[{"intvolume":"        22","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","corr_author":"1","OA_type":"hybrid","date_published":"2026-01-05T00:00:00Z","ddc":["570"],"author":[{"orcid":"0000-0002-6425-5788","id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E","full_name":"Mishra, Nikhil","last_name":"Mishra","first_name":"Nikhil"},{"full_name":"Li, Yuting I","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","first_name":"Yuting I","last_name":"Li"},{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"year":"2026","PlanS_conform":"1","day":"05","file":[{"success":1,"file_name":"2026_NaturePhysics_Mishra.pdf","file_id":"21026","creator":"dernst","content_type":"application/pdf","date_updated":"2026-01-21T08:21:11Z","file_size":7335694,"checksum":"0ab7ac2fbcb61a364dba57152db64ed7","date_created":"2026-01-21T08:21:11Z","relation":"main_file","access_level":"open_access"}],"publication_status":"published","status":"public","date_updated":"2026-04-28T12:55:30Z","article_type":"original","publisher":"Springer Nature","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"external_id":{"oaworkid":["W7118187193"]},"page":"139-150","article_processing_charge":"Yes (via OA deal)","oaworkid":1,"project":[{"name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411"},{"_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413","name":"IST-BRIDGE: International postdoctoral program","call_identifier":"H2020"},{"_id":"917c023a-16d5-11f0-9cad-eb5cafc52090","name":"Cytoplasmic self-organization into cell-like compartments as a common guiding principle in early animal development"}],"ec_funded":1,"volume":22,"abstract":[{"text":"Early embryo geometry is one of the most invariant species-specific traits, yet its role in ensuring developmental reproducibility and robustness remains underexplored. Here we show that in zebrafish, the geometry of the fertilized egg—specifically its curvature and volume—serves as a critical initial condition triggering a cascade of events that influence development. The embryo geometry guides patterned asymmetric cell divisions in the blastoderm, generating radial gradients of cell volume and nucleocytoplasmic ratio. These gradients generate mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell cycle periods independently of other cells. We demonstrate that reducing cell autonomy reshapes these waves, emphasizing the instructive role of geometry-derived volume patterns in setting the intrinsic period of the cell cycle oscillator. In addition to organizing cell cycles, early embryo geometry spatially patterns zygotic genome activation at the midblastula transition, a key step in establishing embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation pattern and causes ectopic germ layer specification, underscoring the developmental significance of geometry. Together, our findings reveal a symmetry-breaking function of early embryo geometry in coordinating cell cycle and transcriptional patterning.","lang":"eng"}],"publication":"Nature Physics","file_date_updated":"2026-01-21T08:21:11Z","title":"Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo","OA_place":"publisher","language":[{"iso":"eng"}],"quality_controlled":"1","oa_version":"Published Version","oa":1,"department":[{"_id":"EdHa"},{"_id":"CaHe"}],"acknowledgement":"We thank N. Petridou (EMBL) for sharing results before publication. N.M. was supported by funding from the European Union’s Horizon 2020 programme under the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project ID 1.844. We would like to thank past and present members of the Heisenberg and Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic, N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria through resources provided by the Aquatics Facility, Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab Support Facility (LSF). Open access funding provided by Institute of Science and Technology (IST Austria).","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"],"issnl":[" 1745-2473"]},"_id":"21015","related_material":{"link":[{"description":"News on ISTA website","relation":"research_data","url":"https://ista.ac.at/en/news/geometry-shapes-life/"}]},"date_created":"2026-01-20T10:12:19Z","doi":"10.1038/s41567-025-03122-1","type":"journal_article","month":"01","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","citation":{"ama":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. 2026;22:139-150. doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>","chicago":"Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>.","short":"N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026) 139–150.","mla":"Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>, vol. 22, Springer Nature, 2026, pp. 139–50, doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>.","ieee":"N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo,” <i>Nature Physics</i>, vol. 22. Springer Nature, pp. 139–150, 2026.","apa":"Mishra, N., Li, Y. I., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2026). Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>","ista":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. 22, 139–150."},"scopus_import":"1"},{"department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}],"oa_version":"Preprint","oa":1,"language":[{"iso":"eng"}],"OA_place":"repository","citation":{"short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, (n.d.).","mla":"Dunajova, Zuzana, et al. <i>Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening</i>. bioRxiv, doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening.” bioRxiv.","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” bioRxiv, n.d. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>.","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., &#38; Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>"},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","month":"09","type":"preprint","date_created":"2026-03-11T08:40:06Z","doi":"10.1101/2025.05.20.655037","_id":"21427","related_material":{"record":[{"id":"21423","status":"public","relation":"dissertation_contains"},{"id":"21439","status":"public","relation":"research_data"}]},"acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.05.20.655037"}],"project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793"},{"name":"Motile active matter models of migrating cells and chiral filaments","_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","grant_number":"26360"}],"article_processing_charge":"No","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","abstract":[{"lang":"eng","text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo."}],"day":"25","year":"2025","publisher":"bioRxiv","date_updated":"2026-06-10T09:41:11Z","status":"public","publication_status":"draft","has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"author":[{"id":"4B39F286-F248-11E8-B48F-1D18A9856A87","full_name":"Dunajova, Zuzana","last_name":"Dunajova","first_name":"Zuzana"},{"first_name":"Saren","last_name":"Tasciyan","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Majek, Juraj","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","first_name":"Juraj","last_name":"Majek"},{"last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"last_name":"Sahai","first_name":"Erik","full_name":"Sahai, Erik"},{"last_name":"Sixt","first_name":"Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"},{"first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"}],"ddc":["539","570"],"date_published":"2025-09-25T00:00:00Z","corr_author":"1"},{"author":[{"last_name":"Brückner","first_name":"David","orcid":"0000-0001-7205-2975","id":"e1e86031-6537-11eb-953a-f7ab92be508d","full_name":"Brückner, David"},{"orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","last_name":"Hannezo","first_name":"Edouard B"}],"corr_author":"1","OA_type":"closed access","date_published":"2025-04-01T00:00:00Z","intvolume":"        17","isi":1,"date_updated":"2025-12-30T07:08:34Z","publisher":"Cold Spring Harbor Laboratory Press","article_type":"original","status":"public","publication_status":"published","pmid":1,"year":"2025","day":"01","article_number":"a041653","title":"Tissue active matter: Integrating mechanics and signaling into dynamical models","publication":"Cold Spring Harbor Perspectives in Biology","abstract":[{"lang":"eng","text":"The importance of physical forces in the morphogenesis, homeostatic function, and pathological dysfunction of multicellular tissues is being increasingly characterized, both theoretically and experimentally. Analogies between biological systems and inert materials such as foams, gels, and liquid crystals have provided striking insights into the core design principles underlying multicellular organization. However, these connections can seem surprising given that a key feature of multicellular systems is their ability to constantly consume energy, providing an active origin for the forces that they produce. Key emerging questions are, therefore, to understand whether and how this activity grants tissues novel properties that do not have counterparts in classical materials, as well as their consequences for biological function. Here, we review recent discoveries at the intersection of active matter and tissue biology, with an emphasis on how modeling and experiments can be combined to understand the dynamics of multicellular systems. These approaches suggest that a number of key biological tissue-scale phenomena, such as morphogenetic shape changes, collective migration, or fate decisions, share unifying design principles that can be described by physical models of tissue active matter."}],"volume":17,"ec_funded":1,"article_processing_charge":"No","external_id":{"isi":["001456660400001"],"pmid":["38951023"]},"project":[{"grant_number":"ALTF 343-2022","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","name":"A mechano-chemical theory for stem cell fate decisions in organoid development"},{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis"}],"month":"04","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","scopus_import":"1","citation":{"short":"D. Brückner, E.B. Hannezo, Cold Spring Harbor Perspectives in Biology 17 (2025).","mla":"Brückner, David, and Edouard B. Hannezo. “Tissue Active Matter: Integrating Mechanics and Signaling into Dynamical Models.” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 17, no. 4, a041653, Cold Spring Harbor Laboratory Press, 2025, doi:<a href=\"https://doi.org/10.1101/cshperspect.a041653\">10.1101/cshperspect.a041653</a>.","ieee":"D. Brückner and E. B. Hannezo, “Tissue active matter: Integrating mechanics and signaling into dynamical models,” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 17, no. 4. Cold Spring Harbor Laboratory Press, 2025.","ama":"Brückner D, Hannezo EB. Tissue active matter: Integrating mechanics and signaling into dynamical models. <i>Cold Spring Harbor Perspectives in Biology</i>. 2025;17(4). doi:<a href=\"https://doi.org/10.1101/cshperspect.a041653\">10.1101/cshperspect.a041653</a>","chicago":"Brückner, David, and Edouard B Hannezo. “Tissue Active Matter: Integrating Mechanics and Signaling into Dynamical Models.” <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press, 2025. <a href=\"https://doi.org/10.1101/cshperspect.a041653\">https://doi.org/10.1101/cshperspect.a041653</a>.","ista":"Brückner D, Hannezo EB. 2025. Tissue active matter: Integrating mechanics and signaling into dynamical models. Cold Spring Harbor Perspectives in Biology. 17(4), a041653.","apa":"Brückner, D., &#38; Hannezo, E. B. (2025). Tissue active matter: Integrating mechanics and signaling into dynamical models. <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press. <a href=\"https://doi.org/10.1101/cshperspect.a041653\">https://doi.org/10.1101/cshperspect.a041653</a>"},"acknowledgement":"We thank Fridtjof Brauns, Anna Kicheva, and Carl-Philipp Heisenberg for a critical reading of the manuscript and Claudia Flandoli for the artwork in the figures. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). This work received funding from the European Research Council (ERC) under the European Union\\u2019s Horizon 2020 Research and Innovation Programme Grant Agreement no. 851288.","doi":"10.1101/cshperspect.a041653","date_created":"2025-01-29T13:33:47Z","publication_identifier":{"issn":["1943-0264"]},"_id":"18960","oa_version":"None","department":[{"_id":"EdHa"}],"issue":"4","quality_controlled":"1","language":[{"iso":"eng"}]},{"OA_type":"hybrid","corr_author":"1","date_published":"2025-02-28T00:00:00Z","ddc":["530"],"author":[{"last_name":"Xue","first_name":"Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","full_name":"Xue, Shi-lei"},{"first_name":"Qiutan","last_name":"Yang","full_name":"Yang, Qiutan"},{"first_name":"Prisca","last_name":"Liberali","full_name":"Liberali, Prisca"},{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo"}],"intvolume":"        21","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"has_accepted_license":"1","pmid":1,"publication_status":"published","status":"public","date_updated":"2025-09-30T10:47:36Z","publisher":"Springer Nature","article_type":"original","year":"2025","PlanS_conform":"1","day":"28","file":[{"success":1,"file_id":"20129","file_name":"2025_NaturePhysics_Xue.pdf","file_size":16302436,"content_type":"application/pdf","date_updated":"2025-08-05T12:12:03Z","creator":"dernst","access_level":"open_access","relation":"main_file","date_created":"2025-08-05T12:12:03Z","checksum":"fb5e59be145b95f9851d3d7c9dbb85e6"}],"ec_funded":1,"volume":21,"publication":"Nature Physics","abstract":[{"lang":"eng","text":"Reproducible pattern and form generation during embryogenesis is poorly understood. Intestinal organoid morphogenesis involves a number of mechanochemical regulators such as cell-type-specific cytoskeletal forces and osmotically driven lumen volume changes. It is unclear how these forces are coordinated in time and space to ensure robust morphogenesis. Here we show how mechanosensitive feedback on cytoskeletal tension gives rise to morphological bistability in a minimal model of organoid morphogenesis. In the model, lumen volume changes can impact the epithelial shape via both direct mechanical and indirect mechanosensitive mechanisms. We find that both bulged and budded crypt states are possible and dependent on the history of volume changes. We test key modelling assumptions via biophysical and pharmacological experiments to demonstrate how bistability can explain experimental observations, such as the importance of the timing of lumen shrinkage and robustness of the final morphogenetic state to mechanical perturbations. This suggests that bistability arising from feedback between cellular tensions and fluid pressure could be a general mechanism that coordinates multicellular shape changes in developing systems."}],"file_date_updated":"2025-08-05T12:12:03Z","title":"Mechanochemical bistability of intestinal organoids enables robust morphogenesis","article_number":"078104","arxiv":1,"external_id":{"isi":["001434072800001"],"pmid":["40248571"],"arxiv":["2403.19900"]},"article_processing_charge":"Yes (via OA deal)","project":[{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020"},{"grant_number":"P31639","_id":"268294B6-B435-11E9-9278-68D0E5697425","name":"Active mechano-chemical description of the cell cytoskeleton","call_identifier":"FWF"}],"acknowledgement":"We thank all members of the Hannezo and Liberali groups for fruitful discussions, as well as C. Schwayer, G. Quintas, L. Capolupo, D. Bruckner and D. Pinheiro for reading the manuscript. We also thank Y. Wu and X. Wu from the Yang group for performing experiments in the last rounds of revision and the So group at the National Institute of Biological Sciences, Beijing, for helping with the light-sheet time-lapse experiments. This work received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme via grant agreement no. 758617 (to P.L.), Swiss National Foundation (SNF) (no. POOP3_157531 to P.L.), the ERC under the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 851288 (to E.H.) and the Austrian Science Fund (FWF) (no. P 31639 to E.H.). This work was supported by the National Natural Science Foundation of China via grant no.3247060387 (to Q.Y.) and the Strategic Priority Research Program of the Chinese Academy of Sciences (no. XDB0820000 to Q.Y.) . Open access funding provided by Institute of Science and Technology (IST Austria).","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"_id":"19373","doi":"10.1038/s41567-025-02792-1","date_created":"2025-03-09T23:01:28Z","type":"journal_article","month":"02","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","citation":{"short":"S. Xue, Q. Yang, P. Liberali, E.B. Hannezo, Nature Physics 21 (2025).","mla":"Xue, Shi-lei, et al. “Mechanochemical Bistability of Intestinal Organoids Enables Robust Morphogenesis.” <i>Nature Physics</i>, vol. 21, 078104, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41567-025-02792-1\">10.1038/s41567-025-02792-1</a>.","ieee":"S. Xue, Q. Yang, P. Liberali, and E. B. Hannezo, “Mechanochemical bistability of intestinal organoids enables robust morphogenesis,” <i>Nature Physics</i>, vol. 21. Springer Nature, 2025.","ama":"Xue S, Yang Q, Liberali P, Hannezo EB. Mechanochemical bistability of intestinal organoids enables robust morphogenesis. <i>Nature Physics</i>. 2025;21. doi:<a href=\"https://doi.org/10.1038/s41567-025-02792-1\">10.1038/s41567-025-02792-1</a>","chicago":"Xue, Shi-lei, Qiutan Yang, Prisca Liberali, and Edouard B Hannezo. “Mechanochemical Bistability of Intestinal Organoids Enables Robust Morphogenesis.” <i>Nature Physics</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41567-025-02792-1\">https://doi.org/10.1038/s41567-025-02792-1</a>.","ista":"Xue S, Yang Q, Liberali P, Hannezo EB. 2025. Mechanochemical bistability of intestinal organoids enables robust morphogenesis. Nature Physics. 21, 078104.","apa":"Xue, S., Yang, Q., Liberali, P., &#38; Hannezo, E. B. (2025). Mechanochemical bistability of intestinal organoids enables robust morphogenesis. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-02792-1\">https://doi.org/10.1038/s41567-025-02792-1</a>"},"scopus_import":"1","OA_place":"publisher","language":[{"iso":"eng"}],"quality_controlled":"1","oa":1,"oa_version":"Published Version","department":[{"_id":"EdHa"}]},{"year":"2025","day":"01","file":[{"access_level":"open_access","date_created":"2025-12-29T14:13:01Z","relation":"main_file","checksum":"a2b313de3cacb53f20f2b91c42612ad9","content_type":"application/pdf","date_updated":"2025-12-29T14:13:01Z","file_size":7301679,"creator":"dernst","file_id":"20874","file_name":"2025_JourInvestigativeDerma_Andersen.pdf","success":1}],"status":"public","pmid":1,"publication_status":"published","date_updated":"2025-12-29T14:13:43Z","article_type":"original","publisher":"Elsevier","intvolume":"       145","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"isi":1,"has_accepted_license":"1","OA_type":"hybrid","corr_author":"1","date_published":"2025-09-01T00:00:00Z","ddc":["570"],"author":[{"last_name":"Andersen","first_name":"Marianne S.","full_name":"Andersen, Marianne S."},{"last_name":"Ulyanchenko","first_name":"Svetlana","full_name":"Ulyanchenko, Svetlana"},{"last_name":"Schweiger","first_name":"Pawel J.","full_name":"Schweiger, Pawel J."},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"},{"full_name":"Simons, Benjamin D.","first_name":"Benjamin D.","last_name":"Simons"},{"first_name":"Kim B.","last_name":"Jensen","full_name":"Jensen, Kim B."}],"OA_place":"publisher","issue":"9","quality_controlled":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","oa":1,"department":[{"_id":"EdHa"}],"acknowledgement":"We thank the members of the Jensen Laboratory for experimental and technical advice, the imaging facilities at reNEW, and animal caretakers for expert assistance. This work was supported by the Lundbeck Foundation (R105-A9755 to KBJ) and the Leo Pharma Foundation (LF-OC-20-000169). The Novo Nordisk Foundation Center for Stem Cell Medicine was supported by a Novo Nordisk Foundation grant (NNF21CC0073729). B.D.S. was supported by the Wellcome Trust (219478/Z/19/Z) and a Royal Society EP Abraham Research Professorship (RP/R1/180165 and RP\\R\\231004). Figure elements were adapted from Bio-Render. KBJ is the lead contact and guarantor of this study.","doi":"10.1016/j.jid.2025.01.034","date_created":"2025-04-06T22:01:32Z","_id":"19507","publication_identifier":{"issn":["0022-202X"],"eissn":["1523-1747"]},"month":"09","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","scopus_import":"1","citation":{"apa":"Andersen, M. S., Ulyanchenko, S., Schweiger, P. J., Hannezo, E. B., Simons, B. D., &#38; Jensen, K. B. (2025). Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. <i>Journal of Investigative Dermatology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">https://doi.org/10.1016/j.jid.2025.01.034</a>","ista":"Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB. 2025. Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. Journal of Investigative Dermatology. 145(9), 2191–2202.e5.","ama":"Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB. Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. <i>Journal of Investigative Dermatology</i>. 2025;145(9):2191-2202.e5. doi:<a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">10.1016/j.jid.2025.01.034</a>","chicago":"Andersen, Marianne S., Svetlana Ulyanchenko, Pawel J. Schweiger, Edouard B Hannezo, Benjamin D. Simons, and Kim B. Jensen. “Spatiotemporal Switches in Progenitor Cell Fate Govern Upper Hair Follicle Growth and Maintenance.” <i>Journal of Investigative Dermatology</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">https://doi.org/10.1016/j.jid.2025.01.034</a>.","short":"M.S. Andersen, S. Ulyanchenko, P.J. Schweiger, E.B. Hannezo, B.D. Simons, K.B. Jensen, Journal of Investigative Dermatology 145 (2025) 2191–2202.e5.","ieee":"M. S. Andersen, S. Ulyanchenko, P. J. Schweiger, E. B. Hannezo, B. D. Simons, and K. B. Jensen, “Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance,” <i>Journal of Investigative Dermatology</i>, vol. 145, no. 9. Elsevier, p. 2191–2202.e5, 2025.","mla":"Andersen, Marianne S., et al. “Spatiotemporal Switches in Progenitor Cell Fate Govern Upper Hair Follicle Growth and Maintenance.” <i>Journal of Investigative Dermatology</i>, vol. 145, no. 9, Elsevier, 2025, p. 2191–2202.e5, doi:<a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">10.1016/j.jid.2025.01.034</a>."},"article_processing_charge":"No","external_id":{"isi":["001604396400001"],"pmid":["40010488"]},"page":"2191-2202.e5","abstract":[{"text":"The epidermis provides a protective barrier against hostile environments. However, our knowledge of how this barrier forms during development and is subsequently maintained remains incomplete. The infundibulum is a cylindrical epidermal tissue compartment that serves as an outlet for hair follicles protruding from the skin and the excretion of the sebaceous glands that are essential for proper skin function. In this study, we applied quantitative fate mapping to address how infundibulum are maintained during adulthood. We demonstrate that progenitors build and maintain tissues through stochastic cell fate choices. Long-term analysis identified a preferential transient contribution from cells initially located at the bottom of the structure to the maintenance of the tissue, with bursts of local progenitor expansion associated with the phases of hair growth. Beyond providing compartment-wide insights into progenitor cell dynamics in infundibulum, these findings demonstrate how spatiotemporal regulation controls transient progenitor dominance.","lang":"eng"}],"publication":"Journal of Investigative Dermatology","volume":145,"file_date_updated":"2025-12-29T14:13:01Z","title":"Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance"},{"OA_place":"publisher","issue":"17","quality_controlled":"1","language":[{"iso":"eng"}],"oa_version":"Published Version","oa":1,"department":[{"_id":"EdHa"}],"acknowledgement":"We thank A. Dimitracopoulos, K. Kawaguchi, J. Vidigueira, B. Baum, I. McLaren, D. St Johnston, and members of the Buckley, Scarpa, Steventon, Kawaguchi, and Xiong labs for technical assistance and constructive feedback. We thank Ryan Greenhalgh for methods developed to obtain fluidity values from AFM data. We thank Nicola Lawrence, Alex Sossick, and Sargon Gross-Thebing from the Gurdon Institute Imaging Facility for microscopy support. Funding: this work was supported by a Wellcome Trust/Royal Society Sir Henry Dale Fellowship (215439/Z/19/Z) and UKRI-EPSRC Frontier Research Grant (EP/X023761/1, originally selected as an ERC Starting Grant) to F.X.; an ERC Consolidator Grant (772426), ERC Synergy Grant 101118729 UNFOLD, and Alexander von Humboldt Professorship ( Alexander von Humboldt Foundation) to K.F.; and an ERC Starting Grant (851288) to E.H.","doi":"10.1016/j.devcel.2025.04.010","date_created":"2025-05-18T22:02:50Z","_id":"19703","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"month":"09","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","scopus_import":"1","citation":{"short":"S.B.P. Mclaren, S. Xue, S. Ding, A.K. Winkel, O. Baldwin, S. Dwarakacherla, K. Franze, E.B. Hannezo, F. Xiong, Developmental Cell 60 (2025) 2237–2247.e4.","ieee":"S. B. P. Mclaren <i>et al.</i>, “Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord,” <i>Developmental Cell</i>, vol. 60, no. 17. Elsevier, p. 2237–2247.e4, 2025.","mla":"Mclaren, Susannah B. P., et al. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” <i>Developmental Cell</i>, vol. 60, no. 17, Elsevier, 2025, p. 2237–2247.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">10.1016/j.devcel.2025.04.010</a>.","ama":"Mclaren SBP, Xue S, Ding S, et al. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. <i>Developmental Cell</i>. 2025;60(17):2237-2247.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">10.1016/j.devcel.2025.04.010</a>","chicago":"Mclaren, Susannah B.P., Shi-lei Xue, Siyuan Ding, Alexander K. Winkel, Oscar Baldwin, Shreya Dwarakacherla, Kristian Franze, Edouard B Hannezo, and Fengzhu Xiong. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">https://doi.org/10.1016/j.devcel.2025.04.010</a>.","ista":"Mclaren SBP, Xue S, Ding S, Winkel AK, Baldwin O, Dwarakacherla S, Franze K, Hannezo EB, Xiong F. 2025. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. Developmental Cell. 60(17), 2237–2247.e4.","apa":"Mclaren, S. B. P., Xue, S., Ding, S., Winkel, A. K., Baldwin, O., Dwarakacherla, S., … Xiong, F. (2025). Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">https://doi.org/10.1016/j.devcel.2025.04.010</a>"},"article_processing_charge":"Yes (in subscription journal)","page":"2237-2247.e4","external_id":{"isi":["001570502100005"],"pmid":["40347948"]},"project":[{"grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020"}],"abstract":[{"lang":"eng","text":"An enlarged brain underlies the complex central nervous system of vertebrates. The dramatic expansion of the brain that diverges its shape from the spinal cord follows neural tube closure during embryonic development. Here, we show that this differential deformation is encoded by a pre-pattern of tissue material properties in chicken embryos. Using magnetic droplets and atomic force microscopy, we demonstrate that the dorsal hindbrain is more fluid than the dorsal spinal cord, resulting in a thinning versus a resisting response to increasing lumen pressure, respectively. The dorsal hindbrain exhibits reduced apical actin and a disorganized laminin matrix consistent with tissue fluidization. Blocking the activity of neural-crest-associated matrix metalloproteinases inhibits hindbrain expansion. Transplanting dorsal hindbrain cells to the spinal cord can locally create an expanded brain-like morphology in some cases. Our findings raise questions in vertebrate head evolution and suggest a general role of mechanical pre-patterning in sculpting epithelial tubes."}],"publication":"Developmental Cell","volume":60,"ec_funded":1,"file_date_updated":"2025-12-29T13:45:05Z","title":"Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord","year":"2025","day":"08","PlanS_conform":"1","file":[{"access_level":"open_access","date_created":"2025-12-29T13:45:05Z","relation":"main_file","checksum":"1ca6f0822c1cbd430686d5e2a4f96401","date_updated":"2025-12-29T13:45:05Z","content_type":"application/pdf","file_size":12564806,"creator":"dernst","file_id":"20872","file_name":"2025_DevelopmentalCell_McLaren.pdf","success":1}],"status":"public","pmid":1,"publication_status":"published","date_updated":"2025-12-29T14:58:14Z","publisher":"Elsevier","article_type":"original","intvolume":"        60","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","OA_type":"hybrid","date_published":"2025-09-08T00:00:00Z","ddc":["570"],"author":[{"first_name":"Susannah B.P.","last_name":"Mclaren","full_name":"Mclaren, Susannah B.P."},{"last_name":"Xue","first_name":"Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","full_name":"Xue, Shi-lei"},{"full_name":"Ding, Siyuan","first_name":"Siyuan","last_name":"Ding"},{"last_name":"Winkel","first_name":"Alexander K.","full_name":"Winkel, Alexander K."},{"full_name":"Baldwin, Oscar","last_name":"Baldwin","first_name":"Oscar"},{"full_name":"Dwarakacherla, Shreya","first_name":"Shreya","last_name":"Dwarakacherla"},{"first_name":"Kristian","last_name":"Franze","full_name":"Franze, Kristian"},{"first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"},{"full_name":"Xiong, Fengzhu","first_name":"Fengzhu","last_name":"Xiong"}]},{"file_date_updated":"2025-07-31T07:28:54Z","abstract":[{"lang":"eng","text":"Introduction: Acid-growth theory has been postulated in the 70s to explain the rapid elongation of plant cells in response to the hormone auxin. More recently, it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting acidification of the apoplast is the principal mechanism by which auxin and other hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances, the impact of this acidification on the mechanical properties of the cell wall remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate hormone-induced tissue elongation and local changes in cell wall mechanical properties. Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase (PME), along with calcium chelators, we investigate the effect of pectin modification in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification of apoplast is necessary and sufficient to induce cell elongation through promoting cell wall softening. Moreover, we show that enhanced PME activity can induce both cell wall softening or stiffening in extracellular calcium dependent-manner and that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion: Our results confirm a dual role of PME in plant cell elongation. However, further investigation is needed to assess the status of pectin following short- or long-term PME treatments in order to determine if pectin methyl-esterification might promote its degradation as well as the role of PME inhibitors upon PME induction."}],"publication":"Frontiers in Plant Science","ec_funded":1,"volume":16,"article_number":"1612366","title":"Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"}],"project":[{"name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362"},{"_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854","name":"IST Austria Open Access Fund"}],"article_processing_charge":"Yes","external_id":{"pmid":["40688689"],"isi":["001530690900001"]},"date_created":"2025-07-27T22:01:26Z","doi":"10.3389/fpls.2025.1612366","publication_identifier":{"eissn":["1664-462X"]},"_id":"20080","acknowledgement":"The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127). We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler for the efforts to establish immunodetection method.","scopus_import":"1","citation":{"short":"M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B. Hannezo, E. Benková, Frontiers in Plant Science 16 (2025).","ieee":"M. Gallemi <i>et al.</i>, “Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties,” <i>Frontiers in Plant Science</i>, vol. 16. Frontiers Media, 2025.","mla":"Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>, vol. 16, 1612366, Frontiers Media, 2025, doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>.","ama":"Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. 2025;16. doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>","chicago":"Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl, Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2025. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>.","ista":"Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB, Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.","apa":"Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P., Hannezo, E. B., &#38; Benková, E. (2025). Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>"},"month":"07","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","language":[{"iso":"eng"}],"quality_controlled":"1","OA_place":"publisher","DOAJ_listed":"1","department":[{"_id":"EdHa"},{"_id":"EvBe"},{"_id":"CaGu"}],"oa":1,"oa_version":"Published Version","date_published":"2025-07-04T00:00:00Z","APC_amount":"3642,79 EUR","ddc":["580"],"OA_type":"gold","corr_author":"1","author":[{"first_name":"Marçal","last_name":"Gallemi","orcid":"0000-0003-4675-6893","full_name":"Gallemi, Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Montesinos López","first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099"},{"first_name":"Nikola","last_name":"Zarevski","full_name":"Zarevski, Nikola","id":"18e95355-e05a-11ea-a9c0-8fba1b89e83a"},{"full_name":"Pribyl, Jan","last_name":"Pribyl","first_name":"Jan"},{"last_name":"Skládal","first_name":"Petr","full_name":"Skládal, Petr"},{"first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","first_name":"Eva"}],"isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        16","has_accepted_license":"1","status":"public","publication_status":"published","pmid":1,"publisher":"Frontiers Media","article_type":"original","date_updated":"2026-05-20T07:53:03Z","day":"04","PlanS_conform":"1","year":"2025","file":[{"file_name":"2025_FrontiersPlantSc_Gallemi.pdf","file_id":"20093","success":1,"relation":"main_file","checksum":"9e6b8b53ba56d4a24a9bd91cf6d2dc58","date_created":"2025-07-31T07:28:54Z","access_level":"open_access","creator":"dernst","date_updated":"2025-07-31T07:28:54Z","content_type":"application/pdf","file_size":3665187}]},{"article_number":"e2504064122","title":"Self-generated chemotaxis of mixed cell populations","file_date_updated":"2025-09-08T07:23:29Z","volume":122,"ec_funded":1,"publication":"Proceedings of the National Academy of Sciences","abstract":[{"lang":"eng","text":"Cell and tissue movement in development, cancer invasion, and immune response relies on chemical or mechanical guidance cues. In many systems, this behavior is locally directed by self-generated signaling gradients rather than long-range, prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally and navigate through self-generated gradients remains largely unexplored. Here, we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous cell populations. We find that the relative chemotactic sensitivities of different cell populations control their long-time coupling and comigration dynamics, with boundary conditions such as external cell and attractant reservoirs substantially influencing the migration patterns. Our model predicts an optimal parameter regime that enables robust and colocalized migration. We test our theoretical predictions with in vitro experiments demonstrating the comigration of distinct immune cell populations, and quantitatively reproduce observed migration patterns under wild-type and perturbed conditions. Interestingly, immune cell comigration occurs close to the predicted optimal regime. Finally, we incorporate mechanical interactions into our framework, revealing a nontrivial interplay between chemotactic and mechanical nonreciprocity in driving collective migration. Together, our findings suggest that self-generated chemotaxis is a robust strategy for the navigation of mixed cell populations."}],"project":[{"grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis"}],"article_processing_charge":"Yes (in subscription journal)","external_id":{"pmid":["40838890"],"isi":["001562181600001"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"NanoFab"}],"citation":{"chicago":"Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>.","ama":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(34). doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>","mla":"Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34, e2504064122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>.","ieee":"M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated chemotaxis of mixed cell populations,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34. National Academy of Sciences, 2025.","short":"M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of the National Academy of Sciences 122 (2025).","apa":"Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., &#38; Hannezo, E. B. (2025). Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>","ista":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34), e2504064122."},"scopus_import":"1","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"08","_id":"20289","publication_identifier":{"issn":["0027-8424"],"eissn":["1091-6490"]},"related_material":{"link":[{"relation":"software","url":"https://github.com/mehmetcanucar/Self-generated-chemotaxis"}]},"doi":"10.1073/pnas.2504064122","date_created":"2025-09-07T22:01:32Z","acknowledgement":"We thank all members of the M.S. and E.H. groups for stimulating discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support facility of the Institute of Science and Technology Austria for their excellent support and provided resources for the experimental research. In particular, we thank Jack Merrin from the Nanofabrication facility who generated the microfabricated channel used in this study. This work received funding fromt he European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield Strategic Research Fellowship in the Physics of Life and Quantitative Biology.","department":[{"_id":"EdHa"},{"_id":"MiSi"}],"oa":1,"oa_version":"Published Version","language":[{"iso":"eng"}],"quality_controlled":"1","issue":"34","OA_place":"publisher","author":[{"first_name":"Mehmet C","last_name":"Ucar","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C","id":"50B2A802-6007-11E9-A42B-EB23E6697425"},{"orcid":"0009-0003-0415-7603","id":"60f7509a-f652-11ea-9d86-b963d6490d7c","full_name":"Zane, Alsberga","last_name":"Zane","first_name":"Alsberga"},{"orcid":"0000-0002-7698-3061","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","full_name":"Alanko, Jonna H","last_name":"Alanko","first_name":"Jonna H"},{"full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt"},{"last_name":"Hannezo","first_name":"Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"}],"APC_amount":"5766,07 EUR","ddc":["570"],"date_published":"2025-08-26T00:00:00Z","OA_type":"hybrid","corr_author":"1","has_accepted_license":"1","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"       122","article_type":"original","publisher":"National Academy of Sciences","date_updated":"2026-05-20T08:59:54Z","pmid":1,"publication_status":"published","status":"public","file":[{"file_name":"2025_PNAS_Ucar.pdf","file_id":"20307","success":1,"date_created":"2025-09-08T07:23:29Z","checksum":"b36abd92673b6d76376fc9434bad52cc","relation":"main_file","access_level":"open_access","creator":"dernst","date_updated":"2025-09-08T07:23:29Z","content_type":"application/pdf","file_size":16069140}],"PlanS_conform":"1","day":"26","year":"2025"},{"department":[{"_id":"EdHa"}],"DOAJ_listed":"1","oa":1,"oa_version":"Published Version","quality_controlled":"1","language":[{"iso":"eng"}],"OA_place":"publisher","scopus_import":"1","citation":{"ieee":"P. Sahu, S. Monteiro-Ferreira, S. Canato, R. M. Soares, A. Sánchez-Danés, and E. B. Hannezo, “Mechanical control of cell fate decisions in the skin epidermis,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","mla":"Sahu, Preeti, et al. “Mechanical Control of Cell Fate Decisions in the Skin Epidermis.” <i>Nature Communications</i>, vol. 16, 8440, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-62882-9\">10.1038/s41467-025-62882-9</a>.","short":"P. Sahu, S. Monteiro-Ferreira, S. Canato, R.M. Soares, A. Sánchez-Danés, E.B. Hannezo, Nature Communications 16 (2025).","chicago":"Sahu, Preeti, Sara Monteiro-Ferreira, Sara Canato, Raquel Maia Soares, Adriana Sánchez-Danés, and Edouard B Hannezo. “Mechanical Control of Cell Fate Decisions in the Skin Epidermis.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-62882-9\">https://doi.org/10.1038/s41467-025-62882-9</a>.","ama":"Sahu P, Monteiro-Ferreira S, Canato S, Soares RM, Sánchez-Danés A, Hannezo EB. Mechanical control of cell fate decisions in the skin epidermis. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-62882-9\">10.1038/s41467-025-62882-9</a>","ista":"Sahu P, Monteiro-Ferreira S, Canato S, Soares RM, Sánchez-Danés A, Hannezo EB. 2025. Mechanical control of cell fate decisions in the skin epidermis. Nature Communications. 16, 8440.","apa":"Sahu, P., Monteiro-Ferreira, S., Canato, S., Soares, R. M., Sánchez-Danés, A., &#38; Hannezo, E. B. (2025). Mechanical control of cell fate decisions in the skin epidermis. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-62882-9\">https://doi.org/10.1038/s41467-025-62882-9</a>"},"month":"09","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","date_created":"2025-10-05T22:01:34Z","doi":"10.1038/s41467-025-62882-9","publication_identifier":{"eissn":["2041-1723"]},"_id":"20424","acknowledgement":"We thank Alois Schlögl, Paula Sanematsu, Susana Moreno Flores, Bernat Corominas-Murtra, Stefania Tavano, Gayathri Singharaju, and Hannezo group members for helpful discussions, the Bioimaging facility at ISTA, as well as Matthias Merkel and Lisa Manning for sharing the 3D Voronoi code. We also thank the Champalimaud animal facility, Anna Pezzarossa and the Champalimaud ABBE platform for the help with microscopy and image processing. This work was supported by EMBO (ALTF 522-2021), a Fundação para a Ciência e Tecnologia grant to A.S.D. (PTDC/MED-ONC/5553/2020), as well as the European Research Council (grant 851288 to EH). A.S.D., S.C., and R.M.S. are supported by QuantOCancer Project Horizon European Union’s Horizon 2020 program (grant agreement No 810653).","project":[{"_id":"628f3fb1-2b32-11ec-9570-83ce778803f7","grant_number":"ALTF 522-2021","name":"Biomechanics of stem cell fate determination"},{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020"}],"article_processing_charge":"Yes","external_id":{"isi":["001582555200011"],"pmid":["41006218"]},"acknowledged_ssus":[{"_id":"Bio"}],"title":"Mechanical control of cell fate decisions in the skin epidermis","article_number":"8440","file_date_updated":"2025-10-13T12:37:04Z","abstract":[{"lang":"eng","text":"Homeostasis relies on a precise balance of fate choices between renewal and differentiation. Although progress has been done to characterize the dynamics of single-cell fate choices, their underlying mechanistic basis often remains unclear. Concentrating on skin epidermis as a paradigm for multilayered tissues with complex fate choices, we develop a 3D vertex-based model with proliferation in the basal layer, showing that mechanical competition for space naturally gives rise to homeostasis and neutral drift dynamics that are seen experimentally. We then explore the effect of introducing mechanical heterogeneities between cellular subpopulations. We uncover that relatively small tension heterogeneities, reflected by distinct morphological changes in single-cell shapes, can be sufficient to heavily tilt cellular dynamics towards exponential growth. We thus derive a master relationship between cell shape and long-term clonal dynamics, which we validated during basal cell carcinoma initiation in mouse epidermis. Altogether, we propose a theoretical framework to link mechanical forces, quantitative cellular morphologies and cellular fate outcomes in complex tissues."}],"publication":"Nature Communications","volume":16,"ec_funded":1,"file":[{"date_created":"2025-10-13T12:37:04Z","checksum":"d1656576883b23902545328e2d640234","relation":"main_file","access_level":"open_access","creator":"dernst","file_size":2816813,"date_updated":"2025-10-13T12:37:04Z","content_type":"application/pdf","file_name":"2025_NatureComm_Sahu.pdf","file_id":"20464","success":1}],"day":"26","year":"2025","publisher":"Springer Nature","article_type":"original","date_updated":"2026-05-20T08:52:01Z","status":"public","pmid":1,"publication_status":"published","has_accepted_license":"1","isi":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"intvolume":"        16","author":[{"last_name":"Sahu","first_name":"Preeti","id":"55BA52EE-A185-11EA-88FD-18AD3DDC885E","full_name":"Sahu, Preeti"},{"full_name":"Monteiro-Ferreira, Sara","first_name":"Sara","last_name":"Monteiro-Ferreira"},{"full_name":"Canato, Sara","last_name":"Canato","first_name":"Sara"},{"first_name":"Raquel Maia","last_name":"Soares","full_name":"Soares, Raquel Maia"},{"first_name":"Adriana","last_name":"Sánchez-Danés","full_name":"Sánchez-Danés, Adriana"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"}],"APC_amount":"7068 EUR","ddc":["570"],"date_published":"2025-09-26T00:00:00Z","corr_author":"1","OA_type":"gold"},{"date_published":"2025-10-01T00:00:00Z","corr_author":"1","OA_type":"green","author":[{"last_name":"Fortunato","first_name":"Isabela Corina","full_name":"Fortunato, Isabela Corina"},{"full_name":"Brückner, David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","orcid":"0000-0001-7205-2975","first_name":"David","last_name":"Brückner"},{"first_name":"Steffen","last_name":"Grosser","full_name":"Grosser, Steffen"},{"first_name":"Rohit","last_name":"Nautiyal","full_name":"Nautiyal, Rohit"},{"full_name":"Rossetti, Leone","last_name":"Rossetti","first_name":"Leone"},{"last_name":"Bosch-Padrós","first_name":"Miquel","full_name":"Bosch-Padrós, Miquel"},{"first_name":"Jonel","last_name":"Trebicka","full_name":"Trebicka, Jonel"},{"first_name":"Pere","last_name":"Roca-Cusachs","full_name":"Roca-Cusachs, Pere"},{"full_name":"Sunyer, Raimon","last_name":"Sunyer","first_name":"Raimon"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"},{"first_name":"Xavier","last_name":"Trepat","full_name":"Trepat, Xavier"}],"isi":1,"intvolume":"        21","status":"public","publication_status":"published","publisher":"Springer Nature","article_type":"original","date_updated":"2026-01-05T14:26:28Z","day":"01","year":"2025","publication":"Nature Physics","abstract":[{"text":"Haptotaxis is the process of directed cell migration along gradients of extracellular matrix density and is central to morphogenesis, immune responses and cancer invasion. It is commonly assumed that cells respond to these gradients by migrating directionally towards the regions of highest ligand density. In contrast with this view, here we show that cells exposed to micropatterned fibronectin gradients exhibit a wide range of complex trajectories, including directed haptotactic migration up the gradient but also linear oscillations and circles with extended periods of migration down the gradient. To explain this behaviour, we developed a biophysical model of haptotactic cell migration based on a coarse-grained molecular clutch model coupled to persistent stochastic polarity dynamics. Although initial haptotactic migration is explained by the differential friction at the front and back of the cell, the observed complex trajectories over longer timescales arise from the interplay between differential friction, persistence and physical confinement. Overall, our study reveals that confinement and persistence modulate the ability of cells to sense and respond to haptotactic cues and provides a framework for understanding how cells navigate complex environments.","lang":"eng"}],"volume":21,"title":"Single-cell migration along and against confined haptotactic gradients","project":[{"name":"A mechano-chemical theory for stem cell fate decisions in organoid development","grant_number":"ALTF 343-2022","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"}],"article_processing_charge":"No","external_id":{"isi":["001581659900001"]},"page":"1638-1647","date_created":"2025-10-05T22:01:36Z","doi":"10.1038/s41567-025-03015-3","_id":"20431","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"acknowledgement":"We thank all the members of our groups for discussions and support. We thank A. Menéndez, S. Usieto, M. Purciolas and E. Coderch for technical assistance. We thank G. Charras (London Centre for Nanotechnology, UK) and M. Sheetz (Columbia University, USA) for sharing cells used in this work. We thank J. Ivaska (University of Turku, Finland) for sharing integrin α5-GFP DNA plasmid. We thank P. Guillamat for technical advice and A. Labernardie for providing the microfluidic channels. We thank M. Gómez-González for sharing the 2D traction microscopy algorithm. Finally, we thank P. Guillamat, J. Abenza, G. Ceada, L. Faure, E. Dalaka, M. Matejčić, A. Beedle, I. Granero, O. Baguer, A. Albajar and N. Chahare for discussions. This paper was funded by the Generalitat de Catalunya (Grant Nos. AGAUR SGR-2017-01602 to X.T. and 2021 SGR 00523 to R.S. and the CERCA Programme and ICREA Academia awards to P.R.-C.), the Spanish Ministry for Science and Innovation MICCINN/FEDER (Grant Nos. PID2021-128635NB-I00, MCIN/AEI/10.13039/501100011033 and ERDF-EU A way of making Europe to X.T., PID2021-128674OB-I00 and CNS2022-135533 to R.S. and PID2019-110298GB-I00 to P.R.-C.), the European Research Council (Grant Nos. 101097753 to P.R.-C. and Adv-883739 to X.T.), Fundació la Marató de TV3 (Project Award 201903-30-31-32 to X.T.), the European Commission (Grant No. H2020-FETPROACT-01-2016-731957 to P.R.-C. and X.T.) and La Caixa Foundation (Grant No. LCF/PR/HR20/52400004 to P.R.-C. and X.T.). R.S. is a Serra-Hunter fellow. D.B.B. was supported by the NOMIS foundation as a NOMIS fellow, by the European Molecular Biology Organization (Postdoctoral Fellowship ALTF 343-2022) and by the Austrian Academy of Sciences through an APART-MINT Fellowship. I.C.F. acknowledges support from the European Foundation for the Study of Chronic Liver Failure. IBEC is recipient of a Severo Ochoa Award of Excellence from MINECO.","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.12.02.626413"}],"scopus_import":"1","citation":{"chicago":"Fortunato, Isabela Corina, David Brückner, Steffen Grosser, Rohit Nautiyal, Leone Rossetti, Miquel Bosch-Padrós, Jonel Trebicka, et al. “Single-Cell Migration along and against Confined Haptotactic Gradients.” <i>Nature Physics</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41567-025-03015-3\">https://doi.org/10.1038/s41567-025-03015-3</a>.","ama":"Fortunato IC, Brückner D, Grosser S, et al. Single-cell migration along and against confined haptotactic gradients. <i>Nature Physics</i>. 2025;21:1638-1647. doi:<a href=\"https://doi.org/10.1038/s41567-025-03015-3\">10.1038/s41567-025-03015-3</a>","mla":"Fortunato, Isabela Corina, et al. “Single-Cell Migration along and against Confined Haptotactic Gradients.” <i>Nature Physics</i>, vol. 21, Springer Nature, 2025, pp. 1638–47, doi:<a href=\"https://doi.org/10.1038/s41567-025-03015-3\">10.1038/s41567-025-03015-3</a>.","ieee":"I. C. Fortunato <i>et al.</i>, “Single-cell migration along and against confined haptotactic gradients,” <i>Nature Physics</i>, vol. 21. Springer Nature, pp. 1638–1647, 2025.","short":"I.C. Fortunato, D. Brückner, S. Grosser, R. Nautiyal, L. Rossetti, M. Bosch-Padrós, J. Trebicka, P. Roca-Cusachs, R. Sunyer, E.B. Hannezo, X. Trepat, Nature Physics 21 (2025) 1638–1647.","apa":"Fortunato, I. C., Brückner, D., Grosser, S., Nautiyal, R., Rossetti, L., Bosch-Padrós, M., … Trepat, X. (2025). Single-cell migration along and against confined haptotactic gradients. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03015-3\">https://doi.org/10.1038/s41567-025-03015-3</a>","ista":"Fortunato IC, Brückner D, Grosser S, Nautiyal R, Rossetti L, Bosch-Padrós M, Trebicka J, Roca-Cusachs P, Sunyer R, Hannezo EB, Trepat X. 2025. Single-cell migration along and against confined haptotactic gradients. Nature Physics. 21, 1638–1647."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"10","type":"journal_article","quality_controlled":"1","language":[{"iso":"eng"}],"OA_place":"repository","department":[{"_id":"EdHa"}],"oa_version":"Preprint","oa":1},{"day":"17","language":[{"iso":"eng"}],"year":"2025","OA_place":"repository","department":[{"_id":"CaHe"},{"_id":"EdHa"}],"license":"https://creativecommons.org/licenses/by-nd/4.0/","oa":1,"oa_version":"Preprint","date_created":"2025-10-14T07:25:27Z","doi":"10.1101/2025.02.14.638262","status":"public","_id":"20465","publication_status":"draft","related_material":{"record":[{"id":"20441","status":"public","relation":"dissertation_contains"}]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.02.14.638262"}],"publisher":"Cold Spring Harbor Laboratory","citation":{"apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &#38; Heisenberg, C.-P. J. (n.d.). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. Cold Spring Harbor Laboratory. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>","ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. bioRxiv, <a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>.","ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>. Cold Spring Harbor Laboratory, n.d. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>.","short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, BioRxiv (n.d.).","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>, Cold Spring Harbor Laboratory, doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>.","ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” <i>bioRxiv</i>. Cold Spring Harbor Laboratory."},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","date_updated":"2026-04-07T11:58:57Z","month":"02","type":"preprint","tmp":{"name":"Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0)","short":"CC BY-ND (4.0)","image":"/image/cc_by_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nd/4.0/legalcode"},"article_processing_charge":"No","date_published":"2025-02-17T00:00:00Z","publication":"bioRxiv","abstract":[{"text":"For tissues to spread, they must be deformable while maintaining their structural integrity. How these opposing requirements are balanced within spreading tissues is not yet well understood. Here, we show that keratin intermediate filaments function in epithelial spreading by adapting tissue mechanical resilience to the stresses arising in the tissue during the spreading process. By analysing the expansion of the enveloping cell layer (EVL) over the large yolk cell in early zebrafish embryos in vivo, we found that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, which is essential for preventing tissue rupture. Interestingly, keratins are also required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the force-generating processes pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production in the yolk cell and the mechanical resilience of the EVL against stresses generated by these pulling forces, thereby ensuring uniform and robust tissue spreading.","lang":"eng"}],"corr_author":"1","author":[{"last_name":"Naik","first_name":"Suyash","orcid":"0000-0001-8421-5508","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","full_name":"Naik, Suyash"},{"first_name":"Yann-Edwin","last_name":"Keta","full_name":"Keta, Yann-Edwin"},{"full_name":"Pranjic-Ferscha, Kornelija","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija","last_name":"Pranjic-Ferscha"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"},{"last_name":"Henkes","first_name":"Silke","full_name":"Henkes, Silke"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties"},{"article_number":"041017","title":"Emergent dynamics of active elastic microbeams","ec_funded":1,"volume":15,"abstract":[{"text":"In equilibrium, the physical properties of matter are set by the interactions between the constituents. In contrast, the energy input of the individual components controls the behavior of synthetic or living active matter. Great progress has been made in understanding the emergent phenomena in active fluids, though their inability to resist shear forces hinders their practical use. This motivates the exploration of active solids as shape-shifting materials, yet, we lack controlled synthetic systems to devise active solids with unconventional properties. Here we build active elastic beams from dozens of active colloids and unveil complex emergent behaviors such as self-oscillations or persistent rotations. Developing tensile tests at the microscale, we show that the active beams are ultrasoft materials, with large (nonequilibrium) fluctuations. Combining experiments, theory, and stochastic inference, we show that the dynamics of the active beams can be mapped on different phase transitions which are tuned by boundary conditions. More quantitatively, we assess all relevant parameters by independent measurements or first-principles calculations, and find that our theoretical description agrees with the experimental observations. Our results demonstrate that the simple addition of activity to an elastic beam unveils novel physics and can inspire design strategies for active solids and functional microscopic machines.","lang":"eng"}],"publication":"Physical Review X","file_date_updated":"2025-12-01T07:30:00Z","external_id":{"arxiv":["2508.20642"]},"article_processing_charge":"Yes","project":[{"name":"VULCAN: matter, powered from within","_id":"bdac72da-d553-11ed-ba76-eae56e802b74","grant_number":"101086998"},{"name":"IST-BRIDGE: International postdoctoral program","call_identifier":"H2020","grant_number":"101034413","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c"}],"arxiv":1,"type":"journal_article","month":"10","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Martinet Q, Li YI, Aubret A, Hannezo EB, Palacci JA. 2025. Emergent dynamics of active elastic microbeams. Physical Review X. 15(4), 041017.","apa":"Martinet, Q., Li, Y. I., Aubret, A., Hannezo, E. B., &#38; Palacci, J. A. (2025). Emergent dynamics of active elastic microbeams. <i>Physical Review X</i>. American Physical Society. <a href=\"https://doi.org/10.1103/rjk2-q2wh\">https://doi.org/10.1103/rjk2-q2wh</a>","ieee":"Q. Martinet, Y. I. Li, A. Aubret, E. B. Hannezo, and J. A. Palacci, “Emergent dynamics of active elastic microbeams,” <i>Physical Review X</i>, vol. 15, no. 4. American Physical Society, 2025.","mla":"Martinet, Quentin, et al. “Emergent Dynamics of Active Elastic Microbeams.” <i>Physical Review X</i>, vol. 15, no. 4, 041017, American Physical Society, 2025, doi:<a href=\"https://doi.org/10.1103/rjk2-q2wh\">10.1103/rjk2-q2wh</a>.","short":"Q. Martinet, Y.I. Li, A. Aubret, E.B. Hannezo, J.A. Palacci, Physical Review X 15 (2025).","chicago":"Martinet, Quentin, Yuting I Li, A. Aubret, Edouard B Hannezo, and Jérémie A Palacci. “Emergent Dynamics of Active Elastic Microbeams.” <i>Physical Review X</i>. American Physical Society, 2025. <a href=\"https://doi.org/10.1103/rjk2-q2wh\">https://doi.org/10.1103/rjk2-q2wh</a>.","ama":"Martinet Q, Li YI, Aubret A, Hannezo EB, Palacci JA. Emergent dynamics of active elastic microbeams. <i>Physical Review X</i>. 2025;15(4). doi:<a href=\"https://doi.org/10.1103/rjk2-q2wh\">10.1103/rjk2-q2wh</a>"},"scopus_import":"1","acknowledgement":"The authors thank Andela Saric, Christoph Zechner, and Paul Robin for helpful discussions. J. P. acknowledges support by ERC grant (VULCAN, 101086998) and U.S. ARO under Award No. W911NF2310008. Y. I. L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 101034413.","_id":"20708","publication_identifier":{"eissn":["2160-3308"]},"doi":"10.1103/rjk2-q2wh","date_created":"2025-11-30T23:02:08Z","oa":1,"oa_version":"Published Version","department":[{"_id":"EdHa"},{"_id":"JePa"}],"DOAJ_listed":"1","OA_place":"publisher","language":[{"iso":"eng"}],"quality_controlled":"1","issue":"4","author":[{"first_name":"Quentin","last_name":"Martinet","orcid":"0000-0002-2916-6632","full_name":"Martinet, Quentin","id":"b37485a8-d343-11eb-a0e9-df8c484ef8ab"},{"last_name":"Li","first_name":"Yuting I","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","full_name":"Li, Yuting I"},{"last_name":"Aubret","first_name":"A.","full_name":"Aubret, A."},{"first_name":"Edouard B","last_name":"Hannezo","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561"},{"id":"8fb92548-2b22-11eb-b7c1-a3f0d08d7c7d","full_name":"Palacci, Jérémie A","orcid":"0000-0002-7253-9465","last_name":"Palacci","first_name":"Jérémie A"}],"corr_author":"1","OA_type":"gold","APC_amount":"4695,11 EUR","date_published":"2025-10-31T00:00:00Z","ddc":["530"],"has_accepted_license":"1","intvolume":"        15","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"date_updated":"2026-05-20T08:58:06Z","article_type":"original","publisher":"American Physical Society","publication_status":"published","status":"public","file":[{"creator":"dernst","content_type":"application/pdf","date_updated":"2025-12-01T07:30:00Z","file_size":5902259,"relation":"main_file","checksum":"bb64ea9f2c400205fd89e9bdd15cc850","date_created":"2025-12-01T07:30:00Z","access_level":"open_access","success":1,"file_name":"2025_PhysicalReviewX_Martinet.pdf","file_id":"20714"}],"year":"2025","PlanS_conform":"1","day":"31"},{"publication_identifier":{"issn":["1534-5807"]},"_id":"18807","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"19763"}]},"date_created":"2025-01-09T11:25:47Z","doi":"10.1016/j.devcel.2024.10.024","acknowledgement":"We thank A. Miller and N. Papalopulu for reagents and J. Briscoe for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European Research Council under Horizon Europe, grant 101044579; and the Austrian Science Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022).","citation":{"short":"S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo, A. Kicheva, Developmental Cell 60 (2025) 567–580.","ieee":"S. Rus <i>et al.</i>, “Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling,” <i>Developmental Cell</i>, vol. 60, no. 4. Elsevier, pp. 567–580, 2025.","mla":"Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>, vol. 60, no. 4, Elsevier, 2025, pp. 567–80, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>.","ama":"Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. 2025;60(4):567-580. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>","chicago":"Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>.","ista":"Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva A. 2025. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.","apa":"Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E. B., &#38; Kicheva, A. (2025). Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>"},"scopus_import":"1","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"02","quality_controlled":"1","language":[{"iso":"eng"}],"issue":"4","OA_place":"publisher","department":[{"_id":"AnKi"},{"_id":"EdHa"},{"_id":"NanoFab"}],"oa_version":"Published Version","oa":1,"file_date_updated":"2025-04-16T10:54:07Z","volume":60,"publication":"Developmental Cell","abstract":[{"lang":"eng","text":"Developing tissues interpret dynamic changes in morphogen activity to generate cell type diversity. To quantitatively study bone morphogenetic protein (BMP) signaling dynamics in the mouse neural tube, we developed an embryonic stem cell differentiation system tailored for growing tissues. Differentiating cells form striking self-organized patterns of dorsal neural tube cell types driven by sequential phases of BMP signaling that are observed both in vitro and in vivo. Data-driven biophysical modeling showed that these dynamics result from coupling fast negative feedback with slow positive regulation of signaling by the specification of an endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling in space, we identify a BMP signaling relay that operates in time. This mechanism allows for a rapid initial concentration-sensitive response that is robustly terminated, thereby regulating balanced sequential cell type generation. Our study provides an experimental and theoretical framework to understand how signaling dynamics are exploited in developing tissues."}],"title":"Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling","project":[{"_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","grant_number":"101044579","name":"Mechanisms of tissue size regulation in spinal cord development"},{"grant_number":"F7802","_id":"059DF620-7A3F-11EA-A408-12923DDC885E","name":"Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen control of growth and pattern in the spinal cord"},{"name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","grant_number":"SC19-011","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A"}],"external_id":{"isi":["001434279000001"],"pmid":["39603235"]},"page":"567-580","article_processing_charge":"Yes (via OA deal)","pmid":1,"publication_status":"published","status":"public","publisher":"Elsevier","article_type":"original","date_updated":"2026-06-27T22:30:31Z","day":"24","year":"2025","file":[{"file_size":6994499,"date_updated":"2025-04-16T10:54:07Z","content_type":"application/pdf","creator":"dernst","access_level":"open_access","date_created":"2025-04-16T10:54:07Z","relation":"main_file","checksum":"bb58db4a908a1f4aabe4004706154541","success":1,"file_id":"19584","file_name":"2025_DevelopmentalCell_Lehr.pdf"}],"date_published":"2025-02-24T00:00:00Z","ddc":["570"],"corr_author":"1","OA_type":"hybrid","author":[{"last_name":"Rus","first_name":"Stefanie","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","full_name":"Rus, Stefanie","orcid":"0000-0001-8703-1093"},{"full_name":"Brückner, David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","orcid":"0000-0001-7205-2975","first_name":"David","last_name":"Brückner"},{"full_name":"Minchington, Thomas","id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f","first_name":"Thomas","last_name":"Minchington"},{"id":"48A59534-F248-11E8-B48F-1D18A9856A87","full_name":"Greunz, Martina","last_name":"Greunz","first_name":"Martina"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","first_name":"Edouard B"},{"last_name":"Kicheva","first_name":"Anna","orcid":"0000-0003-4509-4998","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","full_name":"Kicheva, Anna"}],"isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        60","has_accepted_license":"1"},{"external_id":{"isi":["001154500400001"],"pmid":["38134934"]},"page":"171-182.e8","article_processing_charge":"Yes (via OA deal)","project":[{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"title":"Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts","publication":"Current Biology","abstract":[{"text":"Metazoan development relies on the formation and remodeling of cell-cell contacts. Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in space and time plays a central role in cell-cell contact formation and maturation. Nevertheless, how this process is mechanistically achieved when new contacts are formed remains unclear. Here, by building a biomimetic assay composed of progenitor cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains, we show that cortical F-actin flows, driven by the depletion of myosin-2 at the cell contact center, mediate the dynamic reorganization of adhesion receptors and cell cortex at the contact. E-cadherin-dependent downregulation of the small GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2 becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical tension gradient from the contact rim to its center. This tension gradient, in turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin at the contact rim and the progressive redistribution of E-cadherin from the contact center to the rim. Eventually, this combination of actomyosin downregulation and flows at the contact determines the characteristic molecular organization, with E-cadherin and F-actin accumulating at the contact rim, where they are needed to mechanically link the contractile cortices of the adhering cells.","lang":"eng"}],"ec_funded":1,"volume":34,"file_date_updated":"2024-01-16T10:53:31Z","oa":1,"oa_version":"Published Version","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"NanoFab"}],"issue":"1","quality_controlled":"1","language":[{"iso":"eng"}],"month":"01","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","scopus_import":"1","citation":{"ista":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1), 171–182.e8.","apa":"Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., &#38; Heisenberg, C.-P. J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>","short":"F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current Biology 34 (2024) 171–182.e8.","ieee":"F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg, “Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” <i>Current Biology</i>, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.","mla":"Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>.","ama":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. 2024;34(1):171-182.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>","chicago":"Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>."},"acknowledgement":"We are grateful to Edwin Munro for their feedback and help with the single particle analysis. We thank members of the Heisenberg and Loose labs for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA for their continuous support, especially Yann Cesbron for assistance with the laser cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H.","date_created":"2024-01-14T23:00:56Z","doi":"10.1016/j.cub.2023.11.067","_id":"14795","publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"has_accepted_license":"1","intvolume":"        34","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"author":[{"full_name":"Arslan, Feyza N","id":"49DA7910-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5809-9566","first_name":"Feyza N","last_name":"Arslan"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","last_name":"Hannezo"},{"first_name":"Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-7309-9724","full_name":"Loose, Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","last_name":"Loose"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"corr_author":"1","ddc":["570"],"date_published":"2024-01-08T00:00:00Z","file":[{"file_size":5183861,"date_updated":"2024-01-16T10:53:31Z","content_type":"application/pdf","creator":"dernst","access_level":"open_access","date_created":"2024-01-16T10:53:31Z","relation":"main_file","checksum":"51220b76d72a614208f84bdbfbaf9b72","success":1,"file_id":"14813","file_name":"2024_CurrentBiology_Arslan.pdf"}],"year":"2024","day":"08","date_updated":"2025-09-04T11:39:10Z","publisher":"Elsevier","article_type":"original","status":"public","pmid":1,"publication_status":"published"},{"issue":"4","language":[{"iso":"eng"}],"quality_controlled":"1","department":[{"_id":"EdHa"}],"oa":1,"oa_version":"Published Version","date_created":"2024-06-03T08:58:44Z","doi":"10.21468/scipostphys.16.4.097","_id":"17104","publication_identifier":{"issn":["2542-4653"]},"acknowledgement":"JE and JK gratefully acknowledge financial support from the Initiative and Networking Fund (IVF) via the grant number ERC-RA-004. Simulations were performed with computing resources granted by RWTH Aachen University under project ‘rwth0475’.","scopus_import":"1","citation":{"ista":"Krämer JC, Hannezo EB, Gompper G, Elgeti J. 2024. Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells. SciPost Physics. 16(4), 097.","apa":"Krämer, J. C., Hannezo, E. B., Gompper, G., &#38; Elgeti, J. (2024). Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells. <i>SciPost Physics</i>. SciPost Foundation. <a href=\"https://doi.org/10.21468/scipostphys.16.4.097\">https://doi.org/10.21468/scipostphys.16.4.097</a>","ieee":"J. C. Krämer, E. B. Hannezo, G. Gompper, and J. Elgeti, “Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells,” <i>SciPost Physics</i>, vol. 16, no. 4. SciPost Foundation, 2024.","mla":"Krämer, Johannes C., et al. “Mechanically-Driven Stem Cell Separation in Tissues Caused by Proliferating Daughter Cells.” <i>SciPost Physics</i>, vol. 16, no. 4, 097, SciPost Foundation, 2024, doi:<a href=\"https://doi.org/10.21468/scipostphys.16.4.097\">10.21468/scipostphys.16.4.097</a>.","short":"J.C. Krämer, E.B. Hannezo, G. Gompper, J. Elgeti, SciPost Physics 16 (2024).","chicago":"Krämer, Johannes C., Edouard B Hannezo, Gerhard Gompper, and Jens Elgeti. “Mechanically-Driven Stem Cell Separation in Tissues Caused by Proliferating Daughter Cells.” <i>SciPost Physics</i>. SciPost Foundation, 2024. <a href=\"https://doi.org/10.21468/scipostphys.16.4.097\">https://doi.org/10.21468/scipostphys.16.4.097</a>.","ama":"Krämer JC, Hannezo EB, Gompper G, Elgeti J. Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells. <i>SciPost Physics</i>. 2024;16(4). doi:<a href=\"https://doi.org/10.21468/scipostphys.16.4.097\">10.21468/scipostphys.16.4.097</a>"},"month":"04","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","arxiv":1,"article_processing_charge":"No","external_id":{"arxiv":["2310.04272"],"isi":["001202370200001"]},"file_date_updated":"2024-06-03T11:18:51Z","abstract":[{"text":"The homeostasis of epithelial tissue relies on a balance between the self-renewal of stem cell populations, cellular differentiation, and loss. Although this balance needs to be tightly regulated to avoid pathologies, such as tumor growth, the regulatory mechanisms, both cell-intrinsic and collective, which ensure tissue steady-state are still poorly understood. Here, we develop a computational model that incorporates basic assumptions of stem cell renewal into distinct populations and mechanical interactions between cells. We find that the model generates unexpected dynamic features: stem cells repel each other in the bulk tissue and are thus found rather isolated, as in a number of in vivo contexts. By mapping the system onto a gas of passive Brownian particles with effective repulsive interactions, that arise from the generated flows of differentiated cells, we show that we can quantitatively describe such stem cell distribution in tissues. The interaction potential between a pair of stem cells decays exponentially with a characteristic length that spans several cell sizes, corresponding to the volume of cells generated per stem cell division. Our findings may help understanding the dynamics of normal and cancerous epithelial tissues.","lang":"eng"}],"publication":"SciPost Physics","volume":16,"article_number":"097","title":"Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells","day":"08","year":"2024","file":[{"success":1,"file_id":"17109","file_name":"2024_SciPostPhys_Kraemer.pdf","file_size":4973291,"content_type":"application/pdf","date_updated":"2024-06-03T11:18:51Z","creator":"dernst","access_level":"open_access","relation":"main_file","checksum":"6fdeecd21c166db8dedb927ecc2e6025","date_created":"2024-06-03T11:18:51Z"}],"status":"public","publication_status":"published","publisher":"SciPost Foundation","article_type":"original","date_updated":"2025-09-08T07:45:40Z","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"intvolume":"        16","has_accepted_license":"1","date_published":"2024-04-08T00:00:00Z","ddc":["530"],"author":[{"first_name":"Johannes C.","last_name":"Krämer","full_name":"Krämer, Johannes C."},{"last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Gompper","first_name":"Gerhard","full_name":"Gompper, Gerhard"},{"full_name":"Elgeti, Jens","last_name":"Elgeti","first_name":"Jens"}]},{"intvolume":"       386","isi":1,"author":[{"last_name":"Fabrèges","first_name":"Dimitri","full_name":"Fabrèges, Dimitri"},{"full_name":"Corominas-Murtra, Bernat","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9806-5643","first_name":"Bernat","last_name":"Corominas-Murtra"},{"full_name":"Moghe, Prachiti","last_name":"Moghe","first_name":"Prachiti"},{"first_name":"Alison","last_name":"Kickuth","full_name":"Kickuth, Alison"},{"last_name":"Ichikawa","first_name":"Takafumi","full_name":"Ichikawa, Takafumi"},{"last_name":"Iwatani","first_name":"Chizuru","full_name":"Iwatani, Chizuru"},{"last_name":"Tsukiyama","first_name":"Tomoyuki","full_name":"Tsukiyama, Tomoyuki"},{"full_name":"Daniel, Nathalie","first_name":"Nathalie","last_name":"Daniel"},{"full_name":"Gering, Julie","first_name":"Julie","last_name":"Gering"},{"first_name":"Anniek","last_name":"Stokkermans","full_name":"Stokkermans, Anniek"},{"full_name":"Wolny, Adrian","first_name":"Adrian","last_name":"Wolny"},{"full_name":"Kreshuk, Anna","last_name":"Kreshuk","first_name":"Anna"},{"full_name":"Duranthon, Véronique","first_name":"Véronique","last_name":"Duranthon"},{"full_name":"Uhlman, Virginie","last_name":"Uhlman","first_name":"Virginie"},{"first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hiiragi","first_name":"Takashi","full_name":"Hiiragi, Takashi"}],"OA_type":"green","corr_author":"1","date_published":"2024-10-11T00:00:00Z","year":"2024","day":"11","date_updated":"2025-09-08T14:22:13Z","article_type":"original","publisher":"AAAS","status":"public","pmid":1,"publication_status":"published","external_id":{"pmid":["39388574"],"isi":["001422132300018"]},"article_processing_charge":"No","title":"Temporal variability and cell mechanics control robustness in mammalian embryogenesis","article_number":"eadh1145","abstract":[{"lang":"eng","text":"How living systems achieve precision in form and function despite their intrinsic stochasticity is a fundamental yet ongoing question in biology. We generated morphomaps of preimplantation embryogenesis in mouse, rabbit, and monkey embryos, and these morphomaps revealed that although blastomere divisions desynchronized passively, 8-cell embryos converged toward robust three-dimensional shapes. Using topological analysis and genetic perturbations, we found that embryos progressively changed their cellular connectivity to a preferred topology, which could be predicted by a physical model in which actomyosin contractility and noise facilitate topological transitions, lowering surface energy. This mechanism favored regular embryo packing and promoted a higher number of inner cells in the 16-cell embryo. Synchronized division reduced embryo packing and generated substantially more misallocated cells and fewer inner-cell–mass cells. These findings suggest that stochasticity in division timing contributes to robust patterning."}],"publication":"Science","volume":386,"oa":1,"oa_version":"Submitted Version","department":[{"_id":"EdHa"}],"OA_place":"repository","issue":"6718","language":[{"iso":"eng"}],"quality_controlled":"1","month":"10","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","type":"journal_article","scopus_import":"1","citation":{"ama":"Fabrèges D, Corominas-Murtra B, Moghe P, et al. Temporal variability and cell mechanics control robustness in mammalian embryogenesis. <i>Science</i>. 2024;386(6718). doi:<a href=\"https://doi.org/10.1126/science.adh1145\">10.1126/science.adh1145</a>","chicago":"Fabrèges, Dimitri, Bernat Corominas-Murtra, Prachiti Moghe, Alison Kickuth, Takafumi Ichikawa, Chizuru Iwatani, Tomoyuki Tsukiyama, et al. “Temporal Variability and Cell Mechanics Control Robustness in Mammalian Embryogenesis.” <i>Science</i>. AAAS, 2024. <a href=\"https://doi.org/10.1126/science.adh1145\">https://doi.org/10.1126/science.adh1145</a>.","short":"D. Fabrèges, B. Corominas-Murtra, P. Moghe, A. Kickuth, T. Ichikawa, C. Iwatani, T. Tsukiyama, N. Daniel, J. Gering, A. Stokkermans, A. Wolny, A. Kreshuk, V. Duranthon, V. Uhlman, E.B. Hannezo, T. Hiiragi, Science 386 (2024).","mla":"Fabrèges, Dimitri, et al. “Temporal Variability and Cell Mechanics Control Robustness in Mammalian Embryogenesis.” <i>Science</i>, vol. 386, no. 6718, eadh1145, AAAS, 2024, doi:<a href=\"https://doi.org/10.1126/science.adh1145\">10.1126/science.adh1145</a>.","ieee":"D. Fabrèges <i>et al.</i>, “Temporal variability and cell mechanics control robustness in mammalian embryogenesis,” <i>Science</i>, vol. 386, no. 6718. AAAS, 2024.","apa":"Fabrèges, D., Corominas-Murtra, B., Moghe, P., Kickuth, A., Ichikawa, T., Iwatani, C., … Hiiragi, T. (2024). Temporal variability and cell mechanics control robustness in mammalian embryogenesis. <i>Science</i>. AAAS. <a href=\"https://doi.org/10.1126/science.adh1145\">https://doi.org/10.1126/science.adh1145</a>","ista":"Fabrèges D, Corominas-Murtra B, Moghe P, Kickuth A, Ichikawa T, Iwatani C, Tsukiyama T, Daniel N, Gering J, Stokkermans A, Wolny A, Kreshuk A, Duranthon V, Uhlman V, Hannezo EB, Hiiragi T. 2024. Temporal variability and cell mechanics control robustness in mammalian embryogenesis. Science. 386(6718), eadh1145."},"main_file_link":[{"url":"https://hal.inrae.fr/hal-04447081v1/file/2023.01.24.525420.full.pdf","open_access":"1"}],"acknowledgement":"We are grateful to the members of the Hiiragi laboratory for discussions and comments on the manuscript: R. Bloehs, S. Friese, S. Hozeifi, L. Pérez, and W. Schwarzer for their technical support; V. Janssen for establishing the PAB protocol; members of the Tsukiyama group for the animal care with monkeys, in particular H. Tsuchiya and M. Nakaya; Unité Commune d’Expérimentation Animale (UCEA, Jouy-en-Josas, France) for the animal care with rabbits; the EMBL electronic and mechanical workshops and the EMBL animal facility for their support; We thank Luxendo for the close collaboration in developing the light-sheet microscopy for mammalian embryos.\r\nFunding: This work was funded by the following: EMBL Interdisciplinary Postdoc Program (EIPOD) under Marie Sklodowska Curie Actions COFUND III RTD (to D.F.); JSPS Overseas Research Fellowship (to T.I.); Field of excellence “Complexity of life in basic research and innovation” of the University of Graz (to B.C.M.); European Research Council, ERC Advanced Grant “SelforganisingEmbryo”, grant agreement 742732; ERC Advanced Grant “COORDINATION” grant agreement 101055287 (to T.H.); Stichting LSH-TKI, grant LSHM21020 (to T.H.) JSPS KAKENHI grants JP21H05038 and JP22H05166 (to T.H.)","doi":"10.1126/science.adh1145","date_created":"2024-10-20T22:02:06Z","publication_identifier":{"eissn":["1095-9203"]},"_id":"18446"},{"project":[{"name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288"},{"name":"A mechano-chemical theory for stem cell fate decisions in organoid development","grant_number":"ALTF 343-2022","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"}],"article_processing_charge":"No","external_id":{"isi":["001250246200004"]},"page":"1492-1500","title":"Geometry-driven migration efficiency of autonomous epithelial cell clusters","publication":"Nature Physics","abstract":[{"lang":"eng","text":"The directed migration of epithelial cell collectives through coordinated movements plays a crucial role in various physiological processes and is increasingly understood at the level of large confluent monolayers. However, numerous processes rely on the migration of small groups of polarized epithelial clusters in complex environments, and their responses to external geometries remain poorly understood. To address this, we cultivate primary epithelial keratocyte tissues on adhesive microstripes to create autonomous epithelial clusters with well-defined geometries. We show that their migration efficiency is strongly influenced by the contact geometry and the orientation of cell–cell contacts with respect to the direction of migration. A combination of velocity and polarity alignment with contact regulation of locomotion in an active matter model captures quantitatively the experimental data. Furthermore, we predict that this combination of rules enables efficient navigation in complex geometries, which we confirm experimentally. Altogether, our findings provide a conceptual framework for extracting the interaction rules of active systems from their interaction with physical boundaries, as well as design principles for collective navigation in complex microenvironments."}],"volume":20,"ec_funded":1,"department":[{"_id":"EdHa"}],"oa":1,"oa_version":"Preprint","quality_controlled":"1","language":[{"iso":"eng"}],"OA_place":"repository","scopus_import":"1","citation":{"ista":"Vercruysse E, Brückner D, Gómez-González M, Remson A, Luciano M, Kalukula Y, Rossetti L, Trepat X, Hannezo EB, Gabriele S. 2024. Geometry-driven migration efficiency of autonomous epithelial cell clusters. Nature Physics. 20, 1492–1500.","apa":"Vercruysse, E., Brückner, D., Gómez-González, M., Remson, A., Luciano, M., Kalukula, Y., … Gabriele, S. (2024). Geometry-driven migration efficiency of autonomous epithelial cell clusters. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-024-02532-x\">https://doi.org/10.1038/s41567-024-02532-x</a>","short":"E. Vercruysse, D. Brückner, M. Gómez-González, A. Remson, M. Luciano, Y. Kalukula, L. Rossetti, X. Trepat, E.B. Hannezo, S. Gabriele, Nature Physics 20 (2024) 1492–1500.","ieee":"E. Vercruysse <i>et al.</i>, “Geometry-driven migration efficiency of autonomous epithelial cell clusters,” <i>Nature Physics</i>, vol. 20. Springer Nature, pp. 1492–1500, 2024.","mla":"Vercruysse, Eléonore, et al. “Geometry-Driven Migration Efficiency of Autonomous Epithelial Cell Clusters.” <i>Nature Physics</i>, vol. 20, Springer Nature, 2024, pp. 1492–500, doi:<a href=\"https://doi.org/10.1038/s41567-024-02532-x\">10.1038/s41567-024-02532-x</a>.","ama":"Vercruysse E, Brückner D, Gómez-González M, et al. Geometry-driven migration efficiency of autonomous epithelial cell clusters. <i>Nature Physics</i>. 2024;20:1492-1500. doi:<a href=\"https://doi.org/10.1038/s41567-024-02532-x\">10.1038/s41567-024-02532-x</a>","chicago":"Vercruysse, Eléonore, David Brückner, Manuel Gómez-González, Alexandre Remson, Marine Luciano, Yohalie Kalukula, Leone Rossetti, Xavier Trepat, Edouard B Hannezo, and Sylvain Gabriele. “Geometry-Driven Migration Efficiency of Autonomous Epithelial Cell Clusters.” <i>Nature Physics</i>. Springer Nature, 2024. <a href=\"https://doi.org/10.1038/s41567-024-02532-x\">https://doi.org/10.1038/s41567-024-02532-x</a>."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"09","type":"journal_article","doi":"10.1038/s41567-024-02532-x","date_created":"2024-07-16T12:32:17Z","_id":"17269","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"related_material":{"link":[{"description":"News on ISTA website","relation":"press_release","url":"https://ista.ac.at/en/news/a-railroad-of-cells/"}]},"main_file_link":[{"url":"https://doi.org/10.1101/2022.07.17.500364","open_access":"1"}],"acknowledgement":"M.L., E.V. and S.G. acknowledge funding from the European Regional Development Fund (ERDF) Prostem Research Project (No. 1510614, Wallonia DG06), the Epiforce Project of the National Fund for Scientific Research, Belgium (FRS-FNRS; Project No. T.0092.21), the Cellsqueezer Project of FRS-FNRS (Project No. J.0061.23), the Optopattern Project of FRS-FNRS (Project no. U.NO26.22) and the Interreg MAT(T)ISSE project, which is financially supported by Interreg France-Wallonie-Vlaanderen, ERDF). A.R. and M.L. are financially supported by FRS-FNRS as a research fellow (Aspirant FNRS) and Postdoctoral Researcher (Chargée de Recherches FNRS), respectively. E.V. and Y.K. are financially supported by FRS-FNRS through grants from the Fund for Research Training in Industry and Agriculture (FRIA). This project was supported by the European Research Council under the European Union’s Horizon 2020 Research and Innovation Programme (Grant Agreement No. 851288 to E.H.) and Marie Skłodowska-Curie Actions (Grant Agreement No. 797621 to M.G.-G.). D.B.B. was supported by the NOMIS foundation as a NOMIS fellow and by the European Molecular Biology Organization (Postdoctoral Fellowship ALTF 343-2022) and performed this work in part at the Aspen Center for Physics, which is supported by the National Science Foundation (Grant No. PHY-1607611). X.T. and M.G.-G. acknowledge support from the Government of Catalonia (Grant No. AGAUR SGR-2017-01602 and a CERCA Programme), the Spanish Ministry for Science and Innovation and ERDF (Grant No. PGC2018-099645-B-I00), the European Research Council (Grant No. Adv-883739), Fundació la Marató de TV3 (201903-30-31-32), the European Commission (Grant No. H2020-FETPROACT-01-2016-731957), La Caixa Foundation and the Biomedical Research Center Consortium in Red (Grant No. CB15/00153) at the Carlos III Health Institute, Ministry of Science and Innovation. IBEC is recipient of a Severo Ochoa Award of Excellence from the Spanish Ministry of Economy, Trade and Business.","isi":1,"intvolume":"        20","author":[{"full_name":"Vercruysse, Eléonore","first_name":"Eléonore","last_name":"Vercruysse"},{"last_name":"Brückner","first_name":"David","id":"e1e86031-6537-11eb-953a-f7ab92be508d","full_name":"Brückner, David","orcid":"0000-0001-7205-2975"},{"full_name":"Gómez-González, Manuel","last_name":"Gómez-González","first_name":"Manuel"},{"full_name":"Remson, Alexandre","last_name":"Remson","first_name":"Alexandre"},{"full_name":"Luciano, Marine","first_name":"Marine","last_name":"Luciano"},{"full_name":"Kalukula, Yohalie","last_name":"Kalukula","first_name":"Yohalie"},{"first_name":"Leone","last_name":"Rossetti","full_name":"Rossetti, Leone"},{"first_name":"Xavier","last_name":"Trepat","full_name":"Trepat, Xavier"},{"last_name":"Hannezo","first_name":"Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"},{"last_name":"Gabriele","first_name":"Sylvain","full_name":"Gabriele, Sylvain"}],"date_published":"2024-09-01T00:00:00Z","OA_type":"green","corr_author":"1","day":"01","year":"2024","publisher":"Springer Nature","article_type":"original","date_updated":"2025-09-08T08:28:31Z","status":"public","publication_status":"published"},{"article_type":"review","publisher":"Elsevier","date_updated":"2025-04-14T07:52:27Z","status":"public","publication_status":"published","pmid":1,"file":[{"file_id":"14741","file_name":"2023_SeminarsCellDevBiology_CorominasMurtra.pdf","success":1,"access_level":"open_access","relation":"main_file","date_created":"2024-01-08T10:16:04Z","checksum":"c619887cf130f4649bf3035417186004","file_size":1343750,"content_type":"application/pdf","date_updated":"2024-01-08T10:16:04Z","creator":"dernst"}],"day":"02","year":"2023","author":[{"orcid":"0000-0001-9806-5643","id":"43BE2298-F248-11E8-B48F-1D18A9856A87","full_name":"Corominas-Murtra, Bernat","last_name":"Corominas-Murtra","first_name":"Bernat"},{"first_name":"Edouard B","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"date_published":"2023-12-02T00:00:00Z","ddc":["570"],"corr_author":"1","keyword":["Cell Biology","Developmental Biology"],"has_accepted_license":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"isi":1,"scopus_import":"1","citation":{"ista":"Corominas-Murtra B, Hannezo EB. 2023. Modelling the dynamics of mammalian gut homeostasis. Seminars in Cell &#38; Developmental Biology. 150–151, 58–65.","apa":"Corominas-Murtra, B., &#38; Hannezo, E. B. (2023). Modelling the dynamics of mammalian gut homeostasis. <i>Seminars in Cell &#38; Developmental Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.semcdb.2022.11.005\">https://doi.org/10.1016/j.semcdb.2022.11.005</a>","short":"B. Corominas-Murtra, E.B. Hannezo, Seminars in Cell &#38; Developmental Biology 150–151 (2023) 58–65.","mla":"Corominas-Murtra, Bernat, and Edouard B. Hannezo. “Modelling the Dynamics of Mammalian Gut Homeostasis.” <i>Seminars in Cell &#38; Developmental Biology</i>, vol. 150–151, Elsevier, 2023, pp. 58–65, doi:<a href=\"https://doi.org/10.1016/j.semcdb.2022.11.005\">10.1016/j.semcdb.2022.11.005</a>.","ieee":"B. Corominas-Murtra and E. B. Hannezo, “Modelling the dynamics of mammalian gut homeostasis,” <i>Seminars in Cell &#38; Developmental Biology</i>, vol. 150–151. Elsevier, pp. 58–65, 2023.","ama":"Corominas-Murtra B, Hannezo EB. Modelling the dynamics of mammalian gut homeostasis. <i>Seminars in Cell &#38; Developmental Biology</i>. 2023;150-151:58-65. doi:<a href=\"https://doi.org/10.1016/j.semcdb.2022.11.005\">10.1016/j.semcdb.2022.11.005</a>","chicago":"Corominas-Murtra, Bernat, and Edouard B Hannezo. “Modelling the Dynamics of Mammalian Gut Homeostasis.” <i>Seminars in Cell &#38; Developmental Biology</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.semcdb.2022.11.005\">https://doi.org/10.1016/j.semcdb.2022.11.005</a>."},"month":"12","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","doi":"10.1016/j.semcdb.2022.11.005","date_created":"2023-01-12T12:09:47Z","publication_identifier":{"issn":["1084-9521"]},"_id":"12162","acknowledgement":"This work received funding from the ERC under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 851288 to E.H.).\r\nB. C-M wants to acknowledge the support of the field of excellence Complexity of Life, in Basic Research and Innovation of the University of Graz.","department":[{"_id":"EdHa"}],"oa":1,"oa_version":"Published Version","quality_controlled":"1","language":[{"iso":"eng"}],"title":"Modelling the dynamics of mammalian gut homeostasis","file_date_updated":"2024-01-08T10:16:04Z","abstract":[{"text":"Homeostatic balance in the intestinal epithelium relies on a fast cellular turnover, which is coordinated by an intricate interplay between biochemical signalling, mechanical forces and organ geometry. We review recent modelling approaches that have been developed to understand different facets of this remarkable homeostatic equilibrium. Existing models offer different, albeit complementary, perspectives on the problem. First, biomechanical models aim to explain the local and global mechanical stresses driving cell renewal as well as tissue shape maintenance. Second, compartmental models provide insights into the conditions necessary to keep a constant flow of cells with well-defined ratios of cell types, and how perturbations can lead to an unbalance of relative compartment sizes. A third family of models address, at the cellular level, the nature and regulation of stem fate choices that are necessary to fuel cellular turnover. We also review how these different approaches are starting to be integrated together across scales, to provide quantitative predictions and new conceptual frameworks to think about the dynamics of cell renewal in complex tissues.","lang":"eng"}],"publication":"Seminars in Cell & Developmental Biology","volume":"150-151","ec_funded":1,"project":[{"name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288"}],"external_id":{"pmid":["36470715"],"isi":["001053522200001"]},"page":"58-65","article_processing_charge":"Yes (via OA deal)"},{"pmid":1,"publication_status":"published","status":"public","date_updated":"2024-10-09T21:04:04Z","series_title":"MIMB","publisher":"Springer Nature","year":"2023","day":"19","file":[{"relation":"main_file","checksum":"aec1b8d3ba938ddf9d8fcb777f3c38ee","date_created":"2023-02-03T10:56:39Z","access_level":"open_access","creator":"dernst","file_size":826598,"content_type":"application/pdf","date_updated":"2023-02-03T10:56:39Z","file_name":"2023_MIMB_Hannezo.pdf","file_id":"12500","success":1}],"corr_author":"1","date_published":"2023-01-19T00:00:00Z","ddc":["570"],"author":[{"full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo"},{"first_name":"Colinda L.G.J.","last_name":"Scheele","full_name":"Scheele, Colinda L.G.J."}],"intvolume":"      2608","editor":[{"full_name":"Margadant, Coert","last_name":"Margadant","first_name":"Coert"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","publication_identifier":{"eissn":["1940-6029"],"eisbn":["9781071628874"],"isbn":["9781071628867"]},"_id":"12428","date_created":"2023-01-29T23:00:58Z","doi":"10.1007/978-1-0716-2887-4_12","type":"book_chapter","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"01","citation":{"chicago":"Hannezo, Edouard B, and Colinda L.G.J. Scheele. “A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland.” In <i>Cell Migration in Three Dimensions</i>, edited by Coert Margadant, 2608:183–205. MIMB. Springer Nature, 2023. <a href=\"https://doi.org/10.1007/978-1-0716-2887-4_12\">https://doi.org/10.1007/978-1-0716-2887-4_12</a>.","ama":"Hannezo EB, Scheele CLGJ. A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland. In: Margadant C, ed. <i>Cell Migration in Three Dimensions</i>. Vol 2608. MIMB. Springer Nature; 2023:183-205. doi:<a href=\"https://doi.org/10.1007/978-1-0716-2887-4_12\">10.1007/978-1-0716-2887-4_12</a>","ieee":"E. B. Hannezo and C. L. G. J. Scheele, “A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland,” in <i>Cell Migration in Three Dimensions</i>, vol. 2608, C. Margadant, Ed. Springer Nature, 2023, pp. 183–205.","mla":"Hannezo, Edouard B., and Colinda L. G. J. Scheele. “A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland.” <i>Cell Migration in Three Dimensions</i>, edited by Coert Margadant, vol. 2608, Springer Nature, 2023, pp. 183–205, doi:<a href=\"https://doi.org/10.1007/978-1-0716-2887-4_12\">10.1007/978-1-0716-2887-4_12</a>.","short":"E.B. Hannezo, C.L.G.J. Scheele, in:, C. Margadant (Ed.), Cell Migration in Three Dimensions, Springer Nature, 2023, pp. 183–205.","apa":"Hannezo, E. B., &#38; Scheele, C. L. G. J. (2023). A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland. In C. Margadant (Ed.), <i>Cell Migration in Three Dimensions</i> (Vol. 2608, pp. 183–205). Springer Nature. <a href=\"https://doi.org/10.1007/978-1-0716-2887-4_12\">https://doi.org/10.1007/978-1-0716-2887-4_12</a>","ista":"Hannezo EB, Scheele CLGJ. 2023.A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland. In: Cell Migration in Three Dimensions. Methods in Molecular Biology, vol. 2608, 183–205."},"scopus_import":"1","quality_controlled":"1","language":[{"iso":"eng"}],"oa":1,"oa_version":"Published Version","department":[{"_id":"EdHa"}],"volume":2608,"alternative_title":["Methods in Molecular Biology"],"abstract":[{"lang":"eng","text":"The mammary gland consists of a bilayered epithelial structure with an extensively branched morphology. The majority of this epithelial tree is laid down during puberty, during which actively proliferating terminal end buds repeatedly elongate and bifurcate to form the basic structure of the ductal tree. Mammary ducts consist of a basal and luminal cell layer with a multitude of identified sub-lineages within both layers. The understanding of how these different cell lineages are cooperatively driving branching morphogenesis is a problem of crossing multiple scales, as this requires information on the macroscopic branched structure of the gland, as well as data on single-cell dynamics driving the morphogenic program. Here we describe a method to combine genetic lineage tracing with whole-gland branching analysis. Quantitative data on the global organ structure can be used to derive a model for mammary gland branching morphogenesis and provide a backbone on which the dynamics of individual cell lineages can be simulated and compared to lineage-tracing approaches. Eventually, these quantitative models and experiments allow to understand the couplings between the macroscopic shape of the mammary gland and the underlying single-cell dynamics driving branching morphogenesis."}],"publication":"Cell Migration in Three Dimensions","file_date_updated":"2023-02-03T10:56:39Z","title":"A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Gland","external_id":{"pmid":["36653709"]},"page":"183-205","article_processing_charge":"No"},{"type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"03","citation":{"chicago":"Schamberger, Barbara, Ricardo Ziege, Karine Anselme, Martine Ben Amar, Michał Bykowski, André P.G. Castro, Amaia Cipitria, et al. “Curvature in Biological Systems: Its Quantification, Emergence, and Implications across the Scales.” <i>Advanced Materials</i>. Wiley, 2023. <a href=\"https://doi.org/10.1002/adma.202206110\">https://doi.org/10.1002/adma.202206110</a>.","ama":"Schamberger B, Ziege R, Anselme K, et al. Curvature in biological systems: Its quantification, emergence, and implications across the scales. <i>Advanced Materials</i>. 2023;35(13). doi:<a href=\"https://doi.org/10.1002/adma.202206110\">10.1002/adma.202206110</a>","ieee":"B. Schamberger <i>et al.</i>, “Curvature in biological systems: Its quantification, emergence, and implications across the scales,” <i>Advanced Materials</i>, vol. 35, no. 13. Wiley, 2023.","mla":"Schamberger, Barbara, et al. “Curvature in Biological Systems: Its Quantification, Emergence, and Implications across the Scales.” <i>Advanced Materials</i>, vol. 35, no. 13, 2206110, Wiley, 2023, doi:<a href=\"https://doi.org/10.1002/adma.202206110\">10.1002/adma.202206110</a>.","short":"B. Schamberger, R. Ziege, K. Anselme, M. Ben Amar, M. Bykowski, A.P.G. Castro, A. Cipitria, R.A. Coles, R. Dimova, M. Eder, S. Ehrig, L.M. Escudero, M.E. Evans, P.R. Fernandes, P. Fratzl, L. Geris, N. Gierlinger, E.B. Hannezo, A. Iglič, J.J.K. Kirkensgaard, P. Kollmannsberger, Ł. Kowalewska, N.A. Kurniawan, I. Papantoniou, L. Pieuchot, T.H.V. Pires, L.D. Renner, A.O. Sageman-Furnas, G.E. Schröder-Turk, A. Sengupta, V.R. Sharma, A. Tagua, C. Tomba, X. Trepat, S.L. Waters, E.F. Yeo, A. Roschger, C.M. Bidan, J.W.C. Dunlop, Advanced Materials 35 (2023).","apa":"Schamberger, B., Ziege, R., Anselme, K., Ben Amar, M., Bykowski, M., Castro, A. P. G., … Dunlop, J. W. C. (2023). Curvature in biological systems: Its quantification, emergence, and implications across the scales. <i>Advanced Materials</i>. Wiley. <a href=\"https://doi.org/10.1002/adma.202206110\">https://doi.org/10.1002/adma.202206110</a>","ista":"Schamberger B, Ziege R, Anselme K, Ben Amar M, Bykowski M, Castro APG, Cipitria A, Coles RA, Dimova R, Eder M, Ehrig S, Escudero LM, Evans ME, Fernandes PR, Fratzl P, Geris L, Gierlinger N, Hannezo EB, Iglič A, Kirkensgaard JJK, Kollmannsberger P, Kowalewska Ł, Kurniawan NA, Papantoniou I, Pieuchot L, Pires THV, Renner LD, Sageman-Furnas AO, Schröder-Turk GE, Sengupta A, Sharma VR, Tagua A, Tomba C, Trepat X, Waters SL, Yeo EF, Roschger A, Bidan CM, Dunlop JWC. 2023. Curvature in biological systems: Its quantification, emergence, and implications across the scales. Advanced Materials. 35(13), 2206110."},"scopus_import":"1","acknowledgement":"B.S. and A.R. contributed equally to this work. A.P.G.C. and P.R.F. acknowledge the funding from Fundação para a Ciência e Tecnologia (Portugal), through IDMEC, under LAETA project UIDB/50022/2020. T.H.V.P. acknowledges the funding from Fundação para a Ciência e Tecnologia (Portugal), through Ph.D. Grant 2020.04417.BD. A.S. acknowledges that this work was partially supported by the ATTRACT Investigator Grant (no. A17/MS/11572821/MBRACE, to A.S.) from the Luxembourg National Research Fund. The author thanks Gerardo Ceada for his help in the graphical representations. N.A.K. acknowledges support from the European Research Council (grant 851960) and the Gravitation Program “Materials Driven Regeneration,” funded by the Netherlands Organization for Scientific Research (024.003.013). M.B.A. acknowledges support from the French National Research Agency (grant ANR-201-8-CE1-3-0008 for the project “Epimorph”). G.E.S.T. acknowledges funding by the Australian Research Council through project DP200102593. A.C. acknowledges the funding from the Deutsche Forschungsgemeinschaft (DFG) Emmy Noether Grant CI 203/-2 1, the Spanish Ministry of Science and Innovation (PID2021-123013O-BI00) and the IKERBASQUE Basque Foundation for Science.","publication_identifier":{"issn":["0935-9648"],"eissn":["1521-4095"]},"_id":"12710","doi":"10.1002/adma.202206110","date_created":"2023-03-05T23:01:06Z","oa_version":"Published Version","oa":1,"department":[{"_id":"EdHa"}],"quality_controlled":"1","language":[{"iso":"eng"}],"issue":"13","article_number":"2206110","title":"Curvature in biological systems: Its quantification, emergence, and implications across the scales","volume":35,"publication":"Advanced Materials","abstract":[{"text":"Surface curvature both emerges from, and influences the behavior of, living objects at length scales ranging from cell membranes to single cells to tissues and organs. The relevance of surface curvature in biology is supported by numerous experimental and theoretical investigations in recent years. In this review, first, a brief introduction to the key ideas of surface curvature in the context of biological systems is given and the challenges that arise when measuring surface curvature are discussed. Giving an overview of the emergence of curvature in biological systems, its significance at different length scales becomes apparent. On the other hand, summarizing current findings also shows that both single cells and entire cell sheets, tissues or organisms respond to curvature by modulating their shape and their migration behavior. Finally, the interplay between the distribution of morphogens or micro-organisms and the emergence of curvature across length scales is addressed with examples demonstrating these key mechanistic principles of morphogenesis. Overall, this review highlights that curved interfaces are not merely a passive by-product of the chemical, biological, and mechanical processes but that curvature acts also as a signal that co-determines these processes.","lang":"eng"}],"file_date_updated":"2023-09-26T10:51:56Z","article_processing_charge":"No","external_id":{"pmid":["36461812"],"isi":["000941068900001"]},"date_updated":"2023-09-26T10:56:46Z","publisher":"Wiley","article_type":"review","pmid":1,"publication_status":"published","status":"public","file":[{"success":1,"file_name":"2023_AdvancedMaterials_Schamberger.pdf","file_id":"14373","creator":"dernst","date_updated":"2023-09-26T10:51:56Z","content_type":"application/pdf","file_size":2898063,"relation":"main_file","date_created":"2023-09-26T10:51:56Z","checksum":"5c04d68130e97a0ecd1ca27fbc15a246","access_level":"open_access"}],"year":"2023","day":"29","author":[{"full_name":"Schamberger, Barbara","last_name":"Schamberger","first_name":"Barbara"},{"full_name":"Ziege, Ricardo","first_name":"Ricardo","last_name":"Ziege"},{"last_name":"Anselme","first_name":"Karine","full_name":"Anselme, Karine"},{"full_name":"Ben Amar, Martine","first_name":"Martine","last_name":"Ben Amar"},{"full_name":"Bykowski, Michał","first_name":"Michał","last_name":"Bykowski"},{"first_name":"André P.G.","last_name":"Castro","full_name":"Castro, André P.G."},{"full_name":"Cipitria, Amaia","last_name":"Cipitria","first_name":"Amaia"},{"first_name":"Rhoslyn A.","last_name":"Coles","full_name":"Coles, Rhoslyn A."},{"full_name":"Dimova, Rumiana","first_name":"Rumiana","last_name":"Dimova"},{"first_name":"Michaela","last_name":"Eder","full_name":"Eder, Michaela"},{"last_name":"Ehrig","first_name":"Sebastian","full_name":"Ehrig, Sebastian"},{"full_name":"Escudero, Luis M.","first_name":"Luis M.","last_name":"Escudero"},{"full_name":"Evans, Myfanwy E.","first_name":"Myfanwy E.","last_name":"Evans"},{"last_name":"Fernandes","first_name":"Paulo R.","full_name":"Fernandes, Paulo R."},{"full_name":"Fratzl, Peter","last_name":"Fratzl","first_name":"Peter"},{"first_name":"Liesbet","last_name":"Geris","full_name":"Geris, Liesbet"},{"first_name":"Notburga","last_name":"Gierlinger","full_name":"Gierlinger, Notburga"},{"last_name":"Hannezo","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"full_name":"Iglič, Aleš","first_name":"Aleš","last_name":"Iglič"},{"last_name":"Kirkensgaard","first_name":"Jacob J.K.","full_name":"Kirkensgaard, Jacob J.K."},{"last_name":"Kollmannsberger","first_name":"Philip","full_name":"Kollmannsberger, Philip"},{"last_name":"Kowalewska","first_name":"Łucja","full_name":"Kowalewska, Łucja"},{"full_name":"Kurniawan, Nicholas A.","first_name":"Nicholas A.","last_name":"Kurniawan"},{"first_name":"Ioannis","last_name":"Papantoniou","full_name":"Papantoniou, Ioannis"},{"first_name":"Laurent","last_name":"Pieuchot","full_name":"Pieuchot, Laurent"},{"full_name":"Pires, Tiago H.V.","last_name":"Pires","first_name":"Tiago H.V."},{"full_name":"Renner, Lars D.","last_name":"Renner","first_name":"Lars D."},{"full_name":"Sageman-Furnas, Andrew O.","last_name":"Sageman-Furnas","first_name":"Andrew O."},{"first_name":"Gerd E.","last_name":"Schröder-Turk","full_name":"Schröder-Turk, Gerd E."},{"first_name":"Anupam","last_name":"Sengupta","full_name":"Sengupta, Anupam"},{"full_name":"Sharma, Vikas R.","first_name":"Vikas R.","last_name":"Sharma"},{"full_name":"Tagua, Antonio","last_name":"Tagua","first_name":"Antonio"},{"first_name":"Caterina","last_name":"Tomba","full_name":"Tomba, Caterina"},{"first_name":"Xavier","last_name":"Trepat","full_name":"Trepat, Xavier"},{"full_name":"Waters, Sarah L.","first_name":"Sarah L.","last_name":"Waters"},{"last_name":"Yeo","first_name":"Edwina F.","full_name":"Yeo, Edwina F."},{"full_name":"Roschger, Andreas","last_name":"Roschger","first_name":"Andreas"},{"first_name":"Cécile M.","last_name":"Bidan","full_name":"Bidan, Cécile M."},{"first_name":"John W.C.","last_name":"Dunlop","full_name":"Dunlop, John W.C."}],"ddc":["570"],"date_published":"2023-03-29T00:00:00Z","has_accepted_license":"1","intvolume":"        35","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"}}]