---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '21015'
abstract:
- lang: eng
text: Early embryo geometry is one of the most invariant species-specific traits,
yet its role in ensuring developmental reproducibility and robustness remains
underexplored. Here we show that in zebrafish, the geometry of the fertilized
egg—specifically its curvature and volume—serves as a critical initial condition
triggering a cascade of events that influence development. The embryo geometry
guides patterned asymmetric cell divisions in the blastoderm, generating radial
gradients of cell volume and nucleocytoplasmic ratio. These gradients generate
mitotic phase waves, with the nucleocytoplasmic ratio determining individual cell
cycle periods independently of other cells. We demonstrate that reducing cell
autonomy reshapes these waves, emphasizing the instructive role of geometry-derived
volume patterns in setting the intrinsic period of the cell cycle oscillator.
In addition to organizing cell cycles, early embryo geometry spatially patterns
zygotic genome activation at the midblastula transition, a key step in establishing
embryonic autonomy. Disrupting the embryo shape alters the zygotic genome activation
pattern and causes ectopic germ layer specification, underscoring the developmental
significance of geometry. Together, our findings reveal a symmetry-breaking function
of early embryo geometry in coordinating cell cycle and transcriptional patterning.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
- _id: ScienComp
- _id: LifeSc
acknowledgement: We thank N. Petridou (EMBL) for sharing results before publication.
N.M. was supported by funding from the European Union’s Horizon 2020 programme under
the Marie Skłodowska-Curie COFUND Actions ISTplus grant agreement number 754411.
Y.I.L. acknowledges funding from the European Union’s Horizon 2020 research and
innovation programme under the Marie Skłodowska-Curie grant agreement number 101034413.
The research was supported by funding to C.-P.H. from the NOMIS Foundation, Project
ID 1.844. We would like to thank past and present members of the Heisenberg and
Hannezo groups for discussions, particularly S. Shamipour, V. Doddihal, M. Jovic,
N. Hino, F. N. Arslan, R. Kobylinska and C. Camelo for feedback on the draft manuscript.
This research was supported by the Scientific Service Units (SSU) of Institute of
Science and Technology Austria through resources provided by the Aquatics Facility,
Imaging & Optics Facility (IOF), Scientific Computing (SciComp) facility and Lab
Support Facility (LSF). Open access funding provided by Institute of Science and
Technology (IST Austria).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Nikhil
full_name: Mishra, Nikhil
id: C4D70E82-1081-11EA-B3ED-9A4C3DDC885E
last_name: Mishra
orcid: 0000-0002-6425-5788
- first_name: Yuting I
full_name: Li, Yuting I
id: ee7a5ca8-8b71-11ed-b662-b3341c05b7eb
last_name: Li
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. Geometry-driven asymmetric cell
divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo.
Nature Physics. 2026;22:139-150. doi:10.1038/s41567-025-03122-1
apa: Mishra, N., Li, Y. I., Hannezo, E. B., & Heisenberg, C.-P. J. (2026). Geometry-driven
asymmetric cell divisions pattern cell cycles and zygotic genome activation in
the zebrafish embryo. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-025-03122-1
chicago: Mishra, Nikhil, Yuting I Li, Edouard B Hannezo, and Carl-Philipp J Heisenberg.
“Geometry-Driven Asymmetric Cell Divisions Pattern Cell Cycles and Zygotic Genome
Activation in the Zebrafish Embryo.” Nature Physics. Springer Nature, 2026.
https://doi.org/10.1038/s41567-025-03122-1.
ieee: N. Mishra, Y. I. Li, E. B. Hannezo, and C.-P. J. Heisenberg, “Geometry-driven
asymmetric cell divisions pattern cell cycles and zygotic genome activation in
the zebrafish embryo,” Nature Physics, vol. 22. Springer Nature, pp. 139–150,
2026.
ista: Mishra N, Li YI, Hannezo EB, Heisenberg C-PJ. 2026. Geometry-driven asymmetric
cell divisions pattern cell cycles and zygotic genome activation in the zebrafish
embryo. Nature Physics. 22, 139–150.
mla: Mishra, Nikhil, et al. “Geometry-Driven Asymmetric Cell Divisions Pattern Cell
Cycles and Zygotic Genome Activation in the Zebrafish Embryo.” Nature Physics,
vol. 22, Springer Nature, 2026, pp. 139–50, doi:10.1038/s41567-025-03122-1.
short: N. Mishra, Y.I. Li, E.B. Hannezo, C.-P.J. Heisenberg, Nature Physics 22 (2026)
139–150.
corr_author: '1'
date_created: 2026-01-20T10:12:19Z
date_published: 2026-01-05T00:00:00Z
date_updated: 2026-04-28T12:55:30Z
day: '05'
ddc:
- '570'
department:
- _id: EdHa
- _id: CaHe
doi: 10.1038/s41567-025-03122-1
ec_funded: 1
external_id:
oaworkid:
- W7118187193
file:
- access_level: open_access
checksum: 0ab7ac2fbcb61a364dba57152db64ed7
content_type: application/pdf
creator: dernst
date_created: 2026-01-21T08:21:11Z
date_updated: 2026-01-21T08:21:11Z
file_id: '21026'
file_name: 2026_NaturePhysics_Mishra.pdf
file_size: 7335694
relation: main_file
success: 1
file_date_updated: 2026-01-21T08:21:11Z
has_accepted_license: '1'
intvolume: ' 22'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
oaworkid: 1
page: 139-150
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
call_identifier: H2020
grant_number: '101034413'
name: 'IST-BRIDGE: International postdoctoral program'
- _id: 917c023a-16d5-11f0-9cad-eb5cafc52090
name: Cytoplasmic self-organization into cell-like compartments as a common guiding
principle in early animal development
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
issnl:
- ' 1745-2473'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: research_data
url: https://ista.ac.at/en/news/geometry-shapes-life/
scopus_import: '1'
status: public
title: Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome
activation in the zebrafish embryo
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 22
year: '2026'
...
---
OA_place: repository
_id: '21427'
abstract:
- lang: eng
text: While tumor malignancy has been extensively studied under the prism of genetic
and epigenetic heterogeneity, tumor cell states also critically depend on reciprocal
interactions with the microenvironment. This raises the hitherto untested possibility
that heterogeneity of the untransformed tumor stroma can actively fuel malignant
progression. As biological heterogeneity is inherently difficult to control, we
adopted a reductionist approach and let tumor cells invade micro-engineered environments
harboring obstacles with precision-controlled geometry. We find that not only
the presence of obstacles, but more surprisingly their spatial disorder, causes
a drastic shift from a collective to a single-cell mode of invasion – comparable
in strength to cadherin loss. Combining live-imaging and perturbation experiments
with minimal biophysical modeling, we demonstrate that cell detachments result
both from local geometrical constraints and a global integration of spatial disorder
over time. We show that different types of microenvironments map onto different
universality classes of invasion dynamics - homogeneous substrates follow Kardar–Parisi–Zhang
(KPZ) scaling, while disordered ones exhibit exponents consistent with KPZ with
quenched disorder (KPZq). Our findings highlight generic physical principles for
how the mode of cancer cell invasion depends on environmental heterogeneity, with
potential implications to understand tumor evolution in vivo.
acknowledgement: "European Research Council, https://ror.org/0472cxd90, 101071793\r\nAustrian
Academy of Sciences, 26360"
article_processing_charge: No
author:
- first_name: Zuzana
full_name: Dunajova, Zuzana
id: 4B39F286-F248-11E8-B48F-1D18A9856A87
last_name: Dunajova
- first_name: Saren
full_name: Tasciyan, Saren
id: 4323B49C-F248-11E8-B48F-1D18A9856A87
last_name: Tasciyan
orcid: 0000-0003-1671-393X
- first_name: Juraj
full_name: Majek, Juraj
id: 3e6d9473-f38e-11ec-8ae0-c4e05a8aa9e1
last_name: Majek
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Erik
full_name: Sahai, Erik
last_name: Sahai
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Dunajova Z, Tasciyan S, Majek J, et al. Substrate heterogeneity promotes cancer
cell dissemination through interface roughening. doi:10.1101/2025.05.20.655037
apa: Dunajova, Z., Tasciyan, S., Majek, J., Merrin, J., Sahai, E., Sixt, M. K.,
& Hannezo, E. B. (n.d.). Substrate heterogeneity promotes cancer cell dissemination
through interface roughening. bioRxiv. https://doi.org/10.1101/2025.05.20.655037
chicago: Dunajova, Zuzana, Saren Tasciyan, Juraj Majek, Jack Merrin, Erik Sahai,
Michael K Sixt, and Edouard B Hannezo. “Substrate Heterogeneity Promotes Cancer
Cell Dissemination through Interface Roughening.” bioRxiv, n.d. https://doi.org/10.1101/2025.05.20.655037.
ieee: Z. Dunajova et al., “Substrate heterogeneity promotes cancer cell dissemination
through interface roughening.” bioRxiv.
ista: Dunajova Z, Tasciyan S, Majek J, Merrin J, Sahai E, Sixt MK, Hannezo EB. Substrate
heterogeneity promotes cancer cell dissemination through interface roughening.
10.1101/2025.05.20.655037.
mla: Dunajova, Zuzana, et al. Substrate Heterogeneity Promotes Cancer Cell Dissemination
through Interface Roughening. bioRxiv, doi:10.1101/2025.05.20.655037.
short: Z. Dunajova, S. Tasciyan, J. Majek, J. Merrin, E. Sahai, M.K. Sixt, E.B.
Hannezo, (n.d.).
corr_author: '1'
date_created: 2026-03-11T08:40:06Z
date_published: 2025-09-25T00:00:00Z
date_updated: 2026-06-10T09:41:11Z
day: '25'
ddc:
- '539'
- '570'
department:
- _id: GradSch
- _id: EdHa
- _id: MiSi
- _id: NanoFab
- _id: AnSa
doi: 10.1101/2025.05.20.655037
has_accepted_license: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2025.05.20.655037
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: bd91e723-d553-11ed-ba76-fe7eeb2185fd
grant_number: '101071793'
name: 'Pushing from within: Control of cell shape, integrity and motility by cytoskeletal
pushing forces'
- _id: 34d75525-11ca-11ed-8bc3-89b6307fee9d
grant_number: '26360'
name: Motile active matter models of migrating cells and chiral filaments
publication_status: draft
publisher: bioRxiv
related_material:
record:
- id: '21423'
relation: dissertation_contains
status: public
- id: '21439'
relation: research_data
status: public
status: public
title: Substrate heterogeneity promotes cancer cell dissemination through interface
roughening
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: preprint
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2025'
...
---
OA_type: closed access
_id: '18960'
abstract:
- lang: eng
text: The importance of physical forces in the morphogenesis, homeostatic function,
and pathological dysfunction of multicellular tissues is being increasingly characterized,
both theoretically and experimentally. Analogies between biological systems and
inert materials such as foams, gels, and liquid crystals have provided striking
insights into the core design principles underlying multicellular organization.
However, these connections can seem surprising given that a key feature of multicellular
systems is their ability to constantly consume energy, providing an active origin
for the forces that they produce. Key emerging questions are, therefore, to understand
whether and how this activity grants tissues novel properties that do not have
counterparts in classical materials, as well as their consequences for biological
function. Here, we review recent discoveries at the intersection of active matter
and tissue biology, with an emphasis on how modeling and experiments can be combined
to understand the dynamics of multicellular systems. These approaches suggest
that a number of key biological tissue-scale phenomena, such as morphogenetic
shape changes, collective migration, or fate decisions, share unifying design
principles that can be described by physical models of tissue active matter.
acknowledgement: We thank Fridtjof Brauns, Anna Kicheva, and Carl-Philipp Heisenberg
for a critical reading of the manuscript and Claudia Flandoli for the artwork in
the figures. D.B.B. was supported by the NOMIS foundation as a NOMIS Fellow and
by an EMBO Postdoctoral Fellowship (ALTF 343-2022). This work received funding from
the European Research Council (ERC) under the European Union\u2019s Horizon 2020
Research and Innovation Programme Grant Agreement no. 851288.
article_number: a041653
article_processing_charge: No
article_type: original
author:
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: 'Brückner D, Hannezo EB. Tissue active matter: Integrating mechanics and signaling
into dynamical models. Cold Spring Harbor Perspectives in Biology. 2025;17(4).
doi:10.1101/cshperspect.a041653'
apa: 'Brückner, D., & Hannezo, E. B. (2025). Tissue active matter: Integrating
mechanics and signaling into dynamical models. Cold Spring Harbor Perspectives
in Biology. Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/cshperspect.a041653'
chicago: 'Brückner, David, and Edouard B Hannezo. “Tissue Active Matter: Integrating
Mechanics and Signaling into Dynamical Models.” Cold Spring Harbor Perspectives
in Biology. Cold Spring Harbor Laboratory Press, 2025. https://doi.org/10.1101/cshperspect.a041653.'
ieee: 'D. Brückner and E. B. Hannezo, “Tissue active matter: Integrating mechanics
and signaling into dynamical models,” Cold Spring Harbor Perspectives in Biology,
vol. 17, no. 4. Cold Spring Harbor Laboratory Press, 2025.'
ista: 'Brückner D, Hannezo EB. 2025. Tissue active matter: Integrating mechanics
and signaling into dynamical models. Cold Spring Harbor Perspectives in Biology.
17(4), a041653.'
mla: 'Brückner, David, and Edouard B. Hannezo. “Tissue Active Matter: Integrating
Mechanics and Signaling into Dynamical Models.” Cold Spring Harbor Perspectives
in Biology, vol. 17, no. 4, a041653, Cold Spring Harbor Laboratory Press,
2025, doi:10.1101/cshperspect.a041653.'
short: D. Brückner, E.B. Hannezo, Cold Spring Harbor Perspectives in Biology 17
(2025).
corr_author: '1'
date_created: 2025-01-29T13:33:47Z
date_published: 2025-04-01T00:00:00Z
date_updated: 2025-12-30T07:08:34Z
day: '01'
department:
- _id: EdHa
doi: 10.1101/cshperspect.a041653
ec_funded: 1
external_id:
isi:
- '001456660400001'
pmid:
- '38951023'
intvolume: ' 17'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
pmid: 1
project:
- _id: 34e2a5b5-11ca-11ed-8bc3-b2265616ef0b
grant_number: ALTF 343-2022
name: A mechano-chemical theory for stem cell fate decisions in organoid development
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Cold Spring Harbor Perspectives in Biology
publication_identifier:
issn:
- 1943-0264
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Tissue active matter: Integrating mechanics and signaling into dynamical models'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 17
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '19373'
abstract:
- lang: eng
text: Reproducible pattern and form generation during embryogenesis is poorly understood.
Intestinal organoid morphogenesis involves a number of mechanochemical regulators
such as cell-type-specific cytoskeletal forces and osmotically driven lumen volume
changes. It is unclear how these forces are coordinated in time and space to ensure
robust morphogenesis. Here we show how mechanosensitive feedback on cytoskeletal
tension gives rise to morphological bistability in a minimal model of organoid
morphogenesis. In the model, lumen volume changes can impact the epithelial shape
via both direct mechanical and indirect mechanosensitive mechanisms. We find that
both bulged and budded crypt states are possible and dependent on the history
of volume changes. We test key modelling assumptions via biophysical and pharmacological
experiments to demonstrate how bistability can explain experimental observations,
such as the importance of the timing of lumen shrinkage and robustness of the
final morphogenetic state to mechanical perturbations. This suggests that bistability
arising from feedback between cellular tensions and fluid pressure could be a
general mechanism that coordinates multicellular shape changes in developing systems.
acknowledgement: We thank all members of the Hannezo and Liberali groups for fruitful
discussions, as well as C. Schwayer, G. Quintas, L. Capolupo, D. Bruckner and D.
Pinheiro for reading the manuscript. We also thank Y. Wu and X. Wu from the Yang
group for performing experiments in the last rounds of revision and the So group
at the National Institute of Biological Sciences, Beijing, for helping with the
light-sheet time-lapse experiments. This work received funding from the European
Research Council (ERC) under the European Union’s Horizon 2020 research and innovation
programme via grant agreement no. 758617 (to P.L.), Swiss National Foundation (SNF)
(no. POOP3_157531 to P.L.), the ERC under the European Union’s Horizon 2020 research
and innovation programme under grant agreement no. 851288 (to E.H.) and the Austrian
Science Fund (FWF) (no. P 31639 to E.H.). This work was supported by the National
Natural Science Foundation of China via grant no.3247060387 (to Q.Y.) and the Strategic
Priority Research Program of the Chinese Academy of Sciences (no. XDB0820000 to
Q.Y.) . Open access funding provided by Institute of Science and Technology (IST
Austria).
article_number: '078104'
article_processing_charge: Yes (via OA deal)
article_type: original
arxiv: 1
author:
- first_name: Shi-lei
full_name: Xue, Shi-lei
id: 31D2C804-F248-11E8-B48F-1D18A9856A87
last_name: Xue
- first_name: Qiutan
full_name: Yang, Qiutan
last_name: Yang
- first_name: Prisca
full_name: Liberali, Prisca
last_name: Liberali
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Xue S, Yang Q, Liberali P, Hannezo EB. Mechanochemical bistability of intestinal
organoids enables robust morphogenesis. Nature Physics. 2025;21. doi:10.1038/s41567-025-02792-1
apa: Xue, S., Yang, Q., Liberali, P., & Hannezo, E. B. (2025). Mechanochemical
bistability of intestinal organoids enables robust morphogenesis. Nature Physics.
Springer Nature. https://doi.org/10.1038/s41567-025-02792-1
chicago: Xue, Shi-lei, Qiutan Yang, Prisca Liberali, and Edouard B Hannezo. “Mechanochemical
Bistability of Intestinal Organoids Enables Robust Morphogenesis.” Nature Physics.
Springer Nature, 2025. https://doi.org/10.1038/s41567-025-02792-1.
ieee: S. Xue, Q. Yang, P. Liberali, and E. B. Hannezo, “Mechanochemical bistability
of intestinal organoids enables robust morphogenesis,” Nature Physics,
vol. 21. Springer Nature, 2025.
ista: Xue S, Yang Q, Liberali P, Hannezo EB. 2025. Mechanochemical bistability of
intestinal organoids enables robust morphogenesis. Nature Physics. 21, 078104.
mla: Xue, Shi-lei, et al. “Mechanochemical Bistability of Intestinal Organoids Enables
Robust Morphogenesis.” Nature Physics, vol. 21, 078104, Springer Nature,
2025, doi:10.1038/s41567-025-02792-1.
short: S. Xue, Q. Yang, P. Liberali, E.B. Hannezo, Nature Physics 21 (2025).
corr_author: '1'
date_created: 2025-03-09T23:01:28Z
date_published: 2025-02-28T00:00:00Z
date_updated: 2025-09-30T10:47:36Z
day: '28'
ddc:
- '530'
department:
- _id: EdHa
doi: 10.1038/s41567-025-02792-1
ec_funded: 1
external_id:
arxiv:
- '2403.19900'
isi:
- '001434072800001'
pmid:
- '40248571'
file:
- access_level: open_access
checksum: fb5e59be145b95f9851d3d7c9dbb85e6
content_type: application/pdf
creator: dernst
date_created: 2025-08-05T12:12:03Z
date_updated: 2025-08-05T12:12:03Z
file_id: '20129'
file_name: 2025_NaturePhysics_Xue.pdf
file_size: 16302436
relation: main_file
success: 1
file_date_updated: 2025-08-05T12:12:03Z
has_accepted_license: '1'
intvolume: ' 21'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
- _id: 268294B6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P31639
name: Active mechano-chemical description of the cell cytoskeleton
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanochemical bistability of intestinal organoids enables robust morphogenesis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 21
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '19507'
abstract:
- lang: eng
text: The epidermis provides a protective barrier against hostile environments.
However, our knowledge of how this barrier forms during development and is subsequently
maintained remains incomplete. The infundibulum is a cylindrical epidermal tissue
compartment that serves as an outlet for hair follicles protruding from the skin
and the excretion of the sebaceous glands that are essential for proper skin function.
In this study, we applied quantitative fate mapping to address how infundibulum
are maintained during adulthood. We demonstrate that progenitors build and maintain
tissues through stochastic cell fate choices. Long-term analysis identified a
preferential transient contribution from cells initially located at the bottom
of the structure to the maintenance of the tissue, with bursts of local progenitor
expansion associated with the phases of hair growth. Beyond providing compartment-wide
insights into progenitor cell dynamics in infundibulum, these findings demonstrate
how spatiotemporal regulation controls transient progenitor dominance.
acknowledgement: We thank the members of the Jensen Laboratory for experimental and
technical advice, the imaging facilities at reNEW, and animal caretakers for expert
assistance. This work was supported by the Lundbeck Foundation (R105-A9755 to KBJ)
and the Leo Pharma Foundation (LF-OC-20-000169). The Novo Nordisk Foundation Center
for Stem Cell Medicine was supported by a Novo Nordisk Foundation grant (NNF21CC0073729).
B.D.S. was supported by the Wellcome Trust (219478/Z/19/Z) and a Royal Society EP
Abraham Research Professorship (RP/R1/180165 and RP\R\231004). Figure elements were
adapted from Bio-Render. KBJ is the lead contact and guarantor of this study.
article_processing_charge: No
article_type: original
author:
- first_name: Marianne S.
full_name: Andersen, Marianne S.
last_name: Andersen
- first_name: Svetlana
full_name: Ulyanchenko, Svetlana
last_name: Ulyanchenko
- first_name: Pawel J.
full_name: Schweiger, Pawel J.
last_name: Schweiger
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Benjamin D.
full_name: Simons, Benjamin D.
last_name: Simons
- first_name: Kim B.
full_name: Jensen, Kim B.
last_name: Jensen
citation:
ama: Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB.
Spatiotemporal switches in progenitor cell fate govern upper hair follicle growth
and maintenance. Journal of Investigative Dermatology. 2025;145(9):2191-2202.e5.
doi:10.1016/j.jid.2025.01.034
apa: Andersen, M. S., Ulyanchenko, S., Schweiger, P. J., Hannezo, E. B., Simons,
B. D., & Jensen, K. B. (2025). Spatiotemporal switches in progenitor cell
fate govern upper hair follicle growth and maintenance. Journal of Investigative
Dermatology. Elsevier. https://doi.org/10.1016/j.jid.2025.01.034
chicago: Andersen, Marianne S., Svetlana Ulyanchenko, Pawel J. Schweiger, Edouard
B Hannezo, Benjamin D. Simons, and Kim B. Jensen. “Spatiotemporal Switches in
Progenitor Cell Fate Govern Upper Hair Follicle Growth and Maintenance.” Journal
of Investigative Dermatology. Elsevier, 2025. https://doi.org/10.1016/j.jid.2025.01.034.
ieee: M. S. Andersen, S. Ulyanchenko, P. J. Schweiger, E. B. Hannezo, B. D. Simons,
and K. B. Jensen, “Spatiotemporal switches in progenitor cell fate govern upper
hair follicle growth and maintenance,” Journal of Investigative Dermatology,
vol. 145, no. 9. Elsevier, p. 2191–2202.e5, 2025.
ista: Andersen MS, Ulyanchenko S, Schweiger PJ, Hannezo EB, Simons BD, Jensen KB.
2025. Spatiotemporal switches in progenitor cell fate govern upper hair follicle
growth and maintenance. Journal of Investigative Dermatology. 145(9), 2191–2202.e5.
mla: Andersen, Marianne S., et al. “Spatiotemporal Switches in Progenitor Cell Fate
Govern Upper Hair Follicle Growth and Maintenance.” Journal of Investigative
Dermatology, vol. 145, no. 9, Elsevier, 2025, p. 2191–2202.e5, doi:10.1016/j.jid.2025.01.034.
short: M.S. Andersen, S. Ulyanchenko, P.J. Schweiger, E.B. Hannezo, B.D. Simons,
K.B. Jensen, Journal of Investigative Dermatology 145 (2025) 2191–2202.e5.
corr_author: '1'
date_created: 2025-04-06T22:01:32Z
date_published: 2025-09-01T00:00:00Z
date_updated: 2025-12-29T14:13:43Z
day: '01'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1016/j.jid.2025.01.034
external_id:
isi:
- '001604396400001'
pmid:
- '40010488'
file:
- access_level: open_access
checksum: a2b313de3cacb53f20f2b91c42612ad9
content_type: application/pdf
creator: dernst
date_created: 2025-12-29T14:13:01Z
date_updated: 2025-12-29T14:13:01Z
file_id: '20874'
file_name: 2025_JourInvestigativeDerma_Andersen.pdf
file_size: 7301679
relation: main_file
success: 1
file_date_updated: 2025-12-29T14:13:01Z
has_accepted_license: '1'
intvolume: ' 145'
isi: 1
issue: '9'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 2191-2202.e5
pmid: 1
publication: Journal of Investigative Dermatology
publication_identifier:
eissn:
- 1523-1747
issn:
- 0022-202X
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Spatiotemporal switches in progenitor cell fate govern upper hair follicle
growth and maintenance
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 145
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '19703'
abstract:
- lang: eng
text: An enlarged brain underlies the complex central nervous system of vertebrates.
The dramatic expansion of the brain that diverges its shape from the spinal cord
follows neural tube closure during embryonic development. Here, we show that this
differential deformation is encoded by a pre-pattern of tissue material properties
in chicken embryos. Using magnetic droplets and atomic force microscopy, we demonstrate
that the dorsal hindbrain is more fluid than the dorsal spinal cord, resulting
in a thinning versus a resisting response to increasing lumen pressure, respectively.
The dorsal hindbrain exhibits reduced apical actin and a disorganized laminin
matrix consistent with tissue fluidization. Blocking the activity of neural-crest-associated
matrix metalloproteinases inhibits hindbrain expansion. Transplanting dorsal hindbrain
cells to the spinal cord can locally create an expanded brain-like morphology
in some cases. Our findings raise questions in vertebrate head evolution and suggest
a general role of mechanical pre-patterning in sculpting epithelial tubes.
acknowledgement: 'We thank A. Dimitracopoulos, K. Kawaguchi, J. Vidigueira, B. Baum,
I. McLaren, D. St Johnston, and members of the Buckley, Scarpa, Steventon, Kawaguchi,
and Xiong labs for technical assistance and constructive feedback. We thank Ryan
Greenhalgh for methods developed to obtain fluidity values from AFM data. We thank
Nicola Lawrence, Alex Sossick, and Sargon Gross-Thebing from the Gurdon Institute
Imaging Facility for microscopy support. Funding: this work was supported by a Wellcome
Trust/Royal Society Sir Henry Dale Fellowship (215439/Z/19/Z) and UKRI-EPSRC Frontier
Research Grant (EP/X023761/1, originally selected as an ERC Starting Grant) to F.X.;
an ERC Consolidator Grant (772426), ERC Synergy Grant 101118729 UNFOLD, and Alexander
von Humboldt Professorship ( Alexander von Humboldt Foundation) to K.F.; and an
ERC Starting Grant (851288) to E.H.'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Susannah B.P.
full_name: Mclaren, Susannah B.P.
last_name: Mclaren
- first_name: Shi-lei
full_name: Xue, Shi-lei
id: 31D2C804-F248-11E8-B48F-1D18A9856A87
last_name: Xue
- first_name: Siyuan
full_name: Ding, Siyuan
last_name: Ding
- first_name: Alexander K.
full_name: Winkel, Alexander K.
last_name: Winkel
- first_name: Oscar
full_name: Baldwin, Oscar
last_name: Baldwin
- first_name: Shreya
full_name: Dwarakacherla, Shreya
last_name: Dwarakacherla
- first_name: Kristian
full_name: Franze, Kristian
last_name: Franze
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Fengzhu
full_name: Xiong, Fengzhu
last_name: Xiong
citation:
ama: Mclaren SBP, Xue S, Ding S, et al. Differential tissue deformability underlies
fluid pressure-driven shape divergence of the avian embryonic brain and spinal
cord. Developmental Cell. 2025;60(17):2237-2247.e4. doi:10.1016/j.devcel.2025.04.010
apa: Mclaren, S. B. P., Xue, S., Ding, S., Winkel, A. K., Baldwin, O., Dwarakacherla,
S., … Xiong, F. (2025). Differential tissue deformability underlies fluid pressure-driven
shape divergence of the avian embryonic brain and spinal cord. Developmental
Cell. Elsevier. https://doi.org/10.1016/j.devcel.2025.04.010
chicago: Mclaren, Susannah B.P., Shi-lei Xue, Siyuan Ding, Alexander K. Winkel,
Oscar Baldwin, Shreya Dwarakacherla, Kristian Franze, Edouard B Hannezo, and Fengzhu
Xiong. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape
Divergence of the Avian Embryonic Brain and Spinal Cord.” Developmental Cell.
Elsevier, 2025. https://doi.org/10.1016/j.devcel.2025.04.010.
ieee: S. B. P. Mclaren et al., “Differential tissue deformability underlies
fluid pressure-driven shape divergence of the avian embryonic brain and spinal
cord,” Developmental Cell, vol. 60, no. 17. Elsevier, p. 2237–2247.e4,
2025.
ista: Mclaren SBP, Xue S, Ding S, Winkel AK, Baldwin O, Dwarakacherla S, Franze
K, Hannezo EB, Xiong F. 2025. Differential tissue deformability underlies fluid
pressure-driven shape divergence of the avian embryonic brain and spinal cord.
Developmental Cell. 60(17), 2237–2247.e4.
mla: Mclaren, Susannah B. P., et al. “Differential Tissue Deformability Underlies
Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal
Cord.” Developmental Cell, vol. 60, no. 17, Elsevier, 2025, p. 2237–2247.e4,
doi:10.1016/j.devcel.2025.04.010.
short: S.B.P. Mclaren, S. Xue, S. Ding, A.K. Winkel, O. Baldwin, S. Dwarakacherla,
K. Franze, E.B. Hannezo, F. Xiong, Developmental Cell 60 (2025) 2237–2247.e4.
date_created: 2025-05-18T22:02:50Z
date_published: 2025-09-08T00:00:00Z
date_updated: 2025-12-29T14:58:14Z
day: '08'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1016/j.devcel.2025.04.010
ec_funded: 1
external_id:
isi:
- '001570502100005'
pmid:
- '40347948'
file:
- access_level: open_access
checksum: 1ca6f0822c1cbd430686d5e2a4f96401
content_type: application/pdf
creator: dernst
date_created: 2025-12-29T13:45:05Z
date_updated: 2025-12-29T13:45:05Z
file_id: '20872'
file_name: 2025_DevelopmentalCell_McLaren.pdf
file_size: 12564806
relation: main_file
success: 1
file_date_updated: 2025-12-29T13:45:05Z
has_accepted_license: '1'
intvolume: ' 60'
isi: 1
issue: '17'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 2237-2247.e4
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Developmental Cell
publication_identifier:
eissn:
- 1878-1551
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Differential tissue deformability underlies fluid pressure-driven shape divergence
of the avian embryonic brain and spinal cord
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 60
year: '2025'
...
---
APC_amount: 3642,79 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20080'
abstract:
- lang: eng
text: "Introduction: Acid-growth theory has been postulated in the 70s to explain
the rapid elongation of plant cells in response to the hormone auxin. More recently,
it has been demonstrated that activation of the proton ATPs pump (H+-ATPs) promoting
acidification of the apoplast is the principal mechanism by which auxin and other
hormones such as brassinosteroids (BR) induce cell elongation. Despite these advances,
the impact of this acidification on the mechanical properties of the cell wall
remained largely unexplored.\r\n\r\nMethods: Here, we use elongation assays of
Arabidopsis thaliana hypocotyls and Atomic Force Microscopy (AFM) to correlate
hormone-induced tissue elongation and local changes in cell wall mechanical properties.
Furthermore, employing transgenic lines over-expressing Pectin Methyl Esterase
(PME), along with calcium chelators, we investigate the effect of pectin modification
in hormone-driven cell elongation.\r\n\r\nResults: We demonstrate that acidification
of apoplast is necessary and sufficient to induce cell elongation through promoting
cell wall softening. Moreover, we show that enhanced PME activity can induce both
cell wall softening or stiffening in extracellular calcium dependent-manner and
that tight control of PME activity is required for proper hypocotyl elongation.\r\n\r\nDiscussion:
Our results confirm a dual role of PME in plant cell elongation. However, further
investigation is needed to assess the status of pectin following short- or long-term
PME treatments in order to determine if pectin methyl-esterification might promote
its degradation as well as the role of PME inhibitors upon PME induction."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: E-Lib
acknowledgement: "The author(s) declare that financial support was received for the
research and/or publication of this article. This work was supported by grants from
the European Research Council (Starting Independent Research Grant ERC-2007-Stg-
207362-HCPO to EB) and MG was recipient of an IST Interdisciplinary project (IC1022IPC03).\r\nWe
acknowledge Jaume F. Martı́nez Garcı́a for phyAphyB mutant seeds. We acknowledge
CF Nanobiotechnology of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127).
We gratefully acknowledge support by the Scientific Service Units at ISTA, including
the Imaging and Optics and Lab Support facilities and Library. We thank Stefan Riegler
for the efforts to establish immunodetection method."
article_number: '1612366'
article_processing_charge: Yes
article_type: original
author:
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Nikola
full_name: Zarevski, Nikola
id: 18e95355-e05a-11ea-a9c0-8fba1b89e83a
last_name: Zarevski
- first_name: Jan
full_name: Pribyl, Jan
last_name: Pribyl
- first_name: Petr
full_name: Skládal, Petr
last_name: Skládal
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Gallemi M, Montesinos López JC, Zarevski N, et al. Dual role of pectin methyl
esterase activity in the regulation of plant cell wall biophysical properties.
Frontiers in Plant Science. 2025;16. doi:10.3389/fpls.2025.1612366
apa: Gallemi, M., Montesinos López, J. C., Zarevski, N., Pribyl, J., Skládal, P.,
Hannezo, E. B., & Benková, E. (2025). Dual role of pectin methyl esterase
activity in the regulation of plant cell wall biophysical properties. Frontiers
in Plant Science. Frontiers Media. https://doi.org/10.3389/fpls.2025.1612366
chicago: Gallemi, Marçal, Juan C Montesinos López, Nikola Zarevski, Jan Pribyl,
Petr Skládal, Edouard B Hannezo, and Eva Benková. “Dual Role of Pectin Methyl
Esterase Activity in the Regulation of Plant Cell Wall Biophysical Properties.”
Frontiers in Plant Science. Frontiers Media, 2025. https://doi.org/10.3389/fpls.2025.1612366.
ieee: M. Gallemi et al., “Dual role of pectin methyl esterase activity in
the regulation of plant cell wall biophysical properties,” Frontiers in Plant
Science, vol. 16. Frontiers Media, 2025.
ista: Gallemi M, Montesinos López JC, Zarevski N, Pribyl J, Skládal P, Hannezo EB,
Benková E. 2025. Dual role of pectin methyl esterase activity in the regulation
of plant cell wall biophysical properties. Frontiers in Plant Science. 16, 1612366.
mla: Gallemi, Marçal, et al. “Dual Role of Pectin Methyl Esterase Activity in the
Regulation of Plant Cell Wall Biophysical Properties.” Frontiers in Plant Science,
vol. 16, 1612366, Frontiers Media, 2025, doi:10.3389/fpls.2025.1612366.
short: M. Gallemi, J.C. Montesinos López, N. Zarevski, J. Pribyl, P. Skládal, E.B.
Hannezo, E. Benková, Frontiers in Plant Science 16 (2025).
corr_author: '1'
date_created: 2025-07-27T22:01:26Z
date_published: 2025-07-04T00:00:00Z
date_updated: 2026-05-20T07:53:03Z
day: '04'
ddc:
- '580'
department:
- _id: EdHa
- _id: EvBe
- _id: CaGu
doi: 10.3389/fpls.2025.1612366
ec_funded: 1
external_id:
isi:
- '001530690900001'
pmid:
- '40688689'
file:
- access_level: open_access
checksum: 9e6b8b53ba56d4a24a9bd91cf6d2dc58
content_type: application/pdf
creator: dernst
date_created: 2025-07-31T07:28:54Z
date_updated: 2025-07-31T07:28:54Z
file_id: '20093'
file_name: 2025_FrontiersPlantSc_Gallemi.pdf
file_size: 3665187
relation: main_file
success: 1
file_date_updated: 2025-07-31T07:28:54Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
- _id: B67AFEDC-15C9-11EA-A837-991A96BB2854
name: IST Austria Open Access Fund
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664-462X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dual role of pectin methyl esterase activity in the regulation of plant cell
wall biophysical properties
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2025'
...
---
APC_amount: 5766,07 EUR
OA_place: publisher
OA_type: hybrid
PlanS_conform: '1'
_id: '20289'
abstract:
- lang: eng
text: Cell and tissue movement in development, cancer invasion, and immune response
relies on chemical or mechanical guidance cues. In many systems, this behavior
is locally directed by self-generated signaling gradients rather than long-range,
prepatterned cues. However, how heterogeneous mixtures of cells interact nonreciprocally
and navigate through self-generated gradients remains largely unexplored. Here,
we introduce a theoretical framework for the self-organized chemotaxis of heterogeneous
cell populations. We find that the relative chemotactic sensitivities of different
cell populations control their long-time coupling and comigration dynamics, with
boundary conditions such as external cell and attractant reservoirs substantially
influencing the migration patterns. Our model predicts an optimal parameter regime
that enables robust and colocalized migration. We test our theoretical predictions
with in vitro experiments demonstrating the comigration of distinct immune cell
populations, and quantitatively reproduce observed migration patterns under wild-type
and perturbed conditions. Interestingly, immune cell comigration occurs close
to the predicted optimal regime. Finally, we incorporate mechanical interactions
into our framework, revealing a nontrivial interplay between chemotactic and mechanical
nonreciprocity in driving collective migration. Together, our findings suggest
that self-generated chemotaxis is a robust strategy for the navigation of mixed
cell populations.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
- _id: LifeSc
- _id: NanoFab
acknowledgement: We thank all members of the M.S. and E.H. groups for stimulating
discussions.We thank the Imaging and Optics facility, the Pre-clinical and Lab Support
facility of the Institute of Science and Technology Austria for their excellent
support and provided resources for the experimental research. In particular, we
thank Jack Merrin from the Nanofabrication facility who generated the microfabricated
channel used in this study. This work received funding fromt he European Research
Council under the European Union’s Horizon 2020 research and innovation program
(grant agreement No. 851288 to E.H.). M.C.U.is funded by a University of Sheffield
Strategic Research Fellowship in the Physics of Life and Quantitative Biology.
article_number: e2504064122
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Mehmet C
full_name: Ucar, Mehmet C
id: 50B2A802-6007-11E9-A42B-EB23E6697425
last_name: Ucar
orcid: 0000-0003-0506-4217
- first_name: Alsberga
full_name: Zane, Alsberga
id: 60f7509a-f652-11ea-9d86-b963d6490d7c
last_name: Zane
orcid: 0009-0003-0415-7603
- first_name: Jonna H
full_name: Alanko, Jonna H
id: 2CC12E8C-F248-11E8-B48F-1D18A9856A87
last_name: Alanko
orcid: 0000-0002-7698-3061
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. Self-generated chemotaxis
of mixed cell populations. Proceedings of the National Academy of Sciences.
2025;122(34). doi:10.1073/pnas.2504064122
apa: Ucar, M. C., Zane, A., Alanko, J. H., Sixt, M. K., & Hannezo, E. B. (2025).
Self-generated chemotaxis of mixed cell populations. Proceedings of the National
Academy of Sciences. National Academy of Sciences. https://doi.org/10.1073/pnas.2504064122
chicago: Ucar, Mehmet C, Alsberga Zane, Jonna H Alanko, Michael K Sixt, and Edouard
B Hannezo. “Self-Generated Chemotaxis of Mixed Cell Populations.” Proceedings
of the National Academy of Sciences. National Academy of Sciences, 2025. https://doi.org/10.1073/pnas.2504064122.
ieee: M. C. Ucar, A. Zane, J. H. Alanko, M. K. Sixt, and E. B. Hannezo, “Self-generated
chemotaxis of mixed cell populations,” Proceedings of the National Academy
of Sciences, vol. 122, no. 34. National Academy of Sciences, 2025.
ista: Ucar MC, Zane A, Alanko JH, Sixt MK, Hannezo EB. 2025. Self-generated chemotaxis
of mixed cell populations. Proceedings of the National Academy of Sciences. 122(34),
e2504064122.
mla: Ucar, Mehmet C., et al. “Self-Generated Chemotaxis of Mixed Cell Populations.”
Proceedings of the National Academy of Sciences, vol. 122, no. 34, e2504064122,
National Academy of Sciences, 2025, doi:10.1073/pnas.2504064122.
short: M.C. Ucar, A. Zane, J.H. Alanko, M.K. Sixt, E.B. Hannezo, Proceedings of
the National Academy of Sciences 122 (2025).
corr_author: '1'
date_created: 2025-09-07T22:01:32Z
date_published: 2025-08-26T00:00:00Z
date_updated: 2026-05-20T08:59:54Z
day: '26'
ddc:
- '570'
department:
- _id: EdHa
- _id: MiSi
doi: 10.1073/pnas.2504064122
ec_funded: 1
external_id:
isi:
- '001562181600001'
pmid:
- '40838890'
file:
- access_level: open_access
checksum: b36abd92673b6d76376fc9434bad52cc
content_type: application/pdf
creator: dernst
date_created: 2025-09-08T07:23:29Z
date_updated: 2025-09-08T07:23:29Z
file_id: '20307'
file_name: 2025_PNAS_Ucar.pdf
file_size: 16069140
relation: main_file
success: 1
file_date_updated: 2025-09-08T07:23:29Z
has_accepted_license: '1'
intvolume: ' 122'
isi: 1
issue: '34'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- relation: software
url: https://github.com/mehmetcanucar/Self-generated-chemotaxis
scopus_import: '1'
status: public
title: Self-generated chemotaxis of mixed cell populations
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 122
year: '2025'
...
---
APC_amount: 7068 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '20424'
abstract:
- lang: eng
text: Homeostasis relies on a precise balance of fate choices between renewal and
differentiation. Although progress has been done to characterize the dynamics
of single-cell fate choices, their underlying mechanistic basis often remains
unclear. Concentrating on skin epidermis as a paradigm for multilayered tissues
with complex fate choices, we develop a 3D vertex-based model with proliferation
in the basal layer, showing that mechanical competition for space naturally gives
rise to homeostasis and neutral drift dynamics that are seen experimentally. We
then explore the effect of introducing mechanical heterogeneities between cellular
subpopulations. We uncover that relatively small tension heterogeneities, reflected
by distinct morphological changes in single-cell shapes, can be sufficient to
heavily tilt cellular dynamics towards exponential growth. We thus derive a master
relationship between cell shape and long-term clonal dynamics, which we validated
during basal cell carcinoma initiation in mouse epidermis. Altogether, we propose
a theoretical framework to link mechanical forces, quantitative cellular morphologies
and cellular fate outcomes in complex tissues.
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank Alois Schlögl, Paula Sanematsu, Susana Moreno Flores, Bernat
Corominas-Murtra, Stefania Tavano, Gayathri Singharaju, and Hannezo group members
for helpful discussions, the Bioimaging facility at ISTA, as well as Matthias Merkel
and Lisa Manning for sharing the 3D Voronoi code. We also thank the Champalimaud
animal facility, Anna Pezzarossa and the Champalimaud ABBE platform for the help
with microscopy and image processing. This work was supported by EMBO (ALTF 522-2021),
a Fundação para a Ciência e Tecnologia grant to A.S.D. (PTDC/MED-ONC/5553/2020),
as well as the European Research Council (grant 851288 to EH). A.S.D., S.C., and
R.M.S. are supported by QuantOCancer Project Horizon European Union’s Horizon 2020
program (grant agreement No 810653).
article_number: '8440'
article_processing_charge: Yes
article_type: original
author:
- first_name: Preeti
full_name: Sahu, Preeti
id: 55BA52EE-A185-11EA-88FD-18AD3DDC885E
last_name: Sahu
- first_name: Sara
full_name: Monteiro-Ferreira, Sara
last_name: Monteiro-Ferreira
- first_name: Sara
full_name: Canato, Sara
last_name: Canato
- first_name: Raquel Maia
full_name: Soares, Raquel Maia
last_name: Soares
- first_name: Adriana
full_name: Sánchez-Danés, Adriana
last_name: Sánchez-Danés
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Sahu P, Monteiro-Ferreira S, Canato S, Soares RM, Sánchez-Danés A, Hannezo
EB. Mechanical control of cell fate decisions in the skin epidermis. Nature
Communications. 2025;16. doi:10.1038/s41467-025-62882-9
apa: Sahu, P., Monteiro-Ferreira, S., Canato, S., Soares, R. M., Sánchez-Danés,
A., & Hannezo, E. B. (2025). Mechanical control of cell fate decisions in
the skin epidermis. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-025-62882-9
chicago: Sahu, Preeti, Sara Monteiro-Ferreira, Sara Canato, Raquel Maia Soares,
Adriana Sánchez-Danés, and Edouard B Hannezo. “Mechanical Control of Cell Fate
Decisions in the Skin Epidermis.” Nature Communications. Springer Nature,
2025. https://doi.org/10.1038/s41467-025-62882-9.
ieee: P. Sahu, S. Monteiro-Ferreira, S. Canato, R. M. Soares, A. Sánchez-Danés,
and E. B. Hannezo, “Mechanical control of cell fate decisions in the skin epidermis,”
Nature Communications, vol. 16. Springer Nature, 2025.
ista: Sahu P, Monteiro-Ferreira S, Canato S, Soares RM, Sánchez-Danés A, Hannezo
EB. 2025. Mechanical control of cell fate decisions in the skin epidermis. Nature
Communications. 16, 8440.
mla: Sahu, Preeti, et al. “Mechanical Control of Cell Fate Decisions in the Skin
Epidermis.” Nature Communications, vol. 16, 8440, Springer Nature, 2025,
doi:10.1038/s41467-025-62882-9.
short: P. Sahu, S. Monteiro-Ferreira, S. Canato, R.M. Soares, A. Sánchez-Danés,
E.B. Hannezo, Nature Communications 16 (2025).
corr_author: '1'
date_created: 2025-10-05T22:01:34Z
date_published: 2025-09-26T00:00:00Z
date_updated: 2026-05-20T08:52:01Z
day: '26'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1038/s41467-025-62882-9
ec_funded: 1
external_id:
isi:
- '001582555200011'
pmid:
- '41006218'
file:
- access_level: open_access
checksum: d1656576883b23902545328e2d640234
content_type: application/pdf
creator: dernst
date_created: 2025-10-13T12:37:04Z
date_updated: 2025-10-13T12:37:04Z
file_id: '20464'
file_name: 2025_NatureComm_Sahu.pdf
file_size: 2816813
relation: main_file
success: 1
file_date_updated: 2025-10-13T12:37:04Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 628f3fb1-2b32-11ec-9570-83ce778803f7
grant_number: ALTF 522-2021
name: Biomechanics of stem cell fate determination
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanical control of cell fate decisions in the skin epidermis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2025'
...
---
OA_place: repository
OA_type: green
_id: '20431'
abstract:
- lang: eng
text: Haptotaxis is the process of directed cell migration along gradients of extracellular
matrix density and is central to morphogenesis, immune responses and cancer invasion.
It is commonly assumed that cells respond to these gradients by migrating directionally
towards the regions of highest ligand density. In contrast with this view, here
we show that cells exposed to micropatterned fibronectin gradients exhibit a wide
range of complex trajectories, including directed haptotactic migration up the
gradient but also linear oscillations and circles with extended periods of migration
down the gradient. To explain this behaviour, we developed a biophysical model
of haptotactic cell migration based on a coarse-grained molecular clutch model
coupled to persistent stochastic polarity dynamics. Although initial haptotactic
migration is explained by the differential friction at the front and back of the
cell, the observed complex trajectories over longer timescales arise from the
interplay between differential friction, persistence and physical confinement.
Overall, our study reveals that confinement and persistence modulate the ability
of cells to sense and respond to haptotactic cues and provides a framework for
understanding how cells navigate complex environments.
acknowledgement: We thank all the members of our groups for discussions and support.
We thank A. Menéndez, S. Usieto, M. Purciolas and E. Coderch for technical assistance.
We thank G. Charras (London Centre for Nanotechnology, UK) and M. Sheetz (Columbia
University, USA) for sharing cells used in this work. We thank J. Ivaska (University
of Turku, Finland) for sharing integrin α5-GFP DNA plasmid. We thank P. Guillamat
for technical advice and A. Labernardie for providing the microfluidic channels.
We thank M. Gómez-González for sharing the 2D traction microscopy algorithm. Finally,
we thank P. Guillamat, J. Abenza, G. Ceada, L. Faure, E. Dalaka, M. Matejčić, A.
Beedle, I. Granero, O. Baguer, A. Albajar and N. Chahare for discussions. This paper
was funded by the Generalitat de Catalunya (Grant Nos. AGAUR SGR-2017-01602 to X.T.
and 2021 SGR 00523 to R.S. and the CERCA Programme and ICREA Academia awards to
P.R.-C.), the Spanish Ministry for Science and Innovation MICCINN/FEDER (Grant Nos.
PID2021-128635NB-I00, MCIN/AEI/10.13039/501100011033 and ERDF-EU A way of making
Europe to X.T., PID2021-128674OB-I00 and CNS2022-135533 to R.S. and PID2019-110298GB-I00
to P.R.-C.), the European Research Council (Grant Nos. 101097753 to P.R.-C. and
Adv-883739 to X.T.), Fundació la Marató de TV3 (Project Award 201903-30-31-32 to
X.T.), the European Commission (Grant No. H2020-FETPROACT-01-2016-731957 to P.R.-C.
and X.T.) and La Caixa Foundation (Grant No. LCF/PR/HR20/52400004 to P.R.-C. and
X.T.). R.S. is a Serra-Hunter fellow. D.B.B. was supported by the NOMIS foundation
as a NOMIS fellow, by the European Molecular Biology Organization (Postdoctoral
Fellowship ALTF 343-2022) and by the Austrian Academy of Sciences through an APART-MINT
Fellowship. I.C.F. acknowledges support from the European Foundation for the Study
of Chronic Liver Failure. IBEC is recipient of a Severo Ochoa Award of Excellence
from MINECO.
article_processing_charge: No
article_type: original
author:
- first_name: Isabela Corina
full_name: Fortunato, Isabela Corina
last_name: Fortunato
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Steffen
full_name: Grosser, Steffen
last_name: Grosser
- first_name: Rohit
full_name: Nautiyal, Rohit
last_name: Nautiyal
- first_name: Leone
full_name: Rossetti, Leone
last_name: Rossetti
- first_name: Miquel
full_name: Bosch-Padrós, Miquel
last_name: Bosch-Padrós
- first_name: Jonel
full_name: Trebicka, Jonel
last_name: Trebicka
- first_name: Pere
full_name: Roca-Cusachs, Pere
last_name: Roca-Cusachs
- first_name: Raimon
full_name: Sunyer, Raimon
last_name: Sunyer
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Xavier
full_name: Trepat, Xavier
last_name: Trepat
citation:
ama: Fortunato IC, Brückner D, Grosser S, et al. Single-cell migration along and
against confined haptotactic gradients. Nature Physics. 2025;21:1638-1647.
doi:10.1038/s41567-025-03015-3
apa: Fortunato, I. C., Brückner, D., Grosser, S., Nautiyal, R., Rossetti, L., Bosch-Padrós,
M., … Trepat, X. (2025). Single-cell migration along and against confined haptotactic
gradients. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-025-03015-3
chicago: Fortunato, Isabela Corina, David Brückner, Steffen Grosser, Rohit Nautiyal,
Leone Rossetti, Miquel Bosch-Padrós, Jonel Trebicka, et al. “Single-Cell Migration
along and against Confined Haptotactic Gradients.” Nature Physics. Springer
Nature, 2025. https://doi.org/10.1038/s41567-025-03015-3.
ieee: I. C. Fortunato et al., “Single-cell migration along and against confined
haptotactic gradients,” Nature Physics, vol. 21. Springer Nature, pp. 1638–1647,
2025.
ista: Fortunato IC, Brückner D, Grosser S, Nautiyal R, Rossetti L, Bosch-Padrós
M, Trebicka J, Roca-Cusachs P, Sunyer R, Hannezo EB, Trepat X. 2025. Single-cell
migration along and against confined haptotactic gradients. Nature Physics. 21,
1638–1647.
mla: Fortunato, Isabela Corina, et al. “Single-Cell Migration along and against
Confined Haptotactic Gradients.” Nature Physics, vol. 21, Springer Nature,
2025, pp. 1638–47, doi:10.1038/s41567-025-03015-3.
short: I.C. Fortunato, D. Brückner, S. Grosser, R. Nautiyal, L. Rossetti, M. Bosch-Padrós,
J. Trebicka, P. Roca-Cusachs, R. Sunyer, E.B. Hannezo, X. Trepat, Nature Physics
21 (2025) 1638–1647.
corr_author: '1'
date_created: 2025-10-05T22:01:36Z
date_published: 2025-10-01T00:00:00Z
date_updated: 2026-01-05T14:26:28Z
day: '01'
department:
- _id: EdHa
doi: 10.1038/s41567-025-03015-3
external_id:
isi:
- '001581659900001'
intvolume: ' 21'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2024.12.02.626413
month: '10'
oa: 1
oa_version: Preprint
page: 1638-1647
project:
- _id: 34e2a5b5-11ca-11ed-8bc3-b2265616ef0b
grant_number: ALTF 343-2022
name: A mechano-chemical theory for stem cell fate decisions in organoid development
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Single-cell migration along and against confined haptotactic gradients
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 21
year: '2025'
...
---
OA_place: repository
_id: '20465'
abstract:
- lang: eng
text: For tissues to spread, they must be deformable while maintaining their structural
integrity. How these opposing requirements are balanced within spreading tissues
is not yet well understood. Here, we show that keratin intermediate filaments
function in epithelial spreading by adapting tissue mechanical resilience to the
stresses arising in the tissue during the spreading process. By analysing the
expansion of the enveloping cell layer (EVL) over the large yolk cell in early
zebrafish embryos in vivo, we found that keratin network maturation in EVL cells
is promoted by stresses building up within the spreading tissue. Through genetic
interference and tissue rheology experiments, complemented by a vertex model with
mechanochemical feedback, we demonstrate that stress-induced keratin network maturation
in the EVL increases tissue viscosity, which is essential for preventing tissue
rupture. Interestingly, keratins are also required in the yolk cell for mechanosensitive
actomyosin network contraction and flow, the force-generating processes pulling
the EVL. These dual mechanosensitive functions of keratins enable a balance between
pulling force production in the yolk cell and the mechanical resilience of the
EVL against stresses generated by these pulling forces, thereby ensuring uniform
and robust tissue spreading.
article_processing_charge: No
author:
- first_name: Suyash
full_name: Naik, Suyash
id: 2C0B105C-F248-11E8-B48F-1D18A9856A87
last_name: Naik
orcid: 0000-0001-8421-5508
- first_name: Yann-Edwin
full_name: Keta, Yann-Edwin
last_name: Keta
- first_name: Kornelija
full_name: Pranjic-Ferscha, Kornelija
id: 4362B3C2-F248-11E8-B48F-1D18A9856A87
last_name: Pranjic-Ferscha
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Silke
full_name: Henkes, Silke
last_name: Henkes
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ.
Keratins coordinate tissue spreading by balancing spreading forces with tissue
material properties. bioRxiv. doi:10.1101/2025.02.14.638262
apa: Naik, S., Keta, Y.-E., Pranjic-Ferscha, K., Hannezo, E. B., Henkes, S., &
Heisenberg, C.-P. J. (n.d.). Keratins coordinate tissue spreading by balancing
spreading forces with tissue material properties. bioRxiv. Cold Spring
Harbor Laboratory. https://doi.org/10.1101/2025.02.14.638262
chicago: Naik, Suyash, Yann-Edwin Keta, Kornelija Pranjic-Ferscha, Edouard B Hannezo,
Silke Henkes, and Carl-Philipp J Heisenberg. “Keratins Coordinate Tissue Spreading
by Balancing Spreading Forces with Tissue Material Properties.” BioRxiv.
Cold Spring Harbor Laboratory, n.d. https://doi.org/10.1101/2025.02.14.638262.
ieee: S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E. B. Hannezo, S. Henkes, and C.-P.
J. Heisenberg, “Keratins coordinate tissue spreading by balancing spreading forces
with tissue material properties,” bioRxiv. Cold Spring Harbor Laboratory.
ista: Naik S, Keta Y-E, Pranjic-Ferscha K, Hannezo EB, Henkes S, Heisenberg C-PJ.
Keratins coordinate tissue spreading by balancing spreading forces with tissue
material properties. bioRxiv, 10.1101/2025.02.14.638262.
mla: Naik, Suyash, et al. “Keratins Coordinate Tissue Spreading by Balancing Spreading
Forces with Tissue Material Properties.” BioRxiv, Cold Spring Harbor Laboratory,
doi:10.1101/2025.02.14.638262.
short: S. Naik, Y.-E. Keta, K. Pranjic-Ferscha, E.B. Hannezo, S. Henkes, C.-P.J.
Heisenberg, BioRxiv (n.d.).
corr_author: '1'
date_created: 2025-10-14T07:25:27Z
date_published: 2025-02-17T00:00:00Z
date_updated: 2026-04-07T11:58:57Z
day: '17'
department:
- _id: CaHe
- _id: EdHa
doi: 10.1101/2025.02.14.638262
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nd/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2025.02.14.638262
month: '02'
oa: 1
oa_version: Preprint
publication: bioRxiv
publication_status: draft
publisher: Cold Spring Harbor Laboratory
related_material:
record:
- id: '20441'
relation: dissertation_contains
status: public
status: public
title: Keratins coordinate tissue spreading by balancing spreading forces with tissue
material properties
tmp:
image: /image/cc_by_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nd/4.0/legalcode
name: Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0)
short: CC BY-ND (4.0)
type: preprint
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2025'
...
---
APC_amount: 4695,11 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20708'
abstract:
- lang: eng
text: In equilibrium, the physical properties of matter are set by the interactions
between the constituents. In contrast, the energy input of the individual components
controls the behavior of synthetic or living active matter. Great progress has
been made in understanding the emergent phenomena in active fluids, though their
inability to resist shear forces hinders their practical use. This motivates the
exploration of active solids as shape-shifting materials, yet, we lack controlled
synthetic systems to devise active solids with unconventional properties. Here
we build active elastic beams from dozens of active colloids and unveil complex
emergent behaviors such as self-oscillations or persistent rotations. Developing
tensile tests at the microscale, we show that the active beams are ultrasoft materials,
with large (nonequilibrium) fluctuations. Combining experiments, theory, and stochastic
inference, we show that the dynamics of the active beams can be mapped on different
phase transitions which are tuned by boundary conditions. More quantitatively,
we assess all relevant parameters by independent measurements or first-principles
calculations, and find that our theoretical description agrees with the experimental
observations. Our results demonstrate that the simple addition of activity to
an elastic beam unveils novel physics and can inspire design strategies for active
solids and functional microscopic machines.
acknowledgement: The authors thank Andela Saric, Christoph Zechner, and Paul Robin
for helpful discussions. J. P. acknowledges support by ERC grant (VULCAN, 101086998)
and U.S. ARO under Award No. W911NF2310008. Y. I. L. acknowledges funding from the
European Union’s Horizon 2020 research and innovation programme under the Marie
Skłodowska-Curie Grant Agreement No. 101034413.
article_number: '041017'
article_processing_charge: Yes
article_type: original
arxiv: 1
author:
- first_name: Quentin
full_name: Martinet, Quentin
id: b37485a8-d343-11eb-a0e9-df8c484ef8ab
last_name: Martinet
orcid: 0000-0002-2916-6632
- first_name: Yuting I
full_name: Li, Yuting I
id: ee7a5ca8-8b71-11ed-b662-b3341c05b7eb
last_name: Li
- first_name: A.
full_name: Aubret, A.
last_name: Aubret
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jérémie A
full_name: Palacci, Jérémie A
id: 8fb92548-2b22-11eb-b7c1-a3f0d08d7c7d
last_name: Palacci
orcid: 0000-0002-7253-9465
citation:
ama: Martinet Q, Li YI, Aubret A, Hannezo EB, Palacci JA. Emergent dynamics of active
elastic microbeams. Physical Review X. 2025;15(4). doi:10.1103/rjk2-q2wh
apa: Martinet, Q., Li, Y. I., Aubret, A., Hannezo, E. B., & Palacci, J. A. (2025).
Emergent dynamics of active elastic microbeams. Physical Review X. American
Physical Society. https://doi.org/10.1103/rjk2-q2wh
chicago: Martinet, Quentin, Yuting I Li, A. Aubret, Edouard B Hannezo, and Jérémie
A Palacci. “Emergent Dynamics of Active Elastic Microbeams.” Physical Review
X. American Physical Society, 2025. https://doi.org/10.1103/rjk2-q2wh.
ieee: Q. Martinet, Y. I. Li, A. Aubret, E. B. Hannezo, and J. A. Palacci, “Emergent
dynamics of active elastic microbeams,” Physical Review X, vol. 15, no.
4. American Physical Society, 2025.
ista: Martinet Q, Li YI, Aubret A, Hannezo EB, Palacci JA. 2025. Emergent dynamics
of active elastic microbeams. Physical Review X. 15(4), 041017.
mla: Martinet, Quentin, et al. “Emergent Dynamics of Active Elastic Microbeams.”
Physical Review X, vol. 15, no. 4, 041017, American Physical Society, 2025,
doi:10.1103/rjk2-q2wh.
short: Q. Martinet, Y.I. Li, A. Aubret, E.B. Hannezo, J.A. Palacci, Physical Review
X 15 (2025).
corr_author: '1'
date_created: 2025-11-30T23:02:08Z
date_published: 2025-10-31T00:00:00Z
date_updated: 2026-05-20T08:58:06Z
day: '31'
ddc:
- '530'
department:
- _id: EdHa
- _id: JePa
doi: 10.1103/rjk2-q2wh
ec_funded: 1
external_id:
arxiv:
- '2508.20642'
file:
- access_level: open_access
checksum: bb64ea9f2c400205fd89e9bdd15cc850
content_type: application/pdf
creator: dernst
date_created: 2025-12-01T07:30:00Z
date_updated: 2025-12-01T07:30:00Z
file_id: '20714'
file_name: 2025_PhysicalReviewX_Martinet.pdf
file_size: 5902259
relation: main_file
success: 1
file_date_updated: 2025-12-01T07:30:00Z
has_accepted_license: '1'
intvolume: ' 15'
issue: '4'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: bdac72da-d553-11ed-ba76-eae56e802b74
grant_number: '101086998'
name: 'VULCAN: matter, powered from within'
- _id: fc2ed2f7-9c52-11eb-aca3-c01059dda49c
call_identifier: H2020
grant_number: '101034413'
name: 'IST-BRIDGE: International postdoctoral program'
publication: Physical Review X
publication_identifier:
eissn:
- 2160-3308
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Emergent dynamics of active elastic microbeams
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2025'
...
---
OA_place: publisher
OA_type: hybrid
_id: '18807'
abstract:
- lang: eng
text: Developing tissues interpret dynamic changes in morphogen activity to generate
cell type diversity. To quantitatively study bone morphogenetic protein (BMP)
signaling dynamics in the mouse neural tube, we developed an embryonic stem cell
differentiation system tailored for growing tissues. Differentiating cells form
striking self-organized patterns of dorsal neural tube cell types driven by sequential
phases of BMP signaling that are observed both in vitro and in vivo. Data-driven
biophysical modeling showed that these dynamics result from coupling fast negative
feedback with slow positive regulation of signaling by the specification of an
endogenous BMP source. Thus, in contrast to relays that propagate morphogen signaling
in space, we identify a BMP signaling relay that operates in time. This mechanism
allows for a rapid initial concentration-sensitive response that is robustly terminated,
thereby regulating balanced sequential cell type generation. Our study provides
an experimental and theoretical framework to understand how signaling dynamics
are exploited in developing tissues.
acknowledgement: We thank A. Miller and N. Papalopulu for reagents and J. Briscoe
for comments on the manuscript. Work in the A.K. lab is supported by ISTA; the European
Research Council under Horizon Europe, grant 101044579; and the Austrian Science
Fund (FWF), grant https://doi.org/10.55776/F78. S.L. is supported by Gesellschaft
für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. D.B.B. was
supported by the NOMIS foundation as a NOMIS Fellow and by an EMBO Postdoctoral
Fellowship (ALTF 343-2022).
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Stefanie
full_name: Rus, Stefanie
id: 4D9EC9B6-F248-11E8-B48F-1D18A9856A87
last_name: Rus
orcid: 0000-0001-8703-1093
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Thomas
full_name: Minchington, Thomas
id: 7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f
last_name: Minchington
- first_name: Martina
full_name: Greunz, Martina
id: 48A59534-F248-11E8-B48F-1D18A9856A87
last_name: Greunz
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Anna
full_name: Kicheva, Anna
id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
last_name: Kicheva
orcid: 0000-0003-4509-4998
citation:
ama: Rus S, Brückner D, Minchington T, et al. Self-organized pattern formation in
the developing mouse neural tube by a temporal relay of BMP signaling. Developmental
Cell. 2025;60(4):567-580. doi:10.1016/j.devcel.2024.10.024
apa: Rus, S., Brückner, D., Minchington, T., Greunz, M., Merrin, J., Hannezo, E.
B., & Kicheva, A. (2025). Self-organized pattern formation in the developing
mouse neural tube by a temporal relay of BMP signaling. Developmental Cell.
Elsevier. https://doi.org/10.1016/j.devcel.2024.10.024
chicago: Rus, Stefanie, David Brückner, Thomas Minchington, Martina Greunz, Jack
Merrin, Edouard B Hannezo, and Anna Kicheva. “Self-Organized Pattern Formation
in the Developing Mouse Neural Tube by a Temporal Relay of BMP Signaling.” Developmental
Cell. Elsevier, 2025. https://doi.org/10.1016/j.devcel.2024.10.024.
ieee: S. Rus et al., “Self-organized pattern formation in the developing
mouse neural tube by a temporal relay of BMP signaling,” Developmental Cell,
vol. 60, no. 4. Elsevier, pp. 567–580, 2025.
ista: Rus S, Brückner D, Minchington T, Greunz M, Merrin J, Hannezo EB, Kicheva
A. 2025. Self-organized pattern formation in the developing mouse neural tube
by a temporal relay of BMP signaling. Developmental Cell. 60(4), 567–580.
mla: Rus, Stefanie, et al. “Self-Organized Pattern Formation in the Developing Mouse
Neural Tube by a Temporal Relay of BMP Signaling.” Developmental Cell,
vol. 60, no. 4, Elsevier, 2025, pp. 567–80, doi:10.1016/j.devcel.2024.10.024.
short: S. Rus, D. Brückner, T. Minchington, M. Greunz, J. Merrin, E.B. Hannezo,
A. Kicheva, Developmental Cell 60 (2025) 567–580.
corr_author: '1'
date_created: 2025-01-09T11:25:47Z
date_published: 2025-02-24T00:00:00Z
date_updated: 2026-06-27T22:30:31Z
day: '24'
ddc:
- '570'
department:
- _id: AnKi
- _id: EdHa
- _id: NanoFab
doi: 10.1016/j.devcel.2024.10.024
external_id:
isi:
- '001434279000001'
pmid:
- '39603235'
file:
- access_level: open_access
checksum: bb58db4a908a1f4aabe4004706154541
content_type: application/pdf
creator: dernst
date_created: 2025-04-16T10:54:07Z
date_updated: 2025-04-16T10:54:07Z
file_id: '19584'
file_name: 2025_DevelopmentalCell_Lehr.pdf
file_size: 6994499
relation: main_file
success: 1
file_date_updated: 2025-04-16T10:54:07Z
has_accepted_license: '1'
intvolume: ' 60'
isi: 1
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 567-580
pmid: 1
project:
- _id: bd7e737f-d553-11ed-ba76-d69ffb5ee3aa
grant_number: '101044579'
name: Mechanisms of tissue size regulation in spinal cord development
- _id: 059DF620-7A3F-11EA-A408-12923DDC885E
grant_number: F7802
name: Stem Cell Modulation in Neural Development and Regeneration/ P02-Morphogen
control of growth and pattern in the spinal cord
- _id: 9B9B39FA-BA93-11EA-9121-9846C619BF3A
grant_number: SC19-011
name: The regulatory logic of pattern formation in the vertebrate dorsal neural
tube
publication: Developmental Cell
publication_identifier:
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '19763'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Self-organized pattern formation in the developing mouse neural tube by a temporal
relay of BMP signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 60
year: '2025'
...
---
_id: '14795'
abstract:
- lang: eng
text: Metazoan development relies on the formation and remodeling of cell-cell contacts.
Dynamic reorganization of adhesion receptors and the actomyosin cell cortex in
space and time plays a central role in cell-cell contact formation and maturation.
Nevertheless, how this process is mechanistically achieved when new contacts are
formed remains unclear. Here, by building a biomimetic assay composed of progenitor
cells adhering to supported lipid bilayers functionalized with E-cadherin ectodomains,
we show that cortical F-actin flows, driven by the depletion of myosin-2 at the
cell contact center, mediate the dynamic reorganization of adhesion receptors
and cell cortex at the contact. E-cadherin-dependent downregulation of the small
GTPase RhoA at the forming contact leads to both a depletion of myosin-2 and a
decrease of F-actin at the contact center. At the contact rim, in contrast, myosin-2
becomes enriched by the retraction of bleb-like protrusions, resulting in a cortical
tension gradient from the contact rim to its center. This tension gradient, in
turn, triggers centrifugal F-actin flows, leading to further accumulation of F-actin
at the contact rim and the progressive redistribution of E-cadherin from the contact
center to the rim. Eventually, this combination of actomyosin downregulation and
flows at the contact determines the characteristic molecular organization, with
E-cadherin and F-actin accumulating at the contact rim, where they are needed
to mechanically link the contractile cortices of the adhering cells.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: "We are grateful to Edwin Munro for their feedback and help with
the single particle analysis. We thank members of the Heisenberg and Loose labs
for their help and feedback on the manuscript, notably Xin Tong for making the PCS2-mCherry-AHPH
plasmid. Finally, we thank the Aquatics and Imaging & Optics facilities of ISTA
for their continuous support, especially Yann Cesbron for assistance with the laser
cutter. This work was supported by an ERC\r\nAdvanced Grant (MECSPEC) to C.-P.H."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Feyza N
full_name: Arslan, Feyza N
id: 49DA7910-F248-11E8-B48F-1D18A9856A87
last_name: Arslan
orcid: 0000-0001-5809-9566
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. Adhesion-induced
cortical flows pattern E-cadherin-mediated cell contacts. Current Biology.
2024;34(1):171-182.e8. doi:10.1016/j.cub.2023.11.067
apa: Arslan, F. N., Hannezo, E. B., Merrin, J., Loose, M., & Heisenberg, C.-P.
J. (2024). Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts.
Current Biology. Elsevier. https://doi.org/10.1016/j.cub.2023.11.067
chicago: Arslan, Feyza N, Edouard B Hannezo, Jack Merrin, Martin Loose, and Carl-Philipp
J Heisenberg. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated Cell
Contacts.” Current Biology. Elsevier, 2024. https://doi.org/10.1016/j.cub.2023.11.067.
ieee: F. N. Arslan, E. B. Hannezo, J. Merrin, M. Loose, and C.-P. J. Heisenberg,
“Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts,” Current
Biology, vol. 34, no. 1. Elsevier, p. 171–182.e8, 2024.
ista: Arslan FN, Hannezo EB, Merrin J, Loose M, Heisenberg C-PJ. 2024. Adhesion-induced
cortical flows pattern E-cadherin-mediated cell contacts. Current Biology. 34(1),
171–182.e8.
mla: Arslan, Feyza N., et al. “Adhesion-Induced Cortical Flows Pattern E-Cadherin-Mediated
Cell Contacts.” Current Biology, vol. 34, no. 1, Elsevier, 2024, p. 171–182.e8,
doi:10.1016/j.cub.2023.11.067.
short: F.N. Arslan, E.B. Hannezo, J. Merrin, M. Loose, C.-P.J. Heisenberg, Current
Biology 34 (2024) 171–182.e8.
corr_author: '1'
date_created: 2024-01-14T23:00:56Z
date_published: 2024-01-08T00:00:00Z
date_updated: 2025-09-04T11:39:10Z
day: '08'
ddc:
- '570'
department:
- _id: CaHe
- _id: EdHa
- _id: MaLo
- _id: NanoFab
doi: 10.1016/j.cub.2023.11.067
ec_funded: 1
external_id:
isi:
- '001154500400001'
pmid:
- '38134934'
file:
- access_level: open_access
checksum: 51220b76d72a614208f84bdbfbaf9b72
content_type: application/pdf
creator: dernst
date_created: 2024-01-16T10:53:31Z
date_updated: 2024-01-16T10:53:31Z
file_id: '14813'
file_name: 2024_CurrentBiology_Arslan.pdf
file_size: 5183861
relation: main_file
success: 1
file_date_updated: 2024-01-16T10:53:31Z
has_accepted_license: '1'
intvolume: ' 34'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 171-182.e8
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
publication: Current Biology
publication_identifier:
eissn:
- 1879-0445
issn:
- 0960-9822
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adhesion-induced cortical flows pattern E-cadherin-mediated cell contacts
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 34
year: '2024'
...
---
_id: '17104'
abstract:
- lang: eng
text: 'The homeostasis of epithelial tissue relies on a balance between the self-renewal
of stem cell populations, cellular differentiation, and loss. Although this balance
needs to be tightly regulated to avoid pathologies, such as tumor growth, the
regulatory mechanisms, both cell-intrinsic and collective, which ensure tissue
steady-state are still poorly understood. Here, we develop a computational model
that incorporates basic assumptions of stem cell renewal into distinct populations
and mechanical interactions between cells. We find that the model generates unexpected
dynamic features: stem cells repel each other in the bulk tissue and are thus
found rather isolated, as in a number of in vivo contexts. By mapping the system
onto a gas of passive Brownian particles with effective repulsive interactions,
that arise from the generated flows of differentiated cells, we show that we can
quantitatively describe such stem cell distribution in tissues. The interaction
potential between a pair of stem cells decays exponentially with a characteristic
length that spans several cell sizes, corresponding to the volume of cells generated
per stem cell division. Our findings may help understanding the dynamics of normal
and cancerous epithelial tissues.'
acknowledgement: JE and JK gratefully acknowledge financial support from the Initiative
and Networking Fund (IVF) via the grant number ERC-RA-004. Simulations were performed
with computing resources granted by RWTH Aachen University under project ‘rwth0475’.
article_number: '097'
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Johannes C.
full_name: Krämer, Johannes C.
last_name: Krämer
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Gerhard
full_name: Gompper, Gerhard
last_name: Gompper
- first_name: Jens
full_name: Elgeti, Jens
last_name: Elgeti
citation:
ama: Krämer JC, Hannezo EB, Gompper G, Elgeti J. Mechanically-driven stem cell separation
in tissues caused by proliferating daughter cells. SciPost Physics. 2024;16(4).
doi:10.21468/scipostphys.16.4.097
apa: Krämer, J. C., Hannezo, E. B., Gompper, G., & Elgeti, J. (2024). Mechanically-driven
stem cell separation in tissues caused by proliferating daughter cells. SciPost
Physics. SciPost Foundation. https://doi.org/10.21468/scipostphys.16.4.097
chicago: Krämer, Johannes C., Edouard B Hannezo, Gerhard Gompper, and Jens Elgeti.
“Mechanically-Driven Stem Cell Separation in Tissues Caused by Proliferating Daughter
Cells.” SciPost Physics. SciPost Foundation, 2024. https://doi.org/10.21468/scipostphys.16.4.097.
ieee: J. C. Krämer, E. B. Hannezo, G. Gompper, and J. Elgeti, “Mechanically-driven
stem cell separation in tissues caused by proliferating daughter cells,” SciPost
Physics, vol. 16, no. 4. SciPost Foundation, 2024.
ista: Krämer JC, Hannezo EB, Gompper G, Elgeti J. 2024. Mechanically-driven stem
cell separation in tissues caused by proliferating daughter cells. SciPost Physics.
16(4), 097.
mla: Krämer, Johannes C., et al. “Mechanically-Driven Stem Cell Separation in Tissues
Caused by Proliferating Daughter Cells.” SciPost Physics, vol. 16, no.
4, 097, SciPost Foundation, 2024, doi:10.21468/scipostphys.16.4.097.
short: J.C. Krämer, E.B. Hannezo, G. Gompper, J. Elgeti, SciPost Physics 16 (2024).
date_created: 2024-06-03T08:58:44Z
date_published: 2024-04-08T00:00:00Z
date_updated: 2025-09-08T07:45:40Z
day: '08'
ddc:
- '530'
department:
- _id: EdHa
doi: 10.21468/scipostphys.16.4.097
external_id:
arxiv:
- '2310.04272'
isi:
- '001202370200001'
file:
- access_level: open_access
checksum: 6fdeecd21c166db8dedb927ecc2e6025
content_type: application/pdf
creator: dernst
date_created: 2024-06-03T11:18:51Z
date_updated: 2024-06-03T11:18:51Z
file_id: '17109'
file_name: 2024_SciPostPhys_Kraemer.pdf
file_size: 4973291
relation: main_file
success: 1
file_date_updated: 2024-06-03T11:18:51Z
has_accepted_license: '1'
intvolume: ' 16'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
publication: SciPost Physics
publication_identifier:
issn:
- 2542-4653
publication_status: published
publisher: SciPost Foundation
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanically-driven stem cell separation in tissues caused by proliferating
daughter cells
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 16
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '18446'
abstract:
- lang: eng
text: How living systems achieve precision in form and function despite their intrinsic
stochasticity is a fundamental yet ongoing question in biology. We generated morphomaps
of preimplantation embryogenesis in mouse, rabbit, and monkey embryos, and these
morphomaps revealed that although blastomere divisions desynchronized passively,
8-cell embryos converged toward robust three-dimensional shapes. Using topological
analysis and genetic perturbations, we found that embryos progressively changed
their cellular connectivity to a preferred topology, which could be predicted
by a physical model in which actomyosin contractility and noise facilitate topological
transitions, lowering surface energy. This mechanism favored regular embryo packing
and promoted a higher number of inner cells in the 16-cell embryo. Synchronized
division reduced embryo packing and generated substantially more misallocated
cells and fewer inner-cell–mass cells. These findings suggest that stochasticity
in division timing contributes to robust patterning.
acknowledgement: "We are grateful to the members of the Hiiragi laboratory for discussions
and comments on the manuscript: R. Bloehs, S. Friese, S. Hozeifi, L. Pérez, and
W. Schwarzer for their technical support; V. Janssen for establishing the PAB protocol;
members of the Tsukiyama group for the animal care with monkeys, in particular H.
Tsuchiya and M. Nakaya; Unité Commune d’Expérimentation Animale (UCEA, Jouy-en-Josas,
France) for the animal care with rabbits; the EMBL electronic and mechanical workshops
and the EMBL animal facility for their support; We thank Luxendo for the close collaboration
in developing the light-sheet microscopy for mammalian embryos.\r\nFunding: This
work was funded by the following: EMBL Interdisciplinary Postdoc Program (EIPOD)
under Marie Sklodowska Curie Actions COFUND III RTD (to D.F.); JSPS Overseas Research
Fellowship (to T.I.); Field of excellence “Complexity of life in basic research
and innovation” of the University of Graz (to B.C.M.); European Research Council,
ERC Advanced Grant “SelforganisingEmbryo”, grant agreement 742732; ERC Advanced
Grant “COORDINATION” grant agreement 101055287 (to T.H.); Stichting LSH-TKI, grant
LSHM21020 (to T.H.) JSPS KAKENHI grants JP21H05038 and JP22H05166 (to T.H.)"
article_number: eadh1145
article_processing_charge: No
article_type: original
author:
- first_name: Dimitri
full_name: Fabrèges, Dimitri
last_name: Fabrèges
- first_name: Bernat
full_name: Corominas-Murtra, Bernat
id: 43BE2298-F248-11E8-B48F-1D18A9856A87
last_name: Corominas-Murtra
orcid: 0000-0001-9806-5643
- first_name: Prachiti
full_name: Moghe, Prachiti
last_name: Moghe
- first_name: Alison
full_name: Kickuth, Alison
last_name: Kickuth
- first_name: Takafumi
full_name: Ichikawa, Takafumi
last_name: Ichikawa
- first_name: Chizuru
full_name: Iwatani, Chizuru
last_name: Iwatani
- first_name: Tomoyuki
full_name: Tsukiyama, Tomoyuki
last_name: Tsukiyama
- first_name: Nathalie
full_name: Daniel, Nathalie
last_name: Daniel
- first_name: Julie
full_name: Gering, Julie
last_name: Gering
- first_name: Anniek
full_name: Stokkermans, Anniek
last_name: Stokkermans
- first_name: Adrian
full_name: Wolny, Adrian
last_name: Wolny
- first_name: Anna
full_name: Kreshuk, Anna
last_name: Kreshuk
- first_name: Véronique
full_name: Duranthon, Véronique
last_name: Duranthon
- first_name: Virginie
full_name: Uhlman, Virginie
last_name: Uhlman
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Takashi
full_name: Hiiragi, Takashi
last_name: Hiiragi
citation:
ama: Fabrèges D, Corominas-Murtra B, Moghe P, et al. Temporal variability and cell
mechanics control robustness in mammalian embryogenesis. Science. 2024;386(6718).
doi:10.1126/science.adh1145
apa: Fabrèges, D., Corominas-Murtra, B., Moghe, P., Kickuth, A., Ichikawa, T., Iwatani,
C., … Hiiragi, T. (2024). Temporal variability and cell mechanics control robustness
in mammalian embryogenesis. Science. AAAS. https://doi.org/10.1126/science.adh1145
chicago: Fabrèges, Dimitri, Bernat Corominas-Murtra, Prachiti Moghe, Alison Kickuth,
Takafumi Ichikawa, Chizuru Iwatani, Tomoyuki Tsukiyama, et al. “Temporal Variability
and Cell Mechanics Control Robustness in Mammalian Embryogenesis.” Science.
AAAS, 2024. https://doi.org/10.1126/science.adh1145.
ieee: D. Fabrèges et al., “Temporal variability and cell mechanics control
robustness in mammalian embryogenesis,” Science, vol. 386, no. 6718. AAAS,
2024.
ista: Fabrèges D, Corominas-Murtra B, Moghe P, Kickuth A, Ichikawa T, Iwatani C,
Tsukiyama T, Daniel N, Gering J, Stokkermans A, Wolny A, Kreshuk A, Duranthon
V, Uhlman V, Hannezo EB, Hiiragi T. 2024. Temporal variability and cell mechanics
control robustness in mammalian embryogenesis. Science. 386(6718), eadh1145.
mla: Fabrèges, Dimitri, et al. “Temporal Variability and Cell Mechanics Control
Robustness in Mammalian Embryogenesis.” Science, vol. 386, no. 6718, eadh1145,
AAAS, 2024, doi:10.1126/science.adh1145.
short: D. Fabrèges, B. Corominas-Murtra, P. Moghe, A. Kickuth, T. Ichikawa, C. Iwatani,
T. Tsukiyama, N. Daniel, J. Gering, A. Stokkermans, A. Wolny, A. Kreshuk, V. Duranthon,
V. Uhlman, E.B. Hannezo, T. Hiiragi, Science 386 (2024).
corr_author: '1'
date_created: 2024-10-20T22:02:06Z
date_published: 2024-10-11T00:00:00Z
date_updated: 2025-09-08T14:22:13Z
day: '11'
department:
- _id: EdHa
doi: 10.1126/science.adh1145
external_id:
isi:
- '001422132300018'
pmid:
- '39388574'
intvolume: ' 386'
isi: 1
issue: '6718'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://hal.inrae.fr/hal-04447081v1/file/2023.01.24.525420.full.pdf
month: '10'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Science
publication_identifier:
eissn:
- 1095-9203
publication_status: published
publisher: AAAS
quality_controlled: '1'
scopus_import: '1'
status: public
title: Temporal variability and cell mechanics control robustness in mammalian embryogenesis
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 386
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '17269'
abstract:
- lang: eng
text: The directed migration of epithelial cell collectives through coordinated
movements plays a crucial role in various physiological processes and is increasingly
understood at the level of large confluent monolayers. However, numerous processes
rely on the migration of small groups of polarized epithelial clusters in complex
environments, and their responses to external geometries remain poorly understood.
To address this, we cultivate primary epithelial keratocyte tissues on adhesive
microstripes to create autonomous epithelial clusters with well-defined geometries.
We show that their migration efficiency is strongly influenced by the contact
geometry and the orientation of cell–cell contacts with respect to the direction
of migration. A combination of velocity and polarity alignment with contact regulation
of locomotion in an active matter model captures quantitatively the experimental
data. Furthermore, we predict that this combination of rules enables efficient
navigation in complex geometries, which we confirm experimentally. Altogether,
our findings provide a conceptual framework for extracting the interaction rules
of active systems from their interaction with physical boundaries, as well as
design principles for collective navigation in complex microenvironments.
acknowledgement: M.L., E.V. and S.G. acknowledge funding from the European Regional
Development Fund (ERDF) Prostem Research Project (No. 1510614, Wallonia DG06), the
Epiforce Project of the National Fund for Scientific Research, Belgium (FRS-FNRS;
Project No. T.0092.21), the Cellsqueezer Project of FRS-FNRS (Project No. J.0061.23),
the Optopattern Project of FRS-FNRS (Project no. U.NO26.22) and the Interreg MAT(T)ISSE
project, which is financially supported by Interreg France-Wallonie-Vlaanderen,
ERDF). A.R. and M.L. are financially supported by FRS-FNRS as a research fellow
(Aspirant FNRS) and Postdoctoral Researcher (Chargée de Recherches FNRS), respectively.
E.V. and Y.K. are financially supported by FRS-FNRS through grants from the Fund
for Research Training in Industry and Agriculture (FRIA). This project was supported
by the European Research Council under the European Union’s Horizon 2020 Research
and Innovation Programme (Grant Agreement No. 851288 to E.H.) and Marie Skłodowska-Curie
Actions (Grant Agreement No. 797621 to M.G.-G.). D.B.B. was supported by the NOMIS
foundation as a NOMIS fellow and by the European Molecular Biology Organization
(Postdoctoral Fellowship ALTF 343-2022) and performed this work in part at the Aspen
Center for Physics, which is supported by the National Science Foundation (Grant
No. PHY-1607611). X.T. and M.G.-G. acknowledge support from the Government of Catalonia
(Grant No. AGAUR SGR-2017-01602 and a CERCA Programme), the Spanish Ministry for
Science and Innovation and ERDF (Grant No. PGC2018-099645-B-I00), the European Research
Council (Grant No. Adv-883739), Fundació la Marató de TV3 (201903-30-31-32), the
European Commission (Grant No. H2020-FETPROACT-01-2016-731957), La Caixa Foundation
and the Biomedical Research Center Consortium in Red (Grant No. CB15/00153) at the
Carlos III Health Institute, Ministry of Science and Innovation. IBEC is recipient
of a Severo Ochoa Award of Excellence from the Spanish Ministry of Economy, Trade
and Business.
article_processing_charge: No
article_type: original
author:
- first_name: Eléonore
full_name: Vercruysse, Eléonore
last_name: Vercruysse
- first_name: David
full_name: Brückner, David
id: e1e86031-6537-11eb-953a-f7ab92be508d
last_name: Brückner
orcid: 0000-0001-7205-2975
- first_name: Manuel
full_name: Gómez-González, Manuel
last_name: Gómez-González
- first_name: Alexandre
full_name: Remson, Alexandre
last_name: Remson
- first_name: Marine
full_name: Luciano, Marine
last_name: Luciano
- first_name: Yohalie
full_name: Kalukula, Yohalie
last_name: Kalukula
- first_name: Leone
full_name: Rossetti, Leone
last_name: Rossetti
- first_name: Xavier
full_name: Trepat, Xavier
last_name: Trepat
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Sylvain
full_name: Gabriele, Sylvain
last_name: Gabriele
citation:
ama: Vercruysse E, Brückner D, Gómez-González M, et al. Geometry-driven migration
efficiency of autonomous epithelial cell clusters. Nature Physics. 2024;20:1492-1500.
doi:10.1038/s41567-024-02532-x
apa: Vercruysse, E., Brückner, D., Gómez-González, M., Remson, A., Luciano, M.,
Kalukula, Y., … Gabriele, S. (2024). Geometry-driven migration efficiency of autonomous
epithelial cell clusters. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-024-02532-x
chicago: Vercruysse, Eléonore, David Brückner, Manuel Gómez-González, Alexandre
Remson, Marine Luciano, Yohalie Kalukula, Leone Rossetti, Xavier Trepat, Edouard
B Hannezo, and Sylvain Gabriele. “Geometry-Driven Migration Efficiency of Autonomous
Epithelial Cell Clusters.” Nature Physics. Springer Nature, 2024. https://doi.org/10.1038/s41567-024-02532-x.
ieee: E. Vercruysse et al., “Geometry-driven migration efficiency of autonomous
epithelial cell clusters,” Nature Physics, vol. 20. Springer Nature, pp.
1492–1500, 2024.
ista: Vercruysse E, Brückner D, Gómez-González M, Remson A, Luciano M, Kalukula
Y, Rossetti L, Trepat X, Hannezo EB, Gabriele S. 2024. Geometry-driven migration
efficiency of autonomous epithelial cell clusters. Nature Physics. 20, 1492–1500.
mla: Vercruysse, Eléonore, et al. “Geometry-Driven Migration Efficiency of Autonomous
Epithelial Cell Clusters.” Nature Physics, vol. 20, Springer Nature, 2024,
pp. 1492–500, doi:10.1038/s41567-024-02532-x.
short: E. Vercruysse, D. Brückner, M. Gómez-González, A. Remson, M. Luciano, Y.
Kalukula, L. Rossetti, X. Trepat, E.B. Hannezo, S. Gabriele, Nature Physics 20
(2024) 1492–1500.
corr_author: '1'
date_created: 2024-07-16T12:32:17Z
date_published: 2024-09-01T00:00:00Z
date_updated: 2025-09-08T08:28:31Z
day: '01'
department:
- _id: EdHa
doi: 10.1038/s41567-024-02532-x
ec_funded: 1
external_id:
isi:
- '001250246200004'
intvolume: ' 20'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2022.07.17.500364
month: '09'
oa: 1
oa_version: Preprint
page: 1492-1500
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
- _id: 34e2a5b5-11ca-11ed-8bc3-b2265616ef0b
grant_number: ALTF 343-2022
name: A mechano-chemical theory for stem cell fate decisions in organoid development
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on ISTA website
relation: press_release
url: https://ista.ac.at/en/news/a-railroad-of-cells/
scopus_import: '1'
status: public
title: Geometry-driven migration efficiency of autonomous epithelial cell clusters
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 20
year: '2024'
...
---
_id: '12162'
abstract:
- lang: eng
text: Homeostatic balance in the intestinal epithelium relies on a fast cellular
turnover, which is coordinated by an intricate interplay between biochemical signalling,
mechanical forces and organ geometry. We review recent modelling approaches that
have been developed to understand different facets of this remarkable homeostatic
equilibrium. Existing models offer different, albeit complementary, perspectives
on the problem. First, biomechanical models aim to explain the local and global
mechanical stresses driving cell renewal as well as tissue shape maintenance.
Second, compartmental models provide insights into the conditions necessary to
keep a constant flow of cells with well-defined ratios of cell types, and how
perturbations can lead to an unbalance of relative compartment sizes. A third
family of models address, at the cellular level, the nature and regulation of
stem fate choices that are necessary to fuel cellular turnover. We also review
how these different approaches are starting to be integrated together across scales,
to provide quantitative predictions and new conceptual frameworks to think about
the dynamics of cell renewal in complex tissues.
acknowledgement: "This work received funding from the ERC under the European Union’s
Horizon 2020 research and innovation programme (grant agreement No. 851288 to E.H.).\r\nB.
C-M wants to acknowledge the support of the field of excellence Complexity of Life,
in Basic Research and Innovation of the University of Graz."
article_processing_charge: Yes (via OA deal)
article_type: review
author:
- first_name: Bernat
full_name: Corominas-Murtra, Bernat
id: 43BE2298-F248-11E8-B48F-1D18A9856A87
last_name: Corominas-Murtra
orcid: 0000-0001-9806-5643
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
citation:
ama: Corominas-Murtra B, Hannezo EB. Modelling the dynamics of mammalian gut homeostasis.
Seminars in Cell & Developmental Biology. 2023;150-151:58-65. doi:10.1016/j.semcdb.2022.11.005
apa: Corominas-Murtra, B., & Hannezo, E. B. (2023). Modelling the dynamics of
mammalian gut homeostasis. Seminars in Cell & Developmental Biology.
Elsevier. https://doi.org/10.1016/j.semcdb.2022.11.005
chicago: Corominas-Murtra, Bernat, and Edouard B Hannezo. “Modelling the Dynamics
of Mammalian Gut Homeostasis.” Seminars in Cell & Developmental Biology.
Elsevier, 2023. https://doi.org/10.1016/j.semcdb.2022.11.005.
ieee: B. Corominas-Murtra and E. B. Hannezo, “Modelling the dynamics of mammalian
gut homeostasis,” Seminars in Cell & Developmental Biology, vol. 150–151.
Elsevier, pp. 58–65, 2023.
ista: Corominas-Murtra B, Hannezo EB. 2023. Modelling the dynamics of mammalian
gut homeostasis. Seminars in Cell & Developmental Biology. 150–151, 58–65.
mla: Corominas-Murtra, Bernat, and Edouard B. Hannezo. “Modelling the Dynamics of
Mammalian Gut Homeostasis.” Seminars in Cell & Developmental Biology,
vol. 150–151, Elsevier, 2023, pp. 58–65, doi:10.1016/j.semcdb.2022.11.005.
short: B. Corominas-Murtra, E.B. Hannezo, Seminars in Cell & Developmental Biology
150–151 (2023) 58–65.
corr_author: '1'
date_created: 2023-01-12T12:09:47Z
date_published: 2023-12-02T00:00:00Z
date_updated: 2025-04-14T07:52:27Z
day: '02'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1016/j.semcdb.2022.11.005
ec_funded: 1
external_id:
isi:
- '001053522200001'
pmid:
- '36470715'
file:
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checksum: c619887cf130f4649bf3035417186004
content_type: application/pdf
creator: dernst
date_created: 2024-01-08T10:16:04Z
date_updated: 2024-01-08T10:16:04Z
file_id: '14741'
file_name: 2023_SeminarsCellDevBiology_CorominasMurtra.pdf
file_size: 1343750
relation: main_file
success: 1
file_date_updated: 2024-01-08T10:16:04Z
has_accepted_license: '1'
isi: 1
keyword:
- Cell Biology
- Developmental Biology
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 58-65
pmid: 1
project:
- _id: 05943252-7A3F-11EA-A408-12923DDC885E
call_identifier: H2020
grant_number: '851288'
name: Design Principles of Branching Morphogenesis
publication: Seminars in Cell & Developmental Biology
publication_identifier:
issn:
- 1084-9521
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Modelling the dynamics of mammalian gut homeostasis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 150-151
year: '2023'
...
---
_id: '12428'
abstract:
- lang: eng
text: The mammary gland consists of a bilayered epithelial structure with an extensively
branched morphology. The majority of this epithelial tree is laid down during
puberty, during which actively proliferating terminal end buds repeatedly elongate
and bifurcate to form the basic structure of the ductal tree. Mammary ducts consist
of a basal and luminal cell layer with a multitude of identified sub-lineages
within both layers. The understanding of how these different cell lineages are
cooperatively driving branching morphogenesis is a problem of crossing multiple
scales, as this requires information on the macroscopic branched structure of
the gland, as well as data on single-cell dynamics driving the morphogenic program.
Here we describe a method to combine genetic lineage tracing with whole-gland
branching analysis. Quantitative data on the global organ structure can be used
to derive a model for mammary gland branching morphogenesis and provide a backbone
on which the dynamics of individual cell lineages can be simulated and compared
to lineage-tracing approaches. Eventually, these quantitative models and experiments
allow to understand the couplings between the macroscopic shape of the mammary
gland and the underlying single-cell dynamics driving branching morphogenesis.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Colinda L.G.J.
full_name: Scheele, Colinda L.G.J.
last_name: Scheele
citation:
ama: 'Hannezo EB, Scheele CLGJ. A Guide Toward Multi-scale and Quantitative Branching
Analysis in the Mammary Gland. In: Margadant C, ed. Cell Migration in Three
Dimensions. Vol 2608. MIMB. Springer Nature; 2023:183-205. doi:10.1007/978-1-0716-2887-4_12'
apa: Hannezo, E. B., & Scheele, C. L. G. J. (2023). A Guide Toward Multi-scale
and Quantitative Branching Analysis in the Mammary Gland. In C. Margadant (Ed.),
Cell Migration in Three Dimensions (Vol. 2608, pp. 183–205). Springer Nature.
https://doi.org/10.1007/978-1-0716-2887-4_12
chicago: Hannezo, Edouard B, and Colinda L.G.J. Scheele. “A Guide Toward Multi-Scale
and Quantitative Branching Analysis in the Mammary Gland.” In Cell Migration
in Three Dimensions, edited by Coert Margadant, 2608:183–205. MIMB. Springer
Nature, 2023. https://doi.org/10.1007/978-1-0716-2887-4_12.
ieee: E. B. Hannezo and C. L. G. J. Scheele, “A Guide Toward Multi-scale and Quantitative
Branching Analysis in the Mammary Gland,” in Cell Migration in Three Dimensions,
vol. 2608, C. Margadant, Ed. Springer Nature, 2023, pp. 183–205.
ista: 'Hannezo EB, Scheele CLGJ. 2023.A Guide Toward Multi-scale and Quantitative
Branching Analysis in the Mammary Gland. In: Cell Migration in Three Dimensions.
Methods in Molecular Biology, vol. 2608, 183–205.'
mla: Hannezo, Edouard B., and Colinda L. G. J. Scheele. “A Guide Toward Multi-Scale
and Quantitative Branching Analysis in the Mammary Gland.” Cell Migration in
Three Dimensions, edited by Coert Margadant, vol. 2608, Springer Nature, 2023,
pp. 183–205, doi:10.1007/978-1-0716-2887-4_12.
short: E.B. Hannezo, C.L.G.J. Scheele, in:, C. Margadant (Ed.), Cell Migration in
Three Dimensions, Springer Nature, 2023, pp. 183–205.
corr_author: '1'
date_created: 2023-01-29T23:00:58Z
date_published: 2023-01-19T00:00:00Z
date_updated: 2024-10-09T21:04:04Z
day: '19'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1007/978-1-0716-2887-4_12
editor:
- first_name: Coert
full_name: Margadant, Coert
last_name: Margadant
external_id:
pmid:
- '36653709'
file:
- access_level: open_access
checksum: aec1b8d3ba938ddf9d8fcb777f3c38ee
content_type: application/pdf
creator: dernst
date_created: 2023-02-03T10:56:39Z
date_updated: 2023-02-03T10:56:39Z
file_id: '12500'
file_name: 2023_MIMB_Hannezo.pdf
file_size: 826598
relation: main_file
success: 1
file_date_updated: 2023-02-03T10:56:39Z
has_accepted_license: '1'
intvolume: ' 2608'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 183-205
pmid: 1
publication: Cell Migration in Three Dimensions
publication_identifier:
eisbn:
- '9781071628874'
eissn:
- 1940-6029
isbn:
- '9781071628867'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary
Gland
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2608
year: '2023'
...
---
_id: '12710'
abstract:
- lang: eng
text: Surface curvature both emerges from, and influences the behavior of, living
objects at length scales ranging from cell membranes to single cells to tissues
and organs. The relevance of surface curvature in biology is supported by numerous
experimental and theoretical investigations in recent years. In this review, first,
a brief introduction to the key ideas of surface curvature in the context of biological
systems is given and the challenges that arise when measuring surface curvature
are discussed. Giving an overview of the emergence of curvature in biological
systems, its significance at different length scales becomes apparent. On the
other hand, summarizing current findings also shows that both single cells and
entire cell sheets, tissues or organisms respond to curvature by modulating their
shape and their migration behavior. Finally, the interplay between the distribution
of morphogens or micro-organisms and the emergence of curvature across length
scales is addressed with examples demonstrating these key mechanistic principles
of morphogenesis. Overall, this review highlights that curved interfaces are not
merely a passive by-product of the chemical, biological, and mechanical processes
but that curvature acts also as a signal that co-determines these processes.
acknowledgement: B.S. and A.R. contributed equally to this work. A.P.G.C. and P.R.F.
acknowledge the funding from Fundação para a Ciência e Tecnologia (Portugal), through
IDMEC, under LAETA project UIDB/50022/2020. T.H.V.P. acknowledges the funding from
Fundação para a Ciência e Tecnologia (Portugal), through Ph.D. Grant 2020.04417.BD.
A.S. acknowledges that this work was partially supported by the ATTRACT Investigator
Grant (no. A17/MS/11572821/MBRACE, to A.S.) from the Luxembourg National Research
Fund. The author thanks Gerardo Ceada for his help in the graphical representations.
N.A.K. acknowledges support from the European Research Council (grant 851960) and
the Gravitation Program “Materials Driven Regeneration,” funded by the Netherlands
Organization for Scientific Research (024.003.013). M.B.A. acknowledges support
from the French National Research Agency (grant ANR-201-8-CE1-3-0008 for the project
“Epimorph”). G.E.S.T. acknowledges funding by the Australian Research Council through
project DP200102593. A.C. acknowledges the funding from the Deutsche Forschungsgemeinschaft
(DFG) Emmy Noether Grant CI 203/-2 1, the Spanish Ministry of Science and Innovation
(PID2021-123013O-BI00) and the IKERBASQUE Basque Foundation for Science.
article_number: '2206110'
article_processing_charge: No
article_type: review
author:
- first_name: Barbara
full_name: Schamberger, Barbara
last_name: Schamberger
- first_name: Ricardo
full_name: Ziege, Ricardo
last_name: Ziege
- first_name: Karine
full_name: Anselme, Karine
last_name: Anselme
- first_name: Martine
full_name: Ben Amar, Martine
last_name: Ben Amar
- first_name: Michał
full_name: Bykowski, Michał
last_name: Bykowski
- first_name: André P.G.
full_name: Castro, André P.G.
last_name: Castro
- first_name: Amaia
full_name: Cipitria, Amaia
last_name: Cipitria
- first_name: Rhoslyn A.
full_name: Coles, Rhoslyn A.
last_name: Coles
- first_name: Rumiana
full_name: Dimova, Rumiana
last_name: Dimova
- first_name: Michaela
full_name: Eder, Michaela
last_name: Eder
- first_name: Sebastian
full_name: Ehrig, Sebastian
last_name: Ehrig
- first_name: Luis M.
full_name: Escudero, Luis M.
last_name: Escudero
- first_name: Myfanwy E.
full_name: Evans, Myfanwy E.
last_name: Evans
- first_name: Paulo R.
full_name: Fernandes, Paulo R.
last_name: Fernandes
- first_name: Peter
full_name: Fratzl, Peter
last_name: Fratzl
- first_name: Liesbet
full_name: Geris, Liesbet
last_name: Geris
- first_name: Notburga
full_name: Gierlinger, Notburga
last_name: Gierlinger
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Aleš
full_name: Iglič, Aleš
last_name: Iglič
- first_name: Jacob J.K.
full_name: Kirkensgaard, Jacob J.K.
last_name: Kirkensgaard
- first_name: Philip
full_name: Kollmannsberger, Philip
last_name: Kollmannsberger
- first_name: Łucja
full_name: Kowalewska, Łucja
last_name: Kowalewska
- first_name: Nicholas A.
full_name: Kurniawan, Nicholas A.
last_name: Kurniawan
- first_name: Ioannis
full_name: Papantoniou, Ioannis
last_name: Papantoniou
- first_name: Laurent
full_name: Pieuchot, Laurent
last_name: Pieuchot
- first_name: Tiago H.V.
full_name: Pires, Tiago H.V.
last_name: Pires
- first_name: Lars D.
full_name: Renner, Lars D.
last_name: Renner
- first_name: Andrew O.
full_name: Sageman-Furnas, Andrew O.
last_name: Sageman-Furnas
- first_name: Gerd E.
full_name: Schröder-Turk, Gerd E.
last_name: Schröder-Turk
- first_name: Anupam
full_name: Sengupta, Anupam
last_name: Sengupta
- first_name: Vikas R.
full_name: Sharma, Vikas R.
last_name: Sharma
- first_name: Antonio
full_name: Tagua, Antonio
last_name: Tagua
- first_name: Caterina
full_name: Tomba, Caterina
last_name: Tomba
- first_name: Xavier
full_name: Trepat, Xavier
last_name: Trepat
- first_name: Sarah L.
full_name: Waters, Sarah L.
last_name: Waters
- first_name: Edwina F.
full_name: Yeo, Edwina F.
last_name: Yeo
- first_name: Andreas
full_name: Roschger, Andreas
last_name: Roschger
- first_name: Cécile M.
full_name: Bidan, Cécile M.
last_name: Bidan
- first_name: John W.C.
full_name: Dunlop, John W.C.
last_name: Dunlop
citation:
ama: 'Schamberger B, Ziege R, Anselme K, et al. Curvature in biological systems:
Its quantification, emergence, and implications across the scales. Advanced
Materials. 2023;35(13). doi:10.1002/adma.202206110'
apa: 'Schamberger, B., Ziege, R., Anselme, K., Ben Amar, M., Bykowski, M., Castro,
A. P. G., … Dunlop, J. W. C. (2023). Curvature in biological systems: Its quantification,
emergence, and implications across the scales. Advanced Materials. Wiley.
https://doi.org/10.1002/adma.202206110'
chicago: 'Schamberger, Barbara, Ricardo Ziege, Karine Anselme, Martine Ben Amar,
Michał Bykowski, André P.G. Castro, Amaia Cipitria, et al. “Curvature in Biological
Systems: Its Quantification, Emergence, and Implications across the Scales.” Advanced
Materials. Wiley, 2023. https://doi.org/10.1002/adma.202206110.'
ieee: 'B. Schamberger et al., “Curvature in biological systems: Its quantification,
emergence, and implications across the scales,” Advanced Materials, vol.
35, no. 13. Wiley, 2023.'
ista: 'Schamberger B, Ziege R, Anselme K, Ben Amar M, Bykowski M, Castro APG, Cipitria
A, Coles RA, Dimova R, Eder M, Ehrig S, Escudero LM, Evans ME, Fernandes PR, Fratzl
P, Geris L, Gierlinger N, Hannezo EB, Iglič A, Kirkensgaard JJK, Kollmannsberger
P, Kowalewska Ł, Kurniawan NA, Papantoniou I, Pieuchot L, Pires THV, Renner LD,
Sageman-Furnas AO, Schröder-Turk GE, Sengupta A, Sharma VR, Tagua A, Tomba C,
Trepat X, Waters SL, Yeo EF, Roschger A, Bidan CM, Dunlop JWC. 2023. Curvature
in biological systems: Its quantification, emergence, and implications across
the scales. Advanced Materials. 35(13), 2206110.'
mla: 'Schamberger, Barbara, et al. “Curvature in Biological Systems: Its Quantification,
Emergence, and Implications across the Scales.” Advanced Materials, vol.
35, no. 13, 2206110, Wiley, 2023, doi:10.1002/adma.202206110.'
short: B. Schamberger, R. Ziege, K. Anselme, M. Ben Amar, M. Bykowski, A.P.G. Castro,
A. Cipitria, R.A. Coles, R. Dimova, M. Eder, S. Ehrig, L.M. Escudero, M.E. Evans,
P.R. Fernandes, P. Fratzl, L. Geris, N. Gierlinger, E.B. Hannezo, A. Iglič, J.J.K.
Kirkensgaard, P. Kollmannsberger, Ł. Kowalewska, N.A. Kurniawan, I. Papantoniou,
L. Pieuchot, T.H.V. Pires, L.D. Renner, A.O. Sageman-Furnas, G.E. Schröder-Turk,
A. Sengupta, V.R. Sharma, A. Tagua, C. Tomba, X. Trepat, S.L. Waters, E.F. Yeo,
A. Roschger, C.M. Bidan, J.W.C. Dunlop, Advanced Materials 35 (2023).
date_created: 2023-03-05T23:01:06Z
date_published: 2023-03-29T00:00:00Z
date_updated: 2023-09-26T10:56:46Z
day: '29'
ddc:
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department:
- _id: EdHa
doi: 10.1002/adma.202206110
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title: 'Curvature in biological systems: Its quantification, emergence, and implications
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