[{"title":"Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo","day":"05","PlanS_conform":"1","language":[{"iso":"eng"}],"_id":"21015","intvolume":"        22","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"],"issnl":[" 1745-2473"]},"file_date_updated":"2026-01-21T08:21:11Z","OA_place":"publisher","date_updated":"2026-04-28T12:55:30Z","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","ddc":["570"],"date_published":"2026-01-05T00:00:00Z","month":"01","external_id":{"oaworkid":["W7118187193"]},"author":[{"orcid":"0000-0002-6425-5788","full_name":"Mishra, Nikhil","first_name":"Nikhil","last_name":"Mishra","id":"C4D70E82-1081-11EA-B3ED-9A4C3DDC885E"},{"full_name":"Li, Yuting I","first_name":"Yuting I","last_name":"Li","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"oa":1,"has_accepted_license":"1","oaworkid":1,"status":"public","department":[{"_id":"EdHa"},{"_id":"CaHe"}],"OA_type":"hybrid","year":"2026","date_created":"2026-01-20T10:12:19Z","citation":{"ista":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. Nature Physics. 22, 139–150.","chicago":"Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>.","ieee":"N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo,” <i>Nature Physics</i>, vol. 22. Springer Nature, pp. 139–150, 2026.","ama":"Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. 2026;22:139-150. doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>","apa":"Mishra, N., Li, Y. I., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (2026). Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03122-1\">https://doi.org/10.1038/s41567-025-03122-1</a>","short":"N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026) 139–150.","mla":"Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” <i>Nature Physics</i>, vol. 22, Springer Nature, 2026, pp. 139–50, doi:<a href=\"https://doi.org/10.1038/s41567-025-03122-1\">10.1038/s41567-025-03122-1</a>."},"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"}],"volume":22,"page":"139-150","oa_version":"Published Version","publication_status":"published","corr_author":"1","scopus_import":"1","publisher":"Springer Nature","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"type":"journal_article","fulldoi":"https://doi.org/10.1038/s41567-025-03122-1","ec_funded":1,"article_processing_charge":"Yes (via OA deal)","quality_controlled":"1","abstract":[{"lang":"eng","text":"Early embryo geometry is one of the most invariant species-specific traits, yet its role in ensuring developmental reproducibility and robustness remains underexplored. Here we show that in zebrafish, the geometry of the fertilized egg—specifically its curvature and volume—serves as a critical initial condition triggering a cascade of events that influence development. The embryo geometry guides patterned asymmetric cell divisions in the blastoderm, generating radial gradients of cell volume and nucleocytoplasmic ratio. These gradients generate mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell cycle periods independently of other cells. We demonstrate that reducing cell autonomy reshapes these waves, emphasizing the instructive role of geometry-derived volume patterns in setting the intrinsic period of the cell cycle oscillator. In addition to organizing cell cycles, early embryo geometry spatially patterns zygotic genome activation at the midblastula transition, a key step in establishing embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation pattern and causes ectopic germ layer specification, underscoring the developmental significance of geometry. Together, our findings reveal a symmetry-breaking function of early embryo geometry in coordinating cell cycle and transcriptional patterning."}],"article_type":"original","project":[{"_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020"},{"call_identifier":"H2020","name":"IST-BRIDGE: International postdoctoral program","grant_number":"101034413","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c"},{"_id":"917c023a-16d5-11f0-9cad-eb5cafc52090","name":"Cytoplasmic self-organization into cell-like compartments as a common guiding principle in early animal development"}],"publication":"Nature Physics","file":[{"access_level":"open_access","content_type":"application/pdf","date_updated":"2026-01-21T08:21:11Z","date_created":"2026-01-21T08:21:11Z","relation":"main_file","success":1,"file_id":"21026","file_name":"2026_NaturePhysics_Mishra.pdf","creator":"dernst","checksum":"0ab7ac2fbcb61a364dba57152db64ed7","file_size":7335694}],"acknowledgement":"We thank N. Petridou (EMBL) for sharing results before publication. N.M. was supported by funding from the European Union’s Horizon 2020 programme under the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project ID 1.844. We would like to thank past and present members of the Heisenberg and Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic, N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript. This research was supported by the Scientific Service Units (SSU) of Institute of Science and Technology Austria through resources provided by the Aquatics Facility, Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab Support Facility (LSF). Open access funding provided by Institute of Science and Technology (IST Austria).","related_material":{"link":[{"description":"News on ISTA website","url":"https://ista.ac.at/en/news/geometry-shapes-life/","relation":"research_data"}]},"doi":"10.1038/s41567-025-03122-1"},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"publication_status":"draft","oa_version":"Preprint","corr_author":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Institute of Science and Technology Austria","type":"preprint","dataavailabilitystatement":"The MATLAB code for image analysis, and the full model code, including all parameter values\r\nand condition-specific settings, are available on GitHub at https://github.com/uday2607/EVL-tension-homeostasis.git.","article_processing_charge":"No","abstract":[{"text":"Tissue tension is a key determinant of tissue shape, and its regulation is essential for both morphogenesis and the maintenance of tissue integrity. During zebrafish embryogenesis, the enveloping layer (EVL) – an epithelial monolayer covering the blastoderm – undergoes extensive spreading that is driven by pulling forces exerted at its margin and more than doubles its surface area. Yet whether and how the EVL actively regulates its tissue tension during this process remains unclear. Here, we show that the EVL maintains constant tissue tension while spreading, and that it achieves this by reducing apical cell contractility in response to the same pulling forces that drive its spreading. We identify a mechanosensitive pathway underlying this response, mediated by the scaffold/adaptor protein Kibra regulating the activity of atypical protein kinase C (aPKC) at the apical domain of EVL cells. Under low mechanical stretch, Kibra forms condensates at the base of actin-based apical projections, where it activates Myosin II to increase apical contractility through aPKC downregulation. As mechanical stretch increases, apical projections disassemble, Kibra condensates dissolve, and aPKC activity rises. Elevated aPKC activity in turn reduces apical contractility by reducing Myosin II activity, thereby maintaining constant tissue tension despite increased mechanical stretch. Together, these findings reveal a mechanosensitive mechanism that enables robust adaptation of tissue tension to changing mechanical stretch, ensuring efficient tissue spreading and morphogenesis.","lang":"eng"}],"project":[{"name":"Keratins in epithelial tissue spreading","grant_number":"PAT 5044023","_id":"8f060199-16d5-11f0-9cad-f3253b266c46"},{"name":"Mechanosensitive signaling activation in the crosstalk between mechanical force and tissuefluidity","grant_number":"LTF 16-2022","_id":"34dd7f3b-11ca-11ed-8bc3-856f2c87f5da"}],"researchdata_availability":"yes","file":[{"access_level":"open_access","content_type":"application/pdf","date_created":"2026-07-13T09:16:20Z","date_updated":"2026-07-13T09:16:20Z","relation":"main_file","file_id":"22283","success":1,"checksum":"66444afd243dce7d383d52d44e8d34a4","file_size":12477675,"file_name":"Main_text_and_figures.pdf","creator":"nhino"},{"relation":"main_file","file_id":"22284","success":1,"file_name":"Supplementary_figures.pdf","checksum":"90bceb34de64ec792c5de117f0890d05","creator":"nhino","file_size":4545901,"access_level":"open_access","content_type":"application/pdf","date_updated":"2026-07-13T09:16:25Z","date_created":"2026-07-13T09:16:25Z"},{"relation":"main_file","success":1,"file_id":"22285","file_name":"Supplementary_Video1.mp4","creator":"nhino","file_size":10349451,"checksum":"9d9ab89c372142f2ffb6c8c625334d7f","access_level":"open_access","content_type":"video/mp4","date_created":"2026-07-13T09:16:28Z","date_updated":"2026-07-13T09:16:28Z"}],"related_material":{"record":[{"id":"21864","status":"public","relation":"earlier_version"}]},"acknowledgement":"We thank all members of the Heisenberg group for discussion and feedback on the manuscript, and the Imaging and Optics Facility, the Life Science Support Facility and the Electron Microscopy Facility of the Institute of Science and Technology Austria (ISTA) for their continued support. We are grateful to M. Sonawane (Tata Institute of Fundamental Research, India) for providing the pCS2-HA-aPKC (PKCι)-V260F (DN) and pCS2-HA-aPKC (PKCι)-A122E (CA) plasmids, and to I. Mayer for the discussion. Molecular graphics and analyses were performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. This research was funded in whole or in part by the Austrian Science Fund (FWF; grant no. PAT5044023) to C.-P.H., and by a JSPS Overseas Research Fellowship and an EMBO Postdoctoral Fellowship (ALTF 16-2022) to N.H.","keyword":["Epithelial spreading","tissue tension","mechanosensation","aPKC","Kibra","zebrafish"],"title":"Apical domain mechanosensation regulates tissue tension homeostasis","day":"14","_id":"22276","language":[{"iso":"eng"}],"file_date_updated":"2026-07-13T09:16:28Z","supplementarymaterial":"yes","das_tickbox":"1","OA_place":"publisher","date_updated":"2026-07-14T07:07:41Z","ddc":["570"],"date_published":"2026-07-14T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"07","author":[{"id":"5299a9ce-7679-11eb-a7bc-d1e62b936307","last_name":"Hino","first_name":"Naoya","full_name":"Hino, Naoya"},{"id":"e3b3eda7-fd4d-11eb-8fd8-c40af7a478b1","last_name":"Kapoor","first_name":"Tushna","full_name":"Kapoor, Tushna"},{"full_name":"Gubbala, Uday R","id":"bb4a0dc4-32c9-11ee-b5ce-a97ceedd5924","last_name":"Gubbala","first_name":"Uday R"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"has_accepted_license":"1","oa":1,"status":"public","department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"GradSch"}],"date_created":"2026-07-13T09:03:26Z","citation":{"apa":"Hino, N., Kapoor, T., Gubbala, U. R., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (n.d.). Apical domain mechanosensation regulates tissue tension homeostasis. Institute of Science and Technology Austria.","short":"N. Hino, T. Kapoor, U.R. Gubbala, E.B. Hannezo, C.-P.J. Heisenberg, (n.d.).","ama":"Hino N, Kapoor T, Gubbala UR, Hannezo EB, Heisenberg C-PJ. Apical domain mechanosensation regulates tissue tension homeostasis.","mla":"Hino, Naoya, et al. <i>Apical Domain Mechanosensation Regulates Tissue Tension Homeostasis</i>. Institute of Science and Technology Austria.","ieee":"N. Hino, T. Kapoor, U. R. Gubbala, E. B. Hannezo, and C.-P. J. Heisenberg, “Apical domain mechanosensation regulates tissue tension homeostasis.” Institute of Science and Technology Austria.","chicago":"Hino, Naoya, Tushna Kapoor, Uday R Gubbala, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Apical Domain Mechanosensation Regulates Tissue Tension Homeostasis.” Institute of Science and Technology Austria, n.d.","ista":"Hino N, Kapoor T, Gubbala UR, Hannezo EB, Heisenberg C-PJ. Apical domain mechanosensation regulates tissue tension homeostasis."},"year":"2026","OA_type":"green"},{"author":[{"first_name":"Suyash","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","last_name":"Naik","full_name":"Naik, Suyash","orcid":"0000-0001-8421-5508"},{"full_name":"Keta, Yann-Edwin","first_name":"Yann-Edwin","last_name":"Keta"},{"full_name":"Pranjic-Ferscha, Kornelija","last_name":"Pranjic-Ferscha","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija"},{"first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Silke","last_name":"Henkes","full_name":"Henkes, Silke"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"oa":1,"has_accepted_license":"1","month":"07","external_id":{"pmid":["42143048"]},"department":[{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EdHa"}],"date_created":"2026-07-29T09:10:35Z","citation":{"ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. 2026. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. Nature Communications. 17, 6499.","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>Nature Communications</i>. Springer Nature, 2026. <a href=\"https://doi.org/10.1038/s41467-026-72366-z\">https://doi.org/10.1038/s41467-026-72366-z</a>.","ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” <i>Nature Communications</i>, vol. 17. Springer Nature, 2026.","ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>Nature Communications</i>. 2026;17. doi:<a href=\"https://doi.org/10.1038/s41467-026-72366-z\">10.1038/s41467-026-72366-z</a>","short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, Nature Communications 17 (2026).","apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &#38; Heisenberg, C.-P. J. (2026). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-026-72366-z\">https://doi.org/10.1038/s41467-026-72366-z</a>","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>Nature Communications</i>, vol. 17, 6499, Springer Nature, 2026, doi:<a href=\"https://doi.org/10.1038/s41467-026-72366-z\">10.1038/s41467-026-72366-z</a>."},"OA_type":"gold","year":"2026","status":"public","day":"17","PlanS_conform":"1","pmid":1,"_id":"22608","language":[{"iso":"eng"}],"title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties","ddc":["570"],"date_published":"2026-07-17T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-07-29T10:33:31Z","file_date_updated":"2026-07-29T10:27:25Z","intvolume":"        17","supplementarymaterial":"yes","publication_identifier":{"eissn":["2041-1723"]},"das_tickbox":"1","OA_place":"publisher","abstract":[{"text":"For tissues to spread, they must deform while staying intact. How spreading tissues balance flexibility with integrity is not yet well understood. Here, we show that keratin intermediate filaments adapt tissue mechanical resilience to the stresses arising in epithelial tissues during spreading. By analyzing the expansion of the enveloping cell layer (EVL) over the yolk cell in zebrafish embryos in vivo, we find that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, to prevent tissue rupture. Further, keratins are required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the forces pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production and EVL mechanical resilience, ensuring uniform and robust tissue spreading.","lang":"eng"}],"article_type":"original","researchdata_availability":"yes","project":[{"name":"Keratins in epithelial tissue spreading","grant_number":"PAT 5044023","_id":"8f060199-16d5-11f0-9cad-f3253b266c46"},{"call_identifier":"FWF","_id":"252C3B08-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","grant_number":"W1250-B20"}],"publication":"Nature Communications","article_processing_charge":"Yes","quality_controlled":"1","acknowledgement":"We thank all members of the Heisenberg, Henkes, and Hannezo groups for their support. We are also grateful to the Imaging and Optics, Scientific Computing, Life Science Support, and Cryo-Electron Microscopy facilities at ISTA for their technical assistance and support. Numerical simulations were performed using the computational resources from Lorentz Institute and the Academic Leiden Interdisciplinary Cluster Environment (ALICE) provided by Leiden University, and from PMMH provided by Sorbonne Université. S.N has received funding from European Union’s Horizon 2020 research and innovation programme (grant agreement No. 665385). This work was supported by the Austrian Science Fund (FWF) under projects PAT5044023 and W1250 awarded to C.-P.H.","related_material":{"record":[{"relation":"earlier_version","status":"public","id":"20465"}]},"doi":"10.1038/s41467-026-72366-z","file":[{"relation":"main_file","file_id":"22609","success":1,"file_name":"2026_NatureComm_Naik.pdf","checksum":"f26d96e180c1d034d9c9c8f57c3c258b","creator":"dernst","file_size":15363936,"access_level":"open_access","content_type":"application/pdf","date_updated":"2026-07-29T10:27:25Z","date_created":"2026-07-29T10:27:25Z"}],"publication_status":"published","oa_version":"Published Version","corr_author":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"EM-Fac"}],"article_number":"6499","volume":17,"fulldoi":"https://doi.org/10.1038/s41467-026-72366-z","type":"journal_article","dataavailabilitystatement":"The authors declare that the minimum dataset that is necessary to\r\ninterpret, verify, and extend the research in this article is included in\r\nthe supplementary information, the source data, and the archived data\r\nrepository (https://doi.org/10.15479/AT-ISTA-21137). This is also available\r\non GitHub at https://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data. Source data are provided\r\nwith this paper. The framework to develop the vertex models used in this paper are\r\navailable online on GitHub and archived in the source data provided.\r\nCustom scripts used for analysis of imaging and simulation data are\r\nprovided along with data files for all panels in the source data for this\r\nmanuscript on GitHub and in data repo (https://doi.org/10.15479/ATISTA-\r\n21137). Framework for the vertex model is available at https://\r\ngithub.com/yketta/cells. Code for analysis is available on GitHub\r\nhttps://github.com/Suyash-Naik/2026-\r\nKeratinepithlialspreadingcoordinate-Data.","scopus_import":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Springer Nature"},{"file_date_updated":"2026-10-01T08:34:11Z","intvolume":"       189","publication_identifier":{"issn":["0092-8674"],"eissn":["1097-4172"]},"supplementarymaterial":"yes","OA_place":"publisher","das_tickbox":"1","date_updated":"2026-10-01T08:42:00Z","ddc":["570"],"date_published":"2026-08-20T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","keyword":["tissue patterning","heterogeneity","mechanics","intestinal regeneration","epigenetic competence","multiscale integration","modeling","organoids","image-based phenotyping","Yap1"],"title":"Multiscale integration of tissue and chromatin context converts cell heterogeneity into stable intestinal patterning","day":"20","pmid":1,"issue":"17","language":[{"iso":"eng"}],"_id":"22956","status":"public","date_created":"2026-09-17T11:06:12Z","citation":{"ieee":"C. Schwayer <i>et al.</i>, “Multiscale integration of tissue and chromatin context converts cell heterogeneity into stable intestinal patterning,” <i>Cell</i>, vol. 189, no. 17. Cell Press, p. 5266–5282.e17, 2026.","chicago":"Schwayer, Cornelia, Silvia Barbiero, David Brückner, Koen C. Oost, Clara Baader, Nicole A. Repina, Jinse Kim, et al. “Multiscale Integration of Tissue and Chromatin Context Converts Cell Heterogeneity into Stable Intestinal Patterning.” <i>Cell</i>. Cell Press, 2026. <a href=\"https://doi.org/10.1016/j.cell.2026.06.009\">https://doi.org/10.1016/j.cell.2026.06.009</a>.","ista":"Schwayer C, Barbiero S, Brückner D, Oost KC, Baader C, Repina NA, Kim J, Diaz OE, Uccelli I, Capolupo L, Meylan LC, Kalck V, Moos F, Suppinger S, Yang Q, Schnabl J, Kilik U, Bourdon M, Camp JG, Stockinger B, Ferrari A, Bühler M, Stadler MB, Hannezo EB, Liberali P. 2026. Multiscale integration of tissue and chromatin context converts cell heterogeneity into stable intestinal patterning. Cell. 189(17), 5266–5282.e17.","mla":"Schwayer, Cornelia, et al. “Multiscale Integration of Tissue and Chromatin Context Converts Cell Heterogeneity into Stable Intestinal Patterning.” <i>Cell</i>, vol. 189, no. 17, Cell Press, 2026, p. 5266–5282.e17, doi:<a href=\"https://doi.org/10.1016/j.cell.2026.06.009\">10.1016/j.cell.2026.06.009</a>.","apa":"Schwayer, C., Barbiero, S., Brückner, D., Oost, K. C., Baader, C., Repina, N. A., … Liberali, P. (2026). Multiscale integration of tissue and chromatin context converts cell heterogeneity into stable intestinal patterning. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2026.06.009\">https://doi.org/10.1016/j.cell.2026.06.009</a>","short":"C. Schwayer, S. Barbiero, D. Brückner, K.C. Oost, C. Baader, N.A. Repina, J. Kim, O.E. Diaz, I. Uccelli, L. Capolupo, L.C. Meylan, V. Kalck, F. Moos, S. Suppinger, Q. Yang, J. Schnabl, U. Kilik, M. Bourdon, J.G. Camp, B. Stockinger, A. Ferrari, M. Bühler, M.B. Stadler, E.B. Hannezo, P. Liberali, Cell 189 (2026) 5266–5282.e17.","ama":"Schwayer C, Barbiero S, Brückner D, et al. Multiscale integration of tissue and chromatin context converts cell heterogeneity into stable intestinal patterning. <i>Cell</i>. 2026;189(17):5266-5282.e17. doi:<a href=\"https://doi.org/10.1016/j.cell.2026.06.009\">10.1016/j.cell.2026.06.009</a>"},"OA_type":"hybrid","year":"2026","month":"08","external_id":{"pmid":["42379166"]},"author":[{"full_name":"Schwayer, Cornelia","orcid":"0000-0001-5130-2226","last_name":"Schwayer","id":"3436488C-F248-11E8-B48F-1D18A9856A87","first_name":"Cornelia"},{"first_name":"Silvia","last_name":"Barbiero","full_name":"Barbiero, Silvia"},{"full_name":"Brückner, David","orcid":"0000-0001-7205-2975","first_name":"David","last_name":"Brückner","id":"e1e86031-6537-11eb-953a-f7ab92be508d"},{"first_name":"Koen C.","last_name":"Oost","full_name":"Oost, Koen C."},{"full_name":"Baader, Clara","last_name":"Baader","first_name":"Clara"},{"first_name":"Nicole A.","last_name":"Repina","full_name":"Repina, Nicole A."},{"full_name":"Kim, Jinse","first_name":"Jinse","last_name":"Kim"},{"full_name":"Diaz, Oscar E.","last_name":"Diaz","first_name":"Oscar E."},{"full_name":"Uccelli, Isa","first_name":"Isa","last_name":"Uccelli"},{"first_name":"Laura","last_name":"Capolupo","full_name":"Capolupo, Laura"},{"full_name":"Meylan, Ludivine Challet","last_name":"Meylan","first_name":"Ludivine Challet"},{"first_name":"Veronique","last_name":"Kalck","full_name":"Kalck, Veronique"},{"full_name":"Moos, Franziska","last_name":"Moos","first_name":"Franziska"},{"full_name":"Suppinger, Simon","first_name":"Simon","last_name":"Suppinger"},{"full_name":"Yang, Qiutan","last_name":"Yang","first_name":"Qiutan"},{"last_name":"Schnabl","first_name":"Jakob","full_name":"Schnabl, Jakob"},{"last_name":"Kilik","first_name":"Umut","full_name":"Kilik, Umut"},{"full_name":"Bourdon, Matthieu","first_name":"Matthieu","last_name":"Bourdon"},{"full_name":"Camp, J. Gray","last_name":"Camp","first_name":"J. Gray"},{"last_name":"Stockinger","first_name":"Brigitta","full_name":"Stockinger, Brigitta"},{"full_name":"Ferrari, Alessandra","last_name":"Ferrari","first_name":"Alessandra"},{"last_name":"Bühler","first_name":"Marc","full_name":"Bühler, Marc"},{"first_name":"Michael B.","last_name":"Stadler","full_name":"Stadler, Michael B."},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B"},{"full_name":"Liberali, Prisca","last_name":"Liberali","first_name":"Prisca"}],"has_accepted_license":"1","oa":1,"scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"publisher":"Cell Press","fulldoi":"https://doi.org/10.1016/j.cell.2026.06.009","ec_funded":1,"type":"journal_article","dataavailabilitystatement":"All sequencing data have been deposited at the Gene Expression Omnibus (GEO) under accession number GEO: GSE283662.\r\nAnalysis of imaging and sequencing data was developed in the Liberali lab and is available at https://github.com/LiberaliLab/symmetry-breaking-2026. Detailed information on the packages used is provided in the STAR Methods section.\r\nAny additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","page":"5266-5282.e17","volume":189,"publication_status":"published","oa_version":"Published Version","corr_author":"1","file":[{"relation":"main_file","success":1,"file_id":"23014","file_size":15536364,"file_name":"2026_Cell_Schwayer.pdf","creator":"dernst","checksum":"3ff0f4f8be0e79940ba11b18af318ae3","access_level":"open_access","content_type":"application/pdf","date_updated":"2026-10-01T08:34:11Z","date_created":"2026-10-01T08:34:11Z"}],"acknowledgement":"We thank the Liberali group, M. Verbeek, and C. Soneson for fruitful discussions; E. Tagliavini for IT support; J. Lüthi for Fractal support; H. Köhler for FACS sorting; T.O. Buchholz for help on cell segmentation; B. Treutlein and A. Xavier da Silveira dos Santos for help with library preparation of single-cell ATAC-seq; M. Garioni for help with mouse salivary organoids; M. Kahnwald for help with human intestinal organoids; M. Lütolf, M. Nikolaev, and O. Mitrofanova for providing material and initial training for microwell experiments; J. Tchorz and V. Orsini for intestinal tissue sections of the YAP1 activator experiment in homeostasis; R. Kageyama for DLL1-Venus-T2A-mCherry mice; J.-F. Spetz for technical assistance; C. Lambert and N. Accart for help with sample preparation; Claudia Flandoli for graphic design of schemes; and Zuzana Nascakova for the graphical abstract. We thank the Foundation Hubrecht Organoid Technology (now Foundation Hubrecht Organoid Biobank, https://www.hubrechtorganoidbiobank.org/) for the duodenal organoid line used in this study (HUB-04-A2-001). We thank C.P. Heisenberg, M. Lütolf, C. Tsiairis, J. Zaugg, G. D’Angelo, L. Giorgetti, S. Arber, and the Liberali group for reading the manuscript. C.S. was supported by the European Molecular Biology Organization (Postdoctoral Fellowship ALTF 660-2020) and the Human Frontier Science Program (HFSP) LT000746/2021-L. D.B.B. was supported by the NOMIS Foundation as a NOMIS fellow, an EMBO Postdoctoral Fellowship (ALTF 343-2022), and the Austrian Academy of Sciences through an APART-MINT Fellowship. B.S. was supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK, the UK Medical Research Council, and the Wellcome Trust (FC001159). This work received funding from ERC to E.H. (grant number 851288) and to P.L. (grant number 758617) and from SNSF (310030_208162 and SNSF ERC TMCG-3_223263).","doi":"10.1016/j.cell.2026.06.009","article_processing_charge":"Yes (in subscription journal)","quality_controlled":"1","abstract":[{"text":"Tissue regeneration requires de novo patterning, which has been proposed to be facilitated by cellular heterogeneity. Yet how such heterogeneities are integrated with the mechanochemical state of the tissue and stabilized at the chromatin level into stable, spatially organized fates remains poorly understood. Using in vivo mouse intestinal regeneration models and organoids, we identify a critical density regime that produces a permissive window for heterogeneity in the mechanosensor Yes-associated protein 1 (YAP1). We show that YAP1 heterogeneity is coupled to lineage-biased chromatin accessibility and is decoded through FOXA1, which integrates the permissive chromatin state to Delta-Notch supracellular feedback and lineage commitment. This circuit generates fate bistability and preserves a memory of transient YAP1 activity, thereby maintaining spatial patterning as tissues return to homeostasis after injury. Together, our findings establish a multiscale framework in which tissue-scale mechanics tune single-cell competence and, through FOXA1-mediated bistability, convert transient heterogeneity into stable and self-organized tissue architecture.","lang":"eng"}],"article_type":"original","publication":"Cell","researchdata_availability":"yes","project":[{"name":"A mechano-chemical theory for stem cell fate decisions in organoid development","grant_number":"ALTF 343-2022","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b"},{"call_identifier":"H2020","name":"Design Principles of Branching Morphogenesis","grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E"}]},{"department":[{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"GradSch"},{"_id":"AnSa"},{"_id":"NanoFab"}],"year":"2026","OA_type":"gold","date_created":"2026-09-20T22:01:48Z","citation":{"mla":"Dunajova, Zuzana, et al. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>Science Advances</i>, vol. 12, no. 37, eaed0587, AAAS, 2026, doi:<a href=\"https://doi.org/10.1126/sciadv.aed0587\">10.1126/sciadv.aed0587</a>.","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, R.P. Jenkins, E. Sahai, M.K. Sixt, E.B. Hannezo, Science Advances 12 (2026).","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Jenkins, R. P., Sahai, E., … Hannezo, E. B. (2026). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.aed0587\">https://doi.org/10.1126/sciadv.aed0587</a>","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>Science Advances</i>. 2026;12(37). doi:<a href=\"https://doi.org/10.1126/sciadv.aed0587\">10.1126/sciadv.aed0587</a>","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening,” <i>Science Advances</i>, vol. 12, no. 37. AAAS, 2026.","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Robert P. Jenkins, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>Science Advances</i>. AAAS, 2026. <a href=\"https://doi.org/10.1126/sciadv.aed0587\">https://doi.org/10.1126/sciadv.aed0587</a>.","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Jenkins RP, Sahai E, Sixt MK, Hannezo EB. 2026. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. Science Advances. 12(37), eaed0587."},"status":"public","author":[{"id":"4B39F286-F248-11E8-B48F-1D18A9856A87","last_name":"Dunajova","first_name":"Zuzana","full_name":"Dunajova, Zuzana"},{"orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","last_name":"Tasciyan","first_name":"Saren"},{"full_name":"Majek, Juraj","last_name":"Majek","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","first_name":"Juraj"},{"orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack"},{"first_name":"Robert P.","last_name":"Jenkins","full_name":"Jenkins, Robert P."},{"first_name":"Erik","last_name":"Sahai","full_name":"Sahai, Erik"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"}],"has_accepted_license":"1","oa":1,"month":"09","external_id":{"pmid":["42715298"]},"ddc":["570"],"date_updated":"2026-10-06T11:36:34Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2026-09-09T00:00:00Z","intvolume":"        12","supplementarymaterial":"no","publication_identifier":{"eissn":["2375-2548"]},"file_date_updated":"2026-10-06T11:30:48Z","das_tickbox":"1","OA_place":"publisher","issue":"37","pmid":1,"PlanS_conform":"1","day":"09","language":[{"iso":"eng"}],"_id":"22963","title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening","acknowledgement":"We thank all the members of the Hannezo and Sixt groups for fruitful discussions and t. Kato for advice on the experimental setup. We are also thankful for support from the Scientific Service Units (SSU) of IST Austria through resources provided by the imaging and Optics Facility (iOF) and the Lab Support Facility (lSF). We used chatGPt (GPt- 5,OpenAi, 2025) to make minor grammatical and stylistic revisions to the original manuscript, which was written by us. We carefully reviewed and verified all changes and are solely responsible for all scientific content, data, interpretation, and conclusions. Funding: Z.D. has received funding from the Doctoral Programme of the Austrian Academy of Sciences (OeAW):grant agreement 26360. this work was supported by the European Research council (grant ERC- SyG 101071793 to M.S.). E.S. and R.P.J. are supported by the Francis Crick Institute, which receives its core funding from cancer Research UK (cc2040), the UK Medical Research council(cc2040), and the Wellcome trust (cc2040) and the European Research council (ERC Advanced Grant cAn_ORGAniSe, grant agreement number 101019366).","related_material":{"record":[{"relation":"earlier_version","status":"public","id":"21427"}]},"doi":"10.1126/sciadv.aed0587","file":[{"access_level":"open_access","content_type":"application/pdf","date_updated":"2026-10-06T11:30:48Z","date_created":"2026-10-06T11:30:48Z","relation":"main_file","success":1,"file_id":"23062","file_size":11691064,"file_name":"2026_ScienceAdv_Dunajova.pdf","checksum":"79f419b659650a39b3dfa58c782690a8","creator":"dernst"}],"abstract":[{"lang":"eng","text":"Although studies of tumor invasion have mainly emphasized the evolution of genetic and epigenetic heterogeneity, cellular behaviors also depend on the physical microenvironment. Yet, the tumor microenvironment is both complex and continuously remodeled, making it hard to rigorously test its causal influence on tumor dissemination. Here, we adopted a reductionist approach to studying tumor cell invasion in microengineered environments of tunable patterned geometries. We find that not only the presence of obstacles but more unexpectedly their spatial disorder causes a drastic shift from a collective to a single-cell mode of invasion—comparable in strength to loss of cell-cell adhesion. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell dissemination results both from local geometrical constraints and a global integration of spatial disorder over time. We identify generic universality classes of invasion dynamics dependent on environmental geometry, enabling us to identify quantitative physical principles for tumor dissemination."}],"article_type":"original","researchdata_availability":"yes","project":[{"_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","grant_number":"26360","name":"Motile active matter models of migrating cells and chiral filaments"},{"_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd","grant_number":"101071793","name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces"}],"publication":"Science Advances","article_processing_charge":"Yes","quality_controlled":"1","type":"journal_article","DOAJ_listed":"1","fulldoi":"https://doi.org/10.1126/sciadv.aed0587","dataavailabilitystatement":" All data and code needed to evaluate and reproduce the results in the paper are present in the paper and/or the Supplementary Materials and the associated data and code repositories. Source data underlying all figures and supplementary figures have been deposited in a Zenodo repository (DOi: 10.5281/zenodo.21718217) and will be made publicly available upon publication. custom simulation and analysis codes used in this study are available at https://codeocean.com/capsule/1237446/tree. no unique biological materials were generated in this study. Microfluidic mask designs generated for this work are available from the corresponding authors upon reasonable request.","scopus_import":"1","publisher":"AAAS","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa_version":"Published Version","publication_status":"published","corr_author":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"volume":12,"article_number":"eaed0587"},{"department":[{"_id":"EdHa"}],"OA_type":"hybrid","year":"2025","date_created":"2025-03-09T23:01:28Z","citation":{"chicago":"Xue, Shi-lei, Qiutan Yang, Prisca Liberali, and Edouard B Hannezo. “Mechanochemical Bistability of Intestinal Organoids Enables Robust Morphogenesis.” <i>Nature Physics</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41567-025-02792-1\">https://doi.org/10.1038/s41567-025-02792-1</a>.","ista":"Xue S, Yang Q, Liberali P, Hannezo EB. 2025. Mechanochemical bistability of intestinal organoids enables robust morphogenesis. Nature Physics. 21, 078104.","ieee":"S. Xue, Q. Yang, P. Liberali, and E. B. Hannezo, “Mechanochemical bistability of intestinal organoids enables robust morphogenesis,” <i>Nature Physics</i>, vol. 21. Springer Nature, 2025.","ama":"Xue S, Yang Q, Liberali P, Hannezo EB. Mechanochemical bistability of intestinal organoids enables robust morphogenesis. <i>Nature Physics</i>. 2025;21. doi:<a href=\"https://doi.org/10.1038/s41567-025-02792-1\">10.1038/s41567-025-02792-1</a>","short":"S. Xue, Q. Yang, P. Liberali, E.B. Hannezo, Nature Physics 21 (2025).","apa":"Xue, S., Yang, Q., Liberali, P., &#38; Hannezo, E. B. (2025). Mechanochemical bistability of intestinal organoids enables robust morphogenesis. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-02792-1\">https://doi.org/10.1038/s41567-025-02792-1</a>","mla":"Xue, Shi-lei, et al. “Mechanochemical Bistability of Intestinal Organoids Enables Robust Morphogenesis.” <i>Nature Physics</i>, vol. 21, 078104, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41567-025-02792-1\">10.1038/s41567-025-02792-1</a>."},"status":"public","author":[{"full_name":"Xue, Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","last_name":"Xue","first_name":"Shi-lei"},{"full_name":"Yang, Qiutan","last_name":"Yang","first_name":"Qiutan"},{"full_name":"Liberali, Prisca","last_name":"Liberali","first_name":"Prisca"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"}],"has_accepted_license":"1","oa":1,"month":"02","external_id":{"arxiv":["2403.19900"],"isi":["001434072800001"],"pmid":["40248571"]},"date_published":"2025-02-28T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","ddc":["530"],"date_updated":"2025-09-30T10:47:36Z","intvolume":"        21","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"file_date_updated":"2025-08-05T12:12:03Z","OA_place":"publisher","pmid":1,"isi":1,"PlanS_conform":"1","day":"28","_id":"19373","language":[{"iso":"eng"}],"arxiv":1,"title":"Mechanochemical bistability of intestinal organoids enables robust morphogenesis","acknowledgement":"We thank all members of the Hannezo and Liberali groups for fruitful discussions, as well as C. Schwayer, G. Quintas, L. Capolupo, D. Bruckner and D. Pinheiro for reading the manuscript. We also thank Y. Wu and X. Wu from the Yang group for performing experiments in the last rounds of revision and the So group at the National Institute of Biological Sciences, Beijing, for helping with the light-sheet time-lapse experiments. This work received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme via grant agreement no. 758617 (to P.L.), Swiss National Foundation (SNF) (no. POOP3_157531 to P.L.), the ERC under the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 851288 (to E.H.) and the Austrian Science Fund (FWF) (no. P 31639 to E.H.). This work was supported by the National Natural Science Foundation of China via grant no.3247060387 (to Q.Y.) and the Strategic Priority Research Program of the Chinese Academy of Sciences (no. XDB0820000 to Q.Y.) . Open access funding provided by Institute of Science and Technology (IST Austria).","doi":"10.1038/s41567-025-02792-1","file":[{"date_created":"2025-08-05T12:12:03Z","date_updated":"2025-08-05T12:12:03Z","access_level":"open_access","content_type":"application/pdf","success":1,"file_id":"20129","file_size":16302436,"creator":"dernst","file_name":"2025_NaturePhysics_Xue.pdf","checksum":"fb5e59be145b95f9851d3d7c9dbb85e6","relation":"main_file"}],"abstract":[{"text":"Reproducible pattern and form generation during embryogenesis is poorly understood. Intestinal organoid morphogenesis involves a number of mechanochemical regulators such as cell-type-specific cytoskeletal forces and osmotically driven lumen volume changes. It is unclear how these forces are coordinated in time and space to ensure robust morphogenesis. Here we show how mechanosensitive feedback on cytoskeletal tension gives rise to morphological bistability in a minimal model of organoid morphogenesis. In the model, lumen volume changes can impact the epithelial shape via both direct mechanical and indirect mechanosensitive mechanisms. We find that both bulged and budded crypt states are possible and dependent on the history of volume changes. We test key modelling assumptions via biophysical and pharmacological experiments to demonstrate how bistability can explain experimental observations, such as the importance of the timing of lumen shrinkage and robustness of the final morphogenetic state to mechanical perturbations. This suggests that bistability arising from feedback between cellular tensions and fluid pressure could be a general mechanism that coordinates multicellular shape changes in developing systems.","lang":"eng"}],"article_type":"original","publication":"Nature Physics","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288","name":"Design Principles of Branching Morphogenesis"},{"call_identifier":"FWF","name":"Active mechano-chemical description of the cell cytoskeleton","grant_number":"P31639","_id":"268294B6-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"Yes (via OA deal)","quality_controlled":"1","type":"journal_article","ec_funded":1,"fulldoi":"https://doi.org/10.1038/s41567-025-02792-1","scopus_import":"1","publisher":"Springer Nature","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa_version":"Published Version","publication_status":"published","corr_author":"1","volume":21,"article_number":"078104"},{"scopus_import":"1","publisher":"Elsevier","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"type":"journal_article","fulldoi":"https://doi.org/10.1016/j.jid.2025.01.034","volume":145,"page":"2191-2202.e5","oa_version":"Published Version","publication_status":"published","corr_author":"1","file":[{"file_id":"20874","success":1,"file_name":"2025_JourInvestigativeDerma_Andersen.pdf","creator":"dernst","file_size":7301679,"checksum":"a2b313de3cacb53f20f2b91c42612ad9","relation":"main_file","date_created":"2025-12-29T14:13:01Z","date_updated":"2025-12-29T14:13:01Z","access_level":"open_access","content_type":"application/pdf"}],"acknowledgement":"We thank the members of the Jensen Laboratory for experimental and technical advice, the imaging facilities at reNEW, and animal caretakers for expert assistance. This work was supported by the Lundbeck Foundation (R105-A9755 to KBJ) and the Leo Pharma Foundation (LF-OC-20-000169). The Novo Nordisk Foundation Center for Stem Cell Medicine was supported by a Novo Nordisk Foundation grant (NNF21CC0073729). B.D.S. was supported by the Wellcome Trust (219478/Z/19/Z) and a Royal Society EP Abraham Research Professorship (RP/R1/180165 and RP\\R\\231004). Figure elements were adapted from Bio-Render. KBJ is the lead contact and guarantor of this study.","doi":"10.1016/j.jid.2025.01.034","article_processing_charge":"No","quality_controlled":"1","abstract":[{"lang":"eng","text":"The epidermis provides a protective barrier against hostile environments. However, our knowledge of how this barrier forms during development and is subsequently maintained remains incomplete. The infundibulum is a cylindrical epidermal tissue compartment that serves as an outlet for hair follicles protruding from the skin and the excretion of the sebaceous glands that are essential for proper skin function. In this study, we applied quantitative fate mapping to address how infundibulum are maintained during adulthood. We demonstrate that progenitors build and maintain tissues through stochastic cell fate choices. Long-term analysis identified a preferential transient contribution from cells initially located at the bottom of the structure to the maintenance of the tissue, with bursts of local progenitor expansion associated with the phases of hair growth. Beyond providing compartment-wide insights into progenitor cell dynamics in infundibulum, these findings demonstrate how spatiotemporal regulation controls transient progenitor dominance."}],"article_type":"original","publication":"Journal of Investigative Dermatology","publication_identifier":{"issn":["0022-202X"],"eissn":["1523-1747"]},"intvolume":"       145","file_date_updated":"2025-12-29T14:13:01Z","OA_place":"publisher","date_published":"2025-09-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"date_updated":"2025-12-29T14:13:43Z","title":"Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance","isi":1,"issue":"9","pmid":1,"day":"01","language":[{"iso":"eng"}],"_id":"19507","status":"public","department":[{"_id":"EdHa"}],"year":"2025","OA_type":"hybrid","citation":{"ieee":"M. S. Andersen, S. Ulyanchenko, P. J. Schweiger, E. B. Hannezo, B. D. Simons, and K. B. Jensen, “Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance,” <i>Journal of Investigative Dermatology</i>, vol. 145, no. 9. Elsevier, p. 2191–2202.e5, 2025.","chicago":"Andersen, Marianne S., Svetlana Ulyanchenko, Pawel J. Schweiger, Edouard B Hannezo, Benjamin D. Simons, and Kim B. Jensen. “Spatiotemporal Switches in Progenitor Cell Fate Govern Upper Hair Follicle Growth and Maintenance.” <i>Journal of Investigative Dermatology</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">https://doi.org/10.1016/j.jid.2025.01.034</a>.","ista":"Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB. 2025. Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. Journal of Investigative Dermatology. 145(9), 2191–2202.e5.","short":"M.S. Andersen, S. Ulyanchenko, P.J. Schweiger, E.B. Hannezo, B.D. Simons, K.B. Jensen, Journal of Investigative Dermatology 145 (2025) 2191–2202.e5.","apa":"Andersen, M. S., Ulyanchenko, S., Schweiger, P. J., Hannezo, E. B., Simons, B. D., &#38; Jensen, K. B. (2025). Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. <i>Journal of Investigative Dermatology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">https://doi.org/10.1016/j.jid.2025.01.034</a>","ama":"Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB. Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth and maintenance. <i>Journal of Investigative Dermatology</i>. 2025;145(9):2191-2202.e5. doi:<a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">10.1016/j.jid.2025.01.034</a>","mla":"Andersen, Marianne S., et al. “Spatiotemporal Switches in Progenitor Cell Fate Govern Upper Hair Follicle Growth and Maintenance.” <i>Journal of Investigative Dermatology</i>, vol. 145, no. 9, Elsevier, 2025, p. 2191–2202.e5, doi:<a href=\"https://doi.org/10.1016/j.jid.2025.01.034\">10.1016/j.jid.2025.01.034</a>."},"date_created":"2025-04-06T22:01:32Z","month":"09","external_id":{"isi":["001604396400001"],"pmid":["40010488"]},"author":[{"first_name":"Marianne S.","last_name":"Andersen","full_name":"Andersen, Marianne S."},{"full_name":"Ulyanchenko, Svetlana","first_name":"Svetlana","last_name":"Ulyanchenko"},{"full_name":"Schweiger, Pawel J.","last_name":"Schweiger","first_name":"Pawel J."},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"},{"full_name":"Simons, Benjamin D.","first_name":"Benjamin D.","last_name":"Simons"},{"full_name":"Jensen, Kim B.","first_name":"Kim B.","last_name":"Jensen"}],"has_accepted_license":"1","oa":1},{"article_processing_charge":"Yes","quality_controlled":"1","article_type":"original","abstract":[{"text":"Introduction: Acid-growth theory has been postulated in the 70s to explain the rapid elongation of plant cells in response to the hormone auxin. More recently, it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting acidification of the apoplast is the principal mechanism by which auxin and other hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances, the impact of this acidification on the mechanical properties of the cell wall remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate hormone-induced tissue elongation and local changes in cell wall mechanical properties. Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase (PME), along with calcium chelators, we investigate the effect of pectin modification in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification of apoplast is necessary and sufficient to induce cell elongation through promoting cell wall softening. Moreover, we show that enhanced PME activity can induce both cell wall softening or stiffening in extracellular calcium dependent-manner and that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion: Our results confirm a dual role of PME in plant cell elongation. However, further investigation is needed to assess the status of pectin following short- or long-term PME treatments in order to determine if pectin methyl-esterification might promote its degradation as well as the role of PME inhibitors upon PME induction.","lang":"eng"}],"publication":"Frontiers in Plant Science","project":[{"name":"Hormonal cross-talk in plant organogenesis","grant_number":"207362","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854","name":"IST Austria Open Access Fund"}],"file":[{"date_updated":"2025-07-31T07:28:54Z","date_created":"2025-07-31T07:28:54Z","content_type":"application/pdf","access_level":"open_access","creator":"dernst","file_size":3665187,"checksum":"9e6b8b53ba56d4a24a9bd91cf6d2dc58","file_name":"2025_FrontiersPlantSc_Gallemi.pdf","file_id":"20093","success":1,"relation":"main_file"}],"acknowledgement":"The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127). We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler for the efforts to establish immunodetection method.","doi":"10.3389/fpls.2025.1612366","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"}],"volume":16,"article_number":"1612366","oa_version":"Published Version","publication_status":"published","corr_author":"1","scopus_import":"1","publisher":"Frontiers Media","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"DOAJ_listed":"1","type":"journal_article","fulldoi":"https://doi.org/10.3389/fpls.2025.1612366","ec_funded":1,"month":"07","external_id":{"isi":["001530690900001"],"pmid":["40688689"]},"author":[{"orcid":"0000-0003-4675-6893","full_name":"Gallemi, Marçal","first_name":"Marçal","last_name":"Gallemi","id":"460C6802-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","last_name":"Montesinos López","first_name":"Juan C"},{"first_name":"Nikola","id":"18e95355-e05a-11ea-a9c0-8fba1b89e83a","last_name":"Zarevski","full_name":"Zarevski, Nikola"},{"last_name":"Pribyl","first_name":"Jan","full_name":"Pribyl, Jan"},{"last_name":"Skládal","first_name":"Petr","full_name":"Skládal, Petr"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva"}],"has_accepted_license":"1","oa":1,"APC_amount":"3642,79 EUR","status":"public","department":[{"_id":"EdHa"},{"_id":"EvBe"},{"_id":"CaGu"}],"year":"2025","OA_type":"gold","citation":{"ieee":"M. Gallemi <i>et al.</i>, “Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties,” <i>Frontiers in Plant Science</i>, vol. 16. Frontiers Media, 2025.","chicago":"Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl, Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2025. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>.","ista":"Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB, Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.","short":"M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B. Hannezo, E. Benková, Frontiers in Plant Science 16 (2025).","apa":"Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P., Hannezo, E. B., &#38; Benková, E. (2025). Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2025.1612366\">https://doi.org/10.3389/fpls.2025.1612366</a>","ama":"Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties. <i>Frontiers in Plant Science</i>. 2025;16. doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>","mla":"Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.” <i>Frontiers in Plant Science</i>, vol. 16, 1612366, Frontiers Media, 2025, doi:<a href=\"https://doi.org/10.3389/fpls.2025.1612366\">10.3389/fpls.2025.1612366</a>."},"date_created":"2025-07-27T22:01:26Z","title":"Dual role of pectin methyl esterase activity in the regulation of plant cell wall biophysical properties","pmid":1,"isi":1,"PlanS_conform":"1","day":"04","language":[{"iso":"eng"}],"_id":"20080","intvolume":"        16","publication_identifier":{"eissn":["1664-462X"]},"file_date_updated":"2025-07-31T07:28:54Z","OA_place":"publisher","date_published":"2025-07-04T00:00:00Z","ddc":["580"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-05-20T07:53:03Z"},{"APC_amount":"5766,07 EUR","status":"public","department":[{"_id":"EdHa"},{"_id":"MiSi"}],"citation":{"mla":"Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34, e2504064122, National Academy of Sciences, 2025, doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>.","apa":"Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., &#38; Hannezo, E. B. (2025). Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>","short":"M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of the National Academy of Sciences 122 (2025).","ama":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis of mixed cell populations. <i>Proceedings of the National Academy of Sciences</i>. 2025;122(34). doi:<a href=\"https://doi.org/10.1073/pnas.2504064122\">10.1073/pnas.2504064122</a>","ieee":"M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated chemotaxis of mixed cell populations,” <i>Proceedings of the National Academy of Sciences</i>, vol. 122, no. 34. National Academy of Sciences, 2025.","ista":"Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34), e2504064122.","chicago":"Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2025. <a href=\"https://doi.org/10.1073/pnas.2504064122\">https://doi.org/10.1073/pnas.2504064122</a>."},"date_created":"2025-09-07T22:01:32Z","year":"2025","OA_type":"hybrid","month":"08","external_id":{"pmid":["40838890"],"isi":["001562181600001"]},"author":[{"id":"50B2A802-6007-11E9-A42B-EB23E6697425","last_name":"Ucar","first_name":"Mehmet C","orcid":"0000-0003-0506-4217","full_name":"Ucar, Mehmet C"},{"last_name":"Zane","id":"60f7509a-f652-11ea-9d86-b963d6490d7c","first_name":"Alsberga","orcid":"0009-0003-0415-7603","full_name":"Zane, Alsberga"},{"orcid":"0000-0002-7698-3061","full_name":"Alanko, Jonna H","first_name":"Jonna H","last_name":"Alanko","id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"has_accepted_license":"1","oa":1,"file_date_updated":"2025-09-08T07:23:29Z","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"intvolume":"       122","OA_place":"publisher","ddc":["570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2025-08-26T00:00:00Z","date_updated":"2026-05-20T08:59:54Z","title":"Self-generated chemotaxis of mixed cell populations","day":"26","PlanS_conform":"1","isi":1,"pmid":1,"issue":"34","_id":"20289","language":[{"iso":"eng"}],"file":[{"date_updated":"2025-09-08T07:23:29Z","date_created":"2025-09-08T07:23:29Z","content_type":"application/pdf","access_level":"open_access","file_name":"2025_PNAS_Ucar.pdf","file_size":16069140,"creator":"dernst","checksum":"b36abd92673b6d76376fc9434bad52cc","file_id":"20307","success":1,"relation":"main_file"}],"acknowledgement":"We thank all members of the M.S. and E.H. groups for stimulating discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support facility of the Institute of Science and Technology Austria for their excellent support and provided resources for the experimental research. In particular, we thank Jack Merrin from the Nanofabrication facility who generated the microfabricated channel used in this study. This work received funding fromt he European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield Strategic Research Fellowship in the Physics of Life and Quantitative Biology.","related_material":{"link":[{"url":"https://github.com/mehmetcanucar/Self-generated-chemotaxis","relation":"software"}]},"doi":"10.1073/pnas.2504064122","article_processing_charge":"Yes (in subscription journal)","quality_controlled":"1","article_type":"original","abstract":[{"lang":"eng","text":"Cell and tissue movement in development, cancer invasion, and immune response relies on chemical or mechanical guidance cues. In many systems, this behavior is locally directed by self-generated signaling gradients rather than long-range, prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally and navigate through self-generated gradients remains largely unexplored. Here, we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous cell populations. We find that the relative chemotactic sensitivities of different cell populations control their long-time coupling and comigration dynamics, with boundary conditions such as external cell and attractant reservoirs substantially influencing the migration patterns. Our model predicts an optimal parameter regime that enables robust and colocalized migration. We test our theoretical predictions with in vitro experiments demonstrating the comigration of distinct immune cell populations, and quantitatively reproduce observed migration patterns under wild-type and perturbed conditions. Interestingly, immune cell comigration occurs close to the predicted optimal regime. Finally, we incorporate mechanical interactions into our framework, revealing a nontrivial interplay between chemotactic and mechanical nonreciprocity in driving collective migration. Together, our findings suggest that self-generated chemotaxis is a robust strategy for the navigation of mixed cell populations."}],"project":[{"_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288","call_identifier":"H2020"}],"publication":"Proceedings of the National Academy of Sciences","scopus_import":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"National Academy of Sciences","fulldoi":"https://doi.org/10.1073/pnas.2504064122","ec_funded":1,"type":"journal_article","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"NanoFab"}],"article_number":"e2504064122","volume":122,"publication_status":"published","oa_version":"Published Version","corr_author":"1"},{"scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"publisher":"Springer Nature","ec_funded":1,"fulldoi":"https://doi.org/10.1038/s41467-025-62882-9","type":"journal_article","DOAJ_listed":"1","acknowledged_ssus":[{"_id":"Bio"}],"article_number":"8440","volume":16,"publication_status":"published","oa_version":"Published Version","corr_author":"1","file":[{"date_created":"2025-10-13T12:37:04Z","date_updated":"2025-10-13T12:37:04Z","content_type":"application/pdf","access_level":"open_access","creator":"dernst","file_size":2816813,"checksum":"d1656576883b23902545328e2d640234","file_name":"2025_NatureComm_Sahu.pdf","success":1,"file_id":"20464","relation":"main_file"}],"acknowledgement":"We thank Alois Schlögl, Paula Sanematsu, Susana Moreno Flores, Bernat Corominas-Murtra, Stefania Tavano, Gayathri Singharaju, and Hannezo group members for helpful discussions, the Bioimaging facility at ISTA, as well as Matthias Merkel and Lisa Manning for sharing the 3D Voronoi code. We also thank the Champalimaud animal facility, Anna Pezzarossa and the Champalimaud ABBE platform for the help with microscopy and image processing. This work was supported by EMBO (ALTF 522-2021), a Fundação para a Ciência e Tecnologia grant to A.S.D. (PTDC/MED-ONC/5553/2020), as well as the European Research Council (grant 851288 to EH). A.S.D., S.C., and R.M.S. are supported by QuantOCancer Project Horizon European Union’s Horizon 2020 program (grant agreement No 810653).","doi":"10.1038/s41467-025-62882-9","article_processing_charge":"Yes","quality_controlled":"1","article_type":"original","abstract":[{"lang":"eng","text":"Homeostasis relies on a precise balance of fate choices between renewal and differentiation. Although progress has been done to characterize the dynamics of single-cell fate choices, their underlying mechanistic basis often remains unclear. Concentrating on skin epidermis as a paradigm for multilayered tissues with complex fate choices, we develop a 3D vertex-based model with proliferation in the basal layer, showing that mechanical competition for space naturally gives rise to homeostasis and neutral drift dynamics that are seen experimentally. We then explore the effect of introducing mechanical heterogeneities between cellular subpopulations. We uncover that relatively small tension heterogeneities, reflected by distinct morphological changes in single-cell shapes, can be sufficient to heavily tilt cellular dynamics towards exponential growth. We thus derive a master relationship between cell shape and long-term clonal dynamics, which we validated during basal cell carcinoma initiation in mouse epidermis. Altogether, we propose a theoretical framework to link mechanical forces, quantitative cellular morphologies and cellular fate outcomes in complex tissues."}],"publication":"Nature Communications","project":[{"_id":"628f3fb1-2b32-11ec-9570-83ce778803f7","grant_number":"ALTF 522-2021","name":"Biomechanics of stem cell fate determination"},{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"file_date_updated":"2025-10-13T12:37:04Z","intvolume":"        16","publication_identifier":{"eissn":["2041-1723"]},"OA_place":"publisher","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"date_published":"2025-09-26T00:00:00Z","date_updated":"2026-05-20T08:52:01Z","title":"Mechanical control of cell fate decisions in the skin epidermis","day":"26","pmid":1,"isi":1,"language":[{"iso":"eng"}],"_id":"20424","APC_amount":"7068 EUR","status":"public","department":[{"_id":"EdHa"}],"date_created":"2025-10-05T22:01:34Z","citation":{"chicago":"Sahu, Preeti, Sara Monteiro-Ferreira, Sara Canato, Raquel Maia Soares, Adriana Sánchez-Danés, and Edouard B Hannezo. “Mechanical Control of Cell Fate Decisions in the Skin Epidermis.” <i>Nature Communications</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41467-025-62882-9\">https://doi.org/10.1038/s41467-025-62882-9</a>.","ista":"Sahu P, Monteiro-Ferreira S, Canato S, Soares RM, Sánchez-Danés A, Hannezo EB. 2025. Mechanical control of cell fate decisions in the skin epidermis. Nature Communications. 16, 8440.","ieee":"P. Sahu, S. Monteiro-Ferreira, S. Canato, R. M. Soares, A. Sánchez-Danés, and E. B. Hannezo, “Mechanical control of cell fate decisions in the skin epidermis,” <i>Nature Communications</i>, vol. 16. Springer Nature, 2025.","ama":"Sahu P, Monteiro-Ferreira S, Canato S, Soares RM, Sánchez-Danés A, Hannezo EB. Mechanical control of cell fate decisions in the skin epidermis. <i>Nature Communications</i>. 2025;16. doi:<a href=\"https://doi.org/10.1038/s41467-025-62882-9\">10.1038/s41467-025-62882-9</a>","short":"P. Sahu, S. Monteiro-Ferreira, S. Canato, R.M. Soares, A. Sánchez-Danés, E.B. Hannezo, Nature Communications 16 (2025).","apa":"Sahu, P., Monteiro-Ferreira, S., Canato, S., Soares, R. M., Sánchez-Danés, A., &#38; Hannezo, E. B. (2025). Mechanical control of cell fate decisions in the skin epidermis. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-025-62882-9\">https://doi.org/10.1038/s41467-025-62882-9</a>","mla":"Sahu, Preeti, et al. “Mechanical Control of Cell Fate Decisions in the Skin Epidermis.” <i>Nature Communications</i>, vol. 16, 8440, Springer Nature, 2025, doi:<a href=\"https://doi.org/10.1038/s41467-025-62882-9\">10.1038/s41467-025-62882-9</a>."},"OA_type":"gold","year":"2025","month":"09","external_id":{"isi":["001582555200011"],"pmid":["41006218"]},"author":[{"full_name":"Sahu, Preeti","id":"55BA52EE-A185-11EA-88FD-18AD3DDC885E","last_name":"Sahu","first_name":"Preeti"},{"full_name":"Monteiro-Ferreira, Sara","last_name":"Monteiro-Ferreira","first_name":"Sara"},{"full_name":"Canato, Sara","last_name":"Canato","first_name":"Sara"},{"last_name":"Soares","first_name":"Raquel Maia","full_name":"Soares, Raquel Maia"},{"full_name":"Sánchez-Danés, Adriana","last_name":"Sánchez-Danés","first_name":"Adriana"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}],"oa":1,"has_accepted_license":"1"},{"quality_controlled":"1","article_processing_charge":"No","publication":"Nature Physics","project":[{"_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","grant_number":"ALTF 343-2022","name":"A mechano-chemical theory for stem cell fate decisions in organoid development"}],"article_type":"original","abstract":[{"text":"Haptotaxis is the process of directed cell migration along gradients of extracellular matrix density and is central to morphogenesis, immune responses and cancer invasion. It is commonly assumed that cells respond to these gradients by migrating directionally towards the regions of highest ligand density. In contrast with this view, here we show that cells exposed to micropatterned fibronectin gradients exhibit a wide range of complex trajectories, including directed haptotactic migration up the gradient but also linear oscillations and circles with extended periods of migration down the gradient. To explain this behaviour, we developed a biophysical model of haptotactic cell migration based on a coarse-grained molecular clutch model coupled to persistent stochastic polarity dynamics. Although initial haptotactic migration is explained by the differential friction at the front and back of the cell, the observed complex trajectories over longer timescales arise from the interplay between differential friction, persistence and physical confinement. Overall, our study reveals that confinement and persistence modulate the ability of cells to sense and respond to haptotactic cues and provides a framework for understanding how cells navigate complex environments.","lang":"eng"}],"doi":"10.1038/s41567-025-03015-3","acknowledgement":"We thank all the members of our groups for discussions and support. We thank A. Menéndez, S. Usieto, M. Purciolas and E. Coderch for technical assistance. We thank G. Charras (London Centre for Nanotechnology, UK) and M. Sheetz (Columbia University, USA) for sharing cells used in this work. We thank J. Ivaska (University of Turku, Finland) for sharing integrin α5-GFP DNA plasmid. We thank P. Guillamat for technical advice and A. Labernardie for providing the microfluidic channels. We thank M. Gómez-González for sharing the 2D traction microscopy algorithm. Finally, we thank P. Guillamat, J. Abenza, G. Ceada, L. Faure, E. Dalaka, M. Matejčić, A. Beedle, I. Granero, O. Baguer, A. Albajar and N. Chahare for discussions. This paper was funded by the Generalitat de Catalunya (Grant Nos. AGAUR SGR-2017-01602 to X.T. and 2021 SGR 00523 to R.S. and the CERCA Programme and ICREA Academia awards to P.R.-C.), the Spanish Ministry for Science and Innovation MICCINN/FEDER (Grant Nos. PID2021-128635NB-I00, MCIN/AEI/10.13039/501100011033 and ERDF-EU A way of making Europe to X.T., PID2021-128674OB-I00 and CNS2022-135533 to R.S. and PID2019-110298GB-I00 to P.R.-C.), the European Research Council (Grant Nos. 101097753 to P.R.-C. and Adv-883739 to X.T.), Fundació la Marató de TV3 (Project Award 201903-30-31-32 to X.T.), the European Commission (Grant No. H2020-FETPROACT-01-2016-731957 to P.R.-C. and X.T.) and La Caixa Foundation (Grant No. LCF/PR/HR20/52400004 to P.R.-C. and X.T.). R.S. is a Serra-Hunter fellow. D.B.B. was supported by the NOMIS foundation as a NOMIS fellow, by the European Molecular Biology Organization (Postdoctoral Fellowship ALTF 343-2022) and by the Austrian Academy of Sciences through an APART-MINT Fellowship. I.C.F. acknowledges support from the European Foundation for the Study of Chronic Liver Failure. IBEC is recipient of a Severo Ochoa Award of Excellence from MINECO.","page":"1638-1647","volume":21,"corr_author":"1","publication_status":"published","oa_version":"Preprint","publisher":"Springer Nature","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2024.12.02.626413"}],"scopus_import":"1","fulldoi":"https://doi.org/10.1038/s41567-025-03015-3","type":"journal_article","external_id":{"isi":["001581659900001"]},"month":"10","oa":1,"author":[{"full_name":"Fortunato, Isabela Corina","last_name":"Fortunato","first_name":"Isabela Corina"},{"id":"e1e86031-6537-11eb-953a-f7ab92be508d","last_name":"Brückner","first_name":"David","orcid":"0000-0001-7205-2975","full_name":"Brückner, David"},{"full_name":"Grosser, Steffen","last_name":"Grosser","first_name":"Steffen"},{"last_name":"Nautiyal","first_name":"Rohit","full_name":"Nautiyal, Rohit"},{"first_name":"Leone","last_name":"Rossetti","full_name":"Rossetti, Leone"},{"full_name":"Bosch-Padrós, Miquel","first_name":"Miquel","last_name":"Bosch-Padrós"},{"full_name":"Trebicka, Jonel","last_name":"Trebicka","first_name":"Jonel"},{"first_name":"Pere","last_name":"Roca-Cusachs","full_name":"Roca-Cusachs, Pere"},{"first_name":"Raimon","last_name":"Sunyer","full_name":"Sunyer, Raimon"},{"last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Xavier","last_name":"Trepat","full_name":"Trepat, Xavier"}],"status":"public","date_created":"2025-10-05T22:01:36Z","citation":{"mla":"Fortunato, Isabela Corina, et al. “Single-Cell Migration along and against Confined Haptotactic Gradients.” <i>Nature Physics</i>, vol. 21, Springer Nature, 2025, pp. 1638–47, doi:<a href=\"https://doi.org/10.1038/s41567-025-03015-3\">10.1038/s41567-025-03015-3</a>.","ama":"Fortunato IC, Brückner D, Grosser S, et al. Single-cell migration along and against confined haptotactic gradients. <i>Nature Physics</i>. 2025;21:1638-1647. doi:<a href=\"https://doi.org/10.1038/s41567-025-03015-3\">10.1038/s41567-025-03015-3</a>","apa":"Fortunato, I. C., Brückner, D., Grosser, S., Nautiyal, R., Rossetti, L., Bosch-Padrós, M., … Trepat, X. (2025). Single-cell migration along and against confined haptotactic gradients. <i>Nature Physics</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41567-025-03015-3\">https://doi.org/10.1038/s41567-025-03015-3</a>","short":"I.C. Fortunato, D. Brückner, S. Grosser, R. Nautiyal, L. Rossetti, M. Bosch-Padrós, J. Trebicka, P. Roca-Cusachs, R. Sunyer, E.B. Hannezo, X. Trepat, Nature Physics 21 (2025) 1638–1647.","chicago":"Fortunato, Isabela Corina, David Brückner, Steffen Grosser, Rohit Nautiyal, Leone Rossetti, Miquel Bosch-Padrós, Jonel Trebicka, et al. “Single-Cell Migration along and against Confined Haptotactic Gradients.” <i>Nature Physics</i>. Springer Nature, 2025. <a href=\"https://doi.org/10.1038/s41567-025-03015-3\">https://doi.org/10.1038/s41567-025-03015-3</a>.","ista":"Fortunato IC, Brückner D, Grosser S, Nautiyal R, Rossetti L, Bosch-Padrós M, Trebicka J, Roca-Cusachs P, Sunyer R, Hannezo EB, Trepat X. 2025. Single-cell migration along and against confined haptotactic gradients. Nature Physics. 21, 1638–1647.","ieee":"I. C. Fortunato <i>et al.</i>, “Single-cell migration along and against confined haptotactic gradients,” <i>Nature Physics</i>, vol. 21. Springer Nature, pp. 1638–1647, 2025."},"OA_type":"green","year":"2025","department":[{"_id":"EdHa"}],"title":"Single-cell migration along and against confined haptotactic gradients","_id":"20431","language":[{"iso":"eng"}],"day":"01","isi":1,"OA_place":"repository","intvolume":"        21","publication_identifier":{"eissn":["1745-2481"],"issn":["1745-2473"]},"date_published":"2025-10-01T00:00:00Z","date_updated":"2026-01-05T14:26:28Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2025-10-31T00:00:00Z","date_updated":"2026-05-20T08:58:06Z","ddc":["530"],"intvolume":"        15","publication_identifier":{"eissn":["2160-3308"]},"file_date_updated":"2025-12-01T07:30:00Z","OA_place":"publisher","issue":"4","PlanS_conform":"1","day":"31","_id":"20708","language":[{"iso":"eng"}],"arxiv":1,"title":"Emergent dynamics of active elastic microbeams","department":[{"_id":"EdHa"},{"_id":"JePa"}],"year":"2025","OA_type":"gold","citation":{"chicago":"Martinet, Quentin, Yuting I Li, A. Aubret, Edouard B Hannezo, and Jérémie A Palacci. “Emergent Dynamics of Active Elastic Microbeams.” <i>Physical Review X</i>. American Physical Society, 2025. <a href=\"https://doi.org/10.1103/rjk2-q2wh\">https://doi.org/10.1103/rjk2-q2wh</a>.","ista":"Martinet Q, Li YI, Aubret A, Hannezo EB, Palacci JA. 2025. Emergent dynamics of active elastic microbeams. Physical Review X. 15(4), 041017.","ieee":"Q. Martinet, Y. I. Li, A. Aubret, E. B. Hannezo, and J. A. Palacci, “Emergent dynamics of active elastic microbeams,” <i>Physical Review X</i>, vol. 15, no. 4. American Physical Society, 2025.","mla":"Martinet, Quentin, et al. “Emergent Dynamics of Active Elastic Microbeams.” <i>Physical Review X</i>, vol. 15, no. 4, 041017, American Physical Society, 2025, doi:<a href=\"https://doi.org/10.1103/rjk2-q2wh\">10.1103/rjk2-q2wh</a>.","ama":"Martinet Q, Li YI, Aubret A, Hannezo EB, Palacci JA. Emergent dynamics of active elastic microbeams. <i>Physical Review X</i>. 2025;15(4). doi:<a href=\"https://doi.org/10.1103/rjk2-q2wh\">10.1103/rjk2-q2wh</a>","apa":"Martinet, Q., Li, Y. I., Aubret, A., Hannezo, E. B., &#38; Palacci, J. A. (2025). Emergent dynamics of active elastic microbeams. <i>Physical Review X</i>. American Physical Society. <a href=\"https://doi.org/10.1103/rjk2-q2wh\">https://doi.org/10.1103/rjk2-q2wh</a>","short":"Q. Martinet, Y.I. Li, A. Aubret, E.B. Hannezo, J.A. Palacci, Physical Review X 15 (2025)."},"date_created":"2025-11-30T23:02:08Z","APC_amount":"4695,11 EUR","status":"public","author":[{"full_name":"Martinet, Quentin","orcid":"0000-0002-2916-6632","first_name":"Quentin","id":"b37485a8-d343-11eb-a0e9-df8c484ef8ab","last_name":"Martinet"},{"full_name":"Li, Yuting I","last_name":"Li","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","first_name":"Yuting I"},{"full_name":"Aubret, A.","last_name":"Aubret","first_name":"A."},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"last_name":"Palacci","id":"8fb92548-2b22-11eb-b7c1-a3f0d08d7c7d","first_name":"Jérémie A","orcid":"0000-0002-7253-9465","full_name":"Palacci, Jérémie A"}],"has_accepted_license":"1","oa":1,"month":"10","external_id":{"arxiv":["2508.20642"]},"DOAJ_listed":"1","type":"journal_article","fulldoi":"https://doi.org/10.1103/rjk2-q2wh","ec_funded":1,"scopus_import":"1","publisher":"American Physical Society","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa_version":"Published Version","publication_status":"published","corr_author":"1","volume":15,"article_number":"041017","acknowledgement":"The authors thank Andela Saric, Christoph Zechner, and Paul Robin for helpful discussions. J. P. acknowledges support by ERC grant (VULCAN, 101086998) and U.S. ARO under Award No. W911NF2310008. Y. I. L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 101034413.","doi":"10.1103/rjk2-q2wh","file":[{"file_id":"20714","success":1,"file_name":"2025_PhysicalReviewX_Martinet.pdf","checksum":"bb64ea9f2c400205fd89e9bdd15cc850","creator":"dernst","file_size":5902259,"relation":"main_file","date_updated":"2025-12-01T07:30:00Z","date_created":"2025-12-01T07:30:00Z","access_level":"open_access","content_type":"application/pdf"}],"abstract":[{"text":"In equilibrium, the physical properties of matter are set by the interactions between the constituents. In contrast, the energy input of the individual components controls the behavior of synthetic or living active matter. Great progress has been made in understanding the emergent phenomena in active fluids, though their inability to resist shear forces hinders their practical use. This motivates the exploration of active solids as shape-shifting materials, yet, we lack controlled synthetic systems to devise active solids with unconventional properties. Here we build active elastic beams from dozens of active colloids and unveil complex emergent behaviors such as self-oscillations or persistent rotations. Developing tensile tests at the microscale, we show that the active beams are ultrasoft materials, with large (nonequilibrium) fluctuations. Combining experiments, theory, and stochastic inference, we show that the dynamics of the active beams can be mapped on different phase transitions which are tuned by boundary conditions. More quantitatively, we assess all relevant parameters by independent measurements or first-principles calculations, and find that our theoretical description agrees with the experimental observations. Our results demonstrate that the simple addition of activity to an elastic beam unveils novel physics and can inspire design strategies for active solids and functional microscopic machines.","lang":"eng"}],"article_type":"original","project":[{"name":"VULCAN: matter, powered from within","grant_number":"101086998","_id":"bdac72da-d553-11ed-ba76-eae56e802b74"},{"call_identifier":"H2020","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","grant_number":"101034413","name":"IST-BRIDGE: International postdoctoral program"}],"publication":"Physical Review X","article_processing_charge":"Yes","quality_controlled":"1"},{"year":"2025","doi":"10.1101/2025.02.14.638262","date_created":"2025-10-14T07:25:27Z","citation":{"short":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J. Heisenberg, BioRxiv (n.d.).","apa":"Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &#38; Heisenberg, C.-P. J. (n.d.). Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>","ama":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>","mla":"Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>.","ieee":"S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P. J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties,” <i>bioRxiv</i>. .","chicago":"Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo, Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading by Balancing Spreading Forces with Tissue Material Properties.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.02.14.638262\">https://doi.org/10.1101/2025.02.14.638262</a>.","ista":"Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ. Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties. bioRxiv, <a href=\"https://doi.org/10.1101/2025.02.14.638262\">10.1101/2025.02.14.638262</a>."},"related_material":{"record":[{"id":"20441","relation":"dissertation_contains","status":"public"},{"relation":"later_version","status":"public","id":"22608"}]},"department":[{"_id":"CaHe"},{"_id":"EdHa"}],"status":"public","oa":1,"publication":"bioRxiv","author":[{"orcid":"0000-0001-8421-5508","full_name":"Naik, Suyash","last_name":"Naik","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87","first_name":"Suyash"},{"full_name":"Keta, Yann-Edwin","last_name":"Keta","first_name":"Yann-Edwin"},{"full_name":"Pranjic-Ferscha, Kornelija","first_name":"Kornelija","last_name":"Pranjic-Ferscha","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Henkes","first_name":"Silke","full_name":"Henkes, Silke"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"abstract":[{"lang":"eng","text":"For tissues to spread, they must be deformable while maintaining their structural integrity. How these opposing requirements are balanced within spreading tissues is not yet well understood. Here, we show that keratin intermediate filaments function in epithelial spreading by adapting tissue mechanical resilience to the stresses arising in the tissue during the spreading process. By analysing the expansion of the enveloping cell layer (EVL) over the large yolk cell in early zebrafish embryos in vivo, we found that keratin network maturation in EVL cells is promoted by stresses building up within the spreading tissue. Through genetic interference and tissue rheology experiments, complemented by a vertex model with mechanochemical feedback, we demonstrate that stress-induced keratin network maturation in the EVL increases tissue viscosity, which is essential for preventing tissue rupture. Interestingly, keratins are also required in the yolk cell for mechanosensitive actomyosin network contraction and flow, the force-generating processes pulling the EVL. These dual mechanosensitive functions of keratins enable a balance between pulling force production in the yolk cell and the mechanical resilience of the EVL against stresses generated by these pulling forces, thereby ensuring uniform and robust tissue spreading."}],"month":"02","article_processing_charge":"No","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-07-29T10:33:31Z","date_published":"2025-02-17T00:00:00Z","license":"https://creativecommons.org/licenses/by-nd/4.0/","type":"preprint","fulldoi":"https://doi.org/10.1101/2025.02.14.638262","OA_place":"repository","das_tickbox":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nd/4.0/legalcode","image":"/image/cc_by_nd.png","name":"Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0)","short":"CC BY-ND (4.0)"},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2025.02.14.638262"}],"language":[{"iso":"eng"}],"_id":"20465","corr_author":"1","oa_version":"Preprint","publication_status":"draft","day":"17","title":"Keratins coordinate tissue spreading by balancing spreading forces with tissue material properties"},{"publisher":"Cold Spring Harbor Laboratory Press","scopus_import":"1","type":"journal_article","ec_funded":1,"fulldoi":"https://doi.org/10.1101/cshperspect.a041653","volume":17,"article_number":"a041653","corr_author":"1","oa_version":"None","publication_status":"published","doi":"10.1101/cshperspect.a041653","acknowledgement":"We thank Fridtjof Brauns, Anna Kicheva, and Carl-Philipp Heisenberg for a critical reading of the manuscript and Claudia Flandoli for the artwork in the figures. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022). This work received funding from the European Research Council (ERC) under the European Union\\u2019s Horizon 2020 Research and Innovation Programme Grant Agreement no. 851288.","quality_controlled":"1","article_processing_charge":"No","researchdata_availability":"no","project":[{"_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","name":"A mechano-chemical theory for stem cell fate decisions in organoid development","grant_number":"ALTF 343-2022"},{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"publication":"Cold Spring Harbor Perspectives in Biology","article_type":"original","abstract":[{"lang":"eng","text":"The importance of physical forces in the morphogenesis, homeostatic function, and pathological dysfunction of multicellular tissues is being increasingly characterized, both theoretically and experimentally. Analogies between biological systems and inert materials such as foams, gels, and liquid crystals have provided striking insights into the core design principles underlying multicellular organization. However, these connections can seem surprising given that a key feature of multicellular systems is their ability to constantly consume energy, providing an active origin for the forces that they produce. Key emerging questions are, therefore, to understand whether and how this activity grants tissues novel properties that do not have counterparts in classical materials, as well as their consequences for biological function. Here, we review recent discoveries at the intersection of active matter and tissue biology, with an emphasis on how modeling and experiments can be combined to understand the dynamics of multicellular systems. These approaches suggest that a number of key biological tissue-scale phenomena, such as morphogenetic shape changes, collective migration, or fate decisions, share unifying design principles that can be described by physical models of tissue active matter."}],"das_tickbox":"0","publication_identifier":{"issn":["1943-0264"]},"supplementarymaterial":"no","intvolume":"        17","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_published":"2025-04-01T00:00:00Z","date_updated":"2026-10-01T11:03:02Z","title":"Tissue active matter: Integrating mechanics and signaling into dynamical models","_id":"18960","language":[{"iso":"eng"}],"isi":1,"pmid":1,"issue":"4","day":"01","status":"public","year":"2025","OA_type":"closed access","citation":{"ieee":"D. Brückner and E. B. Hannezo, “Tissue active matter: Integrating mechanics and signaling into dynamical models,” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 17, no. 4. Cold Spring Harbor Laboratory Press, 2025.","ista":"Brückner D, Hannezo EB. 2025. Tissue active matter: Integrating mechanics and signaling into dynamical models. Cold Spring Harbor Perspectives in Biology. 17(4), a041653.","chicago":"Brückner, David, and Edouard B Hannezo. “Tissue Active Matter: Integrating Mechanics and Signaling into Dynamical Models.” <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press, 2025. <a href=\"https://doi.org/10.1101/cshperspect.a041653\">https://doi.org/10.1101/cshperspect.a041653</a>.","short":"D. Brückner, E.B. Hannezo, Cold Spring Harbor Perspectives in Biology 17 (2025).","apa":"Brückner, D., &#38; Hannezo, E. B. (2025). Tissue active matter: Integrating mechanics and signaling into dynamical models. <i>Cold Spring Harbor Perspectives in Biology</i>. Cold Spring Harbor Laboratory Press. <a href=\"https://doi.org/10.1101/cshperspect.a041653\">https://doi.org/10.1101/cshperspect.a041653</a>","ama":"Brückner D, Hannezo EB. Tissue active matter: Integrating mechanics and signaling into dynamical models. <i>Cold Spring Harbor Perspectives in Biology</i>. 2025;17(4). doi:<a href=\"https://doi.org/10.1101/cshperspect.a041653\">10.1101/cshperspect.a041653</a>","mla":"Brückner, David, and Edouard B. Hannezo. “Tissue Active Matter: Integrating Mechanics and Signaling into Dynamical Models.” <i>Cold Spring Harbor Perspectives in Biology</i>, vol. 17, no. 4, a041653, Cold Spring Harbor Laboratory Press, 2025, doi:<a href=\"https://doi.org/10.1101/cshperspect.a041653\">10.1101/cshperspect.a041653</a>."},"date_created":"2025-01-29T13:33:47Z","department":[{"_id":"EdHa"}],"external_id":{"isi":["001456660400001"],"pmid":["38951023"]},"month":"04","author":[{"id":"e1e86031-6537-11eb-953a-f7ab92be508d","last_name":"Brückner","first_name":"David","orcid":"0000-0001-7205-2975","full_name":"Brückner, David"},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"}]},{"type":"preprint","ec_funded":1,"fulldoi":"https://doi.org/10.1101/2025.10.15.682552","biorxivid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"main_file_link":[{"url":"https://doi.org/10.1101/2025.10.15.682552","open_access":"1"}],"corr_author":"1","oa_version":"Preprint","publication_status":"draft","doi":"10.1101/2025.10.15.682552","related_material":{"record":[{"id":"22804","relation":"dissertation_contains","status":"public"}]},"acknowledgement":"We are grateful to the members of the Hannezo and Heisenberg groups for discussions and technical advice. We also thank the Imaging and Optics Facility and the Lab Support Facility at ISTA for their continuous support. Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skodowska-Curie Grant Agreement No. 101034413. The research was supported by funding to C.-P.H. from the NOMIS Foundation (Project ID 1.844) and to E.H. from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 851288).","project":[{"name":"IST-BRIDGE: International postdoctoral program","grant_number":"101034413","_id":"fc2ed2f7-9c52-11eb-aca3-c01059dda49c","call_identifier":"H2020"},{"call_identifier":"H2020","grant_number":"851288","name":"Design Principles of Branching Morphogenesis","_id":"05943252-7A3F-11EA-A408-12923DDC885E"},{"name":"NOMIS Fellowship Program","_id":"9B861AAC-BA93-11EA-9121-9846C619BF3A"}],"publication":"bioRxiv","abstract":[{"text":"Cleavage - the series of rapid cell divisions that follow fertilization - marks the onset of metazoan development and represents a deeply conserved evolutionary process. Across animals, two principal modes exist: complete (holoblastic) and incomplete (meroblastic) cleavage. While holoblastic cleavage resembles conventional cytokinesis both in vitro and in vivo, the mechanisms underlying meroblastic cleavage have remained poorly understood. Using zebrafish embryos as a model, we show that meroblastic cleavage proceeds through a distinct two-step mechanism. The process begins with the assembly and contraction of a large, arc-shaped actomyosin cable. However, this contractile event alone is insufficient to complete division. A second phase, driven by cadherin-mediated membrane adhesion, is required to invaginate the furrow ridge. Strikingly, this transition depends on mechanical uncoupling of the contractile cable from the surrounding cortex. We demonstrate that such uncoupling arises from an active nematic instability, which both enhances contractility along the cable and generates actin depletion zones that relieve lateral connections. Together, these findings reveal that meroblastic cleavage is governed not by a single actomyosin-based event but by a sequential interplay between cytoskeletal contraction and cadherin-dependent adhesion, highlighting a mechanism fundamentally distinct from canonical cytokinesis.","lang":"eng"}],"article_processing_charge":"No","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","date_updated":"2026-10-05T08:56:09Z","date_published":"2025-10-15T00:00:00Z","OA_place":"repository","_id":"22807","language":[{"iso":"eng"}],"day":"15","title":"Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination","OA_type":"green","year":"2025","date_created":"2026-09-04T12:32:21Z","citation":{"ieee":"X. Tong, Y. I. Li, J. Schelle, E. B. Hannezo, and C.-P. J. Heisenberg, “Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination,” <i>bioRxiv</i>. .","chicago":"Tong, Xin, Yuting I Li, Joséphine Schelle, Edouard B Hannezo, and Carl-Philipp J Heisenberg. “Non-Canonical Cytokinesis Driven by Mechanical Uncoupling via Nematic Flows and Adhesion-Based Invagination.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.10.15.682552\">https://doi.org/10.1101/2025.10.15.682552</a>.","ista":"Tong X, Li YI, Schelle J, Hannezo EB, Heisenberg C-PJ. Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination. bioRxiv, <a href=\"https://doi.org/10.1101/2025.10.15.682552\">10.1101/2025.10.15.682552</a>.","mla":"Tong, Xin, et al. “Non-Canonical Cytokinesis Driven by Mechanical Uncoupling via Nematic Flows and Adhesion-Based Invagination.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.10.15.682552\">10.1101/2025.10.15.682552</a>.","short":"X. Tong, Y.I. Li, J. Schelle, E.B. Hannezo, C.-P.J. Heisenberg, BioRxiv (n.d.).","apa":"Tong, X., Li, Y. I., Schelle, J., Hannezo, E. B., &#38; Heisenberg, C.-P. J. (n.d.). Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.10.15.682552\">https://doi.org/10.1101/2025.10.15.682552</a>","ama":"Tong X, Li YI, Schelle J, Hannezo EB, Heisenberg C-PJ. Non-canonical cytokinesis driven by mechanical uncoupling via nematic flows and adhesion-based invagination. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.10.15.682552\">10.1101/2025.10.15.682552</a>"},"department":[{"_id":"EdHa"},{"_id":"CaHe"}],"status":"public","oa":1,"author":[{"full_name":"Tong, Xin","last_name":"Tong","id":"50F65CDC-AA30-11E9-A72B-8A12E6697425","first_name":"Xin"},{"first_name":"Yuting I","id":"ee7a5ca8-8b71-11ed-b662-b3341c05b7eb","last_name":"Li","full_name":"Li, Yuting I"},{"last_name":"Schelle","first_name":"Joséphine","full_name":"Schelle, Joséphine"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"external_id":{"biorxivid":["2025.10.15.682552"]},"month":"10"},{"ec_funded":1,"fulldoi":"https://doi.org/10.1016/j.devcel.2025.04.010","type":"journal_article","scopus_import":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Cell Press","publication_status":"published","oa_version":"Published Version","page":"2237-2247.e4","volume":60,"acknowledgement":"We thank A. Dimitracopoulos, K. Kawaguchi, J. Vidigueira, B. Baum, I. McLaren, D. St Johnston, and members of the Buckley, Scarpa, Steventon, Kawaguchi, and Xiong labs for technical assistance and constructive feedback. We thank Ryan Greenhalgh for methods developed to obtain fluidity values from AFM data. We thank Nicola Lawrence, Alex Sossick, and Sargon Gross-Thebing from the Gurdon Institute Imaging Facility for microscopy support. Funding: this work was supported by a Wellcome Trust/Royal Society Sir Henry Dale Fellowship (215439/Z/19/Z) and UKRI-EPSRC Frontier Research Grant (EP/X023761/1, originally selected as an ERC Starting Grant) to F.X.; an ERC Consolidator Grant (772426), ERC Synergy Grant 101118729 UNFOLD, and Alexander von Humboldt Professorship ( Alexander von Humboldt Foundation) to K.F.; and an ERC Starting Grant (851288) to E.H.","doi":"10.1016/j.devcel.2025.04.010","file":[{"date_updated":"2025-12-29T13:45:05Z","date_created":"2025-12-29T13:45:05Z","content_type":"application/pdf","access_level":"open_access","checksum":"1ca6f0822c1cbd430686d5e2a4f96401","file_size":12564806,"file_name":"2025_DevelopmentalCell_McLaren.pdf","creator":"dernst","file_id":"20872","success":1,"relation":"main_file"}],"article_type":"original","abstract":[{"text":"An enlarged brain underlies the complex central nervous system of vertebrates. The dramatic expansion of the brain that diverges its shape from the spinal cord follows neural tube closure during embryonic development. Here, we show that this differential deformation is encoded by a pre-pattern of tissue material properties in chicken embryos. Using magnetic droplets and atomic force microscopy, we demonstrate that the dorsal hindbrain is more fluid than the dorsal spinal cord, resulting in a thinning versus a resisting response to increasing lumen pressure, respectively. The dorsal hindbrain exhibits reduced apical actin and a disorganized laminin matrix consistent with tissue fluidization. Blocking the activity of neural-crest-associated matrix metalloproteinases inhibits hindbrain expansion. Transplanting dorsal hindbrain cells to the spinal cord can locally create an expanded brain-like morphology in some cases. Our findings raise questions in vertebrate head evolution and suggest a general role of mechanical pre-patterning in sculpting epithelial tubes.","lang":"eng"}],"publication":"Developmental Cell","project":[{"call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","grant_number":"851288"}],"article_processing_charge":"Yes (in subscription journal)","quality_controlled":"1","ddc":["570"],"date_updated":"2026-10-20T08:25:00Z","date_published":"2025-09-08T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file_date_updated":"2025-12-29T13:45:05Z","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"intvolume":"        60","OA_place":"publisher","day":"08","PlanS_conform":"1","pmid":1,"issue":"17","isi":1,"_id":"19703","language":[{"iso":"eng"}],"title":"Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord","department":[{"_id":"EdHa"}],"citation":{"ieee":"S. B. P. Mclaren <i>et al.</i>, “Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord,” <i>Developmental Cell</i>, vol. 60, no. 17. Cell Press, p. 2237–2247.e4, 2025.","chicago":"Mclaren, Susannah B.P., Shi-lei Xue, Siyuan Ding, Alexander K. Winkel, Oscar Baldwin, Shreya Dwarakacherla, Kristian Franze, Edouard B Hannezo, and Fengzhu Xiong. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” <i>Developmental Cell</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">https://doi.org/10.1016/j.devcel.2025.04.010</a>.","ista":"Mclaren SBP, Xue S, Ding S, Winkel AK, Baldwin O, Dwarakacherla S, Franze K, Hannezo EB, Xiong F. 2025. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. Developmental Cell. 60(17), 2237–2247.e4.","mla":"Mclaren, Susannah B. P., et al. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” <i>Developmental Cell</i>, vol. 60, no. 17, Cell Press, 2025, p. 2237–2247.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">10.1016/j.devcel.2025.04.010</a>.","apa":"Mclaren, S. B. P., Xue, S., Ding, S., Winkel, A. K., Baldwin, O., Dwarakacherla, S., … Xiong, F. (2025). Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">https://doi.org/10.1016/j.devcel.2025.04.010</a>","short":"S.B.P. Mclaren, S. Xue, S. Ding, A.K. Winkel, O. Baldwin, S. Dwarakacherla, K. Franze, E.B. Hannezo, F. Xiong, Developmental Cell 60 (2025) 2237–2247.e4.","ama":"Mclaren SBP, Xue S, Ding S, et al. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. <i>Developmental Cell</i>. 2025;60(17):2237-2247.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">10.1016/j.devcel.2025.04.010</a>"},"date_created":"2025-05-18T22:02:50Z","OA_type":"hybrid","year":"2025","status":"public","author":[{"first_name":"Susannah B.P.","last_name":"Mclaren","full_name":"Mclaren, Susannah B.P."},{"first_name":"Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87","last_name":"Xue","full_name":"Xue, Shi-lei"},{"last_name":"Ding","first_name":"Siyuan","full_name":"Ding, Siyuan"},{"last_name":"Winkel","first_name":"Alexander K.","full_name":"Winkel, Alexander K."},{"last_name":"Baldwin","first_name":"Oscar","full_name":"Baldwin, Oscar"},{"full_name":"Dwarakacherla, Shreya","first_name":"Shreya","last_name":"Dwarakacherla"},{"full_name":"Franze, Kristian","last_name":"Franze","first_name":"Kristian"},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Fengzhu","last_name":"Xiong","full_name":"Xiong, Fengzhu"}],"oa":1,"has_accepted_license":"1","month":"09","external_id":{"pmid":["40347948"],"isi":["001570502100005"]}},{"acknowledgement":"European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian Academy of Sciences, 26360","related_material":{"record":[{"status":"public","relation":"research_data","id":"21439"},{"id":"22963","status":"public","relation":"later_version"},{"id":"21423","status":"public","relation":"dissertation_contains"}]},"doi":"10.1101/2025.05.20.655037","abstract":[{"text":"While tumor malignancy has been extensively studied under the prism of genetic and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal interactions with the microenvironment. This raises the hitherto untested possibility that heterogeneity of the untransformed tumor stroma can actively fuel malignant progression. As biological heterogeneity is inherently difficult to control, we adopted a reductionist approach and let tumor cells invade micro-engineered environments harboring obstacles with precision-controlled geometry. We find that not only the presence of obstacles, but more surprisingly their spatial disorder, causes a drastic shift from a collective to a single-cell mode of invasion – comparable in strength to cadherin loss. Combining live-imaging and perturbation experiments with minimal biophysical modeling, we demonstrate that cell detachments result both from local geometrical constraints and a global integration of spatial disorder over time. We show that different types of microenvironments map onto different universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang (KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with quenched disorder (KPZq). Our findings highlight generic physical principles for how the mode of cancer cell invasion depends on environmental heterogeneity, with potential implications to understand tumor evolution in vivo.","lang":"eng"}],"project":[{"name":"Pushing from within: Control of cell shape, integrity and motility by cytoskeletal pushing forces","grant_number":"101071793","_id":"bd91e723-d553-11ed-ba76-fe7eeb2185fd"},{"_id":"34d75525-11ca-11ed-8bc3-89b6307fee9d","grant_number":"26360","name":"Motile active matter models of migrating cells and chiral filaments"}],"publication":"bioRxiv","article_processing_charge":"No","fulldoi":"https://doi.org/10.1101/2025.05.20.655037","type":"preprint","main_file_link":[{"url":"https://doi.org/10.1101/2025.05.20.655037","open_access":"1"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"publication_status":"draft","oa_version":"Preprint","corr_author":"1","department":[{"_id":"GradSch"},{"_id":"EdHa"},{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"AnSa"}],"citation":{"mla":"Dunajova, Zuzana, et al. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>BioRxiv</i>, doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","short":"Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B. Hannezo, BioRxiv (n.d.).","apa":"Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K., &#38; Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>bioRxiv</i>. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>","ama":"Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>","ieee":"Z. Dunajova <i>et al.</i>, “Substrate heterogeneity promotes cancer cell dissemination through interface roughening,” <i>bioRxiv</i>. .","ista":"Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate heterogeneity promotes cancer cell dissemination through interface roughening. bioRxiv, <a href=\"https://doi.org/10.1101/2025.05.20.655037\">10.1101/2025.05.20.655037</a>.","chicago":"Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai, Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer Cell Dissemination through Interface Roughening.” <i>BioRxiv</i>, n.d. <a href=\"https://doi.org/10.1101/2025.05.20.655037\">https://doi.org/10.1101/2025.05.20.655037</a>."},"date_created":"2026-03-11T08:40:06Z","year":"2025","status":"public","author":[{"first_name":"Zuzana","id":"4B39F286-F248-11E8-B48F-1D18A9856A87","last_name":"Dunajova","full_name":"Dunajova, Zuzana"},{"first_name":"Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","last_name":"Tasciyan","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X"},{"first_name":"Juraj","last_name":"Majek","id":"3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1","full_name":"Majek, Juraj"},{"last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"full_name":"Sahai, Erik","last_name":"Sahai","first_name":"Erik"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"},{"last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"}],"oa":1,"has_accepted_license":"1","month":"09","date_updated":"2026-10-25T23:31:25Z","ddc":["539","570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2025-09-25T00:00:00Z","OA_place":"repository","das_tickbox":"1","day":"25","_id":"21427","language":[{"iso":"eng"}],"title":"Substrate heterogeneity promotes cancer cell dissemination through interface roughening"},{"file":[{"content_type":"application/pdf","access_level":"open_access","date_updated":"2025-04-16T10:54:07Z","date_created":"2025-04-16T10:54:07Z","relation":"main_file","creator":"dernst","checksum":"bb58db4a908a1f4aabe4004706154541","file_size":6994499,"file_name":"2025_DevelopmentalCell_Lehr.pdf","success":1,"file_id":"19584"}],"doi":"10.1016/j.devcel.2024.10.024","acknowledgement":"We thank A. Miller and N. Papalopulu for reagents and J. Briscoe for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European Research Council under Horizon Europe, grant 101044579; and the Austrian Science Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022).","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"19763"}]},"quality_controlled":"1","article_processing_charge":"Yes (via OA deal)","researchdata_availability":"yes","publication":"Developmental Cell","project":[{"_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","grant_number":"101044579","name":"Mechanisms of tissue size regulation in spinal cord development"},{"grant_number":"F7802","name":"Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen control of growth and pattern in the spinal cord","_id":"059DF620-7A3F-11EA-A408-12923DDC885E"},{"_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-011","name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube"}],"abstract":[{"text":"Developing tissues interpret dynamic changes in morphogen activity to generate cell type diversity. To quantitatively study bone morphogenetic protein (BMP) signaling dynamics in the mouse neural tube, we developed an embryonic stem cell differentiation system tailored for growing tissues. Differentiating cells form striking self-organized patterns of dorsal neural tube cell types driven by sequential phases of BMP signaling that are observed both in vitro and in vivo. Data-driven biophysical modeling showed that these dynamics result from coupling fast negative feedback with slow positive regulation of signaling by the specification of an endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling in space, we identify a BMP signaling relay that operates in time. This mechanism allows for a rapid initial concentration-sensitive response that is robustly terminated, thereby regulating balanced sequential cell type generation. Our study provides an experimental and theoretical framework to understand how signaling dynamics are exploited in developing tissues.","lang":"eng"}],"article_type":"original","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Cell Press","scopus_import":"1","dataavailabilitystatement":"RNA-seq data has been deposited at GEO and are publicly available as of the date of publication. Accession numbers are listed in the key resources table. Code for the quantification of fluorescence intensity (FI) profiles in differentiated cells on stencils, as well as for model simulations, is available on GitHub (https://github.com/dbrueckner/NeuralTubeColonies, https://doi.org/10.5281/zenodo.13335390","fulldoi":"https://doi.org/10.1016/j.devcel.2024.10.024","type":"journal_article","page":"567-580","volume":60,"corr_author":"1","publication_status":"published","oa_version":"Published Version","status":"public","citation":{"chicago":"Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>. Cell Press, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>.","ista":"Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva A. 2025. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.","ieee":"S. Rus <i>et al.</i>, “Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling,” <i>Developmental Cell</i>, vol. 60, no. 4. Cell Press, pp. 567–580, 2025.","ama":"Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. 2025;60(4):567-580. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>","short":"S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo, A. Kicheva, Developmental Cell 60 (2025) 567–580.","apa":"Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E. B., &#38; Kicheva, A. (2025). Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">https://doi.org/10.1016/j.devcel.2024.10.024</a>","mla":"Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” <i>Developmental Cell</i>, vol. 60, no. 4, Cell Press, 2025, pp. 567–80, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.024\">10.1016/j.devcel.2024.10.024</a>."},"date_created":"2025-01-09T11:25:47Z","year":"2025","OA_type":"hybrid","department":[{"_id":"AnKi"},{"_id":"EdHa"},{"_id":"NanoFab"}],"external_id":{"isi":["001434279000001"],"pmid":["39603235"]},"month":"02","oa":1,"has_accepted_license":"1","author":[{"full_name":"Rus, Stefanie","orcid":"0000-0001-8703-1093","first_name":"Stefanie","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","last_name":"Rus"},{"orcid":"0000-0001-7205-2975","full_name":"Brückner, David","first_name":"David","last_name":"Brückner","id":"e1e86031-6537-11eb-953a-f7ab92be508d"},{"last_name":"Minchington","id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f","first_name":"Thomas","full_name":"Minchington, Thomas"},{"full_name":"Greunz, Martina","last_name":"Greunz","id":"48A59534-F248-11E8-B48F-1D18A9856A87","first_name":"Martina"},{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","first_name":"Jack"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B"},{"id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","last_name":"Kicheva","first_name":"Anna","full_name":"Kicheva, Anna","orcid":"0000-0003-4509-4998"}],"das_tickbox":"1","OA_place":"publisher","file_date_updated":"2025-04-16T10:54:07Z","publication_identifier":{"issn":["1534-5807"]},"intvolume":"        60","supplementarymaterial":"yes","date_updated":"2026-10-25T23:31:29Z","ddc":["570"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2025-02-24T00:00:00Z","title":"Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling","_id":"18807","language":[{"iso":"eng"}],"day":"24","isi":1,"issue":"4","pmid":1},{"department":[{"_id":"CaHe"},{"_id":"EdHa"},{"_id":"MaLo"},{"_id":"NanoFab"}],"citation":{"short":"F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current Biology 34 (2024) 171–182.e8.","apa":"Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., &#38; Heisenberg, C.-P. J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>","ama":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. <i>Current Biology</i>. 2024;34(1):171-182.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>","mla":"Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">10.1016/j.cub.2023.11.067</a>.","ieee":"F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg, “Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” <i>Current Biology</i>, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.","ista":"Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1), 171–182.e8.","chicago":"Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell Contacts.” <i>Current Biology</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.cub.2023.11.067\">https://doi.org/10.1016/j.cub.2023.11.067</a>."},"date_created":"2024-01-14T23:00:56Z","year":"2024","status":"public","author":[{"id":"49DA7910-F248-11E8-B48F-1D18A9856A87","last_name":"Arslan","first_name":"Feyza N","full_name":"Arslan, Feyza N","orcid":"0000-0001-5809-9566"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"first_name":"Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"full_name":"Loose, Martin","orcid":"0000-0001-7309-9724","first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"has_accepted_license":"1","oa":1,"month":"01","external_id":{"isi":["001154500400001"],"pmid":["38134934"]},"date_published":"2024-01-08T00:00:00Z","ddc":["570"],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2026-10-01T08:33:05Z","file_date_updated":"2024-01-16T10:53:31Z","supplementarymaterial":"yes","publication_identifier":{"issn":["0960-9822"],"eissn":["1879-0445"]},"intvolume":"        34","das_tickbox":"1","day":"08","isi":1,"pmid":1,"issue":"1","_id":"14795","language":[{"iso":"eng"}],"title":"Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts","acknowledgement":"We are grateful to Edwin Munro for their feedback and help with the single particle analysis. We thank members of the Heisenberg and Loose labs for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA for their continuous support, especially Yann Cesbron for assistance with the laser cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H.","doi":"10.1016/j.cub.2023.11.067","file":[{"date_updated":"2024-01-16T10:53:31Z","date_created":"2024-01-16T10:53:31Z","content_type":"application/pdf","access_level":"open_access","file_name":"2024_CurrentBiology_Arslan.pdf","file_size":5183861,"creator":"dernst","checksum":"51220b76d72a614208f84bdbfbaf9b72","file_id":"14813","success":1,"relation":"main_file"}],"article_type":"original","abstract":[{"lang":"eng","text":"Metazoan development relies on the formation and remodeling of cell-cell contacts. Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in space and time plays a central role in cell-cell contact formation and maturation. Nevertheless, how this process is mechanistically achieved when new contacts are formed remains unclear. Here, by building a biomimetic assay composed of progenitor cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains, we show that cortical F-actin flows, driven by the depletion of myosin-2 at the cell contact center, mediate the dynamic reorganization of adhesion receptors and cell cortex at the contact. E-cadherin-dependent downregulation of the small GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2 becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical tension gradient from the contact rim to its center. This tension gradient, in turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin at the contact rim and the progressive redistribution of E-cadherin from the contact center to the rim. Eventually, this combination of actomyosin downregulation and flows at the contact determines the characteristic molecular organization, with E-cadherin and F-actin accumulating at the contact rim, where they are needed to mechanically link the contractile cortices of the adhering cells."}],"researchdata_availability":"upon request","publication":"Current Biology","project":[{"_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573","call_identifier":"H2020"}],"article_processing_charge":"Yes (via OA deal)","quality_controlled":"1","fulldoi":"https://doi.org/10.1016/j.cub.2023.11.067","ec_funded":1,"type":"journal_article","dataavailabilitystatement":"All original microscopy data any additional information reported in this paper is available from the lead contact upon request. All original code is available from the lead contact upon request","scopus_import":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Elsevier","publication_status":"published","oa_version":"Published Version","corr_author":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"page":"171-182.e8","volume":34},{"oa_version":"Published Version","publication_status":"published","volume":16,"article_number":"097","type":"journal_article","fulldoi":"https://doi.org/10.21468/scipostphys.16.4.097","dataavailabilitystatement":"Source code, simulation data, and analysis scripts will be made publicly available on zenodo.org (10.5281/zenodo.8410957) after acceptance of the manuscript.","scopus_import":"1","publisher":"SciPost Foundation","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"abstract":[{"text":"The homeostasis of epithelial tissue relies on a balance between the self-renewal of stem cell populations, cellular differentiation, and loss. Although this balance needs to be tightly regulated to avoid pathologies, such as tumor growth, the regulatory mechanisms, both cell-intrinsic and collective, which ensure tissue steady-state are still poorly understood. Here, we develop a computational model that incorporates basic assumptions of stem cell renewal into distinct populations and mechanical interactions between cells. We find that the model generates unexpected dynamic features: stem cells repel each other in the bulk tissue and are thus found rather isolated, as in a number of in vivo contexts. By mapping the system onto a gas of passive Brownian particles with effective repulsive interactions, that arise from the generated flows of differentiated cells, we show that we can quantitatively describe such stem cell distribution in tissues. The interaction potential between a pair of stem cells decays exponentially with a characteristic length that spans several cell sizes, corresponding to the volume of cells generated per stem cell division. Our findings may help understanding the dynamics of normal and cancerous epithelial tissues.","lang":"eng"}],"article_type":"original","publication":"SciPost Physics","researchdata_availability":"yes","article_processing_charge":"No","quality_controlled":"1","acknowledgement":"JE and JK gratefully acknowledge financial support from the Initiative and Networking Fund (IVF) via the grant number ERC-RA-004. Simulations were performed with computing resources granted by RWTH Aachen University under project ‘rwth0475’.","related_material":{"record":[{"id":"23022","relation":"research_data","status":"public"}]},"doi":"10.21468/scipostphys.16.4.097","file":[{"relation":"main_file","creator":"dernst","file_name":"2024_SciPostPhys_Kraemer.pdf","checksum":"6fdeecd21c166db8dedb927ecc2e6025","file_size":4973291,"success":1,"file_id":"17109","content_type":"application/pdf","access_level":"open_access","date_created":"2024-06-03T11:18:51Z","date_updated":"2024-06-03T11:18:51Z"}],"isi":1,"issue":"4","day":"08","_id":"17104","language":[{"iso":"eng"}],"arxiv":1,"title":"Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells","date_published":"2024-04-08T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","date_updated":"2026-10-01T09:44:06Z","ddc":["530"],"supplementarymaterial":"yes","publication_identifier":{"issn":["2542-4653"]},"intvolume":"        16","file_date_updated":"2024-06-03T11:18:51Z","das_tickbox":"1","author":[{"last_name":"Krämer","first_name":"Johannes C.","full_name":"Krämer, Johannes C."},{"first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B"},{"last_name":"Gompper","first_name":"Gerhard","full_name":"Gompper, Gerhard"},{"full_name":"Elgeti, Jens","first_name":"Jens","last_name":"Elgeti"}],"has_accepted_license":"1","oa":1,"month":"04","external_id":{"arxiv":["2310.04272"],"isi":["001202370200001"]},"department":[{"_id":"EdHa"}],"year":"2024","date_created":"2024-06-03T08:58:44Z","citation":{"ieee":"J. C. Krämer, E. B. Hannezo, G. Gompper, and J. Elgeti, “Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells,” <i>SciPost Physics</i>, vol. 16, no. 4. SciPost Foundation, 2024.","chicago":"Krämer, Johannes C., Edouard B Hannezo, Gerhard Gompper, and Jens Elgeti. “Mechanically-Driven Stem Cell Separation in Tissues Caused by Proliferating Daughter Cells.” <i>SciPost Physics</i>. SciPost Foundation, 2024. <a href=\"https://doi.org/10.21468/scipostphys.16.4.097\">https://doi.org/10.21468/scipostphys.16.4.097</a>.","ista":"Krämer JC, Hannezo EB, Gompper G, Elgeti J. 2024. Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells. SciPost Physics. 16(4), 097.","mla":"Krämer, Johannes C., et al. “Mechanically-Driven Stem Cell Separation in Tissues Caused by Proliferating Daughter Cells.” <i>SciPost Physics</i>, vol. 16, no. 4, 097, SciPost Foundation, 2024, doi:<a href=\"https://doi.org/10.21468/scipostphys.16.4.097\">10.21468/scipostphys.16.4.097</a>.","short":"J.C. Krämer, E.B. Hannezo, G. Gompper, J. Elgeti, SciPost Physics 16 (2024).","apa":"Krämer, J. C., Hannezo, E. B., Gompper, G., &#38; Elgeti, J. (2024). Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells. <i>SciPost Physics</i>. SciPost Foundation. <a href=\"https://doi.org/10.21468/scipostphys.16.4.097\">https://doi.org/10.21468/scipostphys.16.4.097</a>","ama":"Krämer JC, Hannezo EB, Gompper G, Elgeti J. Mechanically-driven stem cell separation in tissues caused by proliferating daughter cells. <i>SciPost Physics</i>. 2024;16(4). doi:<a href=\"https://doi.org/10.21468/scipostphys.16.4.097\">10.21468/scipostphys.16.4.097</a>"},"status":"public"}]