[{"oa":1,"_id":"20470","ddc":["579"],"degree_awarded":"PhD","day":"30","file_date_updated":"2026-04-30T22:30:02Z","supervisor":[{"last_name":"Guet","first_name":"Calin C","full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87"}],"year":"2025","publication_status":"published","author":[{"last_name":"Wu","first_name":"Bryan","full_name":"Wu, Bryan","id":"3C521EBA-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2025-10-15T13:30:21Z","date_updated":"2026-05-06T08:01:28Z","date_published":"2025-10-30T00:00:00Z","status":"public","language":[{"iso":"eng"}],"publication_identifier":{"issn":["2663-337X"]},"file":[{"file_id":"20516","date_updated":"2026-04-30T22:30:02Z","creator":"brwu","date_created":"2025-10-21T17:33:27Z","file_name":"2025_Wu_Bryan_Thesis.docx","checksum":"d32ea83f259f6b0506325cd57b1d44c3","file_size":10603235,"embargo_to":"open_access","access_level":"closed","relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document"},{"content_type":"application/pdf","relation":"main_file","access_level":"open_access","checksum":"53590046fd3244c5550b4022282449d2","file_size":6251936,"embargo":"2026-04-30","file_name":"2025_Wu_Bryan_Thesis.pdf","file_id":"20517","date_updated":"2026-04-30T22:30:02Z","creator":"brwu","date_created":"2025-10-21T17:33:26Z"}],"alternative_title":["ISTA Thesis"],"OA_place":"publisher","corr_author":"1","month":"10","article_processing_charge":"No","publisher":"Institute of Science and Technology Austria","page":"102","oa_version":"Published Version","citation":{"short":"B. Wu, An Examination on Phages as a Naturally Composable System, Institute of Science and Technology Austria, 2025.","chicago":"Wu, Bryan. “An Examination on Phages as a Naturally Composable System.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-20470\">https://doi.org/10.15479/AT-ISTA-20470</a>.","ista":"Wu B. 2025. An examination on phages as a naturally composable system. Institute of Science and Technology Austria.","apa":"Wu, B. (2025). <i>An examination on phages as a naturally composable system</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-20470\">https://doi.org/10.15479/AT-ISTA-20470</a>","mla":"Wu, Bryan. <i>An Examination on Phages as a Naturally Composable System</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20470\">10.15479/AT-ISTA-20470</a>.","ama":"Wu B. An examination on phages as a naturally composable system. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-20470\">10.15479/AT-ISTA-20470</a>","ieee":"B. Wu, “An examination on phages as a naturally composable system,” Institute of Science and Technology Austria, 2025."},"type":"dissertation","abstract":[{"text":"Systems design has classically relied on composable systems, in which individual subsystems\r\nhave defined inputs, outputs, and interactions with each other; however, attempts at\r\ndesigning complex systems in synthetic biology has often run in to issues of crosstalk and\r\ninterference, given that these systems must function within the context of the host. In nature,\r\nmobile genetic elements are systems that have evolved to travel between hosts, and thus\r\nappear to be a good candidate with which to evaluate composability. Selecting temperate\r\nphages as a model system, I used mathematical modelling to identify sources of information\r\nthat temperate phages should respond to. I found that essential proteins of temperate phages\r\ncan interfere with potential hosts, indicating limitations to composability. I also designed a\r\nlysogeny reporter construct and characterize its behavior across various laboratory and\r\nenvironmental strains, finding differences in phage lambda lysogens, and potential\r\ninterference from prophages that already exist within the environmental strains. Although\r\nthe information gathered is not conclusive, it suggests that composability is not a key property\r\nof temperate phages, implying that biological systems may not be composable, and that other\r\nsystem design principles should be considered when designing synthetic systems.","lang":"eng"}],"title":"An examination on phages as a naturally composable system","has_accepted_license":"1","department":[{"_id":"GradSch"},{"_id":"CaGu"}],"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","doi":"10.15479/AT-ISTA-20470"}]