","oa_version":"Published Version","file":[{"file_size":6866187,"content_type":"application/pdf","creator":"system","file_name":"IST-2017-869-v1+1_1-s2.0-S1534580717300370-main.pdf","access_level":"open_access","date_created":"2018-12-12T10:10:57Z","date_updated":"2018-12-12T10:10:57Z","relation":"main_file","file_id":"4849"}],"pubrep_id":"869","ddc":["572","597"],"status":"public","title":"The physical basis of coordinated tissue spreading in zebrafish gastrulation","intvolume":" 40","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"1067"},{"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"1025","intvolume":" 543","title":"Cell biology: Stretched divisions","status":"public","oa_version":"None","type":"journal_article","issue":"7643","abstract":[{"lang":"eng","text":"Many organ surfaces are covered by a protective epithelial-cell layer. It emerges that such layers are maintained by cell stretching that triggers cell division mediated by the force-sensitive ion-channel protein Piezo1. See Letter p.118"}],"citation":{"short":"C.-P.J. Heisenberg, Nature 543 (2017) 43–44.","mla":"Heisenberg, Carl-Philipp J. “Cell Biology: Stretched Divisions.” Nature, vol. 543, no. 7643, Nature Publishing Group, 2017, pp. 43–44, doi:10.1038/nature21502.","chicago":"Heisenberg, Carl-Philipp J. “Cell Biology: Stretched Divisions.” Nature. Nature Publishing Group, 2017. https://doi.org/10.1038/nature21502.","ama":"Heisenberg C-PJ. Cell biology: Stretched divisions. Nature. 2017;543(7643):43-44. doi:10.1038/nature21502","apa":"Heisenberg, C.-P. J. (2017). Cell biology: Stretched divisions. Nature. Nature Publishing Group. https://doi.org/10.1038/nature21502","ieee":"C.-P. J. Heisenberg, “Cell biology: Stretched divisions,” Nature, vol. 543, no. 7643. Nature Publishing Group, pp. 43–44, 2017.","ista":"Heisenberg C-PJ. 2017. Cell biology: Stretched divisions. Nature. 543(7643), 43–44."},"publication":"Nature","page":"43 - 44","date_published":"2017-03-02T00:00:00Z","scopus_import":"1","article_processing_charge":"No","day":"02","year":"2017","department":[{"_id":"CaHe"}],"publisher":"Nature Publishing Group","publication_status":"published","author":[{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"volume":543,"date_updated":"2023-09-22T09:26:59Z","date_created":"2018-12-11T11:49:45Z","publist_id":"6367","external_id":{"isi":["000395671500025"]},"isi":1,"quality_controlled":"1","doi":"10.1038/nature21502","language":[{"iso":"eng"}],"publication_identifier":{"issn":["00280836"]},"month":"03"},{"oa_version":"Submitted Version","_id":"804","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","title":"Overcoming the limitations of the MARTINI force field in simulations of polysaccharides","intvolume":" 13","abstract":[{"text":"Polysaccharides (carbohydrates) are key regulators of a large number of cell biological processes. However, precise biochemical or genetic manipulation of these often complex structures is laborious and hampers experimental structure–function studies. Molecular Dynamics (MD) simulations provide a valuable alternative tool to generate and test hypotheses on saccharide function. Yet, currently used MD force fields often overestimate the aggregation propensity of polysaccharides, affecting the usability of those simulations. Here we tested MARTINI, a popular coarse-grained (CG) force field for biological macromolecules, for its ability to accurately represent molecular forces between saccharides. To this end, we calculated a thermodynamic solution property, the second virial coefficient of the osmotic pressure (B22). Comparison with light scattering experiments revealed a nonphysical aggregation of a prototypical polysaccharide in MARTINI, pointing at an imbalance of the nonbonded solute–solute, solute–water, and water–water interactions. This finding also applies to smaller oligosaccharides which were all found to aggregate in simulations even at moderate concentrations, well below their solubility limit. Finally, we explored the influence of the Lennard-Jones (LJ) interaction between saccharide molecules and propose a simple scaling of the LJ interaction strength that makes MARTINI more reliable for the simulation of saccharides.","lang":"eng"}],"issue":"10","type":"journal_article","date_published":"2017-10-10T00:00:00Z","publication":"Journal of Chemical Theory and Computation","citation":{"short":"P.S. Schmalhorst, F. Deluweit, R. Scherrers, C.-P.J. Heisenberg, M.K. Sikora, Journal of Chemical Theory and Computation 13 (2017) 5039–5053.","mla":"Schmalhorst, Philipp S., et al. “Overcoming the Limitations of the MARTINI Force Field in Simulations of Polysaccharides.” Journal of Chemical Theory and Computation, vol. 13, no. 10, American Chemical Society, 2017, pp. 5039–53, doi:10.1021/acs.jctc.7b00374.","chicago":"Schmalhorst, Philipp S, Felix Deluweit, Roger Scherrers, Carl-Philipp J Heisenberg, and Mateusz K Sikora. “Overcoming the Limitations of the MARTINI Force Field in Simulations of Polysaccharides.” Journal of Chemical Theory and Computation. American Chemical Society, 2017. https://doi.org/10.1021/acs.jctc.7b00374.","ama":"Schmalhorst PS, Deluweit F, Scherrers R, Heisenberg C-PJ, Sikora MK. Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. Journal of Chemical Theory and Computation. 2017;13(10):5039-5053. doi:10.1021/acs.jctc.7b00374","apa":"Schmalhorst, P. S., Deluweit, F., Scherrers, R., Heisenberg, C.-P. J., & Sikora, M. K. (2017). Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. Journal of Chemical Theory and Computation. American Chemical Society. https://doi.org/10.1021/acs.jctc.7b00374","ieee":"P. S. Schmalhorst, F. Deluweit, R. Scherrers, C.-P. J. Heisenberg, and M. K. Sikora, “Overcoming the limitations of the MARTINI force field in simulations of polysaccharides,” Journal of Chemical Theory and Computation, vol. 13, no. 10. American Chemical Society, pp. 5039–5053, 2017.","ista":"Schmalhorst PS, Deluweit F, Scherrers R, Heisenberg C-PJ, Sikora MK. 2017. Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. Journal of Chemical Theory and Computation. 13(10), 5039–5053."},"page":"5039 - 5053","day":"10","article_processing_charge":"No","scopus_import":"1","author":[{"full_name":"Schmalhorst, Philipp S","id":"309D50DA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5795-0133","first_name":"Philipp S","last_name":"Schmalhorst"},{"full_name":"Deluweit, Felix","first_name":"Felix","last_name":"Deluweit"},{"first_name":"Roger","last_name":"Scherrers","full_name":"Scherrers, Roger"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sikora, Mateusz K","first_name":"Mateusz K","last_name":"Sikora","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"}],"date_updated":"2023-09-27T10:58:45Z","date_created":"2018-12-11T11:48:35Z","volume":13,"acknowledgement":"P.S.S. was supported by research fellowship 2811/1-1 from the German Research Foundation (DFG), and M.S. was supported by EMBO Long Term Fellowship ALTF 187-2013 and Grant GC65-32 from the Interdisciplinary Centre for Mathematical and Computational Modelling (ICM), University of Warsaw, Poland. The authors thank Antje Potthast, Marek Cieplak, Tomasz Włodarski, and Damien Thompson for fruitful discussions and the IST Austria Scientific Computing Facility for support.","year":"2017","publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"American Chemical Society","publist_id":"6847","doi":"10.1021/acs.jctc.7b00374","acknowledged_ssus":[{"_id":"ScienComp"}],"language":[{"iso":"eng"}],"main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1704.03773"}],"external_id":{"isi":["000412965700036"]},"oa":1,"isi":1,"quality_controlled":"1","month":"10","publication_identifier":{"issn":["15499618"]}},{"publist_id":"6949","author":[{"full_name":"Chan, Chii","last_name":"Chan","first_name":"Chii"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"last_name":"Hiiragi","first_name":"Takashi","full_name":"Hiiragi, Takashi"}],"date_created":"2018-12-11T11:48:11Z","date_updated":"2023-09-28T11:33:21Z","volume":27,"year":"2017","publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Cell Press","month":"09","publication_identifier":{"issn":["09609822"]},"doi":"10.1016/j.cub.2017.07.010","language":[{"iso":"eng"}],"external_id":{"isi":["000411581800019"]},"isi":1,"quality_controlled":"1","abstract":[{"lang":"eng","text":"During animal development, cell-fate-specific changes in gene expression can modify the material properties of a tissue and drive tissue morphogenesis. While mechanistic insights into the genetic control of tissue-shaping events are beginning to emerge, how tissue morphogenesis and mechanics can reciprocally impact cell-fate specification remains relatively unexplored. Here we review recent findings reporting how multicellular morphogenetic events and their underlying mechanical forces can feed back into gene regulatory pathways to specify cell fate. We further discuss emerging techniques that allow for the direct measurement and manipulation of mechanical signals in vivo, offering unprecedented access to study mechanotransduction during development. Examination of the mechanical control of cell fate during tissue morphogenesis will pave the way to an integrated understanding of the design principles that underlie robust tissue patterning in embryonic development."}],"issue":"18","type":"journal_article","oa_version":"None","_id":"728","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Coordination of morphogenesis and cell fate specification in development","status":"public","intvolume":" 27","day":"18","article_processing_charge":"No","scopus_import":"1","date_published":"2017-09-18T00:00:00Z","publication":"Current Biology","citation":{"chicago":"Chan, Chii, Carl-Philipp J Heisenberg, and Takashi Hiiragi. “Coordination of Morphogenesis and Cell Fate Specification in Development.” Current Biology. Cell Press, 2017. https://doi.org/10.1016/j.cub.2017.07.010.","mla":"Chan, Chii, et al. “Coordination of Morphogenesis and Cell Fate Specification in Development.” Current Biology, vol. 27, no. 18, Cell Press, 2017, pp. R1024–35, doi:10.1016/j.cub.2017.07.010.","short":"C. Chan, C.-P.J. Heisenberg, T. Hiiragi, Current Biology 27 (2017) R1024–R1035.","ista":"Chan C, Heisenberg C-PJ, Hiiragi T. 2017. Coordination of morphogenesis and cell fate specification in development. Current Biology. 27(18), R1024–R1035.","apa":"Chan, C., Heisenberg, C.-P. J., & Hiiragi, T. (2017). Coordination of morphogenesis and cell fate specification in development. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2017.07.010","ieee":"C. Chan, C.-P. J. Heisenberg, and T. Hiiragi, “Coordination of morphogenesis and cell fate specification in development,” Current Biology, vol. 27, no. 18. Cell Press, pp. R1024–R1035, 2017.","ama":"Chan C, Heisenberg C-PJ, Hiiragi T. Coordination of morphogenesis and cell fate specification in development. Current Biology. 2017;27(18):R1024-R1035. doi:10.1016/j.cub.2017.07.010"},"page":"R1024 - R1035"},{"article_processing_charge":"No","day":"01","scopus_import":"1","date_published":"2017-01-01T00:00:00Z","page":"559 - 560","citation":{"mla":"Spiro, Zoltan P., and Carl-Philipp J. Heisenberg. “Regeneration Tensed up Polyploidy Takes the Lead.” Developmental Cell, vol. 42, no. 6, Cell Press, 2017, pp. 559–60, doi:10.1016/j.devcel.2017.09.008.","short":"Z.P. Spiro, C.-P.J. Heisenberg, Developmental Cell 42 (2017) 559–560.","chicago":"Spiro, Zoltan P, and Carl-Philipp J Heisenberg. “Regeneration Tensed up Polyploidy Takes the Lead.” Developmental Cell. Cell Press, 2017. https://doi.org/10.1016/j.devcel.2017.09.008.","ama":"Spiro ZP, Heisenberg C-PJ. Regeneration tensed up polyploidy takes the lead. Developmental Cell. 2017;42(6):559-560. doi:10.1016/j.devcel.2017.09.008","ista":"Spiro ZP, Heisenberg C-PJ. 2017. Regeneration tensed up polyploidy takes the lead. Developmental Cell. 42(6), 559–560.","ieee":"Z. P. Spiro and C.-P. J. Heisenberg, “Regeneration tensed up polyploidy takes the lead,” Developmental Cell, vol. 42, no. 6. Cell Press, pp. 559–560, 2017.","apa":"Spiro, Z. P., & Heisenberg, C.-P. J. (2017). Regeneration tensed up polyploidy takes the lead. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2017.09.008"},"publication":"Developmental Cell","issue":"6","abstract":[{"lang":"eng","text":"The cellular mechanisms allowing tissues to efficiently regenerate are not fully understood. In this issue of Developmental Cell, Cao et al. (2017)) discover that during zebrafish heart regeneration, epicardial cells at the leading edge of regenerating tissue undergo endoreplication, possibly due to increased tissue tension, thereby boosting their regenerative capacity."}],"type":"journal_article","oa_version":"None","intvolume":" 42","status":"public","title":"Regeneration tensed up polyploidy takes the lead","_id":"729","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_identifier":{"issn":["15345807"]},"month":"01","language":[{"iso":"eng"}],"doi":"10.1016/j.devcel.2017.09.008","isi":1,"quality_controlled":"1","external_id":{"isi":["000411582800003"]},"publist_id":"6948","volume":42,"date_created":"2018-12-11T11:48:11Z","date_updated":"2023-09-28T11:32:49Z","author":[{"first_name":"Zoltan P","last_name":"Spiro","id":"426AD026-F248-11E8-B48F-1D18A9856A87","full_name":"Spiro, Zoltan P"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"department":[{"_id":"CaHe"}],"publisher":"Cell Press","publication_status":"published","year":"2017"},{"date_updated":"2024-03-28T23:30:26Z","date_created":"2018-12-11T11:47:52Z","volume":144,"author":[{"full_name":"Krens, Gabriel","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996"},{"full_name":"Veldhuis, Jim","first_name":"Jim","last_name":"Veldhuis"},{"full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone","first_name":"Vanessa"},{"full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940","id":"31C42484-F248-11E8-B48F-1D18A9856A87","last_name":"Capek","first_name":"Daniel"},{"full_name":"Maître, Jean-Léon","first_name":"Jean-Léon","last_name":"Maître","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474"},{"last_name":"Brodland","first_name":"Wayne","full_name":"Brodland, Wayne"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"961"},{"status":"public","relation":"dissertation_contains","id":"50"}]},"publication_status":"published","department":[{"_id":"Bio"},{"_id":"CaHe"}],"publisher":"Company of Biologists","year":"2017","pmid":1,"file_date_updated":"2020-07-14T12:47:39Z","publist_id":"7047","language":[{"iso":"eng"}],"doi":"10.1242/dev.144964","quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["28512197"]},"oa":1,"month":"05","publication_identifier":{"issn":["09501991"]},"file":[{"checksum":"bc25125fb664706cdf180e061429f91d","date_created":"2019-09-24T06:56:22Z","date_updated":"2020-07-14T12:47:39Z","relation":"main_file","file_id":"6905","content_type":"application/pdf","file_size":8194516,"creator":"dernst","access_level":"open_access","file_name":"2017_Development_Krens.pdf"}],"oa_version":"Published Version","ddc":["570"],"status":"public","title":"Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation","intvolume":" 144","_id":"676","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","abstract":[{"text":"The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo. We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo. Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation.","lang":"eng"}],"issue":"10","type":"journal_article","date_published":"2017-05-15T00:00:00Z","article_type":"original","page":"1798 - 1806","publication":"Development","citation":{"short":"G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J. Heisenberg, Development 144 (2017) 1798–1806.","mla":"Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” Development, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806, doi:10.1242/dev.144964.","chicago":"Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître, Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” Development. Company of Biologists, 2017. https://doi.org/10.1242/dev.144964.","ama":"Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 2017;144(10):1798-1806. doi:10.1242/dev.144964","apa":"Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W., & Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. Company of Biologists. https://doi.org/10.1242/dev.144964","ieee":"G. Krens et al., “Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation,” Development, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.","ista":"Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 144(10), 1798–1806."},"day":"15","has_accepted_license":"1","article_processing_charge":"No","scopus_import":1},{"scopus_import":1,"day":"27","page":"306 - 317","publication":"Nature Cell Biology","citation":{"ista":"Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M, Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction forces position the neural anlage. Nature Cell Biology. 19, 306–317.","apa":"Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D., … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb3492","ieee":"M. Smutny et al., “Friction forces position the neural anlage,” Nature Cell Biology, vol. 19. Nature Publishing Group, pp. 306–317, 2017.","ama":"Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage. Nature Cell Biology. 2017;19:306-317. doi:10.1038/ncb3492","chicago":"Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural Anlage.” Nature Cell Biology. Nature Publishing Group, 2017. https://doi.org/10.1038/ncb3492.","mla":"Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” Nature Cell Biology, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:10.1038/ncb3492.","short":"M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M. Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 306–317."},"date_published":"2017-03-27T00:00:00Z","type":"journal_article","abstract":[{"lang":"eng","text":"During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo."}],"title":"Friction forces position the neural anlage","status":"public","intvolume":" 19","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"661","oa_version":"Submitted Version","month":"03","publication_identifier":{"issn":["14657392"]},"quality_controlled":"1","project":[{"grant_number":"306589","_id":"25152F3A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Decoding the complexity of turbulence at its origin"},{"_id":"252ABD0A-B435-11E9-9278-68D0E5697425","grant_number":"I 930-B20","call_identifier":"FWF","name":"Control of Epithelial Cell Layer Spreading in Zebrafish"}],"main_file_link":[{"open_access":"1","url":"https://europepmc.org/articles/pmc5635970"}],"external_id":{"pmid":["28346437"]},"oa":1,"acknowledged_ssus":[{"_id":"SSU"}],"language":[{"iso":"eng"}],"doi":"10.1038/ncb3492","ec_funded":1,"publist_id":"7074","publication_status":"published","department":[{"_id":"CaHe"},{"_id":"BjHo"},{"_id":"Bio"}],"publisher":"Nature Publishing Group","year":"2017","pmid":1,"date_created":"2018-12-11T11:47:46Z","date_updated":"2024-03-28T23:30:39Z","volume":19,"author":[{"orcid":"0000-0002-5920-9090","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","last_name":"Smutny","first_name":"Michael","full_name":"Smutny, Michael"},{"full_name":"Ákos, Zsuzsa","first_name":"Zsuzsa","last_name":"Ákos"},{"first_name":"Silvia","last_name":"Grigolon","full_name":"Grigolon, Silvia"},{"first_name":"Shayan","last_name":"Shamipour","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan"},{"full_name":"Ruprecht, Verena","last_name":"Ruprecht","first_name":"Verena"},{"last_name":"Capek","first_name":"Daniel","orcid":"0000-0001-5199-9940","id":"31C42484-F248-11E8-B48F-1D18A9856A87","full_name":"Capek, Daniel"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","last_name":"Behrndt","full_name":"Behrndt, Martin"},{"last_name":"Papusheva","first_name":"Ekaterina","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","full_name":"Papusheva, Ekaterina"},{"full_name":"Tada, Masazumi","last_name":"Tada","first_name":"Masazumi"},{"full_name":"Hof, Björn","orcid":"0000-0003-2057-2754","id":"3A374330-F248-11E8-B48F-1D18A9856A87","last_name":"Hof","first_name":"Björn"},{"last_name":"Vicsek","first_name":"Tamás","full_name":"Vicsek, Tamás"},{"full_name":"Salbreux, Guillaume","first_name":"Guillaume","last_name":"Salbreux"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"50"},{"relation":"dissertation_contains","status":"public","id":"8350"}]}},{"abstract":[{"lang":"eng","text":"Cell-cell contact formation constitutes an essential step in evolution, leading to the differentiation of specialized cell types. However, remarkably little is known about whether and how the interplay between contact formation and fate specification affects development. Here, we identify a positive feedback loop between cell-cell contact duration, morphogen signaling, and mesendoderm cell-fate specification during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for ppl cell-fate specification. We further show that Nodal signaling promotes ppl cell-cell contact duration, generating a positive feedback loop between ppl cell-cell contact duration and cell-fate specification. Finally, by combining mathematical modeling and experimentation, we show that this feedback determines whether anterior axial mesendoderm cells become ppl or, instead, turn into endoderm. Thus, the interdependent activities of cell-cell signaling and contact formation control fate diversification within the developing embryo."}],"issue":"2","type":"journal_article","oa_version":"None","status":"public","title":"An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate","intvolume":" 43","_id":"735","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","day":"23","article_processing_charge":"No","scopus_import":"1","date_published":"2017-10-23T00:00:00Z","page":"198 - 211","publication":"Developmental Cell","citation":{"chicago":"Barone, Vanessa, Moritz Lang, Gabriel Krens, Saurabh Pradhan, Shayan Shamipour, Keisuke Sako, Mateusz K Sikora, Calin C Guet, and Carl-Philipp J Heisenberg. “An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.” Developmental Cell. Cell Press, 2017. https://doi.org/10.1016/j.devcel.2017.09.014.","short":"V. Barone, M. Lang, G. Krens, S. Pradhan, S. Shamipour, K. Sako, M.K. Sikora, C.C. Guet, C.-P.J. Heisenberg, Developmental Cell 43 (2017) 198–211.","mla":"Barone, Vanessa, et al. “An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.” Developmental Cell, vol. 43, no. 2, Cell Press, 2017, pp. 198–211, doi:10.1016/j.devcel.2017.09.014.","apa":"Barone, V., Lang, M., Krens, G., Pradhan, S., Shamipour, S., Sako, K., … Heisenberg, C.-P. J. (2017). An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2017.09.014","ieee":"V. Barone et al., “An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate,” Developmental Cell, vol. 43, no. 2. Cell Press, pp. 198–211, 2017.","ista":"Barone V, Lang M, Krens G, Pradhan S, Shamipour S, Sako K, Sikora MK, Guet CC, Heisenberg C-PJ. 2017. An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. Developmental Cell. 43(2), 198–211.","ama":"Barone V, Lang M, Krens G, et al. An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. Developmental Cell. 2017;43(2):198-211. doi:10.1016/j.devcel.2017.09.014"},"ec_funded":1,"publist_id":"6934","date_updated":"2024-03-28T23:30:39Z","date_created":"2018-12-11T11:48:13Z","volume":43,"author":[{"full_name":"Barone, Vanessa","first_name":"Vanessa","last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367"},{"full_name":"Lang, Moritz","last_name":"Lang","first_name":"Moritz","id":"29E0800A-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","first_name":"Gabriel","full_name":"Krens, Gabriel"},{"last_name":"Pradhan","first_name":"Saurabh","full_name":"Pradhan, Saurabh"},{"last_name":"Shamipour","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan"},{"id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6453-8075","first_name":"Keisuke","last_name":"Sako","full_name":"Sako, Keisuke"},{"full_name":"Sikora, Mateusz K","last_name":"Sikora","first_name":"Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Guet","first_name":"Calin C","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"961"},{"id":"8350","relation":"dissertation_contains","status":"public"}]},"publication_status":"published","publisher":"Cell Press","department":[{"_id":"CaHe"},{"_id":"CaGu"},{"_id":"GaTk"}],"year":"2017","month":"10","publication_identifier":{"issn":["15345807"]},"language":[{"iso":"eng"}],"doi":"10.1016/j.devcel.2017.09.014","isi":1,"quality_controlled":"1","project":[{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"},{"_id":"252DD2A6-B435-11E9-9278-68D0E5697425","grant_number":"I2058","call_identifier":"FWF","name":"Cell segregation in gastrulation: the role of cell fate specification"}],"external_id":{"isi":["000413443700011"]}},{"article_number":"028102","type":"journal_article","abstract":[{"text":"Nonadherent polarized cells have been observed to have a pearlike, elongated shape. Using a minimal model that describes the cell cortex as a thin layer of contractile active gel, we show that the anisotropy of active stresses, controlled by cortical viscosity and filament ordering, can account for this morphology. The predicted shapes can be determined from the flow pattern only; they prove to be independent of the mechanism at the origin of the cortical flow, and are only weakly sensitive to the cytoplasmic rheology. In the case of actin flows resulting from a contractile instability, we propose a phase diagram of three-dimensional cell shapes that encompasses nonpolarized spherical, elongated, as well as oblate shapes, all of which have been observed in experiment.","lang":"eng"}],"issue":"2","publist_id":"6095","_id":"1239","acknowledgement":"V. R. acknowledges support by the Austrian Science Fund (FWF): (Grant No. T560-B17).","year":"2016","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publication_status":"published","title":"Cortical flow-driven shapes of nonadherent cells","status":"public","publisher":"American Physical Society","intvolume":" 116","department":[{"_id":"CaHe"}],"author":[{"first_name":"Andrew","last_name":"Callan Jones","full_name":"Callan Jones, Andrew"},{"full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633"},{"full_name":"Wieser, Stefan","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","last_name":"Wieser","first_name":"Stefan"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"full_name":"Voituriez, Raphaël","last_name":"Voituriez","first_name":"Raphaël"}],"date_created":"2018-12-11T11:50:53Z","date_updated":"2021-01-12T06:49:19Z","volume":116,"oa_version":"None","scopus_import":1,"day":"15","month":"01","publication":"Physical Review Letters","citation":{"chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Cortical Flow-Driven Shapes of Nonadherent Cells.” Physical Review Letters. American Physical Society, 2016. https://doi.org/10.1103/PhysRevLett.116.028102.","mla":"Callan Jones, Andrew, et al. “Cortical Flow-Driven Shapes of Nonadherent Cells.” Physical Review Letters, vol. 116, no. 2, 028102, American Physical Society, 2016, doi:10.1103/PhysRevLett.116.028102.","short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 116 (2016).","ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. 116(2), 028102.","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., & Voituriez, R. (2016). Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.116.028102","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Cortical flow-driven shapes of nonadherent cells,” Physical Review Letters, vol. 116, no. 2. American Physical Society, 2016.","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. 2016;116(2). doi:10.1103/PhysRevLett.116.028102"},"quality_controlled":"1","project":[{"grant_number":"T 560-B17","_id":"2529486C-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation"}],"doi":"10.1103/PhysRevLett.116.028102","date_published":"2016-01-15T00:00:00Z","language":[{"iso":"eng"}]},{"file_date_updated":"2020-07-14T12:44:41Z","publist_id":"6079","publication_status":"published","publisher":"Biophysical Society","department":[{"_id":"CaHe"}],"year":"2016","acknowledgement":"S.W.G. acknowledges support by grant no. 281903 from the European Research Council and by grant No. GR-7271/2-1 from the Deutsche Forschungsgemeinschaft. S.W.G. and C.-P.H. acknowledge support through a grant from the Fonds zur Förderung der Wissenschaftlichen Forschung and the Deutsche Forschungsgemeinschaft (No. I930-B20). We are grateful to Daniel Dickinson for providing the LP133 C. elegans strain. We thank G. Salbreux, V. K. Krishnamurthy, and J. S. Bois for fruitful discussions.","date_updated":"2021-01-12T06:49:23Z","date_created":"2018-12-11T11:50:56Z","volume":110,"author":[{"full_name":"Saha, Arnab","last_name":"Saha","first_name":"Arnab"},{"first_name":"Masatoshi","last_name":"Nishikawa","full_name":"Nishikawa, Masatoshi"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt","first_name":"Martin","full_name":"Behrndt, Martin"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Julicher, Frank","first_name":"Frank","last_name":"Julicher"},{"first_name":"Stephan","last_name":"Grill","full_name":"Grill, Stephan"}],"month":"03","quality_controlled":"1","project":[{"name":"Control of Epithelial Cell Layer Spreading in Zebrafish","call_identifier":"FWF","grant_number":"I 930-B20","_id":"252ABD0A-B435-11E9-9278-68D0E5697425"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"language":[{"iso":"eng"}],"doi":"10.1016/j.bpj.2016.02.013","type":"journal_article","abstract":[{"text":"Actin and myosin assemble into a thin layer of a highly dynamic network underneath the membrane of eukaryotic cells. This network generates the forces that drive cell- and tissue-scale morphogenetic processes. The effective material properties of this active network determine large-scale deformations and other morphogenetic events. For example, the characteristic time of stress relaxation (the Maxwell time τM) in the actomyosin sets the timescale of large-scale deformation of the cortex. Similarly, the characteristic length of stress propagation (the hydrodynamic length λ) sets the length scale of slow deformations, and a large hydrodynamic length is a prerequisite for long-ranged cortical flows. Here we introduce a method to determine physical parameters of the actomyosin cortical layer in vivo directly from laser ablation experiments. For this we investigate the cortical response to laser ablation in the one-cell-stage Caenorhabditis elegans embryo and in the gastrulating zebrafish embryo. These responses can be interpreted using a coarse-grained physical description of the cortex in terms of a two-dimensional thin film of an active viscoelastic gel. To determine the Maxwell time τM, the hydrodynamic length λ, the ratio of active stress ζΔμ, and per-area friction γ, we evaluated the response to laser ablation in two different ways: by quantifying flow and density fields as a function of space and time, and by determining the time evolution of the shape of the ablated region. Importantly, both methods provide best-fit physical parameters that are in close agreement with each other and that are similar to previous estimates in the two systems. Our method provides an accurate and robust means for measuring physical parameters of the actomyosin cortical layer. It can be useful for investigations of actomyosin mechanics at the cellular-scale, but also for providing insights into the active mechanics processes that govern tissue-scale morphogenesis.","lang":"eng"}],"issue":"6","ddc":["572","576"],"title":"Determining physical properties of the cell cortex","status":"public","intvolume":" 110","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"1249","oa_version":"Published Version","file":[{"creator":"system","content_type":"application/pdf","file_size":1965645,"access_level":"open_access","file_name":"IST-2016-706-v1+1_1-s2.0-S0006349516001582-main.pdf","checksum":"c408cf2e25a25c8d711cffea524bda55","date_updated":"2020-07-14T12:44:41Z","date_created":"2018-12-12T10:10:54Z","file_id":"4845","relation":"main_file"}],"pubrep_id":"706","scopus_import":1,"day":"29","has_accepted_license":"1","page":"1421 - 1429","publication":"Biophysical Journal","citation":{"ama":"Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. Determining physical properties of the cell cortex. Biophysical Journal. 2016;110(6):1421-1429. doi:10.1016/j.bpj.2016.02.013","ista":"Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. 2016. Determining physical properties of the cell cortex. Biophysical Journal. 110(6), 1421–1429.","ieee":"A. Saha, M. Nishikawa, M. Behrndt, C.-P. J. Heisenberg, F. Julicher, and S. Grill, “Determining physical properties of the cell cortex,” Biophysical Journal, vol. 110, no. 6. Biophysical Society, pp. 1421–1429, 2016.","apa":"Saha, A., Nishikawa, M., Behrndt, M., Heisenberg, C.-P. J., Julicher, F., & Grill, S. (2016). Determining physical properties of the cell cortex. Biophysical Journal. Biophysical Society. https://doi.org/10.1016/j.bpj.2016.02.013","mla":"Saha, Arnab, et al. “Determining Physical Properties of the Cell Cortex.” Biophysical Journal, vol. 110, no. 6, Biophysical Society, 2016, pp. 1421–29, doi:10.1016/j.bpj.2016.02.013.","short":"A. Saha, M. Nishikawa, M. Behrndt, C.-P.J. Heisenberg, F. Julicher, S. Grill, Biophysical Journal 110 (2016) 1421–1429.","chicago":"Saha, Arnab, Masatoshi Nishikawa, Martin Behrndt, Carl-Philipp J Heisenberg, Frank Julicher, and Stephan Grill. “Determining Physical Properties of the Cell Cortex.” Biophysical Journal. Biophysical Society, 2016. https://doi.org/10.1016/j.bpj.2016.02.013."},"date_published":"2016-03-29T00:00:00Z"},{"pubrep_id":"695","oa_version":"Published Version","file":[{"file_id":"5002","relation":"main_file","checksum":"0bfa484ac69a0a560fb9a4589aeda7f6","date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:13:20Z","access_level":"open_access","file_name":"IST-2016-695-v1+1_s12915-016-0294-x.pdf","creator":"system","file_size":1875695,"content_type":"application/pdf"}],"_id":"1271","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","status":"public","ddc":["572","576"],"title":"Steering cell migration by alternating blebs and actin-rich protrusions","intvolume":" 14","abstract":[{"lang":"eng","text":"Background: High directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low directional persistence, and the control and function of directional persistence during cell migration in three-dimensional environments are poorly understood. Results: Here, we use mesendoderm progenitors migrating during zebrafish gastrulation as a model system to investigate the control of directional persistence during migration in vivo. We show that progenitor cells alternate persistent run phases with tumble phases that result in cell reorientation. Runs are characterized by the formation of directed actin-rich protrusions and tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions or blebs leads to longer or shorter run phases, respectively. Importantly, both reducing and increasing run phases result in larger spatial dispersion of the cells, indicative of reduced migration precision. A physical model quantitatively recapitulating the migratory behavior of mesendoderm progenitors indicates that the ratio of tumbling to run times, and thus the specific degree of directional persistence of migration, are critical for optimizing migration precision. Conclusions: Together, our experiments and model provide mechanistic insight into the control of migration directionality for cells moving in three-dimensional environments that combine different protrusion types, whereby the proportion of blebs to actin-rich protrusions determines the directional persistence and precision of movement by regulating the ratio of tumbling to run times."}],"issue":"1","type":"journal_article","date_published":"2016-09-02T00:00:00Z","publication":"BMC Biology","citation":{"ista":"Diz Muñoz A, Romanczuk P, Yu W, Bergert M, Ivanovitch K, Salbreux G, Heisenberg C-PJ, Paluch E. 2016. Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. 14(1), 74.","ieee":"A. Diz Muñoz et al., “Steering cell migration by alternating blebs and actin-rich protrusions,” BMC Biology, vol. 14, no. 1. BioMed Central, 2016.","apa":"Diz Muñoz, A., Romanczuk, P., Yu, W., Bergert, M., Ivanovitch, K., Salbreux, G., … Paluch, E. (2016). Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. BioMed Central. https://doi.org/10.1186/s12915-016-0294-x","ama":"Diz Muñoz A, Romanczuk P, Yu W, et al. Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. 2016;14(1). doi:10.1186/s12915-016-0294-x","chicago":"Diz Muñoz, Alba, Pawel Romanczuk, Weimiao Yu, Martin Bergert, Kenzo Ivanovitch, Guillame Salbreux, Carl-Philipp J Heisenberg, and Ewa Paluch. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” BMC Biology. BioMed Central, 2016. https://doi.org/10.1186/s12915-016-0294-x.","mla":"Diz Muñoz, Alba, et al. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” BMC Biology, vol. 14, no. 1, 74, BioMed Central, 2016, doi:10.1186/s12915-016-0294-x.","short":"A. Diz Muñoz, P. Romanczuk, W. Yu, M. Bergert, K. Ivanovitch, G. Salbreux, C.-P.J. Heisenberg, E. Paluch, BMC Biology 14 (2016)."},"day":"02","has_accepted_license":"1","scopus_import":1,"author":[{"last_name":"Diz Muñoz","first_name":"Alba","full_name":"Diz Muñoz, Alba"},{"full_name":"Romanczuk, Pawel","first_name":"Pawel","last_name":"Romanczuk"},{"last_name":"Yu","first_name":"Weimiao","full_name":"Yu, Weimiao"},{"full_name":"Bergert, Martin","first_name":"Martin","last_name":"Bergert"},{"first_name":"Kenzo","last_name":"Ivanovitch","full_name":"Ivanovitch, Kenzo"},{"full_name":"Salbreux, Guillame","first_name":"Guillame","last_name":"Salbreux"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Paluch, Ewa","first_name":"Ewa","last_name":"Paluch"}],"date_created":"2018-12-11T11:51:04Z","date_updated":"2021-01-12T06:49:32Z","volume":14,"acknowledgement":"We thank K. Lee, C. Norden, A. Webb, and the members of the Paluch lab for\r\ncomments on the manuscript. We are grateful to P. Rørth and Peter Dieterich\r\nfor discussions, S. Ares, Y. Arboleda-Estudillo and S. Schneider for technical help,\r\nM. Biro for help with programming, and the BIOTEC/MPI-CBG and IST zebrafish\r\nand imaging facilities for help and advice at various stages of this project. This work was supported by the Max Planck Society, the Medical Research Council UK (core funding to the MRC LMCB), and by grants from the Polish Ministry of Science and Higher Education (454/N-MPG/2009/0) to EKP, the Deutsche Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EKP, a A*Star JCO career development award (12302FG010) to WY and a Damon Runyon fellowship award to ADM (DRG 2157-12). This work was also supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001317), the UK Medical Research Council (FC001317), and the Wellcome Trust (FC001317) to GS.","year":"2016","publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"BioMed Central","file_date_updated":"2020-07-14T12:44:42Z","publist_id":"6049","article_number":"74","doi":"10.1186/s12915-016-0294-x","acknowledged_ssus":[{"_id":"LifeSc"}],"language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"quality_controlled":"1","project":[{"name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo","_id":"252064B8-B435-11E9-9278-68D0E5697425","grant_number":"HE_3231/6-1"}],"month":"09"},{"language":[{"iso":"eng"}],"doi":"10.1103/PhysRevLett.117.139802","date_published":"2016-09-22T00:00:00Z","quality_controlled":"1","publication":"Physical Review Letters","citation":{"chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Callan-Jones et Al. Reply.” Physical Review Letters. American Physical Society, 2016. https://doi.org/10.1103/PhysRevLett.117.139802.","short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 117 (2016).","mla":"Callan Jones, Andrew, et al. “Callan-Jones et Al. Reply.” Physical Review Letters, vol. 117, no. 13, 139802, American Physical Society, 2016, doi:10.1103/PhysRevLett.117.139802.","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Callan-Jones et al. Reply,” Physical Review Letters, vol. 117, no. 13. American Physical Society, 2016.","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., & Voituriez, R. (2016). Callan-Jones et al. Reply. Physical Review Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.117.139802","ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Callan-Jones et al. Reply. Physical Review Letters. 117(13), 139802.","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Callan-Jones et al. Reply. Physical Review Letters. 2016;117(13). doi:10.1103/PhysRevLett.117.139802"},"day":"22","month":"09","scopus_import":1,"date_updated":"2021-01-12T06:49:33Z","date_created":"2018-12-11T11:51:05Z","oa_version":"None","volume":117,"author":[{"full_name":"Callan Jones, Andrew","last_name":"Callan Jones","first_name":"Andrew"},{"orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","last_name":"Ruprecht","first_name":"Verena","full_name":"Ruprecht, Verena"},{"full_name":"Wieser, Stefan","first_name":"Stefan","last_name":"Wieser","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2670-2217"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"full_name":"Voituriez, Raphaël","first_name":"Raphaël","last_name":"Voituriez"}],"publication_status":"published","status":"public","title":"Callan-Jones et al. Reply","publisher":"American Physical Society","intvolume":" 117","department":[{"_id":"CaHe"}],"_id":"1275","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","year":"2016","issue":"13","publist_id":"6041","article_number":"139802","type":"journal_article"},{"page":"493 - 506","quality_controlled":"1","citation":{"ista":"Schwayer C, Sikora MK, Slovakova J, Kardos R, Heisenberg C-PJ. 2016. Actin rings of power. Developmental Cell. 37(6), 493–506.","ieee":"C. Schwayer, M. K. Sikora, J. Slovakova, R. Kardos, and C.-P. J. Heisenberg, “Actin rings of power,” Developmental Cell, vol. 37, no. 6. Cell Press, pp. 493–506, 2016.","apa":"Schwayer, C., Sikora, M. K., Slovakova, J., Kardos, R., & Heisenberg, C.-P. J. (2016). Actin rings of power. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2016.05.024","ama":"Schwayer C, Sikora MK, Slovakova J, Kardos R, Heisenberg C-PJ. Actin rings of power. Developmental Cell. 2016;37(6):493-506. doi:10.1016/j.devcel.2016.05.024","chicago":"Schwayer, Cornelia, Mateusz K Sikora, Jana Slovakova, Roland Kardos, and Carl-Philipp J Heisenberg. “Actin Rings of Power.” Developmental Cell. Cell Press, 2016. https://doi.org/10.1016/j.devcel.2016.05.024.","mla":"Schwayer, Cornelia, et al. “Actin Rings of Power.” Developmental Cell, vol. 37, no. 6, Cell Press, 2016, pp. 493–506, doi:10.1016/j.devcel.2016.05.024.","short":"C. Schwayer, M.K. Sikora, J. Slovakova, R. Kardos, C.-P.J. Heisenberg, Developmental Cell 37 (2016) 493–506."},"publication":"Developmental Cell","language":[{"iso":"eng"}],"date_published":"2016-06-20T00:00:00Z","doi":"10.1016/j.devcel.2016.05.024","scopus_import":1,"day":"20","month":"06","publisher":"Cell Press","department":[{"_id":"CaHe"}],"intvolume":" 37","publication_status":"published","title":"Actin rings of power","status":"public","year":"2016","_id":"1096","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","oa_version":"None","volume":37,"date_created":"2018-12-11T11:50:07Z","date_updated":"2023-09-07T12:56:41Z","related_material":{"record":[{"id":"7186","status":"public","relation":"part_of_dissertation"}]},"author":[{"full_name":"Schwayer, Cornelia","id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226","first_name":"Cornelia","last_name":"Schwayer"},{"full_name":"Sikora, Mateusz K","last_name":"Sikora","first_name":"Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Jana","last_name":"Slovakova","id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87","full_name":"Slovakova, Jana"},{"full_name":"Kardos, Roland","last_name":"Kardos","first_name":"Roland","id":"4039350E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"type":"journal_article","issue":"6","publist_id":"6279"},{"publisher":"Cell Press","department":[{"_id":"CaHe"},{"_id":"HaJa"}],"publication_status":"published","year":"2016","acknowledgement":"We are grateful to members of the C.-P.H. and H.J. labs for discussions, R. Hauschild and the different Scientific Service Units at IST Austria for technical help, M. Dravecka for performing initial experiments, A. Schier for reading an earlier version of the manuscript, K.W. Rogers for technical help, and C. Hill, A. Bruce, and L. Solnica-Krezel for sending plasmids. This work was supported by grants from the Austrian Science Foundation (FWF): (T560-B17) and (I 812-B12) to V.R. and C.-P.H., and from the European Union (EU FP7): (6275) to H.J. A.I.-P. is supported by a Ramon Areces fellowship.","volume":16,"date_updated":"2024-03-28T23:30:26Z","date_created":"2018-12-11T11:50:08Z","related_material":{"record":[{"id":"961","status":"public","relation":"dissertation_contains"},{"status":"public","relation":"dissertation_contains","id":"50"}]},"author":[{"full_name":"Sako, Keisuke","orcid":"0000-0002-6453-8075","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","last_name":"Sako","first_name":"Keisuke"},{"first_name":"Saurabh","last_name":"Pradhan","full_name":"Pradhan, Saurabh"},{"full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone","first_name":"Vanessa"},{"full_name":"Inglés Prieto, Álvaro","first_name":"Álvaro","last_name":"Inglés Prieto","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5409-8571"},{"last_name":"Mueller","first_name":"Patrick","full_name":"Mueller, Patrick"},{"first_name":"Verena","last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633","full_name":"Ruprecht, Verena"},{"full_name":"Capek, Daniel","id":"31C42484-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5199-9940","first_name":"Daniel","last_name":"Capek"},{"first_name":"Sanjeev","last_name":"Galande","full_name":"Galande, Sanjeev"},{"full_name":"Janovjak, Harald L","first_name":"Harald L","last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8023-9315"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"publist_id":"6275","ec_funded":1,"file_date_updated":"2018-12-12T10:11:04Z","project":[{"_id":"2529486C-B435-11E9-9278-68D0E5697425","grant_number":"T 560-B17","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","call_identifier":"FWF"},{"grant_number":"I 812-B12","_id":"2527D5CC-B435-11E9-9278-68D0E5697425","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation","call_identifier":"FWF"},{"_id":"25548C20-B435-11E9-9278-68D0E5697425","grant_number":"303564","call_identifier":"FP7","name":"Microbial Ion Channels for Synthetic Neurobiology"}],"quality_controlled":"1","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"SSU"}],"doi":"10.1016/j.celrep.2016.06.036","month":"07","intvolume":" 16","title":"Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation","ddc":["570","576"],"status":"public","_id":"1100","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","file":[{"date_created":"2018-12-12T10:11:04Z","date_updated":"2018-12-12T10:11:04Z","relation":"main_file","file_id":"4857","file_size":3921947,"content_type":"application/pdf","creator":"system","file_name":"IST-2017-754-v1+1_1-s2.0-S2211124716307768-main.pdf","access_level":"open_access"}],"pubrep_id":"754","type":"journal_article","issue":"3","abstract":[{"text":"During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation.","lang":"eng"}],"page":"866 - 877","citation":{"chicago":"Sako, Keisuke, Saurabh Pradhan, Vanessa Barone, Álvaro Inglés Prieto, Patrick Mueller, Verena Ruprecht, Daniel Capek, Sanjeev Galande, Harald L Janovjak, and Carl-Philipp J Heisenberg. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” Cell Reports. Cell Press, 2016. https://doi.org/10.1016/j.celrep.2016.06.036.","mla":"Sako, Keisuke, et al. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” Cell Reports, vol. 16, no. 3, Cell Press, 2016, pp. 866–77, doi:10.1016/j.celrep.2016.06.036.","short":"K. Sako, S. Pradhan, V. Barone, Á. Inglés Prieto, P. Mueller, V. Ruprecht, D. Capek, S. Galande, H.L. Janovjak, C.-P.J. Heisenberg, Cell Reports 16 (2016) 866–877.","ista":"Sako K, Pradhan S, Barone V, Inglés Prieto Á, Mueller P, Ruprecht V, Capek D, Galande S, Janovjak HL, Heisenberg C-PJ. 2016. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. 16(3), 866–877.","ieee":"K. Sako et al., “Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation,” Cell Reports, vol. 16, no. 3. Cell Press, pp. 866–877, 2016.","apa":"Sako, K., Pradhan, S., Barone, V., Inglés Prieto, Á., Mueller, P., Ruprecht, V., … Heisenberg, C.-P. J. (2016). Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2016.06.036","ama":"Sako K, Pradhan S, Barone V, et al. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. 2016;16(3):866-877. doi:10.1016/j.celrep.2016.06.036"},"publication":"Cell Reports","date_published":"2016-07-19T00:00:00Z","scopus_import":1,"has_accepted_license":"1","day":"19"},{"publist_id":"5618","issue":"2","ec_funded":1,"abstract":[{"lang":"eng","text":"Cell movement has essential functions in development, immunity, and cancer. Various cell migration patterns have been reported, but no general rule has emerged so far. Here, we show on the basis of experimental data in vitro and in vivo that cell persistence, which quantifies the straightness of trajectories, is robustly coupled to cell migration speed. We suggest that this universal coupling constitutes a generic law of cell migration, which originates in the advection of polarity cues by an actin cytoskeleton undergoing flows at the cellular scale. Our analysis relies on a theoretical model that we validate by measuring the persistence of cells upon modulation of actin flow speeds and upon optogenetic manipulation of the binding of an actin regulator to actin filaments. Beyond the quantitative prediction of the coupling, the model yields a generic phase diagram of cellular trajectories, which recapitulates the full range of observed migration patterns."}],"type":"journal_article","volume":161,"oa_version":"None","date_updated":"2021-01-12T06:51:33Z","date_created":"2018-12-11T11:52:41Z","author":[{"full_name":"Maiuri, Paolo","last_name":"Maiuri","first_name":"Paolo"},{"full_name":"Rupprecht, Jean","first_name":"Jean","last_name":"Rupprecht"},{"full_name":"Wieser, Stefan","last_name":"Wieser","first_name":"Stefan","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Verena","last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633","full_name":"Ruprecht, Verena"},{"full_name":"Bénichou, Olivier","last_name":"Bénichou","first_name":"Olivier"},{"first_name":"Nicolas","last_name":"Carpi","full_name":"Carpi, Nicolas"},{"full_name":"Coppey, Mathieu","last_name":"Coppey","first_name":"Mathieu"},{"last_name":"De Beco","first_name":"Simon","full_name":"De Beco, Simon"},{"first_name":"Nir","last_name":"Gov","full_name":"Gov, Nir"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Carolina","last_name":"Lage Crespo","full_name":"Lage Crespo, Carolina"},{"first_name":"Franziska","last_name":"Lautenschlaeger","full_name":"Lautenschlaeger, Franziska"},{"full_name":"Le Berre, Maël","first_name":"Maël","last_name":"Le Berre"},{"full_name":"Lennon Duménil, Ana","first_name":"Ana","last_name":"Lennon Duménil"},{"full_name":"Raab, Matthew","first_name":"Matthew","last_name":"Raab"},{"full_name":"Thiam, Hawa","last_name":"Thiam","first_name":"Hawa"},{"last_name":"Piel","first_name":"Matthieu","full_name":"Piel, Matthieu"},{"full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt"},{"full_name":"Voituriez, Raphaël","last_name":"Voituriez","first_name":"Raphaël"}],"publisher":"Cell Press","intvolume":" 161","department":[{"_id":"MiSi"},{"_id":"CaHe"}],"status":"public","publication_status":"published","title":"Actin flows mediate a universal coupling between cell speed and cell persistence","_id":"1553","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2015","day":"09","month":"04","scopus_import":1,"language":[{"iso":"eng"}],"date_published":"2015-04-09T00:00:00Z","doi":"10.1016/j.cell.2015.01.056","page":"374 - 386","project":[{"call_identifier":"FWF","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","_id":"2529486C-B435-11E9-9278-68D0E5697425","grant_number":"T 560-B17"},{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7","_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556"},{"name":"Cell migration in complex environments: from in vivo experiments to theoretical models","_id":"25ABD200-B435-11E9-9278-68D0E5697425","grant_number":"RGP0058/2011"}],"quality_controlled":"1","citation":{"chicago":"Maiuri, Paolo, Jean Rupprecht, Stefan Wieser, Verena Ruprecht, Olivier Bénichou, Nicolas Carpi, Mathieu Coppey, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” Cell. Cell Press, 2015. https://doi.org/10.1016/j.cell.2015.01.056.","mla":"Maiuri, Paolo, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” Cell, vol. 161, no. 2, Cell Press, 2015, pp. 374–86, doi:10.1016/j.cell.2015.01.056.","short":"P. Maiuri, J. Rupprecht, S. Wieser, V. Ruprecht, O. Bénichou, N. Carpi, M. Coppey, S. De Beco, N. Gov, C.-P.J. Heisenberg, C. Lage Crespo, F. Lautenschlaeger, M. Le Berre, A. Lennon Duménil, M. Raab, H. Thiam, M. Piel, M.K. Sixt, R. Voituriez, Cell 161 (2015) 374–386.","ista":"Maiuri P, Rupprecht J, Wieser S, Ruprecht V, Bénichou O, Carpi N, Coppey M, De Beco S, Gov N, Heisenberg C-PJ, Lage Crespo C, Lautenschlaeger F, Le Berre M, Lennon Duménil A, Raab M, Thiam H, Piel M, Sixt MK, Voituriez R. 2015. Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. 161(2), 374–386.","apa":"Maiuri, P., Rupprecht, J., Wieser, S., Ruprecht, V., Bénichou, O., Carpi, N., … Voituriez, R. (2015). Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.056","ieee":"P. Maiuri et al., “Actin flows mediate a universal coupling between cell speed and cell persistence,” Cell, vol. 161, no. 2. Cell Press, pp. 374–386, 2015.","ama":"Maiuri P, Rupprecht J, Wieser S, et al. Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. 2015;161(2):374-386. doi:10.1016/j.cell.2015.01.056"},"publication":"Cell"},{"type":"journal_article","publist_id":"5590","issue":"3","abstract":[{"lang":"eng","text":"In animal embryos, morphogen gradients determine tissue patterning and morphogenesis. Shyer et al. provide evidence that, during vertebrate gut formation, tissue folding generates graded activity of signals required for subsequent steps of gut growth and differentiation, thereby revealing an intriguing link between tissue morphogenesis and morphogen gradient formation."}],"department":[{"_id":"ToBo"},{"_id":"CaHe"}],"publisher":"Cell Press","intvolume":" 161","title":"Gradients are shaping up","publication_status":"published","status":"public","year":"2015","_id":"1581","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","oa_version":"None","volume":161,"date_updated":"2022-08-25T13:56:10Z","date_created":"2018-12-11T11:52:50Z","author":[{"full_name":"Bollenbach, Mark Tobias","last_name":"Bollenbach","first_name":"Mark Tobias","orcid":"0000-0003-4398-476X","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"scopus_import":"1","article_processing_charge":"No","day":"23","month":"04","page":"431 - 432","quality_controlled":"1","citation":{"short":"M.T. Bollenbach, C.-P.J. Heisenberg, Cell 161 (2015) 431–432.","mla":"Bollenbach, Mark Tobias, and Carl-Philipp J. Heisenberg. “Gradients Are Shaping Up.” Cell, vol. 161, no. 3, Cell Press, 2015, pp. 431–32, doi:10.1016/j.cell.2015.04.009.","chicago":"Bollenbach, Mark Tobias, and Carl-Philipp J Heisenberg. “Gradients Are Shaping Up.” Cell. Cell Press, 2015. https://doi.org/10.1016/j.cell.2015.04.009.","ama":"Bollenbach MT, Heisenberg C-PJ. Gradients are shaping up. Cell. 2015;161(3):431-432. doi:10.1016/j.cell.2015.04.009","ieee":"M. T. Bollenbach and C.-P. J. Heisenberg, “Gradients are shaping up,” Cell, vol. 161, no. 3. Cell Press, pp. 431–432, 2015.","apa":"Bollenbach, M. T., & Heisenberg, C.-P. J. (2015). Gradients are shaping up. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.04.009","ista":"Bollenbach MT, Heisenberg C-PJ. 2015. Gradients are shaping up. Cell. 161(3), 431–432."},"publication":"Cell","language":[{"iso":"eng"}],"date_published":"2015-04-23T00:00:00Z","doi":"10.1016/j.cell.2015.04.009"},{"publist_id":"5289","author":[{"first_name":"Sean","last_name":"Porazinski","full_name":"Porazinski, Sean"},{"full_name":"Wang, Huijia","first_name":"Huijia","last_name":"Wang"},{"first_name":"Yoichi","last_name":"Asaoka","full_name":"Asaoka, Yoichi"},{"first_name":"Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","full_name":"Behrndt, Martin"},{"last_name":"Miyamoto","first_name":"Tatsuo","full_name":"Miyamoto, Tatsuo"},{"full_name":"Morita, Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","first_name":"Hitoshi","last_name":"Morita"},{"last_name":"Hata","first_name":"Shoji","full_name":"Hata, Shoji"},{"full_name":"Sasaki, Takashi","first_name":"Takashi","last_name":"Sasaki"},{"full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","last_name":"Krens","first_name":"Gabriel"},{"first_name":"Yumi","last_name":"Osada","full_name":"Osada, Yumi"},{"first_name":"Satoshi","last_name":"Asaka","full_name":"Asaka, Satoshi"},{"full_name":"Momoi, Akihiro","last_name":"Momoi","first_name":"Akihiro"},{"last_name":"Linton","first_name":"Sarah","full_name":"Linton, Sarah"},{"last_name":"Miesfeld","first_name":"Joel","full_name":"Miesfeld, Joel"},{"first_name":"Brian","last_name":"Link","full_name":"Link, Brian"},{"full_name":"Senga, Takeshi","last_name":"Senga","first_name":"Takeshi"},{"full_name":"Castillo Morales, Atahualpa","last_name":"Castillo Morales","first_name":"Atahualpa"},{"last_name":"Urrutia","first_name":"Araxi","full_name":"Urrutia, Araxi"},{"first_name":"Nobuyoshi","last_name":"Shimizu","full_name":"Shimizu, Nobuyoshi"},{"full_name":"Nagase, Hideaki","first_name":"Hideaki","last_name":"Nagase"},{"full_name":"Matsuura, Shinya","first_name":"Shinya","last_name":"Matsuura"},{"last_name":"Bagby","first_name":"Stefan","full_name":"Bagby, Stefan"},{"first_name":"Hisato","last_name":"Kondoh","full_name":"Kondoh, Hisato"},{"full_name":"Nishina, Hiroshi","first_name":"Hiroshi","last_name":"Nishina"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"last_name":"Furutani Seiki","first_name":"Makoto","full_name":"Furutani Seiki, Makoto"}],"volume":521,"date_created":"2018-12-11T11:54:10Z","date_updated":"2021-01-12T06:53:23Z","pmid":1,"year":"2015","department":[{"_id":"CaHe"}],"publisher":"Nature Publishing Group","publication_status":"published","month":"03","doi":"10.1038/nature14215","language":[{"iso":"eng"}],"external_id":{"pmid":["25778702"]},"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720436/"}],"oa":1,"quality_controlled":"1","issue":"7551","abstract":[{"lang":"eng","text":"Vertebrates have a unique 3D body shape in which correct tissue and organ shape and alignment are essential for function. For example, vision requires the lens to be centred in the eye cup which must in turn be correctly positioned in the head. Tissue morphogenesis depends on force generation, force transmission through the tissue, and response of tissues and extracellular matrix to force. Although a century ago D'Arcy Thompson postulated that terrestrial animal body shapes are conditioned by gravity, there has been no animal model directly demonstrating how the aforementioned mechano-morphogenetic processes are coordinated to generate a body shape that withstands gravity. Here we report a unique medaka fish (Oryzias latipes) mutant, hirame (hir), which is sensitive to deformation by gravity. hir embryos display a markedly flattened body caused by mutation of YAP, a nuclear executor of Hippo signalling that regulates organ size. We show that actomyosin-mediated tissue tension is reduced in hir embryos, leading to tissue flattening and tissue misalignment, both of which contribute to body flattening. By analysing YAP function in 3D spheroids of human cells, we identify the Rho GTPase activating protein ARHGAP18 as an effector of YAP in controlling tissue tension. Together, these findings reveal a previously unrecognised function of YAP in regulating tissue shape and alignment required for proper 3D body shape. Understanding this morphogenetic function of YAP could facilitate the use of embryonic stem cells to generate complex organs requiring correct alignment of multiple tissues. "}],"type":"journal_article","oa_version":"Submitted Version","user_id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","_id":"1817","intvolume":" 521","title":"YAP is essential for tissue tension to ensure vertebrate 3D body shape","status":"public","day":"16","scopus_import":1,"date_published":"2015-03-16T00:00:00Z","citation":{"ieee":"S. Porazinski et al., “YAP is essential for tissue tension to ensure vertebrate 3D body shape,” Nature, vol. 521, no. 7551. Nature Publishing Group, pp. 217–221, 2015.","apa":"Porazinski, S., Wang, H., Asaoka, Y., Behrndt, M., Miyamoto, T., Morita, H., … Furutani Seiki, M. (2015). YAP is essential for tissue tension to ensure vertebrate 3D body shape. Nature. Nature Publishing Group. https://doi.org/10.1038/nature14215","ista":"Porazinski S, Wang H, Asaoka Y, Behrndt M, Miyamoto T, Morita H, Hata S, Sasaki T, Krens G, Osada Y, Asaka S, Momoi A, Linton S, Miesfeld J, Link B, Senga T, Castillo Morales A, Urrutia A, Shimizu N, Nagase H, Matsuura S, Bagby S, Kondoh H, Nishina H, Heisenberg C-PJ, Furutani Seiki M. 2015. YAP is essential for tissue tension to ensure vertebrate 3D body shape. Nature. 521(7551), 217–221.","ama":"Porazinski S, Wang H, Asaoka Y, et al. YAP is essential for tissue tension to ensure vertebrate 3D body shape. Nature. 2015;521(7551):217-221. doi:10.1038/nature14215","chicago":"Porazinski, Sean, Huijia Wang, Yoichi Asaoka, Martin Behrndt, Tatsuo Miyamoto, Hitoshi Morita, Shoji Hata, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate 3D Body Shape.” Nature. Nature Publishing Group, 2015. https://doi.org/10.1038/nature14215.","short":"S. Porazinski, H. Wang, Y. Asaoka, M. Behrndt, T. Miyamoto, H. Morita, S. Hata, T. Sasaki, G. Krens, Y. Osada, S. Asaka, A. Momoi, S. Linton, J. Miesfeld, B. Link, T. Senga, A. Castillo Morales, A. Urrutia, N. Shimizu, H. Nagase, S. Matsuura, S. Bagby, H. Kondoh, H. Nishina, C.-P.J. Heisenberg, M. Furutani Seiki, Nature 521 (2015) 217–221.","mla":"Porazinski, Sean, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate 3D Body Shape.” Nature, vol. 521, no. 7551, Nature Publishing Group, 2015, pp. 217–21, doi:10.1038/nature14215."},"publication":"Nature","page":"217 - 221"},{"acknowledgement":"We would like to thank R. Hausschild and E. Papusheva for technical assistance and the service facilities at the IST Austria for continuous support. The caRhoA plasmid was a kind gift of T. Kudoh and A. Takesono. We thank M. Piel and E. Paluch for exchanging unpublished data. ","year":"2015","publication_status":"published","publisher":"Cell Press","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"author":[{"full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633"},{"full_name":"Wieser, Stefan","first_name":"Stefan","last_name":"Wieser","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2670-2217"},{"last_name":"Callan Jones","first_name":"Andrew","full_name":"Callan Jones, Andrew"},{"full_name":"Smutny, Michael","last_name":"Smutny","first_name":"Michael","orcid":"0000-0002-5920-9090","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87"},{"id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","last_name":"Morita","first_name":"Hitoshi","full_name":"Morita, Hitoshi"},{"full_name":"Sako, Keisuke","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6453-8075","first_name":"Keisuke","last_name":"Sako"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367","first_name":"Vanessa","last_name":"Barone","full_name":"Barone, Vanessa"},{"last_name":"Ritsch Marte","first_name":"Monika","full_name":"Ritsch Marte, Monika"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"full_name":"Voituriez, Raphaël","first_name":"Raphaël","last_name":"Voituriez"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"record":[{"id":"961","relation":"dissertation_contains","status":"public"}]},"date_created":"2018-12-11T11:52:35Z","date_updated":"2023-09-07T12:05:08Z","volume":160,"file_date_updated":"2020-07-14T12:45:01Z","publist_id":"5634","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"quality_controlled":"1","project":[{"call_identifier":"FWF","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","grant_number":"T 560-B17","_id":"2529486C-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation","grant_number":"I 812-B12","_id":"2527D5CC-B435-11E9-9278-68D0E5697425"}],"doi":"10.1016/j.cell.2015.01.008","acknowledged_ssus":[{"_id":"SSU"}],"language":[{"iso":"eng"}],"month":"02","_id":"1537","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","title":"Cortical contractility triggers a stochastic switch to fast amoeboid cell motility","status":"public","ddc":["570"],"intvolume":" 160","pubrep_id":"484","oa_version":"Published Version","file":[{"file_id":"5003","relation":"main_file","date_created":"2018-12-12T10:13:21Z","date_updated":"2020-07-14T12:45:01Z","checksum":"228d3edf40627d897b3875088a0ac51f","file_name":"IST-2016-484-v1+1_1-s2.0-S0092867415000094-main.pdf","access_level":"open_access","creator":"system","file_size":4362653,"content_type":"application/pdf"}],"type":"journal_article","abstract":[{"text":"3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.","lang":"eng"}],"issue":"4","publication":"Cell","citation":{"chicago":"Ruprecht, Verena, Stefan Wieser, Andrew Callan Jones, Michael Smutny, Hitoshi Morita, Keisuke Sako, Vanessa Barone, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” Cell. Cell Press, 2015. https://doi.org/10.1016/j.cell.2015.01.008.","short":"V. Ruprecht, S. Wieser, A. Callan Jones, M. Smutny, H. Morita, K. Sako, V. Barone, M. Ritsch Marte, M.K. Sixt, R. Voituriez, C.-P.J. Heisenberg, Cell 160 (2015) 673–685.","mla":"Ruprecht, Verena, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” Cell, vol. 160, no. 4, Cell Press, 2015, pp. 673–85, doi:10.1016/j.cell.2015.01.008.","apa":"Ruprecht, V., Wieser, S., Callan Jones, A., Smutny, M., Morita, H., Sako, K., … Heisenberg, C.-P. J. (2015). Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.008","ieee":"V. Ruprecht et al., “Cortical contractility triggers a stochastic switch to fast amoeboid cell motility,” Cell, vol. 160, no. 4. Cell Press, pp. 673–685, 2015.","ista":"Ruprecht V, Wieser S, Callan Jones A, Smutny M, Morita H, Sako K, Barone V, Ritsch Marte M, Sixt MK, Voituriez R, Heisenberg C-PJ. 2015. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 160(4), 673–685.","ama":"Ruprecht V, Wieser S, Callan Jones A, et al. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 2015;160(4):673-685. doi:10.1016/j.cell.2015.01.008"},"page":"673 - 685","date_published":"2015-02-12T00:00:00Z","scopus_import":1,"day":"12","has_accepted_license":"1"},{"author":[{"full_name":"Behrndt, Martin","first_name":"Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"oa_version":"None","volume":16,"date_created":"2018-12-11T11:54:37Z","date_updated":"2021-01-12T06:53:56Z","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","_id":"1900","year":"2014","intvolume":" 16","department":[{"_id":"CaHe"}],"publisher":"Nature Publishing Group","title":"Lateral junction dynamics lead the way out","status":"public","publication_status":"published","issue":"2","publist_id":"5195","abstract":[{"text":"Epithelial cell layers need to be tightly regulated to maintain their integrity and correct function. Cell integration into epithelial sheets is now shown to depend on the N-WASP-regulated stabilization of cortical F-actin, which generates distinct patterns of apical-lateral contractility at E-cadherin-based cell-cell junctions.","lang":"eng"}],"type":"journal_article","doi":"10.1038/ncb2913","date_published":"2014-01-31T00:00:00Z","language":[{"iso":"eng"}],"citation":{"chicago":"Behrndt, Martin, and Carl-Philipp J Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” Nature Cell Biology. Nature Publishing Group, 2014. https://doi.org/10.1038/ncb2913.","mla":"Behrndt, Martin, and Carl-Philipp J. Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” Nature Cell Biology, vol. 16, no. 2, Nature Publishing Group, 2014, pp. 127–29, doi:10.1038/ncb2913.","short":"M. Behrndt, C.-P.J. Heisenberg, Nature Cell Biology 16 (2014) 127–129.","ista":"Behrndt M, Heisenberg C-PJ. 2014. Lateral junction dynamics lead the way out. Nature Cell Biology. 16(2), 127–129.","ieee":"M. Behrndt and C.-P. J. Heisenberg, “Lateral junction dynamics lead the way out,” Nature Cell Biology, vol. 16, no. 2. Nature Publishing Group, pp. 127–129, 2014.","apa":"Behrndt, M., & Heisenberg, C.-P. J. (2014). Lateral junction dynamics lead the way out. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb2913","ama":"Behrndt M, Heisenberg C-PJ. Lateral junction dynamics lead the way out. Nature Cell Biology. 2014;16(2):127-129. doi:10.1038/ncb2913"},"publication":"Nature Cell Biology","page":"127 - 129","quality_controlled":"1","day":"31","month":"01","scopus_import":1},{"type":"journal_article","abstract":[{"lang":"eng","text":"We derive the equations for a thin, axisymmetric elastic shell subjected to an internal active stress giving rise to active tension and moments within the shell. We discuss the stability of a cylindrical elastic shell and its response to a localized change in internal active stress. This description is relevant to describe the cellular actomyosin cortex, a thin shell at the cell surface behaving elastically at a short timescale and subjected to active internal forces arising from myosin molecular motor activity. We show that the recent observations of cell deformation following detachment of adherent cells (Maître J-L et al 2012 Science 338 253-6) are well accounted for by this mechanical description. The actin cortex elastic and bending moduli can be obtained from a quantitative analysis of cell shapes observed in these experiments. Our approach thus provides a non-invasive, imaging-based method for the extraction of cellular physical parameters."}],"_id":"1923","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","intvolume":" 16","title":"Active elastic thin shell theory for cellular deformations","ddc":["570"],"status":"public","pubrep_id":"429","file":[{"file_size":941387,"content_type":"application/pdf","creator":"system","access_level":"open_access","file_name":"IST-2016-429-v1+1_document.pdf","checksum":"8dbe81ec656bf1264d8889bda9b2b985","date_updated":"2020-07-14T12:45:21Z","date_created":"2018-12-12T10:16:16Z","relation":"main_file","file_id":"5202"}],"oa_version":"Published Version","scopus_import":1,"has_accepted_license":"1","day":"01","citation":{"ama":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. Active elastic thin shell theory for cellular deformations. New Journal of Physics. 2014;16. doi:10.1088/1367-2630/16/6/065005","ista":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. 2014. Active elastic thin shell theory for cellular deformations. New Journal of Physics. 16, 065005.","ieee":"H. Berthoumieux, J.-L. Maître, C.-P. J. Heisenberg, E. Paluch, F. Julicher, and G. Salbreux, “Active elastic thin shell theory for cellular deformations,” New Journal of Physics, vol. 16. IOP Publishing Ltd., 2014.","apa":"Berthoumieux, H., Maître, J.-L., Heisenberg, C.-P. J., Paluch, E., Julicher, F., & Salbreux, G. (2014). Active elastic thin shell theory for cellular deformations. New Journal of Physics. IOP Publishing Ltd. https://doi.org/10.1088/1367-2630/16/6/065005","mla":"Berthoumieux, Hélène, et al. “Active Elastic Thin Shell Theory for Cellular Deformations.” New Journal of Physics, vol. 16, 065005, IOP Publishing Ltd., 2014, doi:10.1088/1367-2630/16/6/065005.","short":"H. Berthoumieux, J.-L. Maître, C.-P.J. Heisenberg, E. Paluch, F. Julicher, G. Salbreux, New Journal of Physics 16 (2014).","chicago":"Berthoumieux, Hélène, Jean-Léon Maître, Carl-Philipp J Heisenberg, Ewa Paluch, Frank Julicher, and Guillaume Salbreux. “Active Elastic Thin Shell Theory for Cellular Deformations.” New Journal of Physics. IOP Publishing Ltd., 2014. https://doi.org/10.1088/1367-2630/16/6/065005."},"publication":"New Journal of Physics","date_published":"2014-06-01T00:00:00Z","article_number":"065005","publist_id":"5171","file_date_updated":"2020-07-14T12:45:21Z","year":"2014","department":[{"_id":"CaHe"}],"publisher":"IOP Publishing Ltd.","publication_status":"published","author":[{"full_name":"Berthoumieux, Hélène","last_name":"Berthoumieux","first_name":"Hélène"},{"orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","last_name":"Maître","first_name":"Jean-Léon","full_name":"Maître, Jean-Léon"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Paluch, Ewa","first_name":"Ewa","last_name":"Paluch"},{"first_name":"Frank","last_name":"Julicher","full_name":"Julicher, Frank"},{"full_name":"Salbreux, Guillaume","last_name":"Salbreux","first_name":"Guillaume"}],"volume":16,"date_updated":"2021-01-12T06:54:06Z","date_created":"2018-12-11T11:54:44Z","month":"06","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"quality_controlled":"1","doi":"10.1088/1367-2630/16/6/065005","language":[{"iso":"eng"}]},{"author":[{"full_name":"Smutny, Michael","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5920-9090","first_name":"Michael","last_name":"Smutny"},{"full_name":"Behrndt, Martin","last_name":"Behrndt","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Campinho, Pedro","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8526-5416","first_name":"Pedro","last_name":"Campinho"},{"last_name":"Ruprecht","first_name":"Verena","orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"volume":1189,"date_created":"2019-03-26T08:55:59Z","date_updated":"2023-09-05T14:12:00Z","pmid":1,"year":"2014","publisher":"Springer","department":[{"_id":"CaHe"}],"editor":[{"full_name":"Nelson, Celeste","first_name":"Celeste","last_name":"Nelson"}],"publication_status":"published","place":"New York, NY","doi":"10.1007/978-1-4939-1164-6_15","language":[{"iso":"eng"}],"external_id":{"pmid":["25245697"]},"quality_controlled":"1","publication_identifier":{"issn":["1064-3745"],"isbn":["9781493911639","9781493911646"],"eissn":["1940-6029"]},"month":"08","oa_version":"None","_id":"6178","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","intvolume":" 1189","title":"UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo","status":"public","abstract":[{"lang":"eng","text":"Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess mechanical tension in different tissues of the developing zebrafish and in cultures of primary germ layer progenitor cells ex vivo."}],"type":"book_chapter","date_published":"2014-08-22T00:00:00Z","citation":{"chicago":"Smutny, Michael, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp J Heisenberg. “UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” In Tissue Morphogenesis, edited by Celeste Nelson, 1189:219–35. Methods in Molecular Biology. New York, NY: Springer, 2014. https://doi.org/10.1007/978-1-4939-1164-6_15.","short":"M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, C.-P.J. Heisenberg, in:, C. Nelson (Ed.), Tissue Morphogenesis, Springer, New York, NY, 2014, pp. 219–235.","mla":"Smutny, Michael, et al. “UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” Tissue Morphogenesis, edited by Celeste Nelson, vol. 1189, Springer, 2014, pp. 219–35, doi:10.1007/978-1-4939-1164-6_15.","apa":"Smutny, M., Behrndt, M., Campinho, P., Ruprecht, V., & Heisenberg, C.-P. J. (2014). UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In C. Nelson (Ed.), Tissue Morphogenesis (Vol. 1189, pp. 219–235). New York, NY: Springer. https://doi.org/10.1007/978-1-4939-1164-6_15","ieee":"M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, and C.-P. J. Heisenberg, “UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo,” in Tissue Morphogenesis, vol. 1189, C. Nelson, Ed. New York, NY: Springer, 2014, pp. 219–235.","ista":"Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. 2014.UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In: Tissue Morphogenesis. vol. 1189, 219–235.","ama":"Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In: Nelson C, ed. Tissue Morphogenesis. Vol 1189. Methods in Molecular Biology. New York, NY: Springer; 2014:219-235. doi:10.1007/978-1-4939-1164-6_15"},"publication":"Tissue Morphogenesis","page":"219-235","article_processing_charge":"No","day":"22","series_title":"Methods in Molecular Biology"},{"publisher":"Cell Press","department":[{"_id":"CaHe"}],"publication_status":"published","pmid":1,"acknowledgement":"We are grateful to members of the C.-P.H. lab, M. Concha, D. Siekhaus, and J. Vermot for comments on the manuscript and to M. Furutani-Seiki for sharing reagents. This work was supported by the Institute of Science and Technology Austria and an Alexander von Humboldt Foundation fellowship to J.C.","year":"2014","volume":31,"date_created":"2018-12-11T11:54:41Z","date_updated":"2023-09-07T12:05:08Z","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"961"}]},"author":[{"full_name":"Compagnon, Julien","last_name":"Compagnon","first_name":"Julien","id":"2E3E0988-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Barone, Vanessa","last_name":"Barone","first_name":"Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Rajshekar, Srivarsha","first_name":"Srivarsha","last_name":"Rajshekar"},{"full_name":"Kottmeier, Rita","first_name":"Rita","last_name":"Kottmeier"},{"full_name":"Pranjic-Ferscha, Kornelija","last_name":"Pranjic-Ferscha","first_name":"Kornelija","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt","first_name":"Martin"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"publist_id":"5182","quality_controlled":"1","external_id":{"pmid":["25535919"]},"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/25535919","open_access":"1"}],"oa":1,"language":[{"iso":"eng"}],"doi":"10.1016/j.devcel.2014.11.003","month":"12","intvolume":" 31","title":"The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"1912","oa_version":"Published Version","type":"journal_article","issue":"6","abstract":[{"text":"Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function.","lang":"eng"}],"page":"774 - 783","citation":{"mla":"Compagnon, Julien, et al. “The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ.” Developmental Cell, vol. 31, no. 6, Cell Press, 2014, pp. 774–83, doi:10.1016/j.devcel.2014.11.003.","short":"J. Compagnon, V. Barone, S. Rajshekar, R. Kottmeier, K. Pranjic-Ferscha, M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 31 (2014) 774–783.","chicago":"Compagnon, Julien, Vanessa Barone, Srivarsha Rajshekar, Rita Kottmeier, Kornelija Pranjic-Ferscha, Martin Behrndt, and Carl-Philipp J Heisenberg. “The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ.” Developmental Cell. Cell Press, 2014. https://doi.org/10.1016/j.devcel.2014.11.003.","ama":"Compagnon J, Barone V, Rajshekar S, et al. The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. Developmental Cell. 2014;31(6):774-783. doi:10.1016/j.devcel.2014.11.003","ista":"Compagnon J, Barone V, Rajshekar S, Kottmeier R, Pranjic-Ferscha K, Behrndt M, Heisenberg C-PJ. 2014. The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. Developmental Cell. 31(6), 774–783.","apa":"Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., & Heisenberg, C.-P. J. (2014). The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2014.11.003","ieee":"J. Compagnon et al., “The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ,” Developmental Cell, vol. 31, no. 6. Cell Press, pp. 774–783, 2014."},"publication":"Developmental Cell","date_published":"2014-12-22T00:00:00Z","scopus_import":"1","article_processing_charge":"No","day":"22"},{"page":"1405 - 1414","publication":"Nature Cell Biology","citation":{"ista":"Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. 2013. Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. Nature Cell Biology. 15, 1405–1414.","ieee":"P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, and C.-P. J. Heisenberg, “Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly,” Nature Cell Biology, vol. 15. Nature Publishing Group, pp. 1405–1414, 2013.","apa":"Campinho, P., Behrndt, M., Ranft, J., Risler, T., Minc, N., & Heisenberg, C.-P. J. (2013). Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb2869","ama":"Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. Nature Cell Biology. 2013;15:1405-1414. doi:10.1038/ncb2869","chicago":"Campinho, Pedro, Martin Behrndt, Jonas Ranft, Thomas Risler, Nicolas Minc, and Carl-Philipp J Heisenberg. “Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” Nature Cell Biology. Nature Publishing Group, 2013. https://doi.org/10.1038/ncb2869.","mla":"Campinho, Pedro, et al. “Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” Nature Cell Biology, vol. 15, Nature Publishing Group, 2013, pp. 1405–14, doi:10.1038/ncb2869.","short":"P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, C.-P.J. Heisenberg, Nature Cell Biology 15 (2013) 1405–1414."},"date_published":"2013-11-10T00:00:00Z","scopus_import":1,"day":"10","title":"Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly","status":"public","intvolume":" 15","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"2282","oa_version":"Submitted Version","type":"journal_article","abstract":[{"lang":"eng","text":"Epithelial spreading is a common and fundamental aspect of various developmental and disease-related processes such as epithelial closure and wound healing. A key challenge for epithelial tissues undergoing spreading is to increase their surface area without disrupting epithelial integrity. Here we show that orienting cell divisions by tension constitutes an efficient mechanism by which the enveloping cell layer (EVL) releases anisotropic tension while undergoing spreading during zebrafish epiboly. The control of EVL cell-division orientation by tension involves cell elongation and requires myosin II activity to align the mitotic spindle with the main tension axis. We also found that in the absence of tension-oriented cell divisions and in the presence of increased tissue tension, EVL cells undergo ectopic fusions, suggesting that the reduction of tension anisotropy by oriented cell divisions is required to prevent EVL cells from fusing. We conclude that cell-division orientation by tension constitutes a key mechanism for limiting tension anisotropy and thus promoting tissue spreading during EVL epiboly."}],"quality_controlled":"1","project":[{"_id":"252ABD0A-B435-11E9-9278-68D0E5697425","grant_number":"I 930-B20","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","call_identifier":"FWF"}],"main_file_link":[{"url":"http://hal.upmc.fr/hal-00983313/","open_access":"1"}],"oa":1,"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"language":[{"iso":"eng"}],"doi":"10.1038/ncb2869","month":"11","publication_status":"published","department":[{"_id":"CaHe"}],"publisher":"Nature Publishing Group","acknowledgement":"This work was supported by the IST Austria and MPI-CBG ","year":"2013","date_updated":"2023-02-21T17:02:44Z","date_created":"2018-12-11T11:56:45Z","volume":15,"author":[{"full_name":"Campinho, Pedro","last_name":"Campinho","first_name":"Pedro","orcid":"0000-0002-8526-5416","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt","first_name":"Martin"},{"first_name":"Jonas","last_name":"Ranft","full_name":"Ranft, Jonas"},{"last_name":"Risler","first_name":"Thomas","full_name":"Risler, Thomas"},{"last_name":"Minc","first_name":"Nicolas","full_name":"Minc, Nicolas"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"1403"}]},"publist_id":"4652"},{"day":"04","scopus_import":1,"date_published":"2013-10-04T00:00:00Z","publication":"EMBO Journal","citation":{"chicago":"Campinho, Pedro, and Carl-Philipp J Heisenberg. “The Force and Effect of Cell Proliferation.” EMBO Journal. Wiley-Blackwell, 2013. https://doi.org/10.1038/emboj.2013.225.","mla":"Campinho, Pedro, and Carl-Philipp J. Heisenberg. “The Force and Effect of Cell Proliferation.” EMBO Journal, vol. 32, no. 21, Wiley-Blackwell, 2013, pp. 2783–84, doi:10.1038/emboj.2013.225.","short":"P. Campinho, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 2783–2784.","ista":"Campinho P, Heisenberg C-PJ. 2013. The force and effect of cell proliferation. EMBO Journal. 32(21), 2783–2784.","ieee":"P. Campinho and C.-P. J. Heisenberg, “The force and effect of cell proliferation,” EMBO Journal, vol. 32, no. 21. Wiley-Blackwell, pp. 2783–2784, 2013.","apa":"Campinho, P., & Heisenberg, C.-P. J. (2013). The force and effect of cell proliferation. EMBO Journal. Wiley-Blackwell. https://doi.org/10.1038/emboj.2013.225","ama":"Campinho P, Heisenberg C-PJ. The force and effect of cell proliferation. EMBO Journal. 2013;32(21):2783-2784. doi:10.1038/emboj.2013.225"},"page":"2783 - 2784","abstract":[{"text":"The spatiotemporal control of cell divisions is a key factor in epithelial morphogenesis and patterning. Mao et al (2013) now describe how differential rates of proliferation within the Drosophila wing disc epithelium give rise to anisotropic tissue tension in peripheral/proximal regions of the disc. Such global tissue tension anisotropy in turn determines the orientation of cell divisions by controlling epithelial cell elongation.","lang":"eng"}],"issue":"21","type":"journal_article","oa_version":"Submitted Version","_id":"2286","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"The force and effect of cell proliferation","status":"public","intvolume":" 32","month":"10","doi":"10.1038/emboj.2013.225","language":[{"iso":"eng"}],"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817470/"}],"external_id":{"pmid":["24097062"]},"oa":1,"quality_controlled":"1","publist_id":"4645","author":[{"first_name":"Pedro","last_name":"Campinho","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8526-5416","full_name":"Campinho, Pedro"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"date_created":"2018-12-11T11:56:46Z","date_updated":"2021-01-12T06:56:32Z","volume":32,"year":"2013","pmid":1,"publication_status":"published","publisher":"Wiley-Blackwell","department":[{"_id":"CaHe"}]},{"doi":"10.1016/j.cub.2013.06.019","language":[{"iso":"eng"}],"external_id":{"pmid":["23885883"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"quality_controlled":"1","month":"07","author":[{"full_name":"Maître, Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474","first_name":"Jean-Léon","last_name":"Maître"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"date_updated":"2021-01-12T06:57:40Z","date_created":"2018-12-11T11:57:51Z","volume":23,"year":"2013","pmid":1,"publication_status":"published","publisher":"Cell Press","department":[{"_id":"CaHe"}],"file_date_updated":"2020-07-14T12:45:41Z","publist_id":"4433","date_published":"2013-07-22T00:00:00Z","publication":"Current Biology","citation":{"mla":"Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “Three Functions of Cadherins in Cell Adhesion.” Current Biology, vol. 23, no. 14, Cell Press, 2013, pp. R626–33, doi:10.1016/j.cub.2013.06.019.","short":"J.-L. Maître, C.-P.J. Heisenberg, Current Biology 23 (2013) R626–R633.","chicago":"Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “Three Functions of Cadherins in Cell Adhesion.” Current Biology. Cell Press, 2013. https://doi.org/10.1016/j.cub.2013.06.019.","ama":"Maître J-L, Heisenberg C-PJ. Three functions of cadherins in cell adhesion. Current Biology. 2013;23(14):R626-R633. doi:10.1016/j.cub.2013.06.019","ista":"Maître J-L, Heisenberg C-PJ. 2013. Three functions of cadherins in cell adhesion. Current Biology. 23(14), R626–R633.","ieee":"J.-L. Maître and C.-P. J. Heisenberg, “Three functions of cadherins in cell adhesion,” Current Biology, vol. 23, no. 14. Cell Press, pp. R626–R633, 2013.","apa":"Maître, J.-L., & Heisenberg, C.-P. J. (2013). Three functions of cadherins in cell adhesion. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.06.019"},"page":"R626 - R633","day":"22","has_accepted_license":"1","scopus_import":1,"oa_version":"Published Version","file":[{"file_id":"5881","relation":"main_file","date_updated":"2020-07-14T12:45:41Z","date_created":"2019-01-24T15:40:22Z","checksum":"6a424b2f007b41d4955a9135793b2162","file_name":"2013_CurrentBiology_Maitre.pdf","access_level":"open_access","creator":"dernst","content_type":"application/pdf","file_size":247320}],"_id":"2469","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Three functions of cadherins in cell adhesion","ddc":["570"],"status":"public","intvolume":" 23","abstract":[{"lang":"eng","text":"Cadherins are transmembrane proteins that mediate cell–cell adhesion in animals. By regulating contact formation and stability, cadherins play a crucial role in tissue morphogenesis and homeostasis. Here, we review the three major unctions of cadherins in cell–cell contact formation and stability. Two of those functions lead to a decrease in interfacial ension at the forming cell–cell contact, thereby promoting contact expansion — first, by providing adhesion tension that lowers interfacial tension at the cell–cell contact, and second, by signaling to the actomyosin cytoskeleton in order to reduce cortex tension and thus interfacial tension at the contact. The third function of cadherins in cell–cell contact formation is to stabilize the contact by resisting mechanical forces that pull on the contact."}],"issue":"14","type":"journal_article"},{"year":"2013","_id":"2833","acknowledgement":"C.-P.H. is supported by the Institute of Science and Technology Austria and grants from the Deutsche Forschungsgemeinschaft (DFG) and Fonds zur Förderung der wissenschaftlichen Forschung (FWF).","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","title":"Forces in tissue morphogenesis and patterning","status":"public","intvolume":" 153","department":[{"_id":"CaHe"}],"publisher":"Cell Press","author":[{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Yohanns","last_name":"Bellaïche","full_name":"Bellaïche, Yohanns"}],"date_created":"2018-12-11T11:59:50Z","date_updated":"2021-01-12T07:00:04Z","volume":153,"oa_version":"None","type":"journal_article","abstract":[{"lang":"eng","text":"During development, mechanical forces cause changes in size, shape, number, position, and gene expression of cells. They are therefore integral to any morphogenetic processes. Force generation by actin-myosin networks and force transmission through adhesive complexes are two self-organizing phenomena driving tissue morphogenesis. Coordination and integration of forces by long-range force transmission and mechanosensing of cells within tissues produce large-scale tissue shape changes. Extrinsic mechanical forces also control tissue patterning by modulating cell fate specification and differentiation. Thus, the interplay between tissue mechanics and biochemical signaling orchestrates tissue morphogenesis and patterning in development."}],"issue":"5","publist_id":"3966","publication":"Cell","citation":{"ama":"Heisenberg C-PJ, Bellaïche Y. Forces in tissue morphogenesis and patterning. Cell. 2013;153(5):948-962. doi:10.1016/j.cell.2013.05.008","apa":"Heisenberg, C.-P. J., & Bellaïche, Y. (2013). Forces in tissue morphogenesis and patterning. Cell. Cell Press. https://doi.org/10.1016/j.cell.2013.05.008","ieee":"C.-P. J. Heisenberg and Y. Bellaïche, “Forces in tissue morphogenesis and patterning,” Cell, vol. 153, no. 5. Cell Press, pp. 948–962, 2013.","ista":"Heisenberg C-PJ, Bellaïche Y. 2013. Forces in tissue morphogenesis and patterning. Cell. 153(5), 948–962.","short":"C.-P.J. Heisenberg, Y. Bellaïche, Cell 153 (2013) 948–962.","mla":"Heisenberg, Carl-Philipp J., and Yohanns Bellaïche. “Forces in Tissue Morphogenesis and Patterning.” Cell, vol. 153, no. 5, Cell Press, 2013, pp. 948–62, doi:10.1016/j.cell.2013.05.008.","chicago":"Heisenberg, Carl-Philipp J, and Yohanns Bellaïche. “Forces in Tissue Morphogenesis and Patterning.” Cell. Cell Press, 2013. https://doi.org/10.1016/j.cell.2013.05.008."},"quality_controlled":"1","page":"948 - 962","date_published":"2013-05-23T00:00:00Z","doi":"10.1016/j.cell.2013.05.008","language":[{"iso":"eng"}],"scopus_import":1,"day":"23","month":"05"},{"issue":"6","publist_id":"3956","abstract":[{"lang":"eng","text":"In zebrafish early development, blastoderm cells undergo extensive radial intercalations, triggering the spreading of the blastoderm over the yolk cell and thereby initiating embryonic body axis formation. Now reporting in Developmental Cell, Song et al. (2013) demonstrate a critical function for EGF-dependent E-cadherin endocytosis in promoting blastoderm cell intercalations."}],"type":"journal_article","author":[{"last_name":"Morita","first_name":"Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","full_name":"Morita, Hitoshi"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"volume":24,"oa_version":"None","date_updated":"2021-01-12T07:00:09Z","date_created":"2018-12-11T11:59:52Z","_id":"2841","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2013","intvolume":" 24","publisher":"Cell Press","department":[{"_id":"CaHe"}],"title":"Holding on and letting go: Cadherin turnover in cell intercalation","publication_status":"published","status":"public","day":"25","month":"05","scopus_import":1,"doi":"10.1016/j.devcel.2013.03.007","date_published":"2013-05-25T00:00:00Z","language":[{"iso":"eng"}],"citation":{"short":"H. Morita, C.-P.J. Heisenberg, Developmental Cell 24 (2013) 567–569.","mla":"Morita, Hitoshi, and Carl-Philipp J. Heisenberg. “Holding on and Letting Go: Cadherin Turnover in Cell Intercalation.” Developmental Cell, vol. 24, no. 6, Cell Press, 2013, pp. 567–69, doi:10.1016/j.devcel.2013.03.007.","chicago":"Morita, Hitoshi, and Carl-Philipp J Heisenberg. “Holding on and Letting Go: Cadherin Turnover in Cell Intercalation.” Developmental Cell. Cell Press, 2013. https://doi.org/10.1016/j.devcel.2013.03.007.","ama":"Morita H, Heisenberg C-PJ. Holding on and letting go: Cadherin turnover in cell intercalation. Developmental Cell. 2013;24(6):567-569. doi:10.1016/j.devcel.2013.03.007","ieee":"H. Morita and C.-P. J. Heisenberg, “Holding on and letting go: Cadherin turnover in cell intercalation,” Developmental Cell, vol. 24, no. 6. Cell Press, pp. 567–569, 2013.","apa":"Morita, H., & Heisenberg, C.-P. J. (2013). Holding on and letting go: Cadherin turnover in cell intercalation. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2013.03.007","ista":"Morita H, Heisenberg C-PJ. 2013. Holding on and letting go: Cadherin turnover in cell intercalation. Developmental Cell. 24(6), 567–569."},"publication":"Developmental Cell","page":"567 - 569","quality_controlled":"1"},{"publisher":"Company of Biologists","department":[{"_id":"CaHe"}],"publication_status":"published","pmid":1,"year":"2013","acknowledgement":"Deposited in PMC for release after 12 months. We thank members of the Amack lab for helpful discussions and Mahendra Sonawane for donating reagents.","volume":140,"date_updated":"2021-01-12T07:00:20Z","date_created":"2018-12-11T11:59:59Z","author":[{"last_name":"Tay","first_name":"Hwee","full_name":"Tay, Hwee"},{"last_name":"Schulze","first_name":"Sabrina","full_name":"Schulze, Sabrina"},{"id":"2E3E0988-F248-11E8-B48F-1D18A9856A87","last_name":"Compagnon","first_name":"Julien","full_name":"Compagnon, Julien"},{"full_name":"Foley, Fiona","last_name":"Foley","first_name":"Fiona"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Yost, H Joseph","last_name":"Yost","first_name":"H Joseph"},{"full_name":"Abdelilah Seyfried, Salim","last_name":"Abdelilah Seyfried","first_name":"Salim"},{"last_name":"Amack","first_name":"Jeffrey","full_name":"Amack, Jeffrey"}],"publist_id":"3927","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596994/"}],"oa":1,"external_id":{"pmid":["23482490"]},"language":[{"iso":"eng"}],"doi":"10.1242/dev.087130","month":"04","intvolume":" 140","title":"Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle","status":"public","_id":"2862","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Submitted Version","type":"journal_article","issue":"7","abstract":[{"text":"Motile cilia perform crucial functions during embryonic development and throughout adult life. Development of organs containing motile cilia involves regulation of cilia formation (ciliogenesis) and formation of a luminal space (lumenogenesis) in which cilia generate fluid flows. Control of ciliogenesis and lumenogenesis is not yet fully understood, and it remains unclear whether these processes are coupled. In the zebrafish embryo, lethal giant larvae 2 (lgl2) is expressed prominently in ciliated organs. Lgl proteins are involved in establishing cell polarity and have been implicated in vesicle trafficking. Here, we identified a role for Lgl2 in development of ciliated epithelia in Kupffer's vesicle, which directs left-right asymmetry of the embryo; the otic vesicles, which give rise to the inner ear; and the pronephric ducts of the kidney. Using Kupffer's vesicle as a model ciliated organ, we found that depletion of Lgl2 disrupted lumen formation and reduced cilia number and length. Immunofluorescence and time-lapse imaging of Kupffer's vesicle morphogenesis in Lgl2-deficient embryos suggested cell adhesion defects and revealed loss of the adherens junction component E-cadherin at lateral membranes. Genetic interaction experiments indicate that Lgl2 interacts with Rab11a to regulate E-cadherin and mediate lumen formation that is uncoupled from cilia formation. These results uncover new roles and interactions for Lgl2 that are crucial for both lumenogenesis and ciliogenesis and indicate that these processes are genetically separable in zebrafish.","lang":"eng"}],"page":"1550 - 1559","citation":{"short":"H. Tay, S. Schulze, J. Compagnon, F. Foley, C.-P.J. Heisenberg, H.J. Yost, S. Abdelilah Seyfried, J. Amack, Development 140 (2013) 1550–1559.","mla":"Tay, Hwee, et al. “Lethal Giant Larvae 2 Regulates Development of the Ciliated Organ Kupffer’s Vesicle.” Development, vol. 140, no. 7, Company of Biologists, 2013, pp. 1550–59, doi:10.1242/dev.087130.","chicago":"Tay, Hwee, Sabrina Schulze, Julien Compagnon, Fiona Foley, Carl-Philipp J Heisenberg, H Joseph Yost, Salim Abdelilah Seyfried, and Jeffrey Amack. “Lethal Giant Larvae 2 Regulates Development of the Ciliated Organ Kupffer’s Vesicle.” Development. Company of Biologists, 2013. https://doi.org/10.1242/dev.087130.","ama":"Tay H, Schulze S, Compagnon J, et al. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. Development. 2013;140(7):1550-1559. doi:10.1242/dev.087130","apa":"Tay, H., Schulze, S., Compagnon, J., Foley, F., Heisenberg, C.-P. J., Yost, H. J., … Amack, J. (2013). Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. Development. Company of Biologists. https://doi.org/10.1242/dev.087130","ieee":"H. Tay et al., “Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle,” Development, vol. 140, no. 7. Company of Biologists, pp. 1550–1559, 2013.","ista":"Tay H, Schulze S, Compagnon J, Foley F, Heisenberg C-PJ, Yost HJ, Abdelilah Seyfried S, Amack J. 2013. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. Development. 140(7), 1550–1559."},"publication":"Development","date_published":"2013-04-01T00:00:00Z","scopus_import":1,"day":"01"},{"scopus_import":1,"month":"02","day":"01","project":[{"grant_number":"HE_3231/6-1","_id":"252064B8-B435-11E9-9278-68D0E5697425","name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo"},{"grant_number":"I 812-B12","_id":"2527D5CC-B435-11E9-9278-68D0E5697425","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation","call_identifier":"FWF"}],"page":"147 - 150","quality_controlled":"1","citation":{"mla":"Maître, Jean-Léon, et al. “Cell Adhesion Mechanics of Zebrafish Gastrulation.” Medecine Sciences, vol. 29, no. 2, Éditions Médicales et Scientifiques, 2013, pp. 147–50, doi:10.1051/medsci/2013292011.","short":"J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch, C.-P.J. Heisenberg, Medecine Sciences 29 (2013) 147–150.","chicago":"Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux, Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Cell Adhesion Mechanics of Zebrafish Gastrulation.” Medecine Sciences. Éditions Médicales et Scientifiques, 2013. https://doi.org/10.1051/medsci/2013292011.","ama":"Maître J-L, Berthoumieux H, Krens G, et al. Cell adhesion mechanics of zebrafish gastrulation. Medecine Sciences. 2013;29(2):147-150. doi:10.1051/medsci/2013292011","ista":"Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg C-PJ. 2013. Cell adhesion mechanics of zebrafish gastrulation. Medecine Sciences. 29(2), 147–150.","apa":"Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch, E., & Heisenberg, C.-P. J. (2013). Cell adhesion mechanics of zebrafish gastrulation. Medecine Sciences. Éditions Médicales et Scientifiques. https://doi.org/10.1051/medsci/2013292011","ieee":"J.-L. Maître et al., “Cell adhesion mechanics of zebrafish gastrulation,” Medecine Sciences, vol. 29, no. 2. Éditions Médicales et Scientifiques, pp. 147–150, 2013."},"publication":"Medecine Sciences","language":[{"iso":"eng"}],"date_published":"2013-02-01T00:00:00Z","doi":"10.1051/medsci/2013292011","type":"journal_article","issue":"2","publist_id":"3877","publisher":"Éditions Médicales et Scientifiques","department":[{"_id":"CaHe"}],"intvolume":" 29","publication_status":"published","status":"public","title":"Cell adhesion mechanics of zebrafish gastrulation","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"2884","year":"2013","oa_version":"None","volume":29,"date_updated":"2021-01-12T07:00:28Z","date_created":"2018-12-11T12:00:08Z","author":[{"first_name":"Jean-Léon","last_name":"Maître","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474","full_name":"Maître, Jean-Léon"},{"first_name":"Hélène","last_name":"Berthoumieux","full_name":"Berthoumieux, Hélène"},{"first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel"},{"last_name":"Salbreux","first_name":"Guillaume","full_name":"Salbreux, Guillaume"},{"last_name":"Julicher","first_name":"Frank","full_name":"Julicher, Frank"},{"last_name":"Paluch","first_name":"Ewa","full_name":"Paluch, Ewa"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}]},{"acknowledgement":"This work was supported by the SNSF, the Swiss SystemsX.ch initiative and LipidX-2008/011 (M.G-G. and F.G.v.d.G.), by the Fondation SANTE-Vaduz/Aide au Soutien des Nouvelles Thérapies (F.G.v.d.G.) and by the ERC, the NCCR Frontiers in Genetics and Chemical Biology programmes and the Polish–Swiss research program (M.G-G.).","_id":"2918","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","year":"2013","publisher":"Nature Publishing Group","department":[{"_id":"CaHe"}],"intvolume":" 15","publication_status":"published","status":"public","title":"Anthrax toxin receptor 2a controls mitotic spindle positioning","author":[{"full_name":"Castanon, Irinka","first_name":"Irinka","last_name":"Castanon"},{"full_name":"Abrami, Laurence","last_name":"Abrami","first_name":"Laurence"},{"last_name":"Holtzer","first_name":"Laurent","full_name":"Holtzer, Laurent"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Van Der Goot, Françoise","last_name":"Van Der Goot","first_name":"Françoise"},{"full_name":"González Gaitán, Marcos","first_name":"Marcos","last_name":"González Gaitán"}],"oa_version":"None","volume":15,"date_created":"2018-12-11T12:00:20Z","date_updated":"2021-01-12T07:00:41Z","type":"journal_article","issue":"1","publist_id":"3819","abstract":[{"text":"Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.\r\n","lang":"eng"}],"citation":{"ama":"Castanon I, Abrami L, Holtzer L, Heisenberg C-PJ, Van Der Goot F, González Gaitán M. Anthrax toxin receptor 2a controls mitotic spindle positioning. Nature Cell Biology. 2013;15(1):28-39. doi:10.1038/ncb2632","ieee":"I. Castanon, L. Abrami, L. Holtzer, C.-P. J. Heisenberg, F. Van Der Goot, and M. González Gaitán, “Anthrax toxin receptor 2a controls mitotic spindle positioning,” Nature Cell Biology, vol. 15, no. 1. Nature Publishing Group, pp. 28–39, 2013.","apa":"Castanon, I., Abrami, L., Holtzer, L., Heisenberg, C.-P. J., Van Der Goot, F., & González Gaitán, M. (2013). Anthrax toxin receptor 2a controls mitotic spindle positioning. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb2632","ista":"Castanon I, Abrami L, Holtzer L, Heisenberg C-PJ, Van Der Goot F, González Gaitán M. 2013. Anthrax toxin receptor 2a controls mitotic spindle positioning. Nature Cell Biology. 15(1), 28–39.","short":"I. Castanon, L. Abrami, L. Holtzer, C.-P.J. Heisenberg, F. Van Der Goot, M. González Gaitán, Nature Cell Biology 15 (2013) 28–39.","mla":"Castanon, Irinka, et al. “Anthrax Toxin Receptor 2a Controls Mitotic Spindle Positioning.” Nature Cell Biology, vol. 15, no. 1, Nature Publishing Group, 2013, pp. 28–39, doi:10.1038/ncb2632.","chicago":"Castanon, Irinka, Laurence Abrami, Laurent Holtzer, Carl-Philipp J Heisenberg, Françoise Van Der Goot, and Marcos González Gaitán. “Anthrax Toxin Receptor 2a Controls Mitotic Spindle Positioning.” Nature Cell Biology. Nature Publishing Group, 2013. https://doi.org/10.1038/ncb2632."},"publication":"Nature Cell Biology","page":"28 - 39","quality_controlled":"1","doi":"10.1038/ncb2632","date_published":"2013-01-01T00:00:00Z","language":[{"iso":"eng"}],"scopus_import":1,"day":"01","month":"01"},{"citation":{"chicago":"Compagnon, Julien, and Carl-Philipp J Heisenberg. “Neurulation Coordinating Cell Polarisation and Lumen Formation.” EMBO Journal. Wiley-Blackwell, 2013. https://doi.org/10.1038/emboj.2012.325.","short":"J. Compagnon, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 1–3.","mla":"Compagnon, Julien, and Carl-Philipp J. Heisenberg. “Neurulation Coordinating Cell Polarisation and Lumen Formation.” EMBO Journal, vol. 32, no. 1, Wiley-Blackwell, 2013, pp. 1–3, doi:10.1038/emboj.2012.325.","apa":"Compagnon, J., & Heisenberg, C.-P. J. (2013). Neurulation coordinating cell polarisation and lumen formation. EMBO Journal. Wiley-Blackwell. https://doi.org/10.1038/emboj.2012.325","ieee":"J. Compagnon and C.-P. J. Heisenberg, “Neurulation coordinating cell polarisation and lumen formation,” EMBO Journal, vol. 32, no. 1. Wiley-Blackwell, pp. 1–3, 2013.","ista":"Compagnon J, Heisenberg C-PJ. 2013. Neurulation coordinating cell polarisation and lumen formation. EMBO Journal. 32(1), 1–3.","ama":"Compagnon J, Heisenberg C-PJ. Neurulation coordinating cell polarisation and lumen formation. EMBO Journal. 2013;32(1):1-3. doi:10.1038/emboj.2012.325"},"publication":"EMBO Journal","page":"1 - 3","date_published":"2013-01-09T00:00:00Z","scopus_import":1,"day":"09","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"2920","intvolume":" 32","title":"Neurulation coordinating cell polarisation and lumen formation","status":"public","oa_version":"Submitted Version","type":"journal_article","issue":"1","abstract":[{"lang":"eng","text":"Cell polarisation in development is a common and fundamental process underlying embryo patterning and morphogenesis, and has been extensively studied over the past years. Our current knowledge of cell polarisation in development is predominantly based on studies that have analysed polarisation of single cells, such as eggs, or cellular aggregates with a stable polarising interface, such as cultured epithelial cells (St Johnston and Ahringer, 2010). However, in embryonic development, particularly of vertebrates, cell polarisation processes often encompass large numbers of cells that are placed within moving and proliferating tissues, and undergo mesenchymal-to-epithelial transitions with a highly complex spatiotemporal choreography. How such intricate cell polarisation processes in embryonic development are achieved has only started to be analysed. By using live imaging of neurulation in the transparent zebrafish embryo, Buckley et al (2012) now describe a novel polarisation strategy by which cells assemble an apical domain in the part of their cell body that intersects with the midline of the forming neural rod. This mechanism, along with the previously described mirror-symmetric divisions (Tawk et al, 2007), is thought to trigger formation of both neural rod midline and lumen."}],"external_id":{"pmid":["23211745"]},"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545307/"}],"oa":1,"quality_controlled":"1","doi":"10.1038/emboj.2012.325","language":[{"iso":"eng"}],"month":"01","pmid":1,"year":"2013","publisher":"Wiley-Blackwell","department":[{"_id":"CaHe"}],"publication_status":"published","author":[{"full_name":"Compagnon, Julien","id":"2E3E0988-F248-11E8-B48F-1D18A9856A87","last_name":"Compagnon","first_name":"Julien"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"volume":32,"date_created":"2018-12-11T12:00:20Z","date_updated":"2021-01-12T07:00:42Z","publist_id":"3817"},{"month":"10","doi":"10.1126/science.1224143","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"SSU"}],"project":[{"call_identifier":"FWF","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","grant_number":"I 930-B20","_id":"252ABD0A-B435-11E9-9278-68D0E5697425"}],"quality_controlled":"1","publist_id":"3778","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"1403"}]},"author":[{"full_name":"Behrndt, Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt","first_name":"Martin"},{"last_name":"Salbreux","first_name":"Guillaume","full_name":"Salbreux, Guillaume"},{"full_name":"Campinho, Pedro","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8526-5416","first_name":"Pedro","last_name":"Campinho"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert"},{"full_name":"Oswald, Felix","first_name":"Felix","last_name":"Oswald"},{"full_name":"Roensch, Julia","id":"4220E59C-F248-11E8-B48F-1D18A9856A87","first_name":"Julia","last_name":"Roensch"},{"full_name":"Grill, Stephan","first_name":"Stephan","last_name":"Grill"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"volume":338,"date_created":"2018-12-11T12:00:30Z","date_updated":"2023-02-21T17:02:44Z","year":"2012","department":[{"_id":"CaHe"},{"_id":"Bio"}],"publisher":"American Association for the Advancement of Science","publication_status":"published","day":"12","scopus_import":1,"date_published":"2012-10-12T00:00:00Z","citation":{"chicago":"Behrndt, Martin, Guillaume Salbreux, Pedro Campinho, Robert Hauschild, Felix Oswald, Julia Roensch, Stephan Grill, and Carl-Philipp J Heisenberg. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.” Science. American Association for the Advancement of Science, 2012. https://doi.org/10.1126/science.1224143.","mla":"Behrndt, Martin, et al. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.” Science, vol. 338, no. 6104, American Association for the Advancement of Science, 2012, pp. 257–60, doi:10.1126/science.1224143.","short":"M. Behrndt, G. Salbreux, P. Campinho, R. Hauschild, F. Oswald, J. Roensch, S. Grill, C.-P.J. Heisenberg, Science 338 (2012) 257–260.","ista":"Behrndt M, Salbreux G, Campinho P, Hauschild R, Oswald F, Roensch J, Grill S, Heisenberg C-PJ. 2012. Forces driving epithelial spreading in zebrafish gastrulation. Science. 338(6104), 257–260.","ieee":"M. Behrndt et al., “Forces driving epithelial spreading in zebrafish gastrulation,” Science, vol. 338, no. 6104. American Association for the Advancement of Science, pp. 257–260, 2012.","apa":"Behrndt, M., Salbreux, G., Campinho, P., Hauschild, R., Oswald, F., Roensch, J., … Heisenberg, C.-P. J. (2012). Forces driving epithelial spreading in zebrafish gastrulation. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1224143","ama":"Behrndt M, Salbreux G, Campinho P, et al. Forces driving epithelial spreading in zebrafish gastrulation. Science. 2012;338(6104):257-260. doi:10.1126/science.1224143"},"publication":"Science","page":"257 - 260","issue":"6104","abstract":[{"text":"Contractile actomyosin rings drive various fundamental morphogenetic processes ranging from cytokinesis to wound healing. Actomyosin rings are generally thought to function by circumferential contraction. Here, we show that the spreading of the enveloping cell layer (EVL) over the yolk cell during zebrafish gastrulation is driven by a contractile actomyosin ring. In contrast to previous suggestions, we find that this ring functions not only by circumferential contraction but also by a flow-friction mechanism. This generates a pulling force through resistance against retrograde actomyosin flow. EVL spreading proceeds normally in situations where circumferential contraction is unproductive, indicating that the flow-friction mechanism is sufficient. Thus, actomyosin rings can function in epithelial morphogenesis through a combination of cable-constriction and flow-friction mechanisms.","lang":"eng"}],"type":"journal_article","oa_version":"None","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","_id":"2950","intvolume":" 338","title":"Forces driving epithelial spreading in zebrafish gastrulation","status":"public"},{"volume":338,"oa_version":"None","date_created":"2018-12-11T12:00:31Z","date_updated":"2021-01-12T07:40:00Z","author":[{"orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","last_name":"Maître","first_name":"Jean-Léon","full_name":"Maître, Jean-Léon"},{"first_name":"Hélène","last_name":"Berthoumieux","full_name":"Berthoumieux, Hélène"},{"full_name":"Krens, Gabriel","last_name":"Krens","first_name":"Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Salbreux, Guillaume","first_name":"Guillaume","last_name":"Salbreux"},{"full_name":"Julicher, Frank","first_name":"Frank","last_name":"Julicher"},{"full_name":"Paluch, Ewa","first_name":"Ewa","last_name":"Paluch"},{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"department":[{"_id":"CaHe"}],"publisher":"American Association for the Advancement of Science","intvolume":" 338","publication_status":"published","status":"public","title":"Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells","_id":"2951","year":"2012","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publist_id":"3777","issue":"6104","abstract":[{"lang":"eng","text":"Differential cell adhesion and cortex tension are thought to drive cell sorting by controlling cell-cell contact formation. Here, we show that cell adhesion and cortex tension have different mechanical functions in controlling progenitor cell-cell contact formation and sorting during zebrafish gastrulation. Cortex tension controls cell-cell contact expansion by modulating interfacial tension at the contact. By contrast, adhesion has little direct function in contact expansion, but instead is needed to mechanically couple the cortices of adhering cells at their contacts, allowing cortex tension to control contact expansion. The coupling function of adhesion is mediated by E-cadherin and limited by the mechanical anchoring of E-cadherin to the cortex. Thus, cell adhesion provides the mechanical scaffold for cell cortex tension to drive cell sorting during gastrulation."}],"type":"journal_article","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"SSU"}],"doi":"10.1126/science.1225399","date_published":"2012-10-12T00:00:00Z","page":"253 - 256","quality_controlled":"1","citation":{"ieee":"J.-L. Maître et al., “Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells,” Science, vol. 338, no. 6104. American Association for the Advancement of Science, pp. 253–256, 2012.","apa":"Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch, E., & Heisenberg, C.-P. J. (2012). Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1225399","ista":"Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg C-PJ. 2012. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. Science. 338(6104), 253–256.","ama":"Maître J-L, Berthoumieux H, Krens G, et al. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. Science. 2012;338(6104):253-256. doi:10.1126/science.1225399","chicago":"Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux, Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” Science. American Association for the Advancement of Science, 2012. https://doi.org/10.1126/science.1225399.","short":"J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch, C.-P.J. Heisenberg, Science 338 (2012) 253–256.","mla":"Maître, Jean-Léon, et al. “Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells.” Science, vol. 338, no. 6104, American Association for the Advancement of Science, 2012, pp. 253–56, doi:10.1126/science.1225399."},"publication":"Science","day":"12","month":"10","scopus_import":1},{"abstract":[{"lang":"eng","text":"Body axis elongation represents a common and fundamental morphogenetic process in development. A key mechanism triggering body axis elongation without additional growth is convergent extension (CE), whereby a tissue undergoes simultaneous narrowing and extension. Both collective cell migration and cell intercalation are thought to drive CE and are used to different degrees in various species as they elongate their body axis. Here, we provide an overview of CE as a general strategy for body axis elongation and discuss conserved and divergent mechanisms underlying CE among different species."}],"publist_id":"3776","issue":"21","type":"journal_article","date_updated":"2021-01-12T07:40:00Z","date_created":"2018-12-11T12:00:31Z","volume":139,"oa_version":"None","author":[{"full_name":"Tada, Masazumi","last_name":"Tada","first_name":"Masazumi"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"title":"Convergent extension Using collective cell migration and cell intercalation to shape embryos","publication_status":"published","status":"public","intvolume":" 139","department":[{"_id":"CaHe"}],"publisher":"Company of Biologists","_id":"2952","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","year":"2012","acknowledgement":"M.T. is supported by the UK Medical Research Council (MRC) and Royal Society and C.-P.H. by the Fonds zur Förderung der wissenschaftlichen Forschung (FWF), Deutsche Forschungsgemeinschaft (DFG) and Institute of Science and Technology Austria. ","day":"01","month":"11","scopus_import":1,"language":[{"iso":"eng"}],"date_published":"2012-11-01T00:00:00Z","doi":"10.1242/dev.073007","quality_controlled":"1","page":"3897 - 3904","publication":"Development","citation":{"short":"M. Tada, C.-P.J. Heisenberg, Development 139 (2012) 3897–3904.","mla":"Tada, Masazumi, and Carl-Philipp J. Heisenberg. “Convergent Extension Using Collective Cell Migration and Cell Intercalation to Shape Embryos.” Development, vol. 139, no. 21, Company of Biologists, 2012, pp. 3897–904, doi:10.1242/dev.073007.","chicago":"Tada, Masazumi, and Carl-Philipp J Heisenberg. “Convergent Extension Using Collective Cell Migration and Cell Intercalation to Shape Embryos.” Development. Company of Biologists, 2012. https://doi.org/10.1242/dev.073007.","ama":"Tada M, Heisenberg C-PJ. Convergent extension Using collective cell migration and cell intercalation to shape embryos. Development. 2012;139(21):3897-3904. doi:10.1242/dev.073007","apa":"Tada, M., & Heisenberg, C.-P. J. (2012). Convergent extension Using collective cell migration and cell intercalation to shape embryos. Development. Company of Biologists. https://doi.org/10.1242/dev.073007","ieee":"M. Tada and C.-P. J. Heisenberg, “Convergent extension Using collective cell migration and cell intercalation to shape embryos,” Development, vol. 139, no. 21. Company of Biologists, pp. 3897–3904, 2012.","ista":"Tada M, Heisenberg C-PJ. 2012. Convergent extension Using collective cell migration and cell intercalation to shape embryos. Development. 139(21), 3897–3904."}},{"day":"01","month":"10","scopus_import":1,"language":[{"iso":"eng"}],"doi":"10.1016/j.ceb.2012.09.002","date_published":"2012-10-01T00:00:00Z","quality_controlled":"1","page":"559 - 561","publication":"Current Opinion in Cell Biology","citation":{"ama":"Heisenberg C-PJ, Fässler R. Cell-cell adhesion and extracellular matrix diversity counts. Current Opinion in Cell Biology. 2012;24(5):559-561. doi:10.1016/j.ceb.2012.09.002","apa":"Heisenberg, C.-P. J., & Fässler, R. (2012). Cell-cell adhesion and extracellular matrix diversity counts. Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2012.09.002","ieee":"C.-P. J. Heisenberg and R. Fässler, “Cell-cell adhesion and extracellular matrix diversity counts,” Current Opinion in Cell Biology, vol. 24, no. 5. Elsevier, pp. 559–561, 2012.","ista":"Heisenberg C-PJ, Fässler R. 2012. Cell-cell adhesion and extracellular matrix diversity counts. Current Opinion in Cell Biology. 24(5), 559–561.","short":"C.-P.J. Heisenberg, R. Fässler, Current Opinion in Cell Biology 24 (2012) 559–561.","mla":"Heisenberg, Carl-Philipp J., and Reinhard Fässler. “Cell-Cell Adhesion and Extracellular Matrix Diversity Counts.” Current Opinion in Cell Biology, vol. 24, no. 5, Elsevier, 2012, pp. 559–61, doi:10.1016/j.ceb.2012.09.002.","chicago":"Heisenberg, Carl-Philipp J, and Reinhard Fässler. “Cell-Cell Adhesion and Extracellular Matrix Diversity Counts.” Current Opinion in Cell Biology. Elsevier, 2012. https://doi.org/10.1016/j.ceb.2012.09.002."},"publist_id":"3773","issue":"5","type":"journal_article","date_created":"2018-12-11T12:00:31Z","date_updated":"2021-01-12T07:40:01Z","volume":24,"oa_version":"None","author":[{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Fässler, Reinhard","first_name":"Reinhard","last_name":"Fässler"}],"publication_status":"published","title":"Cell-cell adhesion and extracellular matrix diversity counts","status":"public","publisher":"Elsevier","intvolume":" 24","department":[{"_id":"CaHe"}],"year":"2012","_id":"2953","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87"},{"type":"journal_article","abstract":[{"text":"How cells orchestrate their behavior during collective migration is a long-standing question. Using magnetic tweezers to apply mechanical stimuli to Xenopus mesendoderm cells, Weber etal. (2012) now reveal, in this issue of Developmental Cell, a cadherin-mediated mechanosensitive response that promotes cell polarization and movement persistence during the collective mesendoderm migration in gastrulation.","lang":"eng"}],"issue":"1","publist_id":"3426","publication_status":"published","status":"public","title":"Spurred by resistance mechanosensation in collective migration","publisher":"Cell Press","department":[{"_id":"CaHe"}],"intvolume":" 22","_id":"3245","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","year":"2012","date_created":"2018-12-11T12:02:14Z","date_updated":"2021-01-12T07:42:05Z","oa_version":"None","volume":22,"author":[{"full_name":"Behrndt, Martin","last_name":"Behrndt","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"scopus_import":1,"month":"01","day":"17","quality_controlled":"1","page":"3 - 4","publication":"Developmental Cell","citation":{"chicago":"Behrndt, Martin, and Carl-Philipp J Heisenberg. “Spurred by Resistance Mechanosensation in Collective Migration.” Developmental Cell. Cell Press, 2012. https://doi.org/10.1016/j.devcel.2011.12.018.","short":"M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 22 (2012) 3–4.","mla":"Behrndt, Martin, and Carl-Philipp J. Heisenberg. “Spurred by Resistance Mechanosensation in Collective Migration.” Developmental Cell, vol. 22, no. 1, Cell Press, 2012, pp. 3–4, doi:10.1016/j.devcel.2011.12.018.","apa":"Behrndt, M., & Heisenberg, C.-P. J. (2012). Spurred by resistance mechanosensation in collective migration. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2011.12.018","ieee":"M. Behrndt and C.-P. J. Heisenberg, “Spurred by resistance mechanosensation in collective migration,” Developmental Cell, vol. 22, no. 1. Cell Press, pp. 3–4, 2012.","ista":"Behrndt M, Heisenberg C-PJ. 2012. Spurred by resistance mechanosensation in collective migration. Developmental Cell. 22(1), 3–4.","ama":"Behrndt M, Heisenberg C-PJ. Spurred by resistance mechanosensation in collective migration. Developmental Cell. 2012;22(1):3-4. doi:10.1016/j.devcel.2011.12.018"},"language":[{"iso":"eng"}],"doi":"10.1016/j.devcel.2011.12.018","date_published":"2012-01-17T00:00:00Z"},{"scopus_import":1,"day":"01","month":"02","quality_controlled":"1","page":"148 - 153","publication":"Current Opinion in Cell Biology","citation":{"mla":"Barone, Vanessa, and Carl-Philipp J. Heisenberg. “Cell Adhesion in Embryo Morphogenesis.” Current Opinion in Cell Biology, vol. 24, no. 1, Elsevier, 2012, pp. 148–53, doi:10.1016/j.ceb.2011.11.006.","short":"V. Barone, C.-P.J. Heisenberg, Current Opinion in Cell Biology 24 (2012) 148–153.","chicago":"Barone, Vanessa, and Carl-Philipp J Heisenberg. “Cell Adhesion in Embryo Morphogenesis.” Current Opinion in Cell Biology. Elsevier, 2012. https://doi.org/10.1016/j.ceb.2011.11.006.","ama":"Barone V, Heisenberg C-PJ. Cell adhesion in embryo morphogenesis. Current Opinion in Cell Biology. 2012;24(1):148-153. doi:10.1016/j.ceb.2011.11.006","ista":"Barone V, Heisenberg C-PJ. 2012. Cell adhesion in embryo morphogenesis. Current Opinion in Cell Biology. 24(1), 148–153.","ieee":"V. Barone and C.-P. J. Heisenberg, “Cell adhesion in embryo morphogenesis,” Current Opinion in Cell Biology, vol. 24, no. 1. Elsevier, pp. 148–153, 2012.","apa":"Barone, V., & Heisenberg, C.-P. J. (2012). Cell adhesion in embryo morphogenesis. Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2011.11.006"},"language":[{"iso":"eng"}],"doi":"10.1016/j.ceb.2011.11.006","date_published":"2012-02-01T00:00:00Z","type":"journal_article","abstract":[{"text":"Visualizing and analyzing shape changes at various scales, ranging from single molecules to whole organisms, are essential for understanding complex morphogenetic processes, such as early embryonic development. Embryo morphogenesis relies on the interplay between different tissues, the properties of which are again determined by the interaction between their constituent cells. Cell interactions, on the other hand, are controlled by various molecules, such as signaling and adhesion molecules, which in order to exert their functions need to be spatiotemporally organized within and between the interacting cells. In this review, we will focus on the role of cell adhesion functioning at different scales to organize cell, tissue and embryo morphogenesis. We will specifically ask how the subcellular distribution of adhesion molecules controls the formation of cell-cell contacts, how cell-cell contacts determine tissue shape, and how tissue interactions regulate embryo morphogenesis.","lang":"eng"}],"issue":"1","publist_id":"3423","title":"Cell adhesion in embryo morphogenesis","publication_status":"published","status":"public","department":[{"_id":"CaHe"}],"publisher":"Elsevier","intvolume":" 24","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"3246","year":"2012","acknowledgement":"This review comes from a themed issue on Cell structure and dynamics Edited by Jason Swedlow and Gaudenz Danuser","date_created":"2018-12-11T12:02:14Z","date_updated":"2023-09-07T12:05:08Z","volume":24,"oa_version":"None","author":[{"full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone","first_name":"Vanessa"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"related_material":{"record":[{"id":"961","relation":"dissertation_contains","status":"public"}]}},{"month":"01","doi":"10.1073/pnas.1010767108","language":[{"iso":"eng"}],"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024655","open_access":"1"}],"oa":1,"external_id":{"pmid":["21212360"]},"quality_controlled":"1","publist_id":"3244","author":[{"full_name":"Krens, Gabriel","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996"},{"full_name":"Möllmert, Stephanie","last_name":"Möllmert","first_name":"Stephanie","id":"260FD49C-E911-11E9-B5EA-D9538404589B"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"volume":108,"date_updated":"2021-01-12T07:43:00Z","date_created":"2018-12-11T12:02:56Z","pmid":1,"year":"2011","publisher":"National Academy of Sciences","department":[{"_id":"CaHe"}],"publication_status":"published","day":"18","scopus_import":1,"date_published":"2011-01-18T00:00:00Z","citation":{"chicago":"Krens, Gabriel, Stephanie Möllmert, and Carl-Philipp J Heisenberg. “Enveloping Cell Layer Differentiation at the Surface of Zebrafish Germ Layer Tissue Explants.” PNAS. National Academy of Sciences, 2011. https://doi.org/10.1073/pnas.1010767108.","short":"G. Krens, S. Möllmert, C.-P.J. Heisenberg, PNAS 108 (2011) E9–E10.","mla":"Krens, Gabriel, et al. “Enveloping Cell Layer Differentiation at the Surface of Zebrafish Germ Layer Tissue Explants.” PNAS, vol. 108, no. 3, National Academy of Sciences, 2011, pp. E9–10, doi:10.1073/pnas.1010767108.","apa":"Krens, G., Möllmert, S., & Heisenberg, C.-P. J. (2011). Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1010767108","ieee":"G. Krens, S. Möllmert, and C.-P. J. Heisenberg, “Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants,” PNAS, vol. 108, no. 3. National Academy of Sciences, pp. E9–E10, 2011.","ista":"Krens G, Möllmert S, Heisenberg C-PJ. 2011. Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. PNAS. 108(3), E9–E10.","ama":"Krens G, Möllmert S, Heisenberg C-PJ. Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants. PNAS. 2011;108(3):E9-E10. doi:10.1073/pnas.1010767108"},"publication":"PNAS","page":"E9 - E10","issue":"3","abstract":[{"text":"Tissue surface tension (TST) is an important mechanical property influencing cell sorting and tissue envelopment. The study by Manning et al. (1) reported on a mathematical model describing TST on the basis of the balance between adhesive and tensile properties of the constituent cells. The model predicts that, in high-adhesion cell aggregates, surface cells will be stretched to maintain the same area of cell–cell contact as interior bulk cells, resulting in an elongated and flattened cell shape. The authors (1) observed flat and elongated cells at the surface of high-adhesion zebrafish germ-layer explants, which they argue are undifferentiated stretched germ-layer progenitor cells, and they use this observation as a validation of their model.","lang":"eng"}],"type":"journal_article","oa_version":"Submitted Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"3368","intvolume":" 108","status":"public","title":"Enveloping cell layer differentiation at the surface of zebrafish germ layer tissue explants"},{"intvolume":" 138","title":"Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube","ddc":["570"],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"3396","oa_version":"Published Version","file":[{"access_level":"open_access","file_name":"2011_Development_Stockinger.pdf","file_size":4672439,"content_type":"application/pdf","creator":"dernst","relation":"main_file","file_id":"6930","checksum":"ca12b79e01ef36c1ef1aea31cf7e7139","date_updated":"2020-07-14T12:46:12Z","date_created":"2019-10-07T14:19:42Z"}],"type":"journal_article","issue":"21","abstract":[{"text":"Facial branchiomotor neurons (FBMNs) in zebrafish and mouse embryonic hindbrain undergo a characteristic tangential migration from rhombomere (r) 4, where they are born, to r6/7. Cohesion among neuroepithelial cells (NCs) has been suggested to function in FBMN migration by inhibiting FBMNs positioned in the basal neuroepithelium such that they move apically between NCs towards the midline of the neuroepithelium instead of tangentially along the basal side of the neuroepithelium towards r6/7. However, direct experimental evaluation of this hypothesis is still lacking. Here, we have used a combination of biophysical cell adhesion measurements and high-resolution time-lapse microscopy to determine the role of NC cohesion in FBMN migration. We show that reducing NC cohesion by interfering with Cadherin 2 (Cdh2) activity results in FBMNs positioned at the basal side of the neuroepithelium moving apically towards the neural tube midline instead of tangentially towards r6/7. In embryos with strongly reduced NC cohesion, ectopic apical FBMN movement frequently results in fusion of the bilateral FBMN clusters over the apical midline of the neural tube. By contrast, reducing cohesion among FBMNs by interfering with Contactin 2 (Cntn2) expression in these cells has little effect on apical FBMN movement, but reduces the fusion of the bilateral FBMN clusters in embryos with strongly diminished NC cohesion. These data provide direct experimental evidence that NC cohesion functions in tangential FBMN migration by restricting their apical movement.","lang":"eng"}],"page":"4673 - 4683","article_type":"original","citation":{"chicago":"Stockinger, Petra, Carl-Philipp J Heisenberg, and Jean-Léon Maître. “Defective Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” Development. Company of Biologists, 2011. https://doi.org/10.1242/dev.071233.","mla":"Stockinger, Petra, et al. “Defective Neuroepithelial Cell Cohesion Affects Tangential Branchiomotor Neuron Migration in the Zebrafish Neural Tube.” Development, vol. 138, no. 21, Company of Biologists, 2011, pp. 4673–83, doi:10.1242/dev.071233.","short":"P. Stockinger, C.-P.J. Heisenberg, J.-L. Maître, Development 138 (2011) 4673–4683.","ista":"Stockinger P, Heisenberg C-PJ, Maître J-L. 2011. Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. Development. 138(21), 4673–4683.","ieee":"P. Stockinger, C.-P. J. Heisenberg, and J.-L. Maître, “Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube,” Development, vol. 138, no. 21. Company of Biologists, pp. 4673–4683, 2011.","apa":"Stockinger, P., Heisenberg, C.-P. J., & Maître, J.-L. (2011). Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. Development. Company of Biologists. https://doi.org/10.1242/dev.071233","ama":"Stockinger P, Heisenberg C-PJ, Maître J-L. Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube. Development. 2011;138(21):4673-4683. doi:10.1242/dev.071233"},"publication":"Development","date_published":"2011-09-28T00:00:00Z","scopus_import":1,"has_accepted_license":"1","day":"28","publisher":"Company of Biologists","department":[{"_id":"CaHe"}],"publication_status":"published","year":"2011","volume":138,"date_updated":"2021-01-12T07:43:11Z","date_created":"2018-12-11T12:03:06Z","author":[{"full_name":"Stockinger, Petra","last_name":"Stockinger","first_name":"Petra","id":"261CB030-E90D-11E9-B182-F697D44B663C"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"full_name":"Maître, Jean-Léon","last_name":"Maître","first_name":"Jean-Léon","orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87"}],"publist_id":"3210","file_date_updated":"2020-07-14T12:46:12Z","quality_controlled":"1","oa":1,"language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"doi":"10.1242/dev.071233","month":"09"},{"intvolume":" 23","publisher":"Elsevier","department":[{"_id":"CaHe"}],"status":"public","title":"The role of adhesion energy in controlling cell-cell contacts","publication_status":"published","_id":"3397","year":"2011","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","oa_version":"Submitted Version","volume":23,"date_created":"2018-12-11T12:03:06Z","date_updated":"2021-01-12T07:43:12Z","author":[{"id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474","first_name":"Jean-Léon","last_name":"Maître","full_name":"Maître, Jean-Léon"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"type":"journal_article","publist_id":"3211","issue":"5","abstract":[{"lang":"eng","text":"Recent advances in microscopy techniques and biophysical measurements have provided novel insight into the molecular, cellular and biophysical basis of cell adhesion. However, comparably little is known about a core element of cell–cell adhesion—the energy of adhesion at the cell–cell contact. In this review, we discuss approaches to understand the nature and regulation of adhesion energy, and propose strategies to determine adhesion energy between cells in vitro and in vivo."}],"page":"508 - 514","quality_controlled":"1","oa":1,"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188705/","open_access":"1"}],"citation":{"short":"J.-L. Maître, C.-P.J. Heisenberg, Current Opinion in Cell Biology 23 (2011) 508–514.","mla":"Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “The Role of Adhesion Energy in Controlling Cell-Cell Contacts.” Current Opinion in Cell Biology, vol. 23, no. 5, Elsevier, 2011, pp. 508–14, doi:10.1016/j.ceb.2011.07.004.","chicago":"Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “The Role of Adhesion Energy in Controlling Cell-Cell Contacts.” Current Opinion in Cell Biology. Elsevier, 2011. https://doi.org/10.1016/j.ceb.2011.07.004.","ama":"Maître J-L, Heisenberg C-PJ. The role of adhesion energy in controlling cell-cell contacts. Current Opinion in Cell Biology. 2011;23(5):508-514. doi:10.1016/j.ceb.2011.07.004","ieee":"J.-L. Maître and C.-P. J. Heisenberg, “The role of adhesion energy in controlling cell-cell contacts,” Current Opinion in Cell Biology, vol. 23, no. 5. Elsevier, pp. 508–514, 2011.","apa":"Maître, J.-L., & Heisenberg, C.-P. J. (2011). The role of adhesion energy in controlling cell-cell contacts. Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2011.07.004","ista":"Maître J-L, Heisenberg C-PJ. 2011. The role of adhesion energy in controlling cell-cell contacts. Current Opinion in Cell Biology. 23(5), 508–514."},"publication":"Current Opinion in Cell Biology","language":[{"iso":"eng"}],"doi":"10.1016/j.ceb.2011.07.004","date_published":"2011-10-01T00:00:00Z","scopus_import":1,"day":"01","month":"10"},{"department":[{"_id":"CaHe"}],"publisher":"Elsevier","publication_status":"published","pmid":1,"year":"2011","volume":354,"date_updated":"2021-01-12T07:43:04Z","date_created":"2018-12-11T12:03:00Z","author":[{"full_name":"Row, Richard","last_name":"Row","first_name":"Richard"},{"first_name":"Jean-Léon","last_name":"Maître","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474","full_name":"Maître, Jean-Léon"},{"first_name":"Benjamin","last_name":"Martin","full_name":"Martin, Benjamin"},{"id":"261CB030-E90D-11E9-B182-F697D44B663C","first_name":"Petra","last_name":"Stockinger","full_name":"Stockinger, Petra"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kimelman, David","first_name":"David","last_name":"Kimelman"}],"publist_id":"3228","quality_controlled":"1","oa":1,"external_id":{"pmid":["1463614"]},"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090540/"}],"language":[{"iso":"eng"}],"doi":"10.1016/j.ydbio.2011.03.025","month":"06","intvolume":" 354","title":"Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"3379","oa_version":"Submitted Version","type":"journal_article","issue":"1","abstract":[{"text":"The process of gastrulation is highly conserved across vertebrates on both the genetic and morphological levels, despite great variety in embryonic shape and speed of development. This mechanism spatially separates the germ layers and establishes the organizational foundation for future development. Mesodermal identity is specified in a superficial layer of cells, the epiblast, where cells maintain an epithelioid morphology. These cells involute to join the deeper hypoblast layer where they adopt a migratory, mesenchymal morphology. Expression of a cascade of related transcription factors orchestrates the parallel genetic transition from primitive to mature mesoderm. Although the early and late stages of this process are increasingly well understood, the transition between them has remained largely mysterious. We present here the first high resolution in vivo observations of the blebby transitional morphology of involuting mesodermal cells in a vertebrate embryo. We further demonstrate that the zebrafish spadetail mutation creates a reversible block in the maturation program, stalling cells in the transition state. This mutation creates an ideal system for dissecting the specific properties of cells undergoing the morphological transition of maturing mesoderm, as we demonstrate with a direct measurement of cell–cell adhesion.","lang":"eng"}],"page":"102 - 110","article_type":"original","citation":{"ieee":"R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P. J. Heisenberg, and D. Kimelman, “Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail,” Developmental Biology, vol. 354, no. 1. Elsevier, pp. 102–110, 2011.","apa":"Row, R., Maître, J.-L., Martin, B., Stockinger, P., Heisenberg, C.-P. J., & Kimelman, D. (2011). Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. Developmental Biology. Elsevier. https://doi.org/10.1016/j.ydbio.2011.03.025","ista":"Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. 2011. Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. Developmental Biology. 354(1), 102–110.","ama":"Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail. Developmental Biology. 2011;354(1):102-110. doi:10.1016/j.ydbio.2011.03.025","chicago":"Row, Richard, Jean-Léon Maître, Benjamin Martin, Petra Stockinger, Carl-Philipp J Heisenberg, and David Kimelman. “Completion of the Epithelial to Mesenchymal Transition in Zebrafish Mesoderm Requires Spadetail.” Developmental Biology. Elsevier, 2011. https://doi.org/10.1016/j.ydbio.2011.03.025.","short":"R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P.J. Heisenberg, D. Kimelman, Developmental Biology 354 (2011) 102–110.","mla":"Row, Richard, et al. “Completion of the Epithelial to Mesenchymal Transition in Zebrafish Mesoderm Requires Spadetail.” Developmental Biology, vol. 354, no. 1, Elsevier, 2011, pp. 102–10, doi:10.1016/j.ydbio.2011.03.025."},"publication":"Developmental Biology","date_published":"2011-06-01T00:00:00Z","scopus_import":1,"day":"01"},{"doi":"10.1111/j.1742-4658.2011.08136.x","date_published":"2011-07-01T00:00:00Z","language":[{"iso":"eng"}],"citation":{"mla":"Heisenberg, Carl-Philipp J. “Invited Lectures ‐ Symposia Area.” FEBS Journal, vol. 278, no. S1, Wiley-Blackwell, 2011, pp. 24–24, doi:10.1111/j.1742-4658.2011.08136.x.","short":"C.-P.J. Heisenberg, FEBS Journal 278 (2011) 24–24.","chicago":"Heisenberg, Carl-Philipp J. “Invited Lectures ‐ Symposia Area.” FEBS Journal. Wiley-Blackwell, 2011. https://doi.org/10.1111/j.1742-4658.2011.08136.x.","ama":"Heisenberg C-PJ. Invited Lectures ‐ Symposia Area. FEBS Journal. 2011;278(S1):24-24. doi:10.1111/j.1742-4658.2011.08136.x","ista":"Heisenberg C-PJ. 2011. Invited Lectures ‐ Symposia Area. FEBS Journal. 278(S1), 24–24.","apa":"Heisenberg, C.-P. J. (2011). Invited Lectures ‐ Symposia Area. FEBS Journal. Wiley-Blackwell. https://doi.org/10.1111/j.1742-4658.2011.08136.x","ieee":"C.-P. J. Heisenberg, “Invited Lectures ‐ Symposia Area,” FEBS Journal, vol. 278, no. S1. Wiley-Blackwell, pp. 24–24, 2011."},"publication":"FEBS Journal","page":"24 - 24","day":"01","month":"07","author":[{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"oa_version":"None","volume":278,"date_updated":"2021-01-12T07:43:06Z","date_created":"2018-12-11T12:03:01Z","_id":"3383","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","year":"2011","publisher":"Wiley-Blackwell","intvolume":" 278","department":[{"_id":"CaHe"}],"publication_status":"published","status":"public","title":"Invited Lectures ‐ Symposia Area","issue":"S1","publist_id":"3224","type":"journal_article"},{"article_processing_charge":"No","day":"01","month":"01","scopus_import":"1","language":[{"iso":"eng"}],"date_published":"2011-01-01T00:00:00Z","doi":"10.1016/B978-0-12-385065-2.00006-2","page":"189 - 213","quality_controlled":"1","citation":{"chicago":"Krens, Gabriel, and Carl-Philipp J Heisenberg. “Cell Sorting in Development.” In Forces and Tension in Development, edited by Michel Labouesse, 95:189–213. Elsevier, 2011. https://doi.org/10.1016/B978-0-12-385065-2.00006-2.","mla":"Krens, Gabriel, and Carl-Philipp J. Heisenberg. “Cell Sorting in Development.” Forces and Tension in Development, edited by Michel Labouesse, vol. 95, Elsevier, 2011, pp. 189–213, doi:10.1016/B978-0-12-385065-2.00006-2.","short":"G. Krens, C.-P.J. Heisenberg, in:, M. Labouesse (Ed.), Forces and Tension in Development, Elsevier, 2011, pp. 189–213.","ista":"Krens G, Heisenberg C-PJ. 2011.Cell sorting in development. In: Forces and Tension in Development. Current Topics in Developmental Biology, vol. 95, 189–213.","apa":"Krens, G., & Heisenberg, C.-P. J. (2011). Cell sorting in development. In M. Labouesse (Ed.), Forces and Tension in Development (Vol. 95, pp. 189–213). Elsevier. https://doi.org/10.1016/B978-0-12-385065-2.00006-2","ieee":"G. Krens and C.-P. J. Heisenberg, “Cell sorting in development,” in Forces and Tension in Development, vol. 95, M. Labouesse, Ed. Elsevier, 2011, pp. 189–213.","ama":"Krens G, Heisenberg C-PJ. Cell sorting in development. In: Labouesse M, ed. Forces and Tension in Development. Vol 95. Elsevier; 2011:189-213. doi:10.1016/B978-0-12-385065-2.00006-2"},"publication":"Forces and Tension in Development","publist_id":"2436","abstract":[{"lang":"eng","text":"During the development of multicellular organisms, cell fate specification is followed by the sorting of different cell types into distinct domains from where the different tissues and organs are formed. Cell sorting involves both the segregation of a mixed population of cells with different fates and properties into distinct domains, and the active maintenance of their segregated state. Because of its biological importance and apparent resemblance to fluid segregation in physics, cell sorting was extensively studied by both biologists and physicists over the last decades. Different theories were developed that try to explain cell sorting on the basis of the physical properties of the constituent cells. However, only recently the molecular and cellular mechanisms that control the physical properties driving cell sorting, have begun to be unraveled. In this review, we will provide an overview of different cell-sorting processes in development and discuss how these processes can be explained by the different sorting theories, and how these theories in turn can be connected to the molecular and cellular mechanisms driving these processes."}],"alternative_title":["Current Topics in Developmental Biology"],"type":"book_chapter","volume":95,"oa_version":"None","date_updated":"2021-01-12T07:52:13Z","date_created":"2018-12-11T12:05:11Z","author":[{"last_name":"Krens","first_name":"Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"department":[{"_id":"CaHe"}],"editor":[{"first_name":"Michel","last_name":"Labouesse","full_name":"Labouesse, Michel"}],"intvolume":" 95","publisher":"Elsevier","title":"Cell sorting in development","status":"public","publication_status":"published","_id":"3791","year":"2011","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"author":[{"full_name":"Oteíza, Pablo","last_name":"Oteíza","first_name":"Pablo"},{"last_name":"Koeppen","first_name":"Mathias","full_name":"Koeppen, Mathias"},{"last_name":"Krieg","first_name":"Michael","full_name":"Krieg, Michael"},{"full_name":"Pulgar, Eduardo","last_name":"Pulgar","first_name":"Eduardo"},{"full_name":"Farias, Cecilia","last_name":"Farias","first_name":"Cecilia"},{"last_name":"Melo","first_name":"Cristina","full_name":"Melo, Cristina"},{"full_name":"Preibisch, Steffen","first_name":"Steffen","last_name":"Preibisch"},{"first_name":"Daniel","last_name":"Mueller","full_name":"Mueller, Daniel"},{"full_name":"Tada, Masazumi","first_name":"Masazumi","last_name":"Tada"},{"full_name":"Hartel, Steffen","first_name":"Steffen","last_name":"Hartel"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Miguel","last_name":"Concha","full_name":"Concha, Miguel"}],"volume":137,"oa_version":"None","date_created":"2018-12-11T12:07:20Z","date_updated":"2021-01-12T07:54:58Z","year":"2010","_id":"4163","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Company of Biologists","intvolume":" 137","status":"public","publication_status":"published","title":"Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ","publist_id":"1958","issue":"20","abstract":[{"text":"Organ formation requires the precise assembly of progenitor cells into a functional multicellular structure. Mechanical forces probably participate in this process but how they influence organ morphogenesis is still unclear. Here, we show that Wnt11- and Prickle1a-mediated planar cell polarity (PCP) signalling coordinates the formation of the zebrafish ciliated laterality organ (Kupffer's vesicle) by regulating adhesion properties between organ progenitor cells (the dorsal forerunner cells, DFCs). Combined inhibition of Wnt11 and Prickle1a reduces DFC cell-cell adhesion and impairs their compaction and arrangement during vesicle lumen formation. This leads to the formation of a mis-shapen vesicle with small fragmented lumina and shortened cilia, resulting in severely impaired organ function and, as a consequence, randomised laterality of both molecular and visceral asymmetries. Our results reveal a novel role for PCP-dependent cell adhesion in coordinating the supracellular organisation of progenitor cells during vertebrate laterality organ formation.","lang":"eng"}],"extern":"1","type":"journal_article","date_published":"2010-10-15T00:00:00Z","doi":"10.1242/dev.049981","language":[{"iso":"eng"}],"citation":{"ama":"Oteíza P, Koeppen M, Krieg M, et al. Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ. Development. 2010;137(20):3459-3468. doi:10.1242/dev.049981","ista":"Oteíza P, Koeppen M, Krieg M, Pulgar E, Farias C, Melo C, Preibisch S, Mueller D, Tada M, Hartel S, Heisenberg C-PJ, Concha M. 2010. Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ. Development. 137(20), 3459–3468.","apa":"Oteíza, P., Koeppen, M., Krieg, M., Pulgar, E., Farias, C., Melo, C., … Concha, M. (2010). Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ. Development. Company of Biologists. https://doi.org/10.1242/dev.049981","ieee":"P. Oteíza et al., “Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ,” Development, vol. 137, no. 20. Company of Biologists, pp. 3459–3468, 2010.","mla":"Oteíza, Pablo, et al. “Planar Cell Polarity Signalling Regulates Cell Adhesion Properties in Progenitors of the Zebrafish Laterality Organ.” Development, vol. 137, no. 20, Company of Biologists, 2010, pp. 3459–68, doi:10.1242/dev.049981.","short":"P. Oteíza, M. Koeppen, M. Krieg, E. Pulgar, C. Farias, C. Melo, S. Preibisch, D. Mueller, M. Tada, S. Hartel, C.-P.J. Heisenberg, M. Concha, Development 137 (2010) 3459–3468.","chicago":"Oteíza, Pablo, Mathias Koeppen, Michael Krieg, Eduardo Pulgar, Cecilia Farias, Cristina Melo, Steffen Preibisch, et al. “Planar Cell Polarity Signalling Regulates Cell Adhesion Properties in Progenitors of the Zebrafish Laterality Organ.” Development. Company of Biologists, 2010. https://doi.org/10.1242/dev.049981."},"publication":"Development","page":"3459 - 3468","article_processing_charge":"No","month":"10","day":"15"},{"month":"01","day":"01","article_processing_charge":"No","page":"273 - 287","publication":"Methods in Molecular Biology","citation":{"short":"L. Carvalho, C.-P.J. Heisenberg, Methods in Molecular Biology 546 (2010) 273–287.","mla":"Carvalho, Lara, and Carl-Philipp J. Heisenberg. “Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy.” Methods in Molecular Biology, vol. 546, no. Part 5, Springer, 2010, pp. 273–87, doi:10.1007/978-1-60327-977-2_17.","chicago":"Carvalho, Lara, and Carl-Philipp J Heisenberg. “Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy.” Methods in Molecular Biology. Springer, 2010. https://doi.org/10.1007/978-1-60327-977-2_17.","ama":"Carvalho L, Heisenberg C-PJ. Imaging zebrafish embryos by two-photon excitation time-lapse microscopy. Methods in Molecular Biology. 2010;546(Part 5):273-287. doi:10.1007/978-1-60327-977-2_17","ieee":"L. Carvalho and C.-P. J. Heisenberg, “Imaging zebrafish embryos by two-photon excitation time-lapse microscopy,” Methods in Molecular Biology, vol. 546, no. Part 5. Springer, pp. 273–287, 2010.","apa":"Carvalho, L., & Heisenberg, C.-P. J. (2010). Imaging zebrafish embryos by two-photon excitation time-lapse microscopy. Methods in Molecular Biology. Springer. https://doi.org/10.1007/978-1-60327-977-2_17","ista":"Carvalho L, Heisenberg C-PJ. 2010. Imaging zebrafish embryos by two-photon excitation time-lapse microscopy. Methods in Molecular Biology. 546(Part 5), 273–287."},"language":[{"iso":"eng"}],"doi":"10.1007/978-1-60327-977-2_17","date_published":"2010-01-01T00:00:00Z","type":"journal_article","extern":"1","abstract":[{"lang":"eng","text":"The zebrafish is a favorite model organism to study tissue morphogenesis during development at a subcellular level. This largely results from the fact that zebrafish embryos are transparent and thus accessible to various imaging techniques, such as confocal and two-photon excitation (2PE) microscopy. In particular, 2PE microscopy has been shown to be useful for imaging deep cell layers within the embryo and following tissue morphogenesis over long periods. This chapter describes how to use 2PE microscopy to study morphogenetic movements during early zebrafish embryonic development, providing a general blueprint for its use in zebrafish."}],"publist_id":"2791","issue":"Part 5","status":"public","publication_status":"published","title":"Imaging zebrafish embryos by two-photon excitation time-lapse microscopy","intvolume":" 546","publisher":"Springer","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"3592","year":"2010","date_created":"2018-12-11T12:04:08Z","date_updated":"2021-01-12T07:44:31Z","oa_version":"None","volume":546,"author":[{"full_name":"Carvalho, Lara","last_name":"Carvalho","first_name":"Lara"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}]},{"doi":"10.1371/journal.pbio.1000544","language":[{"iso":"eng"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"quality_controlled":"1","month":"11","author":[{"first_name":"Alba","last_name":"Diz Muñoz","full_name":"Diz Muñoz, Alba"},{"first_name":"Michael","last_name":"Krieg","full_name":"Krieg, Michael"},{"full_name":"Bergert, Martin","first_name":"Martin","last_name":"Bergert"},{"last_name":"Ibarlucea Benitez","first_name":"Itziar","full_name":"Ibarlucea Benitez, Itziar"},{"full_name":"Müller, Daniel","first_name":"Daniel","last_name":"Müller"},{"last_name":"Paluch","first_name":"Ewa","full_name":"Paluch, Ewa"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"volume":8,"date_created":"2018-12-11T12:05:11Z","date_updated":"2021-01-12T07:52:13Z","acknowledgement":"We would like to thank A. G. Clark, S. Grill, A. Oates, E. Raz, L. Rohde, and M. Zerial for reading earlier versions of the manuscript. We are grateful to W. Zachariae, Y. Arboleda-Estudillo, S. Schneider, P. Stockinger, D. Panhans, M. Biro, J. C. Olaya, and the BIOTEC/MPI-CBG zebrafish and imaging facilities for help and advice at various stages of this project and to J. Helenius for help with programming. This work was supported by grants from the Boehringer Ingelheim Fonds to MK, the Polish Ministry of Science and Higher Education to E. P., and the Deutsche Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EP.\r\n","year":"2010","department":[{"_id":"CaHe"}],"publisher":"Public Library of Science","publication_status":"published","publist_id":"2437","file_date_updated":"2020-07-14T12:46:16Z","article_number":"e1000544","date_published":"2010-11-30T00:00:00Z","citation":{"mla":"Diz Muñoz, Alba, et al. “Control of Directed Cell Migration in Vivo by Membrane-to-Cortex Attachment.” PLoS Biology, vol. 8, no. 11, e1000544, Public Library of Science, 2010, doi:10.1371/journal.pbio.1000544.","short":"A. Diz Muñoz, M. Krieg, M. Bergert, I. Ibarlucea Benitez, D. Müller, E. Paluch, C.-P.J. Heisenberg, PLoS Biology 8 (2010).","chicago":"Diz Muñoz, Alba, Michael Krieg, Martin Bergert, Itziar Ibarlucea Benitez, Daniel Müller, Ewa Paluch, and Carl-Philipp J Heisenberg. “Control of Directed Cell Migration in Vivo by Membrane-to-Cortex Attachment.” PLoS Biology. Public Library of Science, 2010. https://doi.org/10.1371/journal.pbio.1000544.","ama":"Diz Muñoz A, Krieg M, Bergert M, et al. Control of directed cell migration in vivo by membrane-to-cortex attachment. PLoS Biology. 2010;8(11). doi:10.1371/journal.pbio.1000544","ista":"Diz Muñoz A, Krieg M, Bergert M, Ibarlucea Benitez I, Müller D, Paluch E, Heisenberg C-PJ. 2010. Control of directed cell migration in vivo by membrane-to-cortex attachment. PLoS Biology. 8(11), e1000544.","apa":"Diz Muñoz, A., Krieg, M., Bergert, M., Ibarlucea Benitez, I., Müller, D., Paluch, E., & Heisenberg, C.-P. J. (2010). Control of directed cell migration in vivo by membrane-to-cortex attachment. PLoS Biology. Public Library of Science. https://doi.org/10.1371/journal.pbio.1000544","ieee":"A. Diz Muñoz et al., “Control of directed cell migration in vivo by membrane-to-cortex attachment,” PLoS Biology, vol. 8, no. 11. Public Library of Science, 2010."},"publication":"PLoS Biology","has_accepted_license":"1","day":"30","scopus_import":1,"pubrep_id":"365","file":[{"date_updated":"2020-07-14T12:46:16Z","date_created":"2018-12-12T10:08:24Z","checksum":"52d18c90ca6b02234cea5e8b399b7f46","file_id":"4685","relation":"main_file","creator":"system","file_size":799506,"content_type":"application/pdf","file_name":"IST-2015-365-v1+1_journal.pbio.1000544.pdf","access_level":"open_access"}],"oa_version":"Published Version","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"3790","intvolume":" 8","ddc":["576"],"status":"public","title":"Control of directed cell migration in vivo by membrane-to-cortex attachment","issue":"11","abstract":[{"lang":"eng","text":"Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to-cortex attachment (MCA) in cell protrusion formation and migration, in particular in the context of the developing embryo. Here, we use a multidisciplinary approach to study MCA in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells, which migrate using a combination of different protrusion types, namely, lamellipodia, filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity of molecules linking the cortex to the membrane and measuring resulting changes in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors increases the proportion of cellular blebs and reduces the directionality of cell migration. We propose that MCA is a key parameter controlling the relative proportions of different cell protrusion types in mesendoderm progenitors, and thus is key in controlling directed migration during gastrulation."}],"type":"journal_article"},{"issue":"21","publist_id":"2438","abstract":[{"text":"The development of multicellular organisms is dependent on the tight coordination between tissue growth and morphogenesis. The stereotypical orientation of cell divisions has been proposed to be a fundamental mechanism by which proliferating and growing tissues take shape. However, the actual contribution of stereotypical division orientation (SDO) to tissue morphogenesis is unclear. In zebrafish, cell divisions with stereotypical orientation have been implicated in both body-axis elongation and neural rod formation [1, 2], although there is little direct evidence for a critical function of SDO in either of these processes. Here we show that SDO is required for formation of the neural rod midline during neurulation but dispensable for elongation of the body axis during gastrulation. Our data indicate that SDO during both gastrulation and neurulation is dependent on the noncanonical Wnt receptor Frizzled 7 (Fz7) and that interfering with cell division orientation leads to severe defects in neural rod midline formation but not body-axis elongation. These findings suggest a novel function for Fz7-controlled cell division orientation in neural rod midline formation during neurulation. ","lang":"eng"}],"type":"journal_article","author":[{"id":"EA35229E-E909-11E9-8DF8-C90C5D5AF86E","last_name":"Quesada-Hernández","first_name":"Elena","full_name":"Quesada-Hernández, Elena"},{"full_name":"Caneparo, Luca","last_name":"Caneparo","first_name":"Luca"},{"full_name":"Schneider, Sylvia","id":"1FAC36B0-E90A-11E9-9D2F-EF31CE0C9C2F","last_name":"Schneider","first_name":"Sylvia"},{"full_name":"Winkler, Sylke","first_name":"Sylke","last_name":"Winkler"},{"first_name":"Michael","last_name":"Liebling","full_name":"Liebling, Michael"},{"full_name":"Fraser, Scott","first_name":"Scott","last_name":"Fraser"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"oa_version":"None","volume":20,"date_created":"2018-12-11T12:05:11Z","date_updated":"2021-01-12T07:52:12Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"3789","acknowledgement":"This work was supported by grants from the Fundacion Caja Madrid to E.Q.H. and the Institute of Science and Technology Austria, the Max-Planck-Society, and the Deutsche Forschungsgemeinschaft to C.P.H.\r\nWe are grateful to Jon Clarke, Andy Oates, and Garrett Greenan for reading earlier versions of this manuscript. We thank J. Peychl, H. Ibarra, and P. Pitrone for excellent assistance and advice in multi-photon microscopy and D. White for assistance during the image-processing steps. We also thank D. Panhans for technical assistance, the whole Heisenberg laboratory for useful comments and discussions, and E. Lehmann, J. Hückmann, and G. Junghans for excellent fish care. ","year":"2010","publisher":"Cell Press","department":[{"_id":"CaHe"}],"intvolume":" 20","title":"Stereotypical cell division orientation controls neural rod midline formation in zebrafish","status":"public","publication_status":"published","day":"09","month":"11","scopus_import":1,"date_published":"2010-11-09T00:00:00Z","doi":"10.1016/j.cub.2010.10.009","language":[{"iso":"eng"}],"citation":{"ama":"Quesada-Hernández E, Caneparo L, Schneider S, et al. Stereotypical cell division orientation controls neural rod midline formation in zebrafish. Current Biology. 2010;20(21):1966-1972. doi:10.1016/j.cub.2010.10.009","ista":"Quesada-Hernández E, Caneparo L, Schneider S, Winkler S, Liebling M, Fraser S, Heisenberg C-PJ. 2010. Stereotypical cell division orientation controls neural rod midline formation in zebrafish. Current Biology. 20(21), 1966–1972.","ieee":"E. Quesada-Hernández et al., “Stereotypical cell division orientation controls neural rod midline formation in zebrafish,” Current Biology, vol. 20, no. 21. Cell Press, pp. 1966–1972, 2010.","apa":"Quesada-Hernández, E., Caneparo, L., Schneider, S., Winkler, S., Liebling, M., Fraser, S., & Heisenberg, C.-P. J. (2010). Stereotypical cell division orientation controls neural rod midline formation in zebrafish. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2010.10.009","mla":"Quesada-Hernández, Elena, et al. “Stereotypical Cell Division Orientation Controls Neural Rod Midline Formation in Zebrafish.” Current Biology, vol. 20, no. 21, Cell Press, 2010, pp. 1966–72, doi:10.1016/j.cub.2010.10.009.","short":"E. Quesada-Hernández, L. Caneparo, S. Schneider, S. Winkler, M. Liebling, S. Fraser, C.-P.J. Heisenberg, Current Biology 20 (2010) 1966–1972.","chicago":"Quesada-Hernández, Elena, Luca Caneparo, Sylvia Schneider, Sylke Winkler, Michael Liebling, Scott Fraser, and Carl-Philipp J Heisenberg. “Stereotypical Cell Division Orientation Controls Neural Rod Midline Formation in Zebrafish.” Current Biology. Cell Press, 2010. https://doi.org/10.1016/j.cub.2010.10.009."},"publication":"Current Biology","page":"1966 - 1972","quality_controlled":"1"},{"date_created":"2018-12-11T12:05:10Z","date_updated":"2021-01-12T07:52:12Z","volume":33,"oa_version":"None","author":[{"full_name":"Klopper, Abigail","first_name":"Abigail","last_name":"Klopper"},{"full_name":"Krens, Gabriel","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996"},{"full_name":"Grill, Stephan","first_name":"Stephan","last_name":"Grill"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J"}],"publication_status":"published","status":"public","title":"Finite-size corrections to scaling behavior in sorted cell aggregates","intvolume":" 33","publisher":"Springer","department":[{"_id":"CaHe"}],"_id":"3788","year":"2010","user_id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Cell sorting is a widespread phenomenon pivotal to the early development of multicellular organisms. In vitro cell sorting studies have been instrumental in revealing the cellular properties driving this process. However, these studies have as yet been limited to two-dimensional analysis of three-dimensional cell sorting events. Here we describe a method to record the sorting of primary zebrafish ectoderm and mesoderm germ layer progenitor cells in three dimensions over time, and quantitatively analyze their sorting behavior using an order parameter related to heterotypic interface length. We investigate the cell population size dependence of sorted aggregates and find that the germ layer progenitor cells engulfed in the final configuration display a relationship between total interfacial length and system size according to a simple geometrical argument, subject to a finite-size effect."}],"issue":"2","publist_id":"2439","type":"journal_article","language":[{"iso":"eng"}],"doi":"10.1140/epje/i2010-10642-y","date_published":"2010-09-18T00:00:00Z","page":"99 - 103","publication":"The European Physical Journal E: Soft Matter and Biological Physics","citation":{"chicago":"Klopper, Abigail, Gabriel Krens, Stephan Grill, and Carl-Philipp J Heisenberg. “Finite-Size Corrections to Scaling Behavior in Sorted Cell Aggregates.” The European Physical Journal E: Soft Matter and Biological Physics. Springer, 2010. https://doi.org/10.1140/epje/i2010-10642-y.","short":"A. Klopper, G. Krens, S. Grill, C.-P.J. Heisenberg, The European Physical Journal E: Soft Matter and Biological Physics 33 (2010) 99–103.","mla":"Klopper, Abigail, et al. “Finite-Size Corrections to Scaling Behavior in Sorted Cell Aggregates.” The European Physical Journal E: Soft Matter and Biological Physics, vol. 33, no. 2, Springer, 2010, pp. 99–103, doi:10.1140/epje/i2010-10642-y.","ieee":"A. Klopper, G. Krens, S. Grill, and C.-P. J. Heisenberg, “Finite-size corrections to scaling behavior in sorted cell aggregates,” The European Physical Journal E: Soft Matter and Biological Physics, vol. 33, no. 2. Springer, pp. 99–103, 2010.","apa":"Klopper, A., Krens, G., Grill, S., & Heisenberg, C.-P. J. (2010). Finite-size corrections to scaling behavior in sorted cell aggregates. The European Physical Journal E: Soft Matter and Biological Physics. Springer. https://doi.org/10.1140/epje/i2010-10642-y","ista":"Klopper A, Krens G, Grill S, Heisenberg C-PJ. 2010. Finite-size corrections to scaling behavior in sorted cell aggregates. The European Physical Journal E: Soft Matter and Biological Physics. 33(2), 99–103.","ama":"Klopper A, Krens G, Grill S, Heisenberg C-PJ. Finite-size corrections to scaling behavior in sorted cell aggregates. The European Physical Journal E: Soft Matter and Biological Physics. 2010;33(2):99-103. doi:10.1140/epje/i2010-10642-y"},"day":"18","month":"09","scopus_import":1},{"language":[{"iso":"eng"}],"date_published":"2010-10-01T00:00:00Z","doi":"10.1016/j.tcb.2010.06.009","page":"586 - 592","quality_controlled":"1","citation":{"chicago":"Carvalho, Lara, and Carl-Philipp J Heisenberg. “The Yolk Syncytial Layer in Early, Zebrafish Development.” Trends in Cell Biology. Cell Press, 2010. https://doi.org/10.1016/j.tcb.2010.06.009.","mla":"Carvalho, Lara, and Carl-Philipp J. Heisenberg. “The Yolk Syncytial Layer in Early, Zebrafish Development.” Trends in Cell Biology, vol. 20, no. 10, Cell Press, 2010, pp. 586–92, doi:10.1016/j.tcb.2010.06.009.","short":"L. Carvalho, C.-P.J. Heisenberg, Trends in Cell Biology 20 (2010) 586–592.","ista":"Carvalho L, Heisenberg C-PJ. 2010. The yolk syncytial layer in early, zebrafish development. Trends in Cell Biology. 20(10), 586–592.","apa":"Carvalho, L., & Heisenberg, C.-P. J. (2010). The yolk syncytial layer in early, zebrafish development. Trends in Cell Biology. Cell Press. https://doi.org/10.1016/j.tcb.2010.06.009","ieee":"L. Carvalho and C.-P. J. Heisenberg, “The yolk syncytial layer in early, zebrafish development,” Trends in Cell Biology, vol. 20, no. 10. Cell Press, pp. 586–592, 2010.","ama":"Carvalho L, Heisenberg C-PJ. The yolk syncytial layer in early, zebrafish development. Trends in Cell Biology. 2010;20(10):586-592. doi:10.1016/j.tcb.2010.06.009"},"publication":"Trends in Cell Biology","article_processing_charge":"No","day":"01","month":"10","scopus_import":"1","oa_version":"None","volume":20,"date_created":"2018-12-11T12:05:12Z","date_updated":"2022-08-25T15:00:19Z","author":[{"first_name":"Lara","last_name":"Carvalho","full_name":"Carvalho, Lara"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"intvolume":" 20","publisher":"Cell Press","publication_status":"published","title":"The yolk syncytial layer in early, zebrafish development","status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"3792","year":"2010","acknowledgement":"We are grateful to Valerie Virta and Jennifer Regan for reading earlier versions of the review.\r\n","publist_id":"2435","issue":"10","abstract":[{"lang":"eng","text":"The yolk syncytial layer (YSL) plays crucial roles in early zebrafish development. The YSL is a transient extra-embryonic syncytial tissue that forms during early cleavage stages and persists until larval stages. During gastrulation, the YSL undergoes highly dynamic movements, which are tightly coordinated with the movements of the overlying germ layer progenitor cells, and has critical functions in cell fate specification and morphogenesis of the early germ layers. Movement coordination between the YSL and blastoderm cells is dependent on contact between these tissues, and is probably required for the patterning and morphogenetic function of the YSL. In this review, we will discuss recent advances in elucidating the molecular and cellular mechanisms underlying the YSL morphogenesis and movement coordination between the YSL and blastoderm during early development."}],"type":"journal_article"},{"day":"18","scopus_import":1,"date_published":"2010-08-18T00:00:00Z","page":"2753 - 2768","publication":"EMBO Journal","citation":{"mla":"Papusheva, Ekaterina, and Carl-Philipp J. Heisenberg. “Spatial Organization of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.” EMBO Journal, vol. 29, no. 16, Wiley-Blackwell, 2010, pp. 2753–68, doi:10.1038/emboj.2010.182.","short":"E. Papusheva, C.-P.J. Heisenberg, EMBO Journal 29 (2010) 2753–2768.","chicago":"Papusheva, Ekaterina, and Carl-Philipp J Heisenberg. “Spatial Organization of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.” EMBO Journal. Wiley-Blackwell, 2010. https://doi.org/10.1038/emboj.2010.182.","ama":"Papusheva E, Heisenberg C-PJ. Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. EMBO Journal. 2010;29(16):2753-2768. doi:10.1038/emboj.2010.182","ista":"Papusheva E, Heisenberg C-PJ. 2010. Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. EMBO Journal. 29(16), 2753–2768.","apa":"Papusheva, E., & Heisenberg, C.-P. J. (2010). Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis. EMBO Journal. Wiley-Blackwell. https://doi.org/10.1038/emboj.2010.182","ieee":"E. Papusheva and C.-P. J. Heisenberg, “Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis,” EMBO Journal, vol. 29, no. 16. Wiley-Blackwell, pp. 2753–2768, 2010."},"abstract":[{"text":"Integrin- and cadherin-mediated adhesion is central for cell and tissue morphogenesis, allowing cells and tissues to change shape without loosing integrity. Studies predominantly in cell culture showed that mechanosensation through adhesion structures is achieved by force-mediated modulation of their molecular composition. The specific molecular composition of adhesion sites in turn determines their signalling activity and dynamic reorganization. Here, we will review how adhesion sites respond to mecanical stimuli, and how spatially and temporally regulated signalling from different adhesion sites controls cell migration and tissue morphogenesis.","lang":"eng"}],"issue":"16","type":"journal_article","oa_version":"Submitted Version","title":"Spatial organization of adhesion: force-dependent regulation and function in tissue morphogenesis","status":"public","intvolume":" 29","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"4157","month":"08","acknowledged_ssus":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"doi":"10.1038/emboj.2010.182","quality_controlled":"1","external_id":{"pmid":["20717145"]},"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924654/"}],"oa":1,"publist_id":"1962","date_updated":"2021-01-12T07:54:55Z","date_created":"2018-12-11T12:07:17Z","volume":29,"author":[{"id":"41DB591E-F248-11E8-B48F-1D18A9856A87","last_name":"Papusheva","first_name":"Ekaterina","full_name":"Papusheva, Ekaterina"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"publication_status":"published","publisher":"Wiley-Blackwell","department":[{"_id":"Bio"},{"_id":"CaHe"}],"year":"2010","pmid":1},{"type":"journal_article","extern":"1","abstract":[{"text":"Collective cell migration, the simultaneous movement of multiple cells that are connected by cell-cell adhesion, is ubiquitous in development, tissue repair, and tumor metastasis [1, 2]. It has been hypothesized that the directionality of cell movement during collective migration emerges as a collective property [3, 4]. Here we determine how movement directionality is established in collective mesendoderm migration during zebrafish gastrulation. By interfering with two key features of collective migration, (1) having neighboring cells and (2) adhering to them, we show that individual mesendoderm cells are capable of normal directed migration when moving as single cells but require cell-cell adhesion to participate in coordinated and directed migration when moving as part of a group. We conclude that movement directionality is not a de novo collective property of mesendoderm cells but rather a property of single mesendoderm cells that requires cell-cell adhesion during collective migration.","lang":"eng"}],"publist_id":"1897","issue":"2","publication_status":"published","title":"Movement directionality in collective migration of germ layer progenitors","status":"public","intvolume":" 20","publisher":"Cell Press","year":"2010","_id":"4221","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T07:55:25Z","date_created":"2018-12-11T12:07:40Z","volume":20,"oa_version":"None","author":[{"full_name":"Arboleda Estudillo, Yohanna","last_name":"Arboleda Estudillo","first_name":"Yohanna"},{"full_name":"Krieg, Michael","last_name":"Krieg","first_name":"Michael"},{"full_name":"Stuehmer, Jan","last_name":"Stuehmer","first_name":"Jan"},{"full_name":"Licata, Nicholas","first_name":"Nicholas","last_name":"Licata"},{"first_name":"Daniel","last_name":"Mueller","full_name":"Mueller, Daniel"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"day":"26","month":"01","article_processing_charge":"No","page":"161 - 169","publication":"Current Biology","citation":{"ama":"Arboleda Estudillo Y, Krieg M, Stuehmer J, Licata N, Mueller D, Heisenberg C-PJ. Movement directionality in collective migration of germ layer progenitors. Current Biology. 2010;20(2):161-169. doi:10.1016/j.cub.2009.11.036","ieee":"Y. Arboleda Estudillo, M. Krieg, J. Stuehmer, N. Licata, D. Mueller, and C.-P. J. Heisenberg, “Movement directionality in collective migration of germ layer progenitors,” Current Biology, vol. 20, no. 2. Cell Press, pp. 161–169, 2010.","apa":"Arboleda Estudillo, Y., Krieg, M., Stuehmer, J., Licata, N., Mueller, D., & Heisenberg, C.-P. J. (2010). Movement directionality in collective migration of germ layer progenitors. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2009.11.036","ista":"Arboleda Estudillo Y, Krieg M, Stuehmer J, Licata N, Mueller D, Heisenberg C-PJ. 2010. Movement directionality in collective migration of germ layer progenitors. Current Biology. 20(2), 161–169.","short":"Y. Arboleda Estudillo, M. Krieg, J. Stuehmer, N. Licata, D. Mueller, C.-P.J. Heisenberg, Current Biology 20 (2010) 161–169.","mla":"Arboleda Estudillo, Yohanna, et al. “Movement Directionality in Collective Migration of Germ Layer Progenitors.” Current Biology, vol. 20, no. 2, Cell Press, 2010, pp. 161–69, doi:10.1016/j.cub.2009.11.036.","chicago":"Arboleda Estudillo, Yohanna, Michael Krieg, Jan Stuehmer, Nicholas Licata, Daniel Mueller, and Carl-Philipp J Heisenberg. “Movement Directionality in Collective Migration of Germ Layer Progenitors.” Current Biology. Cell Press, 2010. https://doi.org/10.1016/j.cub.2009.11.036."},"language":[{"iso":"eng"}],"date_published":"2010-01-26T00:00:00Z","doi":"10.1016/j.cub.2009.11.036"},{"article_processing_charge":"No","day":"01","month":"08","language":[{"iso":"eng"}],"date_published":"2009-08-01T00:00:00Z","doi":"10.1016/j.mod.2009.06.391","page":"S168 - S168","citation":{"short":"G. Soete, C.-P.J. Heisenberg, Mechanisms of Development 126 (2009) S168–S168.","mla":"Soete, Gwen, and Carl-Philipp J. Heisenberg. “The Role of the Extracellular Matrix in Kupffer’s Vesicle Formation in Zebrafish.” Mechanisms of Development, vol. 126, Elsevier, 2009, pp. S168–S168, doi:10.1016/j.mod.2009.06.391.","chicago":"Soete, Gwen, and Carl-Philipp J Heisenberg. “The Role of the Extracellular Matrix in Kupffer’s Vesicle Formation in Zebrafish.” Mechanisms of Development. Elsevier, 2009. https://doi.org/10.1016/j.mod.2009.06.391.","ama":"Soete G, Heisenberg C-PJ. The role of the extracellular matrix in Kupffer’s vesicle formation in zebrafish. Mechanisms of Development. 2009;126:S168-S168. doi:10.1016/j.mod.2009.06.391","apa":"Soete, G., & Heisenberg, C.-P. J. (2009). The role of the extracellular matrix in Kupffer’s vesicle formation in zebrafish. Mechanisms of Development. Elsevier. https://doi.org/10.1016/j.mod.2009.06.391","ieee":"G. Soete and C.-P. J. Heisenberg, “The role of the extracellular matrix in Kupffer’s vesicle formation in zebrafish,” Mechanisms of Development, vol. 126. Elsevier, pp. S168–S168, 2009.","ista":"Soete G, Heisenberg C-PJ. 2009. The role of the extracellular matrix in Kupffer’s vesicle formation in zebrafish. Mechanisms of Development. 126, S168–S168."},"publication":"Mechanisms of Development","extern":"1","publist_id":"1970","abstract":[{"text":"An important step in the formation of all epithelial organs is the coordinated polarisation of their constituent cells. One of the factors thought to be crucial for this process is the extracellular matrix (ECM), which provides positional information for cells and directs polarity specification and epithelial cyst formation in 3D culture. However, in vivo evidence for the role of the ECM in epithelial tissue polarisation is scarce.\r\n\r\nTo gain insight in the factors involved in establishing cell polarity during organogenesis, we are studying a group of epithelial cells called the Dorsal Forerunner Cells (DFCs) in zebrafish embryos. These cells migrate as a cluster towards the vegetal pole of the developing embryo, where they involute. During this process they polarise, and make foci that open up to form a ciliated lumen called Kupffer’s vesicle.\r\n\r\nWe find that interfering with the deposition of components of the extracellular matrix, or with the intracellular anchors of the cells to the matrix, impairs the polarisation of the DFC’s and leads to subsequent defects in lumen formation. In addition, we have developed a method to culture the DFCs ex vivo, allowing us to precisely manipulate the extracellular environment. The possibility of combining the genetic study of Kupffer’s vesicle formation in the live embryo with cell biological techniques in organ culture make this system uniquely relevant for studying the role of the ECM in polarisation during organogenesis.\r\n","lang":"eng"}],"type":"journal_article","volume":126,"oa_version":"None","date_updated":"2021-01-12T07:54:52Z","date_created":"2018-12-11T12:07:14Z","author":[{"full_name":"Soete, Gwen","first_name":"Gwen","last_name":"Soete"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"intvolume":" 126","publisher":"Elsevier","publication_status":"published","title":"The role of the extracellular matrix in Kupffer's vesicle formation in zebrafish","status":"public","year":"2009","_id":"4149","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"issue":"8","publist_id":"1953","abstract":[{"lang":"eng","text":"The tissues of a developing embryo are simultaneously patterned, moved and differentiated according to an exchange of information between their constituent cells. We argue that these complex self-organizing phenomena can only be fully understood with quantitative mathematical frameworks that allow specific hypotheses to be formulated and tested. The quantitative and dynamic imaging of growing embryos at the molecular, cellular and tissue level is the key experimental advance required to achieve this interaction between theory and experiment. Here we describe how mathematical modelling has become an invaluable method to integrate quantitative biological information across temporal and spatial scales, serving to connect the activity of regulatory molecules with the morphological development of organisms."}],"extern":"1","type":"journal_article","author":[{"first_name":"Andrew","last_name":"Oates","full_name":"Oates, Andrew"},{"full_name":"Gorfinkiel, Nicole","last_name":"Gorfinkiel","first_name":"Nicole"},{"last_name":"Gonzalez Gaitan","first_name":"Marcos","full_name":"Gonzalez Gaitan, Marcos"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"oa_version":"None","volume":10,"date_updated":"2021-01-12T07:54:59Z","date_created":"2018-12-11T12:07:20Z","year":"2009","_id":"4165","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":" 10","publisher":"Nature Publishing Group","status":"public","title":"Quantitative approaches in developmental biology","publication_status":"published","article_processing_charge":"No","month":"08","day":"01","doi":"10.1038/nrg2548","date_published":"2009-08-01T00:00:00Z","language":[{"iso":"eng"}],"citation":{"chicago":"Oates, Andrew, Nicole Gorfinkiel, Marcos Gonzalez Gaitan, and Carl-Philipp J Heisenberg. “Quantitative Approaches in Developmental Biology.” Nature Reviews Genetics. Nature Publishing Group, 2009. https://doi.org/10.1038/nrg2548.","short":"A. Oates, N. Gorfinkiel, M. Gonzalez Gaitan, C.-P.J. Heisenberg, Nature Reviews Genetics 10 (2009) 517–530.","mla":"Oates, Andrew, et al. “Quantitative Approaches in Developmental Biology.” Nature Reviews Genetics, vol. 10, no. 8, Nature Publishing Group, 2009, pp. 517–30, doi:10.1038/nrg2548.","apa":"Oates, A., Gorfinkiel, N., Gonzalez Gaitan, M., & Heisenberg, C.-P. J. (2009). Quantitative approaches in developmental biology. Nature Reviews Genetics. Nature Publishing Group. https://doi.org/10.1038/nrg2548","ieee":"A. Oates, N. Gorfinkiel, M. Gonzalez Gaitan, and C.-P. J. Heisenberg, “Quantitative approaches in developmental biology,” Nature Reviews Genetics, vol. 10, no. 8. Nature Publishing Group, pp. 517–530, 2009.","ista":"Oates A, Gorfinkiel N, Gonzalez Gaitan M, Heisenberg C-PJ. 2009. Quantitative approaches in developmental biology. Nature Reviews Genetics. 10(8), 517–530.","ama":"Oates A, Gorfinkiel N, Gonzalez Gaitan M, Heisenberg C-PJ. Quantitative approaches in developmental biology. Nature Reviews Genetics. 2009;10(8):517-530. doi:10.1038/nrg2548"},"publication":"Nature Reviews Genetics","page":"517 - 530"},{"publication_status":"published","title":"Regulation of planar cell polarity signalling by the prenylation pathway","status":"public","publisher":"Elsevier","intvolume":" 126","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"4192","year":"2009","date_created":"2018-12-11T12:07:30Z","date_updated":"2021-01-12T07:55:11Z","volume":126,"oa_version":"None","author":[{"full_name":"Kai, Masatake","first_name":"Masatake","last_name":"Kai"},{"full_name":"Buchan, Nina","last_name":"Buchan","first_name":"Nina"},{"full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Tada, Masazumi","last_name":"Tada","first_name":"Masazumi"}],"type":"journal_article","extern":"1","abstract":[{"text":"During vertebrate gastrulation, the body axis is established by a variety of co-ordinated and directed movements of cells. One of these movements is convergence and extension (CE), which is regulated by a non-canonical Wnt/planar cell polarity (PCP) pathway. From our forward genetic screen, we have identified 3-hydroxy-3-methyglutaryl-coenzyme A reductase 1b (hmgcr1b) gene as a dominant enhancer of the silberblick (slb)/wnt11 CE phenotype. hmgcr1b mutant embryos exhibit only very mild CE phenotype during gastrulation while showing a thicker yolk extension at pharyngula stages. Notably, abrogation of hmgcr1b also enhances the CE defects of other core PCP mutants/morphants. The prenylation pathway is one of branches downstream of HMGCR, and has been implicated for lipid modification at the C-terminus of proteins. To test the possibility that the prenylation pathway regulates activities of the PCP pathway, we abrogated farnesyl transferase (FT) or geranylgeranyl transferase (GGT) function using morpholinos on PCP mutant/morphant backgrounds. Consistent with the notion that FT preferentially performs lipid modification on to proteins with the CAAX motif including the core PCP protein Prickle (Pk), abrogation of FT, but not GGT, enhances the pk1a or pk1b morphant CE phenotype, suggesting the specif icity for targets of the prenylation enzymes.\r\n","lang":"eng"}],"publist_id":"1927","issue":"Supplement 1","page":"S132 - S132","publication":"Mechanisms of Development","citation":{"apa":"Kai, M., Buchan, N., Heisenberg, C.-P. J., & Tada, M. (2009). Regulation of planar cell polarity signalling by the prenylation pathway. Mechanisms of Development. Elsevier. https://doi.org/10.1016/j.mod.2009.06.269","ieee":"M. Kai, N. Buchan, C.-P. J. Heisenberg, and M. Tada, “Regulation of planar cell polarity signalling by the prenylation pathway,” Mechanisms of Development, vol. 126, no. Supplement 1. Elsevier, pp. S132–S132, 2009.","ista":"Kai M, Buchan N, Heisenberg C-PJ, Tada M. 2009. Regulation of planar cell polarity signalling by the prenylation pathway. Mechanisms of Development. 126(Supplement 1), S132–S132.","ama":"Kai M, Buchan N, Heisenberg C-PJ, Tada M. Regulation of planar cell polarity signalling by the prenylation pathway. Mechanisms of Development. 2009;126(Supplement 1):S132-S132. doi:10.1016/j.mod.2009.06.269","chicago":"Kai, Masatake, Nina Buchan, Carl-Philipp J Heisenberg, and Masazumi Tada. “Regulation of Planar Cell Polarity Signalling by the Prenylation Pathway.” Mechanisms of Development. Elsevier, 2009. https://doi.org/10.1016/j.mod.2009.06.269.","short":"M. Kai, N. Buchan, C.-P.J. Heisenberg, M. Tada, Mechanisms of Development 126 (2009) S132–S132.","mla":"Kai, Masatake, et al. “Regulation of Planar Cell Polarity Signalling by the Prenylation Pathway.” Mechanisms of Development, vol. 126, no. Supplement 1, Elsevier, 2009, pp. S132–S132, doi:10.1016/j.mod.2009.06.269."},"language":[{"iso":"eng"}],"date_published":"2009-08-05T00:00:00Z","doi":"10.1016/j.mod.2009.06.269","day":"05","month":"08","article_processing_charge":"No"},{"type":"journal_article","extern":"1","issue":"7","publist_id":"1976","abstract":[{"text":"The migration of single cells and epithelial sheets is of great importance for gastrulation and organ formation in developing embryos and, if misregulated, can have dire consequences e.g. during cancer metastasis. A keystone of cell migration is the regulation of adhesive contacts, which are dynamically assembled and disassembled via endocytosis. Here, we discuss some of the basic concepts about the function of endocytic trafficking during cell migration: transport of integrins from the cell rear to the leading edge in fibroblasts; confinement of signalling to the front of single cells by endocytic transport of growth factors; regulation of movement coherence in multicellular sheets by cadherin turnover; and shaping of extracellular chemokine gradients. Taken together, endocytosis enables migrating cells and tissues to dynamically modulate their adhesion and signalling, allowing them to efficiently migrate through their extracellular environment.","lang":"eng"}],"intvolume":" 10","publisher":"Wiley-Blackwell","publication_status":"published","title":"Trafficking and cell migration","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"4143","year":"2009","volume":10,"oa_version":"None","date_updated":"2021-01-12T07:54:49Z","date_created":"2018-12-11T12:07:12Z","author":[{"full_name":"Ulrich, Florian","last_name":"Ulrich","first_name":"Florian"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"article_processing_charge":"No","month":"05","day":"20","page":"811 - 818","citation":{"chicago":"Ulrich, Florian, and Carl-Philipp J Heisenberg. “Trafficking and Cell Migration.” Traffic. Wiley-Blackwell, 2009. https://doi.org/10.1111/j.1600-0854.2009.00929.x.","mla":"Ulrich, Florian, and Carl-Philipp J. Heisenberg. “Trafficking and Cell Migration.” Traffic, vol. 10, no. 7, Wiley-Blackwell, 2009, pp. 811–18, doi:10.1111/j.1600-0854.2009.00929.x.","short":"F. Ulrich, C.-P.J. Heisenberg, Traffic 10 (2009) 811–818.","ista":"Ulrich F, Heisenberg C-PJ. 2009. Trafficking and cell migration. Traffic. 10(7), 811–818.","apa":"Ulrich, F., & Heisenberg, C.-P. J. (2009). Trafficking and cell migration. Traffic. Wiley-Blackwell. https://doi.org/10.1111/j.1600-0854.2009.00929.x","ieee":"F. Ulrich and C.-P. J. Heisenberg, “Trafficking and cell migration,” Traffic, vol. 10, no. 7. Wiley-Blackwell, pp. 811–818, 2009.","ama":"Ulrich F, Heisenberg C-PJ. Trafficking and cell migration. Traffic. 2009;10(7):811-818. doi:10.1111/j.1600-0854.2009.00929.x"},"publication":"Traffic","language":[{"iso":"eng"}],"doi":"10.1111/j.1600-0854.2009.00929.x","date_published":"2009-05-20T00:00:00Z"},{"extern":"1","publist_id":"1959","issue":"Supplement 1","abstract":[{"lang":"eng","text":"While the function of patterning in organogenesis is being extensively studied, considerably less is known of reverse effects that organ formation imposes on patterning. In zebrafish, the Kupffer’s vesicle (KV) and parapineal (PP) are embryonic struc- tures that share mechanisms of organogenesis and whose func- tion is essential for normal patterning along the left–right axis. Early morphogenesis of KV and PP organs involve the compaction of progenitor cells into a tight cluster within which three-dimen- sional cellular rosettes are formed. Organisation into rosettes pre- cedes the detachment of progenitor cells from neighbouring tissue and thus represents a key step towards organ formation. Such morphogenetic event is essential for organ function and its disruption has profound effects on left–right patterning."}],"type":"journal_article","volume":126,"oa_version":"None","date_created":"2018-12-11T12:07:18Z","date_updated":"2021-01-12T07:54:57Z","author":[{"full_name":"Oteíza, Pablo","last_name":"Oteíza","first_name":"Pablo"},{"last_name":"Lemus","first_name":"Carmen","full_name":"Lemus, Carmen"},{"full_name":"Köppen, Mathias","last_name":"Köppen","first_name":"Mathias"},{"full_name":"Palma, Karina","last_name":"Palma","first_name":"Karina"},{"last_name":"Krieg","first_name":"Michael","full_name":"Krieg, Michael"},{"first_name":"Cristina","last_name":"Melo","full_name":"Melo, Cristina"},{"full_name":"Farias, Cecilia","first_name":"Cecilia","last_name":"Farias"},{"full_name":"Pulgar, Eduardo","last_name":"Pulgar","first_name":"Eduardo"},{"last_name":"Preibisch","first_name":"Steffen","full_name":"Preibisch, Steffen"},{"full_name":"Hartel, Steffen","first_name":"Steffen","last_name":"Hartel"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Concha, Miguel","first_name":"Miguel","last_name":"Concha"}],"intvolume":" 126","publisher":"Elsevier","status":"public","publication_status":"published","title":"Linking organ formation to left-right patterning in the embryonic zebrafish","acknowledgement":"Grant sponsors: HHMI, CONICYT (PBCT ACT47, PBCT Red6), ICM P04-048-F, EU FP6-2004-NEST-PATH EDCBNL, DAAD.","_id":"4160","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2009","article_processing_charge":"No","month":"08","day":"05","language":[{"iso":"eng"}],"date_published":"2009-08-05T00:00:00Z","doi":"10.1016/j.mod.2009.06.970","page":"S11 - S11","citation":{"ama":"Oteíza P, Lemus C, Köppen M, et al. Linking organ formation to left-right patterning in the embryonic zebrafish. Mechanisms of Development. 2009;126(Supplement 1):S11-S11. doi:10.1016/j.mod.2009.06.970","ista":"Oteíza P, Lemus C, Köppen M, Palma K, Krieg M, Melo C, Farias C, Pulgar E, Preibisch S, Hartel S, Heisenberg C-PJ, Concha M. 2009. Linking organ formation to left-right patterning in the embryonic zebrafish. Mechanisms of Development. 126(Supplement 1), S11–S11.","apa":"Oteíza, P., Lemus, C., Köppen, M., Palma, K., Krieg, M., Melo, C., … Concha, M. (2009). Linking organ formation to left-right patterning in the embryonic zebrafish. Mechanisms of Development. Elsevier. https://doi.org/10.1016/j.mod.2009.06.970","ieee":"P. Oteíza et al., “Linking organ formation to left-right patterning in the embryonic zebrafish,” Mechanisms of Development, vol. 126, no. Supplement 1. Elsevier, pp. S11–S11, 2009.","mla":"Oteíza, Pablo, et al. “Linking Organ Formation to Left-Right Patterning in the Embryonic Zebrafish.” Mechanisms of Development, vol. 126, no. Supplement 1, Elsevier, 2009, pp. S11–S11, doi:10.1016/j.mod.2009.06.970.","short":"P. Oteíza, C. Lemus, M. Köppen, K. Palma, M. Krieg, C. Melo, C. Farias, E. Pulgar, S. Preibisch, S. Hartel, C.-P.J. Heisenberg, M. Concha, Mechanisms of Development 126 (2009) S11–S11.","chicago":"Oteíza, Pablo, Carmen Lemus, Mathias Köppen, Karina Palma, Michael Krieg, Cristina Melo, Cecilia Farias, et al. “Linking Organ Formation to Left-Right Patterning in the Embryonic Zebrafish.” Mechanisms of Development. Elsevier, 2009. https://doi.org/10.1016/j.mod.2009.06.970."},"publication":"Mechanisms of Development"},{"author":[{"full_name":"Oteíza, Pablo","first_name":"Pablo","last_name":"Oteíza"},{"full_name":"Köppen, Mathias","first_name":"Mathias","last_name":"Köppen"},{"first_name":"Michael","last_name":"Krieg","full_name":"Krieg, Michael"},{"first_name":"Steffen","last_name":"Preibisch","full_name":"Preibisch, Steffen"},{"full_name":"Haertel, Steffen","last_name":"Haertel","first_name":"Steffen"},{"full_name":"Müller, Daniel","first_name":"Daniel","last_name":"Müller"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"first_name":"Miguel","last_name":"Concha","full_name":"Concha, Miguel"}],"oa_version":"None","volume":126,"date_updated":"2021-01-12T07:54:58Z","date_created":"2018-12-11T12:07:19Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"4162","year":"2009","publisher":"Elsevier","intvolume":" 126","title":"Wnt11/Pk1a-mediated planar cell polarity signalling orchestrates epithelial organ morphogenesis by regulating N-cadherin dependent cell adhesion forces","status":"public","publication_status":"published","publist_id":"1957","issue":"Supplement 1","abstract":[{"lang":"eng","text":"Organ formation requires the precise assembly of progenitor cells into a functional unit. Mechanical forces are likely to play a critical role in this process, but it is unclear how these are molecularly controlled during development. Here, we show that Wnt11/ Pk1a-mediated planar cell polarity (PCP) signalling coordinates formation of the zebrafish laterality organ (Kupffer’s vesicle, KV) by regulating adhesion forces between organ progenitor cells (the dorsal forerunner cells, DFCs)."}],"extern":"1","type":"journal_article","doi":"10.1016/j.mod.2009.06.098","date_published":"2009-08-05T00:00:00Z","language":[{"iso":"eng"}],"citation":{"chicago":"Oteíza, Pablo, Mathias Köppen, Michael Krieg, Steffen Preibisch, Steffen Haertel, Daniel Müller, Carl-Philipp J Heisenberg, and Miguel Concha. “Wnt11/Pk1a-Mediated Planar Cell Polarity Signalling Orchestrates Epithelial Organ Morphogenesis by Regulating N-Cadherin Dependent Cell Adhesion Forces.” Mechanisms of Development. Elsevier, 2009. https://doi.org/10.1016/j.mod.2009.06.098.","mla":"Oteíza, Pablo, et al. “Wnt11/Pk1a-Mediated Planar Cell Polarity Signalling Orchestrates Epithelial Organ Morphogenesis by Regulating N-Cadherin Dependent Cell Adhesion Forces.” Mechanisms of Development, vol. 126, no. Supplement 1, Elsevier, 2009, pp. S80–S80, doi:10.1016/j.mod.2009.06.098.","short":"P. Oteíza, M. Köppen, M. Krieg, S. Preibisch, S. Haertel, D. Müller, C.-P.J. Heisenberg, M. Concha, Mechanisms of Development 126 (2009) S80–S80.","ista":"Oteíza P, Köppen M, Krieg M, Preibisch S, Haertel S, Müller D, Heisenberg C-PJ, Concha M. 2009. Wnt11/Pk1a-mediated planar cell polarity signalling orchestrates epithelial organ morphogenesis by regulating N-cadherin dependent cell adhesion forces. Mechanisms of Development. 126(Supplement 1), S80–S80.","ieee":"P. Oteíza et al., “Wnt11/Pk1a-mediated planar cell polarity signalling orchestrates epithelial organ morphogenesis by regulating N-cadherin dependent cell adhesion forces,” Mechanisms of Development, vol. 126, no. Supplement 1. Elsevier, pp. S80–S80, 2009.","apa":"Oteíza, P., Köppen, M., Krieg, M., Preibisch, S., Haertel, S., Müller, D., … Concha, M. (2009). Wnt11/Pk1a-mediated planar cell polarity signalling orchestrates epithelial organ morphogenesis by regulating N-cadherin dependent cell adhesion forces. Mechanisms of Development. Elsevier. https://doi.org/10.1016/j.mod.2009.06.098","ama":"Oteíza P, Köppen M, Krieg M, et al. Wnt11/Pk1a-mediated planar cell polarity signalling orchestrates epithelial organ morphogenesis by regulating N-cadherin dependent cell adhesion forces. Mechanisms of Development. 2009;126(Supplement 1):S80-S80. doi:10.1016/j.mod.2009.06.098"},"publication":"Mechanisms of Development","page":"S80 - S80","article_processing_charge":"No","month":"08","day":"05"},{"status":"public","publication_status":"published","title":"Biology and physics of cell shape changes in development","intvolume":" 19","publisher":"Cell Press","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"4158","year":"2009","date_updated":"2021-01-12T07:54:56Z","date_created":"2018-12-11T12:07:17Z","volume":19,"oa_version":"None","author":[{"last_name":"Paluch","first_name":"Ewa","full_name":"Paluch, Ewa"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"type":"journal_article","extern":"1","abstract":[{"text":"Together with cell growth, division and death, changes in cell shape are of central importance for tissue morphogenesis during development. Cell shape is the product of a cell's material and active properties balanced by external forces. Control of cell shape, therefore, relies on both tight regulation of intracellular mechanics and the cell's physical interaction with its environment. In this review, we first discuss the biological and physical mechanisms of cell shape control. We next examine a number of develop mental processes in which cell shape change - either individually or in a coordinated manner - drives embryonic morphogenesis and discuss how cell shape is controlled in these processes. Finally, we emphasize that cell shape control during tissue morphogenesis can only be fully understood by using a combination of cellular, molecular, developmental and biophysical approaches.","lang":"eng"}],"publist_id":"1960","issue":"17","page":"R790 - R799","publication":"Current Biology","citation":{"short":"E. Paluch, C.-P.J. Heisenberg, Current Biology 19 (2009) R790–R799.","mla":"Paluch, Ewa, and Carl-Philipp J. Heisenberg. “Biology and Physics of Cell Shape Changes in Development.” Current Biology, vol. 19, no. 17, Cell Press, 2009, pp. R790–99, doi:10.1016/j.cub.2009.07.029.","chicago":"Paluch, Ewa, and Carl-Philipp J Heisenberg. “Biology and Physics of Cell Shape Changes in Development.” Current Biology. Cell Press, 2009. https://doi.org/10.1016/j.cub.2009.07.029.","ama":"Paluch E, Heisenberg C-PJ. Biology and physics of cell shape changes in development. Current Biology. 2009;19(17):R790-R799. doi:10.1016/j.cub.2009.07.029","ieee":"E. Paluch and C.-P. J. Heisenberg, “Biology and physics of cell shape changes in development,” Current Biology, vol. 19, no. 17. Cell Press, pp. R790–R799, 2009.","apa":"Paluch, E., & Heisenberg, C.-P. J. (2009). Biology and physics of cell shape changes in development. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2009.07.029","ista":"Paluch E, Heisenberg C-PJ. 2009. Biology and physics of cell shape changes in development. Current Biology. 19(17), R790–R799."},"language":[{"iso":"eng"}],"doi":"10.1016/j.cub.2009.07.029","date_published":"2009-09-15T00:00:00Z","month":"09","day":"15","article_processing_charge":"No"},{"month":"01","day":"20","article_processing_charge":"No","page":"4 - 6","publication":"Developmental Cell","citation":{"ista":"Paluch E, Heisenberg C-PJ. 2009. Chaos begets order: Asynchronous cell contractions drive epithelial morphogenesis. Developmental Cell. 16(1), 4–6.","apa":"Paluch, E., & Heisenberg, C.-P. J. (2009). Chaos begets order: Asynchronous cell contractions drive epithelial morphogenesis. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2008.12.011","ieee":"E. Paluch and C.-P. J. Heisenberg, “Chaos begets order: Asynchronous cell contractions drive epithelial morphogenesis,” Developmental Cell, vol. 16, no. 1. Cell Press, pp. 4–6, 2009.","ama":"Paluch E, Heisenberg C-PJ. Chaos begets order: Asynchronous cell contractions drive epithelial morphogenesis. Developmental Cell. 2009;16(1):4-6. doi:10.1016/j.devcel.2008.12.011","chicago":"Paluch, Ewa, and Carl-Philipp J Heisenberg. “Chaos Begets Order: Asynchronous Cell Contractions Drive Epithelial Morphogenesis.” Developmental Cell. Cell Press, 2009. https://doi.org/10.1016/j.devcel.2008.12.011.","mla":"Paluch, Ewa, and Carl-Philipp J. Heisenberg. “Chaos Begets Order: Asynchronous Cell Contractions Drive Epithelial Morphogenesis.” Developmental Cell, vol. 16, no. 1, Cell Press, 2009, pp. 4–6, doi:10.1016/j.devcel.2008.12.011.","short":"E. Paluch, C.-P.J. Heisenberg, Developmental Cell 16 (2009) 4–6."},"language":[{"iso":"eng"}],"date_published":"2009-01-20T00:00:00Z","doi":"10.1016/j.devcel.2008.12.011","type":"journal_article","extern":"1","abstract":[{"text":"Apical cell contraction triggers tissue folding and invagination in epithelia. During Drosophila gastrulation, ventral furrow formation was thought to be driven by smooth, purse-string-like constriction of an actomyosin belt underlying adherens junctions. Now Martin et al. report in Nature that ventral furrow formation is triggered by asynchronous pulsed contractions of the apical acto-myosin cortex in individual cells.","lang":"eng"}],"publist_id":"1961","issue":"1","status":"public","title":"Chaos begets order: Asynchronous cell contractions drive epithelial morphogenesis","publication_status":"published","publisher":"Cell Press","intvolume":" 16","year":"2009","_id":"4159","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2018-12-11T12:07:18Z","date_updated":"2021-01-12T07:54:56Z","oa_version":"None","volume":16,"author":[{"first_name":"Ewa","last_name":"Paluch","full_name":"Paluch, Ewa"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}]}]