[{"ddc":["570"],"type":"journal_article","day":"01","publication_status":"published","file_date_updated":"2025-07-31T08:00:33Z","_id":"20082","year":"2025","language":[{"iso":"eng"}],"page":"1258–1266","PlanS_conform":"1","publication":"Nature Immunology","title":"Migrating immune cells globally coordinate protrusive forces","corr_author":"1","external_id":{"pmid":["40664976"],"isi":["001529134300001"]},"volume":26,"publisher":"Springer Nature","citation":{"ama":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, et al. Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. 2025;26:1258–1266. doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>","short":"P. Dos Reis Rodrigues, M. Avellaneda Sarrió, N. Canigova, F.R. Gärtner, K. Vaahtomeri, M. Riedl, I. de Vries, J. Merrin, R. Hauschild, Y. Fukui, A. Juanes Garcia, M.K. Sixt, Nature Immunology 26 (2025) 1258–1266.","chicago":"Dos Reis Rodrigues, Patricia, Mario Avellaneda Sarrió, Nikola Canigova, Florian R Gärtner, Kari Vaahtomeri, Michael Riedl, Ingrid de Vries, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>.","ieee":"P. Dos Reis Rodrigues <i>et al.</i>, “Migrating immune cells globally coordinate protrusive forces,” <i>Nature Immunology</i>, vol. 26. Springer Nature, pp. 1258–1266, 2025.","ista":"Dos Reis Rodrigues P, Avellaneda Sarrió M, Canigova N, Gärtner FR, Vaahtomeri K, Riedl M, de Vries I, Merrin J, Hauschild R, Fukui Y, Juanes Garcia A, Sixt MK. 2025. Migrating immune cells globally coordinate protrusive forces. Nature Immunology. 26, 1258–1266.","mla":"Dos Reis Rodrigues, Patricia, et al. “Migrating Immune Cells Globally Coordinate Protrusive Forces.” <i>Nature Immunology</i>, vol. 26, Springer Nature, 2025, pp. 1258–1266, doi:<a href=\"https://doi.org/10.1038/s41590-025-02211-w\">10.1038/s41590-025-02211-w</a>.","apa":"Dos Reis Rodrigues, P., Avellaneda Sarrió, M., Canigova, N., Gärtner, F. R., Vaahtomeri, K., Riedl, M., … Sixt, M. K. (2025). Migrating immune cells globally coordinate protrusive forces. <i>Nature Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41590-025-02211-w\">https://doi.org/10.1038/s41590-025-02211-w</a>"},"pmid":1,"intvolume":"        26","doi":"10.1038/s41590-025-02211-w","date_updated":"2026-04-28T13:26:50Z","publication_identifier":{"issn":["1529-2908"],"eissn":["1529-2916"]},"abstract":[{"text":"Efficient immune responses rely on the capacity of leukocytes to traverse diverse and complex tissues. To meet such changing environmental conditions, leukocytes usually adopt an ameboid configuration, using their forward-positioned nucleus as a probe to identify and follow the path of least resistance among pre-existing pores. We show that, in dense environments where even the largest pores preclude free passage, leukocytes position their nucleus behind the centrosome and organelles. The local compression imposed on the cell body by its surroundings triggers assembly of a central F-actin pool, located between cell front and nucleus. Central actin pushes outward to transiently dilate a path for organelles and nucleus. Pools of central and front actin are tightly coupled and experimental depletion of the central pool enhances actin accumulation and protrusion formation at the cell front. Although this shifted balance speeds up cells in permissive environments, migration in restrictive environments is impaired, as the unleashed leading edge dissociates from the trapped cell body. Our findings establish an actin regulatory loop that balances path dilation with advancement of the leading edge to maintain cellular coherence.","lang":"eng"}],"article_processing_charge":"Yes (via OA deal)","isi":1,"OA_type":"hybrid","quality_controlled":"1","OA_place":"publisher","oa_version":"Published Version","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"article_type":"letter_note","month":"08","has_accepted_license":"1","author":[{"id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","orcid":"0000-0003-1681-508X","first_name":"Patricia","full_name":"Dos Reis Rodrigues, Patricia","last_name":"Dos Reis Rodrigues"},{"id":"DC4BA84C-56E6-11EA-AD5D-348C3DDC885E","orcid":"0000-0001-6406-524X","first_name":"Mario","last_name":"Avellaneda Sarrió","full_name":"Avellaneda Sarrió, Mario"},{"orcid":"0000-0002-8518-5926","id":"3795523E-F248-11E8-B48F-1D18A9856A87","full_name":"Canigova, Nikola","last_name":"Canigova","first_name":"Nikola"},{"orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","last_name":"Gärtner","first_name":"Florian R"},{"id":"368EE576-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7829-3518","first_name":"Kari","last_name":"Vaahtomeri","full_name":"Vaahtomeri, Kari"},{"id":"3BE60946-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4844-6311","first_name":"Michael","last_name":"Riedl","full_name":"Riedl, Michael"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","full_name":"De Vries, Ingrid","last_name":"De Vries"},{"orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert"},{"last_name":"Fukui","full_name":"Fukui, Yoshinori","first_name":"Yoshinori"},{"id":"40F05888-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1009-9652","first_name":"Alba","last_name":"Juanes Garcia","full_name":"Juanes Garcia, Alba"},{"last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"project":[{"_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces"},{"name":"Bioelectric patrolling: the role of the local membrane potential in immune cell migration","grant_number":"944-2020","_id":"c092d618-5a5b-11eb-8a69-f92e1e843fc8"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","acknowledgement":"This research was supported by the Scientific Service Units of ISTA through resources provided by the Imaging and Optics, Preclinical and Lab Support Facilities. In particular, we thank M. A. Symth and F. G. G. Leite, from the Virus Service Team, who helped generating the lentiviral particles used in this study. We thank all the members of the Sixt group for valuable discussions and feedback, in particular, I. Mayer, for helping with T cell isolation and Z. (P.) Li for providing the Actin–GFP DC line. We are also thankful to J. Mandl and C. Shen for their feedback during the writing of this manuscript. This work was supported by a European Research Council grant ERC-SyG 101071793 to M.S. M.J.A. was supported by an HFSP Postdoctoral Fellowship LTF 177 2021 and A.J.G. by a Lise Meitner Fellowship of the FWF (Austrian Science Fund). Y.F. was supported by the AMED-CREST (JP19gm1310005), the Medical Research Center Initiative for High Depth Omics and CURE:JPMXP1323015486 for MIB, Kyushu University. Open access funding provided by Institute of Science and Technology (IST Austria).","fulldoi":"https://doi.org/10.1038/s41590-025-02211-w","status":"public","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_published":"2025-08-01T00:00:00Z","related_material":{"link":[{"url":"https://ista.ac.at/en/news/bench-pressing-cells/","relation":"press_release","description":"News on ISTA website"}],"record":[{"relation":"dissertation_contains","status":"public","id":"20149"}]},"oa":1,"file":[{"date_updated":"2025-07-31T08:00:33Z","success":1,"file_size":13514646,"creator":"dernst","content_type":"application/pdf","file_id":"20096","file_name":"2025_NatureImmunology_ReisRodrigues.pdf","date_created":"2025-07-31T08:00:33Z","access_level":"open_access","relation":"main_file","checksum":"0c725123dca7797c682609bff2c4c5ac"}],"date_created":"2025-07-27T22:01:26Z","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}]},{"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2024-07-18T00:00:00Z","status":"public","fulldoi":"https://doi.org/10.1038/s41586-024-07671-y","acknowledgement":"We thank S. Helmer, N. Blount, E. Raatz and Z. Sisic for technical assistance. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) SFB 1123 (S.M. project B06); SFB 914 (S.M. projects B02 and Z01, H.I.-A. project Z01, S.S. project A06, K.S. project B02, C. Schulz project A10, B.W. project A02, C. Scheiermann project B09); SFB 1054 (T.B. project B03); FOR2033 (F.G., R.A.J.O., S.M.); Individual research grant project ID: 514478744 (F.G.); Heisenberg Programme project ID: 514477451 (F.G.); the DZHK (German Center for Cardiovascular Research) (MHA 1.4VD (S.M.), Postdoc Start-up Grant, 81×3600213 (F.G.)); and LMUexcellence NFF (F.G.). W.F. received funding from China Scholarship Council (CSC, no. 201306270012). P.B. is supported by the German Research Foundation (DFG, project IDs 322900939, 432698239 and 445703531), European Research Council (ERC Consolidator grant no. 101001791) and the Federal Ministry of Education and Research (BMBF, STOP-FSGS-01GM2202C and NATON within the framework of the Network of University Medicine, no. 01KX2121). S.v.S. is supported by the START-Program of the Faculty of Medicine of the RWTH Aachen University (AZ 125/17). A.D. and S.E. are supported by the German Research Foundation (SFB TRR 267); S.E. by the BMBF in the framework of the Cluster4future program (CNATM—Cluster for Nucleic Acid Therapeutics Munich). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 833440 to S.M.). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687. The project is funded by the European Union (ERC, MEKanics, 101078110). Views and opinions expressed are those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency. Neither the European Union nor the granting authority can be held responsible for them.","author":[{"orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner","full_name":"Gärtner, Florian R","first_name":"Florian R"},{"first_name":"Hellen","last_name":"Ishikawa-Ankerhold","full_name":"Ishikawa-Ankerhold, Hellen"},{"first_name":"Susanne","last_name":"Stutte","full_name":"Stutte, Susanne"},{"first_name":"Wenwen","last_name":"Fu","full_name":"Fu, Wenwen"},{"full_name":"Weitz, Jutta","last_name":"Weitz","first_name":"Jutta"},{"full_name":"Dueck, Anne","last_name":"Dueck","first_name":"Anne"},{"first_name":"Bhavishya","full_name":"Nelakuditi, Bhavishya","last_name":"Nelakuditi"},{"last_name":"Fumagalli","full_name":"Fumagalli, Valeria","first_name":"Valeria"},{"first_name":"Dominic","last_name":"Van Den Heuvel","full_name":"Van Den Heuvel, Dominic"},{"full_name":"Belz, Larissa","last_name":"Belz","first_name":"Larissa"},{"first_name":"Gulnoza","last_name":"Sobirova","full_name":"Sobirova, Gulnoza"},{"full_name":"Zhang, Zhe","last_name":"Zhang","first_name":"Zhe"},{"first_name":"Anna","full_name":"Titova, Anna","last_name":"Titova"},{"full_name":"Navarro, Alejandro Martinez","last_name":"Navarro","first_name":"Alejandro Martinez"},{"first_name":"Kami","full_name":"Pekayvaz, Kami","last_name":"Pekayvaz"},{"first_name":"Michael","last_name":"Lorenz","full_name":"Lorenz, Michael"},{"last_name":"Von Baumgarten","full_name":"Von Baumgarten, Louisa","first_name":"Louisa"},{"first_name":"Jan","last_name":"Kranich","full_name":"Kranich, Jan"},{"full_name":"Straub, Tobias","last_name":"Straub","first_name":"Tobias"},{"first_name":"Bastian","last_name":"Popper","full_name":"Popper, Bastian"},{"last_name":"Zheden","full_name":"Zheden, Vanessa","first_name":"Vanessa","orcid":"0000-0002-9438-4783","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Walter","last_name":"Kaufmann","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315"},{"last_name":"Guo","full_name":"Guo, Chenglong","first_name":"Chenglong"},{"full_name":"Piontek, Guido","last_name":"Piontek","first_name":"Guido"},{"full_name":"Von Stillfried, Saskia","last_name":"Von Stillfried","first_name":"Saskia"},{"first_name":"Peter","full_name":"Boor, Peter","last_name":"Boor"},{"first_name":"Marco","last_name":"Colonna","full_name":"Colonna, Marco"},{"first_name":"Sebastian","last_name":"Clauß","full_name":"Clauß, Sebastian"},{"full_name":"Schulz, Christian","last_name":"Schulz","first_name":"Christian"},{"first_name":"Thomas","full_name":"Brocker, Thomas","last_name":"Brocker"},{"full_name":"Walzog, Barbara","last_name":"Walzog","first_name":"Barbara"},{"last_name":"Scheiermann","full_name":"Scheiermann, Christoph","first_name":"Christoph"},{"first_name":"William C.","last_name":"Aird","full_name":"Aird, William C."},{"full_name":"Nerlov, Claus","last_name":"Nerlov","first_name":"Claus"},{"first_name":"Konstantin","full_name":"Stark, Konstantin","last_name":"Stark"},{"first_name":"Tobias","last_name":"Petzold","full_name":"Petzold, Tobias"},{"first_name":"Stefan","last_name":"Engelhardt","full_name":"Engelhardt, Stefan"},{"last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Rudelius, Martina","last_name":"Rudelius","first_name":"Martina"},{"last_name":"Oostendorp","full_name":"Oostendorp, Robert A.J.","first_name":"Robert A.J."},{"full_name":"Iannacone, Matteo","last_name":"Iannacone","first_name":"Matteo"},{"first_name":"Matthias","full_name":"Heinig, Matthias","last_name":"Heinig"},{"last_name":"Massberg","full_name":"Massberg, Steffen","first_name":"Steffen"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","project":[{"name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020","grant_number":"747687","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"date_created":"2024-07-21T22:01:02Z","related_material":{"link":[{"url":"https://github.com/heiniglab/gaertner_megakaryocytes","relation":"software"}]},"oa":1,"file":[{"file_size":15704819,"success":1,"date_updated":"2024-07-22T06:16:11Z","content_type":"application/pdf","creator":"dernst","file_name":"2024_Nature_Gaertner.pdf","file_id":"17286","relation":"main_file","checksum":"aa004afc72d2489f0fb0fcbc9919fbbd","access_level":"open_access","date_created":"2024-07-22T06:16:11Z"}],"article_type":"original","quality_controlled":"1","department":[{"_id":"EM-Fac"},{"_id":"MiSi"},{"_id":"Bio"}],"oa_version":"Published Version","isi":1,"article_processing_charge":"Yes (in subscription journal)","has_accepted_license":"1","month":"07","date_updated":"2025-09-08T08:14:25Z","doi":"10.1038/s41586-024-07671-y","citation":{"ama":"Gärtner FR, Ishikawa-Ankerhold H, Stutte S, et al. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. <i>Nature</i>. 2024;631:645-653. doi:<a href=\"https://doi.org/10.1038/s41586-024-07671-y\">10.1038/s41586-024-07671-y</a>","short":"F.R. Gärtner, H. Ishikawa-Ankerhold, S. Stutte, W. Fu, J. Weitz, A. Dueck, B. Nelakuditi, V. Fumagalli, D. Van Den Heuvel, L. Belz, G. Sobirova, Z. Zhang, A. Titova, A.M. Navarro, K. Pekayvaz, M. Lorenz, L. Von Baumgarten, J. Kranich, T. Straub, B. Popper, V. Zheden, W. Kaufmann, C. Guo, G. Piontek, S. Von Stillfried, P. Boor, M. Colonna, S. Clauß, C. Schulz, T. Brocker, B. Walzog, C. Scheiermann, W.C. Aird, C. Nerlov, K. Stark, T. Petzold, S. Engelhardt, M.K. Sixt, R. Hauschild, M. Rudelius, R.A.J. Oostendorp, M. Iannacone, M. Heinig, S. Massberg, Nature 631 (2024) 645–653.","chicago":"Gärtner, Florian R, Hellen Ishikawa-Ankerhold, Susanne Stutte, Wenwen Fu, Jutta Weitz, Anne Dueck, Bhavishya Nelakuditi, et al. “Plasmacytoid Dendritic Cells Control Homeostasis of Megakaryopoiesis.” <i>Nature</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41586-024-07671-y\">https://doi.org/10.1038/s41586-024-07671-y</a>.","ista":"Gärtner FR, Ishikawa-Ankerhold H, Stutte S, Fu W, Weitz J, Dueck A, Nelakuditi B, Fumagalli V, Van Den Heuvel D, Belz L, Sobirova G, Zhang Z, Titova A, Navarro AM, Pekayvaz K, Lorenz M, Von Baumgarten L, Kranich J, Straub T, Popper B, Zheden V, Kaufmann W, Guo C, Piontek G, Von Stillfried S, Boor P, Colonna M, Clauß S, Schulz C, Brocker T, Walzog B, Scheiermann C, Aird WC, Nerlov C, Stark K, Petzold T, Engelhardt S, Sixt MK, Hauschild R, Rudelius M, Oostendorp RAJ, Iannacone M, Heinig M, Massberg S. 2024. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. Nature. 631, 645–653.","ieee":"F. R. Gärtner <i>et al.</i>, “Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis,” <i>Nature</i>, vol. 631. Springer Nature, pp. 645–653, 2024.","mla":"Gärtner, Florian R., et al. “Plasmacytoid Dendritic Cells Control Homeostasis of Megakaryopoiesis.” <i>Nature</i>, vol. 631, Springer Nature, 2024, pp. 645–53, doi:<a href=\"https://doi.org/10.1038/s41586-024-07671-y\">10.1038/s41586-024-07671-y</a>.","apa":"Gärtner, F. R., Ishikawa-Ankerhold, H., Stutte, S., Fu, W., Weitz, J., Dueck, A., … Massberg, S. (2024). Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-024-07671-y\">https://doi.org/10.1038/s41586-024-07671-y</a>"},"pmid":1,"intvolume":"       631","abstract":[{"lang":"eng","text":"Platelet homeostasis is essential for vascular integrity and immune defence1,2. Although the process of platelet formation by fragmenting megakaryocytes (MKs; thrombopoiesis) has been extensively studied, the cellular and molecular mechanisms required to constantly replenish the pool of MKs by their progenitor cells (megakaryopoiesis) remains unclear3,4. Here we use intravital imaging to track the cellular dynamics of megakaryopoiesis over days. We identify plasmacytoid dendritic cells (pDCs) as homeostatic sensors that monitor the bone marrow for apoptotic MKs and deliver IFNα to the MK niche triggering local on-demand proliferation and maturation of MK progenitors. This pDC-dependent feedback loop is crucial for MK and platelet homeostasis at steady state and under stress. pDCs are best known for their ability to function as vigilant detectors of viral infection5. We show that virus-induced activation of pDCs interferes with their function as homeostatic sensors of megakaryopoiesis. Consequently, activation of pDCs by SARS-CoV-2 leads to excessive megakaryopoiesis. Together, we identify a pDC-dependent homeostatic circuit that involves innate immune sensing and demand-adapted release of inflammatory mediators to maintain homeostasis of the megakaryocytic lineage."}],"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"publication_status":"published","day":"18","type":"journal_article","ddc":["570"],"publisher":"Springer Nature","external_id":{"pmid":["38987596"],"isi":["001281636500020"]},"ec_funded":1,"volume":631,"publication":"Nature","title":"Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis","corr_author":"1","year":"2024","_id":"17284","file_date_updated":"2024-07-22T06:16:11Z","page":"645-653","language":[{"iso":"eng"}]},{"intvolume":"        57","pmid":1,"citation":{"mla":"Stark, Konstantin, et al. “Antibodies and Complement Are Key Drivers of Thrombosis.” <i>Immunity</i>, vol. 57, no. 9, Elsevier, 2024, pp. 2140–56, doi:<a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">10.1016/j.immuni.2024.08.007</a>.","apa":"Stark, K., Kilani, B., Stockhausen, S., Busse, J., Schubert, I., Tran, T. D., … Massberg, S. (2024). Antibodies and complement are key drivers of thrombosis. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">https://doi.org/10.1016/j.immuni.2024.08.007</a>","ama":"Stark K, Kilani B, Stockhausen S, et al. Antibodies and complement are key drivers of thrombosis. <i>Immunity</i>. 2024;57(9):2140-2156. doi:<a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">10.1016/j.immuni.2024.08.007</a>","short":"K. Stark, B. Kilani, S. Stockhausen, J. Busse, I. Schubert, T.D. Tran, F.R. Gärtner, A. Leunig, K. Pekayvaz, L. Nicolai, V. Fumagalli, J. Stermann, F. Stephan, C. David, M.B. Müller, B. Heyman, A. Lux, A. Da Palma Guerreiro, L.P. Frenzel, C.Q. Schmidt, A. Dopler, M. Moser, S. Chandraratne, M.L. Von Brühl, M. Lorenz, T. Korff, M. Rudelius, O. Popp, M. Kirchner, P. Mertins, F. Nimmerjahn, M. Iannacone, M. Sperandio, B. Engelmann, A. Verschoor, S. Massberg, Immunity 57 (2024) 2140–2156.","ieee":"K. Stark <i>et al.</i>, “Antibodies and complement are key drivers of thrombosis,” <i>Immunity</i>, vol. 57, no. 9. Elsevier, pp. 2140–2156, 2024.","ista":"Stark K, Kilani B, Stockhausen S, Busse J, Schubert I, Tran TD, Gärtner FR, Leunig A, Pekayvaz K, Nicolai L, Fumagalli V, Stermann J, Stephan F, David C, Müller MB, Heyman B, Lux A, Da Palma Guerreiro A, Frenzel LP, Schmidt CQ, Dopler A, Moser M, Chandraratne S, Von Brühl ML, Lorenz M, Korff T, Rudelius M, Popp O, Kirchner M, Mertins P, Nimmerjahn F, Iannacone M, Sperandio M, Engelmann B, Verschoor A, Massberg S. 2024. Antibodies and complement are key drivers of thrombosis. Immunity. 57(9), 2140–2156.","chicago":"Stark, Konstantin, Badr Kilani, Sven Stockhausen, Johanna Busse, Irene Schubert, Thuy Duong Tran, Florian R Gärtner, et al. “Antibodies and Complement Are Key Drivers of Thrombosis.” <i>Immunity</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.immuni.2024.08.007\">https://doi.org/10.1016/j.immuni.2024.08.007</a>."},"doi":"10.1016/j.immuni.2024.08.007","date_updated":"2025-09-08T09:50:13Z","issue":"9","publication_identifier":{"eissn":["1097-4180"]},"abstract":[{"text":"Venous thromboembolism (VTE) is a common, deadly disease with an increasing incidence despite preventive efforts. Clinical observations have associated elevated antibody concentrations or antibody-based therapies with thrombotic events. However, how antibodies contribute to thrombosis is unknown. Here, we show that reduced blood flow enabled immunoglobulin M (IgM) to bind to FcμR and the polymeric immunoglobulin receptor (pIgR), initiating endothelial activation and platelet recruitment. Subsequently, the procoagulant surface of activated platelets accommodated antigen- and FcγR-independent IgG deposition. This leads to classical complement activation, setting in motion a prothrombotic vicious circle. Key elements of this mechanism were present in humans in the setting of venous stasis as well as in the dysregulated immunothrombosis of COVID-19. This antibody-driven thrombosis can be prevented by pharmacologically targeting complement. Hence, our results uncover antibodies as previously unrecognized central regulators of thrombosis. These findings carry relevance for therapeutic application of antibodies and open innovative avenues to target thrombosis without compromising hemostasis.","lang":"eng"}],"ddc":["570"],"type":"journal_article","day":"10","publication_status":"published","page":"2140-2156","language":[{"iso":"eng"}],"_id":"18109","file_date_updated":"2024-09-30T09:16:03Z","year":"2024","title":"Antibodies and complement are key drivers of thrombosis","publication":"Immunity","volume":57,"external_id":{"pmid":["39226900"],"isi":["001317438500001"]},"publisher":"Elsevier","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","author":[{"first_name":"Konstantin","full_name":"Stark, Konstantin","last_name":"Stark"},{"first_name":"Badr","full_name":"Kilani, Badr","last_name":"Kilani"},{"full_name":"Stockhausen, Sven","last_name":"Stockhausen","first_name":"Sven"},{"full_name":"Busse, Johanna","last_name":"Busse","first_name":"Johanna"},{"full_name":"Schubert, Irene","last_name":"Schubert","first_name":"Irene"},{"full_name":"Tran, Thuy Duong","last_name":"Tran","first_name":"Thuy Duong"},{"first_name":"Florian R","last_name":"Gärtner","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723"},{"first_name":"Alexander","full_name":"Leunig, Alexander","last_name":"Leunig"},{"first_name":"Kami","last_name":"Pekayvaz","full_name":"Pekayvaz, Kami"},{"full_name":"Nicolai, Leo","last_name":"Nicolai","first_name":"Leo"},{"first_name":"Valeria","full_name":"Fumagalli, Valeria","last_name":"Fumagalli"},{"last_name":"Stermann","full_name":"Stermann, Julia","first_name":"Julia"},{"last_name":"Stephan","full_name":"Stephan, Felix","first_name":"Felix"},{"last_name":"David","full_name":"David, Christian","first_name":"Christian"},{"full_name":"Müller, Martin B.","last_name":"Müller","first_name":"Martin B."},{"full_name":"Heyman, Birgitta","last_name":"Heyman","first_name":"Birgitta"},{"full_name":"Lux, Anja","last_name":"Lux","first_name":"Anja"},{"first_name":"Alexandra","last_name":"Da Palma Guerreiro","full_name":"Da Palma Guerreiro, Alexandra"},{"first_name":"Lukas P.","last_name":"Frenzel","full_name":"Frenzel, Lukas P."},{"first_name":"Christoph Q.","full_name":"Schmidt, Christoph Q.","last_name":"Schmidt"},{"first_name":"Arthur","full_name":"Dopler, Arthur","last_name":"Dopler"},{"first_name":"Markus","full_name":"Moser, Markus","last_name":"Moser"},{"first_name":"Sue","last_name":"Chandraratne","full_name":"Chandraratne, Sue"},{"full_name":"Von Brühl, Marie Luise","last_name":"Von Brühl","first_name":"Marie Luise"},{"last_name":"Lorenz","full_name":"Lorenz, Michael","first_name":"Michael"},{"full_name":"Korff, Thomas","last_name":"Korff","first_name":"Thomas"},{"first_name":"Martina","full_name":"Rudelius, Martina","last_name":"Rudelius"},{"first_name":"Oliver","full_name":"Popp, Oliver","last_name":"Popp"},{"first_name":"Marieluise","full_name":"Kirchner, Marieluise","last_name":"Kirchner"},{"last_name":"Mertins","full_name":"Mertins, Philipp","first_name":"Philipp"},{"last_name":"Nimmerjahn","full_name":"Nimmerjahn, Falk","first_name":"Falk"},{"first_name":"Matteo","last_name":"Iannacone","full_name":"Iannacone, Matteo"},{"full_name":"Sperandio, Markus","last_name":"Sperandio","first_name":"Markus"},{"first_name":"Bernd","last_name":"Engelmann","full_name":"Engelmann, Bernd"},{"first_name":"Admar","last_name":"Verschoor","full_name":"Verschoor, Admar"},{"full_name":"Massberg, Steffen","last_name":"Massberg","first_name":"Steffen"}],"acknowledgement":"We thank Michael Carroll (Harvard Medical School, Boston) for providing Ighmtm1Che, C4−/−, and C3−/− mice; Mark Suter (University of Zurich, Zurich) for providing Aicda−/− mice; Marina Botto (Imperial College London, London) for providing C1q−/− and fB−/− mice; Craig Gerard (Harvard Medical School, Boston) for providing C3aR−/− mice; Falk Nimmerjahn (University Erlangen-Nuernberg, Erlangen) for providing Fcgr−/−Fcgr2b−/− mice; Karl Lang (University of Duisburg-Essen, Essen) for providing Fcmr−/− mice; Hans Hengartner and Rolf Zinkernagel (ETH Zurich, Zurich) for providing KL25 mice; Mark Zabel (University Hospital of Zurich, Zurich) for providing CR2−/− mice; Christie Ballantyne (Baylor College of Medicine, Houston) for providing CD11c−/− mice; and Siamon Gordon (University of Oxford, Oxford) for providing CD11b−/− mice. A.V. wishes to thank Michael Grünaug and dedicates this work to Annette, Rita, and Hans.\r\nThis project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. \r\n947611) (K.S.). This study was supported by the Deutsche Forschungsgemeinschaft through the collaborative research center 914 project B02 (K.S. and S.M.), project B04 (A.V.), project A01 (M.M.), project B01 (M.S.), the collaborative research center 1123 project B07 (K.S. and S.M.), the collaborative research center 359 (project A03 [K.S.] and B02 [M.S.]), the international research training group 1911 project B09 (A.V.), the clinical research unit 303 project 7 (A.V.), cluster of excellence 2167 (A.V.), collaborative research center 1526 project 05 (A.V.), the ANR-DFG project JAKPOT (K.S.), LMUexcellent (K.S.), and the Deutsche Zentrum für Herz-Kreislauf-Forschung (PostDoc Grant and partner site project [K.S. and S.M.]). M.I. is supported by the European Research Council (ERC) Advanced Grant 101141363, ERC Proof of Concept Grant 101138728, Italian Association for Cancer Research (AIRC) Grants 19891 and \r\n22737, Italian Ministry for University and Research Grants PE00000007 (INF-ACT) and PRIN \r\n2022FMESXL, Funded Research Agreement from Asher Biotherapeutics, VIR Biotechnology, and BlueJay Therapeutics. V.F. is supported by the Italian Ministry for University and Research Grants PE00000007 (INF-ACT) and Fondazione Prossimo Mio.","status":"public","fulldoi":"https://doi.org/10.1016/j.immuni.2024.08.007","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2024-09-10T00:00:00Z","oa":1,"file":[{"file_size":6892750,"success":1,"date_updated":"2024-09-30T09:16:03Z","content_type":"application/pdf","creator":"dernst","file_name":"2024_Immunity_Stark.pdf","file_id":"18162","checksum":"4683de43d06a8fd8e3fc91af4ddc1ba2","relation":"main_file","access_level":"open_access","date_created":"2024-09-30T09:16:03Z"}],"date_created":"2024-09-22T22:01:42Z","article_processing_charge":"Yes (in subscription journal)","isi":1,"oa_version":"Published Version","department":[{"_id":"MiSi"}],"quality_controlled":"1","article_type":"original","month":"09","has_accepted_license":"1"},{"citation":{"mla":"Nicolai, Leo, et al. “Single Platelet and Megakaryocyte Morpho-Dynamics Uncovered by Multicolor Reporter Mouse Strains in Vitro and in Vivo.” <i>Haematologica</i>, vol. 107, no. 7, Ferrata Storti Foundation, 2022, pp. 1669–80, doi:<a href=\"https://doi.org/10.3324/haematol.2021.278896\">10.3324/haematol.2021.278896</a>.","apa":"Nicolai, L., Kaiser, R., Escaig, R., Hoffknecht, M. L., Anjum, A., Leunig, A., … Gärtner, F. R. (2022). Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. <i>Haematologica</i>. Ferrata Storti Foundation. <a href=\"https://doi.org/10.3324/haematol.2021.278896\">https://doi.org/10.3324/haematol.2021.278896</a>","ama":"Nicolai L, Kaiser R, Escaig R, et al. Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. <i>Haematologica</i>. 2022;107(7):1669-1680. doi:<a href=\"https://doi.org/10.3324/haematol.2021.278896\">10.3324/haematol.2021.278896</a>","short":"L. Nicolai, R. Kaiser, R. Escaig, M.L. Hoffknecht, A. Anjum, A. Leunig, J. Pircher, A. Ehrlich, M. Lorenz, H. Ishikawa-Ankerhold, W.C. Aird, S. Massberg, F.R. Gärtner, Haematologica 107 (2022) 1669–1680.","ieee":"L. Nicolai <i>et al.</i>, “Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo,” <i>Haematologica</i>, vol. 107, no. 7. Ferrata Storti Foundation, pp. 1669–1680, 2022.","chicago":"Nicolai, Leo, Rainer Kaiser, Raphael Escaig, Marie Louise Hoffknecht, Afra Anjum, Alexander Leunig, Joachim Pircher, et al. “Single Platelet and Megakaryocyte Morpho-Dynamics Uncovered by Multicolor Reporter Mouse Strains in Vitro and in Vivo.” <i>Haematologica</i>. Ferrata Storti Foundation, 2022. <a href=\"https://doi.org/10.3324/haematol.2021.278896\">https://doi.org/10.3324/haematol.2021.278896</a>.","ista":"Nicolai L, Kaiser R, Escaig R, Hoffknecht ML, Anjum A, Leunig A, Pircher J, Ehrlich A, Lorenz M, Ishikawa-Ankerhold H, Aird WC, Massberg S, Gärtner FR. 2022. Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo. Haematologica. 107(7), 1669–1680."},"intvolume":"       107","doi":"10.3324/haematol.2021.278896","issue":"7","date_updated":"2025-04-14T07:43:16Z","publication_identifier":{"eissn":["1592-8721"],"issn":["0390-6078"]},"abstract":[{"lang":"eng","text":"Visualizing cell behavior and effector function on a single cell level has been crucial for understanding key aspects of mammalian biology. Due to their small size, large number and rapid recruitment into thrombi, there is a lack of data on fate and behavior of individual platelets in thrombosis and hemostasis. Here we report the use of platelet lineage restricted multi-color reporter mouse strains to delineate platelet function on a single cell level. We show that genetic labeling allows for single platelet and megakaryocyte (MK) tracking and morphological analysis in vivo and in vitro, while not affecting lineage functions. Using Cre-driven Confetti expression, we provide insights into temporal gene expression patterns as well as spatial clustering of MK in the bone marrow. In the vasculature, shape analysis of activated platelets recruited to thrombi identifies ubiquitous filopodia formation with no evidence of lamellipodia formation. Single cell tracking in complex thrombi reveals prominent myosin-dependent motility of platelets and highlights thrombus formation as a highly dynamic process amenable to modification and intervention of the acto-myosin cytoskeleton. Platelet function assays combining flow cytrometry, as well as in vivo, ex vivo and in vitro imaging show unaltered platelet functions of multicolor reporter mice compared to wild-type controls. In conclusion, platelet lineage multicolor reporter mice prove useful in furthering our understanding of platelet and MK biology on a single cell level."}],"ddc":["570"],"type":"journal_article","day":"01","publication_status":"published","_id":"11588","year":"2022","file_date_updated":"2022-07-18T07:51:55Z","page":"1669-1680","language":[{"iso":"eng"}],"publication":"Haematologica","title":"Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo","corr_author":"1","external_id":{"isi":["000823746100018"]},"ec_funded":1,"volume":107,"publisher":"Ferrata Storti Foundation","author":[{"first_name":"Leo","last_name":"Nicolai","full_name":"Nicolai, Leo"},{"first_name":"Rainer","full_name":"Kaiser, Rainer","last_name":"Kaiser"},{"first_name":"Raphael","full_name":"Escaig, Raphael","last_name":"Escaig"},{"last_name":"Hoffknecht","full_name":"Hoffknecht, Marie Louise","first_name":"Marie Louise"},{"first_name":"Afra","full_name":"Anjum, Afra","last_name":"Anjum"},{"full_name":"Leunig, Alexander","last_name":"Leunig","first_name":"Alexander"},{"first_name":"Joachim","full_name":"Pircher, Joachim","last_name":"Pircher"},{"first_name":"Andreas","full_name":"Ehrlich, Andreas","last_name":"Ehrlich"},{"first_name":"Michael","full_name":"Lorenz, Michael","last_name":"Lorenz"},{"first_name":"Hellen","full_name":"Ishikawa-Ankerhold, Hellen","last_name":"Ishikawa-Ankerhold"},{"first_name":"William C.","full_name":"Aird, William C.","last_name":"Aird"},{"last_name":"Massberg","full_name":"Massberg, Steffen","first_name":"Steffen"},{"first_name":"Florian R","full_name":"Gärtner, Florian R","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)"},"project":[{"call_identifier":"H2020","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687"}],"scopus_import":"1","acknowledgement":"This study was supported by the Deutsche Forschungsgemeinschaft (DFG) SFB 914 ( to SM [B02 and Z01]), the DFG SFB 1123 (to SM [B06]), the DFG FOR 2033 (to SM), the German\r\nCenter for Cardiovascular Research (DZHK) (Clinician Scientist Programme), MHA 1.4VD (to SM), Postdoc Start-up Grant, 81X3600213 (to FG), 81X3600222 (to LN), the FP7 program\r\n(project 260309, PRESTIGE [to SM]). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 83344, ERC-2018-ADG “IMMUNOTHROMBOSIS” [to SM] and the Marie Skłodowska Curie Individual Fellowship (EU project 747687, LamelliActin [to FG]). ","status":"public","fulldoi":"https://doi.org/10.3324/haematol.2021.278896","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_published":"2022-07-01T00:00:00Z","file":[{"file_name":"2022_Haematologica_Nicolai.pdf","file_id":"11595","relation":"main_file","checksum":"9b47830945f3c30428fe9cfee2dc4a8a","access_level":"open_access","date_created":"2022-07-18T07:51:55Z","file_size":1722094,"success":1,"date_updated":"2022-07-18T07:51:55Z","content_type":"application/pdf","creator":"dernst"}],"oa":1,"date_created":"2022-07-17T22:01:54Z","article_processing_charge":"No","isi":1,"quality_controlled":"1","oa_version":"Published Version","department":[{"_id":"MiSi"}],"article_type":"original","month":"07","has_accepted_license":"1"},{"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_published":"2022-12-13T00:00:00Z","status":"public","fulldoi":"https://doi.org/10.1016/j.immuni.2022.10.001","acknowledgement":"We thank Coung Kieu and Dominik van den Heuvel for excellent technical assistance. This work was supported by the German Research Foundation (PE2704/2-1, PE2704/3-1 to T.P., SFB 1123-project B06 to S.M., SFB1525 project A07 to D.S, TRR 332 project A7 to C.S., PO 2247/2-1 to A.P., SFB1116-project B11 to A.P. and B12 to M.K.), LMU Munich’s Institutional\r\nStrategy LMUexcellent within the framework of the German Excellence Initiative (No. 806 32 006 to T.P.), and by the German Centre for Cardiovascular Research (DZHK) to T.P. (Postdoc Start-up grant No. 100378833). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 833440 to S.M.). F.G. received funding from the European Union’s\r\nHorizon 2020 research and innovation program under the Marie Sk1odowska-Curie grant agreement no. 747687. A.H. was funded by RTI2018-095497-B-I00 from Ministerio de Ciencia e Innovacio´ n (MICINN), HR17_00527 from Fundacion La Caixa, and Transatlantic Network of Excellence (TNE-18CVD04) from the Leducq Foundation. The CNIC is supported by the MICINN and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (CEX2020-001041-S). A.P. was supported by the Forschungskommission of the Medical Faculty of the Heinrich-Heine-Universität Düsseldorf (No. 18-2019 to A.P.). C.G. was supported by the Helmholtz Alliance ‘Aging and Metabolic Programming, AMPro,’ by the German Federal\r\nMinistry of Education and Research to the German Center for Diabetes Research (DZD), and by the Bavarian State Ministry of Health and Care through the research project DigiMed Bayern.","author":[{"first_name":"Tobias","last_name":"Petzold","full_name":"Petzold, Tobias"},{"full_name":"Zhang, Zhe","last_name":"Zhang","first_name":"Zhe"},{"last_name":"Ballesteros","full_name":"Ballesteros, Iván","first_name":"Iván"},{"first_name":"Inas","last_name":"Saleh","full_name":"Saleh, Inas"},{"first_name":"Amin","full_name":"Polzin, Amin","last_name":"Polzin"},{"full_name":"Thienel, Manuela","last_name":"Thienel","first_name":"Manuela"},{"first_name":"Lulu","last_name":"Liu","full_name":"Liu, Lulu"},{"first_name":"Qurrat","last_name":"Ul Ain","full_name":"Ul Ain, Qurrat"},{"first_name":"Vincent","full_name":"Ehreiser, Vincent","last_name":"Ehreiser"},{"full_name":"Weber, Christian","last_name":"Weber","first_name":"Christian"},{"last_name":"Kilani","full_name":"Kilani, Badr","first_name":"Badr"},{"first_name":"Pontus","last_name":"Mertsch","full_name":"Mertsch, Pontus"},{"first_name":"Jeremias","last_name":"Götschke","full_name":"Götschke, Jeremias"},{"full_name":"Cremer, Sophie","last_name":"Cremer","first_name":"Sophie"},{"full_name":"Fu, Wenwen","last_name":"Fu","first_name":"Wenwen"},{"last_name":"Lorenz","full_name":"Lorenz, Michael","first_name":"Michael"},{"last_name":"Ishikawa-Ankerhold","full_name":"Ishikawa-Ankerhold, Hellen","first_name":"Hellen"},{"full_name":"Raatz, Elisabeth","last_name":"Raatz","first_name":"Elisabeth"},{"last_name":"El-Nemr","full_name":"El-Nemr, Shaza","first_name":"Shaza"},{"full_name":"Görlach, Agnes","last_name":"Görlach","first_name":"Agnes"},{"first_name":"Esther","full_name":"Marhuenda, Esther","last_name":"Marhuenda"},{"full_name":"Stark, Konstantin","last_name":"Stark","first_name":"Konstantin"},{"first_name":"Joachim","full_name":"Pircher, Joachim","last_name":"Pircher"},{"first_name":"David","last_name":"Stegner","full_name":"Stegner, David"},{"full_name":"Gieger, Christian","last_name":"Gieger","first_name":"Christian"},{"first_name":"Marc","full_name":"Schmidt-Supprian, Marc","last_name":"Schmidt-Supprian"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723","first_name":"Florian R","full_name":"Gärtner, Florian R","last_name":"Gärtner"},{"first_name":"Isaac","last_name":"Almendros","full_name":"Almendros, Isaac"},{"first_name":"Malte","full_name":"Kelm, Malte","last_name":"Kelm"},{"first_name":"Christian","full_name":"Schulz, Christian","last_name":"Schulz"},{"first_name":"Andrés","full_name":"Hidalgo, Andrés","last_name":"Hidalgo"},{"full_name":"Massberg, Steffen","last_name":"Massberg","first_name":"Steffen"}],"tmp":{"image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"scopus_import":"1","project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020"}],"date_created":"2023-01-12T11:56:54Z","file":[{"date_created":"2023-01-23T10:18:48Z","access_level":"open_access","checksum":"073267a9c0ad9f85a650053bc7b23777","relation":"main_file","file_id":"12341","file_name":"2022_Immunity_Petzold.pdf","creator":"dernst","content_type":"application/pdf","date_updated":"2023-01-23T10:18:48Z","success":1,"file_size":5299475}],"oa":1,"article_type":"original","quality_controlled":"1","department":[{"_id":"MiSi"}],"oa_version":"Published Version","isi":1,"keyword":["Infectious Diseases","Immunology","Immunology and Allergy"],"article_processing_charge":"No","has_accepted_license":"1","month":"12","date_updated":"2025-04-14T07:43:16Z","issue":"12","doi":"10.1016/j.immuni.2022.10.001","pmid":1,"citation":{"apa":"Petzold, T., Zhang, Z., Ballesteros, I., Saleh, I., Polzin, A., Thienel, M., … Massberg, S. (2022). Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">https://doi.org/10.1016/j.immuni.2022.10.001</a>","mla":"Petzold, Tobias, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>, vol. 55, no. 12, Elsevier, 2022, p. 2285–2299.e7, doi:<a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">10.1016/j.immuni.2022.10.001</a>.","chicago":"Petzold, Tobias, Zhe Zhang, Iván Ballesteros, Inas Saleh, Amin Polzin, Manuela Thienel, Lulu Liu, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">https://doi.org/10.1016/j.immuni.2022.10.001</a>.","ieee":"T. Petzold <i>et al.</i>, “Neutrophil ‘plucking’ on megakaryocytes drives platelet production and boosts cardiovascular disease,” <i>Immunity</i>, vol. 55, no. 12. Elsevier, p. 2285–2299.e7, 2022.","ista":"Petzold T, Zhang Z, Ballesteros I, Saleh I, Polzin A, Thienel M, Liu L, Ul Ain Q, Ehreiser V, Weber C, Kilani B, Mertsch P, Götschke J, Cremer S, Fu W, Lorenz M, Ishikawa-Ankerhold H, Raatz E, El-Nemr S, Görlach A, Marhuenda E, Stark K, Pircher J, Stegner D, Gieger C, Schmidt-Supprian M, Gärtner FR, Almendros I, Kelm M, Schulz C, Hidalgo A, Massberg S. 2022. Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. Immunity. 55(12), 2285–2299.e7.","ama":"Petzold T, Zhang Z, Ballesteros I, et al. Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. <i>Immunity</i>. 2022;55(12):2285-2299.e7. doi:<a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">10.1016/j.immuni.2022.10.001</a>","short":"T. Petzold, Z. Zhang, I. Ballesteros, I. Saleh, A. Polzin, M. Thienel, L. Liu, Q. Ul Ain, V. Ehreiser, C. Weber, B. Kilani, P. Mertsch, J. Götschke, S. Cremer, W. Fu, M. Lorenz, H. Ishikawa-Ankerhold, E. Raatz, S. El-Nemr, A. Görlach, E. Marhuenda, K. Stark, J. Pircher, D. Stegner, C. Gieger, M. Schmidt-Supprian, F.R. Gärtner, I. Almendros, M. Kelm, C. Schulz, A. Hidalgo, S. Massberg, Immunity 55 (2022) 2285–2299.e7."},"intvolume":"        55","abstract":[{"lang":"eng","text":"Intravascular neutrophils and platelets collaborate in maintaining host integrity, but their interaction can also trigger thrombotic complications. We report here that cooperation between neutrophil and platelet lineages extends to the earliest stages of platelet formation by megakaryocytes in the bone marrow. Using intravital microscopy, we show that neutrophils “plucked” intravascular megakaryocyte extensions, termed proplatelets, to control platelet production. Following CXCR4-CXCL12-dependent migration towards perisinusoidal megakaryocytes, plucking neutrophils actively pulled on proplatelets and triggered myosin light chain and extracellular-signal-regulated kinase activation through reactive oxygen species. By these mechanisms, neutrophils accelerate proplatelet growth and facilitate continuous release of platelets in steady state. Following myocardial infarction, plucking neutrophils drove excessive release of young, reticulated platelets and boosted the risk of recurrent ischemia. Ablation of neutrophil plucking normalized thrombopoiesis and reduced recurrent thrombosis after myocardial infarction and thrombus burden in venous thrombosis. We establish neutrophil plucking as a target to reduce thromboischemic events."}],"publication_identifier":{"issn":["1074-7613"]},"publication_status":"published","day":"13","type":"journal_article","ddc":["570"],"publisher":"Elsevier","external_id":{"isi":["000922019600003"],"pmid":["36272416"]},"volume":55,"ec_funded":1,"publication":"Immunity","title":"Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease","_id":"12119","file_date_updated":"2023-01-23T10:18:48Z","year":"2022","page":"2285-2299.e7","language":[{"iso":"eng"}]},{"month":"03","main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2021.03.002","open_access":"1"}],"article_processing_charge":"No","isi":1,"department":[{"_id":"MiSi"}],"oa_version":"Published Version","quality_controlled":"1","article_type":"original","oa":1,"date_created":"2021-03-28T22:01:41Z","scopus_import":"1","author":[{"first_name":"Florian R","last_name":"Gärtner","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723"},{"first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"}],"fulldoi":"https://doi.org/10.1016/j.devcel.2021.03.002","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2021-03-22T00:00:00Z","language":[{"iso":"eng"}],"page":"723-725","_id":"9294","year":"2021","title":"Engaging the front wheels to drive through fibrous terrain","corr_author":"1","publication":"Developmental Cell","volume":56,"external_id":{"pmid":["33756118"],"isi":["000631681200004"]},"publisher":"Elsevier","ddc":["570"],"type":"journal_article","day":"22","publication_status":"published","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"abstract":[{"lang":"eng","text":"In this issue of Developmental Cell, Doyle and colleagues identify periodic anterior contraction as a characteristic feature of fibroblasts and mesenchymal cancer cells embedded in 3D collagen gels. This contractile mechanism generates a matrix prestrain required for crawling in fibrous 3D environments."}],"intvolume":"        56","citation":{"apa":"Gärtner, F. R., &#38; Sixt, M. K. (2021). Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>","mla":"Gärtner, Florian R., and Michael K. Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>, vol. 56, no. 6, Elsevier, 2021, pp. 723–25, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>.","ieee":"F. R. Gärtner and M. K. Sixt, “Engaging the front wheels to drive through fibrous terrain,” <i>Developmental Cell</i>, vol. 56, no. 6. Elsevier, pp. 723–725, 2021.","ista":"Gärtner FR, Sixt MK. 2021. Engaging the front wheels to drive through fibrous terrain. Developmental Cell. 56(6), 723–725.","chicago":"Gärtner, Florian R, and Michael K Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>.","ama":"Gärtner FR, Sixt MK. Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. 2021;56(6):723-725. doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>","short":"F.R. Gärtner, M.K. Sixt, Developmental Cell 56 (2021) 723–725."},"pmid":1,"doi":"10.1016/j.devcel.2021.03.002","issue":"6","date_updated":"2026-06-18T19:45:10Z"},{"status":"public","fulldoi":"https://doi.org/10.1038/s41467-020-19515-0","date_published":"2020-11-13T00:00:00Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":"1","project":[{"grant_number":"747687","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells"}],"author":[{"last_name":"Nicolai","full_name":"Nicolai, Leo","first_name":"Leo"},{"first_name":"Karin","full_name":"Schiefelbein, Karin","last_name":"Schiefelbein"},{"full_name":"Lipsky, Silvia","last_name":"Lipsky","first_name":"Silvia"},{"first_name":"Alexander","last_name":"Leunig","full_name":"Leunig, Alexander"},{"full_name":"Hoffknecht, Marie","last_name":"Hoffknecht","first_name":"Marie"},{"last_name":"Pekayvaz","full_name":"Pekayvaz, Kami","first_name":"Kami"},{"first_name":"Ben","full_name":"Raude, Ben","last_name":"Raude"},{"first_name":"Charlotte","last_name":"Marx","full_name":"Marx, Charlotte"},{"first_name":"Andreas","last_name":"Ehrlich","full_name":"Ehrlich, Andreas"},{"full_name":"Pircher, Joachim","last_name":"Pircher","first_name":"Joachim"},{"first_name":"Zhe","last_name":"Zhang","full_name":"Zhang, Zhe"},{"full_name":"Saleh, Inas","last_name":"Saleh","first_name":"Inas"},{"full_name":"Marel, Anna-Kristina","last_name":"Marel","first_name":"Anna-Kristina"},{"full_name":"Löf, Achim","last_name":"Löf","first_name":"Achim"},{"last_name":"Petzold","full_name":"Petzold, Tobias","first_name":"Tobias"},{"first_name":"Michael","full_name":"Lorenz, Michael","last_name":"Lorenz"},{"first_name":"Konstantin","last_name":"Stark","full_name":"Stark, Konstantin"},{"first_name":"Robert","full_name":"Pick, Robert","last_name":"Pick"},{"last_name":"Rosenberger","full_name":"Rosenberger, Gerhild","first_name":"Gerhild"},{"first_name":"Ludwig","last_name":"Weckbach","full_name":"Weckbach, Ludwig"},{"last_name":"Uhl","full_name":"Uhl, Bernd","first_name":"Bernd"},{"full_name":"Xia, Sheng","last_name":"Xia","first_name":"Sheng"},{"first_name":"Christoph Andreas","last_name":"Reichel","full_name":"Reichel, Christoph Andreas"},{"first_name":"Barbara","last_name":"Walzog","full_name":"Walzog, Barbara"},{"full_name":"Schulz, Christian","last_name":"Schulz","first_name":"Christian"},{"full_name":"Zheden, Vanessa","last_name":"Zheden","first_name":"Vanessa","orcid":"0000-0002-9438-4783","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Markus","full_name":"Bender, Markus","last_name":"Bender"},{"last_name":"Li","full_name":"Li, Rong","first_name":"Rong"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"},{"first_name":"Florian R","last_name":"Gärtner","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723"}],"acknowledgement":"We thank Sebastian Helmer, Nicole Blount, Christine Mann, and Beate Jantz for technical assistance; Hellen Ishikawa-Ankerhold for help and advice; Michael Sixt for critical\r\ndiscussions. This study was supported by the DFG SFB 914 (S.M. [B02 and Z01], K.Sch.\r\n[B02], B.W. [A02 and Z03], C.A.R. [B03], C.S. [A10], J.P. [Gerok position]), the DFG\r\nSFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and F.G.), the German Center for\r\nCardiovascular Research (DZHK) (Clinician Scientist Program [L.N.], MHA 1.4VD\r\n[S.M.], Postdoc Start-up Grant, 81×3600213 [F.G.]), FP7 program (project 260309,\r\nPRESTIGE [S.M.]), FöFoLe project 1015/1009 (L.N.), FöFoLe project 947 (F.G.), the\r\nFriedrich-Baur-Stiftung project 41/16 (F.G.), and LMUexcellence NFF (F.G.). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no.\r\n833440) (S.M.). F.G. received funding from the European Union’s Horizon 2020 research\r\nand innovation program under the Marie Skłodowska-Curie grant agreement no.\r\n747687.","oa":1,"file":[{"checksum":"485b7b6cf30198ba0ce126491a28f125","relation":"main_file","access_level":"open_access","date_created":"2020-11-23T13:29:49Z","file_name":"2020_NatureComm_Nicolai.pdf","file_id":"8798","content_type":"application/pdf","creator":"dernst","file_size":7035340,"success":1,"date_updated":"2020-11-23T13:29:49Z"}],"related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-022-31310-7","relation":"erratum"}]},"date_created":"2020-11-22T23:01:23Z","department":[{"_id":"MiSi"},{"_id":"EM-Fac"}],"oa_version":"Published Version","quality_controlled":"1","article_type":"original","article_processing_charge":"No","isi":1,"has_accepted_license":"1","month":"11","doi":"10.1038/s41467-020-19515-0","date_updated":"2026-04-02T11:48:21Z","intvolume":"        11","citation":{"ista":"Nicolai L, Schiefelbein K, Lipsky S, Leunig A, Hoffknecht M, Pekayvaz K, Raude B, Marx C, Ehrlich A, Pircher J, Zhang Z, Saleh I, Marel A-K, Löf A, Petzold T, Lorenz M, Stark K, Pick R, Rosenberger G, Weckbach L, Uhl B, Xia S, Reichel CA, Walzog B, Schulz C, Zheden V, Bender M, Li R, Massberg S, Gärtner FR. 2020. Vascular surveillance by haptotactic blood platelets in inflammation and infection. Nature Communications. 11, 5778.","chicago":"Nicolai, Leo, Karin Schiefelbein, Silvia Lipsky, Alexander Leunig, Marie Hoffknecht, Kami Pekayvaz, Ben Raude, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>.","ieee":"L. Nicolai <i>et al.</i>, “Vascular surveillance by haptotactic blood platelets in inflammation and infection,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","short":"L. Nicolai, K. Schiefelbein, S. Lipsky, A. Leunig, M. Hoffknecht, K. Pekayvaz, B. Raude, C. Marx, A. Ehrlich, J. Pircher, Z. Zhang, I. Saleh, A.-K. Marel, A. Löf, T. Petzold, M. Lorenz, K. Stark, R. Pick, G. Rosenberger, L. Weckbach, B. Uhl, S. Xia, C.A. Reichel, B. Walzog, C. Schulz, V. Zheden, M. Bender, R. Li, S. Massberg, F.R. Gärtner, Nature Communications 11 (2020).","ama":"Nicolai L, Schiefelbein K, Lipsky S, et al. Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>","apa":"Nicolai, L., Schiefelbein, K., Lipsky, S., Leunig, A., Hoffknecht, M., Pekayvaz, K., … Gärtner, F. R. (2020). Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>","mla":"Nicolai, Leo, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>, vol. 11, 5778, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>."},"pmid":1,"abstract":[{"text":"Breakdown of vascular barriers is a major complication of inflammatory diseases. Anucleate platelets form blood-clots during thrombosis, but also play a crucial role in inflammation. While spatio-temporal dynamics of clot formation are well characterized, the cell-biological mechanisms of platelet recruitment to inflammatory micro-environments remain incompletely understood. Here we identify Arp2/3-dependent lamellipodia formation as a prominent morphological feature of immune-responsive platelets. Platelets use lamellipodia to scan for fibrin(ogen) deposited on the inflamed vasculature and to directionally spread, to polarize and to govern haptotactic migration along gradients of the adhesive ligand. Platelet-specific abrogation of Arp2/3 interferes with haptotactic repositioning of platelets to microlesions, thus impairing vascular sealing and provoking inflammatory microbleeding. During infection, haptotaxis promotes capture of bacteria and prevents hematogenic dissemination, rendering platelets gate-keepers of the inflamed microvasculature. Consequently, these findings identify haptotaxis as a key effector function of immune-responsive platelets.","lang":"eng"}],"publication_identifier":{"eissn":["2041-1723"]},"article_number":"5778","day":"13","publication_status":"published","ddc":["570"],"type":"journal_article","ec_funded":1,"volume":11,"external_id":{"isi":["000594648000014"],"pmid":["33188196"]},"publisher":"Springer Nature","language":[{"iso":"eng"}],"file_date_updated":"2020-11-23T13:29:49Z","_id":"8787","year":"2020","title":"Vascular surveillance by haptotactic blood platelets in inflammation and infection","corr_author":"1","publication":"Nature Communications"},{"abstract":[{"lang":"eng","text":"Eukaryotic cells migrate by coupling the intracellular force of the actin cytoskeleton to the environment. While force coupling is usually mediated by transmembrane adhesion receptors, especially those of the integrin family, amoeboid cells such as leukocytes can migrate extremely fast despite very low adhesive forces1. Here we show that leukocytes cannot only migrate under low adhesion but can also transmit forces in the complete absence of transmembrane force coupling. When confined within three-dimensional environments, they use the topographical features of the substrate to propel themselves. Here the retrograde flow of the actin cytoskeleton follows the texture of the substrate, creating retrograde shear forces that are sufficient to drive the cell body forwards. Notably, adhesion-dependent and adhesion-independent migration are not mutually exclusive, but rather are variants of the same principle of coupling retrograde actin flow to the environment and thus can potentially operate interchangeably and simultaneously. As adhesion-free migration is independent of the chemical composition of the environment, it renders cells completely autonomous in their locomotive behaviour."}],"publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"doi":"10.1038/s41586-020-2283-z","date_updated":"2026-09-22T22:30:16Z","pmid":1,"citation":{"mla":"Reversat, Anne, et al. “Cellular Locomotion Using Environmental Topography.” <i>Nature</i>, vol. 582, Springer Nature, 2020, pp. 582–585, doi:<a href=\"https://doi.org/10.1038/s41586-020-2283-z\">10.1038/s41586-020-2283-z</a>.","apa":"Reversat, A., Gärtner, F. R., Merrin, J., Stopp, J. A., Tasciyan, S., Aguilera Servin, J. L., … Sixt, M. K. (2020). Cellular locomotion using environmental topography. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-020-2283-z\">https://doi.org/10.1038/s41586-020-2283-z</a>","ama":"Reversat A, Gärtner FR, Merrin J, et al. Cellular locomotion using environmental topography. <i>Nature</i>. 2020;582:582–585. doi:<a href=\"https://doi.org/10.1038/s41586-020-2283-z\">10.1038/s41586-020-2283-z</a>","short":"A. Reversat, F.R. Gärtner, J. Merrin, J.A. Stopp, S. Tasciyan, J.L. Aguilera Servin, I. de Vries, R. Hauschild, M. Hons, M. Piel, A. Callan-Jones, R. Voituriez, M.K. Sixt, Nature 582 (2020) 582–585.","ieee":"A. Reversat <i>et al.</i>, “Cellular locomotion using environmental topography,” <i>Nature</i>, vol. 582. Springer Nature, pp. 582–585, 2020.","ista":"Reversat A, Gärtner FR, Merrin J, Stopp JA, Tasciyan S, Aguilera Servin JL, de Vries I, Hauschild R, Hons M, Piel M, Callan-Jones A, Voituriez R, Sixt MK. 2020. Cellular locomotion using environmental topography. Nature. 582, 582–585.","chicago":"Reversat, Anne, Florian R Gärtner, Jack Merrin, Julian A Stopp, Saren Tasciyan, Juan L Aguilera Servin, Ingrid de Vries, et al. “Cellular Locomotion Using Environmental Topography.” <i>Nature</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41586-020-2283-z\">https://doi.org/10.1038/s41586-020-2283-z</a>."},"intvolume":"       582","external_id":{"isi":["000532688300008"],"pmid":["32581372"]},"volume":582,"ec_funded":1,"publisher":"Springer Nature","_id":"7885","year":"2020","page":"582–585","language":[{"iso":"eng"}],"publication":"Nature","title":"Cellular locomotion using environmental topography","day":"25","publication_status":"published","type":"journal_article","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"related_material":{"record":[{"relation":"dissertation_contains","id":"12401","status":"public"},{"relation":"dissertation_contains","id":"14697","status":"public"}],"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/off-road-mode-enables-mobile-cells-to-move-freely/"}]},"oa":1,"date_created":"2020-05-24T22:01:01Z","status":"public","fulldoi":"https://doi.org/10.1038/s41586-020-2283-z","date_published":"2020-06-25T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Reversat, Anne","last_name":"Reversat","first_name":"Anne","orcid":"0000-0003-0666-8928","id":"35B76592-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Gärtner","full_name":"Gärtner, Florian R","first_name":"Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack"},{"id":"489E3F00-F248-11E8-B48F-1D18A9856A87","first_name":"Julian A","last_name":"Stopp","full_name":"Stopp, Julian A"},{"last_name":"Tasciyan","full_name":"Tasciyan, Saren","first_name":"Saren","orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Aguilera Servin, Juan L","last_name":"Aguilera Servin","first_name":"Juan L","orcid":"0000-0002-2862-8372","id":"2A67C376-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ingrid","full_name":"De Vries, Ingrid","last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert"},{"orcid":"0000-0002-6625-3348","id":"4167FE56-F248-11E8-B48F-1D18A9856A87","last_name":"Hons","full_name":"Hons, Miroslav","first_name":"Miroslav"},{"first_name":"Matthieu","full_name":"Piel, Matthieu","last_name":"Piel"},{"first_name":"Andrew","full_name":"Callan-Jones, Andrew","last_name":"Callan-Jones"},{"first_name":"Raphael","last_name":"Voituriez","full_name":"Voituriez, Raphael"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt"}],"project":[{"grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes"},{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373","name":"Cellular Navigation Along Spatial Gradients","call_identifier":"H2020"},{"name":"Mechanical adaptation of lamellipodial actin","call_identifier":"FWF","_id":"26018E70-B435-11E9-9278-68D0E5697425","grant_number":"P29911"},{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020"}],"scopus_import":"1","acknowledgement":"We thank A. Leithner and J. Renkawitz for discussion and critical reading of the manuscript; J. Schwarz and M. Mehling for establishing the microfluidic setups; the Bioimaging Facility of IST Austria for excellent support, as well as the Life Science Facility and the Miba Machine Shop of IST Austria; and F. N. Arslan, L. E. Burnett and L. Li for their work during their rotation in the IST PhD programme. This work was supported by the European Research Council (ERC StG 281556 and CoG 724373) to M.S. and grants from the Austrian Science Fund (FWF P29911) and the WWTF to M.S. M.H. was supported by the European Regional Development Fund Project (CZ.02.1.01/0.0/0.0/15_003/0000476). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687.","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/793919"}],"month":"06","OA_place":"repository","quality_controlled":"1","OA_type":"green","department":[{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"MiSi"}],"oa_version":"Preprint","article_type":"original","article_processing_charge":"No","isi":1},{"date_created":"2019-08-20T17:24:32Z","author":[{"full_name":"Gärtner, Florian R","last_name":"Gärtner","first_name":"Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Massberg, Steffen","last_name":"Massberg","first_name":"Steffen"}],"scopus_import":"1","project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020"}],"fulldoi":"https://doi.org/10.1038/s41577-019-0202-z","status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_published":"2019-12-01T00:00:00Z","month":"12","article_processing_charge":"No","isi":1,"quality_controlled":"1","oa_version":"None","department":[{"_id":"MiSi"}],"article_type":"original","publication_identifier":{"issn":["1474-1733"],"eissn":["1474-1741"]},"abstract":[{"text":"Platelets are small anucleate cellular fragments that are released by megakaryocytes and safeguard vascular integrity through a process termed ‘haemostasis’. However, platelets have important roles beyond haemostasis as they contribute to the initiation and coordination of intravascular immune responses. They continuously monitor blood vessel integrity and tightly coordinate vascular trafficking and functions of multiple cell types. In this way platelets act as ‘patrolling officers of the vascular highway’ that help to establish effective immune responses to infections and cancer. Here we discuss the distinct biological features of platelets that allow them to shape immune responses to pathogens and tumour cells, highlighting the parallels between these responses.","lang":"eng"}],"pmid":1,"citation":{"ieee":"F. R. Gärtner and S. Massberg, “Patrolling the vascular borders: Platelets in immunity to infection and cancer,” <i>Nature Reviews Immunology</i>, vol. 19, no. 12. Springer Nature, pp. 747–760, 2019.","ista":"Gärtner FR, Massberg S. 2019. Patrolling the vascular borders: Platelets in immunity to infection and cancer. Nature Reviews Immunology. 19(12), 747–760.","chicago":"Gärtner, Florian R, and Steffen Massberg. “Patrolling the Vascular Borders: Platelets in Immunity to Infection and Cancer.” <i>Nature Reviews Immunology</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41577-019-0202-z\">https://doi.org/10.1038/s41577-019-0202-z</a>.","ama":"Gärtner FR, Massberg S. Patrolling the vascular borders: Platelets in immunity to infection and cancer. <i>Nature Reviews Immunology</i>. 2019;19(12):747–760. doi:<a href=\"https://doi.org/10.1038/s41577-019-0202-z\">10.1038/s41577-019-0202-z</a>","short":"F.R. Gärtner, S. Massberg, Nature Reviews Immunology 19 (2019) 747–760.","apa":"Gärtner, F. R., &#38; Massberg, S. (2019). Patrolling the vascular borders: Platelets in immunity to infection and cancer. <i>Nature Reviews Immunology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41577-019-0202-z\">https://doi.org/10.1038/s41577-019-0202-z</a>","mla":"Gärtner, Florian R., and Steffen Massberg. “Patrolling the Vascular Borders: Platelets in Immunity to Infection and Cancer.” <i>Nature Reviews Immunology</i>, vol. 19, no. 12, Springer Nature, 2019, pp. 747–760, doi:<a href=\"https://doi.org/10.1038/s41577-019-0202-z\">10.1038/s41577-019-0202-z</a>."},"intvolume":"        19","doi":"10.1038/s41577-019-0202-z","date_updated":"2025-04-14T07:43:17Z","issue":"12","_id":"6824","year":"2019","language":[{"iso":"eng"}],"page":"747–760","publication":"Nature Reviews Immunology","title":"Patrolling the vascular borders: Platelets in immunity to infection and cancer","external_id":{"isi":["000499090600011"],"pmid":["31409920"]},"ec_funded":1,"volume":19,"publisher":"Springer Nature","type":"journal_article","day":"01","publication_status":"published"},{"day":"01","publication_status":"published","type":"journal_article","ec_funded":1,"volume":40,"external_id":{"isi":["000493292100005"],"pmid":["31601520"]},"publisher":"Cell Press","page":"922-938","language":[{"iso":"eng"}],"year":"2019","_id":"6988","title":"Platelets in host defense: Experimental and clinical insights","publication":"Trends in Immunology","doi":"10.1016/j.it.2019.08.004","issue":"10","date_updated":"2025-04-14T07:43:17Z","intvolume":"        40","citation":{"short":"L. Nicolai, F.R. Gärtner, S. Massberg, Trends in Immunology 40 (2019) 922–938.","ama":"Nicolai L, Gärtner FR, Massberg S. Platelets in host defense: Experimental and clinical insights. <i>Trends in Immunology</i>. 2019;40(10):922-938. doi:<a href=\"https://doi.org/10.1016/j.it.2019.08.004\">10.1016/j.it.2019.08.004</a>","ieee":"L. Nicolai, F. R. Gärtner, and S. Massberg, “Platelets in host defense: Experimental and clinical insights,” <i>Trends in Immunology</i>, vol. 40, no. 10. Cell Press, pp. 922–938, 2019.","chicago":"Nicolai, Leo, Florian R Gärtner, and Steffen Massberg. “Platelets in Host Defense: Experimental and Clinical Insights.” <i>Trends in Immunology</i>. Cell Press, 2019. <a href=\"https://doi.org/10.1016/j.it.2019.08.004\">https://doi.org/10.1016/j.it.2019.08.004</a>.","ista":"Nicolai L, Gärtner FR, Massberg S. 2019. Platelets in host defense: Experimental and clinical insights. Trends in Immunology. 40(10), 922–938.","mla":"Nicolai, Leo, et al. “Platelets in Host Defense: Experimental and Clinical Insights.” <i>Trends in Immunology</i>, vol. 40, no. 10, Cell Press, 2019, pp. 922–38, doi:<a href=\"https://doi.org/10.1016/j.it.2019.08.004\">10.1016/j.it.2019.08.004</a>.","apa":"Nicolai, L., Gärtner, F. R., &#38; Massberg, S. (2019). Platelets in host defense: Experimental and clinical insights. <i>Trends in Immunology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.it.2019.08.004\">https://doi.org/10.1016/j.it.2019.08.004</a>"},"pmid":1,"abstract":[{"lang":"eng","text":"Platelets are central players in thrombosis and hemostasis but are increasingly recognized as key components of the immune system. They shape ensuing immune responses by recruiting leukocytes, and support the development of adaptive immunity. Recent data shed new light on the complex role of platelets in immunity. Here, we summarize experimental and clinical data on the role of platelets in host defense against bacteria. Platelets bind, contain, and kill bacteria directly; however, platelet proinflammatory effector functions and cross-talk with the coagulation system, can also result in damage to the host (e.g., acute lung injury and sepsis). Novel clinical insights support this dichotomy: platelet inhibition/thrombocytopenia can be either harmful or protective, depending on pathophysiological context. Clinical studies are currently addressing this aspect in greater depth."}],"publication_identifier":{"issn":["1471-4906"]},"oa_version":"None","department":[{"_id":"MiSi"}],"quality_controlled":"1","article_type":"review","article_processing_charge":"No","isi":1,"month":"10","status":"public","fulldoi":"https://doi.org/10.1016/j.it.2019.08.004","date_published":"2019-10-01T00:00:00Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","project":[{"grant_number":"747687","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020"}],"scopus_import":"1","author":[{"last_name":"Nicolai","full_name":"Nicolai, Leo","first_name":"Leo"},{"last_name":"Gärtner","full_name":"Gärtner, Florian R","first_name":"Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Steffen","last_name":"Massberg","full_name":"Massberg, Steffen"}],"date_created":"2019-11-04T16:27:36Z"},{"date_created":"2019-04-29T09:40:33Z","oa":1,"file":[{"file_size":2928337,"date_updated":"2020-07-14T12:47:28Z","content_type":"application/pdf","creator":"dernst","file_id":"6360","file_name":"2018_BioProtocol_Fan.pdf","checksum":"d4588377e789da7f360b553ae02c5119","relation":"main_file","date_created":"2019-04-30T08:04:33Z","access_level":"open_access"}],"DOAJ_listed":"1","acknowledgement":"This protocol was adapted from a previously published study (Gaertner et al., 2017). We thank Michael Lorenz for his excellent assistance in bacteria culture. This work was funded by the DFG SFB 914 (S.M. [B02 and Z01]), the DFG SFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and F.G.), the German Centre for Cardiovascular Research (DZHK) (MHA 1.4VD [S.M.]), FP7 program (project 260309, PRESTIGE [S.M.]), FöFoLe project 947 (F.G.), the Friedrich-Baur-Stiftung project 41/16 (F.G.), Marie Sklodowska Curie Individual Fellowship (EU project 747687, LamelliaActin [F.G.]).","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"project":[{"grant_number":"747687","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020"}],"author":[{"full_name":"Fan, Shuxia","last_name":"Fan","first_name":"Shuxia"},{"first_name":"Michael","last_name":"Lorenz","full_name":"Lorenz, Michael"},{"last_name":"Massberg","full_name":"Massberg, Steffen","first_name":"Steffen"},{"orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","last_name":"Gärtner","first_name":"Florian R"}],"date_published":"2018-09-20T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","fulldoi":"https://doi.org/10.21769/bioprotoc.3018","month":"09","has_accepted_license":"1","article_processing_charge":"Yes","keyword":["Platelets","Cell migration","Bacteria","Shear flow","Fibrinogen","E. coli"],"article_type":"original","oa_version":"Published Version","department":[{"_id":"MiSi"}],"quality_controlled":"1","OA_type":"gold","OA_place":"publisher","publication_identifier":{"issn":["2331-8325"]},"article_number":"e3018","abstract":[{"text":"Blood platelets are critical for hemostasis and thrombosis, but also play diverse roles during immune responses. We have recently reported that platelets migrate at sites of infection in vitro and in vivo. Importantly, platelets use their ability to migrate to collect and bundle fibrin (ogen)-bound bacteria accomplishing efficient intravascular bacterial trapping. Here, we describe a method that allows analyzing platelet migration in vitro, focusing on their ability to collect bacteria and trap bacteria under flow.","lang":"eng"}],"intvolume":"         8","pmid":1,"citation":{"mla":"Fan, Shuxia, et al. “Platelet Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>, vol. 8, no. 18, e3018, Bio-Protocol, 2018, doi:<a href=\"https://doi.org/10.21769/bioprotoc.3018\">10.21769/bioprotoc.3018</a>.","apa":"Fan, S., Lorenz, M., Massberg, S., &#38; Gärtner, F. R. (2018). Platelet migration and bacterial trapping assay under flow. <i>Bio-Protocol</i>. Bio-Protocol. <a href=\"https://doi.org/10.21769/bioprotoc.3018\">https://doi.org/10.21769/bioprotoc.3018</a>","short":"S. Fan, M. Lorenz, S. Massberg, F.R. Gärtner, Bio-Protocol 8 (2018).","ama":"Fan S, Lorenz M, Massberg S, Gärtner FR. Platelet migration and bacterial trapping assay under flow. <i>Bio-Protocol</i>. 2018;8(18). doi:<a href=\"https://doi.org/10.21769/bioprotoc.3018\">10.21769/bioprotoc.3018</a>","ieee":"S. Fan, M. Lorenz, S. Massberg, and F. R. Gärtner, “Platelet migration and bacterial trapping assay under flow,” <i>Bio-Protocol</i>, vol. 8, no. 18. Bio-Protocol, 2018.","chicago":"Fan, Shuxia, Michael Lorenz, Steffen Massberg, and Florian R Gärtner. “Platelet Migration and Bacterial Trapping Assay under Flow.” <i>Bio-Protocol</i>. Bio-Protocol, 2018. <a href=\"https://doi.org/10.21769/bioprotoc.3018\">https://doi.org/10.21769/bioprotoc.3018</a>.","ista":"Fan S, Lorenz M, Massberg S, Gärtner FR. 2018. Platelet migration and bacterial trapping assay under flow. Bio-Protocol. 8(18), e3018."},"issue":"18","date_updated":"2025-05-20T07:43:06Z","doi":"10.21769/bioprotoc.3018","corr_author":"1","title":"Platelet migration and bacterial trapping assay under flow","publication":"Bio-Protocol","language":[{"iso":"eng"}],"year":"2018","_id":"6354","file_date_updated":"2020-07-14T12:47:28Z","publisher":"Bio-Protocol","volume":8,"ec_funded":1,"external_id":{"pmid":["34395806"]},"type":"journal_article","ddc":["570"],"publication_status":"published","day":"20"},{"publisher":"Nature Publishing Group","ec_funded":1,"volume":19,"external_id":{"isi":["000433041500026"],"pmid":["29777221"]},"title":"Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells","publication":"Nature Immunology","language":[{"iso":"eng"}],"page":"606 - 616","_id":"15","year":"2018","publication_status":"published","day":"18","type":"journal_article","ddc":["570"],"publist_id":"8040","abstract":[{"text":"Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together with analysis of cytoskeletal dynamics in vitro to deconstruct the basic mechanisms of T cell migration within lymphatic organs. We show that the contributions of the integrin LFA-1 and the chemokine receptor CCR7 are complementary rather than positioned in a linear pathway, as they are during leukocyte extravasation from the blood vasculature. Our data demonstrate that CCR7 controls cortical actin flows, whereas integrins mediate substrate friction that is sufficient to drive locomotion in the absence of considerable surface adhesions and plasma membrane flux.","lang":"eng"}],"issue":"6","date_updated":"2026-09-22T22:30:24Z","doi":"10.1038/s41590-018-0109-z","intvolume":"        19","pmid":1,"citation":{"ama":"Hons M, Kopf A, Hauschild R, et al. Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. <i>Nature Immunology</i>. 2018;19(6):606-616. doi:<a href=\"https://doi.org/10.1038/s41590-018-0109-z\">10.1038/s41590-018-0109-z</a>","short":"M. Hons, A. Kopf, R. Hauschild, A.F. Leithner, F.R. Gärtner, J. Abe, J. Renkawitz, J. Stein, M.K. Sixt, Nature Immunology 19 (2018) 606–616.","ieee":"M. Hons <i>et al.</i>, “Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells,” <i>Nature Immunology</i>, vol. 19, no. 6. Nature Publishing Group, pp. 606–616, 2018.","chicago":"Hons, Miroslav, Aglaja Kopf, Robert Hauschild, Alexander F Leithner, Florian R Gärtner, Jun Abe, Jörg Renkawitz, Jens Stein, and Michael K Sixt. “Chemokines and Integrins Independently Tune Actin Flow and Substrate Friction during Intranodal Migration of T Cells.” <i>Nature Immunology</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41590-018-0109-z\">https://doi.org/10.1038/s41590-018-0109-z</a>.","ista":"Hons M, Kopf A, Hauschild R, Leithner AF, Gärtner FR, Abe J, Renkawitz J, Stein J, Sixt MK. 2018. Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nature Immunology. 19(6), 606–616.","mla":"Hons, Miroslav, et al. “Chemokines and Integrins Independently Tune Actin Flow and Substrate Friction during Intranodal Migration of T Cells.” <i>Nature Immunology</i>, vol. 19, no. 6, Nature Publishing Group, 2018, pp. 606–16, doi:<a href=\"https://doi.org/10.1038/s41590-018-0109-z\">10.1038/s41590-018-0109-z</a>.","apa":"Hons, M., Kopf, A., Hauschild, R., Leithner, A. F., Gärtner, F. R., Abe, J., … Sixt, M. K. (2018). Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. <i>Nature Immunology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41590-018-0109-z\">https://doi.org/10.1038/s41590-018-0109-z</a>"},"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/29777221","open_access":"1"}],"month":"05","oa_version":"Published Version","department":[{"_id":"MiSi"},{"_id":"Bio"}],"quality_controlled":"1","isi":1,"article_processing_charge":"No","acknowledged_ssus":[{"_id":"SSU"}],"date_created":"2018-12-11T11:44:10Z","oa":1,"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"6891"}]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2018-05-18T00:00:00Z","status":"public","fulldoi":"https://doi.org/10.1038/s41590-018-0109-z","acknowledgement":"This work was funded by grants from the European Research Council (ERC StG 281556 and CoG 724373) and the Austrian Science Foundation (FWF) to M.S. and by Swiss National Foundation (SNF) project grants 31003A_135649, 31003A_153457 and CR23I3_156234 to J.V.S. F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687, and J.R. was funded by an EMBO long-term fellowship (ALTF 1396-2014).","scopus_import":"1","project":[{"grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Cellular Navigation Along Spatial Gradients"},{"call_identifier":"H2020","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687"},{"_id":"25A48D24-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 1396-2014","name":"Molecular and system level view of immune cell migration"},{"call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556"}],"author":[{"orcid":"0000-0002-6625-3348","id":"4167FE56-F248-11E8-B48F-1D18A9856A87","full_name":"Hons, Miroslav","last_name":"Hons","first_name":"Miroslav"},{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2187-6656","first_name":"Aglaja","last_name":"Kopf","full_name":"Kopf, Aglaja"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1073-744X","first_name":"Alexander F","last_name":"Leithner","full_name":"Leithner, Alexander F"},{"first_name":"Florian R","full_name":"Gärtner, Florian R","last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723"},{"last_name":"Abe","full_name":"Abe, Jun","first_name":"Jun"},{"last_name":"Renkawitz","full_name":"Renkawitz, Jörg","first_name":"Jörg","orcid":"0000-0003-2856-3369","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Stein, Jens","last_name":"Stein","first_name":"Jens"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K"}]},{"month":"11","isi":1,"article_processing_charge":"No","quality_controlled":"1","department":[{"_id":"MiSi"}],"oa_version":"None","date_created":"2018-12-11T11:47:15Z","author":[{"last_name":"Gärtner","full_name":"Gärtner, Florian R","first_name":"Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ahmad","full_name":"Ahmad, Zerkah","first_name":"Zerkah"},{"first_name":"Gerhild","full_name":"Rosenberger, Gerhild","last_name":"Rosenberger"},{"last_name":"Fan","full_name":"Fan, Shuxia","first_name":"Shuxia"},{"full_name":"Nicolai, Leo","last_name":"Nicolai","first_name":"Leo"},{"full_name":"Busch, Benjamin","last_name":"Busch","first_name":"Benjamin"},{"first_name":"Gökce","full_name":"Yavuz, Gökce","last_name":"Yavuz"},{"last_name":"Luckner","full_name":"Luckner, Manja","first_name":"Manja"},{"full_name":"Ishikawa Ankerhold, Hellen","last_name":"Ishikawa Ankerhold","first_name":"Hellen"},{"full_name":"Hennel, Roman","last_name":"Hennel","first_name":"Roman"},{"first_name":"Alexandre","last_name":"Benechet","full_name":"Benechet, Alexandre"},{"last_name":"Lorenz","full_name":"Lorenz, Michael","first_name":"Michael"},{"first_name":"Sue","full_name":"Chandraratne, Sue","last_name":"Chandraratne"},{"first_name":"Irene","last_name":"Schubert","full_name":"Schubert, Irene"},{"last_name":"Helmer","full_name":"Helmer, Sebastian","first_name":"Sebastian"},{"first_name":"Bianca","last_name":"Striednig","full_name":"Striednig, Bianca"},{"first_name":"Konstantin","last_name":"Stark","full_name":"Stark, Konstantin"},{"first_name":"Marek","full_name":"Janko, Marek","last_name":"Janko"},{"full_name":"Böttcher, Ralph","last_name":"Böttcher","first_name":"Ralph"},{"full_name":"Verschoor, Admar","last_name":"Verschoor","first_name":"Admar"},{"first_name":"Catherine","full_name":"Leon, Catherine","last_name":"Leon"},{"last_name":"Gachet","full_name":"Gachet, Christian","first_name":"Christian"},{"first_name":"Thomas","last_name":"Gudermann","full_name":"Gudermann, Thomas"},{"last_name":"Mederos Y Schnitzler","full_name":"Mederos Y Schnitzler, Michael","first_name":"Michael"},{"last_name":"Pincus","full_name":"Pincus, Zachary","first_name":"Zachary"},{"first_name":"Matteo","last_name":"Iannacone","full_name":"Iannacone, Matteo"},{"full_name":"Haas, Rainer","last_name":"Haas","first_name":"Rainer"},{"last_name":"Wanner","full_name":"Wanner, Gerhard","first_name":"Gerhard"},{"first_name":"Kirsten","full_name":"Lauber, Kirsten","last_name":"Lauber"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"}],"project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687","call_identifier":"H2020","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells"}],"scopus_import":"1","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2017-11-30T00:00:00Z","status":"public","fulldoi":"https://doi.org/10.1016/j.cell.2017.11.001","publication":"Cell Press","title":"Migrating platelets are mechano scavengers that collect and bundle bacteria","_id":"571","year":"2017","page":"1368 - 1382","language":[{"iso":"eng"}],"publisher":"Cell Press","external_id":{"isi":["000417362700018"]},"ec_funded":1,"volume":171,"type":"journal_article","publication_status":"published","day":"30","publication_identifier":{"issn":["0092-8674"]},"publist_id":"7243","abstract":[{"lang":"eng","text":"Blood platelets are critical for hemostasis and thrombosis and play diverse roles during immune responses. Despite these versatile tasks in mammalian biology, their skills on a cellular level are deemed limited, mainly consisting in rolling, adhesion, and aggregate formation. Here, we identify an unappreciated asset of platelets and show that adherent platelets use adhesion receptors to mechanically probe the adhesive substrate in their local microenvironment. When actomyosin-dependent traction forces overcome substrate resistance, platelets migrate and pile up the adhesive substrate together with any bound particulate material. They use this ability to act as cellular scavengers, scanning the vascular surface for potential invaders and collecting deposited bacteria. Microbe collection by migrating platelets boosts the activity of professional phagocytes, exacerbating inflammatory tissue injury in sepsis. This assigns platelets a central role in innate immune responses and identifies them as potential targets to dampen inflammatory tissue damage in clinical scenarios of severe systemic infection. In addition to their role in thrombosis and hemostasis, platelets can also migrate to sites of infection to help trap bacteria and clear the vascular surface."}],"citation":{"mla":"Gärtner, Florian R., et al. “Migrating Platelets Are Mechano Scavengers That Collect and Bundle Bacteria.” <i>Cell Press</i>, vol. 171, no. 6, Cell Press, 2017, pp. 1368–82, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">10.1016/j.cell.2017.11.001</a>.","apa":"Gärtner, F. R., Ahmad, Z., Rosenberger, G., Fan, S., Nicolai, L., Busch, B., … Massberg, S. (2017). Migrating platelets are mechano scavengers that collect and bundle bacteria. <i>Cell Press</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">https://doi.org/10.1016/j.cell.2017.11.001</a>","short":"F.R. Gärtner, Z. Ahmad, G. Rosenberger, S. Fan, L. Nicolai, B. Busch, G. Yavuz, M. Luckner, H. Ishikawa Ankerhold, R. Hennel, A. Benechet, M. Lorenz, S. Chandraratne, I. Schubert, S. Helmer, B. Striednig, K. Stark, M. Janko, R. Böttcher, A. Verschoor, C. Leon, C. Gachet, T. Gudermann, M. Mederos Y Schnitzler, Z. Pincus, M. Iannacone, R. Haas, G. Wanner, K. Lauber, M.K. Sixt, S. Massberg, Cell Press 171 (2017) 1368–1382.","ama":"Gärtner FR, Ahmad Z, Rosenberger G, et al. Migrating platelets are mechano scavengers that collect and bundle bacteria. <i>Cell Press</i>. 2017;171(6):1368-1382. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">10.1016/j.cell.2017.11.001</a>","ieee":"F. R. Gärtner <i>et al.</i>, “Migrating platelets are mechano scavengers that collect and bundle bacteria,” <i>Cell Press</i>, vol. 171, no. 6. Cell Press, pp. 1368–1382, 2017.","chicago":"Gärtner, Florian R, Zerkah Ahmad, Gerhild Rosenberger, Shuxia Fan, Leo Nicolai, Benjamin Busch, Gökce Yavuz, et al. “Migrating Platelets Are Mechano Scavengers That Collect and Bundle Bacteria.” <i>Cell Press</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.11.001\">https://doi.org/10.1016/j.cell.2017.11.001</a>.","ista":"Gärtner FR, Ahmad Z, Rosenberger G, Fan S, Nicolai L, Busch B, Yavuz G, Luckner M, Ishikawa Ankerhold H, Hennel R, Benechet A, Lorenz M, Chandraratne S, Schubert I, Helmer S, Striednig B, Stark K, Janko M, Böttcher R, Verschoor A, Leon C, Gachet C, Gudermann T, Mederos Y Schnitzler M, Pincus Z, Iannacone M, Haas R, Wanner G, Lauber K, Sixt MK, Massberg S. 2017. Migrating platelets are mechano scavengers that collect and bundle bacteria. Cell Press. 171(6), 1368–1382."},"intvolume":"       171","date_updated":"2025-09-11T07:39:45Z","issue":"6","doi":"10.1016/j.cell.2017.11.001"},{"date_created":"2018-12-11T12:06:06Z","extern":1,"publist_id":"2171","intvolume":"         7","author":[{"first_name":"Julia","full_name":"Riedl, Julia","last_name":"Riedl"},{"last_name":"Flynn","full_name":"Flynn, Kevin C","first_name":"Kevin"},{"last_name":"Raducanu","full_name":"Raducanu, Aurelia","first_name":"Aurelia"},{"full_name":"Florian Gärtner","last_name":"Gärtner","first_name":"Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Gisela","full_name":"Beck, Gisela","last_name":"Beck"},{"first_name":"Michael","last_name":"Bosl","full_name":"Bosl, Michael"},{"last_name":"Bradke","full_name":"Bradke, Frank","first_name":"Frank"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"},{"last_name":"Aszodi","full_name":"Aszodi, Attila","first_name":"Attila"},{"first_name":"Michael K","full_name":"Michael Sixt","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"first_name":"Roland","last_name":"Wedlich Söldner","full_name":"Wedlich-Söldner, Roland"}],"citation":{"mla":"Riedl, Julia, et al. “Lifeact Mice for Studying F-Actin Dynamics.” <i>Nature Methods</i>, vol. 7, no. 3, Nature Publishing Group, 2010, pp. 168–69, doi:<a href=\"https://doi.org/10.1038/nmeth0310-168\">10.1038/nmeth0310-168</a>.","apa":"Riedl, J., Flynn, K., Raducanu, A., Gärtner, F. R., Beck, G., Bosl, M., … Wedlich Söldner, R. (2010). Lifeact mice for studying F-actin dynamics. <i>Nature Methods</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nmeth0310-168\">https://doi.org/10.1038/nmeth0310-168</a>","ama":"Riedl J, Flynn K, Raducanu A, et al. Lifeact mice for studying F-actin dynamics. <i>Nature Methods</i>. 2010;7(3):168-169. doi:<a href=\"https://doi.org/10.1038/nmeth0310-168\">10.1038/nmeth0310-168</a>","short":"J. Riedl, K. Flynn, A. Raducanu, F.R. Gärtner, G. Beck, M. Bosl, F. Bradke, S. Massberg, A. Aszodi, M.K. Sixt, R. Wedlich Söldner, Nature Methods 7 (2010) 168–169.","chicago":"Riedl, Julia, Kevin Flynn, Aurelia Raducanu, Florian R Gärtner, Gisela Beck, Michael Bosl, Frank Bradke, et al. “Lifeact Mice for Studying F-Actin Dynamics.” <i>Nature Methods</i>. Nature Publishing Group, 2010. <a href=\"https://doi.org/10.1038/nmeth0310-168\">https://doi.org/10.1038/nmeth0310-168</a>.","ista":"Riedl J, Flynn K, Raducanu A, Gärtner FR, Beck G, Bosl M, Bradke F, Massberg S, Aszodi A, Sixt MK, Wedlich Söldner R. 2010. Lifeact mice for studying F-actin dynamics. Nature Methods. 7(3), 168–169.","ieee":"J. Riedl <i>et al.</i>, “Lifeact mice for studying F-actin dynamics,” <i>Nature Methods</i>, vol. 7, no. 3. Nature Publishing Group, pp. 168–169, 2010."},"date_published":"2010-03-01T00:00:00Z","date_updated":"2021-01-12T07:53:28Z","issue":"3","status":"public","fulldoi":"https://doi.org/10.1038/nmeth0310-168","doi":"10.1038/nmeth0310-168","title":"Lifeact mice for studying F-actin dynamics","month":"03","publication":"Nature Methods","page":"168 - 169","year":"2010","_id":"3957","publisher":"Nature Publishing Group","volume":7,"type":"journal_article","publication_status":"published","quality_controlled":0,"day":"01"}]